High Throughput Gene Targeting: Create Design

This custom vector design tool is provided by the EUCOMM consortium as a service to the research community. When using this tool, the user agrees not to submit more than five designs per day for creation without prior discussion with the High Throughput Gene Targeting Group at the Sanger Institute.

Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by mk2
Valid After Annotation
yes
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 96830
Chr: 17
Strand: 1
Status: Ordered
BACs: RP24-105H4 : RP24-374K9 : RP24-510G2 : RP24-222D18 : RP24-574N12 : RP24-232L23 : RP24-88H3 : RP24-87H23 : RP24-135P19 : RP24-86G12 :
Instance: Plate 101: Well B01: BACs RP24-88H3 RP24-87H23 RP24-232L23 RP24-135P19
Oligos on GRCm38:
Type Start End Length Seq
G5 8430453 8430502 50 GAACTCCTGTACAAATGACCTGTATACAACCACTCACTAAATGAGCGCCT
U5 8435672 8435721 50 AAGAAGGCCTGCCGCTTGGGAACTGGCTCTGGCCATAGGTGAGCATTGGT
U3 8435784 8435833 50 ATTCCACCCTTCTGAAAAGTCTTTAATTGGGGCCTGTTTCCAAGAAAACT
D5 8437374 8437423 50 TAACCATCTTGGGCTTTGGACACTGGCAGGCAAAAGAACTTTTGATTTGT
D3 8437450 8437499 50 AGTGGACTCCTGGGAGTTTAGACTTGGGTATGGTGAGGGTTCGGCTATCC
G3 8441476 8441525 50 TCATAGTGAAAAAAAAATAGTCTGCAAAGCCCTGTGATGCAATTAAGTAC
Oligos:
Type Start End Length Seq
D5_D3 8630289 8630314 26 TTGTTTTTCTGCAGCAGCCAGGGTGG
G5 8623318 8623367 50 GAACTCCTGTACAAATGACCTGTATACAACCACTCACTAAATGAGCGCCT
U5 8628537 8628586 50 AAGAAGGCCTGCCGCTTGGGAACTGGCTCTGGCCATAGGTGAGCATTGGT
U3 8628649 8628698 50 ATTCCACCCTTCTGAAAAGTCTTTAATTGGGGCCTGTTTCCAAGAAAACT
D5 8630239 8630288 50 TAACCATCTTGGGCTTTGGACACTGGCAGGCAAAAGAACTTTTGATTTGT
D3 8630315 8630364 50 AGTGGACTCCTGGGAGTTTAGACTTGGGTATGGTGAGGGTTCGGCTATCC
G3 8634341 8634390 50 TCATAGTGAAAAAAAAATAGTCTGCAAAGCCCTGTGATGCAATTAAGTAC
G5_U5 8623318 8628586 5269 GAACTCCTGTACAAATGACCTGTATACAACCACTCACTAAATGAGCGCCTGTAATGATTTCTGGAATAACTCCGAGCCTGCCTCTGTCTACCTCTGCTTTCTCTGAGATAGCTTTCTTGAGGTCTCAGTTAAATAGGAAACAGAACAACACAGATGGGGAAAAAAATGCCCGGACTGCTGTGGCACCTGACTTTAGAAGGTGTGCAGGGCCACCAGGTCTGGGTCGTCCAGTAGTTCACACACCACAGCAGTGCGGTGGCTGCTGCTCCTCCTGGACACAGTGACATCTATCTGCCTTCCATGACCATCCCTGAGTTCAAGAAGACGAGAGATGAGGCATCTGTGTAAGATTCTCTATGTCAGACTTAGCCAGGGTTGAGCGGAGTAAGGAGAAACAACATGGAGTCTTCAGCAAAGGGCTGAAGGCTAATGGAGACTAGCCTAAGACTAGAAAGAAATAACCAACAGTAGTCACCTGAAAACAGCTGTCAGGCCCAAGTATAAGGCAGAACCTGGTGGCCTCTAGGAAACACAAATACCCTCGAGGAGCAAAAGGCTGCCACAGAAGACCGTGGAATAGCATGGCAGAAACTAGGGGGAGTGGGGACTGCCCGAAACTCAAAGGGACAGGGACGAAATAAAATCTGCCTCGAAGGGAGTAAAACCCAAGGGTGACTTAGAGACAAATTGACAGGGTGGTGTGGTGCACATACTTCACACACCATTCAATCCACGCAGAAGGACCAGAGATGAAACCCCTCAGGAGAAGCAGGAGAAATGGAAAATAGGCAGGGCCCGTCATTCCCTGGCACCGCTGACCTGGAGGCCTAGAGACCCCAACAAGACAAGAATGAGGCACTAGAGCTTTCCTTGACTCACTCCTGACCGGGCATGGAAAGCTTAGGTGTGGAAGAGACTCAAACTACCCACCCTAGAAGGTACCAAGCCAGTGGCCAGAGTCCCTCTTGAGTCTTGGATTCAAAGAGCTGGGAGGTTTCCGGAGAAATGTTGGCTGGTTTGTTTGTTTTCAATGTGTCAACTCCTGTCCCTAAAGCACCAGAACTCTGGCTCATGAGGGCATCTAGAATAGGATTGGACCTGAGAACACCTTCAGCTGCCCCTCGCCCAGGGCAGCACCTCCCTCCTGCCTGCAGCTTAGAATCTCTAGAGCTGGTGATTCTCTTTCACAGTCGCGGTGACCAGCAACATATAGTCCTGATGGCTCCTTTCAAAGGCCCTTCTATTTTCTTTTTTTATAAAACCCCGGCCACCAAAGACAACCTAATGTCCCTTTTAAAGTTCAATGTTAAGCAGTCTAAAGAATTGTGCCCCTTTTAAAAGCCCATACTGAAGCCACCATTTTCTTTCATTGTTTGATGACAGAATTGTTTTCGTTGAAGATGTCTCTGCACTGATTTGGGATTTGGGGATGGGGATCCTGTGTCTGCCTGGTCAGCTTGGGGAAACAGGAAGTGGAGCAGGGCTAGGGTGGTATACTGGAACCTGAAGTTACTAGGGCTCATGTATCTGTGTTCTGCAACATGGATATATTATATTATATTATATTATATTATATTATATTATATATTATCCCCTCCCCTCCCCTTCTTTCAAGAAAGAAGACTCCGGGAGTTGTGAGGTCAGGACCCAGGATGGCTCCTCTCCTCTGTACTGGCTCCAAAGGAAAGGGGGCTTTGTTGTCTCAAATAGGGTGAGACCAGGGAAACTCCCCGTAGGAATTGTCACGGGCATACATTTATGGTTCCTGGGCCTTTTCCTTTGTTTGCTCTTGGTGGGAATGTTGCCTTGCGGAGGCTCAGGCACGAAGCTTTTCTGGGATAGTTGAAGCTTCTCCAGGACTGGCCAACTGGAGAGAGAAGCAGCCAGCAGTGCCCAGGCCACGCCTCTCCCAAGGAAGGGCAGGTTACTAACTGGGAATTCTTTACAGCTGGCCTGGAGGATGTTTTTGGCTGTAAACTGTCATGGCACCGCAACCCTCCTTGAAAAGGCTGAGGACAACTTTCTCGGGACAAAGATCAGAAATTAATCACAGAAAAGGGTAGGGACAGAATAGCTAAGCCTCAAAAGAGTCGCTTCTAGCCCGGAAACCAGGGATGTGGCACTGTGGAGTGGAGAGATTCCACTGCTCACGGTAGAGAAAGTAACACCATTTATTTGCCTCCTCCTCTTTGGAGAACAGTTCCAGAGCAATCTTCTACCAGCTTCCTGAACGATGGCGAAAATCCACTTTTGACCAACAACATTCCCAGGACCGGCTCCGCCTTCACGCCAGAGGTTAAACGCGTCAGTGACATATATTAGTGACCCAGCCTCCCTTGACTTTCATGTCCTCCTCCACCGAGATTTATTCCTAGTTCCAGGAAGTCTCAGGCCTTAGCGTCCCCGAGGGTAGAAAAGGTGAAATCTCCCTTGTATACTTCTGCAAACCCACAACACAAACTGGCTGGTGCTCATTGCAGGAGGGTCGGGGTTGGGCGCAAATGAGAGCCTTTTGGTGTCTGTAGAAAGAAGTGTCCCGCTCCTCTAGCCAGAGATTTCTTTATATCCTAGACCACAGAAAAGCAGCCGAACACCCCAGTATTCAGTGGGAAAGGGCAGAGGAGGGTAGTCTAGGGCATCCCACCCCAGAGGTTGGCTCCTGGAAAACCGTCTCTCCCAGAAGTAGGGGCAGGTAGAACCCACAACTCCGACCCCAAAGACTTCCCAGGGAGACTCTCAGAGAGACAACGAACTCAGAATTGAGTGCCCCCACCTGATTCAGGGGCCTCTTCCAAGGAGCTTCGGGATAGGATAGGAGAGTGGAAGACGGGGAAACGGAGGCTGGAACCCAGAGTCTCGTTAAAGAGCTGGGCGCGAGCTCTGGCTTCCTTCCCGCTTCCTGGGCTCCCGTTTTAGAGGAATGTTATTGTTTAAAGAGACCCCATTGAACTATTTCCTGCTCTTTGTCACCTTCCCCTCACTCTCCCGGCAGAGGTTCTCACCGAGAGGCAATAAACCAACTGCTGCCCACACCGCATGGCGAGGCGGGTAGGGAAACGCGCGCAGCATGCGTTCCAACAATCCCCGGCGCAAAGAGACCAGGGACTCCCGGGGCCACATTCGGTGCAGGCGCATCCACCGTCAAAGTCCAGCTTTTATGTGGGACGCGAGGACACCTCCTACTAGGGTCGCTATCTGTTCGTCTATTTCCCTCTCTGGACAGATCCGCATTGAGCTTCCCTCTCCACGCAGGTGAAGGTCGTGGGGGACCTGGATGCCGAGGTGGGAGTTAGTGGCAGTCCATGGGGCGAGGGGACGTGTCCCAAAGCTGCCACACCTGGGGAGGCTGAGGCTTTGGAGAGGTCAAGGAGACCCGGGAGACGCCGATCCGCCGAAGTCCCTCTCAGGTGCGCGCAGCGTGGACACTCCGCGGGGCAAAGTCGCAGGCGCCGGTGTGCGCTTGGACAGCGCGTGGGAGTGGAGAGTTTAGCAGTGGCTCTAGGAGCCAGGGTCCTGGGTGGCTCCAGCCCGGCTTCTCGCCCTCCCTCCCCCAGGGTCCGCCCCGCCGCTTTGATGGAGGTGCAAACATTTGGGGGAGGGCGGGGGTGTCGGGACTGCGCCCGACGCTTTCCTTACAGGAAGCGCGCGCTGGAGCCCATTGTTGGCCCCCAGCCTCCGGGCCCGCCCGGCCAGTCTGATATGGCCGCGCACCGCCAATGGGCAGCTGCTCGGTACTTCAAAGGGTGTCCCGCCCAATCCGCCGCACCCCCCTGCGAGGCCACCTCGGCTGTATTTATGGGGAGGGGACCCATTTTTCTCTTCCCCAGAGACTTACTCTTGTCGCGCCTTGCGGGAGTTCAAGTGGAGCCACGGCTCCCCAGGCCCTCTCCCCCATCCCCGCCCCCTTCCCCCCTCATCCCGATCTCGGTGCTCCTTTGGCGAATGTGCAGGGACCCAGGTGTAATCTTTGGGCTCCGCAGAGTGACCCTTTTTCTTGGAAAAGCGGTGGCGAGAGAAGTGAAGGTGGCTGTTGGGTAGGGAGTCAAGACTCCTGGAAGGTGGAGAGGGTGGCGGGAGGATGAGCTCGCCGGGCACAGAGAGCGCAGGGAAGAGCCTGCAGTACCGAGTGGACCACCTGCTCAGCGCCGTGGAGAGCGAGCTGCAGGCGGGCAGCGAGAAGGGAGACCCCACCGAACGCGAACTGCGAGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGCTAACTAACGAGATGATTGTGACCAAGAACGGCAGGTAGGTGCGTGCTGCAAGCCGGCAGTCTGGGGCCTGGCGAGTGCCCCTCGCCTCGGGGAGAGCATCTTTTCTTCCCAACCGGCCGACTTTGTTTCTTCCCGCTGAGTTAACAGCTCCCCAGAGGATTCCTAAACCTTGGTTTCTCGGGAACTTTCCCCCTGACGAAACCTGAGGAGGAAGGAGGCATTCGAACTCTAGGGTTCCTAGGTGTATACTGGAATGACCAGGTTTGCCTAGGGGGCCGAAACCCTTGGCTAGATTTGTCCCTCTGCTGTCCCGAGAACCCGTGGGCTTAGTGGGGGGAGATGATTTGGTTCCTGAACTGAGAAAGTTCAGGCAAAGATGCTTGCGAGACCGGTTGCCGGGGTCCTCCTCTTTGGCCGGCATCAAACACTTAGTGCAGGCTCCCCCTCTCCAACGGATGGGTCCTCCTGCCGTGCGCTGTCTTTTCCAGGAGGATGTTCCCGGTGCTGAAGGTAAATGTGTCAGGCCTGGACCCCAATGCCATGTACTCTTTCTTGCTGGACTTCGTGACGGCTGACAACCACCGCTGGAAATATGTGAACGGGGAGTGGGTACCTGGGGGCAAACCAGAGCCTCAGGCGCCCAGCTGCGTCTACATCCACCCAGACTCGCCCAATTTTGGGGCCCACTGGATGAAGGCGCCTGTGTCTTTCAGCAAAGTCAAACTCACCAACAAGCTCAATGGAGGGGGACAGGTAAGCCAGTGCAGAAGACAGCCTCTGGGCCCCAGGCCGAAGGGAAGAGCCTGGCTGGGAGCTTAGAAAGACACCACCAGCCTTTGTCAGCCCCACAGAAGCCACCGTGGCCTCCTTCCCCAATTAAATCTTTAGTACTGTTTATATTAAGCTGATGAGGGTTAAGCAGGGCCTGGCAAAGGGAACTGGGAGTTCTCCCTCACCTTGGAAGGGTTGAAGAAGGCCTGCCGCTTGGGAACTGGCTCTGGCCATAGGTGAGCATTGGT
U3_D5 8628649 8630288 1640 ATTCCACCCTTCTGAAAAGTCTTTAATTGGGGCCTGTTTCCAAGAAAACTCACTCCAAGTTGCCACCAAGCCAGGCATTTAAAGTTGTTTGTGGCCTTATGCCGGCAGGGTTTAATGGTGTATAGCGTATCTCCCATCCCAGTTCCAGACCATCTGTGGGAAGCAGATGTGTAAAATGGACCCCATGCCCTCCATACTGCTGGCAGTTTCCTGAAGACTTTCTGTGGCTGGATCCATGTTTCTTCCTGAGCAGTTCCTGTGGATGCTGCTGCGGTATTAAGGTAGGCCTGCACGCAGGTCTGCATTCTTGCTCTGTATCTCCTGCAGATCATGTTAAACTCCTTGCATAAGTATGAACCTCGGATTCACATCGTGAGAGTTGGGGGCCCGCAACGCATGATCACCAGCCACTGCTTTCCCGAGACCCAGTTCATAGCTGTGACTGCCTACCAGAATGAGGAGGTGAGGTTTCGGAAGGGAGTCCCCCTACCCTGCCTGTCCTGGGAACTGGGAGATAGAACCACAGTACTGAAGACAGGCAAGATTTAGTTCCCATTTACCACTTCTGTGGCTAAGAGGAAAAAACTTTAAAATACTCATTCGAGCATGTTGCTAATCTCAACAGAGCACACGTCTATGAAAGAAGCATAAACCACAACTTCACGGTTTTAAGATCTGGAGAGGAGAGGGGACAGCTCTTGATCTCAGCAACGGGAAAGTCCTGGCAAAGGCTGTCAAGGGTTCTTACTTCTAACCCTAAATCATAGAAAATGTGTCATCAGTCCCCTGGGGCTGCCGCTGTTGACCTTAACTCCACAGCAATTCCAGGCTATGTTGGGACTGTCATGATATCTAGCCTTGATAGCAATTATGTTAAATTGGAGTTTCACTTAGAAATTGCTTCTCTTTGTAATTTCTCATCCCTTTCAGACACAGATAGAAAAACAAAGACCAATTCATTCTAACTTGGCTGTTTTCTTTTGTTTTTTTTTTTTTTTTTTCTTTCTCAGTTTCCTTTTCTCTTCTTTGGTAGTGTCCCCCCACTCCCATTTGATGCTTGCTATGTAACCCAAGATGTTCTTCAGTTCACAGTCCCCTTACCTCAGCATCGTAGGATGCTTCTCCACCCCCCCCCATCAAATACTCCGGTAATTAGCATTGTATTGTTAGTAAATACACTTTCTCTTTTTTCAGATTACAGCCCTTAAAATTAAATACAACCCATTTGCTAAAGCCTTCCTTGATGCCAAAGAAAGGTGAGAGTTCTAACCTTGCTCCTGGGGCAAAGGACCGTGTGGAGAGAAGGCTGGTTGCTCACCTCCTCTGGGCAGGCCTCCTTTTGACCATCCTGAATACTTTTTGGCAGAAACGACCACAAAGATGTAATGGAGGAACCGGGGGACTGCCAGCAGCCGGGGTATTCCCAATGTACGGTTTGTTGCACCCCCATATTCCTCTGCCTTGTTTCAGGCCTAGACTTGCTAAAACCAGAACCCATAGGGAGGTTTGGGTTTCTGGGAATAGTGGGGGTGGCAGGATGGCTCTGCTTTGTGCCCTGGGCAAAGCGTGCCCTTTGCTTTGGAAGCTGCCTAACCATCTTGGGCTTTGGACACTGGCAGGCAAAAGAACTTTTGATTTGT
D3_G3 8630315 8634390 4076 AGTGGACTCCTGGGAGTTTAGACTTGGGTATGGTGAGGGTTCGGCTATCCCCCTCCCCCACCTTTGAGATGGCTTCCACTTGTTAGTGGCTGAAAACTGAATGGCTATCTTTGGATACACCTATGAGCGGCCTTCCTGCTCAGTTCCTGTGCCTCCTGATTTTGGTGGCTTTTATGGTGGCTAGTTCTTTAAAAATATCTCTTTAGGAGGTCTCCTAGGCAGTGATGGGTAGAGGTCTGTTCAGGCACATGGAGAGAAAAGGCCTGGTCTCGTCCCTTTTTCCAAGGAGGGATTTTCAAGTATTGAAGTTGTTGCTGAGGGGTAGAAGGGTCTTGCAGGCCATTTCAGTGGCTGCCAGACCAGCCTAGCACTCTGTCCCTAAACAATGATCAGGTATTTTAATACCATCTTCTAAACAAATTTAACAAATGAATTTAACGAACAGTTCCCCTATTGTGGCTCATCGGGTAGTCACCTGACCAGGAAGAAATGGACGGTGTATGGGGCCAGTGTGGACACATGTGTGCTCCTCAAGTATTGTCTCAAGTATTGTATCGGGTAGTCACCTGACCAGGAAGAAATGGGCGGTGTATGGGGCCAGTGTGGACACATGTGTGCTCCTCAAGTATTGCCTGAAGAACTGTTTCTTAAAGTAGAGAGCAAAATTAGCTTCAGTGGATAGATCTGTCTTGGAGGTGTAGTGGAAAGAATGTGGGAGAGTCAAAACAGAAGGGAGCAGATAGGAAATGGACCCATTAGCTATTCTTGAGGCAGGAGCATGACTACTGGCCTCAGTAGCTGGGGAGTGGGAAGCTGTCTGGAGCAGTCTGAGTCCTACCTGTGGCCCACTGGGTGCCTCTCCCTTTATTCTAGAGCCCATTAACATATCACTCCTGCTCACTTGGTAGAAAGCCACCGGTCTGCAGAACCGAAATTTCACAAAGGTTAGGAGAGGTCTTAGGTCTAATCCTGTAAGACAGGGCTATGGGCGGAGCTCAGGCCTCTTGAGCGCTGGCATCCCGGGTGCCACAGGGACACTTTGAAACTTGGCTGCACTGAGGGAGGGATACTGGTTATTCTTCAGTTCCAGAGTCCCTGGGTTAGAGCTAGGACAGGACTGAGTCTCAAGCAAGGGCCAGTTACCCAGCATGCTTTTCTCACAGCAGGGGGGTGGCTTGTTCCTGGTGCTGGCACCCTCTGCCCGCCTGCCAGCTCCCACCCTCAGTTTGGAGGCTCGCTCTCTCTCCCCTCCACACACGGCTGTGAGAGGTACCCAGCTCTAAGGAACCACCGGTCATCGCCCTACCCCAGCCCCTATGCTCATCGGAACAGCTCTCCAAGTAGGTCCTGTACATCCATTTACCAGCTGGGGGAGGGGTGCCGGGGTGGGGCGGATCCCTCCGGAGAGGCATTTCTATAGGGGTGCAAGATAGGGAACCCAGCTCCTTGTGGCTCCTGGAGAGACTTGGTCAAGATGCTGGTTGAACCAGGGGTCCCCAAGGAGGCTTTCATTTCAATATCCATGTGCCTCAGAACATGCCCCGGGCTTTTCAAAAGACAGCTGTCTGGGCCTTTCCAAGGAGCCAAAGCTTGAGCCCCAGAATGGAGCCGTGGTTTGATGAACATCAAGGTTCCATGAACCTCTCTAAAGTGACCCGATTTGACTAGCCAGGTACAAACGTTCCTGACCAGATCAAAGACTCTCCAGGTGTAGAACTCAAGCCCTTAGTGTGGGTGTCACCGGAGAACCAGGCACATTTTAGGTCCACCCTGGACCCAGAAGATGGACCTAGGCGCAGGACAGACCTCTGGCAGATTCTTTAACACTTGTCTGTTTAGACCAACCTTTGTCTGCTCCAAAGGGAGGGGAAAATGGCTAAAGAACACCATAAAACAATTAAGCAATTACAACTGATTCAAAATGAAAGGCCACAGTCTTGGAGACTCCTCCATCAGAGGCTGGGCTCTGAGGCGGGGGAGATCATATTTTTAATTTGGTGAGGACATGGTTGCCATAGCCTCTCCGAGCCTTGCGGAATCGCCATTATGGAAAAGGTGAGCTAACGTGGCAGATATTTTTTTATTTGCTTTTAAAATTAAGCATTCCAGGCTAGGGTGTCACTCAGTGGATCAGCACTTGCCTAGCGTTTGAGAGGCTCTGAGTCAGATCCCTAATGCTACAAAAATAATAAATAAATAGCATAAAAATAAAGATCAATACCACTGTCTGAAGGACATCTCCTTGTGGGATGATAAGAGACACCTTAGGTTAAGTGGGATATATTTTCTAATAATTGACACTGACAATAAATATTGCTGTGCTGATGAGCTAAAGCAGGCTAGGGGCTTGCTCTCCACATCTACCCAGGTAGCTCTTGTTTGCAGTGTTGGGTTCAGTTGGTATCATTTTATTCTGTTTGAAAGACCTGTCAGGTAACTTCCCCTCTCTGCATCCCTCAGCCTATGCGGACAATTCATCTGCTTGTCTGTCCATGCTGCAGTCCCATGATAACTGGTCTAGCCTCGGAGTGCCTGGCCACACCAGCATGCTGCCTGTGAGTCATAACGCCAGCCCACCTACTGGCTCTAGGTATGTATGTCTGTATGTAAAAAGCAGCTCAGGCCTCAGTCTGTGTGTGTCTCTCAGGCTGATGGGTTTGACATGGGTAGTTTAAAGAAACAGTGTAGACCCTTTAGTCACTGGAGGCAACACATTGGCTTTTTCTGTGGACAAAGAAATAGAAAGTCCTTAGTGCCTCCAGTGCTGAGAACCAGTGAAGGATGAAGACTGTGGACTGTCCCGCAGGCTATAGACGAGGCTGCTGGGGATACGTTCCCTTCAGGCTTGTTAACCCTCCAGGGTACTGAAACAACAGTGAAGAACTGCAAAGCTAGATGCCAATACTCCATCTCCAGTGAGGAAAGGGACCTGAAGATACTGATTCTGGGTCAGTGTGATGATGAACTGTCTGTTCTCAGAGGAGTGAGCCTGCCATCATACAACCTTGACCCCAAATGTCTAAACCAGTGGTCCTCAACCTTCCCAGTGCTATGACCCTTTAATACAGTTCCTCATGTTGTGGTGACTCCCAACCATAGAGTTATTTTGTTGCCACTTCATAACTTATTTTTCTGTTATGAAGCATAGTATAAATATCTGATATGCAGGATATCTGATAAAGGGGTTGCAATCCACAGATTGAGCAGTAGTGGTCTAGAAAATTAAACATTTAGCTAAGGCCCTAGCTGTTTTGGCTTTGGAGCTAGCTCTTCACTAATAAGAATTTAGTTGTCTCGTGTTTCTTTGTTTGGTTGGCTTTGTTTGGTTTTTGACATAGGTCTCAGGCTGGCTTTGAACTCACTACACAGTCCAGAGCTCAAGGTCTTGATTCTCCTGCCTCAGCCTCTTTAGTTGTTGGGGTTATTGGTAGGTATTACTAATTCCACTTTAACCTGCTCTTAAGGCAAACACACAGATGACAAACTTCTCAATGGCCCTGAGGACTTTGCTTAAGGACCCAAGTGATCGGCAGGCAGCCAAGTGCTGATCACTCGTTCCAATTTGGTGTCTTAGTCTGAAACAGTAATCTGCCTTGATTTACCCAGGCTTGCTCTAGAGTCCGAATGCCTTTGTAGATGCCAGAGGGTTTAGCACTCATTTCAGCATCCTCGTTTTATATCCTGAAAAACAGTCATGGGGCAAGGTCAAGACGCAGAACTGGTCTTCAGCCCAGCTCTGGGGTCCCTGGCGTATTGCCCCATGCTGACACGCTAATCCCCTGTCTACTGGACAAGCCTGTGACAGCAATGATGGCTTTCTAAAGAATATGCATTCTGTGAATGCGGGGATTGTGGGGTGGGGTTGGGAACACATTGCCCTGAGCCTTGGTGCTCAGTGCTAATCCTGTATGCCAATGAGCACAGAGCTACTTGTCAGAGTCCTCTTTAGGAGGACTCGGCACACTAAACAGTCACTGTGCTTATGGGCACCTTATTGGTTTTGTTTGATGCAGTCCATCCCTGTGGTCATAGTGAAAAAAAAATAGTCTGCAAAGCCCTGTGATGCAATTAAGTAC
U5_15 8613538 8628586 15049 GAGGTGGTTAAGTCTCATAGCCTTGCGGAGGCAGAACCCTCCTTAAAACATATCACATTTCATTGTGGATGCTATTGTCTTGGAGAGAGGACGCTAATGAATCTTTTTTTTTTTTTTTTTAAATTGTGGTATTGACAGGGCTGGAGAGATGGCTCCACAGTTAAGAGCATCCACTGGCTGCTCTTACAGAGGACCCAGGTTCAATTCCCCACACCCACATGGCAGCTCACAACTACTTACTACTCCAGTTCCAGGGGATCCAGTGCCCTCTTCTGGCTTCCATGGGCACTGCATGCATGAGATTCATAATGCAGGCCAAGCATTCATTTACATTAAATTGTTTTTTAACTACATTGAGGAAATCTGTGTACTGGCTGGTTTTGTGTGTCAACTTGACACAGGCTGGAGTTACCACAGAGAAAGGAGCCTCAGTTGAGGAAATGCTTCCATGAGATCCAACTGTAAGGCATTTTCTCAATTAGTGATCAAGGCGGGAGGACCCATTGTGGGTGGTACCATCTCTGGGCTGGTAGTCTTGGGTTCTATAAGAGAGCAGGCTGAGCAAGCCAGGGGAAGCAAGCCAGTAAGTAACATCCCTCCATGGCCTCTGCATCTGCTTCTGCTTCCTGACCTGCTTGAGTTCCAGTCCTGACTTCCTTTGGTGATGAAGAGTAATGTGGAAGTGTGAGCTGAATAAACCCTTTCCTCCCCAACTCACTTCTTGGTTATGGTGATGTTTTGTGTAGGAATAGAAACCTTGACTAAGGCAGTCTGTGACCCCAAAAAGTTAATGTCATAAAAATGAATAAAATGGGGGAAAGGCCTTTTATTTGCAGGAAAAAAAGTCTGTACTGATGTTAAGAGCATTTTGAGTTTGTGTACAGAAAACAGTCTGGATGTCACCTATGAGAGAATCTCTCCTCTCTTTCATTTAGGAGCAAGCTCTCCGAACACGAGAGGGGCACACTTGCTCCTGCCAGGAGGACCACAGATCCCAGACCTACAGCACCCCTGACCCTTGCACACGTGATCCAGACCCATCCTGTGGTGGTAGGTTCCTGTTTTCTCTCTGCCTGTGCTGGGCGGAGGCTGTAGCTCTCCATGAGATCAAAGGACTGATGAGAGCAGGAGATGGTGGAAGGCCAGGAGGTGGGGCAGAGCAGGATGCCAGCAAGTGTGAGATTTCTGAGGATTTCCGGGCTGGAGATTAGATGGGCCAGCAGCAGCAGTGAGAGATGAAGTATAGGAGATGTCAGGGTAATGGTAGGCACCAGAGAGGAAGGAATAGCTCTCTAACGGCTTTCAACTCCCTGGGTGAGTTGGCACTGATAGCAAAGTTAGCGCACAGTGCAACCCTCAGAGTGTATTGTTGAGAACAACGAAAGAGTTCACGTAAATACCTGTTTTTTTTTTTTCCGTTCATTCACTGTAGGAGGCCAAGACAGCAAGTCGATACTGGGTGAACAATGTGGATGAAGAATAAGTTTTTTTTTTGTCTTTTTTTTTTTTAAACAGTCATTAGATAAAGTCCAAACAGGATATGACAGCTTTCTTTTTCTTTGAGTCTGCATTATGATAAGCAGCGAGGTAGGTATCCGGTAGAGATGCTCGTGCCTGACACGAGATGCCTGGCACTTCGTTCTTTCATTATTATTTTTTAAGATTTATTTATTTATTATATGTAAGTACACTGTAGCTGTCTTCAGACTCGTCAGAAGAGGGCATCAGACCTCATTATGGATGGTTGTGAGTCACCATGTGGTTGCTGGGATTTGAACGCAGGACTTTAGGAAGAACACTCAGTGCTCTTAACCGCTGAGCCATCTCTCCAGCCCTTGTTCTTTCGTTGTAAAGGTGACACCATCATGCAGAGCCCGAACCGTGAATTTGGATGATGGAGACAGTCAGGCAGGCAGCACAGTGGGCAGCAGCGCTGAGCAGACTCAGACATGAGGCTCCAGCCCATGGCTAGTCACTTCTTCTGACTCATGGTCCTTCTGCCTGGTGCTCTCTTTGCAGCATCTTAAATAAAATAAGCTTGTTTTCTTTCTTAAAATTTGTTTAAAGATTCATTTAAAGATTCATTTATCTATGCATATGAGTGCTCTATTTGTATGTATGCCTGCATAACAGAAGAGGACATCAGAACTCCTTAGAGATGGTTGTGAGCTACCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAGAGCAGCCAGCGCTCTTAACTGCTGATCCATCCCTCCTGCCCTTAAAAATTATTTTTAAAACAGCTTTCAAATTATGGGTAAGGATAAGGAGGCATGAAAGTGAGCCAAGGGTGTTTGATGAAGTGAAATGAGCGTTATAAGTAGAAAAATATAAGGAGCGGAAGTAGATTCCAACAAGGGGTTGGATTAGGCATATCTGAAGATGGAATGTGTGAACCAGAGAGGCCAGAGATGTGATCAGATGTGAGCACCAGAGACAAAGTGCTGGCCTTAATAGCCCAGCGGAGCTGAAGCCCAGGGCCAGCAGTGATGGCAGAGAAGCTGTGGAAGGCACTGTGCTGAACAGAAAGGAATTTCCAGAAGGGGTGAAAAATATCAACCCTAATATTTAGGGAACCCAAAAAAATCACAAGCACTAGTAGAAATGACAGCATTGTCTGTGGATGTGGCTGAGTGGACGACTGCTTTACTTGATGCTCAAGGCCCTGAGTTCAATCCCCAGTATTGCAAGAATACTAATAAAGGGGGTGGGGGGAGGAGATGGCTCAGCACCCTGAACGGGAAAATCCCAGCTGTGATTCCAGTGTCAGGGGACAGATGCCCCCTTCTGGCTTCTGTGGGTACTGCATGCACATGGCACACAGCCATTCAAGCAGGCAAAACACCCATACATGGAAAAGATGAATAGTTAAAAATTTTTTTTCATCCTGGTGTTGTGGTCCAGCACTCAGGAGGCAGAGGCAGGTAGATCTCTGAGTTTGAGGCCAGCCTGGTTTATAGAGGGAGTTCCCTTGGTTGGACAGGGAGGGGGGAGAACAATAAAAAAAGAAAAAGAGGGGGAGAGAGAGAGGGGGGGGAGAGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAAGGAAGGAAGGAAGGAAGGAAGGAACGAAGGAAGGAAGGAACGAAGGAAGGAAGGAAGGAAGGGATTTTAGAAGAACAATGGAAATGCTTAAAGGAGCCAAAGAAAAAGGCAGGTCTCACCAAGGGATAACACCCAAAGTGCAGACAGCTCCCTAGTGGAAGTAAGGTCAGATTCGGAGAGAATCTTTTTAAGAGGTAGAAACCCTTAGGAGTCCTATAACAATTCAGCCAAGCCCATCACTTTCTTTCTACCTCTAAGAAAATAAGAAAATGTTAGGCACACGAAGCCTGCAGATGCAGAGGGAGGAGACAACAGAACTCAAACATACATTCTCTAGAGAGAGGGCCTAAGGAGCAGTTCCCAGCTCCCCCTGGGAGGGCAGTGCATCCTCAGTCACAAAGCCAGAGCTACACAGTTGTTCCTCCCAACCCAGGCACCACTCCTAAAAACACAGTGGCAAACCAAGAACTAAGCCTGTGTTTAAATATATGTAAGAAACCTGTGTGTTCAACAGTGAGCTAGCGTGACCTTTTCTCTTGAAGTTAGGGACAGCACCAGGCTCTACTTCATTTCCACTCCTGTGCAATACTGTAGGAGAAGCTTGACCCACACAAGGATGGGGAAAAAGGATAAACACTATCGTCGTTTATAAGTTTTAATTTTATCTTATGTGTACAGGTGTCTGTGCAGCACATACACACAGTGCCTGAGGAGTCCATAGGGGGATGAGGGATCCCCTAGGACTGTAACTACAAGTGTTTGTGAGCTGATGTGTCAGGGCTGAGAGTTTAGCCTGGGTCCCCTAGAAGAGCAGCCAGTGAGCTTAGCCTCTGAGCCATCTGTCTAGCCCCATTGTATTGTATAGAGTTTAAATGCTACAGGAAAACATAGCTGTGTTTAACGTTTGCAAACATTTCCTCTATGAATAAACAGAATTTTTTAAAAAAATAACTTTTAAATGTGTGTGTGTCCGTGTCCGTGTCCGTGTCCCTGTGATTGGCCAGACATGTCCAATCTCCTGGTGTTACAATTATAGGTGGTTGTGAACTGGGGACTGTGTGGTTGCTGGAAACCAAACTTGGGTGCTCTCAAGAGCAAAGGTATTCTGCACCACTGAGCCATCTCTCCAGCCCAGTAAATAGAACTTTAAAGACACTTGAATACCTTTTAACTTTAGGTGAGACTCTTGTCTCAGTCTCAGAAGGTCCCAAGGAGGAGAAGACAGACCCAGGTTCTGAACTGGCACTTGCAGTTTCCTCAGAATGAAACAGAAGCTATGTTCTGGCCCTTGCTTTCTTGCCCACACCTTCTGAGGCATGTCACCATTGGCCACCTGGGAGCCAGCTGGTATTTCAGGAATACAGTTGCCCCTCATCCTCTTCATTTTATAGCCCTTCAAGGAGACAGGCTGTGCTTAACTTTAAGACACAGTAAGAAAAGCTGACACCTTGGAAAAGTATGATGAAGATGAAGGAACAATTATATAACTATTTAAGGAGGAAAATGGACTGTCACGTCATACTGGAAACAGAATTATAATCTGTAGAAATATATGTTTCCGTGGATGAGTTGTCTGAGCTCTTGGGAAAATCAGAAAGCTAGTTACAGGGTTGAGCTAGGCATGAGGTCAGAAAATAAGAAGGTCATAGAATTTCTTCATTCCACACTGAAAAATACATTGAGTACAGTTAGCATGTCGTTGGTGCAGTAAGGATACTCATGAGGTGTGTGGGGGGGGGGCAGGAAGGAGGGAGAGAGAAGAGAGAAAAAGAGAGAAGGAGGGAGAGGAGAAAGAGGAGGGAGGGAGAGAAAGGATGGATAGGGGGAAAGGGAGAGAGAAGAGAGTGGGAGGGAAAGAAAGAGGTGGGAGGGAGAGGTGGGAGAGAAGAAAGGAGAGCAGAAGGGGGAGAGAGGAGAGGGAGAGTGAATAAGAATCTCAGATTCTAGGGTGGTTGAGATGGGAAAAATATTTTGAGTTCGTGACCAGCCTGGTCAACATGGTATTGAGGAAGGAGGGACAGAAGGAAGGACTGGGTGGGAGGAGGGAGGACCTGGAGGAAAGGAAGGCAGGCTGCCACTTTCCTCATCAGCAGGTGAGCAAGAAGAGAACTGGTCTGAGTAAACACAGCCTGTTAGCACTGTTCTAAACAGCAGAGCTCTGCCTAGGCAGCACAGACCCCAGCAGGTTGGAAACTAGCCAAACCAGTTTATACAAGTTTAAGGATCCCTTGGCTAAAATGCTTGTGACCAGAAGTGTTTAAGACTTGAATCTTCTAGGTTTGGGAATATTTGACTTTAATGGTTGAGCATCTCTCAATATCTTTCTCTTAAATACCTTGTAACCGAGGCTGGAAAGATGACTTAGTGCATTTTTTTTCCCTCTTGCAAAGGGCCCTGGGTTTGATTCTCAGCACTCACATGATGGCTCACAACCATCTCTAAGGCCAGTTCCAGGGGGTCTGATGCCCTCTTCTGACCTCCATGGGCACTACACATTCGATATGCTTACATTCACGCAAGCAGAATACGCAAACCCATACAATAAAGTAAAATAAACTAGAAAATAATCACAACAACAAGAAAAACCCACTTTGCTGTGGCAGCGTAGAGATAGCGGCTGGGTAGGAGAGCAAAGGGAATCCTGTCCAGCCTCAGTGGACTCTCCTGATCCTCCGGGTCCCCAGCTGGCATGGAGTTGGAGTCCCACATTTACCAGTGGCCTGGAGGGGTGACCATGGCCCTCTTTGGACTTTCATCCATTTGTGGTAAGATCATCATCTCAGGGACAGACTTCTGAGAGATGTTCCATAGTGGATTAAAACCAAAGCAGGAGGGGCTGGGGAGATGGCTCAGAGGTTAAGAACACAAGTTGCTCTTTCAGAGGACTGGGTTCAAGTCCCAGATCACATGTGGCAGATCATAGCTGTCTATAGCTCAAGTTTCAGGGGATCCAAAGCTCTCCTCTGGGGTGTGTGAATTTGATTCCTGGAGTCCACATGGCAGAAGGAGATCTGACCTCCATAATTTGCACCCTCCGCTTCTATGGTGAGGGCTGTGCCCACACACATAAATACACACTTACACACATATGTGCACACAATGTTATGTCTCACTACATAGCTCTGGCTGGCTTGGACTGGCTGTGTAGACCAGGCTTAGACTCAGGCTCATAGAGCTCTGCCTCCTCTCTGCTACCACGTGCACTGCCATGATTGAGAAACAAAACAAACAAATGATTTATTTTCTAAAATAAAGTCCTGGGGGACTGGAGAGGAGTTCAGTTTCCAGTGTCCAAGTTAGCTCAAAACTGCCTGTAAGCTGAGGCAGATAAAGCTTGCATGACCAATGGGCCTCTTTCCATTGATGGCCAACTAGGCCATCTTCTGATACATATGCGCGTGTGCGTGCACACACACAGACACACAAACACACAGAGACACACAGACACATACCCTACTACACACTACACACATGCATACACACACACATACACACACATTCCTTACCATATACCACACACATACACAGACACACACACAAACACACACCCTACTACACATTACACACATGCATACACGCACACATTCCATTACATACCGCATACACACACATGTATACACACAGTCCAATATACACTCCACACCACATATATACTATACCTACATACACATACACACACACTACACTATATCATACTATACCATACATACACACACACAGCACACACACACACACACACACACACACACACACACACACCACACCATACACTCACACACATACATACAGACCACATACACACACGCACACACACACACACACACACACACACACACACACACAAACACATGCTGAAGGGTTCCAGGCCTGAGCAGGGTAAACAAGGCACCAGCAAGCCTTGTCTTTCCCCTAAACGTTAGCTCACACTCCTGAAGCTAACCACCAAGGTCTATTCCCTTATTTGGCCACTTTCTTATACTGCCTCATTCCTGAAGCTGGTCACCTCACCAAAGCCCAGCAATCAAAAGCGTCCACTTAGCTACCTTAATTAAAATCTGCGGTCAGAGCCTACCAACCCACCCCAGCTCATTCTAACCCAGACCTCCCATTTTTTCCTCTTAAAATTAACTCCTACCAACCTTGTCTCTTTGCCCTGCTTAAGGATTACTCCATGTTGGAAATTGGTACTTGGGTGTGGTATGTGCCTAATCTGGGGCCCCTCCCACACACTGTTCAGGTACCATCACACATAATCAAAAATAAATTATAAAAGTAAAAAATCATGGTTAAGGGAGAAGATGGATTTTCCTGCTGATGTCTTTTGAACTATCCCCACTTGGTTTGTAAACTAGACACCTTTAGAGTCATCTAAGAGGAGGGAACCTCAGTTGAGAAAATGCTTCCATAAAATCAGCCTGTAGGCAAACCTTTAGGCCAAGTTCTTGATTAGTAATTGATGTGGGAGGGTGATGCTACCCCTGAGCTGGTGCCTGGCTGAGAAAGTCATACAGGAGCAAACCAGTAAGCCATACTCCTCCAAGGGCTCTGCTTCCTGGTCCTGCATTCAGACTCCTGCCCTGACCACCCTGGAGGTACAAGTTACAAGTGAAATAAACCATTTCTCCCCAGACTGGGTTTTGTCCTTCTGTTTTATCCCAGCGATAGAAACTCTAACTACTATAGAAATTGGTACAGAGTGGGGGTTTGCTGTAGGGAGATGATGGTGGCATTTGAATTTGGGGCTGGAAAAGCCATGGAACACTCAGAGGGCTGCTCTGTGGGAGCCTGGAACAGTAGTGCATATGACGACAGTCCTGGCTTGTCACGTTTCAGAGGGAGGCAAAAACTGATCTGGGCATTTATTCGATGTTTTGAATGAAGATTCTGTGGTTCCGGTCGGTGGGGCTGAAGAATCAGCTGTGATTAAGAAGAGACCAACATCGCTGAGGTGGAATCTTCTGATGCCCTGGGATTGTTTCCTTGGGGGCAGCATAGACTGGGTCACTGGTAGCTGGGTAGTGGTGGCACAGGCTTTTAATCTCAGCACTGGAGAGGCAGAGGCAGGTGGATTTTTGTGAGTTTGAGGCCAGCCTGGTCTACAGAGCGAGTTCCAGGACAGCCAGGGTTATACAGCGAAACCCTATCTTGAAAAACCAAAAATAAATAAATACATACATAAATAAGTGGAGTCGATGGACATGATGGGAAAGATCTGTAATCATAGAATTCAGGAAGCAGTGGTAGTTCAAGGCCAGCCTGGTTTGTATGGTGAGTTTCAGGCTAGCCTGGGCTACATAGTGGGCCCTTGCCTTTAAAAAAAGTAGCCAAGTCTATCCCATTTACCTTCCTCAGGTCAAATTCCTTTTGTAACTATTTGTGCGAACGTGGTGAGCTAGCAAGAGGAAAGCAAAGAAACCCCACTTTTACTTTCTACATACTTAAGGTGTTTCAGTAGAGATAAAATAGACTTAAATGAAGATAAACCCGAAGTCTTCTCATTTTCCACTCTTTTCTGCGAGCTTCGGGTGCCTTATCCACAGAAAGACTGCTATCCCCTACGGGGTTGTTCCACTCAGTGACTTGTGCCTACTTCTTCCCTGCATACAATATAGGCTTTCTGGTATTCGGAGGTGGGACTGGTGTCTGGAAGCCGGATTGTGGGTCGTGGCTTGCGCTAATGCAGACTTCCAAGTGGGCTTTCCTCTCTGGGCTCTTCAGCTTGGTGGAGTCTGGTGTTGGCATTCTCTCATTCTGAATCGGTTCTCAGGAGTGTGTTAAGAGCCCCCTCCCCATTTTTTATCAGGCTTGCCCTTGATATGTGCGGAGATTTATCTTAACCTTCGAGCTTCCTGCGGCAAAGCTCCCCCGGGGCCATTCGCTAGCTCACCTCCTCTTCGCCTCTTCCTGTTGCTTAGCTGTCAGCAGGTGGGCGCAGCCTCCTTCCTCACACCCACTCCTCCTGGAAGCGGCCAGCGGGTTTCTGCCCCACCTACCCCGCTGGTCGCCGACCTGGGATACTGTCACAAGTCCGTCATTCCCCTGCTCCTGTTACTGTCTTTCCCTTGAATGCTCCCTGGAAATTCCTATTAAAAGCCTCTAAATCAGTGTTCTTCCAGAATTTTTATGGGACTTCAAATTCTTTCTACCAGGACTCTGGAGATCACCAAATCTCCCCCTATCTAAGAAGCCTGATAGGCATCTGCCCTCTCAAGAGTCCTCCTTTGGACTCATCCCTCTGCCTTCTTGTTCTTTATCAGCTCCGAAAAGTCTCCCAAAAATGCCTGCAGCTCTGACCTCTGACCTCCCCTCCGTGGTTCTTTTGTAGTAGCTGCTGAGTCTTCTCTGTCATTTAAATTAGGCTCTGTCTCAGTTTGCCATTCTCCTCCTGGGGTCCTTGATTTCTGCTTTCACAAGCCCCCTGGGCCGTTAGGATTCCAGTGGTCGGTACACCTGGGGAAGCTATATACACTTGACCCAGGTTCTCCGGGGAGCTTGGCATACTCTCCTGTTATCTCCGCTGTCCTAGTGAATGTCATGTACTAGCTGGAGCCAAGAGCCTGCTGTGACGTGTGGTTTGGATATAATGGAGGGGGAAGCGGAGGGGAGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAAAACGAAGAACTCCTGTACAAATGACCTGTATACAACCACTCACTAAATGAGCGCCTGTAATGATTTCTGGAATAACTCCGAGCCTGCCTCTGTCTACCTCTGCTTTCTCTGAGATAGCTTTCTTGAGGTCTCAGTTAAATAGGAAACAGAACAACACAGATGGGGAAAAAAATGCCCGGACTGCTGTGGCACCTGACTTTAGAAGGTGTGCAGGGCCACCAGGTCTGGGTCGTCCAGTAGTTCACACACCACAGCAGTGCGGTGGCTGCTGCTCCTCCTGGACACAGTGACATCTATCTGCCTTCCATGACCATCCCTGAGTTCAAGAAGACGAGAGATGAGGCATCTGTGTAAGATTCTCTATGTCAGACTTAGCCAGGGTTGAGCGGAGTAAGGAGAAACAACATGGAGTCTTCAGCAAAGGGCTGAAGGCTAATGGAGACTAGCCTAAGACTAGAAAGAAATAACCAACAGTAGTCACCTGAAAACAGCTGTCAGGCCCAAGTATAAGGCAGAACCTGGTGGCCTCTAGGAAACACAAATACCCTCGAGGAGCAAAAGGCTGCCACAGAAGACCGTGGAATAGCATGGCAGAAACTAGGGGGAGTGGGGACTGCCCGAAACTCAAAGGGACAGGGACGAAATAAAATCTGCCTCGAAGGGAGTAAAACCCAAGGGTGACTTAGAGACAAATTGACAGGGTGGTGTGGTGCACATACTTCACACACCATTCAATCCACGCAGAAGGACCAGAGATGAAACCCCTCAGGAGAAGCAGGAGAAATGGAAAATAGGCAGGGCCCGTCATTCCCTGGCACCGCTGACCTGGAGGCCTAGAGACCCCAACAAGACAAGAATGAGGCACTAGAGCTTTCCTTGACTCACTCCTGACCGGGCATGGAAAGCTTAGGTGTGGAAGAGACTCAAACTACCCACCCTAGAAGGTACCAAGCCAGTGGCCAGAGTCCCTCTTGAGTCTTGGATTCAAAGAGCTGGGAGGTTTCCGGAGAAATGTTGGCTGGTTTGTTTGTTTTCAATGTGTCAACTCCTGTCCCTAAAGCACCAGAACTCTGGCTCATGAGGGCATCTAGAATAGGATTGGACCTGAGAACACCTTCAGCTGCCCCTCGCCCAGGGCAGCACCTCCCTCCTGCCTGCAGCTTAGAATCTCTAGAGCTGGTGATTCTCTTTCACAGTCGCGGTGACCAGCAACATATAGTCCTGATGGCTCCTTTCAAAGGCCCTTCTATTTTCTTTTTTTATAAAACCCCGGCCACCAAAGACAACCTAATGTCCCTTTTAAAGTTCAATGTTAAGCAGTCTAAAGAATTGTGCCCCTTTTAAAAGCCCATACTGAAGCCACCATTTTCTTTCATTGTTTGATGACAGAATTGTTTTCGTTGAAGATGTCTCTGCACTGATTTGGGATTTGGGGATGGGGATCCTGTGTCTGCCTGGTCAGCTTGGGGAAACAGGAAGTGGAGCAGGGCTAGGGTGGTATACTGGAACCTGAAGTTACTAGGGCTCATGTATCTGTGTTCTGCAACATGGATATATTATATTATATTATATTATATTATATTATATTATATATTATCCCCTCCCCTCCCCTTCTTTCAAGAAAGAAGACTCCGGGAGTTGTGAGGTCAGGACCCAGGATGGCTCCTCTCCTCTGTACTGGCTCCAAAGGAAAGGGGGCTTTGTTGTCTCAAATAGGGTGAGACCAGGGAAACTCCCCGTAGGAATTGTCACGGGCATACATTTATGGTTCCTGGGCCTTTTCCTTTGTTTGCTCTTGGTGGGAATGTTGCCTTGCGGAGGCTCAGGCACGAAGCTTTTCTGGGATAGTTGAAGCTTCTCCAGGACTGGCCAACTGGAGAGAGAAGCAGCCAGCAGTGCCCAGGCCACGCCTCTCCCAAGGAAGGGCAGGTTACTAACTGGGAATTCTTTACAGCTGGCCTGGAGGATGTTTTTGGCTGTAAACTGTCATGGCACCGCAACCCTCCTTGAAAAGGCTGAGGACAACTTTCTCGGGACAAAGATCAGAAATTAATCACAGAAAAGGGTAGGGACAGAATAGCTAAGCCTCAAAAGAGTCGCTTCTAGCCCGGAAACCAGGGATGTGGCACTGTGGAGTGGAGAGATTCCACTGCTCACGGTAGAGAAAGTAACACCATTTATTTGCCTCCTCCTCTTTGGAGAACAGTTCCAGAGCAATCTTCTACCAGCTTCCTGAACGATGGCGAAAATCCACTTTTGACCAACAACATTCCCAGGACCGGCTCCGCCTTCACGCCAGAGGTTAAACGCGTCAGTGACATATATTAGTGACCCAGCCTCCCTTGACTTTCATGTCCTCCTCCACCGAGATTTATTCCTAGTTCCAGGAAGTCTCAGGCCTTAGCGTCCCCGAGGGTAGAAAAGGTGAAATCTCCCTTGTATACTTCTGCAAACCCACAACACAAACTGGCTGGTGCTCATTGCAGGAGGGTCGGGGTTGGGCGCAAATGAGAGCCTTTTGGTGTCTGTAGAAAGAAGTGTCCCGCTCCTCTAGCCAGAGATTTCTTTATATCCTAGACCACAGAAAAGCAGCCGAACACCCCAGTATTCAGTGGGAAAGGGCAGAGGAGGGTAGTCTAGGGCATCCCACCCCAGAGGTTGGCTCCTGGAAAACCGTCTCTCCCAGAAGTAGGGGCAGGTAGAACCCACAACTCCGACCCCAAAGACTTCCCAGGGAGACTCTCAGAGAGACAACGAACTCAGAATTGAGTGCCCCCACCTGATTCAGGGGCCTCTTCCAAGGAGCTTCGGGATAGGATAGGAGAGTGGAAGACGGGGAAACGGAGGCTGGAACCCAGAGTCTCGTTAAAGAGCTGGGCGCGAGCTCTGGCTTCCTTCCCGCTTCCTGGGCTCCCGTTTTAGAGGAATGTTATTGTTTAAAGAGACCCCATTGAACTATTTCCTGCTCTTTGTCACCTTCCCCTCACTCTCCCGGCAGAGGTTCTCACCGAGAGGCAATAAACCAACTGCTGCCCACACCGCATGGCGAGGCGGGTAGGGAAACGCGCGCAGCATGCGTTCCAACAATCCCCGGCGCAAAGAGACCAGGGACTCCCGGGGCCACATTCGGTGCAGGCGCATCCACCGTCAAAGTCCAGCTTTTATGTGGGACGCGAGGACACCTCCTACTAGGGTCGCTATCTGTTCGTCTATTTCCCTCTCTGGACAGATCCGCATTGAGCTTCCCTCTCCACGCAGGTGAAGGTCGTGGGGGACCTGGATGCCGAGGTGGGAGTTAGTGGCAGTCCATGGGGCGAGGGGACGTGTCCCAAAGCTGCCACACCTGGGGAGGCTGAGGCTTTGGAGAGGTCAAGGAGACCCGGGAGACGCCGATCCGCCGAAGTCCCTCTCAGGTGCGCGCAGCGTGGACACTCCGCGGGGCAAAGTCGCAGGCGCCGGTGTGCGCTTGGACAGCGCGTGGGAGTGGAGAGTTTAGCAGTGGCTCTAGGAGCCAGGGTCCTGGGTGGCTCCAGCCCGGCTTCTCGCCCTCCCTCCCCCAGGGTCCGCCCCGCCGCTTTGATGGAGGTGCAAACATTTGGGGGAGGGCGGGGGTGTCGGGACTGCGCCCGACGCTTTCCTTACAGGAAGCGCGCGCTGGAGCCCATTGTTGGCCCCCAGCCTCCGGGCCCGCCCGGCCAGTCTGATATGGCCGCGCACCGCCAATGGGCAGCTGCTCGGTACTTCAAAGGGTGTCCCGCCCAATCCGCCGCACCCCCCTGCGAGGCCACCTCGGCTGTATTTATGGGGAGGGGACCCATTTTTCTCTTCCCCAGAGACTTACTCTTGTCGCGCCTTGCGGGAGTTCAAGTGGAGCCACGGCTCCCCAGGCCCTCTCCCCCATCCCCGCCCCCTTCCCCCCTCATCCCGATCTCGGTGCTCCTTTGGCGAATGTGCAGGGACCCAGGTGTAATCTTTGGGCTCCGCAGAGTGACCCTTTTTCTTGGAAAAGCGGTGGCGAGAGAAGTGAAGGTGGCTGTTGGGTAGGGAGTCAAGACTCCTGGAAGGTGGAGAGGGTGGCGGGAGGATGAGCTCGCCGGGCACAGAGAGCGCAGGGAAGAGCCTGCAGTACCGAGTGGACCACCTGCTCAGCGCCGTGGAGAGCGAGCTGCAGGCGGGCAGCGAGAAGGGAGACCCCACCGAACGCGAACTGCGAGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGCTAACTAACGAGATGATTGTGACCAAGAACGGCAGGTAGGTGCGTGCTGCAAGCCGGCAGTCTGGGGCCTGGCGAGTGCCCCTCGCCTCGGGGAGAGCATCTTTTCTTCCCAACCGGCCGACTTTGTTTCTTCCCGCTGAGTTAACAGCTCCCCAGAGGATTCCTAAACCTTGGTTTCTCGGGAACTTTCCCCCTGACGAAACCTGAGGAGGAAGGAGGCATTCGAACTCTAGGGTTCCTAGGTGTATACTGGAATGACCAGGTTTGCCTAGGGGGCCGAAACCCTTGGCTAGATTTGTCCCTCTGCTGTCCCGAGAACCCGTGGGCTTAGTGGGGGGAGATGATTTGGTTCCTGAACTGAGAAAGTTCAGGCAAAGATGCTTGCGAGACCGGTTGCCGGGGTCCTCCTCTTTGGCCGGCATCAAACACTTAGTGCAGGCTCCCCCTCTCCAACGGATGGGTCCTCCTGCCGTGCGCTGTCTTTTCCAGGAGGATGTTCCCGGTGCTGAAGGTAAATGTGTCAGGCCTGGACCCCAATGCCATGTACTCTTTCTTGCTGGACTTCGTGACGGCTGACAACCACCGCTGGAAATATGTGAACGGGGAGTGGGTACCTGGGGGCAAACCAGAGCCTCAGGCGCCCAGCTGCGTCTACATCCACCCAGACTCGCCCAATTTTGGGGCCCACTGGATGAAGGCGCCTGTGTCTTTCAGCAAAGTCAAACTCACCAACAAGCTCAATGGAGGGGGACAGGTAAGCCAGTGCAGAAGACAGCCTCTGGGCCCCAGGCCGAAGGGAAGAGCCTGGCTGGGAGCTTAGAAAGACACCACCAGCCTTTGTCAGCCCCACAGAAGCCACCGTGGCCTCCTTCCCCAATTAAATCTTTAGTACTGTTTATATTAAGCTGATGAGGGTTAAGCAGGGCCTGGCAAAGGGAACTGGGAGTTCTCCCTCACCTTGGAAGGGTTGAAGAAGGCCTGCCGCTTGGGAACTGGCTCTGGCCATAGGTGAGCATTGGT
