High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by erb
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 97282
Chr: 7
Strand: 1
Status: Ordered
BACs: RP24-276J8 : RP24-328M12 : RP23-390L14 : RP23-245E21 : RP23-368F23 : RP23-238I10 : RP23-98O19 :
Instance: Plate 105: Well H08: BACs RP23-390L14 RP23-245E21 RP24-328M12 RP24-276J8
Oligos on GRCm38:
Type Start End Length Seq
G5 131537987 131538036 50 AAGTAAGAAAAGAACTCTTAGGACAGAGCAACCTGTGCATAACTGTGTGT
U5 131543610 131543659 50 AAATTGTCCTCCAGCAGGAATGGGTGGCTTGGGATGGCCTTGGGGGGTGG
U3 131543660 131543709 50 GGGGGAGGCCTGGAAGGCAGGGTTGAACCTTAGCAGTGGAGAATTTTCCC
D5 131545187 131545236 50 AGTTATCCACCACCCACCTTCCAAAATCTGAGCGGCCTCTGGTAAGATCC
D3 131545237 131545286 50 TCACTTGGTCTGTCTGTGGCAGTCAACTCCATTTATCTCCGTTTGGACTG
G3 131549541 131549590 50 TGGAGCAGATGATGTTGACCTTTTATCATACAAACAAGGAGGGTGAAAGA
Oligos:
Type Start End Length Seq
G5 138681501 138681550 50 AAGTAAGAAAAGAACTCTTAGGACAGAGCAACCTGTGCATAACTGTGTGT
U5 138687124 138687173 50 AAATTGTCCTCCAGCAGGAATGGGTGGCTTGGGATGGCCTTGGGGGGTGG
U3 138687174 138687223 50 GGGGGAGGCCTGGAAGGCAGGGTTGAACCTTAGCAGTGGAGAATTTTCCC
D5 138688701 138688750 50 AGTTATCCACCACCCACCTTCCAAAATCTGAGCGGCCTCTGGTAAGATCC
D3 138688751 138688800 50 TCACTTGGTCTGTCTGTGGCAGTCAACTCCATTTATCTCCGTTTGGACTG
G3 138693055 138693104 50 TGGAGCAGATGATGTTGACCTTTTATCATACAAACAAGGAGGGTGAAAGA
G5_U5 138681501 138687173 5673 AAGTAAGAAAAGAACTCTTAGGACAGAGCAACCTGTGCATAACTGTGTGTGGGAGACTCCTGTTTTAGAAGGAAACATTTGAAGACACCAGAAGTAGGAAGATGGAGAGGATGTAGGCAGCCTACCAGGATTAGCTAACCTAGCTTTGTCAGAGAAACCAACAAGAACAGATTTTTTTGGTCTGTGGGGTCATCTTTAATCTTAGCACGTGGGAGGCAGAGGCAAGTTGGAACTCTGTGAAATCTCATTTCAGGCCAACCAGAATGACCCAGTAAGCCCCTGTCTCGAAATAAACCACCAACAAATCAATCAATCAGTCAGTCAGTCAGTCAACCAATCAGTCAATATTTTCCAAGAGTAGAGTGGATAAGAATTTACCATAAGCATAACCAGCAGTCACTGCATGTGTCCCCAAGTGTCTTCATAGCAATGTTAGGAGGCTGGGTCCTCGGGTAAGATCTACTTCCTTTTGTTGTATAATCTTGTGCCTCAGTTTCTTCACTTTCAAGTACCGCCCACCTCTCCCTGGGATAGTGGCCAGTGTGCAGTGAGGACTCAACCAGTGACTGATGCCTAAAATTAGCTCTGCACAGTGAGTTCCCTTATTCTCAGAGAAGGGTTAGTAATTTGGCCACTCTAAGGAGTTTAGTACATATATCAAGATATTTATGTCATCAAGGACTACGTGGCTGCAGTCACTCCCTAAGAGCTATACATTCCTCAAAGTCACACTCATTAGCCAAAGCAGGGGTCTTTGGTAGTATTCTAAGTAGCCCGAGGAAAGATGAGCAGGAACTTCCTGGGAGACAGGGTCAGAGTTTTCCTGGGAATCGCAGCTCTAGGCTTTTGTCCATTTTACCTACAAATCAAAAACCTGTAACTCAGGAGTGTTGGATGGCAAATCTAAGCACCAGGAACAGGACAAACCCTAGCTATTAGTTGTGAAGGCCTCCAGCATGGATCTTGCAACTTTGGCTAAACGCTTAGCTGGTCCCTACCCTTTCTGTAAGTCTGTACACTACATCTGGGGAGGACTCATCCACTCTGGCTGCAGAAGGAAGCCCCCTGCCTTGGGTAAAGTCCTAGAGAACATGTGTACACTCCCCTTTCTTGGGGAACAATCTGCTTAGCAACTTGAGTGTGTGCAAATGCACATAAGACTTTTGCGCGTGGCAGGTGTAGGACACAATTCTAAGCATGCTTTTGCCGAGGCCCATTCTTTGGGTAGACGTTTGGTGTTGATCACTAGTATTCTCCAGGCCCCCTTTGCCCTCTTGGATACTTGTCCCAAGATACAGCAGTGTGAAGATCTGACTGTGCACAGCCTGCGTGCTGAAAATCTGTGAGGCACTCTTCAGCAGTCGTGTTCCTGGAAAAGCCTTTAGTTTTTTGTAAAAATATGTAATCAAAGACCTCAGATTATCTGAGTGTGGTTGCTCATACCTGTAGTTCCAGCATTGGGGAGGCTACATACTGACTTCTGGGTAAGCCTGGGCTACAGACCAAGATCCTATGCCAAAACCCCAAACAAGCTAGATTTTCATTAAACACTTCAAAAACTGTGTTAGCTGGAGTGTTTGGGAGGAAGTTGCCAGGGTGTCTGAAGTACTCTTGTCAAGCAGACCAATCCTTTGCAGTGTGAATTGTGACTCCACCCACCCACCCACCCATCCACCCACGCACCTATCTGTTCATCCATGTACTCGCCCACCCATCACCTAAGTCAAGAATTGAGGCATTAACACAAACCTTTACTGAAACGCCTACTCACTGGCCAAGTGTGCACTGAGTTCTCAAAAGATAAATAAGGCAACTCCTCGTCTCATACAGCAGCATCCTGAAGCTCCAGCAACTGCGGTCAGGCTCCACATGAAAAGAAAAGGCTGCAAGGAGAAAACTAAAACCTGCCTTAAAGGGAGACAGAAAGCCAGAAGAGTGAAAAGCAGGAGAGAACTCTTCCAAAGGTGGCTAGACCTGCGGGAATCTGCAGGAGACGTCCCTTGGTTTCTCTCATAACTCAGTTTTTTTGTTTTTTGTTTTTTTTAAGTGCTGTTTTCTAAGGGTGGTAGGGAATATTAGGGCCTAGGCTTTTAAATTCTTAGCATAGATTCTGTGTTTTCTTCAGGTACTTCTGTAGGCTCGAGGATGCCCCATAGAGGGCGCTGTTTGCCTGGCTTTTAAGGCAGCACGGTGCTGGCAAGTGGAGGGAACAAGACTTTCTGGAGAACAAGTAAATTGCTATCCAGGCACCCGAGCTTTTTCGAACCAGAAAGGAAAGCTCAAGTCAGTGCTTCCTGATGTTGACGTCCAGCTGGAGATGCTGACACCCAGTAAAGAGACCCTTTCCTCTAGTGAATACACACACACACACACACACACACACACACACACACATGCTCACATTAGGAAAGATACATGCTGGATCGGCCCAAGTAAGCTGCCTCTTAACTCTTCTTGGGTCCACACTTCTGGGTCTAGTCCTAGAACTGAGATCGGCCAGAAGCCGTGGACCGAATGTCTTTCACGCCTGTCTTCCTGGGACAGATATGTCTTTCAGGTCTACGGACTCATAGATGGGTTTGTTGCGGGTTTTGGTTTTGGTGTGTTTGTTTGTTTGTTTTTGTTTTGTTTTGAGACACAGTGATTGAACACACGGCCTCTCTGGGTAGGCAGAGAGTAAGGTAGCACTGACTCACAACGACCACCTTTCCGAGAAGTGGAAAGTGTTTCCTCTTTCCCTAATTTAAAAGAGTCCTTGTACATCAGAACTTTAGCAAGCGTGCACACGTCTGCAGCTTCTCCCTCACCAGACCCGCACATACCCTTGGGAGCCTCCTCAGCCTAGTGGGCCGCCAGGTTTGCTGTGCGCAAGCAGTGAGCTCCGAACCGATGTCGTCTATTCAAGAAGAACGATTTCTGCTTTGGGTGGGTGGGTGGGGGTGGCCGCTGCTTGTCCCACACTGTTTGGGCGTCTTAGGTACAGCCTCCTCTCTGGACACCTTTTCTCTATAAAATCTGAGCGTATTACCAACCCTGCTTGAGCCTCGGAGCTGTGATTGGCTCCAACTCGGATTTTAGATGATGCGCCCCTGTGCATTCCAGTAAAGCCTTATACGAAATCAAAGGAATAATAGTAACCATTTATAGAAGCGCTACTTACAAGGCAATAGTAGGGTTCGTTACCGAGGGTGGATGTCAAAACTTTAAATACGACCTTCAAGACTTGGTAGAGGCGGTTCTGGTGCCTTAGGTCTGAAAAATAAAATAAACCTACTTCTCATTTGGGGACCCAAAACTGTCCAGGCTGCGCTAGGTAAGCAAGAGGAGCTTGAATTAGGCAAGACTCAACCGAAAGATAAAGAACGGGCTGAGGCGGAGGAAGTTCAGGTGTGAAGGGATTTTCATCCAGGCTGAGAACAAATTGTCATCTTTCTCGGGCCAAGGAACTATCACCAGGGGAAAGGACCCCATGGAAATCTAGAATTGAACTGATTGTCTCTCCGGCTTCCTGCTTGACCCAGGGAGCTGTTAAGGTCGAATGAGTCCAGCTATTCCTCAGAGGCCCGCCGAGATTCTCCAGGGACTTGGAGGCCCTTGGGACATGCTGCAGGTGTCGTTGCCAGGGTCACAAACGAGAAAGGTACTCTTCTGCGCCTTCGGTTCTTGGCGCCTATATTAAAGCATTAGGCAGTTCCAAATGCCACCCTTTGGGGGGAAAGTGCTGTGTTCAGATCGCAGGCTTAAAGTTGATCTTTGAGCCGCGGACACAGTCACCAAGAAAAGAGCGGTGTCCTTCGGAGTACAGTTGTCAGTGAGGAAACACCGCTACCCCGACAGTTGCACAGGGGAAGCGCGTGCACAGAGTCGCATACCCAACTCCTACTCCTGAAAGATTCAGAATCACTCATCCATTTTCAGGACCAAAGGCCAAGGCTACGCGGATTCCTAAGTCGGCAGACTCTCGACTAGACGAAGAGCGGGGAAGCTCTTTTTGGCCGCACCCTGATTAGCAGGTTTGGGGAAAGAAAGTACTGAGTTCTTTCGGGGCCGGGGAGGCGCCCTACTTCCTGGACCCGCACGGCCAAGGCTTTCCAGCCGCGTGGGGCAGCTGGCCTCTGCCAGGCCGGGAGGGGTGGGAGAGCGGCCGCTGCTAGCGCGGATAACCCGGAGGTGACCCAGGCCAGCCTGCTCTGCCCCTCCCCCTCAGGCAGACCGGACAGCAGCCAGCCCGCAGCCGAGCTGCAGGGGGTAGAGGGAGGTGCTTGCTCCAGACTGGAGCAGGTCCGTGCGCTCCGGCCCGCCGGGGGCGTCTCCTTCTTGAGGTCCCTCGCGCGCCTCCCGCTTGGTGGGAGCTCTCAGAGTGCCTCTTTAGCGAGAGCCAATATCCTTTTGTGCTAATAGGTTTGTCCTCATTTGCTGCCTAATTGGACTATATAAAGCCAATAACAGGCGGGCTCTCATAGCCCGAAGCCAAAGCGCCAAAATCCGAGGGAAGGCGAGGAGGGGGCGGTAGAGGAGGCGGCTGCGGGGCCTGGGCTCGGCTGCGCCTTGGCCGGCCTAATCCCATTTGCCATTGTACGCGCCCAATCGCCGTATACTCCCCGCATTTAACTTGGATGACATTTTGATTTCATCATTAGCATCCGGCGCCGGATTGACGCATCCCCAAGCCGGGGACCGCTCTCGCAGCCTCCTCCCGGGGAGCCGCAGCTCCTACCGGCCCCCTCGCTCCCTCGGCGCAGAGGCAGGTCCGCTCCCCGCCCCTCTGGACCCCTCCCCCCATGCGAGCCCGCCCCGGCCCGGTCCCCACCCCGCCCTCCCGCGGCTCGATTCGCGCCCGGGGCGGCCCCCGACTTTGAGCCCCGTAGTTGAGTTCCGTTTATGGTCTGATTTCCGGCCCCTAGCCTGCTCGCCCCGCCGCCCGCCGCCCGCCTGTCCCGCTCCCGCCCTCCGTGGGACCCGGAGGACTGGGGACCATGCCGGAGCCCGGGCCGGACGCCTCGGGCACTGCCAGCGCGCCGCCCCCGCAGCCACCGCCCCAACCTCCCGCGCCCAAGGAATCCCCGTTCTCCATCAGGAACCTGCTCAACGGAGACCATCACCGGCCGCCCCCGAAGCCTCAGCCGCCCCCACGAACGCTCTTTGCTCCGGCCTCGGCTGCTGCCGCCGCCGCCGCCGCTGCGGCCGCCGCAGCCAAAGGTGCCCTGGAGGGCGCCGCGGGCTTCGCGCTCTCGCAGGTGGGCGACCTGGCTTTCCCCCGCTTTGAGATCCCAGCGCAGAGGTTTGCCCTGCCCGCGCACTACCTGGAGCGCTCCCCGGCCTGGTGGTACCCCTACACCCTGACCCCCGCCGGCGGTCACCTCCCGCGACCAGAAGGTACCGACCTCTCTTTGAACTTTCGTTCTCCCCTCTTTCCCTGTGGGCCGGTCACATTCCCTCTCAGAGATGGTGTCACTGCAGTTAGGGATGCCCCGACACCTGCCAACCAGAGTTTTGGTTAACTTCCTTCGGCCTCTAGCCAGCATCTTTCCTGCGCCGCTCGGCTACGAGGCCAAGGAAACCAATCCCACTTGCTGAGAAAAGAAGTGCAGTTGGAGGCCCGGGCTCTGCGATTCCCGGAAAAAAATTGTCCTCCAGCAGGAATGGGTGGCTTGGGATGGCCTTGGGGGGTGG
U3_D5 138687174 138688750 1577 GGGGGAGGCCTGGAAGGCAGGGTTGAACCTTAGCAGTGGAGAATTTTCCCGCTGGCCCCTGGCGTGGGAAACGCGAGATAAAAGGAGCGGCCCTGATCCCACCATTGCCTGGCCAGTCCTAGAAAGGCGAAGAGATCTTAGATCTTTCTGCCCCACTGCAGCCCAGCGGGGACACGGGAGAACCGAGGCCCAGGGCCAGTAGCCCGAGGCCAACGTGTGGGTTGCGCTTGGTGCTCCCAGATCCCTGCCTCGCTCAAGGCTGTGTCGCTGTGCTTGTGTGTGCGTCCGTTTGTCTGTCTCCCCAGCCTCGGAAAAGGCCCTCCTGCGAGACTCCTCCCCTGCGTCCGGCACCGATCGAGACTCCCCGGAGCCTCTGCTCAAGGCTGACCCCGACCACAAGGAGCTGGACTCCAAGAGCCCGGACGAGATCATTCTGGAAGAGAGCGACTCGGAGGAAGGCAAAAAAGAAGGCGAGGCGGTGCCTGGCGCGGCCGGGACGACCGTAGGGGCGACTACGGCGACACCGGGCTCAGAGGACTGGAAGGCGGGCGCCGAGAGTCCCGAGAAGAAGCCGGCGTGCCGCAAAAAGAAGACGCGCACCGTCTTCTCGCGCAGCCAGGTCTTCCAGCTCGAGTCCACATTCGACATGAAACGCTACCTGAGCAGCTCGGAGCGCGCTGGCCTGGCCGCGTCGCTTCACCTCACCGAGACGCAGGTCAAGATCTGGTTCCAGAATCGCCGCAACAAGTGGAAGCGACAGCTGGCGGCCGAGCTGGAGGCGGCCAACCTGAGCCACGCCGCTGCGCAGCGCATCGTACGCGTGCCCATCCTCTACCACGAGAACTCTGCGGCCGAGGGGGCGGCAGCGGCTGCGGGGGCACCGGTGCCAGTCAGCCAGCCGCTGCTCACCTTCCCGCACCCAGTCTACTACTCGCACCCGGTGGTCTCGTCCGTGCCCCTACTAAGGCCGGTGTGAGATGGGACAGGGGACAGGGAGTGAGCACCCGGCCACCTTTTGGGGACCCCGGAGGAGACTGGGCCGGGCCGAGGGTGCCGGGCTGTGGCCAGCGACCTTGGGCTCACTGCCTTGCCTCCCACCCCCCAATAGCATTTTGTAAGTATTTGCAATGCATTTTCGTGCAATTAATTCCTATGAATTTGAGGCGCTTCTCCTCTTATTTTTGGTTTTGCTTACCATTGAGAGAAAACGGGGTGGGGCAGGGGGGAACGACAAAATTACCAGGTCAAAACTTCCAATTGAAAGTTCTTTAAAAAAAAAAAAAGTTCAGATCTCCCCTTAAATTACCATTGCTCTGTGGCTCTGAGGGTGTATTTTTACAGAAGGAAAATAAGCGAAAAATTCAACTTTTTTTTTTAGTTTATTCATTTTTCTGAGGAACTATTTTCAAAATCCCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGAGAATTCATATCGAATTCATATTAATTCTGGATTTCTGGGTGTGGTTCTTTCCCCACACCCAATCAGTTATCCACCACCCACCTTCCAAAATCTGAGCGGCCTCTGGTAAGATCC
D3_G3 138688751 138693104 4354 TCACTTGGTCTGTCTGTGGCAGTCAACTCCATTTATCTCCGTTTGGACTGAAGAATTCACTAAACAAAATGTTTAAAGGGAAAACACAAAAGCCTCAAATCCAGACCTGTAACAGCTTCATATCTGGCTGAGTGGCAGTGGTCTTACTTATTCCTCGAATTTATTTAGTGGTAATATCACTATTACTACTTTGCCTGCTTCAGAAGTTGGTTTCCTCTCTCACGCTCTCTCTCTCTCTCTCTCTCTCTCTCACTTTCTTTTTTGTAGAAAAAAATACATTTTATCCTCTCCCGGTTTTTTGTTTTTTTGTTTTTACTTTACAACAACCGTTGTGCTTTGGCCTGTGCAATATTGTACAAAATTTCCCTCAAGCCCCGCCCCTTCCTCTGCTGGGATAAAGACCACCACACGGCAGCATAATCATTAATGTAGAGGGAAGATTAAGCAGGAGAGGGGGAAGGAAGAAAGCAAGGTAGGTAGCGAGGAGTGAGCTAGGCAGAGTGGAGCGCTTTAAGTGAAGGACAATCAACGTAGGAGAATCTTAACTTATTTGATAACTGTACAGTTTTCTTGTAAAACCTCCCAACTCAACCGGGCACCTTGCTCTGGTTCCCTGTTGGCTACACCTGTTGTTCTGTTGTTTTCATTCGTCTGTTTCCCCCCTCCCTCCCTCCCTGTCCTCTGCCGGAGTTCAGTCCCTTTATAAGAAAACAAAACAAAACTCCACAAAATATTGTTACACTTAATGTTATTGTGGAATCGAATTAATTAAAAAGAAAAAAATAAGCCTTTCATGTTGCCAGAGTGTGTAACTTTGGATGCTTTCCACGCCCCTGGGAGCAGGTGAGCAGGGAAACTCTCCTTGCCTAGCTCTCTGACTACCTCTTGTGCTCTCTGAGTCTGACCTGCACTCACGCCCCTACAATCCAGCCCGCAGAGGAGACAGGGGCGGCTTTGTTCAGGACCAAGGGCGTTCTTTCCTCTGTATCTTCTGCTGCTTGAAAGGCCCTCGTCAGAGCAAGGAAGAATGAGGCATGGGAAAGGTATAAAGATCCAGGTTTATGTCCAGCTTGCAAATTTGAGCAAAATAACCCTGAGCTTAAAGGCAGGTGGTATTTCCCAAGAAAAAGCTAAAGCTTGCATTCTTTCTTCAAGCCAGGGTTCCTTCAAGAGCTGGACAGGACATCCCTAAGATGTCTGTCTAAGATTGTGGTGCCTGTGATTGTCCGAAGGGGTGTTGGGGAAAAGAAGGGAGCACAAAGCTTGGAATTCTGCTGAATCTTGAGGCAGGTCATTAAACCTAGCAGTCCTTGAGATAGATGGACAAGGAGTTACCACTTCTAGGACAGGGAGACAGCTGAGCTGGGAGAAATTCAAGCATCCAGTGTAGACCTTGTAGAGGATGTGCCACTGGCTGAGAAAGGACTGGAGTTGACTGGTTCCCCCAACCAGGCTTTCTATGCCCATCGGTTCTCAAAGCTGCCCCAACAGATAGCTAGCCTGTCTCCACCCACCTGTTTTAGCCAGGGCTTAGAAGCCTTTTTTCCTTAGGCTACTTCACCCTTGGTTCTCAGCATCAGAGGCAAGGCATTCCAAGACAGCTTAGAACTGGTCTTCTAAGGACCGAGGGAAGTAGGTGTTCAGATGCCCCTTGCCAATTCAACATGCATGCCTGCCTTCAAGCTCTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTCTGCTCATACTCCAGGCCCTGTTCTTTTGGAGCTGAAGAACTAAGCAAGCAATGGCCGAGATTTCAGAGAGCATTGCCATACGCAGGACACAACCACTGGGGAGCGCCTGTACATGCGCTCTTTTCTCCTACTGTGCTGCCAGGCTCCTCCAACTCAATCGCCACAATTGATTCTACTGTGGCTGAAACGCAACTATCGTTTTTTTTTTTTTTTTCTGTTGATGGAAAACTGGCCCCAAATAAACCAGTGTGGGTATTTGTGGCAAAATTTTAGCCTGGACCTCAGTGAAGATAGATCTGCCTTTATTTTCCTCTGGTTTGGAATAAAAACGAGGGCATGTGTCGTAAATGAAAAATAAACAGAATATCAGGGTCAAAATATGTTGTTCCCTGTGTTGCCTGAAAACAGAGTGATGCCTTTTACTGTTGGCAATACTAACTGCGGGACAGGCCAGTAACAGTTCATTAAAAATAATGTTGCAATAGCAAACATGTCCTCAGATTTTACAGCCAGAGCATGATGTTATCAAATGCCAAGAAAATACTTCTTTATTTGTTTGGATTCCAGAAAACGGTTCTTGACTGACCCAGTCTTTTGAAAGTCAAGATGTAACTGGTTCTCTACAAGCGTAGTTTATCCTTTGTGGGAGGCCCCTTGGCTTCACTTTTCTTCCTGCGTGGGAGCTTTGCTGCTCTGGGCTGGTACCTCTCACCCCAGGGCCACAGTGCAGCTCTGGACGATGATGGCCGGTGTTCATACCATCTTCCAGCCCAGTGGGGTCCTGATGGTTTTCTCCCATGTTCAGTGTTTGAGGAGGAAGCCTGTGACATTATGGAATACTGGTGCTGCTCTGAGTGGGGCACAGTGGACCTTGCTGTCAGAAGAAACCCAGTACAGGACCAGAGAGCTGGTTGTCTTCTCCTGTGTAGTCTTTGAAAAGATTCCCAGGAGTTTCCAGAGCCAATTACCAAGAAGACTTGTAGGGAGAGTGACAAGTCCTCCTGAAATCCCTGTGGACGTTCCTTAGGAGAGTTCAGGGCAGCCAGGCTTTCTGTTTCAGGATGCTAAGTGGGCACCTCCTATTTTATCCTTGTACCACCTTCAGGTTGTGGAACCTGGAAACTTTCTCTCTGTCCTACCTACAGTCCTGCACCCAACCTCAACACCAGTCAGTGCTCTCTGGTCAATGAAACCTTCTACAGTGGCCACCAAATAGATGGATGTTCGCAAATAACACAGAATACACTCACTTGACACAGCACACATGGCGATCCAAAGTAGCTCAACACACCCTCTCACAGTTTGTGGTTTTTAGCATCATTCTGATCGCTTAGAGCATACACAGAGTCACGTGCTTTGCTCAGAGCGACTCCAGGTGGCTGGTTCTCTCCAATGGTCCCTGGTTAAACTGAATTGAGAATGGATAGCACAGATGGGCAGGAATAGGGCATCTACCCACCCATCTCAGCTGCATAACCTGAAAATTCCCCATGTGGCAGGTTGTTCCTGGAGTTTCATCACAAGCCACGTAGTCAAACTAGCCTAACCTCACCACCCCTCTACCAGGGACCCTCACAGTCCTGTGTGCCAAGACTATAAGGAGTCCCCACAGAGTTCCAAGCACCTCTGAGTGCCTTCAGGCCCTGGGAGAGCTCAGAGCCAGCCTGTGAACTGTTTCTGTTGGCAACACAGATGTATTTCTTTTATTTGGTAGTAAATAGAGTAGATGTGTGTACATCAAACAGCAGCAAGATATATAAATGGCAACCCGGCCACTGGGGCTCATTCCTAAGGGGGTATGTCCCCTCCGTAGGTCAAAGTCAGAGACCACAAGGAGGGAACCCCAGAGCACCCACCTCTCCTTGCCTGGGAAACTAGAAGTGGAGCTGGGGAGAAGGAGAAGGAGGTGGAAAGGTGGGTGAGATTTATGTCTTTTCTGAAGCCATCCTGTACTCGGCAACTGGGGAGCGGGAGAGAAGGGAGGTTCCAGGAGTCTCTAGGGCTGCTCAATCTGGAAGCTTCTGGGGCTGTAGCACTAGGGATGGAGCATCCTTTTGATTACAGTGTCAGTATCCCCGCTTCCGATCTCTCTCAGCCCCTGGGAGAGCTGAACTGCCTTCCAGAGTCCTGGGGTGGGGTGGGGTGGGGTGGGGTGGGGGGGTGGGGTGGGGTGGGGGGTGGGGTGGGGCCCTAGCCTTTACTGGATGGGCCCTTGACCACTTCAAAGACCTCATTTCATTTCACTTTAGCTTGCACTGTGACTGTCTCCTTTCCGTAGCCCAAACTCTAACTCTGCAAAGTGCCTAGGAAACTGAGCCAAGGCGTCAAGGGTGGTCAGATCCACAGAAATCCTGGAGGCCGCCAGGAAGACCCAGGGGACTCTGCTCACACAAGCATTTGCTTGACCTTAAAGGAGCCATCTGCCTAGCACTTCCTGTTTTTCTCCTGCTCACTCTCTACCAGCTACCGGGCAATGTTTACAGGCCCTCGCTGACTTTAGGGGACTCCACCAGCATGCACACATCTGAAAAGTATACCCGGTTGAGTACACACCGGGTCTTTCCTTGCTGGAGCAGATGATGTTGACCTTTTATCATACAAACAAGGAGGGTGAAAGA
U5_15 138672125 138687173 15049 CGTGACGGGCAGCTCCAGCAATAACAGGAATGTTAAATGTGTGGGGCCTCGGCTGCCTCCTGGGGCCCAGTAGAGGCTTTTTTATTTTATTTTACTTTTTTTTTCTTTTTTCTTTTTAGGAAGAACCGTCACAACTGGCCTTGGGTGTCTGGTGGGGTCTGTGTGTGAAATGGGCTGAGCTTGAAGAGTCATTTAAGGGAATGTTTAACTTCCACTCCCTGTTTCTGAAGAGTAATTTTCTGTAGAGACAGGGAGAATAATAGGAGGAACTTGGCTCATTTTGGCAAATTGCTCCAATTTTCCCACAATCTCAGCCCCCTGTATCACCTGCCCCCCTGTCTATTTGGGCCACTTTGTGCCCTGGATTCAGACCTACTAGAGGCTGTTTAGACTTGTTTTCAGGACAGAAGCTGGAGGAAAGGCCTATCTGGACATCTAAATCACAGCCAGAACTCATTACATAAATGATATATTTACACAATTGATCCCTTTTCTACTGGGTAATTAATCCGGCATCGGAACCCTCAACGATTTAATTGTCATATGCTTAAATGGAAACAGTTGCCACAAATTTCCAAAGACAAGGAGCAGACCGAGGAGGGGGGTGCCTTTTGTTCTAAGATGAGAGAGCTCTCCCTTGTTTCAATGTTGCCACCAAAAGGGAAAGTCACTTCTGTTGGCCCCACAGGAGCAGATGTACAGAGACTGTGCCAGTTTGAGCCTGCCAGTCTCACCTGATCAGGCCATTTAAGGGATGGAGGTGCTGGATGTGGGGGGCACATTTAACCCAGACGTGGTAGAATTGGTCCTGTTCCCACATCGGTAGGACCTTGTTAGCATCCCCACACAATCCTTGGCATGCTCCATACCATCCCCGCGCTGGGTCACATGTGTTCTGCTTCCTGTGGTGACTGCTGGACTCTTCTCAAGGGAATTCATGGAGCCGTTCCATCCAAATAAGCAAAGACCTGGAAAGTGCCTCGAAGTTCACACTGACTAACTACTAGCCTAGAGGAGCAGAACTTCAGAAGACCAAACACTTTTGCTTCAGATGGGAACAACTCTAGAAAGACCATAGCAACTGCCCTGTGGTCAGAATGCATCTGCCTTCTGCAGAACTGTTGGCCGGGAGTACCATGTTTGCCTTCCTCTCTCTCCCCATACCTGTCAGTCTATCTTGGTGTACACCTTAAAAAACAAGTCCTTATCCTAGCATTTGCTATGAGAATCTGACTCAGGACAGTTACTACCTATCTTAGTGCTGTTGGCGCTAGTCTTCCCTCCTCTGTGCTTCAGAGCCATCCTTCATGGAGTAGAAGAAACAGTAACACTAATCCCACCTGGCAAGATGGTTGGTGCTTAAGAAGAATGCACCAGGAACTTCTGTATTAACATCCTTCACGTATCATCTTCTGACCTGCTCAGCACCACGAGAAAGCTGACACTAACATGGATTCTAGAGACAAGGGTATCAAGTAGCAAAGGAACTAAGTACTGTGTCTGTGGTCACACAACTGGCACGATGGTCCAGATGTTTGTGTCCATGATCCCAAATTTATGTATGGAACTTTTTTTAAAGGTATTTTTCTGTGTGATGGATGTTTTGCCTGCACATATATCTGTGTACCACCTATCTGTGTGCAGTGCCTTTGGGGGCCAGAAAAGGCATTGGGTCTATTAGGGCTAGTGTTACAAACACTTGTGAGCTGATGTATGGATGTTGGTGACTGAACCGAGGTCCTCTAAGGTCCTCTGAGAATAGCCAATGCTCTTAACCACTAAGCCCTTGCTCCAGCCCCTGTGTATGGCAATTCTAGTACTCCTGTCCTGGAGTGACTATGAGGTGAGCCCTCTGGCAGGTAATTAGGTCACAAAGATGGCACCTTTATGAATGAGACTAGTGACCTTCTTCTCTAATGCAGTGTAAGAAGTTGGTCACTGTAAACCAGGCAATGACCCCTCATCAGACACAAATGATGCTGCCACCTAACCTCAGACTTCCAACCTCTGGGACTATGGCAAGTGAATGTCACTTTTCAAGCAGCTCAGTCTATAGTATTTTTGTTACAGCATCCAAAAGTGACAAGGACACCCTCAGCTTTTGATCAGCTACCAACTGAGCAACTAGACCCAGATGTCATATGGGCTTTTCTCACACACAGACTCATGTCAACCTCAGACTATGCTGAGACCTATGCTGTCACCCAGATGAAGGATTTGGGACTCATGGAAAGTGGGAAACATGCCCAGATCTACAAGGCCTCTGAGTGATAGTGTTGGGCTCGAAAGCAAAGCATGTCTGAGTCTGATGGACTTGTTCTACCTTGCTTCCCACTCTCCAGCTAAATTCCTGGTGCCCACCAAGGCAAGTAGAACCTACAATGACAGCCTCAGGTGCTAAAGAGATGGCTCAGAGGTTAAGAGTACTTGTTGCTCTTGCAGAAGATCTGGGTTTGATTCCTAGCACCCGTATGACAGTTCACAATTGACTGTAATACCAGTTCCAGGGAATCCAGTGCCTTCTTCTGGTCTTCTTGGGCATCAGACACACATGTAGTGCACAGACATCCATTCAGATATGATACCCATACACATATACAAATTAAAATAATAACATCGTAATAAATAATAATAAAAAGACCAACTTTTCCTTCTTGTGATCTCAGAGGAACTGAGTCTACTGTGAAGACCTCAGCCCGGTGTGGCTGCCACAGCTAGCAAAGTTTACAGAATCCTGACTCCATGTTTCTACCACCACTGGGTTTGGCAGAGGTCTCTTCCTTTTCATCTGTAGGAAGTCAAGGGAAGAAGTCTTTTTGGCCAGATCTGTATTTCATCTCCTGTCCTTTGTCTACACTCCCCGCCACAACCTGCATGGGCACATGCTGCCTGTTTCCTCTACTCTGTCCTTCTTCCTTCCTACTAAATAGCACACCCATCAGTCCCCTGTCCCGATGACGACTTCCTCCAGGAAGCCTCTTCTGCCCACATTCCACCTCTAGAGTAAATCAGAGTAAATCCTCCTTCTATGACTGTGATCACTTGGTGCCTACATATGCTAGACACACTGCCACTGTTAGCCCCTTGTTTTACCTCCCTAGGCTATGAACATCTCAAAGATAGGAGCTGTGACATAGTCAGTTCCTTGTCCTTAGAGCCATAGATGCTATCATTGGCACATAACCTAGAGTGGATGAATGAAGGACTGTCCAGGCTGCTCAACCTTTGCATGTAATCTGGAGGAGGCTGCACCTGATCCTTGACGGTCAATCCTCAGTAGCTGTCCTGAGTTATCCCATGTGACCGTCAATTGTTCCGATGGTACCATGTCCACGGGGCAGGAGAGAATGATGCTCAGGGAGAATGGATTAATCCTCAGCTGCTGAAGCAAGCACCACAGAATCAAAGAACACTGCAGCTAGAGCGGAGACATCGAGCATGACAGTTCTTCATGAACTATGTGGGCACTGGAGTGGCAGAGGTCGTGCTCTGGTGTGACTGGCATGATCCCATTGAGGTGAAGAGGCAAGACTTTCCTTTATTATGTAAGGAGACTGAGCTGACTGTCCCTGCATCCCTTCTTCATGTCTAGCCACCAGCAGAAAGAATCATGTAGTAGCTAGGCAGTGGTGGAAAGTGCCTTTAATCCCAGCACTTGGGAGACAGAGGCTAGCATATCTCTGAGTTCAAGGCAGAGAGGATAGCCAGAGCTGCAAAGAGAAACCTTGTCTTCCAAAGACCACAAAAAAGAAGAAGGAGGAGGAGGAGGAAGAAGAGGAGGAACAGGAGCAGGAAGAGGAGGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAAGAAGAGGGGACAGAGTTTGGGAAAGGTGAAAGAGCCCTAGCTAGGTCATTGAACTAGTTCCATGGGACTCTTAAGACAAGAAACACTGGCTAGGCTACCCTTACACATGAGTAAGAGGCGGGTATCTTTAAGCATCAGCTTCACTGAGATAGCATTTGGGAGTTAGATGATCCTTGTATTTAAAAGAAACAATTTAGTGCCTTTTAACAGTCAAAGCTTCAGACCACCATTACCACAACTGACTTTATTATTATTATCTTAAATTACATTTCAATTGTTGTGTGTATGTGCATGAATGTGGGGGCATGCCACAGCACACCCATGCAGGTCAGACAACTTCTGAGAGTTTTCTCCGTTCACCAGGTGGGTGGGGCCCTGGGGATGCAACTCTTTTGGGTTCTCAAGCGTGTCTGTCATTGCCTTTACACATCGAACCATCTTGCCAGCCTGAGAATGACTTTAGAGCATCACTAAAACCTCCTAAGTGAACCACCAAACCCATTCTCCCTCCCACTGCAGGACAAGCACTTGTCTCCTTGGGGGCAGTCTGAGCTTGGTAGTCACAGAGCTGGAATCACACCCTGTGTGACAGTAATTCTCAACCTATGGATAGCAACTCCTTTGGGGGTCGAACAACCCTTTCACAGGGGTCTCCTAAGACCATTGGAAAACACAGGTATTTACATTATGATTCATAACAGTAGCACAACAACAGTTATGAAGGAGCAGTGAAAATGATCTTATTGTTGGGGGGTCACCACAACATGAGGAACTGTGTTAAAGAGGACCACTGCTCTATGACCACTCCTCCACTTCATGTTTTAAAGAGTCATTTGTGCGGTGGTGTTAAAGGAACTTTACTATTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGTGTGTGTGTGTGTGTGTGTGTTTGTCTGTTTATGTGTCTCTCTCTGTGTATGTGTATGTGTAAAGGGTCACATGTGTGTATTCACATGTGGAGGGGATCAATCATAGGTGTTTTTCTTTGGTCAGTCTTGGTTTGTTGAGACAGTGTCTCACTGGTGTTGATGTATCTCACTGGCCTTTTACTATGTATCGCAATGCTAAATACTGGTCCTCAAGACTTGCTTGCCTCCAGGAATGAGAGAATCTGCAATAGACTCTTGTGTGACACTATGTAACCTTGCCTCCCAAGTTATCCCTGATTGGTGAATGAATAAAGATGCCTACAGCTTATAGCTAGGCAGAAGAGAGATGGGCAGGGTTTCATTTCCTGGGCTTGGGATATAAGGCAGAGACCACGAGGAAGGGAGAGGGAGAAGGTGGAGAGAGAAGACTCCATGGGATAGGTGAGTCACGAAAACTTGGCCATGAGGGCTGGTCAATTGGAGTTAAGAGTTGCCCAGATGGAACATGGCAGATTCTAACTCAAGGTTATTGATGGGTAAGTAGACATACTAGCATAGAGGGTAGATATCTGCCCAGATCTAGTGCTGATTATAAAAATAAAGGTTGTATGTCTTTTATCTGGGAACTAAATGATCAAAGGTGGGGTAGAAACCCTGACTTGAGATTAAATATTAACTGTAACACACTGGGAAGTGGGACTAACCAATATGACTACACGGGTTGGGTGGGGAGCCCCAAAGAAACTTCTATCTCCACTTCCCTGATGTGCCACCATGCCTGGTTTTTTACATGTGTTCTGGAGGTTGAACACAGGTTTTCATGCTTGCACAGCAAACACTCTACAGACTGTGCTGTGCCCTCAGCCCAGTACTTCACTCTCTTTATTGCTGAGTGATATGCAATTAACTAGATATGCCATGTCTTCTTTCCTTTGGGGGAGGGGTGTTGAGTAAAATGGCCCCCATAGGCACAGAGGGAATGGCAGAATTGGGAGGTGTGGCCTTGTTTGGGTGGGCTTTGAGGTCTCAGATGCTCAAGCCAGGCCTAGCCTCTCAGTCTTCCTGCTACCCGCAGATCCAGATGGAGAACTCTCAGCTACTGCTTTAGAACCATGTCTGCCTGGCCTGCATGCTTCCTGCCATAACGATAATGGACTAAACCTCTGAACTGTAAGCCAGTCCAATTAAATTTTTTCCTTCTAAGAGTTATTGTGGTCAGGAGGTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGTTCTTCCAGATGTCCTGAGTTCAATTCCCAGCAGCCACATGGTGGCTCACAACCATCTGTAATGGGATCTGATGCCCTCTTCTGGTTGGGTCTGAAGACAGTTACTGTAGCTCTAACTGACCTGGAAATTGCTGTGTAAACCAGGCTGGCCTCAAACCCAACCTCACAGAGACCTACATGCCTCTGCCTGAGTTCAAAGATGAAAGGCATGTGCCTCCAAGCCTGGTATACCACATACCTTTATCCATTCATAACATTTGCTGTTTTTTTTTTTTTTTAAGAAATCCGAATCCCATCAAAACTTAACAAATCCACAATGGATGTTTATAAGTCATGTGTGACTTTTTACAAGAAACTCTGTCATGAGAATGCTACCCAGAGTGGCAGGGATAAACTATCCACACATTTTGTGTTCAAGAAGACAGACCCAGAAGAGAGAGTGGACATGTCGCTTCTGTGAAATTCTAAAGCTAGAACATTTAACCTTTAACAGCAGTATCAACCCAGTGGCTTCTTGGGTCAGCACTTGCAGGAATTGTCTGCAATGGACAGGGGTGATTTTGGAGGTGTATGCTCAGGGGTGTGCATCTGCCGAAGTCTTATTGAACTGTGTCTTTCAAGGAATGAGATTTCCAACGCACGGTAAATTATACTAAAACGTTGATTATATAGAAGTTACTGGTGCCCATGTGGATCCATGTGTGCTGAATCAAAACAAAAGAAACAAGCCAGGTAGTCATGGAGAAGGAGCTACTATGCATGTAGATTTCAGGTCTTGCCACAAGAGTTCCTGCACATATGCCTTGTGGTGGATACTATTCAAGGAAGAGGGCTAGCAAGCTCATCAGCTCAAGAGTACTTAATCCTGGATGACATCCTGGCACCCATGTCTGACAGCTCACAACTAACAGTTCCAGGGGATCTGACACACTCTTTTGGACTCTGCAGGCACCTGTACACATGATATGCAAACACACACACACACACACACATTAAAAGAACAACAAAAATAAACATAAAATAAACGTAAACAAAGTTGTTTATAAACAAAATAGAAACAAAAACCCCAGTCTACTTTGGGCGACAGATTAAGATCCTGCTTCAGACAGACAAGGAACAAAACATTTGGGTTCCAACTCCACTGCATAAATGGGATACACTACAGATGGTAACTTGAGACTATTCCTGAACAAACTGTCTCTGGAGAGCTATTCTCCATGTCATTATTTCATTATTCATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTATGTGTGTGTGCACGCGCACGTGTGTGTTCCTGTGTTATGTGAGTGTTGGCGTGCATGTATGCCACTATGCACATGTGAAGGTGTCTGCAAACATCCTTGGGGAATCTGTTCTCTTCCACCGAGAGTTCCAAGGATTAAGCTCAAGGTGTCAGAGTTGCCTGGCAAGCTCTTTTACCAGCTGAACCATCTTGCTGCCTGACTCTTCTATTTCGAACTAGTGCCAGGATTATAGGCAGGCACCATCACACCTGTTTTATGCGAGTATGAAACCCAGGGCTTCATGAATGCTAGATAAGCGCTCTACCAATTGAGTTACACCCCCAACTTGTGTCGCTGTTCTAGGTAACAAAATAAGACCTCCAGCCAAGCCTTCTACCAAGGCCTCCAGAGTCACATACGGGTGAGTCCCTTTTTCCTCTCTTGCTAGTCTCCTGTTGGCAACACCACATCTTTGCAGTCTCAGTCTTAGGCACTTCCTTCTGCTTGCGACACTCGTCCTGGCCCCCAACCCCACCACCAAGCATACCTCAAAATATCTGCTTAACCTGCACTTTGGGTATCATGCATGCACATACTCATGCCTGTGCATATCGATGCAGAAGCCAGAGGAGAATAGCAGATATCCTATTGTATCGCCTCTTGCCTTAGTCCCCTTGAGACAGGGTCTCTCACGGAAGCTGAAGCTTGCCAGTTTTCAGCTAGACCGGTTGACCAACAAGCCCCACTGCTTAGATTATAAGTGAGCATTACTGCATCTGGCTTTTTAAAGCATAGATGCTGTGGATTCAAACTCCAGTCATCATGCTTGCCCAGCAAGTACTGAGCAATGAGGCATCACTCAAGTTCAGTGGTTCTCAACCTGTGGGTCACAGCCACAGGGGCAGAATATCAGGTATCCTGCACATCAGATATTTTCATTAGGATTCATAACAGTAGCAAAATTACAGTTATGAAATAGCAATGAAAGTAGTTGTATGGCTAGGGGTCACCACCACAACATGAGGAAATGTATTAAAGGGTCACAGCATTAGTTAGGTTGAGAACCCCTGTTCCAGTTTAACCTCAGTTTCCTAGGGCAGCTTCTCCTGCCTACCTATTTAATACACCGCCATCCTCCCCACCCCCACCGTTTTCCCATTTCCTTTAGCATTTCCCATGACCCTTATCACCTACTTACCCATGCCCAGCTCTAGTGCACTATCCCTAAGGGTAAGACTGCAAGCTCGATGCAGCATCCCAAGTGCCCGGAGTAGTGCTGAGCACACATCTGCTGCTCAACAAGCATCCCAGGCATTGTGATGAAGTTCTTGGCACTTGGTAGGATTTGTTTGGAAAACCCAGCCTGTCTCCACAGTATAAAAGAAATCGAAGAGCAATGTCCACAATGGGAGCCTGTGTGGCTTCAGAGTGAGTACATCACTCCACGAAGGGTGAGCCTTGGGAAGAGATGAGTGTCAAGGTTCACTGGGGACCCCTTTCTGAGAAGCCTCTCCTAATGGTGTCCTGCTTTGCTCTTGTTGGGTTCATGATGGTGGGTCCTGGTGGCTTTTCAGAATTAGGGGACTGGGACATCAGCAAATTATCAGCCATCTAAGCATCAGACACTTTCATCTGCATGATGTTTATTTAGCAGAGGGATGCACACACATGCTATGGTTTGAGTAGTGGTCCAAGGACAACTTAAAGGAAACTAAGTTGATCCCAGGGATTCACCTCAGGTCACCAGATCTGGTGGAAACTCCTTTACCTGTGGAGCCCCAACAGCTCTAAAACACTCTTAGACAGTCAAGTTCCATGGAGAAAACCGCAAACATTTAAAGTTCTTTCCCCTTCACAAGCACTCTACAGAGAGGCTGAAGAGATGCATCAGCAGTTAAAAGCACCAGCTGCCCTTCTAGAGGACCTGAGTTCAATTCCTAGCACCTGTATGGTGGCTCATAGCCATTCATAACTCCAGCTCCAGGGGAATCCGGTGTCTGCTTCTGACCTCCATGGGCACAAGCAACACATGTGGCAGACAGACATATATACCAGCAAAGCACTCATACACACAAAAATAAAATAAATCTTAAAAAGTAAGAAAAGAACTCTTAGGACAGAGCAACCTGTGCATAACTGTGTGTGGGAGACTCCTGTTTTAGAAGGAAACATTTGAAGACACCAGAAGTAGGAAGATGGAGAGGATGTAGGCAGCCTACCAGGATTAGCTAACCTAGCTTTGTCAGAGAAACCAACAAGAACAGATTTTTTTGGTCTGTGGGGTCATCTTTAATCTTAGCACGTGGGAGGCAGAGGCAAGTTGGAACTCTGTGAAATCTCATTTCAGGCCAACCAGAATGACCCAGTAAGCCCCTGTCTCGAAATAAACCACCAACAAATCAATCAATCAGTCAGTCAGTCAGTCAACCAATCAGTCAATATTTTCCAAGAGTAGAGTGGATAAGAATTTACCATAAGCATAACCAGCAGTCACTGCATGTGTCCCCAAGTGTCTTCATAGCAATGTTAGGAGGCTGGGTCCTCGGGTAAGATCTACTTCCTTTTGTTGTATAATCTTGTGCCTCAGTTTCTTCACTTTCAAGTACCGCCCACCTCTCCCTGGGATAGTGGCCAGTGTGCAGTGAGGACTCAACCAGTGACTGATGCCTAAAATTAGCTCTGCACAGTGAGTTCCCTTATTCTCAGAGAAGGGTTAGTAATTTGGCCACTCTAAGGAGTTTAGTACATATATCAAGATATTTATGTCATCAAGGACTACGTGGCTGCAGTCACTCCCTAAGAGCTATACATTCCTCAAAGTCACACTCATTAGCCAAAGCAGGGGTCTTTGGTAGTATTCTAAGTAGCCCGAGGAAAGATGAGCAGGAACTTCCTGGGAGACAGGGTCAGAGTTTTCCTGGGAATCGCAGCTCTAGGCTTTTGTCCATTTTACCTACAAATCAAAAACCTGTAACTCAGGAGTGTTGGATGGCAAATCTAAGCACCAGGAACAGGACAAACCCTAGCTATTAGTTGTGAAGGCCTCCAGCATGGATCTTGCAACTTTGGCTAAACGCTTAGCTGGTCCCTACCCTTTCTGTAAGTCTGTACACTACATCTGGGGAGGACTCATCCACTCTGGCTGCAGAAGGAAGCCCCCTGCCTTGGGTAAAGTCCTAGAGAACATGTGTACACTCCCCTTTCTTGGGGAACAATCTGCTTAGCAACTTGAGTGTGTGCAAATGCACATAAGACTTTTGCGCGTGGCAGGTGTAGGACACAATTCTAAGCATGCTTTTGCCGAGGCCCATTCTTTGGGTAGACGTTTGGTGTTGATCACTAGTATTCTCCAGGCCCCCTTTGCCCTCTTGGATACTTGTCCCAAGATACAGCAGTGTGAAGATCTGACTGTGCACAGCCTGCGTGCTGAAAATCTGTGAGGCACTCTTCAGCAGTCGTGTTCCTGGAAAAGCCTTTAGTTTTTTGTAAAAATATGTAATCAAAGACCTCAGATTATCTGAGTGTGGTTGCTCATACCTGTAGTTCCAGCATTGGGGAGGCTACATACTGACTTCTGGGTAAGCCTGGGCTACAGACCAAGATCCTATGCCAAAACCCCAAACAAGCTAGATTTTCATTAAACACTTCAAAAACTGTGTTAGCTGGAGTGTTTGGGAGGAAGTTGCCAGGGTGTCTGAAGTACTCTTGTCAAGCAGACCAATCCTTTGCAGTGTGAATTGTGACTCCACCCACCCACCCACCCATCCACCCACGCACCTATCTGTTCATCCATGTACTCGCCCACCCATCACCTAAGTCAAGAATTGAGGCATTAACACAAACCTTTACTGAAACGCCTACTCACTGGCCAAGTGTGCACTGAGTTCTCAAAAGATAAATAAGGCAACTCCTCGTCTCATACAGCAGCATCCTGAAGCTCCAGCAACTGCGGTCAGGCTCCACATGAAAAGAAAAGGCTGCAAGGAGAAAACTAAAACCTGCCTTAAAGGGAGACAGAAAGCCAGAAGAGTGAAAAGCAGGAGAGAACTCTTCCAAAGGTGGCTAGACCTGCGGGAATCTGCAGGAGACGTCCCTTGGTTTCTCTCATAACTCAGTTTTTTTGTTTTTTGTTTTTTTTAAGTGCTGTTTTCTAAGGGTGGTAGGGAATATTAGGGCCTAGGCTTTTAAATTCTTAGCATAGATTCTGTGTTTTCTTCAGGTACTTCTGTAGGCTCGAGGATGCCCCATAGAGGGCGCTGTTTGCCTGGCTTTTAAGGCAGCACGGTGCTGGCAAGTGGAGGGAACAAGACTTTCTGGAGAACAAGTAAATTGCTATCCAGGCACCCGAGCTTTTTCGAACCAGAAAGGAAAGCTCAAGTCAGTGCTTCCTGATGTTGACGTCCAGCTGGAGATGCTGACACCCAGTAAAGAGACCCTTTCCTCTAGTGAATACACACACACACACACACACACACACACACACACACATGCTCACATTAGGAAAGATACATGCTGGATCGGCCCAAGTAAGCTGCCTCTTAACTCTTCTTGGGTCCACACTTCTGGGTCTAGTCCTAGAACTGAGATCGGCCAGAAGCCGTGGACCGAATGTCTTTCACGCCTGTCTTCCTGGGACAGATATGTCTTTCAGGTCTACGGACTCATAGATGGGTTTGTTGCGGGTTTTGGTTTTGGTGTGTTTGTTTGTTTGTTTTTGTTTTGTTTTGAGACACAGTGATTGAACACACGGCCTCTCTGGGTAGGCAGAGAGTAAGGTAGCACTGACTCACAACGACCACCTTTCCGAGAAGTGGAAAGTGTTTCCTCTTTCCCTAATTTAAAAGAGTCCTTGTACATCAGAACTTTAGCAAGCGTGCACACGTCTGCAGCTTCTCCCTCACCAGACCCGCACATACCCTTGGGAGCCTCCTCAGCCTAGTGGGCCGCCAGGTTTGCTGTGCGCAAGCAGTGAGCTCCGAACCGATGTCGTCTATTCAAGAAGAACGATTTCTGCTTTGGGTGGGTGGGTGGGGGTGGCCGCTGCTTGTCCCACACTGTTTGGGCGTCTTAGGTACAGCCTCCTCTCTGGACACCTTTTCTCTATAAAATCTGAGCGTATTACCAACCCTGCTTGAGCCTCGGAGCTGTGATTGGCTCCAACTCGGATTTTAGATGATGCGCCCCTGTGCATTCCAGTAAAGCCTTATACGAAATCAAAGGAATAATAGTAACCATTTATAGAAGCGCTACTTACAAGGCAATAGTAGGGTTCGTTACCGAGGGTGGATGTCAAAACTTTAAATACGACCTTCAAGACTTGGTAGAGGCGGTTCTGGTGCCTTAGGTCTGAAAAATAAAATAAACCTACTTCTCATTTGGGGACCCAAAACTGTCCAGGCTGCGCTAGGTAAGCAAGAGGAGCTTGAATTAGGCAAGACTCAACCGAAAGATAAAGAACGGGCTGAGGCGGAGGAAGTTCAGGTGTGAAGGGATTTTCATCCAGGCTGAGAACAAATTGTCATCTTTCTCGGGCCAAGGAACTATCACCAGGGGAAAGGACCCCATGGAAATCTAGAATTGAACTGATTGTCTCTCCGGCTTCCTGCTTGACCCAGGGAGCTGTTAAGGTCGAATGAGTCCAGCTATTCCTCAGAGGCCCGCCGAGATTCTCCAGGGACTTGGAGGCCCTTGGGACATGCTGCAGGTGTCGTTGCCAGGGTCACAAACGAGAAAGGTACTCTTCTGCGCCTTCGGTTCTTGGCGCCTATATTAAAGCATTAGGCAGTTCCAAATGCCACCCTTTGGGGGGAAAGTGCTGTGTTCAGATCGCAGGCTTAAAGTTGATCTTTGAGCCGCGGACACAGTCACCAAGAAAAGAGCGGTGTCCTTCGGAGTACAGTTGTCAGTGAGGAAACACCGCTACCCCGACAGTTGCACAGGGGAAGCGCGTGCACAGAGTCGCATACCCAACTCCTACTCCTGAAAGATTCAGAATCACTCATCCATTTTCAGGACCAAAGGCCAAGGCTACGCGGATTCCTAAGTCGGCAGACTCTCGACTAGACGAAGAGCGGGGAAGCTCTTTTTGGCCGCACCCTGATTAGCAGGTTTGGGGAAAGAAAGTACTGAGTTCTTTCGGGGCCGGGGAGGCGCCCTACTTCCTGGACCCGCACGGCCAAGGCTTTCCAGCCGCGTGGGGCAGCTGGCCTCTGCCAGGCCGGGAGGGGTGGGAGAGCGGCCGCTGCTAGCGCGGATAACCCGGAGGTGACCCAGGCCAGCCTGCTCTGCCCCTCCCCCTCAGGCAGACCGGACAGCAGCCAGCCCGCAGCCGAGCTGCAGGGGGTAGAGGGAGGTGCTTGCTCCAGACTGGAGCAGGTCCGTGCGCTCCGGCCCGCCGGGGGCGTCTCCTTCTTGAGGTCCCTCGCGCGCCTCCCGCTTGGTGGGAGCTCTCAGAGTGCCTCTTTAGCGAGAGCCAATATCCTTTTGTGCTAATAGGTTTGTCCTCATTTGCTGCCTAATTGGACTATATAAAGCCAATAACAGGCGGGCTCTCATAGCCCGAAGCCAAAGCGCCAAAATCCGAGGGAAGGCGAGGAGGGGGCGGTAGAGGAGGCGGCTGCGGGGCCTGGGCTCGGCTGCGCCTTGGCCGGCCTAATCCCATTTGCCATTGTACGCGCCCAATCGCCGTATACTCCCCGCATTTAACTTGGATGACATTTTGATTTCATCATTAGCATCCGGCGCCGGATTGACGCATCCCCAAGCCGGGGACCGCTCTCGCAGCCTCCTCCCGGGGAGCCGCAGCTCCTACCGGCCCCCTCGCTCCCTCGGCGCAGAGGCAGGTCCGCTCCCCGCCCCTCTGGACCCCTCCCCCCATGCGAGCCCGCCCCGGCCCGGTCCCCACCCCGCCCTCCCGCGGCTCGATTCGCGCCCGGGGCGGCCCCCGACTTTGAGCCCCGTAGTTGAGTTCCGTTTATGGTCTGATTTCCGGCCCCTAGCCTGCTCGCCCCGCCGCCCGCCGCCCGCCTGTCCCGCTCCCGCCCTCCGTGGGACCCGGAGGACTGGGGACCATGCCGGAGCCCGGGCCGGACGCCTCGGGCACTGCCAGCGCGCCGCCCCCGCAGCCACCGCCCCAACCTCCCGCGCCCAAGGAATCCCCGTTCTCCATCAGGAACCTGCTCAACGGAGACCATCACCGGCCGCCCCCGAAGCCTCAGCCGCCCCCACGAACGCTCTTTGCTCCGGCCTCGGCTGCTGCCGCCGCCGCCGCCGCTGCGGCCGCCGCAGCCAAAGGTGCCCTGGAGGGCGCCGCGGGCTTCGCGCTCTCGCAGGTGGGCGACCTGGCTTTCCCCCGCTTTGAGATCCCAGCGCAGAGGTTTGCCCTGCCCGCGCACTACCTGGAGCGCTCCCCGGCCTGGTGGTACCCCTACACCCTGACCCCCGCCGGCGGTCACCTCCCGCGACCAGAAGGTACCGACCTCTCTTTGAACTTTCGTTCTCCCCTCTTTCCCTGTGGGCCGGTCACATTCCCTCTCAGAGATGGTGTCACTGCAGTTAGGGATGCCCCGACACCTGCCAACCAGAGTTTTGGTTAACTTCCTTCGGCCTCTAGCCAGCATCTTTCCTGCGCCGCTCGGCTACGAGGCCAAGGAAACCAATCCCACTTGCTGAGAAAAGAAGTGCAGTTGGAGGCCCGGGCTCTGCGATTCCCGGAAAAAAATTGTCCTCCAGCAGGAATGGGTGGCTTGGGATGGCCTTGGGGGGTGG
