High Throughput Gene Targeting: Create Design

This custom vector design tool is provided by the EUCOMM consortium as a service to the research community. When using this tool, the user agrees not to submit more than five designs per day for creation without prior discussion with the High Throughput Gene Targeting Group at the Sanger Institute.

Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by am9
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 96792
Chr: 7
Strand: -1
Status: Ordered
BACs: RP24-456L5 : RP24-363C7 : RP24-331L16 : RP24-349C24 :
Instance: Plate 101: Well F06: BACs RP24-363C7 RP24-331L16 RP24-349C24 RP24-456L5
Instance: Plate 210: Well G07: BACs RP24-363C7 RP24-331L16 RP24-349C24 RP24-456L5
Oligos on GRCm38:
Type Start End Length Seq
G5 131333632 131333681 50 TGGGAAAGATGTATACTCCCTGCTATTGTATGTTAGAGGTTATTGTTTAG
U5 131327677 131327726 50 TGCCTGGACCTCCTGAATTATGCTCACATTCAGAGTCACAACCTTCTGCC
U3 131327627 131327676 50 CAATAATAAAATGGGCCTACCCTGGAACTAGTAGCTGATTGCTGCACACA
D5 131326025 131326074 50 TTGAGTAAGAAAATATTCTAGTAAGAAATTGAAATACTGATCATGAAGAT
D3 131325975 131326024 50 CATAAACACAATGTTAGATAGTATACAACATAAACACTATAGAAATTGGC
G3 131321932 131321981 50 GTTTGAGTTGAAGAATCTGGTTCAATTTTCTTACCTTCAGCCTCTGTAGA
Oligos:
Type Start End Length Seq
G5 138477146 138477195 50 TGGGAAAGATGTATACTCCCTGCTATTGTATGTTAGAGGTTATTGTTTAG
U5 138471191 138471240 50 TGCCTGGACCTCCTGAATTATGCTCACATTCAGAGTCACAACCTTCTGCC
U3 138471141 138471190 50 CAATAATAAAATGGGCCTACCCTGGAACTAGTAGCTGATTGCTGCACACA
D5 138469539 138469588 50 TTGAGTAAGAAAATATTCTAGTAAGAAATTGAAATACTGATCATGAAGAT
D3 138469489 138469538 50 CATAAACACAATGTTAGATAGTATACAACATAAACACTATAGAAATTGGC
G3 138465446 138465495 50 GTTTGAGTTGAAGAATCTGGTTCAATTTTCTTACCTTCAGCCTCTGTAGA
G5_U5 138471191 138477195 6005 TGCCTGGACCTCCTGAATTATGCTCACATTCAGAGTCACAACCTTCTGCCCCCGGAGTGTGAGCCTCAGAGGTCTTGTTCAAGAATGACCACTTCTAAGACAGAACTCCAACAGTCTGAGAACCTGAAAATCCATTTTGTAAATGCAGTTTGCTATGGGCAGTGGGGGTGGGGGTGGGGATGGGAACTCAAGTGTTGAAAGCTGTTGTTTTCTTTTAAGACCTAAATCTTCAACATTTCTCATTTGGAATAATCAATTTCTTGAAACTTAACTCTCTGACACTCTCAGAGGAAAAGTGATGAGGGTGGCAGGCGGTGGGGAGATGTTCTAGTCACGGAAATCCACTTGCTTTCCGATTCATTGCTGGGGGAAAGCCAACACTTTTAAAAAATACAATTAGACATCAATTAAATGAACAGAATAAACCTAAGTCAAGCATGGGTTAACTCCCTAGGGTTACTGAGGAGGGAGGTCCCAGAAAACCCCAAAACCTTTTGTTATCAATCATAGTTAGATGATAAGGCCTTCTTGCTGAAGACAGCACACTCTGGTTGCACAGCAGAGAAATCAATTTCAAAATGATCAGGAAACTTTCTCCTGATGACTAGCTCTCTTGGGGCCTGAATGTGTTGTACAGACCTCATAGGGAGAGAGAGAGAGAGACAGAGAGAGACAGACAGAGACAGACAGACAGACAGACAGACAGACAGACAGACAGAGACAGACAGAGAGACAGACAGAGAGAGAGACAGACAGAGAGAGAGACAGACAGACAGACAGATCAGAGGTCATACTCAATGCTGGGCCCTGTGTACTACAACACTCACCTGCCAGGCTATTATGTATGAGGCAATGGAGGCATTACTGTTTGGTGTGTGGGGTGGGGGTAACCAGTTACTTTCTGACTGGATTTGAGGCCTGCTCCACAAGAGGGGATCCATGTCTGGTAAACCTGGTCAAAGACCTTGGTTGAGGAGGTCTAGGCATTAGGAGGGAACCCATACTCTAATTTTGATAAGTGGTCATGTCAAACTGCCTTTTCATGTTTATATTTATACCCATAGGTCAATGCTGGTCTCCACTTTGGCCAGGGTAGGTTCTCTTCGCAGTGGGCAGTAGTTAATGCCCACACTCATAACTGTTAAAGTGCTAAGAATAAGTGACCGTGAGTGCTAAGGCATAGATGGGACACATATTATCAACTTTATATCCCCCAACACTAAGGCTCAGGATGCATTGTGAAAAGGGGGAAAGAAAGACTCTAAGAGCCAGAGGATGTGGCATGAAGAGCACTGTGTAATGTTGTCTTGTGGCCATGGTTTCATAGCAGCTGTGATAACCTATACAAGATCAAGCCAGTTAAAATTTCAGCATATACAGAGTAGGGCCTCCTGAAGCCCCACACCTAGACAGCAGCTACTGGGGGGGGGGGGGTTCTGGGAAAAGAAGACTCAATTTTGGGGGAGATATGGCCACCATGAGCTTGCCCCATGCCATGGTGAATGGCATCACACCTATGTGCATATAGACAGTACTTACTGGACTCAGTAGGTTATAAAAAATTTAAAAAAAGATGGCATGAGAGTAGAAAAGAGATGTCAGGAGGCTTTCTGGGTATGAGAGCTGAAAGGCAGAAGGGTAGACATGATCAAGATACATTGTATTCATATGTGACATACATTAAAATTTTAAAGAATTAAAAATGTTACTCAAGACAGAAGGAAAATTGTAATTACTAAGGTCTAAGTTTGAAGAGCTTACTTTCAGAAGGGCGGCCTGCTGGAATGGATAGCCATTGTTAGCTCTTATGTCTCCCCTAGTCTGAGGCACATGCTGTGTGACATCAACTCTCTAGATCTCAGCGTTCTTTCACAGAGTTCTGAATTCCGTTCCATGCTCATCTAAAAGCTCATCACAACTTGCCCAAACACATGGGAACAAGTGCAATTCCTAATTCTCCCTTTTAGCCTCTGGAGAAATTTAGAATTTGACTGTTAATCGAAAGTAAAATTGGAGTTTTAAAAAAAAGAGAGAGAGACATAAGGTGAAGAAGCCAATGTCTTAGAACGTTCATTTCTCTAAAAAGCCATTAATAGAAGACTGGCTAGACTCTGTATTTTAGCTACACAAATATGGGGTCATGTAAGAGAACTCAATCATAAACCTATTGGCATCATATCATACTTTATGGTTAAGATGAAAAGATTCCACAATGCTGATACATATAAGACACCTGCTTTAATCGTATTTCTCTGCCCTCAAGGTATTCCAAACCAGAAAGGCCAATGCAACAGGTTATTCCTTGCTGCTCTGGGAGAAACCTAAGGGAGCCAAGAATATCAGTGACTATGTCAGCCTTTCCAGGCATAAGAAATGCCACATTCACAAGAGCTGGCGAATGGCATCAGCTTAGCTATGCATCCACTGATGATGGAAAGATAAAAGTGCAGTGCAGCACATGCGCAATAGCACTCAACTGCAAAGGAAGGTGATAGGAAGTGCAAGAGAACAAGAGGAACTGGAAAATGTTATACTGGTGATATAACCTAGGCCCAGGAAGATAAACTGCATTTCTCTCTCATATCTGGATCCTAGCTTAAAATATTTAGATCTGTGTGTTTAACTTCAAGTATATATGTTCACATAGCCAGAAAGGGCCCAGGAGAGAGGAAAGAAGCAGCCTAATAGAGGGGGAGCAATGGTAGAACACGTGATATTAAAACAGGGGGAAGGGTACTGTGGGTTAAAGGCAGGGCAGGTAAAGGAGGGAGAGGAGAGGAGAGGAGAGGATGGGGGGGTTAGTGAAAAAAGCTGCATCCAAGTTGTTATGGAGACCCACTGTGTGGGTGATTTTAAAAAGTGTGATTCTGGAGGGCTTAGAGAGTTAAGAGCACTTGTTGCTTTTGCAGAGGACCCAGGTTCTACTCCCAGCACCCACATGGCAGCTCACAGCCATCTATAACTCTAGTCCCAAGGGAACCTATGCCCTCTTTTAACCTCCCTGGACATTAGGACTCCACGTGCTACACATGCGAATATGCAAGCAAAACACTAACACATGATAAAATAAATAAATCTAAAATATTAAGTATGTAAGATTTTAAGGAAATAAAAAAGTTGAAACAGATCTGCAGCATGGGTAGATAATGCTCCTCCCAGAAAACATAGCTTATCAAATGAAAAATCTCAATGCCATGCATGGAATATTCAGCCAAAGAAACAGACTGTTGGTTGGACACCATAACTGGATGGTAAGACTCTACATCCAAAGATGCCACAGGTTTTGTTTTCAGAACATGGAGAAACCAAGTTGGAACTGACAGGAGTATTTTCCCCCTCCACTGCTGGCTAGCTTACAAGTACCTGAAGGTGCTTTGCAGGCCTCTGGGGTAGAAAAGAGATCAAAGAACCCTGAATACGATAATACTAACCTGCCAGGCACCATGTACCCACTAGAGCAATAGTGACATGACTGTTACAGGGAGGGAGGTAACCAACTGCTTTCTGATTGGAGTTGAGGTCCACTCCACAAGAGAGTCCATGCTTGGTACTGCAAGCCTGAGAAAAACAAAAACCTTGGTCACTGTCCTGGTTTCCTTTTCTTTTGCTGTGACAAAACACTGACTAAAAGCAACTCAAAGGAGAAAAGTATTTACTTAGATTACAGGTTATAACTCACCATCAAGGGATACTGAGGAAGGAACTCAAGGCAGAACTGAACATGGAGGCAGGAACCAAAGCAGAGGCCATGGAAGAACACGGCTTACTGGCTTGCTCCCCATGGCTCCCTGAGCTTGCTTTTTATAAGACTCAAGGCCACCTGCCCAGAGGTGGCACAGTCCACAGTGTGATGGGAACTCTCAGATCAATCATCAATCAAGACTATGCCCCTCCCCCTACAAACATGCCTACAGGTCAATTTGATGGAGGCATTTCTCAACTGGATGTTCCTTCTCTCAGATGACTCTGGCTTGGTGTTAAAAATAAGACTGAAAGCTAAGCACAACAATTATCCCTAGAGTGTAAACTGACGACATGAACACTTCATGATGTGATAAGGGCAAAGTTTTTTATTGTAGGTGAGAGAGATAACAAGCAGGAGTATCTAGGAGAGTTCAGGGCAGAGAGAGTAGACTGAGCTTGGCCAGCAGACTAGACCTGCTCATAAGAGAAACAGCAGAGCAGAGAGAGAAAGGGGAACAGAGGTAGGGCATGGGATGGCAAAAAGAGGAAGAGGAGGGAAAGAGAGCAGAAGACAGAGAGAGCATATATGAATTAGCAGGGTTATAAGGAGGAATAGCTGGGAGAAGGGGAGCCCATGACTGGGGGAGTTGAGGGTAGGGGAGGAGGTGAGAAGAGCTAGGATGCTAGCATGAACTTCGAAATGTATAACAGGTACTTGTGACAATGAGGGATCCCTGAGACCAGCGTGTGCTGTGGTGTGCTAATAGGCTGCACACATTGTCACTTGTCTGTCTGTCTGCCTTTTGAAATTTGGGGGGAAGGAGTTCCCTTTGAACCTGAGACCTTGGTTCCTAAATGATATGGATCTTGGATAGCTCTGACATAGTCCTAGGAGTCCAAAGACCATGCACATGTATAAAGCTGTGTGCCTAAGGAGGATTATAAACACACCTAGAAAGTATGAACTAGCTAAAGTACTCCATCTGGAGGATGCTGGAGGCTCTTCCCAGTGAAGTGGGGGCTGAGATTTGGTTCTCAGCTGTTTGGCTCGTTGCTGCAGACATGGTCCAGGGGCACCCTGAGCCCCTCAGCAAAGACTGGGAGATTTTGTTGTTCTTGTTCCAGAAATTTAAAGAAATCTTTGTCTACTAACCACCTCTAGGCAAATCAGTGTCCACATACGACAAAGGACACAGAGAGTCAGCTGGGGAAAGTCACTAAAGATACCACATAACTCCAACAGGCACAAGAGTCCTGGGGAGACAGAAGACCCTGATCTTCAAAGACCCCACGTTGTACCATTTAAAATATCTGCTTCTCAGGCTGGGAGATGGTGTACATGCTTTCAGTCCCAGTACTGGGGAGGCAGAGGCAGTGTGAGGCCAGCCTCAAACTGCTCTACAGAGTGAGTTCCAGAGTAAATTCCAGGATAGCCAGGGTTACACTAAGAAGTCTTGTCTTGATTAAAAAAATAAAAACAAAAAAAAAACAAAATAAAAAGTAAACAAACAAATAATCTAGTTTTCAAAAATGACTATAAATCATTGAGAAGTAAGGTCTACAAAAAAAAAATAGAACAATCAATCAAAATGCTATTTGAAAAGGCCACGTAAGATTTTGACTGTATTTTGACAAAGATTTAATTTGGCTATTTTAAAATTATGTTCAAAGAAAGCCATGTCTAAAAAATTAAGAGAAAATGTGTTACTATAAAGAATATTTATTTAAAAAAAGGAAACTATAATGAAGAGTCAGGAAAAATTATAGAGTTGGAAACTATAATAAATAACATTAAGCATAATTTAACAGTGAATGTGAGCAGGCAGAAGAAAGAATCCATGAACTGGAAGATGATGGGCTATCTGCGGGTATGAACTGGAAGATGGGCTATCTGTGGGTATGAACTGGAAGATGGGCTATCTGTGGGTATGAACTGGAAGATGGGCTATCTGTGGGTATCCAGAGGAAAGAAAGAAATCAATCCAGGGCTTTCCGTTTGTTCCAATTGTGTGAAGAGCTTGCGTCATTCCCATGCTCACAACAAGAAGCTGAACGAACAGAAAATCAGCAATTCTATAGAAGAATGCTATAGAACTAAAGTCAGGACAGACAGCCACCCCCTAACCCACGCAGACAGATGAACAGCAAGCCAGTTTACCAGCAGCAGAAACCCTCCCAAGAGGCAGCAACAGTAAGAGTCCCAGATAGTCATTATCATATTTCCTGGGGCTAGGGGAAAAGTTTTGAACTCTTGGATATCCAGTCTGAAAGTGGGAAAGATGTATACTCCCTGCTATTGTATGTTAGAGGTTATTGTTTAG
U3_D5 138469539 138471190 1652 TTGAGTAAGAAAATATTCTAGTAAGAAATTGAAATACTGATCATGAAGATTTCCTTTTATCCATAGGTGCCATGTCAGAGTCCTTCCCTTATGCTACAAAGGCAGGGTGGCAGGGAGTTAACATCTGCATAGACACCACATACATTACAACACTGGAGGGGACCGCAACTCGCAACAGCAATGGTGTTGGCCTGGTAGGAATGACAAGCCTCAGCAGGGATGCTGTTGGCCAGGATGATGCTCTCATGGGGGTCCTTGCATGGATCAATATAGCATTCATCATCTGCCTCCTTTGCGATTCTTAAGAAAAGACACAAAACAAGCTTAGAATTTCCCTTCTCAGTGAGCTTCTTACAATTATGAGGGCTTTGTGAGAAAGGCCAGGGTTGGAACCCATCATACATGTGACACCAGCCCTGGGTTAAAGCTCTCTGCTCCTTTCCCATCTTTTCCTCTCCTACAATTGCTATGCATGTGAAACCCAGATTCCATCACAAGTAAAGAGAGTGACTGTGCCCCAGGGGCTCCTGCAGGCCCAGGAGGATAATAGAGAAGGCACAGCAGTTGGCTGGGCTCAGCTCTGGCCCCTACAATCTAAGTCCCTCTTAAGGGACATCATGACAGCCAGTTTTTCTCATACTTAAAGCTTCTCTCCCAAACTTCAAACCATCGCTTGTCTGACCTACATCCCTGTCAGCTGTCCTCCACTTTATTCTCTTGCCCCATGTCACTTTCCCCAAAGTCACTGGCACTGTGGGAACATGAGAGCTCCCCAGTGAGTGGAGAAGAGCTCACTATGGCCAAGCTCTGTGTGAATAGGAGACAGCTCATCCCCAGGGCATCCTCTGGCTTCTCCAGTCCACAGTGCTGAGGTAGAAAGGATGCCCAGCCAGGGCCCTGAGAAGTAGCTGAAGGTTCACTCCGTGTCTAAATGACAGCAAAGTGATGCTGGCGCCCATGCTGTCCAGGTTGAGAGCAGTCAACTTAGGAGCTCACAGCCCCAAGCTGGAAGGAGAACCCTCAAACTTGTGTGCCCACCTGCCTTCTCTCTCAAACCTGACAGTGGCTCTTGACTTCCCCCTACAAGTGGCTACTTCAGTAAGAGGTGACCCAGCTCCAGCAGAGGGCCCCAGTGCCCCATTAGAGGAAGGTATTGTTCATTCTTACCCAGGGGACTTCAGAGCCTTGGATATTTTGGAGTAAAGACAAAGCACAAGTCCTATCAGTAGAAACATGGCTGTCAGGATCCCGCAACCAATGATGATCGTGATTATTGTCCGGAGGTCAAAAGGCTTATACATCCCTGGGCAGAGAAGTGCAGACGAGCCGTGAATGAGGTGTCCCTCCTACACACCCACAGCAGCCTCCTCACACTTCCTTCAGTGAGGGGGAAATGAACCTCAAACACACACACACACTTGCCCAGCAATCAGATGAACTCTTTCCAGAGAGGTCTTACCTGGCCCAAGGTAGGTTAGAAGGAAAGTTTTCCGCATAAAAGGGGATTGAGATGTAAAATCCAGAGGCATGAGAGCCAAGGGACATTCTGAAGATAGGAGGACAGGCAGAGGAAGGCCAAGCAGGCAGAAATTAACAGACACTCAATAATAAAATGGGCCTACCCTGGAACTAGTAGCTGATTGCTGCACACA
D3_G3 138465446 138469538 4093 GTTTGAGTTGAAGAATCTGGTTCAATTTTCTTACCTTCAGCCTCTGTAGAATTCAGCTGTGCCCCACAGGAGACAGCTAAAATAGCCCAGATGAACAGCCTTCCAGTGACCTCCATCTTCGCAGGGTGCCTCAGGGCCCTCACCCTCAGGCTTCTTATAATTCTCCGAGTTTTGTAAGCTTCCTGGTATCTAGGGAAACGTGTCAGTTTGGCCATCCCAGGGGTGGTGCTGGTGAGAACTCCACAGCTGAGGAGCTGCAACCTGGCCAGCAGTTCCTGGCACTGGGCTCAGTCTTCCAGGTCACATCTCAGAGGGCTATAAGCCAGCCAGCCCGATTCTGCACAGGGCTCAGCAGCTACGTAGCAGTTTCTCTGGCCTTGAAACTGAATTTGAGCCTTGACCCTGTCTGTGCAGGTCATGAATGAAGGAGCAATGCACCAGGATCTGAAGCTCAATGAGCCAGGGCATAGCAAACTCTTGGACCCAGCTGGGCCCTCAGATGCCTACAGGAGGCTGACCCAAGGGGTAGGGGAGGGACAAAGGATGTGGAAGAAAAGACAATGTAGATCAGATCTCCCTGCAACCCTGCAAGGATTCCTGACCCCTGTCCCTCTGAAGAGGCTAACATGGATGGATTAGAATCCCTCAGGCACTGAGCTTGTACCTCAGAGATAGAAGTCCTAGTGTCCAACATGAGACTAGGGAGAAGAGTGGGCATGTGAAAGGGACTGTCCATTCACTCATTCATTCATTCATTCACTTATTTATTCATTCACTGAGCAAGTTTTCATTGCTCACTCTCCTAGGATGTGGGAATACAAAGCTGAGTACAAGATACGGAGACTGTACTCTTATGGAACCTTTAGTCCAAGTGGGGAAAACATAATGAATTAATCACAGTTCATACAGGGGCAAGTGTGTTGAGTGTGCTACTGGGAGATCGGATAGAATGTGGCAGCCTTGGGTGGGAAAGAATGCAAATTAGGGCAAATGAAAGGCATCTCGAGAAGATGAAATGGAGGCAAGAACAAAAAGAGGGAGACAATCATGTATCTTACTTGAGGAATTTTGTTTAAGATAAAAGTATGTGTATAAAGGCCCTGGGTGCAAGAACTTCTACGCACTGACTACGTGTGAAGGAGAGGGAAGTGGGGAGGCAGGCAGAGGAAAGGTCAGGGGACAGTTGATGGAGACAGACCTGTGTGGCAGAGAAAAGCTCAGTCTTGTTCTAATCTCACGTGGAACTTTTCAGTGTATGGCCAGCTCTAGGCATAGTGGCTACAAATGAGAACGGGCCCCATAGGCTCATGTAATTGAATTCTTGGTCCCCAGATGGTAGAATTGTATAGGAAGGATTAGGAGGTGTGGCCTTGTTGGAGGAAGTGTGACACTGAGGGTGGGCTTTCTGCCTCATGCTTGTGGATCAGATGTAAGCTCTCAGCTTCTGCTTCATTGCCCTGCCTGCCTACAGCCATGCTCCCCACCATAATGGTCACTGACTCACCCCCTGAAACTGTAAGCCCCAATCTTCTATAAGTTGCCTTGGTCATGGTGTCATATCCTAGCAACAGAAAAGTAACTAAGACACTAGGAATCACACAGGAGCTCCTTGAAGATCAAGGTGAGGCAAGGTCACCTAGAATGGAGCCTTCTTTCCCTCTCTTTAGAAGGCACCCCTTTAGCTCCTTGGGTACTTTCTCTAGCTCCTTCTTTAGGGGCCCTGTGTTCCATTCAACACGTGACTGTGAGCATCCACTTCTGTATTTGCCAGGCACTGGCATAGCCTCACAAGAGATAGCTATATCAGGGTCCTGTCAGCAAAATCTTGCTGGCATATGCAATAGTGTCTGGGTTTGGTGGTTGTATATGGGATGGATCCCCAGGTGGGGCAGTCTCTGGATGGTCCTTTCTTCTATCTCAGCACCGAACTTTGTCTCTGTAACTCCTTCCATGGGTATTTTGTTCCCCATTCTAAGAAGGAACAAAGTATCTACACTTTGTCTTCCTTCTTCTTGAGTTTCATGTGTTTTGCAAATTGTATCTTGGGTAGTATAAGTTTCTGGGCTAATATCCACTTATCAGTGAGTGCATATCATGTGTGTTCTTTTTTGTGATTGGGTTACCTCACTCAGGATGATATCCTCCAGATCCATCCATTTGCCTAAGCATTTCATAAATTCATTGTTTTTAATTGCTGAGTAGTACTCCATTGTGTAAATGTGCCACATTTTCTGTATCCATTCCTCTGTTGAGGGGCATCTGGGTTCTTTCCAGCTTCTGGCTATTATAAATAAGGCTGCTATGAACATAGTGGAACATGTATCCTTATTACAAGTTGGAACATCTTCTGGGTATATGCCCAGGAGAGGTATGGCTGGATCTTCTGGTAGTACTGTGTCCAATTTTCTGATTTCCAGAGTTGTTTTGCTTTTTTATACAATATTTGTCCTCAAATACAACTTTGGTAAATAAATAAATAAATAAATAAATAAATAAGCAACATAGTCTGAGATAGAGATCGAGGGCATTACTAGAGAAGGAAATGGCTTTGATGTCCATAGCAATAAACTGTTCTGTCCGTGGCCATTTAAGCAGACAACCCAGGCATAACAGGACAAGGGGTTCTGGAGGTGGAGGCTGACTTAATTGTTCTGAGAAATCTTGCCAACTCATAATGATTAAGTTGTACCAGGAAACATTTGAAGCAGGAAATGTTTTTGAGAAGCCTGAAGTTTTGGTAGTTAAGAAAACTGAAGATCCCCAAGGGAAGGGAACAGTGGGCCTGGGTCACCAAGGACTGGGATGTTCATTAAAAAATACAAGGGACAAGAAAGAGGAAGACATAGCTGGGTCCACTGGATTAGTGAGCAATGGTGAGAGCCCCCACGAGTGGAGATCTCTAATAATAGAAAGTTTGATCCAGAAATATAATTGGCGAGGACAGGAAGATATATGTATAATAAAAAAAGATAAAAGGTTGGGTAGGAGAGTTTGGAGCCCATGAGGGGCCATTGGATTAGTGGACTGACTTTCTGGGGAGACCGGGACCTACTGATCTGATCCCCTGGCATTGTCTGCCTAAGGGAACTAGGGTCCAAGTTCTAAGAGACAGAAGTCAGGCTGGGAAGGATCTGTGGGGGAAGCTGCAAGAGGAATTAACCTTGCATATGGAAAGGTTTGAAAAGCACTGCACAGGTAGACATTTGGATGACTTAATAAAGAGATTCTGCCGATGTAGACAATTAAAGGCTAACATTACCCTGACCTGTTTCCCATATTTCTGTTCTCAGGCTGATACTGTAACCGCACATTGACGACTTGTGGTTTGAAGACTTTTCTGAGAGCTAAAAGTTAATATGTAACATGCTTTACCATACGCTGAACTGTGACTTTGTATGTGCATTGTGCTTTCTGGTGACACAGTCGCATGGGGTTTTTTTTGGGGGGGGGGGAATAACAGGCTTGAGGAAAATAATGAAAAGGCTTTTGATTATGTATTATTATATAACAAGGACATGTGCAACCAATAAAAGACAGAGGTGGACCCAGAGAGGAAAAAAAAGGACAAACCTGAGAGAGAGAAAAATCTGAAGGAGGGAGGGAGAAAGGGAGGAGGAGGGGAGAAGGGAGGGGAAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGCGAGAGGGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGAGAGAGCTGTCTCTGAGCTCAGCTTCAGAACAGCAATGGTGACCTGTCAGCTTGCACCTGCATTTGTGCCTGAGTCTCTGATTTTGTGCCAATCCAATCCTCCCATTCTTCAGAACTCCAAAATGACTGAGGTTGGTCCTCGGTGGTAATACATATAGTGGTATAAGAAATATAACTAGGAAACATAATAGAGGCAGAGATTGTTTTCACTATTGTACATTTATACAAAAGTACATAAACACAATGTTAGATAGTATACAACATAAACACTATAGAAATTGGC
U5_15 138471191 138486241 15051 TGCCTGGACCTCCTGAATTATGCTCACATTCAGAGTCACAACCTTCTGCCCCCGGAGTGTGAGCCTCAGAGGTCTTGTTCAAGAATGACCACTTCTAAGACAGAACTCCAACAGTCTGAGAACCTGAAAATCCATTTTGTAAATGCAGTTTGCTATGGGCAGTGGGGGTGGGGGTGGGGATGGGAACTCAAGTGTTGAAAGCTGTTGTTTTCTTTTAAGACCTAAATCTTCAACATTTCTCATTTGGAATAATCAATTTCTTGAAACTTAACTCTCTGACACTCTCAGAGGAAAAGTGATGAGGGTGGCAGGCGGTGGGGAGATGTTCTAGTCACGGAAATCCACTTGCTTTCCGATTCATTGCTGGGGGAAAGCCAACACTTTTAAAAAATACAATTAGACATCAATTAAATGAACAGAATAAACCTAAGTCAAGCATGGGTTAACTCCCTAGGGTTACTGAGGAGGGAGGTCCCAGAAAACCCCAAAACCTTTTGTTATCAATCATAGTTAGATGATAAGGCCTTCTTGCTGAAGACAGCACACTCTGGTTGCACAGCAGAGAAATCAATTTCAAAATGATCAGGAAACTTTCTCCTGATGACTAGCTCTCTTGGGGCCTGAATGTGTTGTACAGACCTCATAGGGAGAGAGAGAGAGAGACAGAGAGAGACAGACAGAGACAGACAGACAGACAGACAGACAGACAGACAGACAGAGACAGACAGAGAGACAGACAGAGAGAGAGACAGACAGAGAGAGAGACAGACAGACAGACAGATCAGAGGTCATACTCAATGCTGGGCCCTGTGTACTACAACACTCACCTGCCAGGCTATTATGTATGAGGCAATGGAGGCATTACTGTTTGGTGTGTGGGGTGGGGGTAACCAGTTACTTTCTGACTGGATTTGAGGCCTGCTCCACAAGAGGGGATCCATGTCTGGTAAACCTGGTCAAAGACCTTGGTTGAGGAGGTCTAGGCATTAGGAGGGAACCCATACTCTAATTTTGATAAGTGGTCATGTCAAACTGCCTTTTCATGTTTATATTTATACCCATAGGTCAATGCTGGTCTCCACTTTGGCCAGGGTAGGTTCTCTTCGCAGTGGGCAGTAGTTAATGCCCACACTCATAACTGTTAAAGTGCTAAGAATAAGTGACCGTGAGTGCTAAGGCATAGATGGGACACATATTATCAACTTTATATCCCCCAACACTAAGGCTCAGGATGCATTGTGAAAAGGGGGAAAGAAAGACTCTAAGAGCCAGAGGATGTGGCATGAAGAGCACTGTGTAATGTTGTCTTGTGGCCATGGTTTCATAGCAGCTGTGATAACCTATACAAGATCAAGCCAGTTAAAATTTCAGCATATACAGAGTAGGGCCTCCTGAAGCCCCACACCTAGACAGCAGCTACTGGGGGGGGGGGGGTTCTGGGAAAAGAAGACTCAATTTTGGGGGAGATATGGCCACCATGAGCTTGCCCCATGCCATGGTGAATGGCATCACACCTATGTGCATATAGACAGTACTTACTGGACTCAGTAGGTTATAAAAAATTTAAAAAAAGATGGCATGAGAGTAGAAAAGAGATGTCAGGAGGCTTTCTGGGTATGAGAGCTGAAAGGCAGAAGGGTAGACATGATCAAGATACATTGTATTCATATGTGACATACATTAAAATTTTAAAGAATTAAAAATGTTACTCAAGACAGAAGGAAAATTGTAATTACTAAGGTCTAAGTTTGAAGAGCTTACTTTCAGAAGGGCGGCCTGCTGGAATGGATAGCCATTGTTAGCTCTTATGTCTCCCCTAGTCTGAGGCACATGCTGTGTGACATCAACTCTCTAGATCTCAGCGTTCTTTCACAGAGTTCTGAATTCCGTTCCATGCTCATCTAAAAGCTCATCACAACTTGCCCAAACACATGGGAACAAGTGCAATTCCTAATTCTCCCTTTTAGCCTCTGGAGAAATTTAGAATTTGACTGTTAATCGAAAGTAAAATTGGAGTTTTAAAAAAAAGAGAGAGAGACATAAGGTGAAGAAGCCAATGTCTTAGAACGTTCATTTCTCTAAAAAGCCATTAATAGAAGACTGGCTAGACTCTGTATTTTAGCTACACAAATATGGGGTCATGTAAGAGAACTCAATCATAAACCTATTGGCATCATATCATACTTTATGGTTAAGATGAAAAGATTCCACAATGCTGATACATATAAGACACCTGCTTTAATCGTATTTCTCTGCCCTCAAGGTATTCCAAACCAGAAAGGCCAATGCAACAGGTTATTCCTTGCTGCTCTGGGAGAAACCTAAGGGAGCCAAGAATATCAGTGACTATGTCAGCCTTTCCAGGCATAAGAAATGCCACATTCACAAGAGCTGGCGAATGGCATCAGCTTAGCTATGCATCCACTGATGATGGAAAGATAAAAGTGCAGTGCAGCACATGCGCAATAGCACTCAACTGCAAAGGAAGGTGATAGGAAGTGCAAGAGAACAAGAGGAACTGGAAAATGTTATACTGGTGATATAACCTAGGCCCAGGAAGATAAACTGCATTTCTCTCTCATATCTGGATCCTAGCTTAAAATATTTAGATCTGTGTGTTTAACTTCAAGTATATATGTTCACATAGCCAGAAAGGGCCCAGGAGAGAGGAAAGAAGCAGCCTAATAGAGGGGGAGCAATGGTAGAACACGTGATATTAAAACAGGGGGAAGGGTACTGTGGGTTAAAGGCAGGGCAGGTAAAGGAGGGAGAGGAGAGGAGAGGAGAGGATGGGGGGGTTAGTGAAAAAAGCTGCATCCAAGTTGTTATGGAGACCCACTGTGTGGGTGATTTTAAAAAGTGTGATTCTGGAGGGCTTAGAGAGTTAAGAGCACTTGTTGCTTTTGCAGAGGACCCAGGTTCTACTCCCAGCACCCACATGGCAGCTCACAGCCATCTATAACTCTAGTCCCAAGGGAACCTATGCCCTCTTTTAACCTCCCTGGACATTAGGACTCCACGTGCTACACATGCGAATATGCAAGCAAAACACTAACACATGATAAAATAAATAAATCTAAAATATTAAGTATGTAAGATTTTAAGGAAATAAAAAAGTTGAAACAGATCTGCAGCATGGGTAGATAATGCTCCTCCCAGAAAACATAGCTTATCAAATGAAAAATCTCAATGCCATGCATGGAATATTCAGCCAAAGAAACAGACTGTTGGTTGGACACCATAACTGGATGGTAAGACTCTACATCCAAAGATGCCACAGGTTTTGTTTTCAGAACATGGAGAAACCAAGTTGGAACTGACAGGAGTATTTTCCCCCTCCACTGCTGGCTAGCTTACAAGTACCTGAAGGTGCTTTGCAGGCCTCTGGGGTAGAAAAGAGATCAAAGAACCCTGAATACGATAATACTAACCTGCCAGGCACCATGTACCCACTAGAGCAATAGTGACATGACTGTTACAGGGAGGGAGGTAACCAACTGCTTTCTGATTGGAGTTGAGGTCCACTCCACAAGAGAGTCCATGCTTGGTACTGCAAGCCTGAGAAAAACAAAAACCTTGGTCACTGTCCTGGTTTCCTTTTCTTTTGCTGTGACAAAACACTGACTAAAAGCAACTCAAAGGAGAAAAGTATTTACTTAGATTACAGGTTATAACTCACCATCAAGGGATACTGAGGAAGGAACTCAAGGCAGAACTGAACATGGAGGCAGGAACCAAAGCAGAGGCCATGGAAGAACACGGCTTACTGGCTTGCTCCCCATGGCTCCCTGAGCTTGCTTTTTATAAGACTCAAGGCCACCTGCCCAGAGGTGGCACAGTCCACAGTGTGATGGGAACTCTCAGATCAATCATCAATCAAGACTATGCCCCTCCCCCTACAAACATGCCTACAGGTCAATTTGATGGAGGCATTTCTCAACTGGATGTTCCTTCTCTCAGATGACTCTGGCTTGGTGTTAAAAATAAGACTGAAAGCTAAGCACAACAATTATCCCTAGAGTGTAAACTGACGACATGAACACTTCATGATGTGATAAGGGCAAAGTTTTTTATTGTAGGTGAGAGAGATAACAAGCAGGAGTATCTAGGAGAGTTCAGGGCAGAGAGAGTAGACTGAGCTTGGCCAGCAGACTAGACCTGCTCATAAGAGAAACAGCAGAGCAGAGAGAGAAAGGGGAACAGAGGTAGGGCATGGGATGGCAAAAAGAGGAAGAGGAGGGAAAGAGAGCAGAAGACAGAGAGAGCATATATGAATTAGCAGGGTTATAAGGAGGAATAGCTGGGAGAAGGGGAGCCCATGACTGGGGGAGTTGAGGGTAGGGGAGGAGGTGAGAAGAGCTAGGATGCTAGCATGAACTTCGAAATGTATAACAGGTACTTGTGACAATGAGGGATCCCTGAGACCAGCGTGTGCTGTGGTGTGCTAATAGGCTGCACACATTGTCACTTGTCTGTCTGTCTGCCTTTTGAAATTTGGGGGGAAGGAGTTCCCTTTGAACCTGAGACCTTGGTTCCTAAATGATATGGATCTTGGATAGCTCTGACATAGTCCTAGGAGTCCAAAGACCATGCACATGTATAAAGCTGTGTGCCTAAGGAGGATTATAAACACACCTAGAAAGTATGAACTAGCTAAAGTACTCCATCTGGAGGATGCTGGAGGCTCTTCCCAGTGAAGTGGGGGCTGAGATTTGGTTCTCAGCTGTTTGGCTCGTTGCTGCAGACATGGTCCAGGGGCACCCTGAGCCCCTCAGCAAAGACTGGGAGATTTTGTTGTTCTTGTTCCAGAAATTTAAAGAAATCTTTGTCTACTAACCACCTCTAGGCAAATCAGTGTCCACATACGACAAAGGACACAGAGAGTCAGCTGGGGAAAGTCACTAAAGATACCACATAACTCCAACAGGCACAAGAGTCCTGGGGAGACAGAAGACCCTGATCTTCAAAGACCCCACGTTGTACCATTTAAAATATCTGCTTCTCAGGCTGGGAGATGGTGTACATGCTTTCAGTCCCAGTACTGGGGAGGCAGAGGCAGTGTGAGGCCAGCCTCAAACTGCTCTACAGAGTGAGTTCCAGAGTAAATTCCAGGATAGCCAGGGTTACACTAAGAAGTCTTGTCTTGATTAAAAAAATAAAAACAAAAAAAAAACAAAATAAAAAGTAAACAAACAAATAATCTAGTTTTCAAAAATGACTATAAATCATTGAGAAGTAAGGTCTACAAAAAAAAAATAGAACAATCAATCAAAATGCTATTTGAAAAGGCCACGTAAGATTTTGACTGTATTTTGACAAAGATTTAATTTGGCTATTTTAAAATTATGTTCAAAGAAAGCCATGTCTAAAAAATTAAGAGAAAATGTGTTACTATAAAGAATATTTATTTAAAAAAAGGAAACTATAATGAAGAGTCAGGAAAAATTATAGAGTTGGAAACTATAATAAATAACATTAAGCATAATTTAACAGTGAATGTGAGCAGGCAGAAGAAAGAATCCATGAACTGGAAGATGATGGGCTATCTGCGGGTATGAACTGGAAGATGGGCTATCTGTGGGTATGAACTGGAAGATGGGCTATCTGTGGGTATGAACTGGAAGATGGGCTATCTGTGGGTATCCAGAGGAAAGAAAGAAATCAATCCAGGGCTTTCCGTTTGTTCCAATTGTGTGAAGAGCTTGCGTCATTCCCATGCTCACAACAAGAAGCTGAACGAACAGAAAATCAGCAATTCTATAGAAGAATGCTATAGAACTAAAGTCAGGACAGACAGCCACCCCCTAACCCACGCAGACAGATGAACAGCAAGCCAGTTTACCAGCAGCAGAAACCCTCCCAAGAGGCAGCAACAGTAAGAGTCCCAGATAGTCATTATCATATTTCCTGGGGCTAGGGGAAAAGTTTTGAACTCTTGGATATCCAGTCTGAAAGTGGGAAAGATGTATACTCCCTGCTATTGTATGTTAGAGGTTATTGTTTAGAGTTTACAATTACAAGACTGCCTTGAGTCTCAGATATGGTTTTAGATTATTTTAGGTTATATGCATAACTTTAAAATTATACACAGAGAGAAAAACACATACATACACACACAGAGAGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAATTCTCTTAACCACATATATCTTTTCCTTCCAGCTGAGATGTGTAATTGGGTGTCAATGTCTAGGTGACAAGTGTAACATCAGACTCAAAACTTGGCTCAGGTAACGTGAACCCTCTGAAAAAGGAACTTTCTCATCAGAGAAATAATCTGTTCCTGAGAAGAAATCTGCATCTGAACAAAGCCTCAGAGGTAAGTATCCTAGTAGGGGGTATGGGACTTTCTCTGAGGTCACCTTCGATTAAAATTAGTAAGTAAAAAGTAAGTAAAGAAAGAAATTAATAAATGGAGCTTAAAAAAATAAGACAAGAAAAAAAAATCATACAATACAAATTTGCCTAAAATAGGTTTCCTCTCTGACTTTGTAGCTGTTCAACCATGAAGGGTGAGCCTCTACCGGTGTCACTGAATGTGTGCTTGAATCTTAATAGTCAACAGCTTCATGTTGTTTCACTGTTCAGTTGAAACACTGCCAATCACGAATGGCTTTAAATGTAAGGATGTTTAAAAACCTTGATCCGCCACTGCCTTTTTAAATAATCGAAAGGCAACTAGCTTTTGATTAGCAGACTCTCAAACGGTAGACTTTAAATGATCCCAAATAGTATTTATAGTTCTTGGAACAAGAAATGTGCTTAGACATGGAATTTGGAATTCTGAGGTGGGGCTGTGGGTTGATGTCACACAGGCTGGTTCGGGGCTGTGACATTCTGGCAGCCCTGGCAGCCCTCAGACCTCCAACAGAGAGGTACATCAGCCTGCATCTTAAGTGACCTAACAGGTATGTCAGCTTAGATCCTGTATCTTAAGTGACCTAACAGGTATGTCAGCTTAGATCCTTCTTTCTCAAGTCACAGGTTTGAGAAACTCAAAGTTTCTGAAACACCCTATTCCAAAACCGGCTTTTTTGTTGTTTTATTTTGAGGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTAAATAATTATCCTTAATGCTGGACAGATGACTTTGATAAGTATGTTTGTAGTCAGATTGTTTCCTGTCCTTTCTGGGCTTGCTGGTCCCCAGCTTCAGATGTTAGACTAAGAGAAAGATGATGCTGTGGGAGGATTAATAGGGAGAAACTCAGCCTGCTAGAAGACAGGCTGCACCCTAAGAGACTCCTGAGAAAGCTGGGTCGTGGTTTGTGCTAGCCTTTGCCAAGGGGTTTGGCTGGAAGCATTCAGAATCTTTTAAATCCTGACACAGGATTTGTCTGTCTGTTTTCTGGGTTCCCCTCACCCCTCATTAAAAGCACTACTTTCAGAATAGATTTGTACATTCTAAGCAGTTTTGCTCAATAACATTTTGAAGACCATAACGGTCGAACCCAATGTACTTGAGTCACTGTATGCACACTTCAGAAGGCTTGTGTGGGGGTGAGGGCTGGGGCTGGGGGTCCCAGTGCAGGCTTGGTTGTTCTACAAGGGCCTCTCGCTGCCTAATAAATAGATTTTACATTCAAGTCCCTTCTTAACCACAAGTAAACCCTCTTACTCACTTGAGGTCAGCGTGGGCCCGCCTGCAGGGTTCCGTAAGTCATCTTCACTGAAGAAAGTTGGGGAGGGAGGTGGCTAGGTGGCTCTTTGGGACTGAAGCCACTCCAGCTGTCTGGACTTCTTTGGCAGGTGTGAAAGATGTTCGACTTGAGGACGAAGGTTATGATCGGCATCGCTAGCACTCTGCTGATTGCTGCAATCATGCTGATAACGCTTGTGTTCTGTCTTTACCAGAAAATATCCAAGGCCCTGAAGTGAGTCTTGGGAGTCAGAGGGGACAACACTGGGGCCCTCTGCTGGAACAGGGTCACTTCTTATTGCATTGGCCACTTGTCGGGGGAATTCAAGAGCCACTGTCAGGTTTGAGAGTGAAGGCAGGTGGGCACACAAGTTTGAGGGTTCTCCTCCTAGCTTGGGGCTGTGAGCTCCTAAGTTGACTGCTCTCAACCTGGACAGCATGGGTGCCAGCATCACTTGGCTGTCATTTAGACACGGAGTGAACCTTCAGCTACTTCTCAGGGCCCTGGCTGGGCATCCTTTCTACCTCAGCACTGTGGACTGGAGAAGCCAGAGGATGCCCTGGGGATGAGCTGTCTCCTATTCACACAGAGCTTGGCCATAGTGAGCTCTTCTCCACTCACTGGGGAGCTCTCATGTTCCCACAGTGCCAGTGACTTTGGGGAAAGTGACGTGGGGCAAGAGAATAAAGTCCATGGAGGACAGCTGACAGGGATGTAGGTCAGACAAGCGATGGTTTGAAGTTTGGGAGAGAAGTTTTAAGTATGAGAAAAACTGGCTGTCATGATGTCCCTTAAGAGGGACTTAGATTGTAGGGGCCAGAGCTGAGCCCAGCCAACTGCTGTGCCTTCTCTATCATCCCCACACCTCCTGGGCCTGCAGGAGCCCCTGGGGCACAGTTCACTCTCTTTACTTGTGATGGAATCTGGGTTTCACATGCATAGCAATTGTAGGAGAAGAAAAGATGGGAAAGGAGCAGAGAGCTTTAACCCAGGGCTGGTGTCACATGTATGATGGGCTCCAACCCTGGCCTTTCTCACAAAGCCCTCATAATTGTAAGAAGCTCACTGAGAAGGGAAATTCTAAGCTTGTTTTGTGTCTTTTCTTAAGACTCGCAAAGGAGCCTGAATGCTGTATTGATCCATGCAAGGACCCCAATGAGAAGATCATCCGGGCCAAGCCCATTATTGCTGAGACTTGTCGTAACCTCCCGTGTTGTGACGACTGTAGCATCTATAAGGATGTTGGCTCCCTGCCACCTTGCTATTGTGTCACAAATGAGGGACTCTGACATGGCAATGGTGGACACAAAAATCTTCACAAACAGTATTTCCTTCTTAGTAGAATGTTTTATTTTTCAAGTCAATTTCTGTAATGTTTATACTCTTATTTACTATCTAATATGGTATTTATGTACTTTATATAAGCACATAATAATCAAATTACTCCTGTAACTTTAATTGGTGATGGTAATGGTTATGAATGTCCTTTATTCTAAATTTTTTACTCATTCTGAGATTTGAGGCAACTAGCCTGTAATGCTATGTTGCAATGTAAGCAAAGAGGGCATATAGGCAGTTTTAAGGATGGGATATCATTTTGCTAAATTTCTAAAATAATTTAAAAAGTTTTTTAAGGTTAGGTTTAAGACTGAAACTTCCTGTGCCCAAAGAGAAAGCGCATGCCTCTAAGTGAGATGACGTTTTCCATGCTAAGTTCTGCCTGGATTTGTTCCACGCTGCTCCTGTTTTCTTCCTCCCTTTTTCTTTTTCTTTTCTTTTTACTGTACTGTACTTAAAGCTTTACTTTAACTGAAGACGGATTTTTTTTTTTTCATACAATCCATTCCAATTTTAGTTCCATTTCCTCCAACTCCTCACGGATCCTCCCCACCTCCCCACCCACCCAAGTCCACATCCTTTCTTGCTCTCCTACATTAGAAAACAAACGGGCACCTAAAGAAAAAAGAAAAAGTGATAAAATGATAAAGAAACTTGAATAAGACAGATGAAAAGAAAGACAGAGACACAGAGTATGCACAAGCAGCACATACAGACAGACACACACTGGCTACATGGAAATCCCCGACTTATTTCAGAGCAGGAAGCATAATGTATAAGCAAAGTCCTGCAGTGTAAGAAAAAGAAGAAAGAAACCTCGGACAACGCATTGTGAGAGCAAAAAAAAAAAAAAAATGCCCTCCAAAAGCACCACTGAGCTCATTTTGTGCTGGCCGTCTACTTTGGGGCCCAGGGCCCGGCCTGAAGAATGGCTTGTATACACAGTGAGACCCCATTAGAGAATACTAATTTTTTATTTGCAAGTCGTTATCAACTGGAGAGAGCTGCTCAGTCAGGAATGTGGGCTTGTGTCCATTTGCACTTAAATGCTGAAACCCCAAGCCTACGTAGGCCCTTTGTCTGCCACCATCCCTGTGAGTTTGTATGTGTGCCGACATAGAAGGTACTGTTTACTCTGGCTTTTACAGCATTCCCACCCCCTTTTCTGTAGGCTTCCCTAAGTCACATGTGAGGGGAGGGAATTGACTGAGACATCCTACTTAGAATCAAGAATTTCAAGGTTTCTTCATCTCTGAGAAGTGCTCAGAGGCAGGAAGAGGGTTGGTGGCTCTCTTAGAGCTGGAGTTACAGTTGGGAGCTACCTGTCACGGGTGCTAGAAACCAAACTTCAGCCATCTAGAAGTGTAGTAAGATCTCCTAACTAGTGAGCCATGTTTTAGCCCTCAAAAAAATATTTTTATCAGCAATATGAGAATTTTTATGGATGTATCATAATTTACTTAGCCCAAACTTTATTACTGAAAACAGGTATTTTCTAAGTTCTATTTATTAAATATTAAAAAGAGTACCATTTAAAAAATTCTTACCAGTAAGTATATAGTAACAGGAAATAAAACACTTGAAGGTTTGGTGAGTCTAAATTCCCAGGATCAGTTTGACAAATCTGTTACCACGTTGCCAAATTCAAGTCCAAAATGGTTACTTTTCTCAGTGCCGAGACCAATTCCTGATAGTAAAACAGGAAGGAAGTTTGTTCTGCCTTCACAGGTTTAGGGTACAGTGCTCTGGGATAATCCGGTGGCAGGAGCAGGAGACAGCTGGCAACACAGTGCCCTTAGTCAGGAAGCGAGGGAGAGGAAGGCTGCTGCCCAGCTTGCTTTCTCCTCCCCCAAAGGTTCTCTTCTAAATGATTCTAGACCCTGCTGACAACCAGTACTAACCATCACAGGGTGTTAAGAATGAAGGGGAGGCCCACTGCTCTGGGATGCACAGGCCCTAACAGGCCAGACTAAACAAGGATAGAGTTGCTGTATTAGCTAAAGGAATAGAACTTATAGAATGAATACAGTCCAACTAGTCTAACAATGGCTGTCTACCAACGGAAGCTCCAAGAATCCAGTAATTGCTCAGTCCATGAGGCTGGATGTCTCAGCTGGTCTTCAGTACACACCAGAATCCCAAAGAAGAAGCTCTAATGCCATTGAAGGAATGGACTTGCCGGCAAGATTGAGAGCAGGCAGGCTATGAGGAAGTATCCTTTATATATGCTGCTACCAGATAGTGTGCCCAGATTAAAAGTAGGTCTTCCCACTTCAAATGATTAAAGAAGAAATTCCTCTTAGGTGTAGCCAGCTGCTTGGGTGTTAGTCAACTTCAGGTGTAGTCAAGTCGACAACCAAGAACAGACATCTTAAGTCCCCTCCTTATCAACTTGACACACAATTATATCTTCTTATGTCATCCTTAAATCCCAAATGAGAATAACCAGGCCATAATTATACCTTACCTGATTTAACTACCCCATGTACAATCACAAACGCCTTATATATTTAGCCACTTCATAATTACACCTAACATGATATAACTATCCCTCATGCAACTGCAAATACGTAAGCTTGAGAATAGGTGGCAAGGTCCATTGAGGGATATTTTTAGTCTCTCAAACTTAAATATTTTAACCTGATATCCTCTTAATTGATATTACATACACATTGAGGAAAGAAAAATAATTGTACAAACACATTCTTAACAAAGTATAGATCCTGGCTATGGGAGAAACTGTACCTTACACTGAACACTGATTTAAAGCATACACTGCTTTCTAGAGAACCCAGCCCCAGTGTCCCAGGCTGCGGCCACCTAATTAGCACTGGAACTGTGTCTTCAAAAGGCCATTCTATCTTTCAGTCAGGGCAGCTGCTTCAGGATGGTGGAGAGCATCATAATGCCAATTATAAGCCACTTGTGCAACTGATCATGTGGCCTTAGCTGGTATTTTTAAGTATCTTCCTCTATTACCCATTCTGTATTTCCTCCACCTTCAAGCAAGCACCTCAGCAGGTCTTGGCTCTTTTCTCAGAATGACCCGTTACCTTCATTGCTGAAGGGTCCGTGCCCTTTGTCATCCTACCTGGATTAGACTGTTGTAGTTTCCCATTAGCTTTAACCATAACACATGGTAGTAACCAAGAGAGACCCTCAAGACCTCTCGTGGCTCCAAACATCGTTTTTATCTCAATTGTGAAGAAGAATCCAATGCCCCCTTGGTAATCTGGATCAGTCACCCCTCCTAACACTGTCATTTCTTTCCTAGCCTTGGCTTAAGGGCATCAGAAGCCCAGGGGGAAGTCTGAGCTTCCAGTTTAATGGAATGTTTATTAGTGGCTCCTGGTAGGAATGCTTCCAGATCTGGTCTGGGACCAAAACTTCCAGGCCAGCAAAACTTAAGGTCACAGAAAAAGGGGGCAAAATTTTCCCTAATGGTCACTAGGGGTGACAGTGAATAGAACTACTGCCTTTTCCACCTCTTGATTCCTGGAACTGTAGGTCCTGGCTATGGGAGAAACTGTACCTTACACTGAACACTGATTTAAAGCATACACTGCTTTCTAGAGAACCCAGCCCCAGTGTCCCAGGCTTCGGCCACCTAATTAGCACTGGAACTGTGTCTTCAAAAGGCCATTCTATCTTTGTCAGGGCAGCTGCTTCAGGATGGTGGAGAGCATTATAAGACCAGAGGATTCCATGATTGTGGGCCCACTGTCGTACTTCTCTGGCTATGAAGCGAGTTCCTTGGTCAGAAGCAATACTGTGCAGGAAAGGCAATTCCACAAACAGAATAAGTATCTACCCCAGGAAGGAGTACTAGTCCAGTGAAATCAACCTGGCATCAGATAGCTGCTTAGTTATCTCAGGGAATGGTGCCGTATCTGGGGTTCAGTGTTGGTTTCTGCTCTTGACAGATCTGGCACTCAGCATCAGCAGCCATTGCCAACGTTAGCCTCAGTGAGTGGAAATTTATGTTGTTGAGCCCATACATAACCTCCATCTCTGCCACTGTGGTCACTCTGTTCATGTGCCCACTGGGCAATTACAGAGATAGCTGGGTCGAAAGAGGCTGACTGTCCTCAGAACAGGTCATCTTATTTCATTACTGAACTTCTCTGCCGAAGTCGAAGTCACTTTTTTAATGAACATTTACATGGGACACAAGTGATCATCGTACCCTTCATCCATTTGGATCAATCTATCAATAGACTTCTTCCCCAGATGTCTGACAAATTCCCAGTCGTGTTCTTTCCAAGTTCCTGACCATCATATAAAATACATTTATATATATATATAACAAATTTATATAAAATAAAAATATAAAAAATATATAAATATATAAGTATATACTACATTATGTTATGTTTTATATATATATATTGACTTATTAGAATAGCTTACAGGCTGTGGTCCAGCTAGTCCGACAATGCTGTCTACCAATGGAAGGCCCAAGAATCCAGTAGTTGTTCAGTTCACAAGGCTAGATGATTCAGCTGGTCTTCAGTATATACAAGAATCCCAAAGAAGTAGGCTCTACTGCCAGTGACGGAATGGACCTGCCATTGAGAGTGAGAACAAGCAGGCAGAAGAGCAAGTTTCCCCTTCTATGTCCTTCATATAGGCTGCCACCAGAAGGGGTGCCCCAGATTAAAGGTGGATCCTCTTACCTCAAAAGATTTGAATTAAATGTGTATCTTCCCACCTGGAAAGATTCAAATTAAAAGTGGGTTTTCCCTTTTTAAAGGATTTAATTAAGAAGAAGAAGAAAAAAACAAAAACAACAAAAACAACCTTCACAGGTATACCCACATTGTTTGGGTTTTAGCTTATTCCAGATGGGTCAAAGTTCACAAAAATAAGTGAGTTCTGTTTTAGTTCCATATGAACTAGCTTTGAGATAGCCAATCTAGTTTTTATTCTTTAACTGTGCTTCGACGACGACAATGGTGGTAGAGGAAAAGCTCAGCTGTTTTTGACTAAGGCCACTTGACATGTCAGGGTGCACAGCCCCACGATCAAGCATGGTGGCCACAAGAGTTTTTCTTTTTCACACAAAGCCCAATTTGACACTTATGGATCCCATATCAAGCTGTTACCTGAATCTGGAGACTCTGGTACTAGCCACTTCTTTAGGAAAAAAAAAATCGAGAAAAAGGAAATGGGTGTGTTTACAGTTTTAGTCAGAACTGGGGAGGGTTGGGATAGGGAGGTGTTCCTTTCTCCCCACTTTTCCTATAGCAGTGATACAATCTACAGAGAGGATGAAACAAAAGCCAGGAAACAAGTATGTATGTGTATAGACGTAACGGTGAAAGAGCTCCTCAGACCGTGAGCCGTCTTTCCTGGCAGGAGGCTGTCCTAGTTGTGGGCTTCAGGCTATTCCCAACCTAACACAGGACTTCCTGGAGGGTTATAATTCTTCAGGGTCCATGTTACTCTCTGACAGACACTGTATGGTTTATGAAATGTGTTCCCATCTTTTATACTTCTGGCTTTTCTCTAAGTTCTTCCCATTGGCTACTCCTGCTACCTGCTCAGTCCCTAGGTCACAAGGATCAAGTGAGAACCCAGGGACTCTCGGCCGTGGAGGTGAAGCAGGAAAGGTGTGTCAGGCGGACATGAAGACAGTTTCAAGGCCTTCACTTAGGGGTTCAGTCCCATCCTGCAGACCCGGGGAGCCCCAGCTGATGATTGATGGGCCAGAGGGCACTACTAAGTCAAGGGTGGTACTCTGCTAGTTAGGGAGGAGCCACAACTACCACGGGCTGGCTCTCTTCTTACTCATCAAGATATGACATCTTACATCTACACGTGGTGTGACCATCCATCCCAACCTTGGCCCACCAAAGATGGCCAAAGTGTGGTGTGTCCTACATGCTGGGTCATCTTGGCTGTATGATTAAAATCAATAGTTTGTCAAAATACTGGATACGTACAAAATATCCCCCTTAAAAAAAGAAGAAACAACGTAATCAAATTAGCTGAAAAGA