D3_15 8630315 8645365 15051 AGTGGACTCCTGGGAGTTTAGACTTGGGTATGGTGAGGGTTCGGCTATCCCCCTCCCCCACCTTTGAGATGGCTTCCACTTGTTAGTGGCTGAAAACTGAATGGCTATCTTTGGATACACCTATGAGCGGCCTTCCTGCTCAGTTCCTGTGCCTCCTGATTTTGGTGGCTTTTATGGTGGCTAGTTCTTTAAAAATATCTCTTTAGGAGGTCTCCTAGGCAGTGATGGGTAGAGGTCTGTTCAGGCACATGGAGAGAAAAGGCCTGGTCTCGTCCCTTTTTCCAAGGAGGGATTTTCAAGTATTGAAGTTGTTGCTGAGGGGTAGAAGGGTCTTGCAGGCCATTTCAGTGGCTGCCAGACCAGCCTAGCACTCTGTCCCTAAACAATGATCAGGTATTTTAATACCATCTTCTAAACAAATTTAACAAATGAATTTAACGAACAGTTCCCCTATTGTGGCTCATCGGGTAGTCACCTGACCAGGAAGAAATGGACGGTGTATGGGGCCAGTGTGGACACATGTGTGCTCCTCAAGTATTGTCTCAAGTATTGTATCGGGTAGTCACCTGACCAGGAAGAAATGGGCGGTGTATGGGGCCAGTGTGGACACATGTGTGCTCCTCAAGTATTGCCTGAAGAACTGTTTCTTAAAGTAGAGAGCAAAATTAGCTTCAGTGGATAGATCTGTCTTGGAGGTGTAGTGGAAAGAATGTGGGAGAGTCAAAACAGAAGGGAGCAGATAGGAAATGGACCCATTAGCTATTCTTGAGGCAGGAGCATGACTACTGGCCTCAGTAGCTGGGGAGTGGGAAGCTGTCTGGAGCAGTCTGAGTCCTACCTGTGGCCCACTGGGTGCCTCTCCCTTTATTCTAGAGCCCATTAACATATCACTCCTGCTCACTTGGTAGAAAGCCACCGGTCTGCAGAACCGAAATTTCACAAAGGTTAGGAGAGGTCTTAGGTCTAATCCTGTAAGACAGGGCTATGGGCGGAGCTCAGGCCTCTTGAGCGCTGGCATCCCGGGTGCCACAGGGACACTTTGAAACTTGGCTGCACTGAGGGAGGGATACTGGTTATTCTTCAGTTCCAGAGTCCCTGGGTTAGAGCTAGGACAGGACTGAGTCTCAAGCAAGGGCCAGTTACCCAGCATGCTTTTCTCACAGCAGGGGGGTGGCTTGTTCCTGGTGCTGGCACCCTCTGCCCGCCTGCCAGCTCCCACCCTCAGTTTGGAGGCTCGCTCTCTCTCCCCTCCACACACGGCTGTGAGAGGTACCCAGCTCTAAGGAACCACCGGTCATCGCCCTACCCCAGCCCCTATGCTCATCGGAACAGCTCTCCAAGTAGGTCCTGTACATCCATTTACCAGCTGGGGGAGGGGTGCCGGGGTGGGGCGGATCCCTCCGGAGAGGCATTTCTATAGGGGTGCAAGATAGGGAACCCAGCTCCTTGTGGCTCCTGGAGAGACTTGGTCAAGATGCTGGTTGAACCAGGGGTCCCCAAGGAGGCTTTCATTTCAATATCCATGTGCCTCAGAACATGCCCCGGGCTTTTCAAAAGACAGCTGTCTGGGCCTTTCCAAGGAGCCAAAGCTTGAGCCCCAGAATGGAGCCGTGGTTTGATGAACATCAAGGTTCCATGAACCTCTCTAAAGTGACCCGATTTGACTAGCCAGGTACAAACGTTCCTGACCAGATCAAAGACTCTCCAGGTGTAGAACTCAAGCCCTTAGTGTGGGTGTCACCGGAGAACCAGGCACATTTTAGGTCCACCCTGGACCCAGAAGATGGACCTAGGCGCAGGACAGACCTCTGGCAGATTCTTTAACACTTGTCTGTTTAGACCAACCTTTGTCTGCTCCAAAGGGAGGGGAAAATGGCTAAAGAACACCATAAAACAATTAAGCAATTACAACTGATTCAAAATGAAAGGCCACAGTCTTGGAGACTCCTCCATCAGAGGCTGGGCTCTGAGGCGGGGGAGATCATATTTTTAATTTGGTGAGGACATGGTTGCCATAGCCTCTCCGAGCCTTGCGGAATCGCCATTATGGAAAAGGTGAGCTAACGTGGCAGATATTTTTTTATTTGCTTTTAAAATTAAGCATTCCAGGCTAGGGTGTCACTCAGTGGATCAGCACTTGCCTAGCGTTTGAGAGGCTCTGAGTCAGATCCCTAATGCTACAAAAATAATAAATAAATAGCATAAAAATAAAGATCAATACCACTGTCTGAAGGACATCTCCTTGTGGGATGATAAGAGACACCTTAGGTTAAGTGGGATATATTTTCTAATAATTGACACTGACAATAAATATTGCTGTGCTGATGAGCTAAAGCAGGCTAGGGGCTTGCTCTCCACATCTACCCAGGTAGCTCTTGTTTGCAGTGTTGGGTTCAGTTGGTATCATTTTATTCTGTTTGAAAGACCTGTCAGGTAACTTCCCCTCTCTGCATCCCTCAGCCTATGCGGACAATTCATCTGCTTGTCTGTCCATGCTGCAGTCCCATGATAACTGGTCTAGCCTCGGAGTGCCTGGCCACACCAGCATGCTGCCTGTGAGTCATAACGCCAGCCCACCTACTGGCTCTAGGTATGTATGTCTGTATGTAAAAAGCAGCTCAGGCCTCAGTCTGTGTGTGTCTCTCAGGCTGATGGGTTTGACATGGGTAGTTTAAAGAAACAGTGTAGACCCTTTAGTCACTGGAGGCAACACATTGGCTTTTTCTGTGGACAAAGAAATAGAAAGTCCTTAGTGCCTCCAGTGCTGAGAACCAGTGAAGGATGAAGACTGTGGACTGTCCCGCAGGCTATAGACGAGGCTGCTGGGGATACGTTCCCTTCAGGCTTGTTAACCCTCCAGGGTACTGAAACAACAGTGAAGAACTGCAAAGCTAGATGCCAATACTCCATCTCCAGTGAGGAAAGGGACCTGAAGATACTGATTCTGGGTCAGTGTGATGATGAACTGTCTGTTCTCAGAGGAGTGAGCCTGCCATCATACAACCTTGACCCCAAATGTCTAAACCAGTGGTCCTCAACCTTCCCAGTGCTATGACCCTTTAATACAGTTCCTCATGTTGTGGTGACTCCCAACCATAGAGTTATTTTGTTGCCACTTCATAACTTATTTTTCTGTTATGAAGCATAGTATAAATATCTGATATGCAGGATATCTGATAAAGGGGTTGCAATCCACAGATTGAGCAGTAGTGGTCTAGAAAATTAAACATTTAGCTAAGGCCCTAGCTGTTTTGGCTTTGGAGCTAGCTCTTCACTAATAAGAATTTAGTTGTCTCGTGTTTCTTTGTTTGGTTGGCTTTGTTTGGTTTTTGACATAGGTCTCAGGCTGGCTTTGAACTCACTACACAGTCCAGAGCTCAAGGTCTTGATTCTCCTGCCTCAGCCTCTTTAGTTGTTGGGGTTATTGGTAGGTATTACTAATTCCACTTTAACCTGCTCTTAAGGCAAACACACAGATGACAAACTTCTCAATGGCCCTGAGGACTTTGCTTAAGGACCCAAGTGATCGGCAGGCAGCCAAGTGCTGATCACTCGTTCCAATTTGGTGTCTTAGTCTGAAACAGTAATCTGCCTTGATTTACCCAGGCTTGCTCTAGAGTCCGAATGCCTTTGTAGATGCCAGAGGGTTTAGCACTCATTTCAGCATCCTCGTTTTATATCCTGAAAAACAGTCATGGGGCAAGGTCAAGACGCAGAACTGGTCTTCAGCCCAGCTCTGGGGTCCCTGGCGTATTGCCCCATGCTGACACGCTAATCCCCTGTCTACTGGACAAGCCTGTGACAGCAATGATGGCTTTCTAAAGAATATGCATTCTGTGAATGCGGGGATTGTGGGGTGGGGTTGGGAACACATTGCCCTGAGCCTTGGTGCTCAGTGCTAATCCTGTATGCCAATGAGCACAGAGCTACTTGTCAGAGTCCTCTTTAGGAGGACTCGGCACACTAAACAGTCACTGTGCTTATGGGCACCTTATTGGTTTTGTTTGATGCAGTCCATCCCTGTGGTCATAGTGAAAAAAAAATAGTCTGCAAAGCCCTGTGATGCAATTAAGTACTGATTCCTCCCCCACATCTCATCATAGCTCATTTGCAGGCCCCTGATGTCTGTTTTCTCTATTTCAGCCAGTATCCCAGTCTCTGGTCTGTGAGCAATGGTACCATCACCCCAGGCTCCCAGACAGCTGGGGTGTCCAACGGGCTGGGAGCTCAGTTCTTTCGAGGCTCCCCTGCACATTACACACCACTGACGCACACGGTCTCAGCTGCCACGTCCTCGTCTTCTGGTTCTCCGATGTATGAAGGGGCTGCTACAGTCACAGACATTTCTGACAGCCAGTATGACACGGCCCAAAGCCTCCTCATAGCCTCGTGGACACCTGTGTCACCCCCATCTATGTGAATTGAACTTTCCTCCATGTGCTGAGACTTGTAACAACCGGTGTCAACTGGATCTTCTAGGCTCAAAGTGGCAGGCTCTTGGGACAAGGGAAAAATAAATAAATAAAAGCTAGATACTAACAACTCCATTTTCAAATAAGAGCAATAATACATGTCCTATAATCATGTTCTACAGCCTCTTGTTTGATACCTACAGTAGTGATATGTGTCCTACATTATGAAGCCAAGGACAGAGAGACGGCTGTGGTCCAGTTTTTTGTGACTGGCAGTTAATCAGAGTCCTTTGCTAGGTAGGGTCCTATATCTTGTGTTTCTCTACAACATATATGTGACTTTGAAATCCTGGAATTCGTCCACCCCCTGTCCTACTTTAGTGAGACACAAGGTACACCTCTAATGTCCTCCCTTGTTGCCTTAGAGTAGTTAACTTTGAGGACAGAAAAAAGCATAGCCAGAAGATTGTAACTGAACCGTCAACTGTTCTGCCCTTGGAACATGCCTACTTTAAGCACACGTAGCTTTTTGTGTTGGGAAGTCAACTGTATGGATACTTTTCTGTTGACAAAGTAGCCAAAGACAATCTGCAGAAAGTGTTTTCTGCACAATAAAGGCAATATATAGCACCTGGTCTTTGTGGATTATTCTCTACACGGCTCCTTTTGGGAAAGGCTTTCTTCCCCAGATGTATGTTAGTCAGCTGTGAACGTTAATGTTGTAGCCAAATCAGTTGCAGAACTGAGGGTTTTCATTTTGTATACCTATGCAATCTAAGGCTAAAAAACCCCAAAAAACAAAGTGAAATGATGTAACTTTAAGTAACATTGAATCATAGATGAATATGCATGGCCAAACTGAAACCTGCTAATGTCCTAAAGTCCAGGATAGACGCCTGATACAAGCCACTGATACATAAATGAGTTGATAAAAGTTGGTTACTATTAAGTCTAGTTGCCATAGGTATCTGTCTGCCCATGGCTCTTGGACTCATTGGCTCTTTTCCATCAGTGGGGAGATGCTGCAGTGTCCTGGGGGTAATCTCTCCGGTGGTAGTATTCATAGTCTGACTCTAGTTTTGGTTTAATCTAAACCTCTGCTTTCTTAAGGCTGCAGATGGCCAGTTTGGTCATCAGTAAAGCAGGAGTCCTTCTTGGAGAATGTGCACACTAAGTATGAGATAGGAAGAGGCTGAGGCTTGGGGGACATCCCTCAGCGTTGACTTCTCCAGACCACATGCTAAGGATGGGAAGTGGCTGCTTTCTTGAAGACGTGGGATTTAAGTGTAAATGGCTGTGTTATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAATTTGCATCCATTTCTGAGAGGGCTGTGCTTACAAGCATCCTGTGGAACTCCCCATGTCCAGATGTTAGGTGAGTATTTTCAGAATTAATCCACTGCCAGAATTGGGAAACAAAACAGATGCTATGTTTGACTGTTGGTCCTCACCGGAGCAGGTGCTAAGATTGTGGGTATGGCCTTGAGGGATCCTGAGTAGGAGCAGGTGCCACATTGTGGCATCTCCCACCCTGTGATGGAGTCCCAGCTTGTGATCATGCTCTGACTATGTGGATTGGAGTGTGGTTTTGTATTCCAAGGACCGGACTGTCACTGTCCCGATGGTGTCTGCTGGGGCTGAAGGGCCAGCAGGCCATTCCTAGGGATGTAGGCTGAGGGGACTGAGCCCTTTTATCTTCTCTGAGATGAACCTTGTGTCATAGTGGGGCAAGTTCGATGCACAGCCTTGGATGACTCCCAGCCTGCTTGATGTCATAAACATATTTAGATTTCAATTATAGAAGTAGTATTTTAATAATCCAATCTTCCTGGTAATAAAGTGGAAACGGAAGGGGCCATAAAATGAGAAGAGCCTGGATTTGAGTACAACAATCTTCCTTCATCCACAGGCAATATGATGTAAGATCTGTTAGAAGACTGAAACATGGGTGGTGTGAGTACGTCTGGTTTTCCCCAATGCATCTGTCTATCCATCTGTCTGTCCATCCATTGATCCACCCACCCATCCAAAACTAGGCATTGCAAGAGATTAACAAACATAAAAATAAAAGTGAGCAGTTATCACAGTACATTATAATAAAGTCACCACCACTCACTTGCTGCATTGAGTGACGTTTAGTCATTAGTGCTGTGTTGGTATGTCGGGTAACCTTAATGTCTACACAGATGTGTAAGAGTGGGCTGGGTAGGGTGTAAGGCTGGGTTCCCTGGACAGAAGGAAGATTCATCCCAGTGTGATGGATCAGGCTTGTGCAAGATTGCTTTTTTTTATTTTTAATTCACTTTTATGATTATGAGCGTTTGACTTGCATGCATGTCTGTTTGCCATGTGTTTACAGTGCCCACAGCGGCCACAAGAGAGTATTGCATCTGGAATTGGAATTCCAGATGGGTGTGAGCCACCATGCAGGTTCTGAGAACTGAACCCAGGTCCTCTGCAGGAGGACTAACTGCTAAGCCATCTCTCCACCCCCAACCCAAGCAAGATCTTAATCACATTACTCAAAGCACCGAACAATTTAAACCTTATAAATGGTTTATTTCTGAAATTTTCCACTGACTCCTTTAGGTTGAGATTGATGTGGGCAGCGGCCTCTGTGACTGAGGGAGGAGAGCCATGTTAATCCGCTTTACTTGGTGGAGACCCGAGGAGGTTAAGTCTTCTCCCAGACTTCAGGTTCTTCACAAGTGACAGCGGAGAAAGTACTTAGCCTGCCTGCGAGAAGCGGGAGAGGTTTTGGAGCGCAGGCAGAAGGCCAGGTGGGCCTTGGTGTTGTAGAATTTTGACACCAGGACAACTGCAAGTCCAGCTTTGTTGATGGGGTCTTGGGATGAGATACTGCTGACGGGACTCTCAGGCCAGCGAAGCCACCTGAGATACCCAGGAGACTGACAGATGTCTGAGGCTCAGGAGGATGCTGGGAACTGAGCCTGCATGACCTGGAAAAGCAGCAGGTGCTCCTGACCCTGGAGCCACCTTTCTGGTCCCTAAACTTGATTCTAGAAACAAGGAAAACAAATTAGTGATGTTTACTAACATGGATAGAAAAATTCTTTTAACAACAATACAAAGACTGGCGAGATGGCTCAGCGGTTAAGAGCACTGGCTGCTCTTCCAGAGGTCCTGAGTTCAAATCCCAGCAACCACATCGTGACTCACAACCATCTGTAATGGGATCGGAAGCCATCTTCTGGTGTGTCTGAAGACAGTTACAGTGTACTCACACACATAAATAAGTAAATAAATAAATAAGTATTAATCTTTAAAAAAATAAGAACAATACAAATGAAAGCAAATGCGAATCAGACCACAGAGGGGAACCCAGGGACAAGGGCTAGGTGTGTTTTCCCTGCACAAGGATGAGGGAGAGGCTTCATCTGGTAGAATATTTTCTCTGCTGAGAGGTTTATCAGAATTGAGTTTTCAGAGCCTTATATCCAAGTACACTCTTCCTAAGCCTTACGTACCTCAGGGGTTTTGAGGTGTTCCCAAGCCTCCCTGCTGACTTTTACTTGAGAGGAATCCTGTCCTCTTGGAGACTCCAGTGCACCTGCCTGGGGCAGAGTAAACTCTTTCCAACAGCCCTCACCGGGATGTTTCAACATCCTGCTGCCAGCTCAAGAAACAGCTGAGTTGCTTTCTACCAAACTGTAGTGGCCTCTGAGTGTCAGTGGCTGAACCCCCAAACTATTCCTCTATGTGGCTGGTTTCTAGCAAGGTGCCCTGGAGTCTTTTGTTGCTGTTTTGTTGTGTTTGTTTATTTGTTTGTTTCAGGCACTCTCAAATGAATTTGCATTTTTATACCCTGAGACTTTTGATGGCAATCTATCCTCTCAATGATATCATTAATCTTTTTTTTAAAAAAGGATTTATTTATTTTATGTATATGAGTGCTTTATCTGCATGTACAACTGTACACTATAAGAGGGCATCAGATCCCATTACAGATGGCTGTGAGCCACTATATGGCTGCTGGGAATTGAACTCAGGACCTCTGGAAGAGCAGACAGTGCTCTTAACCACTTAACCCTATCATTAATCTTTTTAACAATTACACTTTTCTATAGTCTTGATAACTCCCATTTTCAAATTCTTTTGTTCATCTTTTCCCTCTTGATTCTCATTATAAACATGGCTAAAAGCAGCCTGCTGTAACTGTGCCCAGTTACTATCTTAAAACTTATGCTACCACAAATGAATCCGTTGTTTTGAATTCTGCCTCCCTTTTAAGACAGATATAAGGTAGCTAGATTCTATGTCAGGACATAATGTGAGTAGCCTGTAGTACAACTTGTAGTAGAGTCATGTTCCATCTGAAACCTTATGGGCAGTCTTTGCTACACATTGATGCTGGCGGTCTCATTACCCAGAACCCACCAAAATTTCCCATAAAGCTTTGTATCCAGCATTCCAGGGCTGTTCTAGTCTGCATTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTATATGTAAGTATACTGTAGCTGTCCTCAGACACTCCAGAAGAGGGAGTCAGATCTCATTACGGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCCTGACCTTCGGAAGAGCAGTCGGGTACTCTTACCCACTGAGCCATCTCACCAGTCCTAGTCTGCATTTCTTAAGTCTTCCATATTATTCTACAGGCAGTTCCCAAATGTCTATAGAACACACATTTAGGCTTATCACAACAAGAACAACAAGAACAAGAATAAGAACAACAACAACAACACCACTTGTAAACGCTTCCTGTATGAGTTACTCTTCTTGCTGCCATTTCAAAATATTGGACAGAATTACTTTAGAGAGAATAGGTTTACTTTGGATCGTGGTTTCTGAGTTCCTTATGGTGGGAAAGCATGGTGACTGTCATGTTTCAGTACAAGCTCATACTCTTCATGTGGCAGAAGGCTGGAGAGAATGCAAGCTGGAACCAGGGGTGGCTTTAGCCTTCAAAGCTTCACCCCTGCTGGCCTATTCCTATCAGCCAGACCCTGTGTCATAAAGTCTTCCCAACTGAAATGCCACCACCTTCTGGGGGACAAGCATTTCAACCATGAGCCTATAGTTACATCTCGGATCCAACTCAGAATGGTTGGTGATGGTGTGTGTGTGCCTGTGTGTACCTGGATATATATCCCTGTGTATACATAATAGTTTACATATGGGAGCCATACTCCTAGGTGGCTTCTCTTTGTGGTGGGTCCATTATAATCCATCTCAAAGAATCTGTAAGAGGCTGAACAAGCTACATATAAATTCCATTTATATATAAATATAAGTATAAATATAAAATTTTTTCCAGTGATCAGAGTAGTCATCTACGACATGTCTATGTGCTTGCTTGTGATCCCAACACCATAGCGGTGACCTTTGCCTTTCATTCCTTCTGTAGATGGTAATTTTATGTGGTGGCCTCATTCTCAATCTTTTTAAAAATTTCTTTGATGTATTATCTCATTTCTTGATACAAAAATCTCTACCTTATTCTACCTCTTAGTAACTTTGTTTACTAATATGTTAGAAGCCAAATTAAAATGATTTGCAACATTATTACATAAAGACTGGAGACGGTTTTTTTAAATCACCTTGGTCTGAGGATAATTATTTTTCAGCCCAGATTTTACCTTCTTCTAGCCAAATTTCCTTAGAAAATGATACACCTGAGATTTTCTGTCTAATATGTGGAAATGCCACTTTTCTGTTGAAGACTAAAATTTTGTTATGCACATTTTGTTGGTTTGTACTGCTGTGGTGAGGAAGTGTCCCAAACAGAGACAAAGGTCCCTCTGTGAGCCCTGTGGGTCTGGCAGTGCAGTGAGCACACTCTGTCATGTGGCCCTGGCTTCTTGCCCCGTCTGGGGTTCAGGCAGCCGCCTCCTGAGGCTGGCTCTGTGTGCTCTGTTCTTCGAGGCCAACTGTGGAAAATGCATGACATTCCTCATCTAGGTAGGAGAATGTCCTCCAGGACACACAGTTCAGAGGGCACTGAGGAAAATTCCTGTGTCCGAGTGATTGATGGAAGGCAGACCGGGAAGCAGTCTTGGATGCAGCAGCGATAGTATTTTTTGATTTATTTCTGGCTGGATAATTAAGCACGGCTTGCACGGTGATTTGTATTTGCTGGTTTGGTAAAATGTCTCTCACAGAGGCTGCAAACGAGCACTTGTGTGGCCTGATTTGATCTTTCACTTCATGTTGGGAGAAGGTTTTGAATAGTATGTGCACCTTCAAGAAGATGTCCAGAGACTAATATTTCTGTGAACTACAATGATCATAATTTGGTTGTATATTGTCATCTAATATATTGAGATGAGGTTAAAGAAAGAGCACACTTAAATGATATTATAATCAGAGATAATCTAGTTTTGTTTTTTCCCAAGAGTGCTACTAGATTGAGGACATTAAAAAGCAAAACAGAATTGCAGTAAACAAAGCTTACACTACAAACAGCGCTTACAGGAAAACTGTCAATTGTTATGTACACCTGCATGCCAGAAGAGGGCATCAGACTCCCTTATAGATGGTTGTGAGCCACAAGGTGGTTGTTGGGAGTTGATTTCAGGACCTCTTAACTGCTGAGCCATCTCACCAGCCCTGTAGTTTTAAATTTTATTATCTGACCAGTAATCACCTACTTTGAATGGTTTGAGTGTGGGACTTTGGAAACAGGTCCACACCGTGTGCGAAAGCTTTGCAAGGAGTGTATTGGCCTATGGTCTAGATTGCAGTGAATTGCTTTCTGTGCCTTGCTACATTTCTGCACCACTGAGGACAAGGACAGCTGGGTGCACATGTGTCTTCTATCTTTGGTGCTCATTTCCTTCATAGCCCATGCCTCTCTCTGACACAATGTCTTTCCATAATAAACACTTACCTTGAAGTCCTCTGATATTTCCAGAACAGCCCTTTGGCAATTTGATTTTCTATTTGCATGATACATTTTATATGGAGGGTACCTTTAGTATTTATTTTGTTACTTTCATTGATTTCTATGTTACTTTGTATTTTTTTCTTATATAAAATGTATTTTTCACTAAGTTGACTTGTGTTTGTTATTTCTTTCTATGAAATGCATATACTTCTTGTACACTGGGCATTTGTGGTTTTACTTCCGCAGTTCATTTTATTATGCAATTTTTGACAGGCCATTCAGCTTTGGTGAGGATAGTTTTATTCTATGCATCGATATCATAGAAGATATATTATCTAACTACTATTTCTAGTGTCATTGTGGTCTTTTGCATCTCCATTTGAAGTTTAGAATTAACTTTTCTATATGGACAAAGTAACCTGCTGGGATTGGATTGTTTTCTCTTTCAACATTTCAACAGTTTGGCTGGATATAAAACTCCACTGTTGCCGGGTGGTAGTGGCACACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGTAGGTGGATTTCTGAGTTAGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTACACAAAGAAACCCTGTCTCGAAAAAAACAAAACAAAACAAAACAAAACAAAACAAAACAAAACAAAACCAAACCAAACCAAAAAACCAACCTCCACTGTCCAGGAAGCGCGTGTTCTCTGGTGGGGCATCTGTAGATCTTGCTGCACTGATACACAGCAGGGAACATCCCTGGCCATCACTTTAGGCACAGGACGATAGCTCTTACAGGTAACTTAACAATTTTGATTAACCAATTTTTTTTATTCTTTCCAACTATTTTCCAATATTTTATGAGTTATTGAGTCATTTTTTGGTGTCTACTGTGGGCTTCTCAGTGTAAAGAGCACACTTTCAAAGCAGAGTATCTTAAGCTTGTAGCTGTGGTTTATGATTTCATTTCATGTTTTCATGGTCTTTGTTCTCTCTTTACTATACCAGTTGGATCTAGTTGTGAGAGCTCATCTTTTCCATTCTTCAGGTTTTTGGCGGTTTTGTTTGTTTGTTTTTTTTTTAAAAGACAGGGTTTCTCTCTGTAGCCCTGGCTGTCCTGGACTTGCTTTGTAGATCTGGCTGGCCTTGAACTCACAGAGCCTCACTTGCCTTTGCCTCCTGAGTGCTGGGATTAAAAGGGTGCACACCATGATCAGCCACTCTTCACTCTGTACCCGATGACTCTTCCTATCTACTTTACTTGGTTGGGCCCTTTCCCCCTTCCCTGCCTCAGACATTTACTGCACTTGAGCAGCCTCTCTCCCATCCTTTATTCTTCAGTTTTCTTTCTCTAATTAGGATGAACTGATGGAAGCCCCAGTGGTTTTGTTATTTTAAGTCTTTATATGTGGGTAAGGATGATGTATGTGTTGTGTGATTATGTGTGAATTCAGGCATGTGCATATGTGAAGTCAGAAGACAATACCAGACACCCATCTCCCCTTCTACCTTGTTTGAGACAAGGTCTCTTGCTATCAACTGTCACAGACATCAGGGGAGCTGTTTCTGATCTCCTCATCTCCATCTCCCATCTTACCATAGGAGTGCTGACATTACAAACATGCTACCATGTTCAGTTTCATGTCAGTTCTGGGGATTTGAACTCAGCTCCCCACAATTGCACACCAGGTACCTTGTACCTTACCCACTGAGCCGTCTCCCCAGGGCCATTTTTGCTAAGCTTGGAGCCATATAAGGATGATAGAGAAGGGATACAGAAGGGCAGACTCTCCCAAGTGTGCCTTAAAACTGTCCAGTTTGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGATTGCTCTTCCAGAGGTCCTGAGTTTAATTCCCAGCAACCACATGGTGGCTCACAGTCATCTGTAATGGGATCTGATGTCCTCTTCTGGTGTCTGAAGAGAGTGTACTCACATACATAAAATAAATAAATATATCTTTAAAAAACAAACTATCTAGTTCAGGAGTAGAGAAAATTAAACTTCAATCCATCTAACCTGCTTCTATTTTATCTGATGGATACGACACAATCTGCATCACCTAATTGTGAAATTTGCTATATTTGTAAAGCTTGCGGTAAAATATTTCTGATGAACTTAACTGGAGATTTGGGTTGATCAAGAAAGCAATGAGGGCAAAGAAGAAGAATTGCTAATAATTAAATAAAATGGCTCTGTATTCTGGGACTGATGGGGAATGGCTGCAGTCTAAGTACATTCTAGAAAATCCTTGGTGGTTTTGAAAAATATTAAGAGCTGTCTACCTCATGGCTCATTTTCTCTATCTACTCTTAGATATTGTCCTTCTTATTTATTTTATTTTTTTTCTGTCAACTTGACACAACTTAGAGTCATTTGAGAAGGGGAAACCTCAATTTAAAAAGATGCCCCCACCAGACTTGTCTCCCCAAGTTGCTTGGGGTTGTGGTGTTTTTTACGATAGTGATACAAACCATAATTAAGAAATAAATTGAATTGGGCCCTGCACCTTGCCTGGGCAACACAGTGGAGCTAACCCTGGTGTCAAAGGCATGGGTGAGCCAGCTCTGATGGTGTGAACTAGAGAGGTGGCTCAGCCCTTCACAGGCTGCAGGACTTGGGAGAGTGGGTCCCATGCCTTGACTGGGTTGCACAGTGGAGCTGGCTCTGAAGGTGTGGGTGCTGGTGAGGGCATGAGACCAGGAGAGCTGGCCCTGACTTCTGCCAATGGTGGTGTCTTAGTCAGGATTTCTCTTCCTGCACAAACATCATGATCACGAAGCAAGTTGGGGAGGAAAGGGTTTATTCAGTTACACTTCCATACTGCTTGCTGTTCATCACCAAGGAAGTCAGGACTGGAACTCAAGCAGGTCAGGAAGCAGAAGCTGATGCAGAGGCCATGGAGGGATGTTCCTTACTGGCTTGCTTCCCCTGGCTTGCTCAGTCTGCTCTCTTATAGAACCCAAGACTACCAGCCCAGAGATGGCCCCACCCACAAGGGGCCTTTCCCCCTTGATCACTAATTGAGAAAATGCCTTACAGCTGGATCTCGTGGAGGCATTTCCCCAACTGAAGCTCCTTTCTCTGTGATAAGTCCAGCTGTGCCAGGTTGACACAAAACCAGCCAGGACAGGTGCCCTGGTGTTTCAGGTGCAGGATAGGTCACTGGCAGACTGACCAACTCGGCTACCACCCAGACCCAGGTCCAGGGCTTTGAGTTGGCCTACCCCCAAAACTACCTGGCTTCTGTAACACCATCAGTATTGGATCCTCGTTGGGACTCCTCCCAGTTACTTCAAACACTCTGAATTACTCATACATTTTGGCACTGGAGGCTGGAAAGATGGCTCAGTGGCTAAGAGAGCTTGCTCTTCTTATCAGGGACCAAAGTCCACTTCCCAGCACACACTGTGAGTCCAGCTCTGGGGGACCTGACACCCTCTCCTGGTCTCCTCCTCAGGCACTCTCATGCATATGCACATGGAAGCACTCATACACATAAGCAATAAAATGCATTCTTAAAAGTACACAAATATGGGGATGGAGAGATGGTTCAGTGG
GF3 -1 -1 1 CTTGGCATACTCTCCTGTTATCTCCGCTG
GF4 -1 -1 1 GCCTGGAACAGTAGTGCATATGACGACAG
EX52 -1 -1 1 GAAAGGTGAGAGTTCTAACCTTGCTCCTG
EX32 -1 -1 1 CGTACATTGGGAATACCCCGGCTG
GR3 -1 -1 1 GCTGAGACCGTGTGCGTCAGTGGTG
GR4 -1 -1 1 GACGTGGCAGCTGAGACCGTGTGCGTCAG
Comments:
Created
Target: 2803Ensembl ENSMUSG00000062327 -- 8628976 -- 8630076 ENSMUSE00000496630 -- ENSMUSE00000496979
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: 17, start 8628976: end 8630076
Found 10 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
REJECT (1) gap pair G5:17:8622476:8623475_946 G3:17:8633576:8634575_765
REJECT (3) gap pair G5:17:8622476:8623475_946 G3:17:8633576:8634575_172
ACCEPT (0) gap pair G5:17:8622476:8623475_946 G3:17:8633576:8634575_160
ACCEPT (0) gap pair G5:17:8622476:8623475_842 G3:17:8633576:8634575_765
ACCEPT (0) gap pair G5:17:8622476:8623475_842 G3:17:8633576:8634575_172
ACCEPT (0) gap pair G5:17:8622476:8623475_842 G3:17:8633576:8634575_160
REJECT (2) gap pair G5:17:8622476:8623475_665 G3:17:8633576:8634575_765
ACCEPT (0) gap pair G5:17:8622476:8623475_665 G3:17:8633576:8634575_172
ACCEPT (0) gap pair G5:17:8622476:8623475_665 G3:17:8633576:8634575_160
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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