High Throughput Gene Targeting: Create Design

This custom vector design tool is provided by the EUCOMM consortium as a service to the research community. When using this tool, the user agrees not to submit more than five designs per day for creation without prior discussion with the High Throughput Gene Targeting Group at the Sanger Institute.

Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by cs10
Valid After Annotation
yes
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 91660
Chr: 15
Strand: 1
Status: Ordered
BACs: RP24-459L3 : RP24-90N1 : RP24-121F15 : RP24-333A17 : RP24-185H23 : RP24-76F1 : RP24-460N24 : RP24-356E1 : RP24-449B14 : RP24-172L21 :
Instance: Plate 111: Well D11: BACs RP24-90N1 RP24-121F15 RP24-459L3 RP24-333A17
Oligos on GRCm38:
Type Start End Length Seq
G5 82341573 82341622 50 TAATTGTATTTGCTTACGGAGTGTCTCTTTTGCTGGAAAGTAGATTTCAA
U5 82347480 82347529 50 TATTGGAGCTGGAGTTACTTGCGTGTGAGAGGCCACGTGGGTGCTGAGAA
U3 82347546 82347595 50 TGCTGTCAGTGCTCCTCACCACTGGGCCATCCCTCCAGCCCCGGAACTCA
D5 82348143 82348192 50 GCAGTGGGTTCACCACTCGAGACTTACTCTCTCTCATGTTGGATGACTTA
D3 82348206 82348255 50 TTAAGGTCCTCAGTGGCCTTCTCATTGTCCTTGATGGACTTCAGTCAACT
G3 82352281 82352330 50 GGTGTTAAACATTCTGGCTACAGAGGTCAGTATATTCATTTAGTGAAGAT
Oligos:
Type Start End Length Seq
D5_D3 82178623 82178635 13 CACTCCCACTAGG
G5 82172003 82172052 50 TAATTGTATTTGCTTACGGAGTGTCTCTTTTGCTGGAAAGTAGATTTCAA
U5 82177910 82177959 50 TATTGGAGCTGGAGTTACTTGCGTGTGAGAGGCCACGTGGGTGCTGAGAA
U3 82177976 82178025 50 TGCTGTCAGTGCTCCTCACCACTGGGCCATCCCTCCAGCCCCGGAACTCA
D5 82178573 82178622 50 GCAGTGGGTTCACCACTCGAGACTTACTCTCTCTCATGTTGGATGACTTA
D3 82178636 82178685 50 TTAAGGTCCTCAGTGGCCTTCTCATTGTCCTTGATGGACTTCAGTCAACT
G3 82182711 82182760 50 GGTGTTAAACATTCTGGCTACAGAGGTCAGTATATTCATTTAGTGAAGAT
G5_U5 82172003 82177959 5957 TAATTGTATTTGCTTACGGAGTGTCTCTTTTGCTGGAAAGTAGATTTCAAGAGGACGGGGACCTAGTTTTGTCTACTGATGCATTCCTACAAATTCAGCAAGTCTGCACAGGGCTGATTATTGGCGATTTAACCACTCTAGATGTCCTGCAAATAGGGAGGGGAGGTGCGCGAAAGGGACAAAGCCAAATTCGATCTTTGGGTACATCAGTAATCAGGAGACTTCTCCCCAATATCGTGGTGACGGAAATCCCATAGGGACAGCCAGGAAATCTTAACTGCACTTAACTGACCTACCTAGCCCCAGGGAGCCCAGAATTACAGTCAAGAGACAGTGGGCCAAGACATACAGGGAACTTCAGTTGCCCTACGGGATGAGCAGATTATGAAAAGGAGGAGCGCTTCCGGCAAAAGGCTGCCCTTTTACTGGGGCCCTGAAGTTAGAACAGTTCTCACGTTCTCTGAGAACCCTTTTGCAGGCTCTCTGGAAACCATGTGGCTGAGCTGTGTGGTTAGATAAGAGGCCACCCTGTCCCCTTCTGTGACTCTGCTGATAAGTGTGTCACCAACAAGGCCCCACCTAGAAGTTATAAGACCAAGTGCTGACTCTTCCTCATATGACCCTTGCCAGTTCAGCTGTATAGGAACCTTGGAGCACCAGAATTTAGTGGCTATGTCTTCAGTGCCCCAGGACACACCGCTGGAGTTTACAGAGCCGGTCCCATTCACCCCTGGTCACCTCCTCCTTCCTCTGGTGCTCGTTGGTGTGATGTGGTTACCCCTAGGTGGTCTCGGTCACTGGCCTGTCCCTCCCCTGCCTCCATCTGGCTCTGAAATGTGGGCTGGATACCACTGGGGCTAGTAGGGGCTCAGGAAAGTCGTGGGCTCCCTGGAGGTTGAGCTTACACTTGCAGTGAGGGGCCCCATGTTTAGGATGTTTAAAGCACAAAAGTGTCTTTGGGCCTAGGGTCCCTTTCATCCCTCCCCAGGGGTTATTTCTTTGGGTTAAAAAAAAATGTCAAAAGGAATATCCAGGTTAGCCAGGGATATGGTATGACCCAGCCTGAAAGCCATGATTAAAAACTACAGCCATACCACCCTGAACACGACTGATCTTGTTTGATCTTGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTATTCGGATGGGAGACCAACTGGGAATACTGGGTGCTGTATGCTTAAAACAAACAAACAAACAAACAAACGAACACCCAGAAGAGCTGGGGCTCCGTGTGCTCAGGGCTCCTGCCTTCAAAACCTAGCATGACAGAAGCTGAGCATGGGGTAGGTATGCAGGAAGAACTGGGCAGATGGGGACAGTCAGCTCAGAAGCTGAATTCAGATCAGACATCCTTGGCTACATAGTGAGTTCAAGAGCAGCCTGGGCTATGAGACTCTGTCTTCAAACAAACAAAGCACCATGTTGGGCTCTTCTCCTGATACAACTATTGTCTTTTTTGTTACAGTGTTTTGATGTCCAGGTTCTTTTTTTTTTTTTTTTTAAATTTTTTTGGTGACCCTTATAAGACTTTTGAGATGAAAGGCAAGGAAGACTAGTCCTTCAGCTGAAGTCTGAGAACTGCAGGAACATGGTCTGTAGGAGCCACAGGACTGGGCTGCTGGTCTTCTGAGCTGGGTCCTTCCCTGCGGGTGCTACCATCTCTCTATCTCACTGTTCTCGTGGGAGCCATGAAGCTGAACAAGAGGAGTGTGGCCCACTATGCGCTGAGTGATTCTCCGGCAGACCACACTGGCTTTCTGCGTAGTTGGGGAGGACCAGGTTCCCCACCCACTCCCAGTGGCACTGGCCGAAGATACTGGTTTGTTCTCAAGGGCAATCTGCTATTCTCTTTCGAGACTAGAGAGAGCCGGGTCCCGCTGAGTCTTGTGGTATTGGAAGGCTGCACGGTGGAGCTGGCCGAGGCTCCTGTACCCGAGGAGTTCGCCTTCGCTATTCGCTTTGATGCCCCTGGAGTGCGCCCACACCTGCTGGCAGCAGATGGGCAGGCTGCCCAAGAGGCATGGGTGAAGGCACTGTCCCGAGCCAGCTTTGGCTACATGCGACTTGTGGTGCGAGAATTGGAGAGCCAGTTGCAGGATGCCCGTCAGAGCCTGGCCTTGCATCGCTGTGCATCCCAGAGGGCTGTTGCCAATTGTAGTAAATCTCAGGCTCCTGACCACCGAGCTCCAGACCCTGAGAACGGCCACTTTCTCCCCAGGGACCGCAGTTCCATTGGTACTGTGGAAGAAAGGGGGATCCGGCCAATAGGTCGGGATTTGACCGAGTGGGAGTTACAGGGCCCTGCTAGCCTCCTCCTAAGCATGGGACAGAGCCCCGTGTCCCCTGAGAGCTCCTGTTTCTCTACCCTGCATGACTGGTATGGGAAGGAGATCATGGAGCTGAGGCGAGGGTGGCAGCAAAGGGCAAAAGGAAGCCAGACAGAGAACAAGTCACAGAATAGGCCCTGAGCCAGAGCCCTTTAAGCTATGGCCCTGTCCTGCTGGTCAGGATGCCGGGGTCAGAACGTCAGGGGTTAAATATTTACTCATTTACCAGGTTGCTCTGTGTGTGTGTGTGTGTGTTCCCCTGCTCTGTAGACTTTCTCTAGCTTCCAGATCCTCCCCTCTCGCTGGCATGAACTGAAGAATCATGGCTCCCTACTTATTTTCTGAGGTCTAGAAAGGCCAGGTGGTTGATGGGAAGATAATATAATTCAGGAAGTGGGGCTCTGCTCTCTAGCCATAAAGTCCAACACAAGAAGTCTTGGGCATCTCCATGCCAGAGGTGACAGGAAGTGGCTTGAAGACTGACATCGTTGTAGCTTGGGCAGGGCTCTCTGTAGGAGAAGGTCTTTAGGTGCTAAAGAGGGATTTGTTCTCTCTCCCGCTGCTGCCCTGTCCATGTATAGTGCTGGACATTGCCCGCAGGGAGGCAGGCAGCCTTTTATATAGAAGACCTGACCTGTCGTCATGGCAACTTGTAGGGACTCTTGTTACCTAGCCCGCTTGTTCCTAAGGCTTGTCTGCTGAGGGCTCTCATAGCTACTCTGTCCCGTGTCATTCCAAGTATCTTTTTCTTTAGCTCTAAGGTCAGATGGCTAGGTCTACAGAGCCAGAAACCAGGCCCGTCTGTCCCCTTTATATAGATGTAGTGACAGTGGCTGTGGCTCAATAGAGATGTATACCGTTCCCAAACAAAATTGGACTCTTCCTGACCTCCCTCCTTTCCCTATCTGGGGCCTTGGGCCAGCCTGACCCAAATCCAACAAGGCCTAAAGGCTGGATTTTGCCTCCAGGAGACTGAGTTCCATGGTTCCTCTGGTCAGGGCTATGTTTCTCAAGCCTGCTTGTAACCTCATAGCCAGCTGCCCTCACTGTACACACAGGTACCTTTGCTAAGTTTCTTGTCACAGTAGTTAGTGTTTGCTCTTTTAATAGTTTTGGTTTTTGGAGACCAAGTCTTTGTAGCCCACGCAGGTCTGGAACTCCTTAGCATGGCAATCTGCTGGGATTATAGATAGGAGTGCCACACCTCACTTGGTTTTGGTGTTTGTTTTGCAGCAGGACCTGGTGGAAGGCCTGTTAGCCTCCCCTCCCCCCTTCCCTTTATAACCAGAACGGTAAAGTTCCCCGGTTGGTAGCAAAGAGGTGGAGGAGGATCAGGAGTTCAAAAGCATCCTTGACAACACCGCGAGTTTTTTGGCTAGATTGGGCTATGTGTGAGACCCTCTCAGAAAGTTAATTTTGGTTATTACAATCAACGTGTTTACAAATGAAGAATCAGGAGCCTGAGTTGCAAAGCTGGTGTCTGAGGCTAACAATTGAGTCCAAGAACCTGGCTTGGTGGTATGACCTCCATCAGGCCAATCCGTCTATTTGTTTGTTCCCTAGATCTGTAGGGGAAGCCCGCTGTCCACTGCACCCATTGTACCAATGTCCACAGAAACCATGAGCTATTTATTGTTAACTTGATTTTTTTCGCCGCACTACGTAGCTCCGGCTGTCCTGGAACTTACTGGATTTGAACTCACAGAGATCCTCCTGCCTCTGCATCCCAAGTAGTGTAAGATTAAAGGTGTGCGCCACTTCGCCCAGCTTTTTGTTAACTTTTTGTTACCTTAAAAAAAAAAAAAAAATATATATATATATATATATATATATATATATAAATTCAGATACCAAGTAGAGTTTTTCATTACACATTCTGGTTCCTTAATTCAAGTCTTCCCGAGGTGAGGGGCGGGAACACCTACAAAGGGAGCCCTGGCGGCCCCGCCCTCTGGAGTCCGGGAGGGGCGTCCTTCTCCTGACGTCACTACGTCGTCCGGGCGCCGAGGACTTTCCCACCGGCATACGAATGCGTGCTCTGCGCAGGCGGGAGGGCGGTGCTTCCCGTGTGTGTCACGAGTCTCGCGAGTTTTGGAGAAAGTTTCTGCCAGAGGCCGCCTTGGATGCGTGGGGTGTGGCTCCCGGCGAGGGGCGGAGCTGGAGATGGCGTCCACGGCGGCTCGGCGGCTGGCCTGGGTTGCAGTTCGACCCGGGGCTCTCTGGAGCGGGCCGAGGGGGAGGAGAGGTGGCGATGTCTACACCGTACCGGGCAGCTCAGGTCTCAGCCAGGTACCGTCGAGGTCAGTCATCGTCACTCGCAGCGGCGCCATTTTGCCCAAGCCGGTGAAAGTGAGTGTCTGTCTTGAGGCGGGACGAAGGGATTGAGGCCGGTGATTGGGGGAGTGCATGTGACCTGTCCTGATTGACAGGCGCAGAGGCCAATCATAGTATTGGCATAGACTGGGGACCGGACATAGGACTCAATTTGTGCAGCTGGGTTTTGCTGACTTTGACAGGTGGAGGAAAATGTTTCCATAAAACTCACTGTTAAGCTGGGTTCCTGGACACAGAAGCTGGAAGGCAGCAGCGAAATGTGCAATTTCTTATCTTGAGGGACATAATGACCTATTTCTTCAGGGTCAGAAGAAAGGCTTTGTGTCGATTTATACAAGTCATGCAATCTCTGCCTCAAAGGTGATGGGATAAAACAATCCTGAGAGCTGGAGAGATGGCTCAGAGCTTAGGAACTCTGCTCTTCCAGAGGACCCAGGTTTAACTCCCATCACCACAGGACAGCTACAACTATCAGTAACTTCAAGATCTGACATCTTCGCATAGTCATACACGAGGAGGCAAAATACCAATGCACATAGAATAAATTACTGAAAGAGTGGGAAGGGAATGCGGAAGTAATATACTTAGTAAAAAGGAAATGAAGGAGAACGGATAGGGGGATGTGGGAGCAGGACCTTCTGTGCTGAGGAATGTGTAGGTTTTACAGATGAAGTCAGGGCAGAGCCCTGAAGCCAGCATCCAAAGCTAGGAGTTAGGTCTTGACAACCAGAGACCACATCCTGGTTTTTCCATACTTGAACTCTTGTCACTTGCCCTCGGTACCCCAAAGGAAAAATGGGAAGTCTCTTATACCAAAGGTGTGCTTTTTGACGGCATGGCCGTATGGAGTCGAAGGGAAAACAAAAGTCTTTAGAGCAAGCCTGAGCGGTAGCAGTTGACCCCAGTCAGGGTTCCAGCGAGAAGCAAAAATGTGCAGCTGCTGTATAAAAAGTCACAGCTAGGGGAAGCAGGAAGCTAATACCGGTGAAATCTCATTGGCATTCCTTCCGTCCATGTTGCCTTTGCGAGCTTTTAACATTAATTGTGAAGCAAGGTTGCTCAGCAGCAGGAGCCAGCTAAAAGAGAAGGAGGTGAGAGGCCCCATCAGTGCACACACCTCCTCCTAACTCGAGTTTTATTTATGTGTATGCATGTTTTGCCTGCATGTATGTCTGTGTAGCACTTAAGTGCCACATGCCCACAAATGCTAGAAGAGACTGTGCCATATTGGAGCTGGAGTTACTTGCGTGTGAGAGGCCACGTGGGTGCTGAGAA
U3_D5 82177976 82178622 647 TGCTGTCAGTGCTCCTCACCACTGGGCCATCCCTCCAGCCCCGGAACTCACTTTTGGGTGGAGAGAGCTGGTTAGAGCCCAGCCAGCCTAGTGTTCTCACCTAACTTTGTATGGTTCTTGCAAGGAATTAGGTTTGGCCCCTAATTTTGAGTTGCTTAGTTTCCTAAATTTAGTTTAGCTTTATGTTTTTCTTTCTGATATCCATTTTGGTGGCCATAAAACTCACTTTGTCTCTTCGTTGCTCTGAGTTTCTTCAAAGACTTTAAAAATCGAGTGGTGTGGGGATCAGTAGTCCATTGGAGGCTTGGGCGCAGCCACTGGTCTCCCGGAGTGCACCCATTCTTCTCTCTGCAGATGTCCTTTGGTCTTCTCCGAGTGTTCTCCATTGTGATCCCCTTTCTCTATGTCGGGACACTCATCAGCAAGAACTTCGCTGCTCTGCTTGAGGAACATGACATTTTTGTCCCAGAGGATGACGACGACGACGATTAACAGGTAATCCTTGCCCTTCTTGTAAATGGGCAGAAGCAGCAGGCGGATGATCTGCTCTGTGGTGCTCTGGCCTGCGGCACCATCTGAACCCTCAGGGCAGGAGCAGCAGTGGGTTCACCACTCGAGACTTACTCTCTCTCATGTTGGATGACTTA
D3_G3 82178636 82182760 4125 TTAAGGTCCTCAGTGGCCTTCTCATTGTCCTTGATGGACTTCAGTCAACTGACATTCTCTTAATGTGCTTTTTATTTCATGAAGTCATGCAATCTCTGCCTCAGAGATGGAGGGATAAAACAGCAAATGATGGAGGAGAGGAAAGGGAATGCAGAAGATACCAGTAAGGAGATGGGCAAAGGGTGATCCAGGAAGGCATACCACAGGAACTCTCACTTCCTACTTTACATTGTTAAAGCTGCTTTGTATGGTTTCTCCTCCTCCCCCGCTGTTTCTTGAGTTACCCACATCCTTACCCATCTCCAAGTATTGGCCCATCCTCCTTCCACCGTGTAATCCTGTCATCTTCCTTTACCCTATTTGGAAGTGACTGCTGCTAAAGCCTGGTGTGGGATCTTGAGCTTTTGTGATCCCAGCACTGCAGAAGTAGAGGCACAGCTCAGGGGTTAACAGGAGAACTTATCTCAGAAAACAGAAATGCCCCAGGCCTGGTGGCACACAGCAGTAATCCCAGCACCGGGGAGGTGGAGGCAGGAACACCAGAACTTCAAGATCCTCCTTGGCTACAGTGGGCTTGAGACTACTCTGGGCTGTGCGAGGCTGTCTTACAAGTGACTGATGCACGCCTTTCTTACAGGGCACAGGGAGCACAGGAGAAAGCACTGATGATGTGGTGGCATGCTCAGCTCCCTCCCGTCGGCAGAAGGGTCAAGGAAAGCCCTCAGCCTCACCTCCTCATGGTTTGTGACAAGAGCCCAGAGTCCTGTGCTTTGGGAGGCTCCCTCACGGCTGTCCACAGAGTACTGGAAATAACAAGCAAGCAAATTAGGAGTGTATAATAAATGAGTCATGTATGATGAGAGGTCGTCCCCATTTCTCACTCCCAGCAGGCTTGGTTGCACTTTATTGTCTCTTCCGTGTCCAAGAAGCACCTGGTGTAGAGCCAGGACATCCAGGCTATTGTACAACCACAGAGGCAATTGAGGAGGACTGAGATCTTTCCTTTAAAATGATTAACTATGTATCTGTTTTCAGGTGCTCTAGGAGGCAGCATTCCCCCTAAAGCTGGAGTTAAAGGTTATTATGAATCCCTTGATGTGGGTGCTGGGAACTAACTCTGGTCCTCTGCAAGAGCAGCAAGTGCCCTTAACCACTGAGCCATCTTTCCACACACACCCTCCTCTGACTTGAAATTTTTCTACTTTCCATAAGTTCAGGAGAAACTTGCTTCAAAGCCACTTAGGCCTGGAAGGGTTGCCATGTGCTGAACATGCAAAGCTTTGTTAACTGCTTAGCAGAATATTTGATAACACTTGGGCCCCTGGGGGGGGGGGCGGGGAGGATCCCTGGTGCTGTGGGGTCATTCAGTAATTAAACCCCAATACTGTGAGTGCCTTTCTTCAGCATTCAGGACTGTCCAGGGAAGAAGGTCGAAACAAGAACATCGGTAGTTCAAGGTCATCCCTGGCTACACAGGGAGCAGGCAGGCTATCCTTGGCTACATGAGATCCTGTTTGAACAAGAAAGTGCATTAGTAGTAGGACCAGTGTGTTGTGTTTGCACTGACTAGTGTGCTCTGCAGTGAGGAGGCAGCTAAGCTCATTTGCAGACCAGTGCTCCTAAGCAGAAGGGCTAGCCTCCGGGCAGTCAGTTCTAGTGCTGTCCCCACAGTGCTGCTCTGTTCCCTCTGATGGAAGAGCCTTGCTGCCAAGTCTTGGAAGCTATATACATAAGTACAGAGACAGTAACAAAGTTACCATTTCACATCCACTGTGGGAGAATCGGATAGAGCCTCCCATCCCTTCCTGCTACCCCATGCAAGGTCTGAGTCAGCTGGCAGCAAACAACATAGATCTCCAGGACAGGGGCATCAACATGGTATGTGTGTACAAGAAGGTTCCACACTCAGGCAAGAAGTAAGAGAGACCACAGGAAAGGGGTTCACCAGTGCAATTTTATCACAACCAACCATCAAATAATGAGTTTATTTATACTCTAGTTAAATATTAACATGCACCTTCCCATCAGGTGACCTCATGGGTCATGGCCCATATAGAACTTAGATAAGAAATCCTTTGGCCTTGGTGTTTCTGTTTCATGGAAAAACCGCATAACGTGTGTCCGCTGCTTCCATACTTTGATGGTTTCCTGGAGCTCCATCTTTCCCTGTGTACATGAAGGAAAAGTAGTCAGTTCCAGCCATTAAATGAGGAACACAGCACACAAGTTCAGTTCTGTGTTGTGTTTGGCATGGACTCGCTAATGACCGTACATTAACCATACCCAGAGCCACACTGCCTTCCTGTGGTTGCAGACAGGTGAGACAGCGTGTGTTCCAGGGTGGCATCCCATAGGAGCTAGCAATTCCTACACTTGACACTGTTATAGCTGCAGCATGTCCCTCCTCCCTCCCCCAGACCTCCCCTTCAGGGCCGAGGCAAGAGGATGTGTGGTTCCAGCCCAGGACTCTATCTCAAAAGTGGAGACATCCAAGTGGGGTATGGTGGTACACACTTTTAATCTTGGCACTTGGCAGGCAGAGGCAGGTGGATCTCAGGGCTAGCCTGGTCTACAGAACAAGTCCCAGGACAGCCAGAACTACATACTGAGACCTTGGCTTTAAAAAGAAAAAAGGGGACCTCCAAGTTGAACTTGGAGACACGGCAGCTGCTGAGTCTAGAGCTGGCTTTAATGGAGGTGGTATTCACTTGAAGACTCAGGGGCTCAGGACAAGCCAGCGGCTCTTTTAAGTCTGCCAGAGAGGGCCAGTTACCTTAATGACCAGCAGATCAACCACTCTGGGGTCTGTGACATGGGCATTCTTCATGAACATTTCTCGGACTTTATCCCGTCCTTGTTTCACCGTGATATCCAGCTGCATTAAGTGCACTAAAGAGAAAACTGGACTGTAGAAATCAGACCTTTTAAGCATGGTTTTAGAAGCTTATGTCAGTGGGTGACCACTGGACATGTACAAGTGGCTCAGTTACTTTATTCCGCATAGCCCAACCCTAGCCTGGATACATGCCTTACTAAACTGCCCAACCTGGATCTCAGTGCTCTTGAGTGGGCTTCTGGAGAGGCTGTGTACCTACCTTGCTTTTTGTGTATGGGCGGAGCAGGCTGAGGCAGAATCTAGGGCCTTGCACATGTTAGGCAAGGGCTCCATCACTGAGGAATATTGTTTTTATATGGGATATAAAATATACTACCATATTATTTTTATATGGAATAACCAGCAAGAATAAAGGCACCAGAGTTGAAATCTCTGTAAAATAATGTGTAGGTGTTAAGTACTATATGAATTTGGGAGCTAAGGGGTACTGCCAAGAGTTTGCACAGGGAGGGAAGGCTGTAATCAAACCATCAGACAACCTGGGGAAGGACATGACTCTAAGCCTTAACTGAGAGTGGGACAGTCACCTATGACTGCAAACAAGATACAAGGCTGCCACTGTGGAGCCATGAGTCACACCACCCTGCACCACCACCCCCAGATAAGGGACTGAAACCGAACACAATAAACTTCTCTTCCCTTAAAAAGGGAGGAGGCTGAGAGGTGGCTCAGCATCGCCTTTCCAATCCCCAGCAACCATGTCAGGCAACTCAAAAACCCTGTAACTCCAGCTCCAGGGACCCACCCATGCCTCTGACCTCTGCGGACACTTGGGATTTACCTGCACCCTCCCATACACACACACACACACACACACACACACACACACACACACACACACACACACACACAACTTTAAAAAGTAATAAAATACATTTAAAAAGTCACAGTATTCACCAATCTATTAAGTCAGTGGCTAAACAGTGGCACAAACTCAGGTGACTGAACTTAATGTGCTACATGGGTGTCCTTTGGAGATTAGGTTGTAAACTGGACTTAGTGGTAACAGGACTGTAAACCCAGCCTACAGGCTGGAGGTTTGCCGTGGCTGTGTATGGTGCTATGAATCCACAGCAAGATCTTGTTTCAAAAGAGAAAAGCAGGCTTTAGTCACCCATCCCCTGACACTCAGCCTTCGGCAGATTTGCTGAGGACACCAAGGATACAAAAGGATCACTAGGTGTTAAACATTCTGGCTACAGAGGTCAGTATATTCATTTAGTGAAGAT
U5_15 82162911 82177959 15049 ATTTGTGCCCGGGATTCATCGAAGCTCAGGCCAAAGTTCCCGATGAGCAGCTGGGGATGGAGGAAAGGAGCAGTCAGTGCTGGCCTCTTCCCACAGGTGGGCACCAGGGCCACTGTAGCCAAGGCAGGCCTGGCTTAGCACAGCCAAGGTTACTGGGCCATATTCCGTTTAGCATCAGCTCCTCCCACCCTCCGTGCAGGTCAGGGTGATGGGCCTTCCTTTTGTGGCTGACTCAGGTGATGAGATGGGATCCTGGGGCCAGAAAGTTTGTGAGTTGAGAGATGCAGGCTAATAGATACAAGGGCCTGCTCTGGACTAGAGGGTGTTCAGGGTTAGCCTGCACAACTCAGTGAGATTTCTCTCAAAGTAGAAAAATATAAAAAGAGTGAAATGTATAGCCTAGTGTAGGGTGCTTGCCTAGGGTGTGTAGAGACTGGGTTCAACCTTAATACAGGGAGAAAAAAAACAACAACAAACAAACAAAACAAACAAAAGCCAAAAAAACCCCAAACCAACCAACCAACCAACCAACCAACCAAACAAACAAACAAAAAACAGAGGAAAGGCTGTCTGGGCTCTGTGACTCCTGCCAGTAATTCCAGAACTTGGGAGGAAGAAGCAAGAAGGTCAGGCATTCAAGGTCAACCTTGGCTACATTAAGTCTCCACTAAACGAGACTGTCTCAAAAACCAACATGGAAAACGCACACCTTTAACCCCAGCACTGGGAGGGTAGAGGCAGGGGGGTCTCCATGAGTTCAAGGGCAGCACAGTCTATAAAGCCAACCAGGGGTATACAGATGTCTCAAACAGAACAACATAAAATAAAACAAAGAAAGAAAGAGAGGGGGAGGAGGGAGAGGGAAAGGGAGGGAGGAAAGGAAGAAATGCTAGAGAGAAGTGGTAGGACCCAGTCTTAGAGAGCTCTCGTAACTGCCGCTGGCCACTCCGTGCCACCGCTGGATGGACCACAGCGATTCCTGGCATTGGCCACCCTTTGCCACAGCCACAGCTAGAAGGACAATAGGGATTCCTGACCGTAAGCAACCAAGCAGTCCACAGTCAAGATGCAAGTAATTATAGCTGAAGCAGTAGCTCCTGTCATCATTCTCGCTTGCCCAATGATGAGACTGAGGCATAGAAGATATTAAGGGGATTATGGGGGGGGGGGGTAGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGTTCACAGCCATCTGTAATGGGATCCGTTGCTGGTGTGTTTGAAGATAGTGGCAGTATATCTTATATACATAAAATAAATCAATCTTAAAAAAAAAAGAAGATATCCAGGCCAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGAAGAGAGGAGAGGAGAGGGGAGGGGAGGGAAGGGGAGGGGAGGGGAGGGGAGGGGAGGGGAGGAGAGGAGAGGAGAGGAGAGGAACACGCCAGGTTTGGATCAGGAGATCTAAAGCAGGTGATTCAGGTCTCCAGACACACACACACACACACACACACACACACACACACACACACACACACCCTAGTTGTCATTCATGGTGATGCTTGCTGACTAAATGACCTGATAATCACAGGAGTATCTGTGTAAGCCTGCTGGGCTGTGATTGAGATCCGAGGACTAAAAGCAGGATGGAGGGCCAGCATGCTGGTGGAGGGGGCGGGACAGGGCTGGGGACAGGCAGGACCTTGGCAGGGTGAGAGCTGAGCATCAGAGGAGCCAGAGTAGGGACGGGAAGGGACAGAGGAAGGGTAAGCTGGATGGACGCTACAGGTGGGCTTTCAGAACTCTTTCGGAGACATCAGACTGTCCTGGGGAAGTGCTTAACAATGGGGAGTCCCAAACTCTGCTCTTGAACATTCTGATGTGAAAGAGGGGGCAGGGCGGAGTTTAGAAGACCCCAACACACTCAGTCCAGGGACACAGTTTAGTGTTGACCTAGGCCCTAAAACAGTGTGGTTCTCAACCCCTGTGAATCCAGACCTTAAAGGGGGGGAGGGGTGTCACAATCAGATATTTGCATTACAATTCATAACAGGAGCAAAATTAGTTATGAAGAAGTAGGAAAAGAATTGTATGGTCGTGGGGTCACCACAGCGTGAGGAACTGTATTACAGGGCTGCAGCATTAGGAAGGCTGAGAAGCACTGCCCTAGGAGAACAAGAAGCACGGGGTGGTACGCTGTAAGGGTTGCAGGGATGGAATGGATGATTGGGCTAGAGACCCCGTCTTCTTCCAGGCTGGTACCATGTCTGGGTCATTCCAGTGCCCAGGACCCGCCACCGGCTGCAGTACATCCTGGTGTCTCACAAACCAGTCCAGGATGGACAGCACACTCTTCCAGGAGTCCTGGATGTCTTTATAGTTGCGCCAGAGATTGCAGACCCTGGAAACCTCGGTGTAGTTCACCTGAACATCAGAAAAGGCAGGAGTTAGCACCAGTCACCTTGCTGCTCAAGGGGTCACCCACCAGCTGGGGAGGCTAACAGTTGGAGACTTGGAATGAGGCAGAGAACAGGTTCTCATGGCGTGTAGTCTGGGAATGTGGCCCCATTTTGGGAATGCCCAGTCCTTCTCTCCATATATATAGGGGAGGGGGCATCCCCATCACACACACGCCCACCACCATGTGCCACATACATACCATACATACCCATTTCTTCAGTGACAGTGCCACCACCTGCCTCATTTAGGTTTGGAGGTCTGGGCCCTTCTGAGGCTATGCATGGTTTCATCTGCCTTCTGGGCCACCCAGGGATTCTGACCACTTGCCTGCCTTGGATGCAACCCCAGCAGCCTGATAAGCTGCCAGAGGCTATGGCTCAGTGACCTGGTCTCTAGACACCTACATATGGCTGTGTTGGCTACTCTAGCTGTGGTGGCAGCCTCCCAGAGGCCTGGCACATAGGAGGTGAGGGGGCATGGGAGTGGGAAGCCGTAGCAGACAGAATGGCTTACCTTGGGGGGTAGGCCCCCTTCATAGGCTGGCCAGCTGCAAGAAAAGGCAATGGGACGGCCCGTGGCGTTCAGGGCAGCAGCCATCTTGGGGTACCCTGCAGAAACCCCAATTTGGTGACTTGACCAGGCACAGGGGACAGCCTAGGACGTGCCTCCCTGTACCTAAAGGCTTACTTCCTGGGTGCCAAGCCTTAGGAGGTAGGTATGGTGACATAGCTACCTTTAGGAGGCCTGGGAGCTGTAAGCAGGGGTGAGGGATTTGGGACAACTGCCACCATTGTATGCAGACACAGCTCCACAGCTGCCGATCTCTCTCTGCGTCCCAGCCAGATGTGGATGGACTCACCCTCTGCACGTTCTCTGGAGCTGGAGAAACAGCCATCCAACTTCAGCATGTCCACCTTCCACTCCGCGAAGGTTTCCGCGTCCAGCTCCACTTTGTCTAGGGTGGTACCAGGGTAACCCATGCAGGTCATTTTGCCCATGTCCTCATAGATGCCCAGCTTCAGACCCAAAGAGTGAGCCTGCGGGGGTTGAAGAGACACGGCTCAAGTGAGACCCTCTTCTCAGGCACCTCTGCTAGAGCCCATTAAACTCTTAAATGGCTTCCAGGACTGGGGATGTAAATTAACTGGCAGAGTGCTTGCCTAACACATGTGAAAAGTCCTGGGCTCTCTTCCCCACACACTGCATAAACTGTGCATGGTGACATGTAGACGTGTTTGTTTGCTTGAGACAGGGCCTCTCTATGTAACCCTGGCTATCCTGGAACTCTCTATGTAGACCAGGCTGGCCTCGAACTCACATAGATCTGCCTGCCTCTGGAGCACAGGATTAAACACATGCATCACCATACCCAGCTAGCACCAGAAGTTTAAGGTTATCCTCAGCTACAGAGTGTTGGAGGTCATCCTGGGCTACACAAGACCCTGTCTCAATAAAGAAGAAGAAACTCTCAGTGGCTCCCACTGACCAAAAGAACCAGTGGCTGGTTTCTGCCAGCTGTGGCCACTTTTTCTCCAGCTGTCTCTTGCCTGGAAGCTTTTGCTACTGCTTCAGAGGTCCCACCTCTGGGATGGATTCAACCAGATGCCTCCTCCAGGGGACTGTTCTTTTTGCTAAGGCCCCTTTCTGGCTCTCGCCTCATTAGGCCAATAATATGTGTGTGTTCCTTTTTCAGTTTCTGCCACTATGCCGCATTCTCCATGGAGACTGTGATAATGAGTCCCAGGCCCTCACACTAAGTAAGGGGCAGGGCTGGAGCTGGACTTACATAGTCAGCTAGGAAGGCAATCCCGTGAGGGAAGCGCTTGGGATCAGGAATTAGGCGGCCTGAGGCGTCACGCCCACCAATCCAGCAGTCATCGATGTTGAGGTATACATAGCCCAGGTCCCGCCATCCATCCTGGGCCAGTCTGTCCGCCATCTCCATAAAGAGCCGTTCACTAGCAAGGGACAGTGGGGCTGTGCTGCTCAGTTCCCCAGGATGTCCCTCATTCCCGCTCTTTCTCTCTGCTCTGAATTCTGGCTATAAAAAACCAAGCCTCTACTGTCAGCTGAGGTGGGGCCAGGATAACAAGTGTAAATGACCTTCCAGGGTTAGGGTAGGGCGAGAAAGAGGTGGTCTGGCATCCAGGCCAAAGGGCAGGACTAGGCCTAGGGAAGCCTGTGGGGCCTGTCTGGCATTTTATCTTCTTTCAGTTACCTCAGAGGCCAAGTACACCTACTGAGGCCCCAAGCAGGTCATGGGGCTAGGTGTCAGATACATTCATTCTGACTCTGCATTTTTCTATTTAAAAAAATTTTTTTAAAATTTTTGCTGATGAGTGTCTTTCACCCACTGACCCACTGACCCCATTTTGTTGCCCTGGGTAAGCAGATCTTACTCACCTGATGCAGTTCTTCGGGTCCTCCACACAGTCGATGTTACAGCGGAAGCGTTCCCAGGCCAGCCAGCCCATGGGTGGAGTCCGCAGTAGCCCATTCTCCAGCATCAGTACCTGAGCCACCAGGGCCAGTAAGAGCACTGAGGGGGAGGTGGATGGTGACTTGTAAATGGTTCCTACAGCACCCCACCCCCCAACCCCAGTCATCACCTCAACCCCTCCAGCCCTTCAGCCCTCCCCAGGTACAGGCCAGTAGGGTTAGAGTTGCCTCAGCCTGTGAGTGCAGTCTGGGGGGGGGGGTACATGGGGCAAAGTAATGTATTCAAAAAGCTGTTGCTACAGACTGGGAATCAATGGAACGGACCCCTGAAGTCATGTCCTTGATATACATGAGACCAGATGCCCTCTTCCCTCCCTCCCTACACAGAAATGACAGTTCATTGTGGACTCTGGGTTTCTGAGGCCTTGGAGTTTTTGCTCTCCACCTCAGAAAGCCTCAGAAGGGCCTAAAGGGGTTGGTCAGGGTGGACTTGTACCCAGCTGCTCACCTACACAAGCCCCTTCCTCTTGGCTTTCAGCTTCCTTATATATAAATTACCACACCCACATGACAGGGCTGCTACAGCCCGCCAGGATCGGGTAAGATAGGACCTATACACTGGAGCAGGGCTGAAAAAGGAAGTGGCCTCTTTGGAGGGGAAACGATTCTGAGGTGTGGGGCATCTCCTTGATTTTCCTGGCCCAGCTCCAAGGAGAGGCACACTTCTCCCCACATACCCCCATCAAGAAAGAGGAGGGCTGGGGCTTCCTCGCCACAGTCCTCCCCTGCAAAGCCACCTGAGTAGGTGAGGGTACCTGTCTTCTGCAACATTGCCTTGGAGTCAAATCTCAGGACCCACTCGAATCTGCTCTTAGCAGCTTTATATGCTGAACTGTGGAAGATAACAGATGTTATGTATCCCACCGGGCATAGTGGGACCCACCTTTAATCTCAGCACTCAGGAGGTAGAAGCAGGAGGATCTCTGAGTTCTGGGCCAGCCTGGTCCACATAGCCAGTTTCAGGCCAGCCAGGGCTACTAGGTAGAGAGACCCTATCTCCGAAAATAAAAGTTACGGATTCCACATTTGGTCCCAAGTCGCTTTCACTACCCTCACACACTCCTTCCCTACCCTTTACTTCCCGGAATCTAAGCTCTTCTTTTAGAACTACAAATAGTCTAGGACACTTTGCCCAAGAGCGGTTGTGACTGGTTCTAGGAATACCAGGAAGAAGGAAGGCTATAGGCCATGTCCGGTTCCAGGTCCCCGTGTGGGTTCAGATTCTCGGTTCTTAAACATCGCAGGCCCAGGGTCCCCCTTGATTCTTACCCTCGCGCTCGCTCAGTCCGCGGTAAGCCGTGAAAGCCCCCGTGGCGGCCGCTTGTACCTATAGCGACCCGGGAGGCGGAATGTCACGGGACAACCCTTCTTGAGCAACCAATCAGAATCCCCTAGCAACGCCGTGCCCTCCCTCTATGCCCCAGCTCCCAGCCTCCAAAAGCTCCGCCCATCTAAGCTCGCGTTCTGCGCGTTCCCGAGGCGCGCTCCCGTGGGAAGTCGGCTTGGGCGCCCTGTGCGCGCGCCGCTGTGCCTCGGGTCTGGTTGGAGTCCTCCGTCCTCCGCCCCAGAGGGCGGGGGTGGTGGCCCGCACTGCCCGTGCTCAGTAAGTAGCTGTGCAGTGAATTCCTACAGAGGGCGATACCTTGCTCTTTTTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTTATTTTGTTTTGTTTCTTCGAGACAGGGTTTCTCTGTGTCCTGGAACTCATTCTCTAGACGAGGTTGGCCTCGAACTCAGAAATCCACCTGAAACCTTGCTCTTAACCACTCGAAAACGGTAGTGAAAGAGGCTGGGTGGGGTTTCAGCTCTCGAAAGGTCAGGAGGCTGCCCCCTCCACCTGACACCCAAGGGGTTGTTCGCCCACCCACTCCCCAAATGTATTTATTCATTACTTATTCATATGTTAAACACAGGCTAGGTGCCAACCACTATTTCTGGATGCCTTTACACCGATGATGATCATAGCTGTCCCCTCTCCGAATCTGTGCCATTTTCCTCCTATCAGGTAATGGCAAACCGACTTCTCTGCCAATCTTTTCTCTCAGCCTTTACATGCAATTGTCCAAAAATTGATTCCTGCCTTTAAATTCCACCTCTCATCCCACCCAGTAACGCTGGCCTCATTACATATTGCCTGGATGATTCGGAGACAGCCCTCAAGGGCCTCCTTTGTCCACCTTACTTCTCCAAGGCAGCCAAGGCAAACCTGAAAACTCAGCTCTGTGGGCTGGTAAGATGGCTCAGTGGTTAAGAGCACTTAGTGCTCTGGCAGAGCACGAGGGTCCGTCTCCAGACTCACATGCCAGGGGATCTGATGCCCTCTTCTGAACTCTGAGGCCACCAGGCATTCACATGGTGCACATACAGGCAGATAATCGGGCACACACTTAACCTAAAAAAACGAACCCAACCCAGCCCTGGTCTTGTCTCTCTGAAGTCTGTGTGATGGCTACTCTCTTAGTTTGGGGAAATGGTGTTTCTAAACCCCACCCTCTACCTTTTGACCCTATTCCCTGCCGTCATCTTCCTCATTGCCCTCCTTGTGGCCCCTCAAACACAGCCTTTGAACCAGCTGTGCCTTCTGCTTAGAATGCACTTCTAGATATGGGCATAGCCGACACCCTCATTTCCCTTAATTATATGTCCAAGTGAGGTCTACTCTGCCCATTCTTCTATTTAAAAACGGCAAGCTCTCCCCTCCCTCCTCAGGAAACTCATGCTGTATTCTCCCCTTACCCACCCTCCCGCGACCTTCACCTTGTAACACATTTACTCTAGTGGATACTACTAGTTGAAGAAAAGTTAGTGAACATTGCAGCCACCAACCACCACACTCCCCCCTTAAAACATCCAGCAAAATGTAGGGTATGCTTGTTTACATTGTGAGGTCAGGGAGGAGACCTCAGAGCGGACAGCTGAGACCTAAATGTCAAGGAGCTCTAAGTGGGGGTAGGGTGAGAGGGGAGGGCCGGCTGGGCTTCAGGAACTGCAAAGCAAGGGTTGAGGTGGGAATCCACAAAGCCCGTGGGCAGGCAGGCAGGCATTTGAACACAGTTGCATTCAGGGCGACTACGGGAATCCTGGTCTCCTATCTTATTAGTGCTTTCCCCGCAGCCTTCCCTGCAAGCCTATGGACAGACTCCAGGTAGAGCCTCAGGACCACAAGTGGACTGCAGACCTGCGTTGGTGGGTGATAGAAGCCGAAATGGCCAATATCTAGAGGCAGAGATGGGGCTAAGCCAAAGACTTGGTTGAGAACCTTAGCAGGGTTTTGCAGGATGTGTAGGAGCTCGCTTTAGGAAGCGCCCTATCTGATGATTTGTCTGGTTTGGCTCTGCACGCGCGAGAGGGAAGAGAGGGGCGGATCGGGGAGATACTGGATGGGCTGTAAGGGGAAACTTTTGGGGGAAAAAGAGCACTCCTTATTCTTCTTCGGTGTAGAGCTGGAGGCCTGTGGGTAGCCTGAGGTTGGGCTTGGGGTTCGAGACGACCCCTCACCGACGAGTCCTGTGGCGCAGGCAGGTCCCCACCTTCCAGGGCCTCAGTTTCCCTCCAATATGCGAGCCGAGGCGACTTTGCGGATCTGGAGGCCTGGTGGGTACTGGGCGGGGAGCGGCCGGGGCGGTACCCCGAGCCTCCCGGGCGGGGTCTCCCGGCTCCTGCGGCCACGTTTGCGACCTGGGCGGAGGCCGGGGTTCAGAGCGAGAAAGCCCAGGGGGCGACAGCATCGCACTCGACGGGCCAGGCCCTCGGGCGCCGGAGGTGAGAGCCGGTCAGGCGGGCTCCGGGAGGAGGTGACCAGGAGGGCGTGGCCGTGGACGTGGCTGGAAAGCCCGGGCGGGATCCAGGGGACCCAGGCACCAGCAGGGCTGCCGGAGGGAGAGACCGCGGCCGCAGGCGTGCGTGGACTCAGCCCCTCCTTGGGGCCCACAAAGGGCAGCGGCTAGAACAGCCTTCCTCTATTCCAGGCCTGCTTGCTTCTCTCTCTTTGGAGCCCTTCTTCCCCTCCCGCCTCCTGGCTACGTTTTTTATCACTCACTACTTGATTAATTCATTATTTATTTAATTGTATTTGCTTACGGAGTGTCTCTTTTGCTGGAAAGTAGATTTCAAGAGGACGGGGACCTAGTTTTGTCTACTGATGCATTCCTACAAATTCAGCAAGTCTGCACAGGGCTGATTATTGGCGATTTAACCACTCTAGATGTCCTGCAAATAGGGAGGGGAGGTGCGCGAAAGGGACAAAGCCAAATTCGATCTTTGGGTACATCAGTAATCAGGAGACTTCTCCCCAATATCGTGGTGACGGAAATCCCATAGGGACAGCCAGGAAATCTTAACTGCACTTAACTGACCTACCTAGCCCCAGGGAGCCCAGAATTACAGTCAAGAGACAGTGGGCCAAGACATACAGGGAACTTCAGTTGCCCTACGGGATGAGCAGATTATGAAAAGGAGGAGCGCTTCCGGCAAAAGGCTGCCCTTTTACTGGGGCCCTGAAGTTAGAACAGTTCTCACGTTCTCTGAGAACCCTTTTGCAGGCTCTCTGGAAACCATGTGGCTGAGCTGTGTGGTTAGATAAGAGGCCACCCTGTCCCCTTCTGTGACTCTGCTGATAAGTGTGTCACCAACAAGGCCCCACCTAGAAGTTATAAGACCAAGTGCTGACTCTTCCTCATATGACCCTTGCCAGTTCAGCTGTATAGGAACCTTGGAGCACCAGAATTTAGTGGCTATGTCTTCAGTGCCCCAGGACACACCGCTGGAGTTTACAGAGCCGGTCCCATTCACCCCTGGTCACCTCCTCCTTCCTCTGGTGCTCGTTGGTGTGATGTGGTTACCCCTAGGTGGTCTCGGTCACTGGCCTGTCCCTCCCCTGCCTCCATCTGGCTCTGAAATGTGGGCTGGATACCACTGGGGCTAGTAGGGGCTCAGGAAAGTCGTGGGCTCCCTGGAGGTTGAGCTTACACTTGCAGTGAGGGGCCCCATGTTTAGGATGTTTAAAGCACAAAAGTGTCTTTGGGCCTAGGGTCCCTTTCATCCCTCCCCAGGGGTTATTTCTTTGGGTTAAAAAAAAATGTCAAAAGGAATATCCAGGTTAGCCAGGGATATGGTATGACCCAGCCTGAAAGCCATGATTAAAAACTACAGCCATACCACCCTGAACACGACTGATCTTGTTTGATCTTGGAAGCTAAGCAGGGTCGGGCCTGGTTAGTATTCGGATGGGAGACCAACTGGGAATACTGGGTGCTGTATGCTTAAAACAAACAAACAAACAAACAAACGAACACCCAGAAGAGCTGGGGCTCCGTGTGCTCAGGGCTCCTGCCTTCAAAACCTAGCATGACAGAAGCTGAGCATGGGGTAGGTATGCAGGAAGAACTGGGCAGATGGGGACAGTCAGCTCAGAAGCTGAATTCAGATCAGACATCCTTGGCTACATAGTGAGTTCAAGAGCAGCCTGGGCTATGAGACTCTGTCTTCAAACAAACAAAGCACCATGTTGGGCTCTTCTCCTGATACAACTATTGTCTTTTTTGTTACAGTGTTTTGATGTCCAGGTTCTTTTTTTTTTTTTTTTTAAATTTTTTTGGTGACCCTTATAAGACTTTTGAGATGAAAGGCAAGGAAGACTAGTCCTTCAGCTGAAGTCTGAGAACTGCAGGAACATGGTCTGTAGGAGCCACAGGACTGGGCTGCTGGTCTTCTGAGCTGGGTCCTTCCCTGCGGGTGCTACCATCTCTCTATCTCACTGTTCTCGTGGGAGCCATGAAGCTGAACAAGAGGAGTGTGGCCCACTATGCGCTGAGTGATTCTCCGGCAGACCACACTGGCTTTCTGCGTAGTTGGGGAGGACCAGGTTCCCCACCCACTCCCAGTGGCACTGGCCGAAGATACTGGTTTGTTCTCAAGGGCAATCTGCTATTCTCTTTCGAGACTAGAGAGAGCCGGGTCCCGCTGAGTCTTGTGGTATTGGAAGGCTGCACGGTGGAGCTGGCCGAGGCTCCTGTACCCGAGGAGTTCGCCTTCGCTATTCGCTTTGATGCCCCTGGAGTGCGCCCACACCTGCTGGCAGCAGATGGGCAGGCTGCCCAAGAGGCATGGGTGAAGGCACTGTCCCGAGCCAGCTTTGGCTACATGCGACTTGTGGTGCGAGAATTGGAGAGCCAGTTGCAGGATGCCCGTCAGAGCCTGGCCTTGCATCGCTGTGCATCCCAGAGGGCTGTTGCCAATTGTAGTAAATCTCAGGCTCCTGACCACCGAGCTCCAGACCCTGAGAACGGCCACTTTCTCCCCAGGGACCGCAGTTCCATTGGTACTGTGGAAGAAAGGGGGATCCGGCCAATAGGTCGGGATTTGACCGAGTGGGAGTTACAGGGCCCTGCTAGCCTCCTCCTAAGCATGGGACAGAGCCCCGTGTCCCCTGAGAGCTCCTGTTTCTCTACCCTGCATGACTGGTATGGGAAGGAGATCATGGAGCTGAGGCGAGGGTGGCAGCAAAGGGCAAAAGGAAGCCAGACAGAGAACAAGTCACAGAATAGGCCCTGAGCCAGAGCCCTTTAAGCTATGGCCCTGTCCTGCTGGTCAGGATGCCGGGGTCAGAACGTCAGGGGTTAAATATTTACTCATTTACCAGGTTGCTCTGTGTGTGTGTGTGTGTGTTCCCCTGCTCTGTAGACTTTCTCTAGCTTCCAGATCCTCCCCTCTCGCTGGCATGAACTGAAGAATCATGGCTCCCTACTTATTTTCTGAGGTCTAGAAAGGCCAGGTGGTTGATGGGAAGATAATATAATTCAGGAAGTGGGGCTCTGCTCTCTAGCCATAAAGTCCAACACAAGAAGTCTTGGGCATCTCCATGCCAGAGGTGACAGGAAGTGGCTTGAAGACTGACATCGTTGTAGCTTGGGCAGGGCTCTCTGTAGGAGAAGGTCTTTAGGTGCTAAAGAGGGATTTGTTCTCTCTCCCGCTGCTGCCCTGTCCATGTATAGTGCTGGACATTGCCCGCAGGGAGGCAGGCAGCCTTTTATATAGAAGACCTGACCTGTCGTCATGGCAACTTGTAGGGACTCTTGTTACCTAGCCCGCTTGTTCCTAAGGCTTGTCTGCTGAGGGCTCTCATAGCTACTCTGTCCCGTGTCATTCCAAGTATCTTTTTCTTTAGCTCTAAGGTCAGATGGCTAGGTCTACAGAGCCAGAAACCAGGCCCGTCTGTCCCCTTTATATAGATGTAGTGACAGTGGCTGTGGCTCAATAGAGATGTATACCGTTCCCAAACAAAATTGGACTCTTCCTGACCTCCCTCCTTTCCCTATCTGGGGCCTTGGGCCAGCCTGACCCAAATCCAACAAGGCCTAAAGGCTGGATTTTGCCTCCAGGAGACTGAGTTCCATGGTTCCTCTGGTCAGGGCTATGTTTCTCAAGCCTGCTTGTAACCTCATAGCCAGCTGCCCTCACTGTACACACAGGTACCTTTGCTAAGTTTCTTGTCACAGTAGTTAGTGTTTGCTCTTTTAATAGTTTTGGTTTTTGGAGACCAAGTCTTTGTAGCCCACGCAGGTCTGGAACTCCTTAGCATGGCAATCTGCTGGGATTATAGATAGGAGTGCCACACCTCACTTGGTTTTGGTGTTTGTTTTGCAGCAGGACCTGGTGGAAGGCCTGTTAGCCTCCCCTCCCCCCTTCCCTTTATAACCAGAACGGTAAAGTTCCCCGGTTGGTAGCAAAGAGGTGGAGGAGGATCAGGAGTTCAAAAGCATCCTTGACAACACCGCGAGTTTTTTGGCTAGATTGGGCTATGTGTGAGACCCTCTCAGAAAGTTAATTTTGGTTATTACAATCAACGTGTTTACAAATGAAGAATCAGGAGCCTGAGTTGCAAAGCTGGTGTCTGAGGCTAACAATTGAGTCCAAGAACCTGGCTTGGTGGTATGACCTCCATCAGGCCAATCCGTCTATTTGTTTGTTCCCTAGATCTGTAGGGGAAGCCCGCTGTCCACTGCACCCATTGTACCAATGTCCACAGAAACCATGAGCTATTTATTGTTAACTTGATTTTTTTCGCCGCACTACGTAGCTCCGGCTGTCCTGGAACTTACTGGATTTGAACTCACAGAGATCCTCCTGCCTCTGCATCCCAAGTAGTGTAAGATTAAAGGTGTGCGCCACTTCGCCCAGCTTTTTGTTAACTTTTTGTTACCTTAAAAAAAAAAAAAAAATATATATATATATATATATATATATATATATAAATTCAGATACCAAGTAGAGTTTTTCATTACACATTCTGGTTCCTTAATTCAAGTCTTCCCGAGGTGAGGGGCGGGAACACCTACAAAGGGAGCCCTGGCGGCCCCGCCCTCTGGAGTCCGGGAGGGGCGTCCTTCTCCTGACGTCACTACGTCGTCCGGGCGCCGAGGACTTTCCCACCGGCATACGAATGCGTGCTCTGCGCAGGCGGGAGGGCGGTGCTTCCCGTGTGTGTCACGAGTCTCGCGAGTTTTGGAGAAAGTTTCTGCCAGAGGCCGCCTTGGATGCGTGGGGTGTGGCTCCCGGCGAGGGGCGGAGCTGGAGATGGCGTCCACGGCGGCTCGGCGGCTGGCCTGGGTTGCAGTTCGACCCGGGGCTCTCTGGAGCGGGCCGAGGGGGAGGAGAGGTGGCGATGTCTACACCGTACCGGGCAGCTCAGGTCTCAGCCAGGTACCGTCGAGGTCAGTCATCGTCACTCGCAGCGGCGCCATTTTGCCCAAGCCGGTGAAAGTGAGTGTCTGTCTTGAGGCGGGACGAAGGGATTGAGGCCGGTGATTGGGGGAGTGCATGTGACCTGTCCTGATTGACAGGCGCAGAGGCCAATCATAGTATTGGCATAGACTGGGGACCGGACATAGGACTCAATTTGTGCAGCTGGGTTTTGCTGACTTTGACAGGTGGAGGAAAATGTTTCCATAAAACTCACTGTTAAGCTGGGTTCCTGGACACAGAAGCTGGAAGGCAGCAGCGAAATGTGCAATTTCTTATCTTGAGGGACATAATGACCTATTTCTTCAGGGTCAGAAGAAAGGCTTTGTGTCGATTTATACAAGTCATGCAATCTCTGCCTCAAAGGTGATGGGATAAAACAATCCTGAGAGCTGGAGAGATGGCTCAGAGCTTAGGAACTCTGCTCTTCCAGAGGACCCAGGTTTAACTCCCATCACCACAGGACAGCTACAACTATCAGTAACTTCAAGATCTGACATCTTCGCATAGTCATACACGAGGAGGCAAAATACCAATGCACATAGAATAAATTACTGAAAGAGTGGGAAGGGAATGCGGAAGTAATATACTTAGTAAAAAGGAAATGAAGGAGAACGGATAGGGGGATGTGGGAGCAGGACCTTCTGTGCTGAGGAATGTGTAGGTTTTACAGATGAAGTCAGGGCAGAGCCCTGAAGCCAGCATCCAAAGCTAGGAGTTAGGTCTTGACAACCAGAGACCACATCCTGGTTTTTCCATACTTGAACTCTTGTCACTTGCCCTCGGTACCCCAAAGGAAAAATGGGAAGTCTCTTATACCAAAGGTGTGCTTTTTGACGGCATGGCCGTATGGAGTCGAAGGGAAAACAAAAGTCTTTAGAGCAAGCCTGAGCGGTAGCAGTTGACCCCAGTCAGGGTTCCAGCGAGAAGCAAAAATGTGCAGCTGCTGTATAAAAAGTCACAGCTAGGGGAAGCAGGAAGCTAATACCGGTGAAATCTCATTGGCATTCCTTCCGTCCATGTTGCCTTTGCGAGCTTTTAACATTAATTGTGAAGCAAGGTTGCTCAGCAGCAGGAGCCAGCTAAAAGAGAAGGAGGTGAGAGGCCCCATCAGTGCACACACCTCCTCCTAACTCGAGTTTTATTTATGTGTATGCATGTTTTGCCTGCATGTATGTCTGTGTAGCACTTAAGTGCCACATGCCCACAAATGCTAGAAGAGACTGTGCCATATTGGAGCTGGAGTTACTTGCGTGTGAGAGGCCACGTGGGTGCTGAGAA
