High Throughput Gene Targeting: Create Design

This custom vector design tool is provided by the EUCOMM consortium as a service to the research community. When using this tool, the user agrees not to submit more than five designs per day for creation without prior discussion with the High Throughput Gene Targeting Group at the Sanger Institute.

Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by vvi
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 84673
Chr: 10
Strand: -1
Status: Ordered
BACs: RP24-236O23 : RP24-257L23 : RP24-265J14 : RP24-341K21 : RP24-285C5 : RP24-287I17 :
Instance: Plate 88: Well E09: BACs RP24-257L23 RP24-341K21 RP24-265J14 RP24-236O23
Oligos on GRCm38:
Type Start End Length Seq
G5 127192968 127193017 50 GGAGTGGGGCTGCTCGGAGGGGAGGAGGGCCTGGGCGGTGCAGCTCGGGG
U5 127187744 127187793 50 TTACTATATATATTGTTTTGGACAGCCCCCCTCTCCAGCGCATGCACATA
U3 127187631 127187680 50 TTCCCTACAACACTCCCCCTTGCAATAGGTACAGACCATTCTCCCGGAGA
D5 127186660 127186709 50 ATACTGGTAGTTGTGAATCATGTGACATGGATGCTAAGAACTGAGGTTGG
D3 127186571 127186620 50 TGATGATTATTACCATTTTTACATTATGGGGGGCCATGTGTAGATACCCA
G3 127182531 127182580 50 AGCAAAACTTTTATTCACAAGAAAACTCGAAAACAAAATCACAACCCAGG
Oligos:
Type Start End Length Seq
D5_D3 126623677 126623715 39 CAGAGACCAGAAGAGGGTGCTGGTGGTTTAGAGCTGGAG
G5 126630024 126630073 50 GGAGTGGGGCTGCTCGGAGGGGAGGAGGGCCTGGGCGGTGCAGCTCGGGG
U5 126624800 126624849 50 TTACTATATATATTGTTTTGGACAGCCCCCCTCTCCAGCGCATGCACATA
U3 126624687 126624736 50 TTCCCTACAACACTCCCCCTTGCAATAGGTACAGACCATTCTCCCGGAGA
D5 126623716 126623765 50 ATACTGGTAGTTGTGAATCATGTGACATGGATGCTAAGAACTGAGGTTGG
D3 126623627 126623676 50 TGATGATTATTACCATTTTTACATTATGGGGGGCCATGTGTAGATACCCA
G3 126619587 126619636 50 AGCAAAACTTTTATTCACAAGAAAACTCGAAAACAAAATCACAACCCAGG
G5_U5 126624800 126630073 5274 TTACTATATATATTGTTTTGGACAGCCCCCCTCTCCAGCGCATGCACATACCCAACCCAGGGGGCAGTGAGGACAGCTCAGCGGGGCTATCTTTGGCCAGCAATTGGGAAGAGCTGCAATGTGAGTTCTTATAAAACCCTTTACAGGTAGGTAGATACCCCTCGCCCCACCCCCAGCCCACCATTCCTCTGCTCCCTTTTCGGAAGATATTCAGCCAGTGTGATCTGAGACACAAGGTTCTGGGCATCAGGCGATCACCAGAGAATTTTCCCTCCTGGCTAGCTGACTAAAGGAAAGAAATGGGTCCTCTGACCCTTCCTCAACTTCCTCAACTTGAATCAGCAGGAATCTAGAAGTGGAAAAGGACCTTAGATATTCTCTGTTAAATCATTTTTTTTCTGGAAAACACCCGTACATGCTCCAGCCCTAAAGCCCTAGGGCTAGAGTAAGCAGATGCCCTAACCCCAGCTCAAGCTCAATGCTGGCCCCCTCAGACCTCGCACTCACCACGTCCCCCCCGGGAGAAGCAGCAGCCGCATTTTGCCAGCACTTTTCGAATCACACGGGGACAGGCACAGCGACCCCCTTTGTGACCCCCCCGCATGGCGCCCGGGGCCCCCCGCACCCCCCACCCGGGCCGGCCATGCAGGGGGAATCACGGGGGGCGGGGCGGGGCGGGCGCAGGGCGCACACCCCCCTCCCTCCCTCCGGGGTCCAGGCTTGGAGAGGGGGGGAGAAAAAGGATGGGAGGCGGGGGTCCAGGAGTAGACGGAAGTCGAGGCAGGGGAGTCTGGAGGGAGGGCCGTGCGGGGCTGGGCCGCGGAGTTGGGCGGGGAGGGTTTCCGGAACCCCGGCGGTGCGGGGCCCAGGGTCCAGATGTCCAGCCAGAGGAAGGGGTGATGGGGGAGGCCCGGCCGGGATGCGACAGCCGCTCCAGCTCCGTCTCCGGCCTCGGCTTAGGGGTGGCTCTAGGCCCGGGCAGGGAGGTGCGGGGGCCGATTCCCAGAGCCACTGGAGCCGCGCGGGTCCGGGCCGAGCGGCGAGCGGAATAACAGGCCGGGCCACGGTGCTGCCGTGGGGCGGGGCCTCTGCGGCCAGGCTCCGCCCCTCCGCGCTTCCCCAGCCCCCTCCCCCAGGCACCTCCACGCTTGCAGTCCGGGTCCCCAGACCTCCGCCCCTCCTCCCAGGCCTGGGCTCCCTCTAGCATCCCAGCTGCAACTGCGGGGCTCTGGGTTGGGTGGGCGTGAAGGCTGGAGGTGGGTCTGCCTTGCTCGCCCCTTGGACTCTAAGATGAAGAATTCGCAGGATGGGGCCTTGTTGTGTGTGAATGGTTCTGAGGTTCTGGCTGTCTCCCCCGCCACCCCTCACCCCCATGCCCCGAGTAGGGCTTGTGTGCCTGGGGAACACTTTGTAGGGAACACAGCCCAGGTGCCGCCCTCTCCCCCGACCCATCTTTCCTCCAGGCCCCAGAACTCCAGCTAAAACTAATCACCAATTAAGGCGAAGTCCGGGCGCCTGGGCTGGCCCAGGGCCAGGGGTGGGGCGCGGGGACACTCCACTCCGCCTTCGGGGGCTCGGGACCCGCCGAAGTGAGGACACACATCGGGCTAGAAGAGAAGCGTGGTGTCTGCGATTTAATGGTCCCATGCAAATTAGGCGCAAGCCTTTATGCTAATATGAACAGAATCTATCTGAAGTCACCACTTTGCGCAGGATGGCAAATTGTGCCCCACCCAAGCCCAGCTTCTTACCGGGCTGCGGAGCCCAGGCGAGGACCTCAGGTTCGCGCTGCCCTGTCTGATCCTCCTCGATACAATCACTCTCTGTCTTGGTACTCCCAGGCCCTAAGGGATCTCTCTCCGGGGGCTGCCGAGACAGAGTCACTGTAAGGACCCTTTCCTAGGCTGCCACGACCTCCCCCTTCTTCTCTCCCTCCTGTCAAAATACACATTACTCTGAAAGCGTCAACCAGAACTTGGCATATGGCCCTCGTAACTGAATTTATGGATCCTTGCGACTGCTCCATATAACTTTTGTTTGCGGGGAAACTGAGTCGTGGAGTGCAATTCTCCTAAAGTCCTCTAAAGACCCCCCCGACCCCACCCGCGGTCCCTCCTCCTTACCCCCTCCTGCCCGGGTACGGCGCATGCGCGCAGCATGCCACCCATGACCCCCAGTATCCGGTCAGTGCGGCCAGGGGTGGGGCGGTCCGGGGGCTTCATGCTGGACCCCTACCCTGCTTCAAGTTTCAGGGCCGAGGGGCCACGGAAGCTGGGGGGCTCTGAGGCCTGCGTGCGTCCGGGGAGTTGCTACTGCCGAATCCGCCCCCCTGCCCGGCTCCGCCCCGGCTCCCACCGCAGCCAAACTTCTCAAAAGGGGTAGGGTTGTTCCCTTCTCCACAAACGTAGCTGAACCACAAACAAACCCCTCTTAATCCTGATTCCATCAATCCAAGTGTGGCCTAGGGCTTTGCAGGGTTATTGCAACGCCCAGCGTTAGCTCTGCCAGAGGAGAGTGACTCCTAGGAAGAGAAAGGGTCCATTCAGGCATGCAAGGAATCCCCATTACCCCATCTCCTCAGCGGGTCCACACCCCACCCCCACCCTGTCCGAGGAACAGAAGACGGGTGTGGCATAGAGTTGCATATTTCACTTTATTTTTATTAAATTAAAAGCTACAGTCTGGCAGCAATTCCAGATTTGGGGGGGGGGGGCGGTGAGGAGGCTCCTTATAGGGGCAGAGAAGAGCGAGAGACAGACTGACAAAGACAGACACGGGAGAGAAAGGGTCCGTTAAGGTAGGACTACTGTTATCTTAACACACAGCTGGCTGGGGGAGGCAGCTAATATCAGTCCAAGGTGAGACAGTATTCAGGGGCTGGAGTTCCAGAGACCCCATCCTTCCTACCCAGGTCCTTTCTCAGCTTAGTCACTGGAGCACGGTACATACCAGGAAAAGCCAAGGACACTGGAGAAGCAGGGGCAGAGAAAATATGTACACGGAGTGAGGGGACGGGAGCCTTGGAAAACAGCCTCTGCTGGAGCACTTGGCCAGTGGGGGGGCAGGGGAAGGGACCATGTCCTCTTCCCCTCTGAGTTCCCACCCCAGTCAGACATATCCCCTCAAGGATGGTGTGGGGGCAGAGAGAGGCGGGGGGAGCAGAGGCTTCCGTTGGATCCTAGTAGAGGACAGCGGGGGGCCTTGGCAAAGGAGCTGTCCCTCAGTCGTCTGTGATACCCTTGGCTCTCAGTTCCTCTTGCACCCTAGTCAGGTAGGTTGGGTCCGGATACCCAAACCTGGGGCAAAGAGAAATGAGGATCAGAGTTAGTCGTTTGGACTTGATCGTCCTTACCCACCCTCATTTTGAGTGGCTCAGTTGGGGCCAAATTCAGACCCCTCCCACTTCCCATGAGGAGGGACCTCCAAAACCAAGATAACCCAGCCAGAATATGCTTTTTGGGTGGGGAGGGGAGGTGTTAAGCTGAACGCGTCAGGACCAGGATGAAAACAGACTGAGAGGGTGGATGGCTCCCCCAAATTAGAAATGGTGAAAGGGAGACGCCATGGAGAGGGAGATGAGGAGGAGTCGCACAGCTGGGGTCCCCCTGTGCAGCTGGTCTTGTGGTGGATGTCGTTCCAAGTGATGACATTGGGTCTCCCTGTAGTCATGGACGTGCCGATGGTGAAGGTGAGGCGCTGGTCAAATGCCTTTCGGAACAGGGTCAGCACCTTGTTGCCCTCGGGGCAGTCCGGGAGGTAGGCCACCCGTGTGGTACCAGGGTACCGAACTCCTGGGTTGGGGTGTTCAGCCTGAGAGGAAGAGGAGGCAGAGAGCAGCTGGCTGTCTCAAGGGCCTGGACACCAGCGAGGGGTCTGTCAGTCTGCCCTGCCTTTTGTCCGTGCATTCCCAGCAGACTGACAGAGAGACTTCCAGGGACCGGATGACTTTGGAGCCTGGGCAGGAAGAGGCTGTTTCTAGACTCAATCCTTATCTTTAATCCCTCACATCTCAAGTCAGGGACAAATGGACGTTCAGAAGTGATGGCAGTTACTTTCCAGATAAGTCAGCACAGCTGCAACAACTAAGAAAGATTGTATCTTTTCCCATTCCCTCCTTTTCCTAAAATACTACTGCCTCTGCCTCTTGTCTCCAATTTCTTCTTCTTCAGGGTTTCTCTGTTCCTGTGAATCAACTGGACATGACTCTTTGTCCAGCCGGAAGACATCTGCCTTCAGCTCTTATTGTCATATAGTTTGCAACAGAGCAGCACTCCCAGTGGTTAGCGCAGCCAGGTTCCCTTCCTGTTGTGCTGTGCCTTCCTTCCTTCACCTGGGCTTTGCCTCCACAAAGAGTCTGACACATCCACTCAAGTCAGGGTACTAGTGGGCAACCCTTTCTCTCATGACTGGTTTCAGGTTTTTTTTTTTGTTGTTTTTTTTTGTTTTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTGAGACAGGGTTTCTCTGTGTATCACTGGCTGTTCTGGAACTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCACCTGCTTCTTCCTCTCAAGTGCTGGGATTAAAGGTGTGCACCACCACCACTGCCTGGCCACACCTGAGTTTTACATCTGCCTGTGGGTCATGCCAATAAAACAGTGGGGCCTCAAGGAGATGGCCCCTCCCCAACAGCTCCAGAACATAAGTCCTAAGGCATGCTGGGAGACAGCAAGAGTCTAACATGTCTAACAACATACTCTCTACATTGCCTTTTTTTCTTAAGTCAAGAGACAGTGAACTTGGAGGAAGCCAACGGGTAGGAACTGTGATCTCTTACCCCCTGGACGCCAGGCGGGAAGACATATTGGATGACAATGGTGCCATACTTCTCGTAGCTGGGCAGCAGGAGGGTGGCATCCTTGGAGACCAGCATCCGTCCGTTCTGGGGCTGGTTGCCCACAAGCTGCCCGTAGAATCGGCCGCACATGGGGCAGGCCTTTTTCACCTGCAGGGCCCGTGTGATGCAGCCCTCACAGAAGGAGTGCCTGCACTTCTCCAGCGTCTTGGCATTCTGTATCTCCCCCAGGCAAATGGGGCAGGTGGTTTCCTGCTCTTCAGCGTCCTCTCTGAGACGAGGAGGGAGTGGAGGGGGCAGGGGAGGAGGAGGAGGGGGTAGCCCTCTTGCCCCTGGGGGCAGGAGTGGGGCTGCTCGGAGGGGAGGAGGGCCTGGGCGGTGCAGCTCGGGG
U3_D5 126623716 126624736 1021 ATACTGGTAGTTGTGAATCATGTGACATGGATGCTAAGAACTGAGGTTGGGCCCTCTGGAAGAGTGGGCAGTATCCTTAGCCACTGAGCCATGCCTCTGGTCCCTCACTGATTGATTTTGAAACGTGATTTCATACAGCACAAGAGAGCATGGGACTTGATCTGTAGCTGAGGCTGGCCTAAACAGGTGCCTGTCACACGCCCAGTTCTATTTCTTCACCTGTGCCTTGTCTCCAGGACCCTCCAGCAATGTAGTCAGTAAGGTCAGTTCGGGCAGCTCTTGTCCTGTGGTTAAAGTTGGCTCCAGCGTCCAGTTCTGGACAGAGAGAGATCAAGGGCAGACAGAGCCAGTGACTGAGGTGGTCAACAGTAGATGGGTTCCCCAAGGGCCAGTGACCCCAACCTGCACTCTTTCCCAGGGTCCTTCTTCTGTGGGACTCCCTGCTCACCTCACATTGTCCGGTCTGACCACTCTCTGCCTCAGTTTCCACACTGAGACAATGTTTGGAATGATCCAACCATCTCCTCAGGCCAGTTACTGGTCCAGGGGGAATCGGAGAACACGGATCAGAGCTGAGGCCAGAGCTGGGGCCAGACAGGGCAGCCAGCCCCGAGACAGCAGACGCTGACATGCTCCCCTCACTGACGGCAATAGAATAGGATTCTGATGGGAGGAAAACGACATGAGTGGATGGACTCTGCCCCCCACCTCGGGAGTCCTAGGGGAACCCCCATGTTGTCCTCTCCATGATGTTCAGCCCGTCTCCCAGGGTCCCCTCATTTGCCACCCTCCTTTGGGTTGGCCAGGATAGAAATCTTTGCAAAGGCACTCTGGGGAGGAGGGACAGTAAGCTCCCAGGCCCGTGCTCCTCCAGTTCCATGTGAAGGTTCCCCATATCCTGAGCATAGCACAGATATGCGGGTGGGGGCAGCCATCTGGGCAGACACTGGAAACTGGGGGTAGCCATCTGCTTCCCTACAACACTCCCCCTTGCAATAGGTACAGACCATTCTCCCGGAGA
