High Throughput Gene Targeting: Create Design

This custom vector design tool is provided by the EUCOMM consortium as a service to the research community. When using this tool, the user agrees not to submit more than five designs per day for creation without prior discussion with the High Throughput Gene Targeting Group at the Sanger Institute.

Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by am9
Valid After Annotation
yes
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 89538
Chr: 6
Strand: 1
Status: Ordered
BACs: RP24-283L13 : RP24-123I2 : RP24-288F10 : RP24-379H22 : RP24-401O13 : RP24-248E16 : RP24-495N13 : RP24-466K15 : RP24-459L19 : RP24-272J12 :
Instance: Plate 93: Well C03: BACs RP24-288F10 RP24-283L13 RP24-123I2 RP24-272J12
Oligos on GRCm38:
Type Start End Length Seq
G5 115941199 115941248 50 TGTGTGGACCTGTGACCTAGAACCTAAGAAATAGTATATGAAGGATCGAG
U5 115947141 115947190 50 TTTGTCCCCAGGGTCTTTGGTGCCTTCTTCAAGGTGACGTGTATGTAAGA
U3 115947242 115947291 50 TACAGCTACAGCTGTAGATACCCTTGGGCAGCTAACCTACCCGGGATTGC
D5 115948010 115948059 50 CCCGCACAGTATCAGGGTCTGTGTATGTGGCTTCTTGCCGGTGTCCACCA
D3 115948119 115948168 50 ATGTGGTACCTGTGGTTACTGGGGTGCCCATCTCAAGAAGTCCCAATCCT
G3 115951953 115952002 50 AAGACCACTATCCGAAGCTCTGCATTTGATTAAGACTACCTTGGAGAAGC
Oligos:
Type Start End Length Seq
D5_D3 115898078 115898136 59 CATAGCCACAGACCCACAGCTTCTGGGGGAGGGGCACTGGGGCAGTCTCTACTAGCCTC
G5 115891217 115891266 50 TGTGTGGACCTGTGACCTAGAACCTAAGAAATAGTATATGAAGGATCGAG
U5 115897159 115897208 50 TTTGTCCCCAGGGTCTTTGGTGCCTTCTTCAAGGTGACGTGTATGTAAGA
U3 115897260 115897309 50 TACAGCTACAGCTGTAGATACCCTTGGGCAGCTAACCTACCCGGGATTGC
D5 115898028 115898077 50 CCCGCACAGTATCAGGGTCTGTGTATGTGGCTTCTTGCCGGTGTCCACCA
D3 115898137 115898186 50 ATGTGGTACCTGTGGTTACTGGGGTGCCCATCTCAAGAAGTCCCAATCCT
G3 115901971 115902020 50 AAGACCACTATCCGAAGCTCTGCATTTGATTAAGACTACCTTGGAGAAGC
G5_U5 115891217 115897208 5992 TGTGTGGACCTGTGACCTAGAACCTAAGAAATAGTATATGAAGGATCGAGAAGACATCAGTATGAATGGCACTTCAGGCAGCAGCTGTTCAATGCATGAGGCTTCCCTGCCCAGGGATGAGGCCAGGGTTCAGCAGCAGCCCCGGTGGGGGAGGGGCGGGTGCAGAGATGAGCTTGATGACGACCCAGGAAAGGAGGAAGAGGGGCCCGAGCGAGGGGGGGGGGGGCGGGCGGGGGGGTTGGAGGCGAGGCTGGGGCGGGGCGGAGGAAGTTCTAAACTGAGCGCCTGACCCCATGCCACTATCACCTTCTCCTCCTGATGGCTTGGAAGCATACAACTCTGGGCTGTGAAATTCAGAGACCAGAAGGGCCCAGCACTTTCCTCTGCTTTCCTTGTTGGCTGGAATGTGATCGTAGTGACTGGCGCTGCAGCAGTCTTGTTGGATCATGAAATGGGCCCTAAGTCAAAGAAGAGACAGGGTCTGGCTCCACTAGGGCTACCTCGACAGTTGGGTCCTCACCAGCCAGGGCGACTTAAAGAAGTTCTGAAGGGGAGGCTGGCGACAAGAGAGAATGGCTGTTTGGGGATTACCACACTTATCCTTAGTACAGAGGACTTCCAGGATGATAGGGTCTGAGTGTGGGGTGAGCCAAACTTCTGGACTTCAGCCTCTTCATGTGTAAAGTGACGAAGATGCTGATCTATACAATTGTATAATGTCAATTATACGGTTTATACAGACATGCCAGTGGCTGCAGTTTCACTCCACCCTGATGCACTGATTAGGAAATCGATGTCCCAATGTCCTGATGGCACAGGATCCTCTGACCATCTCAGAGTTCGACATATTTATCTCTGAAAATGGAGCATGTCCTAGAGTGGAAGGAGAAGGTTCCAGAAGGAAGGCAGCATCCCCCCACCCCCGCCTGGTCCCACAGAGAAGTCAGGAGGCTGGCAGAGGGCACGCTTCTGCCAGCACCTCCCTAACTTGCTATCATCTCCCTGTCAAGTGGCAAACCCCTTGGAGAATTTTTAGATCTCTGAGTTTTTCCCAAACTCCCTTCCCAACACAAAACTCCATCCTGGCACAGCCTGGGAGCAGGGTTTCAGCAAAGCTCGTGGGAATGTTTACACCACAGAGTCTCTGCGTTTCTCAGTTCTTGGAAATGCCCCACAAATGGATCAAAATTGCGGCCCCCCGTGTGTGCCAGCTCCCGTTTGCCTCTTTGCCTCCCGGAGCGCCTTCTCCGTCTAGCACTGGGCATTTGCAGGTCTCTGGTCATTCCTTGGAGCCATAGCACCGCAATAAGCCTGGAAGGCCTACTGTGTGCTAGTGTTGGGCTGAGTGCCAGAGACAGCCTTGATCCACCACACAGCGCAAGAGTTAGGGACCTGTAAGGCAGTGCTGTGGCATTCCTGAGCAAACCTTACCTACATGAGAGAGATAGGAACAGAAGGCTTTCTAGGCCTGATGTAGTAGCAATCTTGTGCTGTGTGACAAACATTCCTAGAATTTACAGTTTAAAACAACAAGCCACATGTCATTTCTCCCAGCTCCAGGGATGTTGGATGGTCTTCTAGCTAGGGGATGCTGCTGACTGGGATCCAGCCTGGACCATCTCTGCTAGGCTGCTTCAAGCATCCATGGTCAAGTGAAGGACTAGCTGATGACCAGTTGGTTGCCTGGATGTTGGCTGTGTAACGAGTGACTGGACCACGGGTCTCAGCATCCAACACACTAGCCAGGGCTTGCTCCCATGACAATGGGATCACGATAGCACCCTTCCTGTGGTAGCTCACAGCACAGGGCTACCACAGATATAAGACTGAGATCAACGAAGCCTCATTGAATGTGTGTATAGATATGGGCAGAGATAGGAACTCTGGATCATTGCACATTAATTTGACACAGAAGCACAGAAGTGTGACATCCTAGTTTTAGCTATAGTTCATTCAAAGGTCACATGGTGTGAGTTTTTTTTTTTAGTTTATTATTGAAGGTTGAACCCAGAATCTCAGAGATGCTAAGCCCTCATTCAACCACTGAGCCCCAACCCCGAGTCCTACCTGCAGTGTGGTTGAATTGTGGAATTCAAGGGAGAGGAAAGCGCCATGAGCCTGATGGAAAATCTCAAACTTTTCCTCAAAGGAAGTAGGGACCCATGGGAGATTCTAGAAATGGAGTGAGGTCTGTTTCACTGCAAAGAACTTTGAGGGCCACAGCCTTTCTCAAACCAGACTTATGATGGATGGTGAGTTCTCTGGGAAAATTCCAGAAAATAGAGGGATCACACACACAGAGGGGAGAAGCTTGCAAGTAAGGCATCCTCAACCTTCACCTTTCCCAAAGCAGCTGCTGCTACCACACTGGTGTGAGTCCTGGTAGACCTGAGCTCGAGTCAGGGCCCTGCAAGGCCGTGCATTACAGTAATGAGCAGACCTTATCTACAGGAGGGAGCTGAAGGCCTTCTTCAGCGCTGGCCTCCCCATTCTCGCAGCTGTGTCCTATCTCCCTGTCAACAGGCAACAATTGACTGAGCCTGTTCCTATCCTGACAAGTTTTTAGTTTGTCTTGAATTTCTGATGATACTGGGGCAAAGTTCCCATTTGTTAGTCCCCCTGTTTTTCCCCAGGGAGGGATGGCTGAGCCGTGAAGCAAGCTCATCTTTGCTCCATAAAATGTTGAGTTGCCTCTAAGGTGGCTGGCCTGTCAGCTCTCCCAGCTTCTGTGTGATTCCCGTACAGTCACTATGAGAAATGCTCATCTCATATTTCATAAGCAATATTGACATTCAGCCAGGAGAGAGATTCAGCTTGTTGTAATCCATCCTGGTTTTGTTTCCTTTTGGTTTGACTCTTTTTTTGAGTCAGGGGCTTGCAGTCTAGGCTAGCCCCAAACGCCCTAGCCTCCTGCCTCAGTTTCCCCAGTGTGGGAACAAGAACAAGCCACAGTCATATATGCTGTAGCATGTTTTACAGCGATGGAGTCATTTACTCATGCCCATGTGGCTTCACTGAGAAGTCACTTCTGTCTTTCATCTTGATGACTCAACCCTTCCTAGGAAACAGGCAGAATGACCAGGCTCGGGAGATCCCTTCCCAAGTCATAGCCCTGTTGGGGGTGGGGGGGTGGCAGCTGGCTGGTTCTCTCACCCTAGCCAGTTCCAGTGTTGGGTAGTATGATGGAAAACAGTGACTTAGGTCTGTCCCAGTGTGTCTTGGAATGCCAAGGACACTGAAGACAGTGACCATCAGCCAAGGTCACATAAAGACCCTGACACACCTTCCCCTACATCTCAAACTGCAGTAAAATCTAAAACATCAAAATAACACAGAAGGAAGAGATCTGAGAGCCAGCCTGGCTCGCTCCGGAGAGGCCACTGCTTCCAAACACTCCCTCCCTTGGGCACAATTGCTGCTTTATGGAAAACTGAGGCTAGCAGTAGTCTGGATCAGGGTAGACAGATTCCTACCCTTGTGTGATTAGGGGGCCTTTGTGAGCAACCCCCCAACTCACAGTGTTAGATGTCAGCTCGTCAGGTAGGTAGGACACAGTGACACTGCCTCCTTACTTCTTCCCGTGGCCTCAGGTCCCACAGGGTAAGGTGGATGCTGTGTTCAGAGTGAGTCTTACCCTCGAGGCCTGATTGGCAGCTTTCGGCTGCTCAGGTGCTAGGCATGTTGGTCATTGGTTAAGTTCTGTTGGGAGGTATTTAAGGCAGAAGGTCTGGAGCTAACTGAGCTAACTGAGCGAGCACTGGCCTGGCCTGCTGCTGCCTGTGCCATGGCTCCTGGGAGTGTCTCCAGTGTTTCTTCCTCCTCTTTTCCCTCCAGGGACACATCCCCTTCTGGATCATGTGGGCTCCCTGGAGCTGACAAGCCAGGTAAGCAAGGGAGTGGAATTCTGAGTTTAGGCCAAGGTCCCTGGAAACCGGGAGACAGCCCGGAGACAGCCGCTGAGAAGGTGCATGCGGCTTAAGCCTGGTTCCCACCGCTCGCTCGGATTGGAAGAGGAGACCTAGTCCTGGTGGGGAATGAAGCTGAATGGTCCCATTTCCTGGTCTGGGACTCACAGTGAAGACTTCAGTCAGGTGCAGGCTGCCTCGGCCCTGCTACAGGACTCCCTTAGGCCCGTGGCCTGTTGTTTCATGTTTCTTTCTTTTTTCTTTTACACGCATTGGTGTTTTGCCTGCACATATGTCTGTGTCGGGGTACTGGACCCAATGGAACTAGAATTTCAGAGAACTGGAGTTCAGACAGCTGTGAACTGCCATGGGAATTGAACCCTGGTCCTCTGGAAAAGCACCCAGTGCTCTTAACCACTGAGCCATCTCTCCAGCCGATGGCTTTTTCTTTCTACTTCTAAACTTAAATTTTGAGTCTGAATTTTGCAAATAATTCTGACAGCATCACCCTGAATTTAAGTCACGCCGACAGGTTCTCCCATTTCTCCCCCCCAAATCCTCCTTTTTCTTGCTGCTTTTCAAAAGTAACAGCCCATCTCAAAATACCCCATCGCTCCCCCAAGTTCAGCATGCAGTGACCTTTAGTATGTTATCATGTGTGCCCAGCGCCACAATCTGTTTCAGAATTGAGTCATTTCCTGAGAAGAAAAGCTGACTTGTGCTATTAACAGTCACTCCCCGACTTCACTCCACCTGTGTCTGTGGGTCCTCGCCATTTCATACAAGGCGTTGTGACTGGCTTTTTTTTTTTTTTTTTTTGCCTGCTTATACCTAGAAGCACTCGTGTGGTAGCTCTCAAATCTCAGACTCGTGTGGTAGCTCTCGAATCTCAGTCCTTTATGGCCAAGTAGTATTCCATTGTGTGAGTGGTCACAGGCATTTGGGCTGTTGGGGAGAATGGCTCAGGCACACAGCTGGTGGGGCTAGGGATGGAGCCCAGGGCCTCAGTCGGCACGCTGAGCCCAGGCTTCTTCACCCCCTTACGTCTTTGCTTCCATTCTCACTTGGGGCGGGGGGTGTCTCTAGGAATGTGACTGTTCTTTCTTTTGCTTTTCCCCAAATAATTTTCTTTTAAACATGTTGAGCTCACATTTGAGAGGTAGAGACAGGGGGATCGCTTGAGTTCAGGGTCAACCTGGTCTACAGAGTGAATTCCAGGCTAGTGAGAGCTACGTAGTAAGGCTCCCTTTATTTTCACTTTTATAACTCTGCACATGAGGCCTTTTTCAAGCAAGCATATAATGTAATGTGAGTGTGAGCATCTCCCTCCCAATCCTCCTCCCCTCCCCCTCCTCACTCCTTTGCTCCCCAGAGAGCTTCACTTCTTCTTTCTTTCCAGCTGTGGACACAGACTTCATATTTACAAAGTCTAGGACCCACAGATGATGCTAATATCTTGTTAGGAAACCTTCTGAGACTGAGAGGAAATCGGGCTGGGAGCAGCTGAGTCCCTCCCCTGTGCCCGTCAGGCGCGGCCGTCCTCAGCTGTGGCTGCTTTTGCTTACCCACTGGCTTATCTCAGACATGTGCCAGGCCCAGCATCTCAGCTGGAAATAGTCCAGGGTTGATTTCCAAAGAGCTGAACCTTTAAGAAAGGGCTGTCAGGCCGGGGTACAGCTCAGTGCTAGAACACTTGCCTGAAATGCATGCACCAGGCCTTGGGTTGGGTCATGAGGAGTACAAAGAACAAACAAACAAAGACTTCAGTGTTACCTCTTCCTCCCAGTGTCTGTGAATATTTGGTTATTTCTTTGTCCAGTTGGATTTTGAGTTTGTGTATATCCTATTTTTGAGGTACCACCTCGTTCTGTAGCCCCAGGCTCATCTGGAAATAAGTCTTTCCACCTCTGTGTCTGGAGTCCTGGGGTAACAGGTATGTCTCCACCATGCCTGGATTTCTAGGGTGGTGGTTTCAAGTCCTTCTGAGTCTGAACAGGGCCCTGTGGCATTTGGCAGAGATGTTGCTTTCTCAGTGTTTTTGTCCCCAGGGTCTTTGGTGCCTTCTTCAAGGTGACGTGTATGTAAGA
U3_D5 115897260 115898077 818 TACAGCTACAGCTGTAGATACCCTTGGGCAGCTAACCTACCCGGGATTGCCTCTCCCAGAGAATATTGGCGCATGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGTGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTACAGAGAAACCCTGTCTCGAGAAAAAAAAAAGAGAGAGAGAGAGAATATTCTTCAAAAGGAGGGACCCTGATGAGCCCTGTAGGGACAGTGGTCTGTGTAAGGCTTGCAGCCTAGCAGCTAGTGATGTCACTGGTTCTCAGTGTGTGTGTGCTCTGCCCCACCAAAGCAGGCCTTGACCGGGGCAGAGGGCAGTCTGGGTGATGCCCCTCTGTCTATCCCACAGGTCCAAGTTGCCGCAGAATCCAAGCAGGCCAAAGGAACCCAACAATGCTGCACATGGTGCTAGAGGCTTTGAAGGCCCGGGAGGCACGCCAGGGCACATCAGTTGTAGCCATCAAGGTCTACATCCAACACAAGTACCCGACAGTGGACACCACCCGTTTCAAGTACCTGTTGAAGCAAGCTCTGGAAACTGGCGTTCGTCGAGGCCTCCTCACCAGGCCTGCTCACTCCAAGGCCAAGGGTGCCACTGGCAGCTTCAAAGTGAGCTACGGAGAAGGAGGGGGAGGCCTCAGACCCTTAGTACACGCTGCTTCTCGCCACAGCCCACATCTCCCTCCACAGCCCTCTGCTGACCCTTACTGTGCACTGTGGGGAGCCCGCACAGTATCAGGGTCTGTGTATGTGGCTTCTTGCCGGTGTCCACCA
D3_G3 115898137 115902020 3884 ATGTGGTACCTGTGGTTACTGGGGTGCCCATCTCAAGAAGTCCCAATCCTGGGGAGCTCTGCTAGGGCCTAGGGGACACTACTGGGACCTAGTTGGAGTGTGTGTGTGTGTGTGTGTGGGGCAAGTGGCTCAATAGAGCCTCAAATTACAAGCCTGAGGGAAGCTGGGAAATCAGTCCTCTCTCCTCTCAGTCTGTATTCTTGGAGATATTGAAAGAGGTGGGACTTCAGGGAGCAGGGGCAGAGTGAGGCTTGAGGCTAAGTGGGTGATAGGAAGATACAGATCTTTAGAAGTGAGCAGCAGGGCACTTCAGGGAGACAAGAGACTAACACCAGGTGAAATGCAATGAGGACCACAGGGGTAGATGTGTCTCCTCTCCTCTTCTTAACCCTGGTGTTGGGAGCATGGCCCCTTTGGTGTCCTGTGTGGGGTATAGGGATATCCAGAGCTTCTGTTCTCACCCCAACCCCATACTCTGTCTTTAGCTAGTTCCAAAGCCCAAGACAAAGAAAGCCTGTGCCCCCAAAGCCGGCAGGGGAGCTGCAGGTGCCAAGGAGACAGGCTCCAAGAAATCTGGATTGCTGAAGAAAGACCAAGTTGGCAAGGCCACGATGGAGAAAGGGCAGAAGAGGAGGGCTTACCCTTGCAAGGCAGCCACACTGGAGATGGCACCTAAGAAAGCCAAGGCGAAACCGAAAGAGGTCAGAAAGGCTCCCCTAAAACAAGACAAAGCAGCAGGGGCCCCTCTGACTGCCAATGGAGGCCAGAAGGTCAAACGCAGTGGGAGCAGGCAAGGTAGGTGTGTGCATGGGTGGCAGGGACAGGAGGTCCCCAGCAGCCACCAGGGATGCAGGATAGAAGGGGTTTCCCTCCGTAGGAAGTGGGCCTTGAGTGCTAGACCTGGATAAAGGAATGGAGAGCCTGTAGCAGCCTTGAGACAGGATCAGGAAAGGCTTCTTGGAGTAGGCATCTCCGTTGCATCTGAGCAGAAAGGCTGAAGAGCTGCCAGGGGGTGTGTCTCTCTCTGTATGGGAGGTCAGAAGAAATTCAAGGGGGTTTTGCACTAAGGAGTCTGGGCTCATCCTAGAGCAGTGAGTAGCTATGAGAGGGCTCTGGCAGGGAGCAGTGAGTGATGGTTTTAAGACCATCAAGGAAGCTGGTGCATACCCAGAATCCTGAGCACTTGGGGGTGAGAGGCAGAAAGACCAGAAGTTCAAGGCAGCATCTTTACATTTATATAGAGCTTGAAATTGGTCTGGGTCATGTGAGACTCAGTCTCAAACAACTCTACCCTCCTGAAAAAAAAGTGTGTATATATACACATACACGTACACGTACACATATTAAAGTACTACCATTGGCAGGTAGGCAACTAAGCTGATGTTCCCACGTTGTGTAAAGAGACATCTTGTCTGATTTGCAGGGGCCTGAGATTTGGGGGTAAAACAGCTGACCCAGGTCACATGGCCTTCAGGTGCCTGTTCAGGCGACAACTTGGGATCTAGGCAGACTGAGAACTCTAGAGCCTGTAGACCCTTTGTCCCTTACTACTCCCTCCATCTTACAGAAGCAAATGCCCATGGGAAAACCAAAGGTGAGAAATCGAAGCCCTTGGCCAGCAAGGTAGGTAGGTGTCTGTTGCCCACTGTGTCTCAAGTCCTTCCAGGCTTGTGTGGGGGCTCTGGGAAGGAAGCCTGTGCCTTTGTGCTTGTGAGGTAAATGCTCTGCTGGCTTGAGTTATCTCCCCACCACTCTTTTTGCTTTTTTACAGGTCCAGAATAGCGTTGCTTCCCTCGCCAAAAGGAAGATGGCAGACATGGCCCACACTGTGACAGTTGTTCAGGGGGCTGAGACAGTACAGGAGACCAAAGTGCCCACTCCTTCCCAGGACATAGGACACAAAGTACAACCCATACCTAGGGTCAGGAAGGCAAAGACCCCTGAGAACACTCAGGCCTGAGTTACTTCCCAAGACCTCCTCCAAGGCTCCCAGCAAGAAGGCTGAGGCTAGTAGCTAGGGCCAGGGCTGGGGAGATGGCGATTCTGAAGCTTTTATTGTCTAATAAGCTGTCACAATGTTTAATCATAATTTATCAATAAAGACTTTGTATTCACACACTGGTACATTGGACACTGGACTGTGAGACAGGAAGGCAGAGGCGGGTTTTGAGCAGTTGGGAGCCAGGGAGCAGTGGTTTAGTTGATGTCTTTACAAAGAGCCTCCTGCCTGCAGGAGGGATGGAGGACGTGAGGGGCCAGAGAGGGTAAGCTTCCTGAGCCGAACCATCAAGCCTCAGCCCAGAGAGGCAGACAGACTACGGCTTGGGGGAGCAGGATATTGCCTGCCAAGGAACACAGTGCTGGCTGCAACTGTAGTATGTATTCGTGTACCTGTGTCATGGTGTGTGTATGGAGGTCAGACTACAATTTGAAGGGGTCCTCTCCTTCCACTTTGTGAATCCCGATGGAATTTGGGGTATCAAGCACCTTTGTCCAGGGAACCATCTTGCCAGCCCTTGGTGCAATTTTTGATAGGGGAGGGCCGCTGAGCAGGGACCCCAAGTCTGGATGGAATCGTATTCAGCAAGAGAAGGAAAGGGCACAAAGGCCCTGTATTGGTTACTGTCCATGTTGCTGTGATAAAATCCCGGACAGGAGAGAGTTAAGCATGGGAAGGGTTTACTTTAGCTTGTGGTCCATCACGGCAGAAAAGGCCCAGCAGCGGGAGTGCAAGCTCAACTCTTGCTCCTCTTGACTCCATGTGGGACTCCAGCCCACAAGATGGTTCCACCAAAACTCTGGTCTTCCCACATAAACATCTCTGGAAGGAAATGGCCTCATAGATATGACTGGTGGGGTGTCCCTAGGCAATTCCAAATCTGGTCAAGTTGGTAATCCACCAGTGGCCCTAGGGCAGGGATACGAGTGAGACAAGTCATGGGGCCAAATTCTGCACTGCTTTAAAATCATCAGAGACACTTTTCCATGGATAGGTGGAGTTAGTTCCCTCACCCTGAGTCTGAGCTCTGCATTGCTTGACCAGAGGGCAGTGGCAATGTCAGGACAGATCTGAGACTTCAGAGGAGGCAGGGTGGCTCAGTGGGTAAAGTGTTGGCTACATAAACATGAGGGCTAGAGTTCAAATCCCCACCACTCGCATAAAAAGCTGGGTGTGGTAGTGCATGTGTATAAATAACCCCAGTGTCAGGTGGTGGAGGCAGGAGGATCAACCCCCAGGGCTCACTACCCAGATAGCCTAGGCTAAAACAGCAAACTACGGGTGAAAGAGTCAAAGCTCTCAAAAAAAAAAGGTGGTCCAATGGTTATAGCCAAAGCACTGGCTGTGTATGAGACCAGAGTTTGTCCCCCCAGAACCACATGAGTGCCAAGTGAGTGTTGTGCACCCCATTAATTTCAGCCTCAGTAGGTGGAGATTGTGTCCTGGCTTGTAGGACTGTTTTTAGCTTTGGTCAAGCTGGAGTCACATGAGGAGAAAACTCAGTTGAGAGAACATCCCTACCAGATTTACCTGTGGGGAGTTCTCTTGACTGATTAATATGGGAGGGCGGGCCCAGGTCTCTGTGGACAGCACTCCTGGGCTAGTGCTCCTAGGTTGTATAAGAAAGCAGCCTGAGCAAGGCGATAAGCAGGTTCCCTCCATGGCTTCTGCTTCAGTTCCTGCCTTGAGTTCCTGTCTTGACTTCCTTCAGTGATAAACTGTAAAGCTGTGAGCTGAAATACATCCTTTCTCCCCAAGTTGCTTTTGGTCATGGTGTTTCATCACAACAATAGAAACCTTAACTTCAATGGGACCCTTAGAGCAGGCTGGTTAAAAAGACCACTATCCGAAGCTCTGCATTTGATTAAGACTACCTTGGAGAAGC
U5_15 115882160 115897208 15049 GCTCATCGTGCTGGGCTTCCCCATCAACTTCCTCACGCTCTACGTCACCGTACAGCACAAGAAGCTGCGCACACCCCTCAACTACATCCTGCTCAACTTGGCCGTGGCTGACCTCTTCATGGTCTTCGGAGGATTCACCACCACCCTCTACACATCACTCCATGGCTACTTCGTCTTTGGGCCCACAGGCTGTAATCTCGAGGGCTTCTTTGCCACACTTGGAGGTATGAGCAGAGAGACTGGGGCGGGGGGGTGTAGCATGGGAGCCAAGGGGCCACGAAAGGGCCTGGGAGGGTCTGCAGCTTACTTGAGTCTCTTTAATTGGTCTCATCTAAAGGCCCAGCTTATTCATTGGCAAACACTGTGACCCTGAGCTAGGCTGCTGTTGAGAGCAGGCACGGAACATTCATCTATCTCATCTTGAGCAATGCAAGAAACATGGGTTCAGAGAGGCCAAGGACTCACCGAGGAGTCACAGAGTGTGGGGGTGTCCTCTGAGGCAGCTGAGCTGGGGCACACACAGACTGAGCACCAGGAGTGAGCTCTAGCTTTTTTTTTTCTATGTGTCTTTTCTAAAAGACACATAGGTTTAGGACTGTCCCTGGTCCAGGTAAGAACTGGTTCAGTAAACTTGTACATCTCACTGCCTGGCCAGCCCTGTCAGCTTCCACCAGAGTGCGTGCACTACACACCCGGCATCTCAAAGGATTCATTCCTATCTTTCCTATCTTTGGAGTGAGGCACAGTCTCACGTAGTCCAGTCCAGACTGGCCTTAAATTCTGCAGCTGAGGATGTACTTAAACTTGTCATCCTCCTGCCCCAGCCTCTCAAGTGCTGTGATCACAGGCACGGACCACTATGCTACGCCAGGTGTTTCCAAACATTTTCTCTCCCTTAACTGGAAGGTCAATGAGGCTCTTTCGAGAAGCAACAGAGCCTGTTTAGCTGAGAAAACTGAGGCAGGGAGCAGGCAAAAATCACATCTAGAGATATGGGAGAGCCAGGACCAGAGCCAGGGTCTCCGGGCTGGGACCTAGAGATGTTTCCAGTGGATACAGGAGGAAACAGAAGTGGGTGTAGCAAAGCCCAAAGCCAGGGTGATGGGTGGGTCGAGCTTGCTTATCTCCCCGTGTCCAGGGTACTGCCTTGGTAGCACTGTTGGGCATCTCTGTTACCTTCTGTGGTCACACACACACACACACACACACACACACACACACACACACATACACACACACACACCCAGGTTCTGGATAGAAGCTGGGGTACCATGCCGGTGAGCTTGTCTCTGTGGGGTCAGACCCAGGCCACATCTACCATCCAGGATTCTTGTTGGTAGCACTCCTGTTATTCAGAAGTTTGTTACGTGGTCCTTCCCCACACTGGGCTTCTGAGGCTGACATATGGACTAATGTCTGGAGCCCCCTCTTTACCCATCTTCTTCCCCTGCTAAAACACTAGCCAGGGTGTGGCCCTAAGCCCCAGCTCCAGGCACTCCGAGGCAGTCTCTCATGAGCCTAAAGCTCTAACCAAACAGAAGAGCTTCTGTTTTGGCACACGGGTTCTTCACCCCATCCCTTTCTCCTCGCCAGCCCAAACTCACTGCAGTCGCTAAGGCTTGGATCAAGCCTCAAACCAGAAGCTTGCATCCTAGCCTGCTCTCTCTGAGGTGAGGTTAGAGCTGGAGGACTGACGGCTACTAACTGCCTTACAGGTGAAATCGCCCTGTGGTCCCTGGTGGTCCTGGCCATTGAGCGCTACGTGGTGGTCTGCAAGCCGATGAGCAACTTCCGCTTCGGGGAGAATCACGCTATCATGGGTGTGGTCTTCACCTGGATCATGGCGTTGGCCTGTGCTGCTCCCCCACTCGTTGGCTGGTCCAGGTAATGGCACTGAGTATCGGGTCTGGCAAGGTCTTTGTGGGATTCCCTTTGAGGACACAGAGCCCTCGGATTGGTTCCAGGCATAATGTAACATGGTATTGCCCCCCGAAAACCATCCTGGTGACTTTCCCAGGCTAAGGTCTAAGGTAGGGGAGAAGAGAGGGACTGAATGGTCCAATCAGTCTTATTCCATGTCTGAGACCCATAACAAGGAGAACCCTGGACATTCCAACCCTTCACCTTGGCCGAGTCCCTAATCCTCGGCTAAGCCAAGGCCAAACCACAATCCTCTTTGGTTGAGTTCTGGCCGTGGGCCTCTCTCTCTCTTCCTCTCTCTCTCTCACTCACCTTGGACCTTAGCCCCTGGAGAGGCTGAACCTTCCCAAAATGCATGGTGACATTGTAGCCCCAGGAACTGGGTCCCATCCAGCCTCCAGGCCACCATATCTAAATGAGACAAGAGAAGGTTGGGACAGTGGTTTGGACACCTAGACAGGCTATGAGGTACACAGAGCCTCAGAGACTCTCCATTCTCTGTCCTCATGTCCTCCCCACCCCGGGAGCCCACCCAGTGGCCCTCACTGAGTCAGAGCCCCTCACTCCTCACTGGCCTCTTTCCTCATCCTCACCCACCTGGCTTGGCAGGCCTCTACAGACACACTCAGTGGACACTTGGGTTTCTGAGTGTGGCCCAGTGTCACCGTCCTCAGATATCATCACAACATCCTTGTTTCTAGAAGCTGCACACAGCCCTGATGCCAGCAGCGAGCCCACCTTTACTGTAGAGAGCATTGCCGTTACTAGGAGATCCCATGCACAAAGTGCAGCATTCCCAGGGAAGGCCTCAGAGAATGCTCCTCTCTCCAGCATTCTCTGCCTACTCCCTTAACCACCGAAGGCAGGGCAGCAGGCTAGTGGAGCAGAGCTGCGTGGTCAAGTGGCAGGGAGCTTAAGAATCGTCCAAGGGCGGAGACCAGTAAGTCTCATTAGGTGATGGGGCCAGCAGGTAAAAGCCATTCATGCTTATGTCCAGCTGGGCGTGTGTTCTCTTCCTGTTTTATCATCCCTTGCGCTGACCATCAGGTACATCCCTGAGGGCATGCAATGTTCATGCGGGATTGACTACTACACACTCAAGCCTGAGGTCAACAACGAATCCTTTGTCATCTACATGTTCGTGGTCCACTTCACCATTCCTATGATCGTCATCTTCTTCTGCTATGGGCAGCTGGTCTTCACAGTCAAGGAGGTATGAGCAGGGGGGCCGCCCCAGCCTCGTGCCGGGTGGGTGGGCAGAGCCAGGTGGGCAGAGCTGGGTGCCAGGGTTCGTACAGACGCCATGGGCTAGACACAGGTCTGTGTCCCTGCAGGCGGCTGCCCAGCAGCAGGAGTCAGCCACCACTCAGAAGGCAGAGAAGGAAGTCACCCGCATGGTTATCATCATGGTCATCTTCTTCCTGATCTGCTGGCTTCCCTACGCCAGTGTGGCCTTCTACATCTTCACCCACCAGGGCTCCAACTTCGGCCCCATCTTCATGACTCTGCCAGCTTTCTTTGCTAAGAGCTCTTCCATCTATAACCCGGTCATCTACATCATGTTGAACAAGCAGGTGCCTGGGCTGAGGCAGGGCGTGTGGAGAGTGAGGGAGAGAAGGGGAGGGGGAGAATGGGACAGGGGAGGCATTGCACTCAGACTGCTGGCAGCCCGTGAGCTCTCAGGCTGGCGAACCCCCAAGCTCTTGGAGTAAGTCTTCCGGGTGACCTGCCGTCCACTCCTGTGAGATACAGCCGACCAAATGGTTCTGGAAGTCTGCTTTAGAAGTAGGCTAGAAGTCAGGTGCAATAGTGCAAATTTGTAATCTTAATTCTGAGGCAGGAGGATCTTGAGCTCCAGGTCAGCCTGGGCTAAACAGTGGATGTGTGTCCCCCTCTCCCCAAAGCAGGCTGTGAAGCACACTGCTATATCCCAGCATTCAGGAGACTGAAGTAGGTCGATTAGAAATTCAAAGCTATCTTTGGCCATACAGTGATTTGGGAGCCAACCTGGGCTACAATGAGAATCTGTCTCAAGAAGCAACCAGAAATATCAAGTAGTTCCAAGTCTGGGAATAGAATGGTCTCACTGTAAAGCTGAAGAAATTGCTAGAAATGCTACTTGTAGGAAGTCAGGGATTACACCACTGTCAAACGCCACAGTCTCTCTGCAGAAAAGGATGTGGTCTCTGGAACCTAGACTCTGGGTTCAAGTTCTAGTTCTGTCCCATTTAAGCTGTGTGACCTTGGGTGAGTCAGATATCTCCCCATGTGGACTTCGGTTTTCCTATTTATAGGAAGGGCCAAATCATAAACTCTCAAATCAATTGAGATCACTCACGGAAGGCCCTACGCATCCACAGGACACATGGAATGCCACTTGACAATACCCCTTGTGCTATCTGTGTACCTGGCGTCTCTGGTTGAAGGGCAACATACATTTAGCAAGCTCCCCCAGGGAGCCAGGCTTAGTGAGGGGACATGCTGGAGGTGAGGCTGAAGCTGGGCAGGTGGTAGGGCCTGTCCTGACTGGAGCCTCTTGCCTTCCAGTTCCGGAACTGTATGCTCACCACGCTGTGCTGCGGCAAGAATCCACTGGGAGATGACGACGCCTCTGCCACCGCTTCCAAGACGGAGACCAGCCAGGTGGCTCCAGCCTAAGCCTGGCCAGAGACTGTGGCTGAAAGTAGGAGTCTCCTGTCCCCACACACCCCAGCCACAGCCCCCACCAGGAGCAGCACCCGTTGGGATGAGGTCATGCAGGCTCCCTCAGTGTTCTTTTCTTTGTTTTTAATGAATTCATGAAAGCAAAATGAGGCTCCCCACTCAATGGGGACAGCTTGACAAAGGGCATCCATCCACCAAGACCATCCTCAACCTGGAGTCCCCAATTCCCGGGGGGCCAGCGGGATCTGTACCCCTCCCTCAGCTTGTCTATCAGGAACATGACAAGTGTCCCGGCTTAGGGCTAAATGTCTAGGACAGAATGGAACACATAGTAGCTGATTAATAAATGCTAGCTGGATGAAGGGAGGAATGAGTGACTGACTGAGTGGATATATGAGTGAAGGGATTAATGGAAGGGAACATGGATGTCCTCAGGTGCCCAACCTGGCAGATCCAGTCATGTCTGGCTGGAATCTATAAGCAGTTTTACATACCTGCCCTGGTTTTCTCTGCCCCCACCCCCACCCCAGTTGGATCTCCCAAATCCAGGGCCCTGATAGAATATGGCTGCTTCAAAGACAGAGAGATGAGGGGAGGGAGGGGGGAGGGAGAGAGGGAGGGAGGGAGACACAGAGAGGGAATATGTGTGATGCGTGTGTATGTGTGTATGTGTGTGTGTAAACACTTTGTATATAAAGAGTACAGCTGGTAGTTATGTTACAAGTAACACCGACTAATATAATTAATTAACCATCCTAATGGTCTCTGCTTGTTAGTGACTGCTTGGGAATTAGGCAGGGCCCAAGCACTCAGATAAGGTATTTCCCTCAGCCTCAGTAGGCTTTTGCAAATGACCCAGGCCTTCAGGCCTGTGCAGGGCTAGAGCTGGATTACAGAGATAAATGACAGTGACAGCAACGTGAGCTGCAGCCCTTAGGACTGAGAAAGCATCGAGACCAGGGGTCTCCGGCAAGGCCTAGGTCCTCCCTTCAGTATGGAAACCTTGCCTCATGTCTCTCAGCCTCCTTGGCCTGTGGAGATCCAGCCCTTCCTCTTGGCTTCTGGATACATTTGCTCTTCTACACCAGCAACCAAGTGGCAACAGTTCCAGGCCAGTATGGAGTTTTAGAAGCCATGCCAATATGCCCACCTTCAGGGAGCAGCTGAGTCCTTGATGCCACCCTTGTTCTGAAGAGTTCAGAAACACAGTGCAAGACATGACCAGGCCTCATCCTTAGGATGCTCATGGATCCAGTTCTTAGCTCCCTTGTTGGATATGCTGTTTTCCTTGGCCTTTGGTCTTTTCTTTATCCCAGAGGGTTTTGGCTTTAAGGCCAACAGGAACTATGGGGTACCAGAATTGAGCAGCCTCAGTCTGCATCCCTCCTCTATAGAACCACAGCTGGGCCCTCAGCAGGCCCAACTCTGCATGGGGACAGAGGCATTAAAAGCTCAGCTCCTACACTTGGTGGCAGTGGTGGTCTGTTGCTCTCAAGCTCTTTCAAAATGGATGGAAACTGGGACGCTTCCCTGACCCCTGGTTATGAAAGACTAGACTGTGTGGGGACAAACAGTCCAGAGTCCCGGGGAATGTGATAGAGCAGCTCCATCATTTTTAGAAACCCAATTTGAGGCAGTATAGAGAGATGGTGACCTCTATAAGCCTCTGTATCTGCAAAGAGGAGCTTAGACCTGCCCTTGAGGGGATTATATGAGATTTAAGGGACTTATGTGGCCAGCCTACTTCCTGGCATGCTGAAGACATTGGCACACTCTGGTATTCTAGACCTTGGCTCAGAGCTGCCTTTACTAGGATACTGTCACTTAGCAAAAGAATGGGATGGAGCCTCAGATGTGGAGTGACACCATCTTCCAAGAAGGAAAGGGTGCCAGGGTCTGGGATGAAAGCCCTTTGGTGCTATGTTGGGCAAGGGCGAGTGCCAGCAAGGGGTTATTTGCTTGCTCTCTCCATCAGTGATGAGGTTCCATTTGGTCACAAGAAATTCACCCCAATTGCTGAAACAGAGGCTGACTATTGGCTTATAGGCATGAAACCCCACTCCCCTCCACTTCAGGCTGGCTAGATTAAAAGCTCAGACCTGTGACCCCTTGTGTCTCTCTTCTGTGTTGACTTCACTCACAGGCCTAACCTCAGAGGTGTCTGTAGCAGTCCCAGGGCCAGACACCCTTCCAGAAAGGGGCTTTCCTTCCCTTTATAATCGTGGTGACAGGTCAGATTCTTATTGGGCAGACCTAAGTTACCTGATTATCCTTAAGCCAATCATCATGTCTAAGACAGAGATTCATTTGATTGGCCAGGGCTGAGTCACTTGTTTACCTCTAAGTCAATCAACAGGCCTGAGATGGAGAGTCATCTTCTGATTAGCTAGACCTGGGTCACCTGATCACCTCTAAGCCAACCACTATGTCTGATATAGGGCTCCCTCATTTGTAATGTGTTCCCACACTACAAGATAAAGTTGACTCCACATAGTTAAGGAGCCTGTAGGATGTGTTCCCCAAAAGCAAACAGGGGAACAGGATCAGGACCAGCCCTGAGGGGTGGGGACTGGGATGTGCTAGACAAGGCTCCGGGGTGTCATGGATGAAGGGGATTCCATGGCTGCCACACCTCTGGTTGTCTCTCATGCTTTATGGGCCAGGAGTGCCTGTTGGAAGGCTACTTTAGCTGCCTGGCCTTGACCTCCCTCACTCCTCCATGCCCAGTTCACTTGTCCTGTGGGGTGTCATCTTTACTCCTGCCTTCTCCTGCCCGGGAAAAATAACCCTTTAGAACCAGAATGCTCTTGTCAGCCTCCCTCGACAGTCACTGTGAGTGTAGACAGAAGAGCTCCTGCCATGAGCTGGGACCTGGAGTCCCCACAGGGTCACCTGCCAGGCTCCAAGCAAGCAGCCTTTGTGGAACATACAACACACTCAGCCAATCACAGGTCAGACAGGGCTGTCACCCCAATAAAGAGACACCTGGGTTACCAGGTAACCTGAGGTGCCCCAGAGAAACACAGTGCCAGTCCCAGGGTAAGTCCCTAGACTACACAGGCCACAGCTCTGAGCTGAAAGTGAGAGACACCTTAGCTGGTGGAGGCTTTATTTCTGTATTTGTTTCCACCCACGGTCACTTTACCTCTGAGAAACATTGAGGGTGACCCCAGATATATAGGGAATTAAATAGGAACACAAGTCAAATATCTACTGATAATCATAAAGAAACCTAATGTGAAGTAAGTCTTTGATATATATATGCAATTTTACAATTGATTAAAGATGAAAATAAATTTGCATTCTAATGAGATGGATCTGCTTGCTTTTTCACAAGGAATGATCTTAGCTGAATATTTAATGATGTAGGACACAGCATATACGACGTTCTTTAGAGCTGATATTTTGATTTTATAATCATTGATGCTGAGAATGCCTCTTCGCATAAATACATAGTATAACATTAAGAAGTATGTCTAGGCCACTTCAGAAATTGCTGGAAATTGTTTCACTATCCTAATCCAAAATTCACTGAACAGTGTGTGTGTGTGGTATGTGTGTATGCATGCATGTGTGCATATGCCTGTGTGTCCATGTATATATAGAGGTGCTACCACCTGTGTATATGAGAGTGGAGAAGTTGAGGTTACAGATGGGGTCACACCCAGCTTTTCCATGAGTGCTGGGGCCCCAGACTCAGGTCCTCATCCTTCACAGCAAGTGACCCTAATTAAGAGATCACCCCAGCTCCGAAGCAAATGACTTCCATCTGAAACTCAAGTCAGCCATTCAAATAGCAGTTTTAAAGATCAAACATTCTAACACAATGCAACCTCAAAATATACTAATTTAATCCTTACATGCCGAGGAGTGATGGTAAGAAAATGTTGAAAGTCTTTGTTTTCTACAACTCAACTCTTAATTGTTCCAATAACACAGAAAACTTTGTATGTACAACCAAATCAGCCAATCAAACCAAATACTGTAGTTTCCAACATCCAACAGTCTGGAATCTGAGCTGTCATTCAGACAGTTTGTTGGCACTGTGTGCTATGAAGGCATGGGCAGTCCAAAGTGCACAAGTATGAGCAGAGCCAAGGCTTTGTGGAGTCACATGATTCAACACCGGTGAGCACTGCCCCAAACACCTCAGATCCATTGTTTGATTTTGAGTTGATGTTAAAAAAAAGTATATTACATTTTGTTTATTGCAACTATCAGAGGAGAGGGAACACAGGCATGCCACAGTGTGAATGTGAACATCAGAGGGCAGCTTTTGGAAGTCAGCTCCCTTCTGCCACAGGGGTCCTGGGGATGGAACTTGGGTCATCCGGTTGCCAGCAAATGCCTTTCCCCACTGAGCCATCTTGCCTACCCTTGAATTACTTCTTGATCTTTGTATGTTCAGAAACTTACTGTTCCCAAAGTTTCTATCACCTCTACCCCCCTCCCCCCAATTCAGGGGTAGTGCCCTGTGTGGACCTGTGACCTAGAACCTAAGAAATAGTATATGAAGGATCGAGAAGACATCAGTATGAATGGCACTTCAGGCAGCAGCTGTTCAATGCATGAGGCTTCCCTGCCCAGGGATGAGGCCAGGGTTCAGCAGCAGCCCCGGTGGGGGAGGGGCGGGTGCAGAGATGAGCTTGATGACGACCCAGGAAAGGAGGAAGAGGGGCCCGAGCGAGGGGGGGGGGGGCGGGCGGGGGGGTTGGAGGCGAGGCTGGGGCGGGGCGGAGGAAGTTCTAAACTGAGCGCCTGACCCCATGCCACTATCACCTTCTCCTCCTGATGGCTTGGAAGCATACAACTCTGGGCTGTGAAATTCAGAGACCAGAAGGGCCCAGCACTTTCCTCTGCTTTCCTTGTTGGCTGGAATGTGATCGTAGTGACTGGCGCTGCAGCAGTCTTGTTGGATCATGAAATGGGCCCTAAGTCAAAGAAGAGACAGGGTCTGGCTCCACTAGGGCTACCTCGACAGTTGGGTCCTCACCAGCCAGGGCGACTTAAAGAAGTTCTGAAGGGGAGGCTGGCGACAAGAGAGAATGGCTGTTTGGGGATTACCACACTTATCCTTAGTACAGAGGACTTCCAGGATGATAGGGTCTGAGTGTGGGGTGAGCCAAACTTCTGGACTTCAGCCTCTTCATGTGTAAAGTGACGAAGATGCTGATCTATACAATTGTATAATGTCAATTATACGGTTTATACAGACATGCCAGTGGCTGCAGTTTCACTCCACCCTGATGCACTGATTAGGAAATCGATGTCCCAATGTCCTGATGGCACAGGATCCTCTGACCATCTCAGAGTTCGACATATTTATCTCTGAAAATGGAGCATGTCCTAGAGTGGAAGGAGAAGGTTCCAGAAGGAAGGCAGCATCCCCCCACCCCCGCCTGGTCCCACAGAGAAGTCAGGAGGCTGGCAGAGGGCACGCTTCTGCCAGCACCTCCCTAACTTGCTATCATCTCCCTGTCAAGTGGCAAACCCCTTGGAGAATTTTTAGATCTCTGAGTTTTTCCCAAACTCCCTTCCCAACACAAAACTCCATCCTGGCACAGCCTGGGAGCAGGGTTTCAGCAAAGCTCGTGGGAATGTTTACACCACAGAGTCTCTGCGTTTCTCAGTTCTTGGAAATGCCCCACAAATGGATCAAAATTGCGGCCCCCCGTGTGTGCCAGCTCCCGTTTGCCTCTTTGCCTCCCGGAGCGCCTTCTCCGTCTAGCACTGGGCATTTGCAGGTCTCTGGTCATTCCTTGGAGCCATAGCACCGCAATAAGCCTGGAAGGCCTACTGTGTGCTAGTGTTGGGCTGAGTGCCAGAGACAGCCTTGATCCACCACACAGCGCAAGAGTTAGGGACCTGTAAGGCAGTGCTGTGGCATTCCTGAGCAAACCTTACCTACATGAGAGAGATAGGAACAGAAGGCTTTCTAGGCCTGATGTAGTAGCAATCTTGTGCTGTGTGACAAACATTCCTAGAATTTACAGTTTAAAACAACAAGCCACATGTCATTTCTCCCAGCTCCAGGGATGTTGGATGGTCTTCTAGCTAGGGGATGCTGCTGACTGGGATCCAGCCTGGACCATCTCTGCTAGGCTGCTTCAAGCATCCATGGTCAAGTGAAGGACTAGCTGATGACCAGTTGGTTGCCTGGATGTTGGCTGTGTAACGAGTGACTGGACCACGGGTCTCAGCATCCAACACACTAGCCAGGGCTTGCTCCCATGACAATGGGATCACGATAGCACCCTTCCTGTGGTAGCTCACAGCACAGGGCTACCACAGATATAAGACTGAGATCAACGAAGCCTCATTGAATGTGTGTATAGATATGGGCAGAGATAGGAACTCTGGATCATTGCACATTAATTTGACACAGAAGCACAGAAGTGTGACATCCTAGTTTTAGCTATAGTTCATTCAAAGGTCACATGGTGTGAGTTTTTTTTTTTAGTTTATTATTGAAGGTTGAACCCAGAATCTCAGAGATGCTAAGCCCTCATTCAACCACTGAGCCCCAACCCCGAGTCCTACCTGCAGTGTGGTTGAATTGTGGAATTCAAGGGAGAGGAAAGCGCCATGAGCCTGATGGAAAATCTCAAACTTTTCCTCAAAGGAAGTAGGGACCCATGGGAGATTCTAGAAATGGAGTGAGGTCTGTTTCACTGCAAAGAACTTTGAGGGCCACAGCCTTTCTCAAACCAGACTTATGATGGATGGTGAGTTCTCTGGGAAAATTCCAGAAAATAGAGGGATCACACACACAGAGGGGAGAAGCTTGCAAGTAAGGCATCCTCAACCTTCACCTTTCCCAAAGCAGCTGCTGCTACCACACTGGTGTGAGTCCTGGTAGACCTGAGCTCGAGTCAGGGCCCTGCAAGGCCGTGCATTACAGTAATGAGCAGACCTTATCTACAGGAGGGAGCTGAAGGCCTTCTTCAGCGCTGGCCTCCCCATTCTCGCAGCTGTGTCCTATCTCCCTGTCAACAGGCAACAATTGACTGAGCCTGTTCCTATCCTGACAAGTTTTTAGTTTGTCTTGAATTTCTGATGATACTGGGGCAAAGTTCCCATTTGTTAGTCCCCCTGTTTTTCCCCAGGGAGGGATGGCTGAGCCGTGAAGCAAGCTCATCTTTGCTCCATAAAATGTTGAGTTGCCTCTAAGGTGGCTGGCCTGTCAGCTCTCCCAGCTTCTGTGTGATTCCCGTACAGTCACTATGAGAAATGCTCATCTCATATTTCATAAGCAATATTGACATTCAGCCAGGAGAGAGATTCAGCTTGTTGTAATCCATCCTGGTTTTGTTTCCTTTTGGTTTGACTCTTTTTTTGAGTCAGGGGCTTGCAGTCTAGGCTAGCCCCAAACGCCCTAGCCTCCTGCCTCAGTTTCCCCAGTGTGGGAACAAGAACAAGCCACAGTCATATATGCTGTAGCATGTTTTACAGCGATGGAGTCATTTACTCATGCCCATGTGGCTTCACTGAGAAGTCACTTCTGTCTTTCATCTTGATGACTCAACCCTTCCTAGGAAACAGGCAGAATGACCAGGCTCGGGAGATCCCTTCCCAAGTCATAGCCCTGTTGGGGGTGGGGGGGTGGCAGCTGGCTGGTTCTCTCACCCTAGCCAGTTCCAGTGTTGGGTAGTATGATGGAAAACAGTGACTTAGGTCTGTCCCAGTGTGTCTTGGAATGCCAAGGACACTGAAGACAGTGACCATCAGCCAAGGTCACATAAAGACCCTGACACACCTTCCCCTACATCTCAAACTGCAGTAAAATCTAAAACATCAAAATAACACAGAAGGAAGAGATCTGAGAGCCAGCCTGGCTCGCTCCGGAGAGGCCACTGCTTCCAAACACTCCCTCCCTTGGGCACAATTGCTGCTTTATGGAAAACTGAGGCTAGCAGTAGTCTGGATCAGGGTAGACAGATTCCTACCCTTGTGTGATTAGGGGGCCTTTGTGAGCAACCCCCCAACTCACAGTGTTAGATGTCAGCTCGTCAGGTAGGTAGGACACAGTGACACTGCCTCCTTACTTCTTCCCGTGGCCTCAGGTCCCACAGGGTAAGGTGGATGCTGTGTTCAGAGTGAGTCTTACCCTCGAGGCCTGATTGGCAGCTTTCGGCTGCTCAGGTGCTAGGCATGTTGGTCATTGGTTAAGTTCTGTTGGGAGGTATTTAAGGCAGAAGGTCTGGAGCTAACTGAGCTAACTGAGCGAGCACTGGCCTGGCCTGCTGCTGCCTGTGCCATGGCTCCTGGGAGTGTCTCCAGTGTTTCTTCCTCCTCTTTTCCCTCCAGGGACACATCCCCTTCTGGATCATGTGGGCTCCCTGGAGCTGACAAGCCAGGTAAGCAAGGGAGTGGAATTCTGAGTTTAGGCCAAGGTCCCTGGAAACCGGGAGACAGCCCGGAGACAGCCGCTGAGAAGGTGCATGCGGCTTAAGCCTGGTTCCCACCGCTCGCTCGGATTGGAAGAGGAGACCTAGTCCTGGTGGGGAATGAAGCTGAATGGTCCCATTTCCTGGTCTGGGACTCACAGTGAAGACTTCAGTCAGGTGCAGGCTGCCTCGGCCCTGCTACAGGACTCCCTTAGGCCCGTGGCCTGTTGTTTCATGTTTCTTTCTTTTTTCTTTTACACGCATTGGTGTTTTGCCTGCACATATGTCTGTGTCGGGGTACTGGACCCAATGGAACTAGAATTTCAGAGAACTGGAGTTCAGACAGCTGTGAACTGCCATGGGAATTGAACCCTGGTCCTCTGGAAAAGCACCCAGTGCTCTTAACCACTGAGCCATCTCTCCAGCCGATGGCTTTTTCTTTCTACTTCTAAACTTAAATTTTGAGTCTGAATTTTGCAAATAATTCTGACAGCATCACCCTGAATTTAAGTCACGCCGACAGGTTCTCCCATTTCTCCCCCCCAAATCCTCCTTTTTCTTGCTGCTTTTCAAAAGTAACAGCCCATCTCAAAATACCCCATCGCTCCCCCAAGTTCAGCATGCAGTGACCTTTAGTATGTTATCATGTGTGCCCAGCGCCACAATCTGTTTCAGAATTGAGTCATTTCCTGAGAAGAAAAGCTGACTTGTGCTATTAACAGTCACTCCCCGACTTCACTCCACCTGTGTCTGTGGGTCCTCGCCATTTCATACAAGGCGTTGTGACTGGCTTTTTTTTTTTTTTTTTTTGCCTGCTTATACCTAGAAGCACTCGTGTGGTAGCTCTCAAATCTCAGACTCGTGTGGTAGCTCTCGAATCTCAGTCCTTTATGGCCAAGTAGTATTCCATTGTGTGAGTGGTCACAGGCATTTGGGCTGTTGGGGAGAATGGCTCAGGCACACAGCTGGTGGGGCTAGGGATGGAGCCCAGGGCCTCAGTCGGCACGCTGAGCCCAGGCTTCTTCACCCCCTTACGTCTTTGCTTCCATTCTCACTTGGGGCGGGGGGTGTCTCTAGGAATGTGACTGTTCTTTCTTTTGCTTTTCCCCAAATAATTTTCTTTTAAACATGTTGAGCTCACATTTGAGAGGTAGAGACAGGGGGATCGCTTGAGTTCAGGGTCAACCTGGTCTACAGAGTGAATTCCAGGCTAGTGAGAGCTACGTAGTAAGGCTCCCTTTATTTTCACTTTTATAACTCTGCACATGAGGCCTTTTTCAAGCAAGCATATAATGTAATGTGAGTGTGAGCATCTCCCTCCCAATCCTCCTCCCCTCCCCCTCCTCACTCCTTTGCTCCCCAGAGAGCTTCACTTCTTCTTTCTTTCCAGCTGTGGACACAGACTTCATATTTACAAAGTCTAGGACCCACAGATGATGCTAATATCTTGTTAGGAAACCTTCTGAGACTGAGAGGAAATCGGGCTGGGAGCAGCTGAGTCCCTCCCCTGTGCCCGTCAGGCGCGGCCGTCCTCAGCTGTGGCTGCTTTTGCTTACCCACTGGCTTATCTCAGACATGTGCCAGGCCCAGCATCTCAGCTGGAAATAGTCCAGGGTTGATTTCCAAAGAGCTGAACCTTTAAGAAAGGGCTGTCAGGCCGGGGTACAGCTCAGTGCTAGAACACTTGCCTGAAATGCATGCACCAGGCCTTGGGTTGGGTCATGAGGAGTACAAAGAACAAACAAACAAAGACTTCAGTGTTACCTCTTCCTCCCAGTGTCTGTGAATATTTGGTTATTTCTTTGTCCAGTTGGATTTTGAGTTTGTGTATATCCTATTTTTGAGGTACCACCTCGTTCTGTAGCCCCAGGCTCATCTGGAAATAAGTCTTTCCACCTCTGTGTCTGGAGTCCTGGGGTAACAGGTATGTCTCCACCATGCCTGGATTTCTAGGGTGGTGGTTTCAAGTCCTTCTGAGTCTGAACAGGGCCCTGTGGCATTTGGCAGAGATGTTGCTTTCTCAGTGTTTTTGTCCCCAGGGTCTTTGGTGCCTTCTTCAAGGTGACGTGTATGTAAGA