D3_15 82178636 82193686 15051 TTAAGGTCCTCAGTGGCCTTCTCATTGTCCTTGATGGACTTCAGTCAACTGACATTCTCTTAATGTGCTTTTTATTTCATGAAGTCATGCAATCTCTGCCTCAGAGATGGAGGGATAAAACAGCAAATGATGGAGGAGAGGAAAGGGAATGCAGAAGATACCAGTAAGGAGATGGGCAAAGGGTGATCCAGGAAGGCATACCACAGGAACTCTCACTTCCTACTTTACATTGTTAAAGCTGCTTTGTATGGTTTCTCCTCCTCCCCCGCTGTTTCTTGAGTTACCCACATCCTTACCCATCTCCAAGTATTGGCCCATCCTCCTTCCACCGTGTAATCCTGTCATCTTCCTTTACCCTATTTGGAAGTGACTGCTGCTAAAGCCTGGTGTGGGATCTTGAGCTTTTGTGATCCCAGCACTGCAGAAGTAGAGGCACAGCTCAGGGGTTAACAGGAGAACTTATCTCAGAAAACAGAAATGCCCCAGGCCTGGTGGCACACAGCAGTAATCCCAGCACCGGGGAGGTGGAGGCAGGAACACCAGAACTTCAAGATCCTCCTTGGCTACAGTGGGCTTGAGACTACTCTGGGCTGTGCGAGGCTGTCTTACAAGTGACTGATGCACGCCTTTCTTACAGGGCACAGGGAGCACAGGAGAAAGCACTGATGATGTGGTGGCATGCTCAGCTCCCTCCCGTCGGCAGAAGGGTCAAGGAAAGCCCTCAGCCTCACCTCCTCATGGTTTGTGACAAGAGCCCAGAGTCCTGTGCTTTGGGAGGCTCCCTCACGGCTGTCCACAGAGTACTGGAAATAACAAGCAAGCAAATTAGGAGTGTATAATAAATGAGTCATGTATGATGAGAGGTCGTCCCCATTTCTCACTCCCAGCAGGCTTGGTTGCACTTTATTGTCTCTTCCGTGTCCAAGAAGCACCTGGTGTAGAGCCAGGACATCCAGGCTATTGTACAACCACAGAGGCAATTGAGGAGGACTGAGATCTTTCCTTTAAAATGATTAACTATGTATCTGTTTTCAGGTGCTCTAGGAGGCAGCATTCCCCCTAAAGCTGGAGTTAAAGGTTATTATGAATCCCTTGATGTGGGTGCTGGGAACTAACTCTGGTCCTCTGCAAGAGCAGCAAGTGCCCTTAACCACTGAGCCATCTTTCCACACACACCCTCCTCTGACTTGAAATTTTTCTACTTTCCATAAGTTCAGGAGAAACTTGCTTCAAAGCCACTTAGGCCTGGAAGGGTTGCCATGTGCTGAACATGCAAAGCTTTGTTAACTGCTTAGCAGAATATTTGATAACACTTGGGCCCCTGGGGGGGGGGGCGGGGAGGATCCCTGGTGCTGTGGGGTCATTCAGTAATTAAACCCCAATACTGTGAGTGCCTTTCTTCAGCATTCAGGACTGTCCAGGGAAGAAGGTCGAAACAAGAACATCGGTAGTTCAAGGTCATCCCTGGCTACACAGGGAGCAGGCAGGCTATCCTTGGCTACATGAGATCCTGTTTGAACAAGAAAGTGCATTAGTAGTAGGACCAGTGTGTTGTGTTTGCACTGACTAGTGTGCTCTGCAGTGAGGAGGCAGCTAAGCTCATTTGCAGACCAGTGCTCCTAAGCAGAAGGGCTAGCCTCCGGGCAGTCAGTTCTAGTGCTGTCCCCACAGTGCTGCTCTGTTCCCTCTGATGGAAGAGCCTTGCTGCCAAGTCTTGGAAGCTATATACATAAGTACAGAGACAGTAACAAAGTTACCATTTCACATCCACTGTGGGAGAATCGGATAGAGCCTCCCATCCCTTCCTGCTACCCCATGCAAGGTCTGAGTCAGCTGGCAGCAAACAACATAGATCTCCAGGACAGGGGCATCAACATGGTATGTGTGTACAAGAAGGTTCCACACTCAGGCAAGAAGTAAGAGAGACCACAGGAAAGGGGTTCACCAGTGCAATTTTATCACAACCAACCATCAAATAATGAGTTTATTTATACTCTAGTTAAATATTAACATGCACCTTCCCATCAGGTGACCTCATGGGTCATGGCCCATATAGAACTTAGATAAGAAATCCTTTGGCCTTGGTGTTTCTGTTTCATGGAAAAACCGCATAACGTGTGTCCGCTGCTTCCATACTTTGATGGTTTCCTGGAGCTCCATCTTTCCCTGTGTACATGAAGGAAAAGTAGTCAGTTCCAGCCATTAAATGAGGAACACAGCACACAAGTTCAGTTCTGTGTTGTGTTTGGCATGGACTCGCTAATGACCGTACATTAACCATACCCAGAGCCACACTGCCTTCCTGTGGTTGCAGACAGGTGAGACAGCGTGTGTTCCAGGGTGGCATCCCATAGGAGCTAGCAATTCCTACACTTGACACTGTTATAGCTGCAGCATGTCCCTCCTCCCTCCCCCAGACCTCCCCTTCAGGGCCGAGGCAAGAGGATGTGTGGTTCCAGCCCAGGACTCTATCTCAAAAGTGGAGACATCCAAGTGGGGTATGGTGGTACACACTTTTAATCTTGGCACTTGGCAGGCAGAGGCAGGTGGATCTCAGGGCTAGCCTGGTCTACAGAACAAGTCCCAGGACAGCCAGAACTACATACTGAGACCTTGGCTTTAAAAAGAAAAAAGGGGACCTCCAAGTTGAACTTGGAGACACGGCAGCTGCTGAGTCTAGAGCTGGCTTTAATGGAGGTGGTATTCACTTGAAGACTCAGGGGCTCAGGACAAGCCAGCGGCTCTTTTAAGTCTGCCAGAGAGGGCCAGTTACCTTAATGACCAGCAGATCAACCACTCTGGGGTCTGTGACATGGGCATTCTTCATGAACATTTCTCGGACTTTATCCCGTCCTTGTTTCACCGTGATATCCAGCTGCATTAAGTGCACTAAAGAGAAAACTGGACTGTAGAAATCAGACCTTTTAAGCATGGTTTTAGAAGCTTATGTCAGTGGGTGACCACTGGACATGTACAAGTGGCTCAGTTACTTTATTCCGCATAGCCCAACCCTAGCCTGGATACATGCCTTACTAAACTGCCCAACCTGGATCTCAGTGCTCTTGAGTGGGCTTCTGGAGAGGCTGTGTACCTACCTTGCTTTTTGTGTATGGGCGGAGCAGGCTGAGGCAGAATCTAGGGCCTTGCACATGTTAGGCAAGGGCTCCATCACTGAGGAATATTGTTTTTATATGGGATATAAAATATACTACCATATTATTTTTATATGGAATAACCAGCAAGAATAAAGGCACCAGAGTTGAAATCTCTGTAAAATAATGTGTAGGTGTTAAGTACTATATGAATTTGGGAGCTAAGGGGTACTGCCAAGAGTTTGCACAGGGAGGGAAGGCTGTAATCAAACCATCAGACAACCTGGGGAAGGACATGACTCTAAGCCTTAACTGAGAGTGGGACAGTCACCTATGACTGCAAACAAGATACAAGGCTGCCACTGTGGAGCCATGAGTCACACCACCCTGCACCACCACCCCCAGATAAGGGACTGAAACCGAACACAATAAACTTCTCTTCCCTTAAAAAGGGAGGAGGCTGAGAGGTGGCTCAGCATCGCCTTTCCAATCCCCAGCAACCATGTCAGGCAACTCAAAAACCCTGTAACTCCAGCTCCAGGGACCCACCCATGCCTCTGACCTCTGCGGACACTTGGGATTTACCTGCACCCTCCCATACACACACACACACACACACACACACACACACACACACACACACACACACACACACAACTTTAAAAAGTAATAAAATACATTTAAAAAGTCACAGTATTCACCAATCTATTAAGTCAGTGGCTAAACAGTGGCACAAACTCAGGTGACTGAACTTAATGTGCTACATGGGTGTCCTTTGGAGATTAGGTTGTAAACTGGACTTAGTGGTAACAGGACTGTAAACCCAGCCTACAGGCTGGAGGTTTGCCGTGGCTGTGTATGGTGCTATGAATCCACAGCAAGATCTTGTTTCAAAAGAGAAAAGCAGGCTTTAGTCACCCATCCCCTGACACTCAGCCTTCGGCAGATTTGCTGAGGACACCAAGGATACAAAAGGATCACTAGGTGTTAAACATTCTGGCTACAGAGGTCAGTATATTCATTTAGTGAAGATACCTTGTGCACTGGCCATTCTTGTGACATGTCCACTCTGATCTATAAAACTGCAATCAGCCAACCATGGTGGCAGATGCCTTTAATCCCAGAATTGAGGCAAAACCTTAACAAAAACAAAAACAGTGCTGGGCATGGTGGCGCACGCCTTTAATCTCAGCACTCGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCTAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTATACAGAAAAACCCTGTCTCAAAAAAACAAAAAACAACAAACCAAACCAAAACACAACACCCAAAAACAAAACCAAGCCCGGTGTGGTAGCACAGAGGCAGATGGCTCTTTGAGTTCAAGGCCTGCCTGGTTTACGTAGCAAGTTCCAGGCCAGCCAAGGCTACAATGTGAGACGCTGTCTCAGAAAAACAAAACAACAAAAAGTCACAAAAACACAGACAAAATCATGCTGTGTCTAAATCAGGTTTGAATCTTTTGTTATACTCCAATTCAGTGATTGCAGAAATGGTGAACACTAGGCTAGGTTCTGAGAGAGCTGTCACCAAGGTCAGTGCGCACACAGGGATTTCAGGAAAGAACTGTACAATGACAAACTGAGACAAGTTTAAGTTGAGACAGAACCCGTCACTCAGAATCTTAGTATTTGTGAAGCATCAGGCCCAGAGACTGAAGGGTGCAGTATTATAACTACTGTCAGGTAAACTCCAGCCTGAGGTGGGCATGGTTTTTTTGTTTTGTTTTTTCTCCAGTCTGTGTTCTAATAACTAAGAGGAATCTTCTCCCATCAGTGCCTCCCCCGAGTTTTATATCAGTTACTTAAGTAACCTATTTAATACAGTAACTCCCGGAAGAAGACAGTATTATGGCCATTTCACAGATGAGCAAATACCGGTGAAAAATCTTACCCAAGGTCACACAGCTAATAAAGGTCGAAACTGAGGACAGTGTGGCTGCCTCTCAAGCAGTGCTGAGGCGTGCTGTGGAGCTGTTGTTACTCATTGGAAAACGCCTTTCCCCTCAGATCAGCTCAAGTTAAATACATGTCTACTCAACAACAAAAGATGTCCCTGACATATGTAGGACATTTGAACTCTCGCTAACCCCCTTTTAAAATCCACTACAGTGGCAGCATTGAGGGAGACAACCAAGATTGCAGATTCACACTCGCCACTCAGGTGAACAGACTAGATTCTCCCACCTACGGGAAAAGGCGTGTTTTGCAGCAGAAGGGGGTTAGACTTCAAAATTCTGGGGCAAGCTGATCTAGTGAGCAAAGCTGGGGAGCTCTGAGGGGCCATTTCGTCTGGAGGCTTCAAAGGGCAGCAGGCAGCTACTGGGTGACCTTCGTCAAGTCGTTTCCCTCTCGTGATGCAGTTTGTTCTCCAGACTAAGTAACAGATAACTGCTAAAGCCCTTCGCCACTTTAGTTACAAAATCGAATGGGCATCTAGAACCTTCTCTGGAACTGTAAAGCTGGGTTCAAGAAGCACCCGGGGCTAGCTCAGGACCGCGGACGCCCCTCCCAGGGGCTCCATAAGAAATAAGGAACAAAGGCCACCAAAGCAACTGGGGCGCTCGGAAGCCCTGTCCCGGTGTGGGGCACTCTGGCTCGGGTGACCCCCTTCCCCCGAAGCCCAGGGCTCGTTTCTGTCCCACTACGTGACCTCAGCTCGGCCGAGAGACCTTGGGCAGGTGCCTCCGGCGCCCCGAATGCACCCCTGCCTCTCACCGGTGTTCGGCACCTCCCGATACCAAGCGCGGTAGAGCTCGCGCACCCTCCGCTTGGCCTCGTTCAGGTCGCGACTGAAAATGGGCTTCACCGAGGTACTGGCTGCCGCAGCAGCAGCTTGGCGCAGGCCGGTGGCCGCGGCCGCCATCTTGCCGAGGAAGCCCCGCCCCTTCAGCGTGCGCACGCGCGGTGCCACGGGACTCGCTGCCGTGCCTCCTGCAGTGCAGAGGGAAGGACTGACTGGACTAGGGCTCTCTGATTGGCTGGAAGCCTCGGGCTCTGCAATGCGCATGCTCTACCGCGGAAACACCGATCCGGGGTTGGCTCGTTCAACGGCGGCGCTGAGGAACCACCAGGAGGCGCAGCGTCGAGGCTTCAGGCAGCGGTGAGGCGCTCTGTCTGCTCTTACCAAAAGTTTGTTCCTTCAGCCAGGCGCCGTGGTTGGCAGGCTCGGAGGAGAAGACCTCGGGCATAGATAACGGTGCCAGGCGGCAATGGCAGTCTTTCCGCAGCAACAGTGCGTACTGCTGAGCATATTGTGTATGTGCTTCCGGAAAACCGTTTTCTTGTAGACATGGTGTCGATCGCAAAAGCGCCTCTGGCTTGAGCTTTTTGGCGTTTCTCAGCCCCGTTTGTTTACTTACTTCATGCCATTGTTTTGAGTTGTCGTCTCACTGCGCCTGGCCTGGAACTCACTTACGTAGACCAGCCAGGCTGGCCTCAGAGCTTGCAGTGATTTCTCTGCCTCTGCAGGTTTGTGCCCACAGCACTTGCGGTTGCCTTTTTGTAAATAAATGTGTGTGGGTGTTTTGGATGTTTGTATAGGTGTGCGCCACGTGTGCTTTTTGGAGTCCATGGAGGCCAAAAGAAGGCTATGGATTCCTCTGGGACCGACGGTTATGATTTGCTTTGTGGAGCTGGGAATTGAGCCCAGGTCCTTTGGCAGAGCAGTTAGAGTGCTTAAGCACTCACTGAGGCATCTCTCCAGCCCCGCAGTTACATTTGACCTTGCACTCCACATTTCCCCTGCTTCAGTCTCCTTAGTGCTACTTCACCTAGCCTAATCATTTCCATTTTAGACTAGGGATTTTACATCCCAAACCATGGAAGCTATATCTACCTCTGTATCTAATCTATATCTATACTCTATGGTATATATCTATATATATTCGCTGCTATATAAATAAAAGCTGAAGAATTGCTGCCTAACGACATTCTAATATAGTAAGTGCACCATGCTTTTATGGATATTTTTGTGGTGATTTGAACAAACAGTACTTCCAGTCAAAGTACAATGCACACAGATATGCTCAGCACACAGCATTTAAACTCTCATGGAGGAGAGCATGGCAGCAGGCAGGCATGAGTGGCCTTGAAGCAGCAGCTGAGGTCTTACAATAGAGAAAGACTGGGCCGGCCTCTAGTCACTTCTAGTGACACATCTTCAACAAGGGCCACATACTCCAACAAGGCCACACCTCCTAGTCCTTCCCAAAACAGTTCCACTAACTAGGGACCTAGCATTCAAACACATGAACTTATGGGGCCCAAACCACCACATTATTGATTTTTGTTTTTTTCTTTTATTCTCTCCCCTCCTTTTTGAAACCTGGCTAACCTGGGACTTTCTTTATAGACCCATGTTGGATGATTCACAACTACCATAACTAGCTCTAAGGGGCCCAGTAACCTTTTTTCTAGCCTCTATTAAGCACCTGCATAATGTGGTCCCTATACACATACACACATGAACACACACACACACACACACACACACACACGCACATACAGATAAATTATTTAAAAAATTTAGTTTTTCAGACTTGTTTTAAGGTCTAAAATGTAGTCTTTTCTGTCGTGACTAGTATTTCATGTGCATATAAACCAAGACATTCTCTAGTTTTGGCCATCTACAGGTACATAGTCGTGATTCAGCGACCTTTATGAGGTTTGGAGGAGGTAGGAATTGCTGTCATGCGCTATGCCCACTCACTTGATATCACTGAGTACCCTCAGAGGCAGGCTCTGTGTCCTTTCTGTGTCCCAGCATGCTGCTGTACCCCACATGGAAGCTCAACAAACACTGGCTGGGTGAGTGGATGGCACTTGGACGCTGCTAGGCTCCCTTCTTACTTATGATTCAGAGGATAGGCTGCTCGGTGTTAAGCCCTCCCATGTCACTGTGACTTCCATGTCTTCAGGACTTCACATGGCGCTCCCTCATCACCTCGTTACTGCCCCCTTATCCTTGAATGGCGTTGAACTGAACCAAAGGAGTACCAGTGAGGGTGTTACCCCTCGCCGGGCACACTTAGAACCACCTTGAGGCAATTGGCATGACACCCTTCTCAGATATGGAGGGGTTGTCTATCTGAAAGCTCTCTACATCACGATGCCACACCCTCCCTGCAAAAGGAGGGCCTGCGCACCCAACTCCCAGGCAAGGCTGGTATTGACACTCTTCCCAGGCTGTCTGTAGGCTACGTGGACACTGACACAAAGAAGTCTCTGGATCCCTGACTGTCATCCAGATGGGCGTGCTTGGTAAGTAGGTTTAGAGGTGTTCTTGTTAACCCATACCCAACCCAGTGAGAAGGATCCTTAAGGCCCAGTCCTGCCCTCTGTGTATCCCTTCCTTCTTGGCTCTCCCCTTCTGTGTATCCTTCTTGGTTATCCCCTTCCTCCTCTCCAGAACTGATCTTACTTCCTTTCCCCTCTCCTAGCTTCCTTGCAGGATGAGCTTGATACCGTACTCATTGACACGGAGTCCTGAGTGACTCACAGAACTTAGAGTTGAATACACTGCTAGCAGCACTCTCAGTAAGATGTGGTGGCTCTGCTTTAAAATGCTCACCAGGACTTGAAGCAGCAGATAGGAACAGCAGAATCATGCAATCAGTCATGTTGTTATTGATCTGTCTCAGCCATAAGACCAATTTTCTAGACACTGTTAATGGCTTCTCTAATAGGAGAAGCCCTCATTGGGAAACCAAGGGAGGTGTGCTGCCCCACCTCAGTCAGAGAAGGAAGCATTTCACAACCACTATGTCACTATGAATGTGAATTTTAGGCGGGTAAGGGAACAGAGAAGGACCTAGAGTTTGGGAGTTTGTATATATATTGAGTTTAGGCTAGCCTAGGCTACATATTGAGAGTCTCTCTCTCTCTGTCTGTCTCTGTCTATGTCTCTGTCTCTGTCTCTCTCTCTGTATATATGTCAATCAGACAGACAGACAGATCGTAAGATAGATGACAGGAGGGGCCAGAATGACCTCATACAACACTCACTTTTAGGCCAATCAGGGAAGAAGAACATGAGGGGTGGGAAGAAACCCAGAAGATGAGACATCTACAGCCAGAAGAGGTGATTCTAGGAAGAGGGTGACACCAGAGTGCATAGCATGGACAAGGGAGGCCACTGGGGTGGTGGCCAGAGTGTATCGGGGATGGGGCTAAGGCTGGCAACTTCAGGTTTCAGTCAACTTCAAGTGCTTCATGGATTCAAAGCACACTGAAGTGGGTTGTTGTATTGAGGGGAAGAGGGAGCAGTGCCGACATCCACTGTAGACCATTCCTGGAGGAGACTGCGCTGTGGGACAGTAAGGGCAGGAACTGGAAAGGATCTCAGGTTCCAGAGAAGTCTGGCTTTTCATGGGAGGGATGTGTGGTGTGTTTCAGCGGTGATGGAAGTGAGGCGGAAGAGGGAGAAGCAGAATGTGCAGGTGAGGAGGGCTCACCGGATGCTCAGCTGGTGAGAAGGCCAGGGAGAATGGGGTACCGGAGCCTTGGAGAAGACTAGGTCACTTCTGCCACCAGAAGAGAGGGCCCGTGGGAAAGTCAAGGAAGATCCTTCCCGATGGCACGCCTGTTCTTGGTGACACAGAAAACCTGGAACAGAGTTGGGGTGGAAGGTTTACAGAGGGAATCTGAAATCATCTCTTCAGTGTGGGCAGCTGATGAGGAAGTGGAAGGGTCTCACTATGCAGCCTTATAGCTTTGGCTGGCCTGGAACTCTTGATGTAGACCAGGCTGATAAAGACATGTCTGCCTCCGTGTCCTGAGTGCTGAGATTAAAGGTGTGTGCTACCATACCCCCAAATTATTTTTAAAGGGTACATAAAACTGAAAACACCTTAAAGTTGGTTGGTAGTTCTTAACCATTTTATTTCTGTTATACCCAAGGGCATTTTTTTTTCTTTTTTTTTTTTTTTACCATATGTTAGATCGATGCTTTCTTAAAATTCTACTACTGAGGGAAAAAAGGAAATCAACAAGGAAAAGACATGGCTGCTTCTAGGTATGGTAGAGGAGGTTTACTGTAGATATTCGGGAGAGAGTAGCCAGAGGTGGGGACATCTGGGAGAGTCCGGAATGGACATGACCCAGAGCTGTGGAAGGAGAGAGGGGAGGGGAGAGAGGAGAGCTAGACCAAGAGACCAGGAGGGTAAAGGGTAAACAGGGTAAACTTCAGAGTCAGGAGCCAAGTCAGAAAGGAAGCAGACATGTGAGAGACAGAGCAGCGTGGAGCTGGAAAGAAAACAGGCATGCGAGAGAGAGCAGCATGGAGTTGGCATGTGTGGGGTTGTGGTCACGTTCTGTGTGTTCCAGAACAGCAGGCTGGCGGTGAGACGCTATTTCCAGTCTTGGTTGTGTGGTCTTTGGGTCCAGAGCAAGCAGGCTTTGGAGGAGTGTAGGAAGACCGAGCCGATCAACAGGAAGTGAGCAAGTATGGCTGCCCAGCAAGGGTCAGAATCTGCTCCTTGCTTCCGGATCGCCCCCTCGGCCTGATGGACCCAGTAAACAAGGAAAGGAAGCACACAGGAGCTCCGTGTGTGCCCCACACCGCAAGTCTGGTAAGTTCGGAAGGAATCGGTAGAGGCATGCAGTCACGTCTGGCAGCCAGCAGAGCAGAGCCAGAGCAGAGCGTCTGGCCTGAGCCCTGTCGCAGCTGAGATGTGTTGGTCTCCTGTGGATTTTGAATGCGTGCATCGTGGTGGAGGAAGCTTTGGGGCCTGGGAAGGGTGAGGCTAAGCAGCCACGTGAGGCCAGAGAGAGGTCACAGCCTCATTTGGCAGTGTTGCTCTTCTAGATTGCCCAGGTCTCCTGTAGGCAGCACACTAGCACATGTCTTTATTCTCTCGGGTTGCTGGGCCTGGGTAATCAGTTCCCACCCACTGATCTGCAAACCCAGGGTTTCTCCAGCTTTCTTCTAAGGCTGCTGTGTACTTAATTACCAGGGGGACTGACACCTTTCTCAAGGCCAAGGCTGTCCGTTATTGATTTCTTCCCTCTGGACTCATACTGAAAGAGAACCTATTTTTCTCGAAAGTTCATGCTGCCCTGTAACCATGAACACGGAGCAAGTAAACCCCATACAACCTGATAGTGCCCACAATAGTGAGCGTTAGGAGTTTTGCTTGCTTTGGTTGGAAAAGCTATGTGTCTGCTCATGCATTTAATACTTGATATTTCCCCCATAAGTTTGAATGACTTTCAGGAATATTTTCGCTGAGTGTCACGGTATGCACCTGTAACCCCAATACTCAGGAGCCTGAGGTAGAAGATTTGGTAGGATTTTGAGGCTAACCTAACCTATGCAGTGAATTTCAGATCAGCTCAGGCTATAAGGTGGAAACCTGTTTCAAAACCCAGTAATTCTTTACACTGAAATGGAGAGGCAGGCATGTGCATGACTTCAGGGCCAATCTAGACTACATAATGAATTCTAGGCCAGCCCAAGCTGAACAATGAGACCCTGTAGTGGCCATTCCTGGTTGTCAACTTGACTATATCTGGAATGATCTACAATCCAGAACTGGAAGGTTCACCAGTGATCCTAATCTGGAGGCTGGGAGATACAAGTTTCTGACCTGGATTTTGGCATGGAGATCTTGCGGCATGGTGGCTATGAATCCCAGAAGATTAAGACAGGGAGAGCTCTGAGTTCAAGGTCATCTGGGACAAAGCAAGTCCCAGATCCCGGCATGGTGGCACACACCTTTAATCTGGGCCACACCTTCTGCTGGAGACCTAATGGAAGAAGGAAGATTTGCTCGCTCTCTTTGCCTGCTTGCCTTGTGGGGCTGAGCAACTGCTAGATCCTTGGACTTCTATTCACAGCTGCTGCTGACCATTGTTGGGGAGTTGGACTACAGATAGAAAGTCATCAACAAATTCCCTTACTATATATAGAGACTACCCATAAATTCTGTGACTCTTGAGAACCCTGACAATACAGATTCCATCTCAAAAACAAAAAAAAGAAAACAAAAAAATCCCTTAAAAAATATAACAAGCTAGGCAGTGGTTGTGCATGCCCCTAATCCCAGCACTTAGGAGGCAGAGGCAGGTGGGTCTCCGTGAGTTCAAGGCCAGCCAGGGATACACAGAAAAACTGTTTCTTCATCTTTCCCACCAGCCTGCAGGAGCAGTGTAACTATTTCAAGTCTATGGGGAAAACAGTTCTCGACTATGAGACTGCAGAGGCACTATATCACAGCTCAGGTATGGAGGCCAATGCTCTCATGTGAGCCTAGAGAAGACTCAGATCCTCATAGTCAGCACCTATGACATCAAGATGATGTGTAAGCAAAAGAGCTCTGGAGAAACTGGGCACAGCGATACACATCTTTAATTCCAGCACTTGGGAGCAGAAGCAGGCTGATCTCTGTGTGTTTGAGACCAGCCTTGTCTGTGGAGTAGATGTCAGGCCAGGCAGGGCTATCTAAAACCCTATAACAACAACAGAACACAACACAAAACCAACCAACCCCAATCTACCTCTTGAGAATTTCTCGTGTCTCCTTTGGCCAAGCTCAAAGCAGAGAGCCTTATCATCCCCCAGAGCCCCACCTCCCTCCCAGCCATTGGCTAACTTTCCATGGTAGTAGTCCAGACAATGCCTTTGTCCCAGTGCACATGTGTGGCTTTAGAATGTGGCTCAATGTTCATAGCTCAGCAAGGTAGCTAGTGTAACAAGGACACCTGTCTGGGATTAGAGTCTCCTTGATGTCCTAGATCCAAGAACTTGGGGCAGTACATAAACTTTAATGTGGTACTGGGAAACAACAAGCATTAGGAGTATGGGAGTCCTCAGGCCTTAGAGGGTACTTGGTGCTGAGTAAATTGTTCTCTTAGCGAATGATCACAGGGTCCACCTGTTTGTGTTTTAGAGGCTTCTATGGTCCTGCGTTTCCTGAGAAAGCTCTGGCTGGGGTTTCTTCTAGCCTCTGATCCCCTGCAAGGTGAGGTACCACAAACAAGAAAGGCTCCATTAGGGCCTGTTATCTGCTGCTTCTGAAAGGGCAGCTGCCTTCTTTTCAGGGTCCCAAGGCTGCTTTGGGATATTCATGCTCTCCCACCAGCTGCAGGGGCATACTTCCTGCTCCCTAATCTTCCTGCTCTCTAATCTCATGTGTACTCTTCAGTGACACTCGAGGGCCTAGGCCAGCCCTTTGACACAGTGCTAGAGTCCAGATGTCCTGGAACATGAGCTACACACATGCTCGCGCACGCACGCACGCACGCAGGCAGGCACAGTGCCACCGATCAGGGGATCAGTGGCAGAACTAGCTGGATACTGCAGGATCCTCTGGGGGAAAAAGTGTCCCAGGCTCTCTGTCAAGAGTGGAATTTTCTGTCCCAGGGTGTTATTAAATGCACTGAAGTGTCAGGTGTCTCCTCCCCACCTCCTCCCGTGCTCAGCTCAGCTCAGCTTAGTTACCATTCCATAAAGAGAAGTGACAAATAGCAGTGCCAGCTCTGGGCAGGTGCTTTAGTTACTCTGTAGGAGGGGACAAAGCGCCCAGCAACTGCAGGAAGGAAGGGTTCATTTAGTCTTTCAGTCTGAGGGTGCAGTTCAGCATGGTGGGAAAGGGGTGGCAGCAGAGGCCTGAAGTGGCTGCTCACACTGCTCCTATAACCAGGAAGCGGAGAGGGATTGTTGGCTATAACCAGGAAGTGGAGAGGGATTGTTGGCTATAACCAGGAGGTGGAGAGGGATTGTTGGCTATAACCAGGAAGCGGAGAGGGATTGTTGGCTATAACCAGGAAGCGGAGAGGGATTGTTGGCTATAACCAGGAAGTGGAGAGGGATTGTTGGCTATAACCAGGAGGTGGAGAGGGATTGTTGGCTATAACCAGGAAGCGGAGAGGGATTGTTGGCTATAACCAGGAGGTGGAGAGGGATTGTTGGCTATAATCAGGAGGTGGAGAGGGATTGATTGTTGGTGCTCTGCTCACTCTCTCCTTTTGCTTCAGTCCTGGACACAAGCCCAAGGTGCTGTCCATCTTAATTAACTCAATCTAGGAACTCCTCAGACATGGCCAGACATTTGTCTCTTTGGTGACTCTACATTCTATCAAGTTGAAACTATCTATCCCCCATAGCGGGAAACCGTAGGCCAGCCAAGGTCACAGGCCCAGGAGTCAGAGCAGGAAGGCAATCTGGTATAGTGAAGAATAGAAAATTACTGGCCTCTTGTGAGAACATGAACTTTTTACCTCGGAGCCCACCCCCTCCCATCTAGAGGTTGTTCTCGGAACACTCCTAAACTTTTCACCCCAAAACTCCTCACCCTAAAGTTCGAAAAAACTGTTCCAAGAACATTTTTGAGATAAAGGCCTCCTGAAACAACCTCAAAATGAACCGGGTACATTGCCAAATGATAGGACATGACTCCTTAGTTACGTAGGTTCCTTGATAGGACATGACTCCTTAGTTACGTAGATTCCTTTGGCAGAACTCCCTAGTGATGTAAACTTGTACTTTTCCTGCCCAGTTCTCCCCCTTTGAGTTTTACTATATAAGCCTGTGAAAAATTTTTGCTGACCTTCGAGACTCCTCTACCCTGTGCTAAGGTGTATGAGTTTCGACCCCAGAGCTCTGTGTGCTTCCATGTTGCTGCTTTATTTCGACCCCAGAGCTCTGGTCTGTGTGCTTTCATGTTGCTGCCTTATTAAATCTTGCCTTCTACATTTTATGTATGGTCTCAGTGTCTTCTTGGGTACGCGGCTGTCCCGGGACTTGAGTGTCTGAGTGAGGGTCTTCCCTCGAGGGTCTTTCATTTGGTGCGTTGGCCGGGAATTCGAGAATCTTTCATTTGGTGCATTGGCCGGGAAACAGCGCGACCACCCAGAGGTCCTAGACCCACTTAGAGGTAAGATTCTTTGTTCTG
GF3 -1 -1 1 CTCCAATATGCGAGCCGAGGCGAC
GF4 -1 -1 1 CGGCCGCAGGCGTGCGTGGACTCAG
EX52 -1 -1 1 GTCTTCTCCGAGTGTTCTCCATTGTGATC
EX32 -1 -1 1 CTCAAGCAGAGCAGCGAAGTTCTTGC
GR3 -1 -1 1 CGCGAGCTCTACCGCGCTTGGTATC
GR4 -1 -1 1 GTGCGCGAGCTCTACCGCGCTTGGTATC
Comments:
Created
Target: 0402Ensembl ENSMUSG00000022452 -- 82178330 -- 82178470 ENSMUSE00000127099 -- ENSMUSE00000127099
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: 15, start 82178330: end 82178470
Found 10 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:15:82171830:82172829_178 G3:15:82181970:82182969_741
ACCEPT (0) gap pair G5:15:82171830:82172829_178 G3:15:82181970:82182969_733
ACCEPT (0) gap pair G5:15:82171830:82172829_178 G3:15:82181970:82182969_728
ACCEPT (0) gap pair G5:15:82171830:82172829_173 G3:15:82181970:82182969_741
ACCEPT (0) gap pair G5:15:82171830:82172829_173 G3:15:82181970:82182969_733
ACCEPT (0) gap pair G5:15:82171830:82172829_173 G3:15:82181970:82182969_728
ACCEPT (0) gap pair G5:15:82171830:82172829_218 G3:15:82181970:82182969_741
ACCEPT (0) gap pair G5:15:82171830:82172829_218 G3:15:82181970:82182969_733
ACCEPT (0) gap pair G5:15:82171830:82172829_218 G3:15:82181970:82182969_728
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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