D3_G3 126619587 126623676 4090 AGCAAAACTTTTATTCACAAGAAAACTCGAAAACAAAATCACAACCCAGGCCAATAATTTCTATCTGGTCCCTGCCCTCATCCTCCCTCCAAGCTCCTCCATATCCTCCATTTGAGTGGGTCGTGGTGCATGCAGGGAGTGGAACTGACGGATCCTGTGTCCCTTGGGCAGAAAAGAGTCCTTTCAGGCCCAGTCTCTCCCATCCTCTATACATCATGGAGGATGGATTCTGGCACATTTCCAGGATTCCCCCTCCAAATTCATTGAGGAATCATGCCTGCGCCAATAGCACTGCACTTTTGGGGTTACAGCTCATCTTCATCCAGCTTGGCGAGCCTGGCCTGACAGGGAGGAGTGTTCACAGTGGGAAGGGCTGGAGAGTCATGAGGAGTCTGGGGGGTATCACTGAGAGCCTGAAGGAAGATTCAAGTCAGAGGGTCAGCCAGGGTTACCAGTCAGAGGGTCAGCCAGGGCTACCAGCCTTCCCTCATTCCTCTCTCCAGTTAAAGACCCTAAAGATTCCTCTTGCCCCAGAAGGAAAGAAAGGAAAGTAAGAATGAAAATTTGGAAAGTCACACAAAAGGATAGTGGTGGAATAAGGAAATGGGGTGGGGGTGGAGACAAACGCCCTCCACTTTCTGCCCTAGTAGGAAACTATTCCCCATACCTGTGTATCCAGGGGCAACAGCACTCTGGACACCTCCCCTCTGGGCAGAAGTAGCAGGGAAGGGAGAGAGCCATTGATGGGTGTGTGCATGCTGGCCTGGGCCAGGTCTCCAGGCCGCATTCTCCCAGGGCTCCCTACCCAAGGCCCTCCTGGTCGCCCCAGGCTATTGGTCTGGCTCTCACGTCTCTGGTATTCAATGGGTGACTGTAATGCTGGGCCAAGGGAAGGAACAGAGATGTATTACAGAAGAGACCGATCACTTTCCCTGCCTCACCTTCTTCCTCATTTGGCCAATGTAAGGTGCTTGGAACTTTAGAGGGTTTCGGGTGGGTGTAGGATGGAGAGAAGTTAGGCTCAAAGAAGCAAGGGTTGGAGACTGCGTGGCTGGGGAAGGGGTTCAGGAGGTCAGATAGCTTTGGGAGCCTCTGAAGGGAGAGGATGGGAGCTTCACCGTTGAGAAAGTCACGCTGGCTTTCCAGGATCTGGGCCACTTGCTTTATCCAGGCTTGACTGACAGCAGGGTCTGAAGCCTGCAGAACGTAGCGCTGGATCCCACCTTCTGGCCCTCTAGAGGTCAGCGCAAACCGGCAAGGGTTGCCTTGGAGATTCCCCTCCAGCCCTAGGCAGCTCACCTGGATTGGCAATCAGAGACAGCTCTCAGATTCTGCAAGCCCTTCCACCAGCCCCAAAAGCCTGAGCCTCGCGCCATCCGGTCCGCTCCCTCCATATGCCCCGCCCCATCGGGGCTGCTTTCTCCATACTGCCCCGCCCCACCTTCCCCCTCGGTCGGCTCCCTCTATATGCCCCGCCCCACCTTGATGCTGTTCTTGTACACGTAGCCAGGCTGCGCGCCACCTCGGCCTCCTCCTCCCAGGGCCTCACTGAAGATGATGATTTGCTCGAACAGGAAGACACGCCTCTCCCGACCCCGGGAAGAAAGCAGACCCCCAGCTTCAGGCTCTGTAACCAAGAATGTGTCCTGGCCCAAGAGCTTCCCCTGAGCAGTCAGTTTTCCCTGGAACCGAAGGACATGGCCTCTGAGAAGGCGCAACAAGAGTTTGAAAAGAGAATCATCAGCTTCTCTCCCCTCCCCCACGCCAACACGCCCAAGGAGGTAATTCCTCATAAGGATCCAGACTACTAAGACCTGAGCTCAAGCGCAGTTCACTGCTCTGCCCCGCCCCATTCCCGTACCTCAAATCCCCGAAGTCTCCCCAGGGACATCATATCATTGCATCGCTTAGGTACGAAACACATGACCTCCACAGCTTGCTGGGACACAGGTGAAGAGAGGCGTCATTGGACAGATTCGTTACTGGCAGGGAAGCTCCTTTTGAGAGAGCCTCTCCAAGAATCCCCACCCCGGGGGCCTCTCCCTGAAAGCAGACATCCCTGAACCACCCTGAGACTGTGGTACCCACAAGATGAAGAGGATGAGGCAGATACAGTGTCCTGGGGACCTCACCTCCAGCTCTTCAGTATCCTTCCCAGCTCTTCTGTAATACTTTAGAAAATCCTATAACATAAAGAAGAAAGCTCTGTAGAGCCCGTCCCCAGCCAGCTGTATCCCCAGGGAGAAGGGTGATCCTGTTCCCAGCTCTGCCCCCCAGAGACAAGGGTAGTTTTGCCCCCATGGAGAAGGGTGGTCCTGTCCCCAGCCATGTCCCCAGGGACTAAGGTGGTCCTGACCTTCAGCAGCAACTGGTATTTCATGATTCGCTGCACTGGTTTAATGAGGAGGTCATTGAGCTGCAGTCGGTGTCCCAGCTGCTGCCTCAGCTCCTGGGGACAGGGACAGGGACAGGGACAGGGCAGTCATGTAGCCTGAAAAGTCTATTCACGCTGATCCTGTTCTTCTTTCCCCCAAGGCACCTCAGCCACTGACCTCAAAGTAGCTGTCCCCAAACTCTGACAGCACATGCTCTGACTTGGGTTTATTCTGGCAGTACACCACATACATATGCAGTCGGCGCTCCTAGCAGGGATCACGTTGAAGAAAGTGGGGGTGGGTGGTGACAAAAGAGAGCCCATCTCCACAGAACCCAGACTCACCCCAAGCCCTTCTTCCCCTGTTAAGGCCCAGCCTCACATGTTTGATGAACAGCTGAGCCAACCAATCGGGATCTTTCAAACACTGCTGCAGCTCCTGCAGGAAGTAGCTGGAAGAGTTGTGAGAGGGAGAGAAGCACCTCACACACTCCTCAGAGCGCTCACCTCCAAACGTCCCAGACTTTCAGCTACACGCCCTAGTCGCTCTCCCTACAAAACCCCAGTGGCTCCTTGAATCACATCGGACCCATGGTTCCAATACGGAGTCTAGGATAGACTACCAGAGAGCGCTCTTCCCCTGCGCCTGGCTCAGGGCTCCATCACTCACTCTCGGTGCCACTCATAGATCTGCTGAATGTTCCCAAACACAATCCTGTCACGACCTCGGAGACTCTCAGGTACCCCCTGAGTAGCCATGGTAGCCATGTAACCCTGTGGGAAGGACGAGATGGGACTATGATTACGTAAGCTCAGCCTCAGGTCGGCCCTGCACAAAGTCCAGAAGTAGAGGGAATCTGTAAAGTGACCAGAGGGAGCGACCTATCTGAAAAATATCCCCAGGCAGAGGGAAAGGGTCAGTGGGCCACGGCTCGGGAGGAGAAGCGAGAACTACCTCTACGATCTGTCCCAAGTCGTCCACATATGTTCTTTCTGTTTCCACCAGCTCACCCAGGACAAACCTAGAGAGCACAGCATGCCTTCACTTTAGAGTGTGGCTACATCCTTAGTTTAGCCTGTTCTTCTTGCACTAAAAGAACATCACCACAGCCCTCTCAGAGGGCAGTAAAATGTGAGGGATCTTGGGGAAGTTCAACCGAAGTGCCCTGTGAACTTACATACTCCTTTCCAGAGCCATCTTCTTCTGCTCTTCCTCATTCTTGGGAGCTTGGGACAAAGTTTCCTCTTCAGCTGGCTGGAAGGTAGGAAGTGGACAAACAGAGGATTGAATACCTTTCACTAAGAACCTTCTCAGATCTATTCTTTTGATGGTTTTAAGACCTGTTTGTAGCCGGGTTGTGGTGGGGCAGGCCTTTAATCCCAGCACTTGGGAGGCAGAGAGCAGGTGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGAACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAACAAAATAAAAAATTAAAAAAAAAAGGACTTGTTTGTAGGGTCTACATAGGTACATCATCTACATAGATAGAGTCTATGTAGTTCTGGCTGTCCTGGAACTTGCTGACCTTGAACCTACAGACATCCTCCTGCCTCTGCCTCTGGAGTGCTGGGATTGAAATTTATTATTATGATTATGATGATTATTACCATTTTTACATTATGGGGGGCCATGTGTAGATACCCA