D3_15 115898137 115913187 15051 ATGTGGTACCTGTGGTTACTGGGGTGCCCATCTCAAGAAGTCCCAATCCTGGGGAGCTCTGCTAGGGCCTAGGGGACACTACTGGGACCTAGTTGGAGTGTGTGTGTGTGTGTGTGTGGGGCAAGTGGCTCAATAGAGCCTCAAATTACAAGCCTGAGGGAAGCTGGGAAATCAGTCCTCTCTCCTCTCAGTCTGTATTCTTGGAGATATTGAAAGAGGTGGGACTTCAGGGAGCAGGGGCAGAGTGAGGCTTGAGGCTAAGTGGGTGATAGGAAGATACAGATCTTTAGAAGTGAGCAGCAGGGCACTTCAGGGAGACAAGAGACTAACACCAGGTGAAATGCAATGAGGACCACAGGGGTAGATGTGTCTCCTCTCCTCTTCTTAACCCTGGTGTTGGGAGCATGGCCCCTTTGGTGTCCTGTGTGGGGTATAGGGATATCCAGAGCTTCTGTTCTCACCCCAACCCCATACTCTGTCTTTAGCTAGTTCCAAAGCCCAAGACAAAGAAAGCCTGTGCCCCCAAAGCCGGCAGGGGAGCTGCAGGTGCCAAGGAGACAGGCTCCAAGAAATCTGGATTGCTGAAGAAAGACCAAGTTGGCAAGGCCACGATGGAGAAAGGGCAGAAGAGGAGGGCTTACCCTTGCAAGGCAGCCACACTGGAGATGGCACCTAAGAAAGCCAAGGCGAAACCGAAAGAGGTCAGAAAGGCTCCCCTAAAACAAGACAAAGCAGCAGGGGCCCCTCTGACTGCCAATGGAGGCCAGAAGGTCAAACGCAGTGGGAGCAGGCAAGGTAGGTGTGTGCATGGGTGGCAGGGACAGGAGGTCCCCAGCAGCCACCAGGGATGCAGGATAGAAGGGGTTTCCCTCCGTAGGAAGTGGGCCTTGAGTGCTAGACCTGGATAAAGGAATGGAGAGCCTGTAGCAGCCTTGAGACAGGATCAGGAAAGGCTTCTTGGAGTAGGCATCTCCGTTGCATCTGAGCAGAAAGGCTGAAGAGCTGCCAGGGGGTGTGTCTCTCTCTGTATGGGAGGTCAGAAGAAATTCAAGGGGGTTTTGCACTAAGGAGTCTGGGCTCATCCTAGAGCAGTGAGTAGCTATGAGAGGGCTCTGGCAGGGAGCAGTGAGTGATGGTTTTAAGACCATCAAGGAAGCTGGTGCATACCCAGAATCCTGAGCACTTGGGGGTGAGAGGCAGAAAGACCAGAAGTTCAAGGCAGCATCTTTACATTTATATAGAGCTTGAAATTGGTCTGGGTCATGTGAGACTCAGTCTCAAACAACTCTACCCTCCTGAAAAAAAAGTGTGTATATATACACATACACGTACACGTACACATATTAAAGTACTACCATTGGCAGGTAGGCAACTAAGCTGATGTTCCCACGTTGTGTAAAGAGACATCTTGTCTGATTTGCAGGGGCCTGAGATTTGGGGGTAAAACAGCTGACCCAGGTCACATGGCCTTCAGGTGCCTGTTCAGGCGACAACTTGGGATCTAGGCAGACTGAGAACTCTAGAGCCTGTAGACCCTTTGTCCCTTACTACTCCCTCCATCTTACAGAAGCAAATGCCCATGGGAAAACCAAAGGTGAGAAATCGAAGCCCTTGGCCAGCAAGGTAGGTAGGTGTCTGTTGCCCACTGTGTCTCAAGTCCTTCCAGGCTTGTGTGGGGGCTCTGGGAAGGAAGCCTGTGCCTTTGTGCTTGTGAGGTAAATGCTCTGCTGGCTTGAGTTATCTCCCCACCACTCTTTTTGCTTTTTTACAGGTCCAGAATAGCGTTGCTTCCCTCGCCAAAAGGAAGATGGCAGACATGGCCCACACTGTGACAGTTGTTCAGGGGGCTGAGACAGTACAGGAGACCAAAGTGCCCACTCCTTCCCAGGACATAGGACACAAAGTACAACCCATACCTAGGGTCAGGAAGGCAAAGACCCCTGAGAACACTCAGGCCTGAGTTACTTCCCAAGACCTCCTCCAAGGCTCCCAGCAAGAAGGCTGAGGCTAGTAGCTAGGGCCAGGGCTGGGGAGATGGCGATTCTGAAGCTTTTATTGTCTAATAAGCTGTCACAATGTTTAATCATAATTTATCAATAAAGACTTTGTATTCACACACTGGTACATTGGACACTGGACTGTGAGACAGGAAGGCAGAGGCGGGTTTTGAGCAGTTGGGAGCCAGGGAGCAGTGGTTTAGTTGATGTCTTTACAAAGAGCCTCCTGCCTGCAGGAGGGATGGAGGACGTGAGGGGCCAGAGAGGGTAAGCTTCCTGAGCCGAACCATCAAGCCTCAGCCCAGAGAGGCAGACAGACTACGGCTTGGGGGAGCAGGATATTGCCTGCCAAGGAACACAGTGCTGGCTGCAACTGTAGTATGTATTCGTGTACCTGTGTCATGGTGTGTGTATGGAGGTCAGACTACAATTTGAAGGGGTCCTCTCCTTCCACTTTGTGAATCCCGATGGAATTTGGGGTATCAAGCACCTTTGTCCAGGGAACCATCTTGCCAGCCCTTGGTGCAATTTTTGATAGGGGAGGGCCGCTGAGCAGGGACCCCAAGTCTGGATGGAATCGTATTCAGCAAGAGAAGGAAAGGGCACAAAGGCCCTGTATTGGTTACTGTCCATGTTGCTGTGATAAAATCCCGGACAGGAGAGAGTTAAGCATGGGAAGGGTTTACTTTAGCTTGTGGTCCATCACGGCAGAAAAGGCCCAGCAGCGGGAGTGCAAGCTCAACTCTTGCTCCTCTTGACTCCATGTGGGACTCCAGCCCACAAGATGGTTCCACCAAAACTCTGGTCTTCCCACATAAACATCTCTGGAAGGAAATGGCCTCATAGATATGACTGGTGGGGTGTCCCTAGGCAATTCCAAATCTGGTCAAGTTGGTAATCCACCAGTGGCCCTAGGGCAGGGATACGAGTGAGACAAGTCATGGGGCCAAATTCTGCACTGCTTTAAAATCATCAGAGACACTTTTCCATGGATAGGTGGAGTTAGTTCCCTCACCCTGAGTCTGAGCTCTGCATTGCTTGACCAGAGGGCAGTGGCAATGTCAGGACAGATCTGAGACTTCAGAGGAGGCAGGGTGGCTCAGTGGGTAAAGTGTTGGCTACATAAACATGAGGGCTAGAGTTCAAATCCCCACCACTCGCATAAAAAGCTGGGTGTGGTAGTGCATGTGTATAAATAACCCCAGTGTCAGGTGGTGGAGGCAGGAGGATCAACCCCCAGGGCTCACTACCCAGATAGCCTAGGCTAAAACAGCAAACTACGGGTGAAAGAGTCAAAGCTCTCAAAAAAAAAAGGTGGTCCAATGGTTATAGCCAAAGCACTGGCTGTGTATGAGACCAGAGTTTGTCCCCCCAGAACCACATGAGTGCCAAGTGAGTGTTGTGCACCCCATTAATTTCAGCCTCAGTAGGTGGAGATTGTGTCCTGGCTTGTAGGACTGTTTTTAGCTTTGGTCAAGCTGGAGTCACATGAGGAGAAAACTCAGTTGAGAGAACATCCCTACCAGATTTACCTGTGGGGAGTTCTCTTGACTGATTAATATGGGAGGGCGGGCCCAGGTCTCTGTGGACAGCACTCCTGGGCTAGTGCTCCTAGGTTGTATAAGAAAGCAGCCTGAGCAAGGCGATAAGCAGGTTCCCTCCATGGCTTCTGCTTCAGTTCCTGCCTTGAGTTCCTGTCTTGACTTCCTTCAGTGATAAACTGTAAAGCTGTGAGCTGAAATACATCCTTTCTCCCCAAGTTGCTTTTGGTCATGGTGTTTCATCACAACAATAGAAACCTTAACTTCAATGGGACCCTTAGAGCAGGCTGGTTAAAAAGACCACTATCCGAAGCTCTGCATTTGATTAAGACTACCTTGGAGAAGCGGGGCATGGTGGTTCACGCCTTTAATCCTAGCACTCGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGTCAGGGCTATACAAAGAAACCCTGTCTCGAAAAACAAAACAAAACAAAACAAAAACAAACAAAAAAGACTGCCTTGGAGAAAGCGATTGATGATTCTTAACATCAACCTTGGGCCTGCACATGCAACCCCCACATGTGTGCCTAATGGGATGCAAACAAGGTAGAGAAGTGATCAAGGAAGGAAGCCACCTGTGTCAACCTCTGGACTCCACACATACACAAATAGGTTAAGTGTACCTACACATACACACACCCATGTGTGCGCACATACGTGCACACATGCACACTTTTGGAGGTTCTTGTATTTTCATAGACATCCCTCATGCTGTAAGGAAGCTCACACTACCCATGGAGAGCCAAGGCACCCAGTTCACCTGCTGAGCTTCCGGTCACTACTGCCATAGTCATGCACCACCTTCCAACTCTCTCTGCTGACACGGGCTAGAGAGGGACAATGTCCCCATGACAAATGCCTAAGCAAATGGCTTGTGCTGCTCTTGGCATCTGAACATTGGAATGATGTGAACGGACTCACAGGATGGGGCTGCTGGCAGGCACTGCACCATGAAGGGTGGGTGTCACAAAGGATGGGCTCTGTGGGTTTACTGTCACGGTTGTAAAGAAAGATCAAAGAATAGGAGGAGGGACCCTAGGAGCATGGCAGATGGACGTATCTCCAGAGCACAGATGAGGGAAGAGGTGGCCTTCAATATGGTAGGTGAGGCTTGGTGGTAGTCCAACGGAGAAAGGGGTCAAAGATATGTCCATAGTTCTGGGTCTGCTTGGTGTGGTGCGTACTCTGCCAAGGCCAAGAGTGTAGTTCAAAGGAATGGGTGTATCTGAGGGGCATTCTCAATAGGGGAGACGGGACACTTGGGAAGGGAACAGACAGCACTAGGGAAACCAAGTGCCCAGTATGGGGCTCAGGCTTCGATGCTGTCCATGGCTTCTCTCTGGAGTCCCATAGGAAGCCATGTCTAGTTCTTCTGGCTCCCTGGGGTATCTATAATGTGCTTGTCAGCAGGCCCCGAGGGGCACCCAACAGATGGCAGGCTGGCATTTCTCAAGCAGAGGCTCCGATTTTTGCCTCTGTTCCTGCATGGTGAGGCTGGGGAAGAGCCTGTAGCCTCTTGCCTCCAGGAGCTAGGGACCCATCAGCCCTGCCCCGTGACAGGGTGGGGATAGACCTTGCACCGTCTTAGAGACTTGGGAGAACAGCTGCGTTGGTTTCTCCCACTTTGCAATCTTAGCTTGAGGATGCTCCAGCCAGGTCCTTGCCAGGGTACCCATGGCTGCCAGGCATGTCTTTGGGGCCCCACACAAGGACTGCAGTTCATGGGGGTTGCCTACCTGGCAATGCTAGTGACCCTGGAAACTAGATCTACGCATAGACCTCCTTCTGAATGTCAGGCGCTGTATGTTAATGAGACGTGTAGCCAGACTGTGGAATACTTAATGACTTAGAAAGATGTTCCTGACATATTGTTGTGCAAGGGAAAAGTCAGGTTTTAGAACAGAGTGTGGACTGATTCATCTTCACTCCTGGATCCACTACAGCAAAGGTGCCCTGGCCTTGTGTGTGGCCGGTGCCGTGTGCGAGTGTCCCCACAAGCATGCGAGGCTTTTATTCTCTCGGGCTATTTCTGCAGTTGTAGTTTTCAGGGTTTTGTGAATCCATTCTGGAGTCACACCTCATCGAGGAAAAAAATTCAAACCATCAAATCTGCCAATAATTGAAATTAGCAAATCTGCTCCTTAATAAGCCTCAGCAACAGAGATCTGAGAGATAAAGATGGTTTCTCCGCCCGCGGCCCGCACAGGAGAAAGCTATTTTTAGAGAGGTTGTTCACTTTTTTTTTTTTAGCTGGTGAATTCTAGGCTGGGTCTTGGACTTTGTTGTTGGGAATTTTGGTCCTGGCTTGCTTTTGGCCAGTACGATGGGGACTTTCCTTCTTGAGGGGACACATGTGAATGGCCATCATTCAGGCTGGCTCTGCCACATCCTGCTTCTGGGGAGAACCTCACTGACTCAGCCTTGAGAAACTGGGGCCCCAGTCGGGACAGTGGGTGTCACCCCAGGTCAGATGTGCTGTGGTCAGGTGCAGGCCACGGCTGGCCTCGGAGAGAAACTGAGTTTCCTTGTCTGAAACATGAGGCGTATGTCCTAAGAGTAGATGGAAGATTTGGGGGTGTGACTCTGGTCAGTTTGGAACCCCTTCCACTTTGGAGGTGGAGACTCTCCTGGAGGAACTTGGGCTGAGAGAAGGGGATCCAGATGTCTGGTCCCCATCTTGCCCTTCTTGGCTGTGACCTCTTGAGCCCCTTTAGGCCCCTTGACACTGTCTGCAAAATGAGGAACAAGATGTCTGCTTCTCTTAGGTTTGAATAAAGACAAGGGTGTGGGGGCTGGATGTGGCTCACTGGAGAGTGTACACTTAAGACAGCACAGGGCTCTGGGTTCCATGCTCTGCACAGCACAAATGTGAGGGGCACCCACTGGCGATATCCTCCTAGGGGTCAGGACTGTCCATGGAGATGTGTGTCCACAGGGTGCAGCCGCAAACTCCTGGCAGGCCTAAGGGAGAGGTCTTCCCTTAGGCCGTGTCCGAGGCAGGGTGTCAGGTCAGGCTCTGCACAGACAGGACGAAGAAACAGCCCCATCTCCCCTCCTCGGCCATCTCAGGACAAAGGCTAAAGCCCATAGTAGGTTTTCCCGGCAGGCTTTTATTATAAAGAATGTTCCAGAGAGTCCCAGCCTTCCCCTCCAGCCCAGCCCCCTTGGGCTGTGAGCCAGTCTCAGCAGCTATGGGGGGTGGGGTGGGGGCTGTGCTGGGGACAGATGAGTATTTTCTGTCCCCTGGAACAGTCATTTCCAGTGTGTCACCGGGAGCCCCTGGGGATCTGCACAAGCGGCAGCTATTCACAGTTGGTGCATAAGTTTTTGTTTTAATATATAAAAGTTTTTTTTTCTTAAAAATCAAAAAAAAGTGGTGCTCTTTAGAAACACTTCCAGGAAGACTGAGTAGTCCAGCTACAGCCCTGCATCCTCGCCTTCCTCCCTTTCCTGGGGCCAGGAACCCCCCAGCCTGTCCCCCTCTCTCCACCGTTCTGTGGGCAATGGGCACTGCCCATGCTGCCACTGGCCAATGAGACGGCTAACAGCTGACCTGGTTTGGAACTGTTGGTGGCCAAGGCCCCCTCTGGATGAGGGACACAGACAACTGCTTGGTGGCTGAAGCAGAAATTCCCTCTGCCAGGTGGATGAGGTAGTGTTCTGGGATATGGCCACCTCTCCCAATGTGTGCTGAGCAGCCTGAAACAGTGAACAGGCAGGCATGGTCCAGGCTGTCCTGGTTGGCAGGGAAAGTCTTATTAGGGACTGGCAGCGATCGATATTCAAGGCCTTCATCCTAGAGTTGGGTCTTGGGCAGGTGGAGGGGACAGAGATGCCCCAGGGGACAAGGCCAAGAGCTCGGCAGCCGGGCACTAAGAGGCCAGTGTCCCCCACTCCTAGCCCAGCAGGCTCCTCTGTCAGTTCCCAGCCCAAGTGAGAGATGTGGGGAAGAAACCAAGTGGAGGCCAAGTGGCCTGCACGCCGTCTCCCTGGCCGAAGCTCCTGGTCACTCTTCTGCATCAGGCCTCGCTGTAACACTCATAGATATTGTTTTCCATCAGAGCCACCACCTGTTCAAACTTGTGCTGTAGCTGGGTCCTGCGGGCTGTGGGGTTGGCCTCCAGGGCAGCCATGATCTGAGGGGAGTTGCAGTGAGTCAGATGTGCCGTGCCTGAGAGCCTGGGAGGCCACCCGCCAGAGACCTCATCAAAGTGCCAACACCCCCGACAAATCTAAAGCGTTGTTATGGGCTAAAATGGGCAAATCTTGGGAGGGGGAACTCACCTGTGGTCGATACCTCTTAGCATATTTATAAATCTCAGCCATGGCCACGTTTGTGTTGAACTCATTCTGGTATTTCTATAAAGGTGGCACAAGAAGAAAGGGGAGGTATTTTTACTTGTTTGCTTGAGGCAGGGTTTCTCTGCATAGCCCCCTGGCTGTCCTGAAACTTGCTCTGTAGACTAGGCTGGCCCCGGACTCAGAAATCCACCCGCCTCTACCTCCCAAGTGCTGGGATTAAAGGTGTGTGCCACTACCAACTAGCAAAAGGAGAGGTTTAACCCTACAGATGAGGACCCAGCCTGTTCACTCCCACAGCCTGCTGATAGAATACATTTAGACCCCTGCGAGCTGGGCACACACTGGCTTTCTGGAACCAGGATTCTAACCTCTCATATCTTCAGAACACTCCACCTCATCCTTGGACTTTAGAAAATACATTGCAATGAAGATGCCAGGAAAGATGGGAAACAGAGACTACCCCCAAATTCCTACTGTCCCCCTGCTGCATCCTAATGGTGCTGGTTTTGTCCACTCCTCAAAGCCAAGGATATCGTTACTATCTCCTGGCCTAGATCCCAAAGGCCACCTACCCGAGACTCCTCGGCCAGGTGTGCGTTCATTTCCTGCTCGCTGAGCGGCGTCATGTCTTGGATCTGTTTATAATAGCGCTGTACGGTCTTCCGGTACTCAGGGATCTCCTTCGCGTACAGAAGCTTGTTGGTGGGTGAGTCCTGGGCAGGGAGACCCGGGCAGTTTGGGTGGCTGAAAGATGGGACTTGTCCCCACTGCCACCTAGAGGGCCTTGGTGGCTCCTCAGACATGGCTAAACAACCCCCATTCAGTCAGACTTTGGACCAGGGTCAGTTGGTCTCTTCTGGCCCTCCCTCCCTCCCTCCCTCTTTCCCTCTTGCATGGAGGCCCTGCCCCACGATGCTGTCAGGCTTTCAGGGTGGTGACCACATGGGGACTTTCATTTGAAGGGAAGAGCTCCTAGGCTGTGTGCCAGCAGTACTCCGCTGCACGCAGCCCGCAGCCTGTCTTGTTTACTCCCTTATTAGGGTGATAGTATTTGGTGGCTGAGATGGTTGGAAGCTGGTTCCTGACTGCGGGAATGGTTCCCACCCTGCACTTCCAACACCTCAAAAGCACCATGTGCTAAGACGTATGCTGGCAGCTCTCTGTTGTCACGGGAATGCATGTGTGAAGTGGTGACATTTCATTTCCCTTGTGGGCTGAGACTCCTGAGCCCTGCCCTCACCCCCCTCCCCCCAGCCAGCCTAGCTCTGTGATCCCATGGGCACTCATGGCCTCAGAGTTCTGGGCTACCTAGGTCACCTCTCTTCTCAGAAGGCCTTTATGGCTTCTAGCCACTGAACCGGCATATCCTCTGCCCTTGGCAGGTGCCGTGCCTGTGGAGCTCGCTCTGGCTGGCCATCTGCTCTCTGCCTGTCTTGAATGACTCCGGTAGAGTTCAGGCACCCGCCCCCCACCCCCCCTTCAGGAGCAGCCAGACTAGTTAACCTTGCCCAGCTGCAGGTCAGAGATGGAGCAGGCATCGATGAAGGCCTGTGCGATGACAGACAGGCAGGCGTCGATGTGGTCCGTCTTCTCTATGTCGAAGACAAACTGGGGATTTTTTAAGATGTTCACCCAGAAGCGCAGGGGAAGGCTGTGGGGGCAGGGACAGTGGGGATGCAGGAGCTCAGTGGAGGGCTGGGGAGGTAGACAATGCTGGTCCTCGCAGCCCCTGAAGCCTCTGGATATGTGGTGTCCTTCCCCTGCCAGCTAGGGGCCAACCCTCACCCCAGAGAGCCTTCCTCTCCTTTGCACGCACACGAGACTCCTGGTTCTCATTAGAAGCCCCCACCCTGCTGCCTGTATCCACACTCCTCCCCCACCCGGCCTTGCACGAAGAAGGCAGGGCTGACTGTCCTTGGGAGGCCAGAGAGGGTTGGGGACAAGCCCCTGCTCAGCCCTGCAGAGCCTGGGGTGCCTGCCTGCCCAGCCCCACCCCGAGCAGAGAGAAACTGCAACTGAATTTGCACAGGCATTTCTCTGAGGGCCAGGCTAAGAGTTCTGGGACCTTCTTGGCGGGGTAGTATATGACCCCAGAAAGCGAAGAAGGGGCCTCAAAGGAAGCTACATATTAGGTGTGGGAGTCACAGCCAATGTGCACTGCCACTTCCCAGTGTGTGCCCTTGGGCCTGGGTGACTGCTGCTCTGTGGGCCTTCGCTTCTCTCTGTATGATGGGGCATTAGCGGTGAGCAAAGGCAAGCTTATATGATGCTTTCTGGTTTCACAGAGGGGACACTGCAGCACCAGAGCTAAGTCTCTCACCGGAGACCACACAGTGGGGAAATGGCCAAATAGAAGCTGATTCAAGACAGGAAGCATTCAGGCCAGGCTCTGCCCTCATCTGCCCTGGTGCTTTGGCTGCCCTGATGCCATGATACCATTGGTGCTCTAACCCCATTGGCGCCCCCCCCCTCACCCCCGTGCAACCCCATTCCTCGGCCACCACCTGTTGGTCTTCCAGATATGCAGGGTGTCAGGGTCGGAGATGCCTCTCTTCTCCGCCTGTTCCTCTAGGAAGTCAAAGAAATACTTGACAGCCAGCGGGGGCTTGTCCTCTCGGATGCTCAGGATAGCCTTGAACAGGTCATCTAGGAACTTCTGCAGCGTGCCCTGCAGGGAAGATGTGGGACATCATTAGCCCAGCCTCTGAACATCTCTGAGCCTCAAGATTCCTGACTGAGTTGTCAATGATGCTTTCTTGGAGCGGGGGAGAGGAGGACCTGGGTAGCAGGGAGGTAGGGCTCAGAATGTGGCCTCTGGGTGTAGGTCTGAGTCTTGTGACTTGAGGCCACGGAAGTAACCCTGGGTCCTCTGCTGCCTACTGGGTGGCAGGAAGAACACGGACTTCTTCTTGGAGCACGGGGACCCCTTTATTCCCCTACGGGGTGGACATGGAGGCTGGGCCCACCTTGGTGGACAGCAGGCGGGTCAGGTAGATCTCTGGCAATACTTTCTTGCGGTGGCTCTGCCGGTGAGATTTCTTAGGCTCTACCAGCTCATCCGTAGGTAGCACCTGCAAGGTGGGCAGGGTTGGTCCCCAACGGCACCCTCCTCAATCCTCCCACGGAAGTCTGCCCTGTGTTACCCAGCACCCGGCCCATCCCTGCTCCCTCACCCACTCTGGCTTTTCCCAGGGCCTAGGAGCTCTTTCCTGGAGGCTCAGGGAGTCAGAGAGCCAAAGCCCCGCCCAGCAAAGCCTGCTCCCCAGGGCCTGAGGCTGGGCACTCACCAAATGGAAATACTTTTCTGTGTCCAAGTCTTTCACTGCAAAAGGGAAACACAGGATAAACAAATGAGGGGGGTTGGGAAGGTAACCCGAGGGGGATGGCGAGAGGGGAAGACTGGGGGAGAGCCAAGATCTGGCGTTCTACTTCAGGATGGACCCTGGGACCCTCATGGATCTGGGCCTAGGGCCTGTAAGGACATGGTCTAGATTTGGAGGCAGGGGTGTGGCCTAGAGCCCACAGGTCATGTAACCAGCTCAGACCTGGCCACGGCCCAGGCCTCTTGGAAGGGCACGTGCCCTCCTGGGGCCTTGCTGTAGCTGCTGGAAGCAATCAGCTTTTCTCTAGCAGCATGGAGGTGGGGACCCCCTTTCCAAAACTCAGTTGCCCCCCACCCAGCACGGCTCTGAAAGGGCCCAAGAAGGGAGCCCTCACATCACGCTTGGGTCACCTCCCCAGCTATGAGCCATACCTCTGCCCAGGGTACTGTCCTTCTTGTCTGTGAGGCTCATGGCTAGGGAGGCGCCCTCAGGTATCTGACAGAGGAGAGGAGATGAGGTTAGACGTATGAGGTTAGAGGGGAGGGGTAGGGTGGCTGGGCCAGCTTAGGCACCTTGTAGTGGGCCAGTGTGTTCAGTTTCTTACGGCCGTCCTCCACCACTGATGTGTCATCCAGGTCCCGGAGGACGTAGCTCTGGGTACTCGAGGCAAACCATTCTGGAGGTGAAGAGGGGCATCAGGACTACAGAAGAGGTGGCCCCAGGGGCAGCCACTAGCCTGGACCACAGTAAAAGAAACTTCCCATTCTCCTAGAATCAGAAGCAAGCGACGCCCCCTGGGAGCCCGGCTGTTCACCCTAACTGTCCAGAGTACAGGGCAGGGCCTAGTTCAGGGTGAACTTTAGGAACAGTTTCTGGTAACCGGTCTGACCACGACTCCTACGCATCCTTCAAAACCCAACCCGAAAGACCTCTTTGGAAAGCGTTTCTCGATTGCTTTTTGCTGTTGCCCACAGCTGCAGAACAAGGGTTTGTTCCTCAAGGGGAGCCCTGGGGCCTTGACTAACACAGGAAAGAGGCTGCAGCAGCCAGCTGCGAGGCATGGTGCCCGCCCTTGCCCACCAAGGTCCACGTCCTCCGCCCGCGGCCACTGTGAGTAGGGGACGTTCTTGCAGAAGGCTTCCAGGATCTTCTCCTTCACCTGCGTCAGCGTGTCGGTGTCCATGGCCCGCACGCTGAGGGAGTCCATCCCACAGCCCTGGAAGGACACGTTCAAGTTCTGCGCAAGAGAGGGGGGTGTCAGCCGATGGCTACTGTCATGATAAGGCCTGGTGACCTGGCTGGGGAGGCTCAGGATAGCCCAGCTAAGAATGCCCTCCTGCTTCGGGCCCCGGGCCCTGCCCCCGGCTCACCCGGGGCTTGGCCTCAATGTTCTCCCTCAGCAGCCACTCCTCGTTGAGTGTGTAGCGGGCTTTGCCTGTGATGGCGTCGATGGAGCCTTTGTTGATCTGCTGCTTGATGGCACACAACAGCAGGAAGAACGGCTCACCTACTGTCTCCTGTGAGGGTCAGGGGTCAGGGGTCAGGACCCCCTCTGTGTTCCAGCTTCCAAGCTCTGGCCTGCTGGTATCCCCCACACTCCCTTCTACTGATGCTGACACCCACTCCAGGGTGTCTCCACTACATCCCAGCCACATACCCAGAGACCAGCCCTTGTTGCTGAAGCTCAGAGAGGGCAGGCACCCTGCACAGCAATGCATAGCAAGAACAGGTAACATGGGACTTGACCTACGAACTCCTATGCCTGAGATGTTGGAGCCTTCCAGGGTGTAGCTGAGTGGAAATGGGGCTGAGGAGCCCCCAGGGAACCCCATATGGATGCCTCTGGCGAGTGTTGGCCCAATCAGGCTTCCCACCCCACCTCCTGCCCTGGCCACTGCCATTACTGCAGCCCCAAGCTAGCCCTCCTCGTCCCCCTAGACTAGTGCCCAGCTGTTCTGTGGGTGGGGGAACGGAACGACCCGTGGTGAATTGGGCTGGAGGAAAAATTAAGTTTGTAATAACATCAGGCTGCTTTGGCAGCAGCGTCAGGGCAGCTGCCCGCCCAGCTTCCTGCTGTAGTGGTCTGGCCTGGAGTCCCCAGCACCCCAATGTCTGGCAGGGGAAAGGCCAGGCTGAGGTCACCCACAGTGGCTGAGAAACCTAGGTCCAGAGTCTATGGTGGTCCCTGAGAGCTCAAAGTGCGGCTTCTCTCCTACGATCTCCATTTCTTTTCTACTCTCTGACTGACCCTCATTTGACAAAATGAAGCTAAGGACCAGAGAGGGTAATAACTCGCCCAGAACTACACAGCCAGGTTGCCACAAAGACTAGTACACAAACCCAGAACAGTTAAGGCTCTGGTTCTGGGTCCTATCTCAGGATTCCGAGGTGAGTGCTGGGCCCTCTGCCACCATTGAGCAGGCCCTTGAATTGTGCTTCATGATGGCCTGACTGGCCTAACTTCAGAGAGAGAGGCCCTGGCTGCTGAAAACTCTGTGATGTCTCCTAGTCCTGATCTTTGTCCACAATGTAGCCTACATCTGGACTGCCTTGTTGGCCCCAAAGCCCTGTCTTTTTTCCACGGCTCCCCTGGGGTGCAGGATGAGTCACCTGCTCCAGGAAACCTCTTGTCTTTTTCCAACCAACAATCAGCCAGGCTGGGCCAACCTCTGTTCTGTTGAGGTTTTGCTTCTGTGCCCCTCCCCTAAGGAGAGGAAAGCTCAGGATTGAGGGACCTGACCCTCCTCCAAAGTCCAAAGACCCTCTGCTTGCATCTCCCCCGCCTCGGACCCCCCAAGCTTAGGACTAAGGCTACAGGACAAGACTCAGCCTCCAACTTGCCCTGGAGATGCTGTGTGCCTCTGGAGCCAGCTAGCCAGATCTCCAAGCTTCAGTTTTCCCCACTGTCAAGATGTGACCCCCTCCCTAGGTTTTCCAGTTGGCGGTGGGGGGGGGGCGGCGGAGGTGGGGGCTTCTGGGCAGAGAGGGGACTCACCCTCAGGCAGCCGTACATGCAGATGGACATCCAGTTGGTAAGCATCTTCTCCACCACAGACTCCGTGCGCCGCAACATGAGCTTGGGGTTCTTGGCCGCCGAGGCGTCGATGAGGTCCACGAGCAGCTCCTTCATGATGCTCGTATAGTACTCCAGCTTGCCGTGCAGCGCGATGGTCAGCAGGGACGCCAGGCTGCACCTGCACGCACAGTGTCAGCAAAGCTGAGCATCCCCCTGGACCAGAGTGCACAGCGCCTCCTACAGACGGCCAGAGGACTCTGCCTCCGCTGGGAGCCGGTGTGGCCAATGAGGAATGCCTGGGGAGGGGGGTGGGGTCCAGAGTCTCAGCCTGACCTGTCACGCACTGCGAAGTCCTTCTGCTGCTCCAGAGCATGGACGAAGACGATGAGGAAGTGCTTGTTGTTGAGCAGTGAGGAGAACAGGCTGATCCCCTCCTCCATGCTGGGCCGACAGTGTTCAGGGATCTGTGGGAGTGGAAGGAAACGCCCTATGGTTTGTGTCCCAGGAGGCCCCAGTACCCATGCTGGCTGTGTAGCTGCCTCCTGGAATAGGACGTTCACTACCTGCCACAAAGTGTCCCCCCTTAACCTCTCCTACTGAAGAGTCAGAGGAGCAGCAAGGTGACAAAAGGCCACAAGGACAGTGGCAGGGAGCCCCATATCCACTCTGTGCCAGTTTCTATCTAACCAGCGGTGGCATGACATCATGTGATCATGTGTCCGTGCTCTCTGCCAGGTCGGGGGGGTGGGGTGGGAGGGAACAACAGCACCCTGAACGGCTGAGTGAATGCCTCCTAGAAGGGAAGGTA
GF3 -1 -1 1 GTGTGTGGTATGTGTGTATGCATGCATGTG
GF4 -1 -1 1 GTGTGTGTGGTATGTGTGTATGCATGCATG
EX52 -1 -1 1 CAAGTACCTGTTGAAGCAAGCTCTG
EX32 -1 -1 1 CAGAGCTTGCTTCAACAGGTACTTG
GR3 -1 -1 1 CACCAAGCCTCACCTACCATATTGAAGGC
GR4 -1 -1 1 GAAGATGAATCAGTCCACACTCTG
Comments:
Created
Target: 0402Ensembl ENSMUSG00000042279 -- 115897653 -- 115897912 ENSMUSE00000307150 -- ENSMUSE00000307150
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: 6, start 115897653: end 115897912
Found 10 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:6:115891153:115892152_636 G3:6:115901412:115902411_847
ACCEPT (0) gap pair G5:6:115891153:115892152_636 G3:6:115901412:115902411_559
ACCEPT (0) gap pair G5:6:115891153:115892152_636 G3:6:115901412:115902411_210
REJECT (2) gap pair G5:6:115891153:115892152_64 G3:6:115901412:115902411_847
ACCEPT (0) gap pair G5:6:115891153:115892152_64 G3:6:115901412:115902411_559
REJECT (2) gap pair G5:6:115891153:115892152_64 G3:6:115901412:115902411_210
REJECT (1) gap pair G5:6:115891153:115892152_631 G3:6:115901412:115902411_847
ACCEPT (0) gap pair G5:6:115891153:115892152_631 G3:6:115901412:115902411_559
REJECT (1) gap pair G5:6:115891153:115892152_631 G3:6:115901412:115902411_210
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

webmaster@sanger.ac.uk

Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK  Tel:+44 (0)1223 834244