D3_15 138454490 138469538 15049 GAGAATGAGGGGATGGATCAGTGGTTAGAGCACCTGTCATGCACATTGAGGAGCAGAGTTTGGATCCTGTTCAGAACCTACATGGAAGGATAAGTGTGGTGCTCCACATGTCATCTCAGCACTCAGAAGGTGGAGACAGGGAAAACCCCGAAGCAAGCTGGCTAGCCACAACAGCCTTCTGGCAAGTTCTACATTCAACTGAGAAACAGCCTCAATGAATAAGATGTAGAGTGAGACTCCCAGCATCAACCTCAGGCCTCCACAGATGCACACATCCATGCACATAAATCCTCACATGTGCCAATACACCAGTGCACATGTGTACATGCACATACCACACACACATATATACAAATAAGAAAAAAATAATTAAAAGATAAGTCAGAGAGTCTCTGAGGAAGACAACTGATGTCGGCCTCTAGCTTGACATGTGCATGTGTGCACATTCACCCGCTCACGCATGACACATACACACATCAGGAAAAGGGAAAGGAAAAAGAAGTCTGCTGGGAAATACACGAGCACATACCCGCTGCTGATTAAGTCGTAGGTTGGAGATGCAAAGTCAGACGTAGGCGAGGCTCTGCAAGTATCCAGAAACACCACCAGGCTTGGATCCGAGGTGTGCAAGGTGGCTTGGACAAAGAGAGTCTGGTTCAAGTCTACATAATATGGTGACTCCTGTACGAGGTTTTCAAATGAGTCGGACTCATAGAGAGCCAGGCTGGTGTTGTATTTGCCCAGGACACTCTGGTTCTGTATGATGTCGTCTTCAGTTATGTACATAATCTCCACGGTAGAGTTATACTCCATCTCACAGGTCACCACGATCTGGAGGAGCTTCTGTCGGGTGATCACAGCAGAGACCGGAGACTCGATGAAGGTGATCCTGTTGGTATAGCTGATCGCGTGGTCCTCTATCTGCAGAATGCGAAAGAATTCACATTCTTATATTTTAACCCATCACTATGGCAGAGTACAGAGAAAGATAAGACTTGTTGGTAGCAACTTAATTTCCTTTAGTCATGAAATAACAGATTCCACGTCAAAGACTCTCAACCTAACATGTATTTTACGGCAGGGCCTGACCTGCAGAAGGAAGAGAGTGAAAGATACAGCTTGTATTAGAAAGACTGAGAGATTGAAGTGGGTAGAATTTTCTTTCAGTTATATTAACATCATTGTGGACTGCCACTTTCTAGCTCTTTTAAGACTTTAAACTCATCCTATCAGGCCTGTTGGCACAGGCTCCTAGGCAGGCTGAGGCAGAAGGATCACATTCCAAGCCTACATGAGCTACTGCTAGGTCGCCATGGCCTTAATAAGGACAGAATGTCATGGTTCCTGCCCCTGGAGCAGGGCCAGCAGGCATGGGCCTCCACAAACGCCAATGGCTGCTGTGGGTATAAGGCTTGTTTGTATAATAATGTACGTATTTTACGTTCCTCCTTCCAGGAATGTCCTCCTTGTTAATAACTCCATGATAAACAGAGATAGCACAAGTCACGGAGGTGAAGGTGTGGCTAATGTCTCGGCAAAATGGTAAACACTGGAACCTTCAGGAACAGCCCTTAAGACTATAGAAAAGAACTCTAAACACATGAGTTCGAATATATATAATTTCTCAAAAAAATAAGGATGCAATATGAATGATATGAGGGGTTTCACAAATCTAAAGGAACAAAAGCTTTTGTGAGCCAACTTGTCAGAAAGATACAAAGTAGATAAAGAGATTTAGGGAGTGGTAATTGCAGGAGCTCCCACCCAGCTACGTTTTTCAGACAGATTCCTGAGTTCAGGGTAAGCCTTGGACAAAGCAGGGTTAGACCCAGGTGTGGAGAAATGGTAATTTCACAGTGGGGTTCTACCTAGCTAGTTTATCATCTATGTTTAAGAGAGGTAGGCGGATCTCTGAATTGCTTTGCAATGTTAAAGGAGATGTGTTCTTGCTCCTCCTAAGAATTAAGGGACTGGAGTCATAAGATGCTGCTTCATAGGATAATCGAAATGGAACCTGGAGTAAATGACTGGATTGATATGTAAAATGAAAGACTGAGTTTATGATCTGCAAGAGATGAGCTATTCATAGATTTTTTTTAGAGAGAGACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCTCGCTCCTTGGAGAAGGAGCAGGAGCAGAACATAGAGAAACCAATATGGAAAGCTGTCTCCAGCAGAACATAAGTCATCAGCTTGGACCCATGATTTGACTTCGAGTCCTTTATTTTGCTTCTCCCAGACACCCCTTGCAGAACCCCTCTCCAGGACAAGGCTGGTCCTTGGCAAGCTACAGAGTTCAAGGTCAGCTTGGACAACTCAGTGAGATCCTGTTTTGAAACATAAGGTAGGAAGAGGGCTGAGAATACAGCTCCGAGGCAGAGGGCTTGCTTGCCCAGCGTGTGTGAGGCCCTGGAGTTGATAAATAGAAGACTGAACGCTGCAACACGTTCCCTCTGTAAGGAGGCCTAAGTTGGTGTGTCTAGCCAGGCAGATCCCCAAATGTGCACAGATGGAAGCTAGGCCTTGGTACACCATAGACGTCAGTGAAATAATAAGACCTGTGTGTTCAGTTTTGGAAGAAATAAATAAACAAAAGAAAATATTTACGTTTTATCAACTCCTCTGGTCTAGCATCTATCCTCAGTCACAAAGTGATTTAAGTTTATGGTATGTATATTAAGAGTGTTGACAGTGCTTAAGATCTTAAATGGTATTTCTAAATCATGCAGACCAAATCGCAGACTTAAAACTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCCTCTTCCTCCTCTTCCTCTTCTTCTTCTTCCTCCTCTTCCTCCTCTTCCTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCTCCTCCTCCTCCTCCTCCTCTTCCTCCTCTTCCTCCTCTTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTTTTTTTTTTTGGTGTTTTGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGAAACTCTCACTCTGTAGACCAGGCTAGCCTCGAACTCAGAAATTCACCTGCCTTTGCCTCCCAAGTGCTGGGGTTAAAGGCGTGCGCCACCACACCCGGCCTGACTTATAACTCTTAATACCTAAAATATGATTTTACTGTCTGCCTTTTCAAGGATAATAAGATCTTAAACCCAACTCAATTTTACCAAATACAGAAAGGTGATATTCTGCACAACTTTTCCCAGTAGGCTAAGAAACCCTGACCACCAAAGATGTTCCAAGTCTCTCTAGTGAGGCTTCAGAGCTCTCTGCCTTCTGTGAAAAGCCAGCCATCCTCCAGTTCCCCTGCAAATGAATAAGCAAGCATGCCTTCGTGTGTGAGTGAATGTGTACACAGCTATGAGCATGCCTTCGTGTGTGAGTGAACATGTACACAGCTATGTTCATGCCTTCCCAGTCCAACCATGTGTGTCTGCTGTGGTTTGTGAAAGATACAGCAAGTTTCATACCTGTGCATCACCACGTGGCACATCTGCCAGCATTTGACAGAACCCAGCTGTGGGTTTCACAGTACCTTTTTGACTGTACCGCATTCGTGCAGAGGGATAGAGAACTCTATGACATTTGATACATTCGGTCTGCAAGTTGGGTCATTTAGCTGCAAATTGCTCTCATTGTAGTTGAGTGCCGGTAGGTATGATTTGCTTATGATGACTCTCATCTTGTCAGAAACACAACTTAGAGATGCTATCAAGAAAAGGGGGAAAGAATGATCACATTTGGGCCAGCTAGGAACTTTGCATTTTTAAAACTTACATGTCCAGACTATTCCATAAAAAGTTTAACCTGCTGGGACTGGTGCCAGACACCCTTACTTCTAGCAGCTAGGAGGGAAAGGTATGTGGATCTGTGTGAATTCTAGGTCTACATAGTGAGTTCCAGGATAGCCCGGGCTACATAGTGTCAAAAAGAAAAGGACAATAACAGCAACAAACAGCCTGAACCCTCTGCGCTGATAATATGGCTGTCAGCAAGAGAACTTTCTGCCCAAGCATGATAGCCTGACTTAGAGCCCCCAGCGCCCCCACTAAACCCAAGTATGGCTGTGTATGTTCCTGTAACCCAAGCGCTGTTGGGAACTGAGACAGGAGGATCACTAGGGTTGGCTGGCTGCTAGCCAACCTCCAGATTAAGACCCTGTCTCAGGGGAACAAGGCAGAAAGTGCAAGAGCAGGACACCCAATGTCCTCCTCTGGCCTCTACACACACACCACCACCACCACCACCACCACCACACACACACACACACACATACCACGAATGCATGACCACTCTCACACACAATAAATAAATAAATAAATAAATAAATAAATAAATAAATATCTGAACTCACAATACATTAAATAGTAGGTGGAAGATTGGCACAGCCTAGTAGTTTGTGAGCTTTTTACAGTGCCCACTGCTACCTTGTTTAGAACTATAATCTATACATCTGATTTACTTGTATAATCTATAGATCTGATTACAGCTACATAACTTTCCCCCTCTTTTCCTTTCACCCCTAAGCGCCTCCCAAGTGCCCCCTTCTAATTCTTTTCATGCCCCCAATTCTAAATTCATAGCCTCTTCTTACCCAATTATTATTGTTACATTTCTTTAAAACTATGTCATTACAGAAGACATTAAGCCAACCAGATAGAGTCATTCACTTCCTCCTGTAGTAAGCAAGGCACAGGGTGACTCCTCAAGCCTCCAGGTTAGCTATATTCCCAAGTACATGACAGGTTGGCAAACAAGGCTAGCCACAATCAGGCTAAGAATCAAGGAGACGGCTCTACGTAAGTAATCATCAGAGTTCAAAATAGCTGAGGGTCGTGAAAAGACTTACTAGTGTTGACATCGTGGATGTAAATTGAAGTGTAGGAAGCAGAAAAGCCTTTGTAGGAATTGGCATAATCTGTAGATAGCACGACAGTCATGGCGTCAGAGGAAGATTCTAAGGTAGGCTTCCCACGGCCGCAGAGTTGTTTGAGCAGGCCTGCCGTGGTGGAGGGGCCATCGTAGACTGCGATGAAATCAAACTTGCAGTTTTCATCCATCTCTAGTCTAAGAAAGACAGCCTCTGGTGTAAGAGCTTTTCAAACAGATTCAGTCCTGTTTTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTTTGTTTTTTTATCATTCCTTCTAAAGTCATGAAGGATTTTTCCCCTTACAATGACATCTCAGATTTATGATGGCAGACCTGGAACTTTGTCAGTCCCTCAGAACAATAGTCAACAGCACTGGTATGTGTGTTTCTTTCTACAGTGCTTCTCTGAAAAGTACCTACATGTGGGAGCATGTGATTTTTTTTTCTCCCCAGGTTGGTCCCCCAGTGGCTTGTGTGTCACTGCAGTAGACCACAATACTTACAAGAGATCTGTAAACTTCAATTGTATCTTATAGCCTTTCCCCACTTGTATGTGCCAGACACAATATGCCAGCTCAGGGTGCGGCTTTGGATAATTGGGGCTGCTGAAGGACCCTTCCAATGCTCGCAGGTCACCACCGCAGTTTGGAATAACTAGAAAGATAATACAAAGATGATTGGATGTTTCTGTGATGTATGGAGTTTAACTTGTCCTCCCTGAGTGTCACACCATCCCTGTCTCCTCTGTGAGTCACATTGGAGGTTTTCTTCTTCTTCTCTTAGCATCAGACCTTTTTGCCTTGGGGTACCTGAGAGCATAGTGCTGCCCTGCTGTCAAATCATGCAAAGTGACTGAACTGGCGACAGAGGCCCTCCCACCTGGACTGAGCTCCTGAAAGTAGTCACACTCTTGGGGCGAGTGCTGGAGGCTCCTCACCCACCGTTGCTGTCCAGGCTCACTGGCTATCACCAAAGGAGCTGTTGTTCTAAAGTGCCCTTTCTTAACATGTCTGCTAGGGCTTCAGTATAGCGTCCTCTGCTATTCTCTCCTCGGGCTAGTATTCAAAGTTCAGCTCAGATGCAACCTTCCTCCATCCTTCCCTGACATGATAGGTACTGTAATGGGCAACATGACAAATAAACACAGAAGCAAACGGAAGACCCCTAGGTTGCTATAAAATAGTTTAGGTTGGTGGACTTGTAGGACCTATGTTAAGTAGGGTCTCCACGTCAGGTGTGAGGAGGTAGCCTGTGTCCAGCTCCAGAAGAGCTTGATGCCTCTACCTTGTTGGCTCTGTAGCACAAAGATATCAGTGTCAAGATCAGATCTTCTAGAAAACCAGAGTGAAGCTCTCCTTATGAACCTGCTGGCTCAAGCCCATGGCGTTCTACCTAAGGTAAAACTGGAATTGGGGGCCAATGTCATAGAATTGTGCAGCACCCGACTGCCCTAAAACTGGCTCAGCCTCACCCACAATTGCTTGGTTGTCGGAAGAACTTGATGGATACACAAAAGCTCAAGAGCCTCAAACCACGTCTAATGACTGTCGCGGAGGTCTCAAGGTTATCAAACAGGCTGAACAGAGCCCTCTGGGCGCAGACACAGTGCCTGCTAACCTGAAGCTGTGTGGTGAGCCAGGGGAACTGCATCAGGAGCTTCCCCAGACCCAAAGAATCAGATTTCAAACATGGCGACTGAACACGTGACTCTTCTGTGCATGGCACAGAGAAGACTGTGAGGGAACAGATTCAAGAGCACCGTGTCCCCAGCAGGACCTGGTGTAACCTGCGCTTGCGAAGGGCAGGGGAAAGAATGTGTCATGGACAACTTACCGGTAGCAGCGGAGAAAAAATAGTAGAAGATAAAGACACTCCTTGGGAACTTTGTCAAGCCAGTGACTATCTGAAATGTCATCGAGTTGGATGATGATTCAAACACAGGCACGAAATCATTTCTGCTGCAGGCCTTCCCTAGCAGAGGCCCACTGGTGGAGCTTCCATCAAACACCTTAATGTTTTCAGTTTCACACCTGCTGCCTGGATCCAACCTGGGAACAGAACACACCTGTAGCTGGAGAAGAGCCACTAGCAAAGTGGCTTTTCCAAGGCATTGTTCCTGGTTTGCATTAGCGCAAGAGTTTATTAAGTGAGGGCAATCTGGCTCATTTAAAACAGTCATGAGCTCCATCCCTCTTCAAAATCAATCAAACCACAATCTTAATGGGCTCAGGAAAGTGAACTAACTTGCAGCACTTTATACTTAGTAAATGAACAAAAACGGGCCTAGAATTCGGGGCTGCATTCCTCCTCCCCCTCCCCCTCCCCCTTCCATCGCCCCTGTTCCCTGAGACCGCATGGTGACTGTTTAGTATAGATCTTTCTAATTTAGAATCCAGCCTCACTGATATTTGATATCTAAGCTTGCTGAAAAAACAACTCCTCCTCCATGGCCACCTCCCGGTGAAGCATCCCCCAGCTGCCTCTTCCTCCCCACCAAAGGCTCATTCTGGAGGGTGGTCCAATGCTCTGCACTGAACAGGGAGCCCAGGATTTCTATTTTCTGTGCACGCCGTCAGGCTCTTTCCAAACACCCATAGAGGATCTATGTCTAAGAATAGAATCTTAACTCCCTTCCACCTCAAACGCTTCCCTGATGTATATATAGCTTTGGACCAGGAACTTACCATGCTTGGCCTGTTCTCCTCTTCTGTACAAGGTTTTTTATTTTGCCATCTTAGCATGATGAACCTTCCATGGGCTTCATACACAAGAAGTTGCCCCAATAAATGACTTCTTTCTATTGAGTTTTGCTCTAACCACAATCCTGCTCTCTCTCTTTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCATATATATATATATGGAGATACATATATAGGGAGAGAGAGATAAAAAAAATGGGGGAGGGGGTATAGGTTTCCAGTGTTCCAGAAAGTCAATGAGTTGCTGTCTTCGCATTTCACTGAAGGTCAGTCAGTTCCAGCTTCTGTGACGTAGAGCCCTCTGGTTCTCCAAGTATGGTGGCAGAAACAGCAGGATCAGAGTGACTTGAGAATGTCTCTTATGCAGGCTTTTCAGGCTCCACCTATCTTATTTAAATTAGATAGTATAGACCCCACCCCAGCCCAGCATGTGCACTTTGATGAGCCCTCTTGGTGAATCTGACAGCTATGGAGCAGAGCAAGAACATCTACTTATATAATAGGAGTCCTGGAGCAAGAATGCTGGGATTCCATTCCCTTGGACTAGTGGCCTTAGTCCAGAAAGTGGCTTGTCTGAGCTCTCGTATCTACTGAATTAGGTTGGACCTTTTGCAGAGGGGTGTTGAGAAATCAGTTGGGGCAGATCTTATGAAGTCCCAAGGCCTTGTTGAACAGAGTCTCAGGTCTTAGCATGGTACCATCAAGGCATACAGTACATGTATGCTGCTTTTAAAATAGCATTTCAAAAACTATCAAGGTTAGGTCACATCCTCTGAGACTCTGATCTAACTGTTCAGGGTGCACCCTAGGAAACTTCCATTATGGCTCTAACAGAGACCAGAATTTGGAGCCAGAGTTTAAGATTTTAAGACCTACAGTCTAGCAGTAAATAGCTGGGTACCAACCTTGATCGATGTGTACACTCTTTGATGAACCTCCACAAGCTAACAAGCTCCTTGTGCCTGTAGCTCTGGCATTGGCCTCGTAGACACCATATGACCCAGATTTTTGCATTCCAGTCTTTGGTGATCACCAGGGGCCATAACCGTTACTGCCATCATCATGGGGATCACGTCAGCCTCCGGAAGCAGCTGAGTCCCCTCTTCAGAGAGCTCATCTCCCACCCCAACATGGCTAGAGTCAGCTCCTTCCTTGAGAAATTGTAGCCTCTACTTACTTGATGTAGGAGAAGATGATTCTGATGCTCCTGTTTTCAGGTCTCTCTATGGTCCAAGTGCAGTTCTCATTCGCACTGAGCTGTAGGACCAGGGCTTTATGGGTCTCTCCCAGATTGGCCCCTCCCAGACTGGCTGTGCATCTTGATTTGCCTGGTAGATACCATAGTTGGCACTAAGGAAAGAGCAGTCCCTGTCTCCCAGCAGGCACTAGGACTCAACCTCCCACAGGAAGGGGAAGTAGTTGCCCAACATATAACAAACTCCACAATTTGTTGTGTGCTTTTATTTGATTATAGTTTTCTCTCCCTTAGGGTGTTGATGTATTTTGCTTTCTAGGTTTTTAGGATTTTGTTGTTGTATTGGGCTTGCGGGTTGTTTTGAGAAAGAACTTAAAGTGGGGTGGATAGGGAGGAAACAAGGATCTGGGAGGACTTGGAGGAAGGGAAGAATATGATCAAAATAGGTTTAAATTTAAAAATTGTTAGCTGGCAGCGGTGGCACACACCTTTGATCCCAGTGGCTGGGAGGCAGAAGCAGGCGGACCTTTGTGAGCACAAGACCAGCCTGCTCTGCAGAGTGAGTTCCAGGACATCCCAGGACTACACATTGAAACTCTGTCTCAAAAAAGTGTTTTATATAACAAAAAAATTATTGGTCAAAAATGATGGGGCCGGTGAGGTGGATCAGTGGGTCAAGGCCCTCGTTGCCAAAGCTGACAACTCATATCCAGCTCACAGAACCTGCAAGGTAGGAAAGAGCCAACTTCTGGAAGTTATCCTCTGACCTTCACTTGAGTATTCTAAGATGTGAGTACATGCGTACTTGAGCACACACAATAAGTAAATGTAAAAAATAAGTTAAAGACCAGAAACCAAAGAAGAAATGAGGAAAGGAAGATAGGGAGGCATACATTTGTAACTGCAGCGTTCAGAAGGCTGAAGGAGGAGGCTCCCATGCAAAGAGGCCAGTCTGAGATTTGTAGACAGATCCTAACTCCCTCTGCCTCTCCCTCTCCCTCTCTTCCTCCCACCCTCCGTGTGTGTCTGTCTGTCTGCCTCTCTCATTGTCCAGAAAGGACAAGAATAGACACATTTTCCAAAAAAAGACTGATAAAACCCCTACGTTACTTAGAAAGAAACCTCGGTATGTTCTCTTTCACCCACGGCTAATGAATGAACGATCTATCTATCTTTACCTAAGAATACGAGGTCTACATTTGTCAGGAAAAAGAAAGCAAAGATAACAGAAGAGATGGCACAGTCCCACCCACCCAGGGGCAGCATCTTTGTTAAAACCTTCTGCAAATTCATCTGTCTTTTCCTTCCCTCCTCTCTTTTTGATTTAAAAGATGTTCTGTGCATACTTCTAGACATAAATGGTGTCATATTGTAAAACTTCAGGCATTTTCCCTCCTAACAGAGACCATTTCTTGTAGATGTAATATTTTAGAACTTAACAGATTATTTCTCTGTTATTGGTTTGCCCTTTAAAAAAAAAACAGCACCTGAAACTTACATTTTTTTTATAAGAATCAGGCCAATATCTATTCGTGTAGGAATCATTTTCCTAAGATATGCACTAAAAGTTAAATTGTGTAGCCCAGGCTGGCCCCGAACACGTGATCCTCCTGCCCTCTGCCTCCTTCAGCCAATCCTACCGGTGTGTGACACCACAACCGGCACATTTGAAATGTAAATAAAGAAAATATCTAATAAAAAAAGTGAAATTGTGTGTGCCTTTTTTTTTATTTCAATGACCATGGCCATATGGGCCTTAATGAGATGACTATGAAGCCCAGAGAACACAAGGGTGAGTTCTCTGTATTGAGGGCCGTGGCAGATACAAAAGCTTTCAAAGTTTCGGGAGAAGCCTGGTAAAAGCCAGTCTTCTTTGTTTGAGTTGAAGAATCTGGTTCAATTTTCTTACCTTCAGCCTCTGTAGAATTCAGCTGTGCCCCACAGGAGACAGCTAAAATAGCCCAGATGAACAGCCTTCCAGTGACCTCCATCTTCGCAGGGTGCCTCAGGGCCCTCACCCTCAGGCTTCTTATAATTCTCCGAGTTTTGTAAGCTTCCTGGTATCTAGGGAAACGTGTCAGTTTGGCCATCCCAGGGGTGGTGCTGGTGAGAACTCCACAGCTGAGGAGCTGCAACCTGGCCAGCAGTTCCTGGCACTGGGCTCAGTCTTCCAGGTCACATCTCAGAGGGCTATAAGCCAGCCAGCCCGATTCTGCACAGGGCTCAGCAGCTACGTAGCAGTTTCTCTGGCCTTGAAACTGAATTTGAGCCTTGACCCTGTCTGTGCAGGTCATGAATGAAGGAGCAATGCACCAGGATCTGAAGCTCAATGAGCCAGGGCATAGCAAACTCTTGGACCCAGCTGGGCCCTCAGATGCCTACAGGAGGCTGACCCAAGGGGTAGGGGAGGGACAAAGGATGTGGAAGAAAAGACAATGTAGATCAGATCTCCCTGCAACCCTGCAAGGATTCCTGACCCCTGTCCCTCTGAAGAGGCTAACATGGATGGATTAGAATCCCTCAGGCACTGAGCTTGTACCTCAGAGATAGAAGTCCTAGTGTCCAACATGAGACTAGGGAGAAGAGTGGGCATGTGAAAGGGACTGTCCATTCACTCATTCATTCATTCATTCACTTATTTATTCATTCACTGAGCAAGTTTTCATTGCTCACTCTCCTAGGATGTGGGAATACAAAGCTGAGTACAAGATACGGAGACTGTACTCTTATGGAACCTTTAGTCCAAGTGGGGAAAACATAATGAATTAATCACAGTTCATACAGGGGCAAGTGTGTTGAGTGTGCTACTGGGAGATCGGATAGAATGTGGCAGCCTTGGGTGGGAAAGAATGCAAATTAGGGCAAATGAAAGGCATCTCGAGAAGATGAAATGGAGGCAAGAACAAAAAGAGGGAGACAATCATGTATCTTACTTGAGGAATTTTGTTTAAGATAAAAGTATGTGTATAAAGGCCCTGGGTGCAAGAACTTCTACGCACTGACTACGTGTGAAGGAGAGGGAAGTGGGGAGGCAGGCAGAGGAAAGGTCAGGGGACAGTTGATGGAGACAGACCTGTGTGGCAGAGAAAAGCTCAGTCTTGTTCTAATCTCACGTGGAACTTTTCAGTGTATGGCCAGCTCTAGGCATAGTGGCTACAAATGAGAACGGGCCCCATAGGCTCATGTAATTGAATTCTTGGTCCCCAGATGGTAGAATTGTATAGGAAGGATTAGGAGGTGTGGCCTTGTTGGAGGAAGTGTGACACTGAGGGTGGGCTTTCTGCCTCATGCTTGTGGATCAGATGTAAGCTCTCAGCTTCTGCTTCATTGCCCTGCCTGCCTACAGCCATGCTCCCCACCATAATGGTCACTGACTCACCCCCTGAAACTGTAAGCCCCAATCTTCTATAAGTTGCCTTGGTCATGGTGTCATATCCTAGCAACAGAAAAGTAACTAAGACACTAGGAATCACACAGGAGCTCCTTGAAGATCAAGGTGAGGCAAGGTCACCTAGAATGGAGCCTTCTTTCCCTCTCTTTAGAAGGCACCCCTTTAGCTCCTTGGGTACTTTCTCTAGCTCCTTCTTTAGGGGCCCTGTGTTCCATTCAACACGTGACTGTGAGCATCCACTTCTGTATTTGCCAGGCACTGGCATAGCCTCACAAGAGATAGCTATATCAGGGTCCTGTCAGCAAAATCTTGCTGGCATATGCAATAGTGTCTGGGTTTGGTGGTTGTATATGGGATGGATCCCCAGGTGGGGCAGTCTCTGGATGGTCCTTTCTTCTATCTCAGCACCGAACTTTGTCTCTGTAACTCCTTCCATGGGTATTTTGTTCCCCATTCTAAGAAGGAACAAAGTATCTACACTTTGTCTTCCTTCTTCTTGAGTTTCATGTGTTTTGCAAATTGTATCTTGGGTAGTATAAGTTTCTGGGCTAATATCCACTTATCAGTGAGTGCATATCATGTGTGTTCTTTTTTGTGATTGGGTTACCTCACTCAGGATGATATCCTCCAGATCCATCCATTTGCCTAAGCATTTCATAAATTCATTGTTTTTAATTGCTGAGTAGTACTCCATTGTGTAAATGTGCCACATTTTCTGTATCCATTCCTCTGTTGAGGGGCATCTGGGTTCTTTCCAGCTTCTGGCTATTATAAATAAGGCTGCTATGAACATAGTGGAACATGTATCCTTATTACAAGTTGGAACATCTTCTGGGTATATGCCCAGGAGAGGTATGGCTGGATCTTCTGGTAGTACTGTGTCCAATTTTCTGATTTCCAGAGTTGTTTTGCTTTTTTATACAATATTTGTCCTCAAATACAACTTTGGTAAATAAATAAATAAATAAATAAATAAATAAGCAACATAGTCTGAGATAGAGATCGAGGGCATTACTAGAGAAGGAAATGGCTTTGATGTCCATAGCAATAAACTGTTCTGTCCGTGGCCATTTAAGCAGACAACCCAGGCATAACAGGACAAGGGGTTCTGGAGGTGGAGGCTGACTTAATTGTTCTGAGAAATCTTGCCAACTCATAATGATTAAGTTGTACCAGGAAACATTTGAAGCAGGAAATGTTTTTGAGAAGCCTGAAGTTTTGGTAGTTAAGAAAACTGAAGATCCCCAAGGGAAGGGAACAGTGGGCCTGGGTCACCAAGGACTGGGATGTTCATTAAAAAATACAAGGGACAAGAAAGAGGAAGACATAGCTGGGTCCACTGGATTAGTGAGCAATGGTGAGAGCCCCCACGAGTGGAGATCTCTAATAATAGAAAGTTTGATCCAGAAATATAATTGGCGAGGACAGGAAGATATATGTATAATAAAAAAAGATAAAAGGTTGGGTAGGAGAGTTTGGAGCCCATGAGGGGCCATTGGATTAGTGGACTGACTTTCTGGGGAGACCGGGACCTACTGATCTGATCCCCTGGCATTGTCTGCCTAAGGGAACTAGGGTCCAAGTTCTAAGAGACAGAAGTCAGGCTGGGAAGGATCTGTGGGGGAAGCTGCAAGAGGAATTAACCTTGCATATGGAAAGGTTTGAAAAGCACTGCACAGGTAGACATTTGGATGACTTAATAAAGAGATTCTGCCGATGTAGACAATTAAAGGCTAACATTACCCTGACCTGTTTCCCATATTTCTGTTCTCAGGCTGATACTGTAACCGCACATTGACGACTTGTGGTTTGAAGACTTTTCTGAGAGCTAAAAGTTAATATGTAACATGCTTTACCATACGCTGAACTGTGACTTTGTATGTGCATTGTGCTTTCTGGTGACACAGTCGCATGGGGTTTTTTTTGGGGGGGGGGGAATAACAGGCTTGAGGAAAATAATGAAAAGGCTTTTGATTATGTATTATTATATAACAAGGACATGTGCAACCAATAAAAGACAGAGGTGGACCCAGAGAGGAAAAAAAAGGACAAACCTGAGAGAGAGAAAAATCTGAAGGAGGGAGGGAGAAAGGGAGGAGGAGGGGAGAAGGGAGGGGAAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGGGAGAGCGAGAGGGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGAGAGAGCTGTCTCTGAGCTCAGCTTCAGAACAGCAATGGTGACCTGTCAGCTTGCACCTGCATTTGTGCCTGAGTCTCTGATTTTGTGCCAATCCAATCCTCCCATTCTTCAGAACTCCAAAATGACTGAGGTTGGTCCTCGGTGGTAATACATATAGTGGTATAAGAAATATAACTAGGAAACATAATAGAGGCAGAGATTGTTTTCACTATTGTACATTTATACAAAAGTACATAAACACAATGTTAGATAGTATACAACATAAACACTATAGAAATTGGC
GF3 -1 -1 1 GACAGAACACAAGCGTTATCAGCATGATTG
GF4 -1 -1 1 GTGGTTAAGAGAATTCTCTCTCTCTCTC
EX52 -1 -1 1 CCTACCAGGCCAACACCATTGCTGTTGC
EX32 -1 -1 1 CACTGGAGGGGACCGCAACTCGCAACAGC
GR3 -1 -1 1 GTCCAAGTGCAGTTCTCATTCGCACTGAG
GR4 -1 -1 1 GAGTATTCTAAGATGTGAGTACATGCGTAC
Comments:
Created
Target: 2803Ensembl ENSMUSG00000030858 -- 138469590 -- 138470841 ENSMUSE00000204404 -- ENSMUSE00000204403
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: 7, start 138469590: end 138470841
Found 4 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
REJECT (1) gap pair G5:7:138476341:138477340_324 G3:7:138465090:138466089_511
REJECT (3) gap pair G5:7:138476341:138477340_324 G3:7:138465090:138466089_356
REJECT (3) gap pair G5:7:138476341:138477340_324 G3:7:138465090:138466089_363
REJECT (1) gap pair G5:7:138476341:138477340_805 G3:7:138465090:138466089_511
ACCEPT (0) gap pair G5:7:138476341:138477340_805 G3:7:138465090:138466089_356
ACCEPT (0) gap pair G5:7:138476341:138477340_805 G3:7:138465090:138466089_363
ACCEPT (0) gap pair G5:7:138476341:138477340_208 G3:7:138465090:138466089_511
REJECT (1) gap pair G5:7:138476341:138477340_208 G3:7:138465090:138466089_356
REJECT (1) gap pair G5:7:138476341:138477340_208 G3:7:138465090:138466089_363
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

webmaster@sanger.ac.uk

Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK  Tel:+44 (0)1223 834244