D3_15 138688751 138703801 15051 TCACTTGGTCTGTCTGTGGCAGTCAACTCCATTTATCTCCGTTTGGACTGAAGAATTCACTAAACAAAATGTTTAAAGGGAAAACACAAAAGCCTCAAATCCAGACCTGTAACAGCTTCATATCTGGCTGAGTGGCAGTGGTCTTACTTATTCCTCGAATTTATTTAGTGGTAATATCACTATTACTACTTTGCCTGCTTCAGAAGTTGGTTTCCTCTCTCACGCTCTCTCTCTCTCTCTCTCTCTCTCTCACTTTCTTTTTTGTAGAAAAAAATACATTTTATCCTCTCCCGGTTTTTTGTTTTTTTGTTTTTACTTTACAACAACCGTTGTGCTTTGGCCTGTGCAATATTGTACAAAATTTCCCTCAAGCCCCGCCCCTTCCTCTGCTGGGATAAAGACCACCACACGGCAGCATAATCATTAATGTAGAGGGAAGATTAAGCAGGAGAGGGGGAAGGAAGAAAGCAAGGTAGGTAGCGAGGAGTGAGCTAGGCAGAGTGGAGCGCTTTAAGTGAAGGACAATCAACGTAGGAGAATCTTAACTTATTTGATAACTGTACAGTTTTCTTGTAAAACCTCCCAACTCAACCGGGCACCTTGCTCTGGTTCCCTGTTGGCTACACCTGTTGTTCTGTTGTTTTCATTCGTCTGTTTCCCCCCTCCCTCCCTCCCTGTCCTCTGCCGGAGTTCAGTCCCTTTATAAGAAAACAAAACAAAACTCCACAAAATATTGTTACACTTAATGTTATTGTGGAATCGAATTAATTAAAAAGAAAAAAATAAGCCTTTCATGTTGCCAGAGTGTGTAACTTTGGATGCTTTCCACGCCCCTGGGAGCAGGTGAGCAGGGAAACTCTCCTTGCCTAGCTCTCTGACTACCTCTTGTGCTCTCTGAGTCTGACCTGCACTCACGCCCCTACAATCCAGCCCGCAGAGGAGACAGGGGCGGCTTTGTTCAGGACCAAGGGCGTTCTTTCCTCTGTATCTTCTGCTGCTTGAAAGGCCCTCGTCAGAGCAAGGAAGAATGAGGCATGGGAAAGGTATAAAGATCCAGGTTTATGTCCAGCTTGCAAATTTGAGCAAAATAACCCTGAGCTTAAAGGCAGGTGGTATTTCCCAAGAAAAAGCTAAAGCTTGCATTCTTTCTTCAAGCCAGGGTTCCTTCAAGAGCTGGACAGGACATCCCTAAGATGTCTGTCTAAGATTGTGGTGCCTGTGATTGTCCGAAGGGGTGTTGGGGAAAAGAAGGGAGCACAAAGCTTGGAATTCTGCTGAATCTTGAGGCAGGTCATTAAACCTAGCAGTCCTTGAGATAGATGGACAAGGAGTTACCACTTCTAGGACAGGGAGACAGCTGAGCTGGGAGAAATTCAAGCATCCAGTGTAGACCTTGTAGAGGATGTGCCACTGGCTGAGAAAGGACTGGAGTTGACTGGTTCCCCCAACCAGGCTTTCTATGCCCATCGGTTCTCAAAGCTGCCCCAACAGATAGCTAGCCTGTCTCCACCCACCTGTTTTAGCCAGGGCTTAGAAGCCTTTTTTCCTTAGGCTACTTCACCCTTGGTTCTCAGCATCAGAGGCAAGGCATTCCAAGACAGCTTAGAACTGGTCTTCTAAGGACCGAGGGAAGTAGGTGTTCAGATGCCCCTTGCCAATTCAACATGCATGCCTGCCTTCAAGCTCTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTCTGCTCATACTCCAGGCCCTGTTCTTTTGGAGCTGAAGAACTAAGCAAGCAATGGCCGAGATTTCAGAGAGCATTGCCATACGCAGGACACAACCACTGGGGAGCGCCTGTACATGCGCTCTTTTCTCCTACTGTGCTGCCAGGCTCCTCCAACTCAATCGCCACAATTGATTCTACTGTGGCTGAAACGCAACTATCGTTTTTTTTTTTTTTTTCTGTTGATGGAAAACTGGCCCCAAATAAACCAGTGTGGGTATTTGTGGCAAAATTTTAGCCTGGACCTCAGTGAAGATAGATCTGCCTTTATTTTCCTCTGGTTTGGAATAAAAACGAGGGCATGTGTCGTAAATGAAAAATAAACAGAATATCAGGGTCAAAATATGTTGTTCCCTGTGTTGCCTGAAAACAGAGTGATGCCTTTTACTGTTGGCAATACTAACTGCGGGACAGGCCAGTAACAGTTCATTAAAAATAATGTTGCAATAGCAAACATGTCCTCAGATTTTACAGCCAGAGCATGATGTTATCAAATGCCAAGAAAATACTTCTTTATTTGTTTGGATTCCAGAAAACGGTTCTTGACTGACCCAGTCTTTTGAAAGTCAAGATGTAACTGGTTCTCTACAAGCGTAGTTTATCCTTTGTGGGAGGCCCCTTGGCTTCACTTTTCTTCCTGCGTGGGAGCTTTGCTGCTCTGGGCTGGTACCTCTCACCCCAGGGCCACAGTGCAGCTCTGGACGATGATGGCCGGTGTTCATACCATCTTCCAGCCCAGTGGGGTCCTGATGGTTTTCTCCCATGTTCAGTGTTTGAGGAGGAAGCCTGTGACATTATGGAATACTGGTGCTGCTCTGAGTGGGGCACAGTGGACCTTGCTGTCAGAAGAAACCCAGTACAGGACCAGAGAGCTGGTTGTCTTCTCCTGTGTAGTCTTTGAAAAGATTCCCAGGAGTTTCCAGAGCCAATTACCAAGAAGACTTGTAGGGAGAGTGACAAGTCCTCCTGAAATCCCTGTGGACGTTCCTTAGGAGAGTTCAGGGCAGCCAGGCTTTCTGTTTCAGGATGCTAAGTGGGCACCTCCTATTTTATCCTTGTACCACCTTCAGGTTGTGGAACCTGGAAACTTTCTCTCTGTCCTACCTACAGTCCTGCACCCAACCTCAACACCAGTCAGTGCTCTCTGGTCAATGAAACCTTCTACAGTGGCCACCAAATAGATGGATGTTCGCAAATAACACAGAATACACTCACTTGACACAGCACACATGGCGATCCAAAGTAGCTCAACACACCCTCTCACAGTTTGTGGTTTTTAGCATCATTCTGATCGCTTAGAGCATACACAGAGTCACGTGCTTTGCTCAGAGCGACTCCAGGTGGCTGGTTCTCTCCAATGGTCCCTGGTTAAACTGAATTGAGAATGGATAGCACAGATGGGCAGGAATAGGGCATCTACCCACCCATCTCAGCTGCATAACCTGAAAATTCCCCATGTGGCAGGTTGTTCCTGGAGTTTCATCACAAGCCACGTAGTCAAACTAGCCTAACCTCACCACCCCTCTACCAGGGACCCTCACAGTCCTGTGTGCCAAGACTATAAGGAGTCCCCACAGAGTTCCAAGCACCTCTGAGTGCCTTCAGGCCCTGGGAGAGCTCAGAGCCAGCCTGTGAACTGTTTCTGTTGGCAACACAGATGTATTTCTTTTATTTGGTAGTAAATAGAGTAGATGTGTGTACATCAAACAGCAGCAAGATATATAAATGGCAACCCGGCCACTGGGGCTCATTCCTAAGGGGGTATGTCCCCTCCGTAGGTCAAAGTCAGAGACCACAAGGAGGGAACCCCAGAGCACCCACCTCTCCTTGCCTGGGAAACTAGAAGTGGAGCTGGGGAGAAGGAGAAGGAGGTGGAAAGGTGGGTGAGATTTATGTCTTTTCTGAAGCCATCCTGTACTCGGCAACTGGGGAGCGGGAGAGAAGGGAGGTTCCAGGAGTCTCTAGGGCTGCTCAATCTGGAAGCTTCTGGGGCTGTAGCACTAGGGATGGAGCATCCTTTTGATTACAGTGTCAGTATCCCCGCTTCCGATCTCTCTCAGCCCCTGGGAGAGCTGAACTGCCTTCCAGAGTCCTGGGGTGGGGTGGGGTGGGGTGGGGTGGGGGGGTGGGGTGGGGTGGGGGGTGGGGTGGGGCCCTAGCCTTTACTGGATGGGCCCTTGACCACTTCAAAGACCTCATTTCATTTCACTTTAGCTTGCACTGTGACTGTCTCCTTTCCGTAGCCCAAACTCTAACTCTGCAAAGTGCCTAGGAAACTGAGCCAAGGCGTCAAGGGTGGTCAGATCCACAGAAATCCTGGAGGCCGCCAGGAAGACCCAGGGGACTCTGCTCACACAAGCATTTGCTTGACCTTAAAGGAGCCATCTGCCTAGCACTTCCTGTTTTTCTCCTGCTCACTCTCTACCAGCTACCGGGCAATGTTTACAGGCCCTCGCTGACTTTAGGGGACTCCACCAGCATGCACACATCTGAAAAGTATACCCGGTTGAGTACACACCGGGTCTTTCCTTGCTGGAGCAGATGATGTTGACCTTTTATCATACAAACAAGGAGGGTGAAAGAGGCACCTCTCTTAACTATGCCTTAAATATTAGGGAGCACTGCCTAGTGCTTCCCGCTGGGATCTAGGATGAAGATTTGAAGTCCACTTGATTGCTCTTCTTTTGTGAAAACTGTGAGAACCAACAGTGAGGTGGGAAGGGAAGGGGGTGCCAAGGAGGTAGGGTTCTAGACTACAACCATAGTCCCAGCCCGATCTATTGATCTGCATGAGACTCCTTCTAACTCCCACCCATGACTGGGCATGACTGGGGAGAATATTGATGCTTGATGCTTCTTGCCTCCAGGAAGGGAGTGCTCACCGGCCATGCTAGCTTACTGTTCCATGGAGAGGAGTGCAAAGTCTGGGCGCTGAGGTGGCAAAAGAGGTCTTTCTGGTTCTGGTGCCTGGAGAAGTGAGCCAAGGAAAAGGTGTAGGGGAAGAGAGATGCGCATCATTGAGTCGGGAGCAGTGAGGTGGACTCATCTGGTGTCCCTCACCCCAGCACTAAAGTCTTAAAGATATAGAAAGAATCCTCTCTCAAGAGAGTTCCTTCACCACCCTCACCCCCCTCGGTGATGGAGTACAAGCATCAGATTTTCATCCTAGTTCCATTAAGTGAAACATAGGTTGCAGGGCACCCTTTGATTGAAACAGTTTAAATTTTAATTGTGTTTTTAATTTGACATATAGTTGCAGAAAGGAATGACACTCAGTCGCCAAGGGGCGCTCGATTTTCTTAATTTCTCCCAGAGGAATTGATGAGTCTATCAGGCTACTAGATTGGAAACCCATTAAAGCTCTCTGGTTAGGCTTCTAATTGGAACTTGATGGATGTGGGAGGGGGGGGGGTGTCGGGACTAGGCTATGCTGATCCAATCTTCATGACTTTCTGTTTTCCAATAAATTACACAATTCATTTTCCAGCCACAGATTACATAAATTGTACACCTCTGGGGTTCTCTTTTTCAAGTAGGGAGCCCTTTTCCCCAAAAGCCTATTGTGAGATGTCATTTGCACCAAAGAACGAACAGCAGAGGCCCTTGAATGAAAATCGAAACATAATCAACGTTTATACTTAGAAAATTTATTGAGATCATTTTCTCTGGTATTTCCTTATTTTAAAGTTTGGACGCGCCATATATCATAATCCACTCGTGGAAAGGGGCGGGGGCGAGACTGTGTTTAATATTTATCGAAGCTTGATTCCAAGATCAAACTTGTTTATGACTTCATCTGTCATTTCGGAGGGAGCCTTCTTCCACTATTACCAGGCTCGGCCAGCCTATTTCCCAACTGCCGCAGAGCAGAGAGGATCAATTTTTATTAGGCTCCTCCGCAAGTTTGCTCTGGGCCGCTTTAATATTGAGCAGCCACATAATCTCGGGACCTAAACTTTAAGTGTTCTTTCAGTCTGGAGAGGTTTATCATTCCTTGGAACAGGGTTTGTGCCTAACCCTTATGGAGAGGAGACTCAAGCCCCCTCTGAAAAGCAACTTCCTAGGGTGTCCATTAAACAGAGAGACATAGGGAAGGGAGGAGCTATCTGGAGACTCAGTGGGCGGGGCCAAGTGGGGGAGTACTAAGCTGAAGGATTGATCAACTTGATCCAGAGAACTGTAACTGAAGGTTTCTTGGAGTGGGTACCAGAGCGGTGTGGGACTCAAAAGGCTCTGGCCCTTATCTACACACTTAGTGAAGTCTAAGTCCCCAGGAGTCTGAAAGAGGAGGTGCGGTGTTGCTATTTTATTCTTTCCAGTTCCTCCCTGTGACCGAGTAGAATGAGAGTAGAATGACTAAGTGGAATGAGTGGGATGAGAGGTGACCAGTCAGAGGAAGGTCTTTAAGCCTCTTGTACCCACCACCCCCACTCACTTAACTAGCTTAGTGTGTTCTCTGTGCTGGGTGTGAGAGTGTGTGTCCCTTTGTTTCTGTTGTGCGTGACTTGCTAGTTCAATGTGTATGGGTGAACAAGTGTTTAGATGAGAGTGCATGTGTCCTTTAATAGCTAGCTGGTCGGGGCTTGAAGCAAGCATCCCTCTGTGGACAACACCCTCTGAGCAGAGGAACAGAATAGGCAAAAGGCTGCCTTCAAAGTCCCAAATCAGCCCGGGAGTGAAGTTTGGTCACGAGGTCTCATAGGTTACTGGTCTTAGACTTGCATCTTCAGTAAAGTGCCCTGTTGGTCTTTGCTCTGAGACCTACCACATTAGTCACCTCTCATAGTCCCTGCCTTAAACCCTTTTAAGTGTTTGACCCCATTTTGAGCCCTGGAATCTCTGGGGGTTCTTTAACTCTTAAGGCTGTTGTTCTCCAAGTTTGTCGCTTTTGCCGGCTTCTCTTCCCATTCTCTCCTAAGAGCACAGTAGCGGCAGGGAAACAACCCTGACCGCAAGCTTGTTTCCATTACTTGGCTAAATTCTTATTTTTCAGCACCTCAGTTTCCACTTCTGTACAATGGCGGTAAAGATCTGAGTCACGTTTGCTGTGGTTAAAATGAGCTGTCCCCAACACGTTCACAGCCCCAGATCCTTTCTAGCTCAGAACGACTTAACTCTTTCTCCTCCACTATGATTTTCCTACAATCTCTGTAGCTGGCTCTGATCCTCAAATTGGCCAGACAGTCCTCTTGTGGTATTGATTTGGCTCTAGCTTCGACGACACGGCCCAGATAACTGTGCTTTTGAAAAGCCAAGGGTTAGTTCATGAGTTTGAACATTTCTTTCTGGAATCTCCTCCTCTACCTCCGCGGGAGCGCGCTGGACCTGCTGTGATGAGGCTTCGGAGCTTGTGCCAGGGCCGCGCAGAGCAGGACGCACAAATTGTATTTCAGCGCCGGGTCCTTCCGGGTTAATGAGCTGACACCATGATTAAAGCTGACCATTTGTAATGTGTCTCGACCCCGCCGCAGAGCCCTGAAGAGGTTAATGCGGTGACGGAGGCCGGCACCTGCCCCTCGCCAGCCTCCCGGGCCTGGGCGCGCAGCCTCCTGGCCCTCCGCTCCCTCCCCTGCCCCGTCCCGGCTGCGCCACCGACCCCTAATCAATTAGCCCATTAACGAGCCCTTTGAGGAGTTAAGTAGGGAGAGTTCTGCCACGGGCAGGGCCGCAGTCGGTAACTCAGTGAGGCTAATGATATTATAAGCGCTTTACTCAATAACCCGGGAGCGGTGCAGGCGAGGAACTGCACCGTCACGGTGCAGGCTGGACATTTGTGGGGACCGACTCTACAGTCTCTCTTCACTCTTGGATCTTCTGGCATGTGTCTAAGGAAGGGAACAGGGTTTCTGGGACCTTAAGGGTGCAGCCAGGTTTGGCTGGAAGCTTTGGGTCTCGTTCCTTTCCATAAAGCTTGTGTAGATAACTGTCGCCTCAAATCCCTTTGCTTGCTTTGTTGAGATGACTCTAGATCAGGTAACAGGTGCTCTAGGGATTAAACAAGCAAGAGTCAGGGCTTAGGGAGAGGCCACAAGAAGGAAGGAGAGAGTCAGGTTTTATAAAGCTGTACCTTGACCTCTGAAAGGAAAGCAACACTTCAAATAATTTTAGGATGGGGAAAACGAGGCGTGGAAAGTGTACATATCTTGCCCAAAGACACCCTACCTATATTTAACAGGATCCTGTCCAATTCTAAAAAGTCCCCATACCTACCTACTTCGTTTCCTTCCAGAAGCAGCGTAGCCATTTTGAGACCGCCAGTATTTACCAGCAAAATAAAGTAAATGAGTTTAGCTGCAGTGTGGGAAGGCGAGCTGTGTGGTATTCGGTCAAGGATTAGGAAATAACTATTCTGCACTCTGAGAGTTTATAGGTTTCTAGCTGACACATCATCACAACCAGAGAATGGGGGCAGGCGACACTAAGAAGTTTGCCAGGGGGCAGGGATGCTTCTACTGGGACCAGACTTGTTCAAGTTCACACAGTGCAAATGGCGTTTGCTGGAATGTGAGCCGGAACCTCGGGACATTTGGGGGCTACTCTGTTTCCAAAATCCTCTGCCCCCTTTAGTCAGCGGGGTCTTCAGGTCCAGAGCTGTAGAATTTCTTTGCTCCTGTGTCTCCCAGCGGAGAGCCACATACTGTCAATCCCCAACGCAGCGGGAGAAGACTCGTAGAGCGCTGTTAGTTCCCAAGAGGCTAGAAGGGGTTGAGGACTGGGGAGGGTGGCAGCAGCCTATGGTGTGCATCTTGGATGTGAGAGAGCCCCACCAGCTCGACCCCGGGCGCTGTTCTCGTTTCTGGAGAGGCAAGGCAAAGACCAGGAACACAGGCTGCAATCTAAGCTGCCTTCAGCCCCAGATGTTACGGGCTGGACAGAGCTGATGCGAGTGCGCGTCTAGGCTTGCTCCCGGAGCTTCCCGGTGCTGCGTGCTCGGCCCCAGCTTTTTAAAAAATTCCGGGGCCGCTCCGCGGCGCTGCGATTGGCCGCGGCCGGGTAACCTCTATGCAAATGAGGCCGCGCAGCCTCCGCGTCGCTGGAGACCGGTGGAGTTGTCAAACGAGGAAGGGACATTGGCACCGGGGCTGGGCGGGCTCACCCAGAGCCGGGCCGGCAGGGACCCGCGCACAGCGGTCCGGTGCCTGCGCGTCCTGGAGCAGAAGGGACGCCTCGCCAGCCTCGTAGCCCCCTCCCCTTGCTTTCCCTCCCGCCCCTCCCCCCGCCCGCCTGCCCGCACCACCTTCCACCCAGCCCCGCTCTAAAGGGAGTTCTTGGTTTCCTCCGATTTCTTATGAACCCAGGATGGGCAGCAAGGAAGATGTGGGGAAGGGATGTCCGGCGGCCGGTGGCGTCTCCAGCTTCACCATCCAGTCCATCCTGGGCGGGGGCCCCTCCGAGGCACCGCGGGAGCCCGCCGGGTGGCCAGCCAGGAAACGCAGCCTGTCTGTGTCCTCGGAGGAGGAGGAGCCGGAGGAAGGTTGGAAGGCTCCGGCTTGCTTCTGCCCAGATCCGCACGGCCCCAAGGAGCCAAGCCCTAAGCACCATACCCCCATCCCTTTCCCTTGCCTGGGTAAGGATCGCAGGTCGGGAGTCTGGCCCGAGTGAGCGTGAGCCGATGCGGTGTGTGATTGTCGGAGACGCGGAGCAGGGCATCCCCGGGTCACAGCCGGGCCGCTAGTGGTCCAGGCTAAGGGGAGGGGACTACGCACCCATGGTGCATTCTCGAAAAGGCCGGAAGGGCCGGCGCTGGGGAGAAGTAGAGGAATATTGGGGCGGTAAGGGGAAGACTGGGTAAGGGGAAGGAGGGTGAACCAGAGTGGGCCAGGAACTCTGCGGGCGGTACACAATGCTGGCCAAAATGGTTTCGCACGCCGGGTACTTTCTGTCCTGACTTAGTTCTGCAGGTCTGGTTTGGGTGTTCTGTCAGTCCTGCAGGCTCTATGTCTTTTCCCTCCTCTCGGCCTCTTCTCGAAGTGATTAGTACTCTAAAATGATTGACAGTGCTGAGTTTCAGAGAGGTAAAAGCTAACGGAGGACAGCCAAGGAGTTATGAATAAAATTAGCCCGACTCTCAAAGAAAACGGCCTGTAAAATGATGCTACCTTGTAGAGCTCAGTCCTCAAAGGCAAAAATCACCTTTAAAAAATGGTGTTAATGTCCCCATTCCAGACCCGGTACCACGTTTACTTTTATAAGAGTGGAAGGAGACCCTCCAGCTCGGATTATATGGAACCCTCTTCTGCTGCTTTGAAAGGCCGAGGCTCTGGACTTTGGGCGCTAGCAGGACGCAGTAGAAAAGGAAAGGGTTAGCAATAGGGGAATCAAGGATGCAGGCATCAGTCAACCTCCTTGTCCCCGGCAGAGCTGAGAGTCTTTAAGGCTCATCAACTCCATGGTCATTCTGTTTGTGCTCACTCTTCAGGTACCCCCAAGGGCAGCGGAGGCGCAGGGCCTGCGGCCTCGGAGCGCACGCCATTCCTTTCTCCTTCTCACCCGGACTTTAAGGAAGAGAAGGAGAGGCTTTTGCCGGCGGGCTCGCCTTCTCCGGGCCCGGAGCGGCCACGGGACGGCGGTGCGGAGCGCCAAACCGGAGCGGCCAAGAAGAAGACGCGCACCGTCTTCTCCCGCAGCCAAGTGTACCAGCTCGAGTCTACCTTCGACATGAAACGCTACCTGAGCAGCTCGGAGCGCGCTTGCCTCGCCTCCAGCCTGCAGCTCACCGAGACCCAGGTTAAGACTTGGTTCCAGAATCGCCGCAACAAGTGGAAGCGGCAGCTCTCAGCCGAGCTTGAGGCAGCCAACATGGCACACGCGTCGGCGCAGACTCTCGTGGGCATGCCGCTCGTGTTTCGGGACAGTTCGCTGCTGCGAGTGCCGGTGCCGCGCTCTCTGGCCTTCCCGGCTCCGCTTTATTACCCGAGCAGCAATCTCTCGGCCTTACCGCTCTACAACCTGTACAACAAGCTTGACTACTGACATGCTCGCCCGCCGGCTCCCGGGACGCCCCTGGTCGCCTGCAGCTGCCTGCATAGCCCGGCGCGGTCGGGATCCTTCGCAGAGCTCCGGAGCAGAGCGGCGCGTGTCCAGAAATATTAAGAAATACACCATGTGTATTCGTTATGTCTTATTTATGGCCTCTTTCCTACCTTTTGTTTTGTTTTGTTTTATTTTGTTTTGTTTTGTTTTGAGTATTTATCTGCATTCCTCACGCCAGAGCACCTGCTTTCTACACAAAACCAAACAACTCGCGTTTGAAACTATTTTGGAAGGGGAGGCTCTCGGGTGGTGGTGGGAAGTGGGGTTTTGATTAGGTCGGTTTTGACCAGTAGCAAGAAAAAAAAAAAAACATCAGAAAAACCACCAACAGAACCAGGCAGTCTTTCTCCCATATATACACCTGTATATAGAAAATGTGGTAGGACACAAGCTCAGCCGAGCCGAACCAACTAGGGCTTTGGTCGTTGAACTAATCACTTCTCTTTATTCCCTTCATCTTGAGGATGGGTAGGGGCAAAACGAGGAGAGTACAGATCTGTAAATATTTTTAAAGAGAAAAAAATAAAACGTTTTAAACATGGTTGCTAACTTTGGAGGGATGGATTGTCCCAGGAGACAGCGGCAGAGGAGTTTCTGGATTTTTTTTTTTTTTTTTGCCAGAGCATGCAGGATTCTGTCTCCTCCCTCAGCCCCCAAGGTTCTGCTCTCACCACGTGTGGGTGGGGCTGGGCTCAGAGGTTAAAGGTCGGGATATTGGGGCTGCCCAGGCAGCACGTGACTGGGGCTGCTTACAGGAACAGCACAGTTCCCACGACCCAGGTGTGCGGGTTGGGGTGCACTCGAGATGGAATCCAGATCCACAGCCCCGCGCAGCGAAAGACAAAGCCAGGAGCCTGGCTGCTGACCCGGTCGCCTCTGGGAATGAGCCAAGCAAGGAGGTGAACATCTCCCCGATACTGGGCTGTTTTGGGCTAGACGAAAGCTGGGCACGCCGCGAAAGGCCGGAGCTCTTGGCGTTCTTGGCGTCCTCCTGCGCGGAGTAAAACGTGGGGTGAGAGAAAGCGTCCGCTTGGGTTCCAGAGCCACAGGCGACTGGCTTGCAGCCCTCGCAGGGGTCTCCCGCTGGTGACACAGTTGCGAGAGTTCTGCGCAGAGCGGGAAGTGATGCCTAAGGGTCAGATTTCGTTGGAGGGATTCGTGGAGCAGATGACACGGGCGCTTCCCTAGTTTCTTTTTGTAGTTGTTAAATTTGCCCGTGAATGACAAAAAGGTCGGATCACTGGCTAACTTGCAGCAGCTGTTTCTGAATTGAGCACTGAGGATTGCAGTGCCCGGCTGCCTGCCTCCATGCAGGGTTAGGGAGGCTCTGCACCCGGAGAACTGCTTCGTCAGGATCATGCAAATCACAGCACTGTATTTTCTCTACGTTTTCATAGCATCCACCTGTTCCTTGTTAAATGTCAGAAATGGGCCTGGAGCTTGGAGGAGGCCTCAGGTAGAGTTATTAGGTCCAGCCCGCCTCCCCTCAGTGGTTGATCAGGGTGCAAAGAGCTAGGAGTCTGCAGCGCCAAGGCCCTATCTATTTCTTTCTTCAGAAGAAAACCTGGTGCCTGAGGGACCGGTCTCTTTGAGCCAAGGGGGCATCGAAATGACTTGCATTTTGTAGAAATTCACAGAATGTAAACGTTTACATGGCCCATATCATTCCGTGAAATATGCCTGGCAGAAGAGATTTGCTCTGATATTGCCTATTTTTTTCTTAACACATTATATTTTATAAAACCTGCAACTCCTCCTAGGTGACATTAGATGGATGCTACACAAGAATGCACCGGGCAATACTAGGTTGGTTTGCTTACATTTGTTTTATTTTAGCTGTGTTCACCTCAATAAGTATTTGTTGAGGTCTCAACATGTGCTAAGAACCTACACTAAATTTGGGGTGGTCCAAAGGAGAACACAAAGAGGCCTATCAGTTCTCTTCTTACTTATTAAATATGTGTTTTCAATTGCTCATTGGATATGTGTATTTTTCTGTCACCGTAGTTTTCACTCCAGATAACCTCTCTACATTCTTTCCCTGACAGCATTCTTTGAGTGTTCCACCCAGAAACAACCACATTACTTCAGAGGACTGTCTTGTAAAAAAACATACTCCGTTCGGATGCTTTTGATCTGCCTTTCAGCAGATTACAATTCGGTGATGGATTTAACAGTATTGGGTCATCATATTTGCTTCAGGATGGCTTTCTCCATAGGTAGTGACCTCCTCCTCCTGTCACTGTCGGCATTAACGACTTGAGTTTGCCCATAATATTTTAGGGAATGCCATTTCCCGCAGTTGTCATGTGGTATATATCTGACTCTCTTTACTCACCCAGACATTAGCTGTGGCAGGCGACTCACCACTGCAACAGGTAACTGAATATCACACACACACACACACACACACACACACACACATACTTACTTTACATTTGTGTTGTAAAATGGTCAAGACTCTGCTTGTGGCCAGATTTCCTATCTCAAGTCTAGCAATTCATTTGAATAATCAGCAAAATAGTCCATCTCTTTTCAGGCTCTAAACTTGATTTACAAGATTGACGTTTCTTAAAACTCAAATAGAGTTGGGCTGCAAGTGTGCGGCCAGAGGGGCCTGAATCATACACACATGGGATAATGAAATATGCTTAGATTTTATTAGCAGGTAATTTATGCTCAGAATCTGGAAACAGTAATAGAATTAAATAAACAACTGGGAAAGAGAACCAAATAAGTCAAGAGGTAAAATTATTTGGCTAAACTTAGAATGCAAAACAACTCAATGCATTAAAAAAAATTGAACTTGGCCATTGATTTGTTGAAAGAGAAGAGAGAGAAAGAACCACATACTAGAACTATTTGGAGAGGGAAGACAACCAGAATATTTTAGCCATAATAACCATCATCAGGACAAAAGACTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTATTGTCAGGAATCTTAAGTGCATTTAGTTCAGAGTCTGTGCACACATAAGATCTTTCTTTCTTTCTTTCTTTCTTTCTTTACTTTTATTTTTTTAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCACCTGCCTCTGCATCCCAAGTGCTGGCTGCGCCACCACTGCCCGGCAAGATCATTTTTCTTTTCTTTTCTTTTCTTTTTTTTTAAAAATGTACCTTGAAAGTTAAGTAGTTTAAAACTAGAGACAACTTCCTACTTCATGCAACAATTTCAAAATAAAAGGGATTGTCTCAAAAAGATGGGACAGAGAAAAAGAAGTTGTATCCAAGGATTAATTAGAAACTCAGTAACATTTTGCATGTCAGTATGCTACTATGTTGTTTGTTAAGTACTGCTTTTCTCTCACAGAACCAAAACAACCTTTTGATTCTCATATTTTTTAGAGGATTCTAGAAACAGAAAGTGCAGCAAACTGAGCCTGCAACTTCCATGATAATGCAGAGGAAGGTGGGAGAGGGAGGACAGTCACAATGCTAGGTCCTATGATGAACATTCCTAAGAGATTCTGGCTGATCGTTAAAGATTGGAAATATTTGTGTCCTGCTCTGTTCACCCTTATGGGTGTGGTACTCTTCTAAAGCAAGGAGTAGTTTGACTGAGTGTGTATTCCCAGAGCTGCTTAAGAGGCCTGACATTCAGGTGCTTGAAAACTGTGGAGAGATGAAACGATAAAAATGAGATCACTAGAACTCAAGGGAAATGAGTCTCTAGTTCACAAATTGATAACCCAGCAAAATAAAAGCAAATAGTACCTTTGACTAGAAATTATTCTTCAGTATGACTAGTCTGGATTAAGTCCAGGGGTATGTGCACGGCTAGAAAGAAAATATTAATTTATATCTATAGTAGTTATCAACCAAAATGAAATTCTGTGTCTGAGAGATAGAGGAGAAGGATGTCACAAGAGCTAGATAAAATCTCTTTTCTGCCTTCTAGTCTGTGGATGTTCAGGCAAGCAGCTCCAGGGAGACTACAACTCCCAGCATGCAGCATACAATTGTGGGCGGGCGTGAACGCCTTCATGACCTGAGCATACTTGGGAGTTGTAGTTTTCCCTCCGCAGCTCCAGAGATGTAACCTCGGCGTGGAGGTACTAAGAGACCCGGTGAACGGGGGGGGGGGGGGAAACGCGGCTTCCCTTTGAGCCGGAACAGGATGACTGCGGAAAATGAGGCAAGCAGT
GF3 -1 -1 1 CATTGTGATGAAGTTCTTGGCACTTGGTAG
GF4 -1 -1 1 GTAAGACTGCAAGCTCGATGCAGCATC
EX52 -1 -1 1 CAGGGGGGAACGACAAAATTACCAGGTC
EX32 -1 -1 1 GACCTGGTAATTTTGTCGTTCCCCCCTG
GR3 -1 -1 1 GTGGATTATGATATATGGCGCGTC
GR4 -1 -1 1 GTCATGAAGATTGGATCAGCATAGCCTAG
Comments:
Created
Target: 2803Ensembl ENSMUSG00000040148 -- 138687225 -- 138688700 ENSMUSE00000385956 -- ENSMUSE00000385956
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: 7, start 138687225: end 138688700
Found 2 RP24 black6 bacs on NCBIM36
Found less than four RP24 bacs: looking for extra from RP23
Found 5 RP23 black6 bacs on NCBIM36
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:7:138680725:138681724_776 G3:7:138692200:138693199_855
REJECT (1) gap pair G5:7:138680725:138681724_776 G3:7:138692200:138693199_869
ACCEPT (0) gap pair G5:7:138680725:138681724_776 G3:7:138692200:138693199_842
REJECT (1) gap pair G5:7:138680725:138681724_903 G3:7:138692200:138693199_855
REJECT (1) gap pair G5:7:138680725:138681724_903 G3:7:138692200:138693199_869
ACCEPT (0) gap pair G5:7:138680725:138681724_903 G3:7:138692200:138693199_842
REJECT (1) gap pair G5:7:138680725:138681724_898 G3:7:138692200:138693199_855
REJECT (1) gap pair G5:7:138680725:138681724_898 G3:7:138692200:138693199_869
ACCEPT (0) gap pair G5:7:138680725:138681724_898 G3:7:138692200:138693199_842
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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