U5_15 126624800 126639850 15051 TTACTATATATATTGTTTTGGACAGCCCCCCTCTCCAGCGCATGCACATACCCAACCCAGGGGGCAGTGAGGACAGCTCAGCGGGGCTATCTTTGGCCAGCAATTGGGAAGAGCTGCAATGTGAGTTCTTATAAAACCCTTTACAGGTAGGTAGATACCCCTCGCCCCACCCCCAGCCCACCATTCCTCTGCTCCCTTTTCGGAAGATATTCAGCCAGTGTGATCTGAGACACAAGGTTCTGGGCATCAGGCGATCACCAGAGAATTTTCCCTCCTGGCTAGCTGACTAAAGGAAAGAAATGGGTCCTCTGACCCTTCCTCAACTTCCTCAACTTGAATCAGCAGGAATCTAGAAGTGGAAAAGGACCTTAGATATTCTCTGTTAAATCATTTTTTTTCTGGAAAACACCCGTACATGCTCCAGCCCTAAAGCCCTAGGGCTAGAGTAAGCAGATGCCCTAACCCCAGCTCAAGCTCAATGCTGGCCCCCTCAGACCTCGCACTCACCACGTCCCCCCCGGGAGAAGCAGCAGCCGCATTTTGCCAGCACTTTTCGAATCACACGGGGACAGGCACAGCGACCCCCTTTGTGACCCCCCCGCATGGCGCCCGGGGCCCCCCGCACCCCCCACCCGGGCCGGCCATGCAGGGGGAATCACGGGGGGCGGGGCGGGGCGGGCGCAGGGCGCACACCCCCCTCCCTCCCTCCGGGGTCCAGGCTTGGAGAGGGGGGGAGAAAAAGGATGGGAGGCGGGGGTCCAGGAGTAGACGGAAGTCGAGGCAGGGGAGTCTGGAGGGAGGGCCGTGCGGGGCTGGGCCGCGGAGTTGGGCGGGGAGGGTTTCCGGAACCCCGGCGGTGCGGGGCCCAGGGTCCAGATGTCCAGCCAGAGGAAGGGGTGATGGGGGAGGCCCGGCCGGGATGCGACAGCCGCTCCAGCTCCGTCTCCGGCCTCGGCTTAGGGGTGGCTCTAGGCCCGGGCAGGGAGGTGCGGGGGCCGATTCCCAGAGCCACTGGAGCCGCGCGGGTCCGGGCCGAGCGGCGAGCGGAATAACAGGCCGGGCCACGGTGCTGCCGTGGGGCGGGGCCTCTGCGGCCAGGCTCCGCCCCTCCGCGCTTCCCCAGCCCCCTCCCCCAGGCACCTCCACGCTTGCAGTCCGGGTCCCCAGACCTCCGCCCCTCCTCCCAGGCCTGGGCTCCCTCTAGCATCCCAGCTGCAACTGCGGGGCTCTGGGTTGGGTGGGCGTGAAGGCTGGAGGTGGGTCTGCCTTGCTCGCCCCTTGGACTCTAAGATGAAGAATTCGCAGGATGGGGCCTTGTTGTGTGTGAATGGTTCTGAGGTTCTGGCTGTCTCCCCCGCCACCCCTCACCCCCATGCCCCGAGTAGGGCTTGTGTGCCTGGGGAACACTTTGTAGGGAACACAGCCCAGGTGCCGCCCTCTCCCCCGACCCATCTTTCCTCCAGGCCCCAGAACTCCAGCTAAAACTAATCACCAATTAAGGCGAAGTCCGGGCGCCTGGGCTGGCCCAGGGCCAGGGGTGGGGCGCGGGGACACTCCACTCCGCCTTCGGGGGCTCGGGACCCGCCGAAGTGAGGACACACATCGGGCTAGAAGAGAAGCGTGGTGTCTGCGATTTAATGGTCCCATGCAAATTAGGCGCAAGCCTTTATGCTAATATGAACAGAATCTATCTGAAGTCACCACTTTGCGCAGGATGGCAAATTGTGCCCCACCCAAGCCCAGCTTCTTACCGGGCTGCGGAGCCCAGGCGAGGACCTCAGGTTCGCGCTGCCCTGTCTGATCCTCCTCGATACAATCACTCTCTGTCTTGGTACTCCCAGGCCCTAAGGGATCTCTCTCCGGGGGCTGCCGAGACAGAGTCACTGTAAGGACCCTTTCCTAGGCTGCCACGACCTCCCCCTTCTTCTCTCCCTCCTGTCAAAATACACATTACTCTGAAAGCGTCAACCAGAACTTGGCATATGGCCCTCGTAACTGAATTTATGGATCCTTGCGACTGCTCCATATAACTTTTGTTTGCGGGGAAACTGAGTCGTGGAGTGCAATTCTCCTAAAGTCCTCTAAAGACCCCCCCGACCCCACCCGCGGTCCCTCCTCCTTACCCCCTCCTGCCCGGGTACGGCGCATGCGCGCAGCATGCCACCCATGACCCCCAGTATCCGGTCAGTGCGGCCAGGGGTGGGGCGGTCCGGGGGCTTCATGCTGGACCCCTACCCTGCTTCAAGTTTCAGGGCCGAGGGGCCACGGAAGCTGGGGGGCTCTGAGGCCTGCGTGCGTCCGGGGAGTTGCTACTGCCGAATCCGCCCCCCTGCCCGGCTCCGCCCCGGCTCCCACCGCAGCCAAACTTCTCAAAAGGGGTAGGGTTGTTCCCTTCTCCACAAACGTAGCTGAACCACAAACAAACCCCTCTTAATCCTGATTCCATCAATCCAAGTGTGGCCTAGGGCTTTGCAGGGTTATTGCAACGCCCAGCGTTAGCTCTGCCAGAGGAGAGTGACTCCTAGGAAGAGAAAGGGTCCATTCAGGCATGCAAGGAATCCCCATTACCCCATCTCCTCAGCGGGTCCACACCCCACCCCCACCCTGTCCGAGGAACAGAAGACGGGTGTGGCATAGAGTTGCATATTTCACTTTATTTTTATTAAATTAAAAGCTACAGTCTGGCAGCAATTCCAGATTTGGGGGGGGGGGGCGGTGAGGAGGCTCCTTATAGGGGCAGAGAAGAGCGAGAGACAGACTGACAAAGACAGACACGGGAGAGAAAGGGTCCGTTAAGGTAGGACTACTGTTATCTTAACACACAGCTGGCTGGGGGAGGCAGCTAATATCAGTCCAAGGTGAGACAGTATTCAGGGGCTGGAGTTCCAGAGACCCCATCCTTCCTACCCAGGTCCTTTCTCAGCTTAGTCACTGGAGCACGGTACATACCAGGAAAAGCCAAGGACACTGGAGAAGCAGGGGCAGAGAAAATATGTACACGGAGTGAGGGGACGGGAGCCTTGGAAAACAGCCTCTGCTGGAGCACTTGGCCAGTGGGGGGGCAGGGGAAGGGACCATGTCCTCTTCCCCTCTGAGTTCCCACCCCAGTCAGACATATCCCCTCAAGGATGGTGTGGGGGCAGAGAGAGGCGGGGGGAGCAGAGGCTTCCGTTGGATCCTAGTAGAGGACAGCGGGGGGCCTTGGCAAAGGAGCTGTCCCTCAGTCGTCTGTGATACCCTTGGCTCTCAGTTCCTCTTGCACCCTAGTCAGGTAGGTTGGGTCCGGATACCCAAACCTGGGGCAAAGAGAAATGAGGATCAGAGTTAGTCGTTTGGACTTGATCGTCCTTACCCACCCTCATTTTGAGTGGCTCAGTTGGGGCCAAATTCAGACCCCTCCCACTTCCCATGAGGAGGGACCTCCAAAACCAAGATAACCCAGCCAGAATATGCTTTTTGGGTGGGGAGGGGAGGTGTTAAGCTGAACGCGTCAGGACCAGGATGAAAACAGACTGAGAGGGTGGATGGCTCCCCCAAATTAGAAATGGTGAAAGGGAGACGCCATGGAGAGGGAGATGAGGAGGAGTCGCACAGCTGGGGTCCCCCTGTGCAGCTGGTCTTGTGGTGGATGTCGTTCCAAGTGATGACATTGGGTCTCCCTGTAGTCATGGACGTGCCGATGGTGAAGGTGAGGCGCTGGTCAAATGCCTTTCGGAACAGGGTCAGCACCTTGTTGCCCTCGGGGCAGTCCGGGAGGTAGGCCACCCGTGTGGTACCAGGGTACCGAACTCCTGGGTTGGGGTGTTCAGCCTGAGAGGAAGAGGAGGCAGAGAGCAGCTGGCTGTCTCAAGGGCCTGGACACCAGCGAGGGGTCTGTCAGTCTGCCCTGCCTTTTGTCCGTGCATTCCCAGCAGACTGACAGAGAGACTTCCAGGGACCGGATGACTTTGGAGCCTGGGCAGGAAGAGGCTGTTTCTAGACTCAATCCTTATCTTTAATCCCTCACATCTCAAGTCAGGGACAAATGGACGTTCAGAAGTGATGGCAGTTACTTTCCAGATAAGTCAGCACAGCTGCAACAACTAAGAAAGATTGTATCTTTTCCCATTCCCTCCTTTTCCTAAAATACTACTGCCTCTGCCTCTTGTCTCCAATTTCTTCTTCTTCAGGGTTTCTCTGTTCCTGTGAATCAACTGGACATGACTCTTTGTCCAGCCGGAAGACATCTGCCTTCAGCTCTTATTGTCATATAGTTTGCAACAGAGCAGCACTCCCAGTGGTTAGCGCAGCCAGGTTCCCTTCCTGTTGTGCTGTGCCTTCCTTCCTTCACCTGGGCTTTGCCTCCACAAAGAGTCTGACACATCCACTCAAGTCAGGGTACTAGTGGGCAACCCTTTCTCTCATGACTGGTTTCAGGTTTTTTTTTTTGTTGTTTTTTTTTGTTTTTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTGAGACAGGGTTTCTCTGTGTATCACTGGCTGTTCTGGAACTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCACCTGCTTCTTCCTCTCAAGTGCTGGGATTAAAGGTGTGCACCACCACCACTGCCTGGCCACACCTGAGTTTTACATCTGCCTGTGGGTCATGCCAATAAAACAGTGGGGCCTCAAGGAGATGGCCCCTCCCCAACAGCTCCAGAACATAAGTCCTAAGGCATGCTGGGAGACAGCAAGAGTCTAACATGTCTAACAACATACTCTCTACATTGCCTTTTTTTCTTAAGTCAAGAGACAGTGAACTTGGAGGAAGCCAACGGGTAGGAACTGTGATCTCTTACCCCCTGGACGCCAGGCGGGAAGACATATTGGATGACAATGGTGCCATACTTCTCGTAGCTGGGCAGCAGGAGGGTGGCATCCTTGGAGACCAGCATCCGTCCGTTCTGGGGCTGGTTGCCCACAAGCTGCCCGTAGAATCGGCCGCACATGGGGCAGGCCTTTTTCACCTGCAGGGCCCGTGTGATGCAGCCCTCACAGAAGGAGTGCCTGCACTTCTCCAGCGTCTTGGCATTCTGTATCTCCCCCAGGCAAATGGGGCAGGTGGTTTCCTGCTCTTCAGCGTCCTCTCTGAGACGAGGAGGGAGTGGAGGGGGCAGGGGAGGAGGAGGAGGGGGTAGCCCTCTTGCCCCTGGGGGCAGGAGTGGGGCTGCTCGGAGGGGAGGAGGGCCTGGGCGGTGCAGCTCGGGGTGTTCCCCTCCACCCCCCAAAGCCAGGCAGCCCATGAGCTCCCCCTGCCTCTGGGCTTTCTTTAGCTCTTTCTCTGCCTCTTTCAGTAGCCCCTTGAGAGCCTTCCTGGCCAGGTAGAGCCCATTGGGAGGGGCTGGGGGAGGACCCTGTGGGGACAGCTGGAGCACGTAGATGTCAGAAGTCTCGCCATCTATGAGGATGGACACCCGATGCTCCTCCCGAAGCCGGGCCAGCCGGGCTGGGGTCTCCTTGCTCAGGAAGTCCCACACAGGCTTGGAGACAGTCACTTTGTTCTTGCAGGAGCCTCCACAGGCTGCCATTCTGGACAGGACGAACGACACTGCCCCCAGGGTGAAAGGGACTCAGAGTGGGGAGTCCCCATTTGACAGATAAATGTGCCTCTTACTTCAGGCTCCTTCTGAACCCCCTGTGTTACCTCTGTTTCCCTCAAAGCCAACCCCCAACACTGCCCACAAACCGGTTGGTTTTCTATCTCCACTTCTGTCCCATAGCCCAGCACCCTCTGGGCACAGGTTTCCATATTTCATCGTTATCTAAGCCAAGCACCAGCCCTGCTTGCTTCTTTCATTTCTCCCCTGAGCACAACAACCTGCCCCTTCTCCCCAAGATGGAAAAAAAAAAAGGTTCCTCCAAGGGAAGGAGGCTCCCCACCCCTACCCCACCCCATCCCACCTCCGTTTGCTTTGCTCACTTTGATCCGGAGGAGCTTAGAATTGGCATGCAGGCTCACTCTCCTTTCCCCCTGATACCTGGAGATCCTGTGGCCTGGTGGAATGGGAAAGAAAAGAAAGTAAGGGGGCTGGAGAGATAGCTCGGTGGTTAAGAGCACTGACTACACTTCTAGAGGTCCTGAGTTCAAATCCCAGCAACTACATGGTGGCTCACAACCATCTGTAATGGGATCTGATGCCCTCTTCTGGTGTGTCTGAAGACAACAAAAGTGTACTTATAATAAATAAATAAATAAATAGATAGATAGATAGATAGATAGATAAATAAATCTTTAAAAAAAAAAAAAGCTGGGCAGTGGTGGCGCTTGCCTTTAATCCCAGCATTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTTGAGGCCAGCCTGGTCTACCAAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCAAAAAAAAAAAAAAAAAAGAAAAGAAAAGAAAAGAAAAAAAACAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGGAAAACTATTCCCCAACCCCAAAGCCCCAACTCAGGAGCCCTTGAAATGGCCAGGAACCTGGGGTAGGGGTACTGGAAATGGGGATTTCTTGGAATACAGGAGGAACCCGAAAGCTGAGGTTCTCCTGGGGCACGGGTACCTGAAAAGACCACAGAGACGAAGGTAGCAGAAGTCCCTCCACCTCCACCTTCAGCTGGCCAGAGCTGGAGACGAGGAAGGTAAAGAGTTATGATAGCATTTCCTCAGCCAGCCCAGACAGAAAACGCTCTTCTAGTTCCTCTCCTCCCCTGCAGAATCCTCTCCAAGTTGCTCAAGGAACTTCATCAGGCTAATTCCTCTTTTCCCCCCAGTGCAGAGGGAGATCCTAGATGCAGATAAAGACAGGAAGGAGAAAAAAAAAAAGGGCCTCACAGGATGAAGGTCGTACACGCCACCTCCCTCAGATTACACTGACAGAAATATCTCCTAAACTCCTCGCTTTACGTTCTCGAATAGTTCAAGTCCCTGTTGTTGACAGAGACCTTTCCTTGAGAGCCGGAAGTCTGTTAGGGATGCCAGCAAAGTGTTAGCAGTCCCGCAATAACAAAGTTCTCATTGTCACCTTTAAACCCAGTGCCCGCAGAGCTGCTGTCAGCTGCAAATCAAGCAGGAGCTTGGCTAGAGGGACCCGAGGTTTTCCCCTGAGCCCTGTCACAGCCCAGCTCCAGGGCCTTTCCCACAACACAAAGTGCAGCTCAATTAGGAAGAGGGGCCATTCATGTGACCTCAATTCCTTGTGATCCAAAAAGAAGCCTCTGTCCGTTTGTGCCTCCATCATAGGGTCCTTGAGATGAAAACCTAAGCATCTCAACCCGCATGGTCTGTGCACGGTGAAATGCCCCCTCCCCACCAGAGAGAACCCAGGATCTAGGTGTCCTAGCTCCAAGTCCTGCTGGCACGGCACAGGGAGGCGGGGCCACAGTTTAGTCTGGGTCTAGCATTCCTCTGGGGCAAACCAGGCCGGACACCAGACCAGCCTCTTCCTCCAGACAGCGGCCGCTGGCAGGGACGGCGAGAAAGGGCAAGGGTCCCCTGGGGAGGTGTCCACTGAGGCACCCCCCACCCTTCTCCCCGAGACTGCCCCCGGATCCATCCAACCCGAAACCAACTCCCGCATCCCTACTCGAGGAAGCCCCGGAGTCCCGCCGCTTGGTGAGGGTGTGTGTCCACCCGACTGCGGCCCTCAGAACCCCCAAATGGAGGCCAGGGCTGAAGGTGCGGGAAAGATGATGGGCCCGGGCCCCGAGGGCGCCCTCTTCTCCCGCGGCGCGCGCGTGGGGGTCCAATGGCGGAGGAGGGGGCGGAGGGAAGCGGGGGGCTTGTGAGGGTGCGGACCCGGCCTGGGGGGGCACGCGGTGGGTGTGGGGGCGCCGGGCTCACCTACCTCTCGTCAGCGCCGCCATTGCCGGTCACATGACTGAGGCTGTTGCTGGGATGACGGTCCGGGCCTTAGCAACAAGGGGCGGGGGGGTGGGGGGGAGACCCTGAGAAGGTGGAAGGGGATGCCGGGGTGCAGACCGAGAGAAAGGAAGGGAGAGCACCCCTCCCCTTGGTGGGTGATAATCACCGTTTCCTTTGACCCCCTCCTACCCGGGAGCCCCTGCCTCTGCCCCAAAACAAAAATGGAGGCGCAGTTCCAGTCTTTGGAGGGAGGGGTGGCGTCATGTAGCCGAGGGTAGGAGGTTAAATCCCTTAGAAACGCCACTCTCTTGGCTTCCCCCTCCCGGAGGAGACCTCTCCCCACTTCTTTCCCAGCCAACTGCGTTGCACTCTTAGGAACTGGTAGGGGTGGGAGCAGTGTCGGTGGGTATATAGATTGAAGGGGAGGGAAATTTTTTTGAAGTCTTACGCTGGAATATGTTGGATGGGAAGTTTAAAAAAAAAAAAGGAAAACATTGGTACTACAAAATAAACCCTGTGACTAAACTACTTCAATCCCCTCCCTCAACTCTTGCCTCCTTCCAGACTGTTACCTCCGGTGACCCCCCCCACCCCCAACCCTCGCAGCCTGTTACCAGAAGTGGGCTGGTGACTAGTTTTCCACAAGTAAGGACACTGGTCTGTGCAGAACGTGAGGTGGATAGATCCTTTAAAAATGGCTCAGGGTGGTGTTAGTGTCAAGTACATGGCTCAAATAGAGTTAAAAATTCCCAAAGAAAAAAAGCTCTGCTCCGCTCTGCTTCCCGTCAGAGCTGTCTCCGCCTCCTCCAGCTCACTTCCCTGGGGCTACATCAAGCCAGGTTTTTCCACCAGCCAAGAGACCTCCTCAGCCAAGAGAAGAGGTACTGACTCTCCCAGACTAGCACCAACCACGTCATCCACCCCTCTCCATCCCCTCCCCCCTGAACTGGAGGACTGGTCACTCCTTCACTTTCCCAGGACCCTGCTGGACTCTTCCTGGCCTCTAAGCAGATAGCTTTCCCAGGAAGTCTGCGTGAACAGAGAATTCCTTCCCACAGAGCTGGTACCCCAAGAGGCCCACTAAAGTTAACAGATGGAGGAGCAAGAGCATAATGAAGACAGACACACCATCCCACTCCCACTCTGGCCGCCACAAGGTTGGCGACTATTTTCCCGCTCCCATGTCCACCCCACAACTCCAAGTCCACAGTCATCTTAGAACACCCCCCCCCCACACACACACACACATACTCAGGAGCCCAACACCTTGAAGACCAATATTTTTCAGTTGGACTCTGTAACTCTTAAACATAGGATGGGTTACTCAGTTTTTTAAAAAACAAAAACTTCTGTGAATGACTTAGATCAAATAATTTATTTTATTGTGATATCGGGAGTAAATATACATATTTTATTAACAAAACAGATGGAAAACGGAAGCAGTAAGTGAAGATGGCAACACTGCCCACGACCACAGCCGCACGGTGAGACCCCAGTTGTTAAGGGACCGGAGTCACTTATTGCATTCAACAGGGGAAAGGTTGCTTGAGGGATGGGAGACTCAGCAGGTCCCACCCACCCCAACCGGGCACCCATCTTGCTGCTTCCTTTGGTGAGGGAGGTGGGCTTCTCCCTGAGGACCTCATGGACACACAGCAAAGCAGCTCCTTGCCTTCAATGCCCACCCCACCTCCAGAGCGCCAAACAGCGAAGATGAGTGGCTTGCTTCCAAAGGCTTTAGGCAGAGACAAAGACAATCTATCTCTCCTTAGAATCATTTTCCCCAAGATGGTCTCCTGTTTCCTTGGCTGGCCTCAACCTCGTCAAGTAACTAACTGGCGATGACCTTGAATGCCCCATCCTGCTTACCACTACTTCCCAGGGCCAGGAGTGCAGGCGTGCACCGCCACGCCTGGCTTAAGTGGTGCTGGGGGTGGATCCCAGAGCTTTGTACATGCTAGGCAAGCACACTACCAACTCAGCTATGATCTCCAGCGCTCAAACCCAAACCTATTTGGAGTGTTCCTGAGACCAGGTCTCACTCAGTAGCCCAGGCTGGACTTGAACTCTCAGAGCCTCCTGAGTGCTGGCTTTACAGGCACGAGCTGGGAGAGCTAAGATGCCTAGCAGGTCCTCTCCATTCTGAGGATGGCGTGCATTAATATTCAGCTGTCTTTGGAGAGGACTGGGTGGATTGAGTTATGGCTCTGACTCCTCTCTGGGGGCGGAGAGGGGAGATGACAAAGAAGGCCATCTGTCCCCTGGGAGACACATTAGCAGTGGAAGATGGGACAGAATAACAACTCCAACCAGAAAGGGTTTTGAGAAGGAGGGGGAGTTCTAGGTACACATGCCCCAGGGATGCGGAGGCTCCTTCACTCGAGAGCTCTATCCTCCCCAAATTATGGAGATGGTACTGTTCCCCTCTGCTAGAAAGAAGCCCAGGAGTAGTGAGACAGGAAGAAAGGGTGTCACCCAGGCAATGAACCCTGCACTCCGACCTGTCCCGGCCTCTACTGCTCTCCCTGCTCACCAGCAAGGCACAGGGTTGTGGGAAGAGCAGCCCGAGTCTCACACCTGCACCTAAGCTCGGGCACCGCTAGCCTCTGGGCTCCTGCACCCAAAGTGCTCACAGAAAGAGGTGGACGTGGGGTGGGGTGGGGTGGGAGAACGGGAACAGTCGGGAACAGTCGTTGCTCCACAGGGACCTTCTCCATCATCTGCATAGTCACAAAAGTCCGCTTAGGCTTAGGTCCTGCCTTGTGAGGTGCCAGCCTGCTGACCACTCCCCACCTGACCTCCAGGCCAGACTGTCCATGTGTGAATGCCTGCACCCACATCTGGAGCATCCTGAAAGCCCTCCATTCCAGGGCTTACCAGGGAGGAAGCATGCCACAAAGTGCCCCATAAAAGTATCAGCAAAATCCAGACATTCAGTTTCTTCCACTCAGCAAAAGTGCATCCAGGCAGTTGCTTGAGCCAAGTCCACTGTTCAGTCAGGTCCCGAAGATGGCAAACAACCCCTCCCCCAGCTCCTCCTATTTCATAGAAACAAGATATTTTTCAAAGATCAAGGCACTTAGAAGAGTCGACAATAACACTCTAAAGAAGCAGGAGGGCTTACTGGGAGCAAGGAGGGAGGGCAGAGAAATGGACAAGGGGAGAAAGGACAGTGTATGTCTGGGGTGGGCGAGAAAACCCTATCCACCAGAAGGAAGTTATTTGGATGGAAGGAGCCTGCAGCCTGAATTTAGCAAAGAAGAGAAGTAACGGCCAAGTCCAGCTCCCCTAAGCCTCTCAGAACCCGCCACCTCCAACATAAAGCCGCAATCACCCTGGAGTCAGGCAGCCTCTTAGGCAAAGATGTTGGCAATAAAGTCCAGGAATCGCTTAGCATACTGCTCAGGATGGACAGTGGAGATCTCTGCCCCCGCCTGTGAGAAGAAAAAACAGGGAACAGTGTCACTCACAGGAGCTCTCCGCCTCCCCCTCAGTTCACCCTCCTACTGCCTTCCCTCAGCCATCCCACTACTCTGACCCCAGAAGCGTTCTTTCCAGAGAACAGTCAGGCCAGGAGAGGAAAGGCTGTGGGGAACCCTCACCCCGTGCTTGACAGTCTTGGCTGCATGAGCTGCTTTCTTCTTGGCATCATACTGTGTCAGAATGTCAATGAGGCCCATGAAATACACCTCCTTCTGGGGGGCCCCTGGGGAGAGAGGCCAGCAGTGAGGACAGGGTACAACAGTCCCAACTCCTTTTGCCCAAGAGATAACTTTGTTGTGAAAAGCTAAGCGGTTTCTCCACCCACCACCCTGGTCTCTGTCTCCCAAGGGTCCCCATCACCCCGCTCCCCCTAACATTTCCAGGTTCTCTCTCACCCTCCGCACTCCGGATAGCATAGACATCGATGAAGGACTCAAACTCTCCTGGGCCCAGTGGCCGGTGGGAATGGATGTAGCCTCCGATACCCTCAGGGGAGGTACCATAGGAGCCCACCAGGGCGGGAGGTCCAGCCAGGTTACAGTCCCCATCCCACTCAGACTCCTCCTCCCTCACAGGCCCCTCTTCCTCGGGTTCAGAGCCCCGGATGATGTCGTGGATGCCCAACAGGAGGCTGTAGTCCATGATCTTCAGCTGCACTAGAAACTGAGACCCACAGACAAGTCAGAATCCACATCTCCCCCAACGCCAGGCACCACCCACACATCTCCCTTCTTGGTTTCAGGGCCCCCTAGGGAAAGTATAGAAATGGGTCATGGCGGACCAGTGCCCAAGTCCTAGAACCTAAAGCACCCAGCCCCACACAACCCCTAGCCACCGACAGGCCTAGCCTAGGGGGTTTCTCCTGGTTCATCCCCAACCCTCCATTCAGAAGCCCCCCACCCCCCAATCAAGGCAAGATAAACCTTATACCACAAATGGAAAAAAACGTTCTTCTTTGACTAGATAAGCGTTTCTGAGCACCCTGCTTACCTCCACATCTCGCTTCAGCTTCTCCAGGAATACTTTCTTTTCTTCTTCACCAATATACACTTTCTGGTTCTTGTTAAGAAAGTCCATATCCTTTAGTGTTGGCAGTTCTTTAACCTAGAATAGGAATACAGAAGTTTCGGGAATTTATCCCGTACCAAGAGACCCCCACCTCCATCCAGGGCTCTCCATCTCAGAAGGTCTAAAGAGAGCAGCAGCCCTACAGTTAGTTGCCTTATGCCTGGACTCACACAGTCTTCTGTGCCTCAGACTGCACCCCCTGCTGCCTGCTTACAGATGGCAGTCAATACCCTCCATACTGACTTTCCTTCCTTCTTCTTCAGTAAAAATAACTGCTGGAGCACAGCGCCCAGGACGAGGTATGGGAAATGAGCACGTGGCTCTGAGAGCCTGCCTGGTATTCCTGCTCATCTCCAGACCTTCAGCAGAGACCAGCCAGGCACACAGACGAGCCTATGGCGACGGCAGCTGTCCCTGCCATCCCAACATCAGTGGTTTATCATCTAGCTCCTTTTCAGTAGTCAGGTAAGAATAAACATCTGGCAATCGGTACCCAGTCTGGAGAACTGCTTCCTTATCCTAAGGAGACCCCTTTCATCCATCCCTATGCTTCAGTTACCACCTAAAAGGAATATTACCTTTTCCTTATCGCTGGCTTCCCGGGACACTAGAGAGCCCTGTGAAGAGACCAAACTCTAAGTGAGATGCAATGGCTAGAGGAACAGCCAACAGTTACTGAAGGAACTCTCTTTCAGGTTACCCAGTGCCCTGTCAAGGTTACCCCCATTCTCAGGCCATGAGCAGTCTTTCCTTTAGCCCAGGTTATCCCGTTCTTACCTTGAGGTCATACTTCCTATGCACAGGAAGACGATGACTAAACATATTGCGCATCACGAGCATGTAGCTGTCTTCATTTTCTACACTGACTCGGTACATGCCCAGGAACTGGGGCAGAAGCGTGTTGCCATGACATTTCACTATGTACTAGGGAAGCAAACGAAAGGGACAATTGGCATAGGGACAGGAATCCAGGTGCTTCCACCCCAGAGTCAGGCAAAAATCTCAAACAGAGGTTGCAAAGTCAAGAGGTCACACAGTTCAGAAACCTCGAGAATTCTGCCTGCAACTATTTCTATAAAAATACCTTTTCCCATTTCACCTTTTCATTTCTTTGTTTGCTGTCTGAAACTGAGTCTCACATAGCTGGGGTTGGTCTTGAACTCCTGATCTTCCTGTTTCTACTTTCGAAGTGTTACAATTACAGGAATGAGCCACTTTATCTGGCTACCTTTATAATTTTTTTCAAGGAAGGTAGGTATTATTATTCTTATTCCAGACTCTACACAAGTAAATCACAAAAGCAGGGTTAAACTTAGATTTACTTCTTGGTGGGTTAGGTTTTTGTACTATAAATGTCTACAAGGAGATCTATAATCAATTTGGATTGTTTTTTTCTTCAAAAGGTTTATTTGTTTATGTATATGAGCACTCTATTTGCATGTGTGCCTGCGTGACAGAAGAGGGCATAAGATCCCACTATAGTTGGGAGCCACCATGTGGGTGCTGGGAATTGAACTCAGGACCTCTGGAGGAGCAGCCAGTGCTCTTAACAGCTGAGCCATCTCTCCAGCCTTTGTTTTGCTCTTTTTTTTTTTTTTAAAGATAGGTTTCCCAGTGTAGCCCTGTCTATCCTGGAACTCACTCTGTAGACCAGGTTGGCTTTGAATTTACAAGATGCCCTGCCTCTACCTCCCAGGTGCTGGGACTAAAGGCATGTACCACCACTACCAAGCTATTTAAATTTCTCGGTGGTTTTGTTTTGTCTTGATTTTGGTTTGGTTTTTTGGGACAGGGTTTTTCTGTGTAGCCCTGTCCTGGAACTCACAGATCCCGTCTTAAACTCAGAGATGCTAGGATTAAAGGTATGTGCCACCACACCCAGCCAACACTTCTATTTAAAAATCTTTATTTATGTGTCTGTGTGAATGCCCCAGGAGAGGATCCCTTGGAACTAAAGTTCTAGGCCGTTGCAAGCTACCTGAGTCAGTCACCTAGAGACAGTGCTCTGGAGGAACAGTGCTCCTCACCACGGAGCCAACTCTTCAGCCCATGTCTGTTTTGTTTTCCGAGTCGGAGTCAGATGTTGCCAGACTGTCCTCTAATAAAGCTGACTTTAGACACTTGGAGTGGAGAGATAGCTCTTCCAGAGGACCCCGGTTCAGTTCCTAGCACCCATGTGGTGGCTCACAACCACATGTAACTCCAGTTGCAGGAAATCAAAAGGTCTTTTCTGACCTCTTCAGACACCAAGCATGCATATAATACACATTTGCCTATCATACAGGCAGGTAAAATACCCATACACATAAAATATAATAAAACATTAATAATTGTTAATTACTAAAAATAAAGCAAATAAACACTTGGTCTTCCCCACTCCGCAAGAAACTGGCTTGCAATGTGAATTTTCTTCTTTTTGACACAGGGTCTCAGGTAGCCCAGGCTGGTCTCTAACTCACTCTGTAGCTAAAGATGACCTAGAACTTCTCAAACTCCTGCTTCCCAGTCCTGAGTGCAGGAATTAGAGGCTTGG
D3_15 126608628 126623676 15049 CCCTCAGGAGCTTTTAACCGTTGCTCTCCCCACCCTCACCCCAGAATTCTTCCTGGATGGACTTGGTTTCCTTCGTGTTGGCTCCTGCTCTGATGTTGTTGTGGATCATGCATCGAAGGTGAAGCTGCCGTGGACAGCAAAAGACCCAGGAGCTGAAACTTATGCCCGTTACCGGTACCCGGGATCACTGGACCAAAGTCAAGTGGCCAAACATCGCCTACTCTTCTTCAAACACCGGCTACAGTGCATGACCGCCGAGTGACGTCTGATTTGGGCCTTCACACTGTCAGGCTGGGCCTGCCTCCTTGTTCCTTCCAGGATTATCCACCACCACCTCCATCCCTGTGCACCCCACTGATGAACACCCTGGCTTCCCGATCCTCTCCACCAATCTGATTCCTAACAGGGGCTTGTCCTGGTGACACCCTTCCTTTCTGTGAGCGACCAGAGGCCCGATGGAGCCATATCCTCTTCCCCCCACAGCCAGTGCCAAGTCCTCCCACAACCCCATTCCTATGGGGCAGGAAATGGGGAGGTTCACTTTCCAAGTGCCAAAGAGCCCAGAAGGACTCTAAGACCCTCAAGTGGAAACACTCTCACCTCCCGAGGTGGGCAGGGAAACTCCCAATTTGCAACCCCAGGGACATGCACCCCACCCCAGCTCTGGATCCAGCACCGTGTGTACCGGCTCCCACATACCCCCACAGAAAGCACTGTGACTGTAGTTCTGTGGGGCTGGTGAACACACGGTGAAAGCCATTTTTACTTGTGTCTCTGTGGTACTTTGGGTGTGGCGGGGCTTGTGGGGGGATGGGAGAGCTCCACGTGTAGATGCCGAGACCCACTCCACGGGCTTCTGGTATTCTCGGCTTCATTTTATTGGTTGCATTGGGGTGGGGGGAGCTCAAGGACCGTGGCCTTCCTCACACGCGGACTGTTGGGCCCTCTCACACTCTAAGTGCATGGTCAGACCCACACCGTGCAAATCTTTGGGCCAGTAGGTTTCTGGAGAAGAAAAGTGGACAGTTAGGGAATATTGGGTACCATATTCCCTGAGACCTACCAGTAATCTGGTAGCATGTCACCCCTTTGGAAACCTTAGTTCAGTTCTCCACCCTTGGTAAAGGGTGCCCCAGACTCTTTTTTTTTAATATTTATTTTTATTATTGTATATTATTAATATAATATATAATATTATTCTACTGTAGTTGACTTCAGTCACACCAGAGGATGGCATCAGATCTCATTATGGGTGGTTGTGAGCCACCATGTGGTTGCTGGGACTTGAACTGGGGACCTTTGGAAGAGCAGTCAGTGCTCTTACCCGCTGAGCCATCTCACCAGCCTGCCCCAGACTCTTGAACTTGGCTAGGTTGTTTGTTTGTTTGTTTGTTTGTTTGTTTGTTGGATGGATGGATAGATTGGTTCATTTGGGTTTTGAGGTAGGGTCTCTCTATGTAGCTCTGACTGCCCTAGACCAGGCTGGTCTCAGACTCATAGAAATGCTCCTGCCTCTGCCTCCCATGTGCTGGGGGATCACAGATGTGCCTGCCCCTGCCCTCCAATCTACTAGGCAGCTTCTCTCTACTTCCCCCTTGATCTTCCTCAGTCCCTTCTCTGAATCCCTGTCCCCAGGTCCTCATCTATTCCATACTCTAGCCCAGCACCTCCTGGCTCCCACTCTTGATGCTCCTCCCCCTTACCAGCCTCTCCAGTGCATGTGCAGCAGCATCCTGGACACTCACAAAGAGTTGTTCTGGAGACACGCTATCCAGGAGTCTCGCCCGGGTCAAGGTCTCGAGCACCAAGGCTGTGTGGGAAAAGTGTCAGGGGCTGGGGCCTCTACATCCACCCTACAAGTGTCCAGTCAGTCCTCCACCTCCCAACCCTCTATTCTTCACTCCACGAACCATTACACTGAGCCAAGAGAAGGTAGATCCCATCGTCCTGGCACCGCCTGGTGAGCTGCAGGAATCAAAGAGGGGTTAAACCCAAATCGAGGGTGCAAGAGGGATGACTGGGAGCTTTTCCTGAGTTTGACCACTCCCTCACCTGGACCACTTCCCGGGCCCCTGCAGCATCTACAAAAGTGATACCACTGAAGTCTAGGATCGCCACTCTGACAGGCTCAGCACCTAAGGAGGGAGGAAGCAGATGTGCCAGCCAACTCCGTGGTTCATGACCGAATCTCTCTCCCTCTGTCAGCCCACCTCACCTTTGTCAGGTACACTACCTGGCTGTAAACAAAGACAACAAGATCACATAAGTGCCATCCTCAGAACCCACTGGCCTCCCAAACCCAGAGGGTCCACGTTCCTCCTCCACCAAAATCCTCACCTTGATTCTTTCTCCAAGCCCCAGATGCCACTCCAAGATTCGGTGGAACTGCCCACGGGTTGCAAAGTAGAGGGGTGCTGGATAACTCAGGATACAAAGACCTGGAACTTGGAGGAGCTAAGGGGTAAGACTGTTAGAAACGGCCTGTCTGATCACTCCTGCCTTCTTAGGCTATATACAGCCCACCCACCTTACGGCTCTCTCTGATTGGCCTGTAGAGCTCCGTCCCCTCAGCCAATCCAAGCTCCAGGCACTGCACCCTAGGAAAGAAGCTCAAGTCAGAGTCCCAGCCTCAGCCTGGCCTGGTCTAGCCTGGCTGTAGTCACCACAGGGTACCTATCCCCCTTCCCTCATTTGGTGCCCATCTCTGCTCACCTCTGAGTTCTGCAGACCACTGTCATCATGGAGACGACCACACCCACAGCCAGGCCCAGGTCCACATTCAGGGTCACGACAGCCACCCACGTGACTATCCACACAGCCTGAGGGACATGGATATGGGTGGATTCTCCAGAAGGAACGGAGGTTCAAGGACAAATATGAGCAAGGGAAAGGAAGCGTGGTGGACAGACGAGTTCACTTAAGCCTACACACCAAATATAACACCAGACCCTGGGCAACCCGGGAGTCTGGATTGGGGGTGTAGTTCAGTGGTAGGGAACTCTCCTAATATTTATGAGGTTCTGGGTTCAATCTTCAGAACCCACTCCAAAATACCTCCAAACAAACACGCAGGCATGGCGAATCACATCCCACCCTCTCCTTTCCCCCAATGTATGTATGAGGTCTGTATGTTGGAGGTTGCACGGATTAAAGAATTTAGGGTGGTCAACTTTGGTCCATGGCAAAACCGGGGCGCTTGGGGGGTTAGCAACCTGGCATATTGCCATTTCTCACAAAGTCCACATGGCTGATGTGCCAGAGCTGCGGAAGTTCTTGCATCTGGAAGAACATCTGGCGCATGCTGGAGATATTGATGCAAGCCAGGACAGCCTTGAGATGGAGAAGATAGGCCTTTGTCAGGACAAAGGTTCCACTCACACACGCAGCGAGCGTGCACCTCTTTCCCACCCTACCTTGGGCAGGTAGTAGAAGAAGGGCCTCAGCCACAGCAGCGCTGCCAGGACCACTGCGCAGGAGAAGAGGCCTGCCAGCTGCAGGAAGGAAAGACAGAGACAAGACAGGGAGACTCCTCCACCCCGACCCGAGTGGGAGCTTCTCTCCCTGGCCGACTAGCCTGTCTCATCTTCTTCAAAACTCTCCCTCCTCCCCCAGAAAAGTCTCCTTCCTCCCAAAACTCCATCAACACAGAAAATGCCCTTCTCACCCCGTTCTTGATGTTCTGCCAATCACACTTAACCCCTCCCCCCAGCTAAATGTCTATCACTTGCCAGTCCCTCCCAGATGTGCACAGTTCAACCTCCCCATTCTTATAGCCCATCGCCATCTGCAAAGGTCTTTTGGTACTGATGCTTTGAGAAGCAGGCTAGCCATCTGTGTAGGATATAGCAGGGAAAACAAAACATATAAAGGTAGGAGTGGTATGTAACCATTCTTTCTTTTTGTTTACTGTTGTGGTTTGGTTGTTTTGGTTTTTGGGTTTTTAAAAGGTTTATTTATTTTATGTACATGACTACACTGTCGCTGTCTTCAGACACACCAGAAGAGGGCATCTGGCAGGGAAATGGTGGTGTACACCTTTTATCCCAACACTTGGGAGGCAGAGGCAGGTGGATTTCTGAGTTCAAAGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACATCCAGAGCTACACAGAGAAACCCTGTCTCGAAACAAACAAATAAACAAACAAAGAGGGCATCAGATCCCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAAAGTGGCCAGTGCTCTTAACTGCTGAGCCATCTCTCCAACCCGAAGGTTTTGGTTTTATTTTGTTTTGATTTGTTTTGTTTTGTTTTTGAGATCCTTATGAGAACCATCAGAATTATTTTATCTCCTACATTTATAGGTATGTGTGTGTGTGTTTGAATGTGCGTAGAAGCACAAGTGTGTGCAGGGACAACATACCTGTGTCCCAGTTCTTGTAGAATTCCACACTGGGCCTCCTCCCCCATCACCTTATTCATCGAGGCAGGATCTCTCAGCTGAACCCAAAGCTCTTGGGTTCCTGCCCCCATCCCTGCCTTCTAGACAGGTCACCATACCCATGGACCTGGAGGCTTGAACCCCTGTGCTAATGCTTCTGCTTTAACCACTGAGCCATCTCCACAGAATGGTGGCAGTCCCCTTTCCACAGTCACTATGTGCCCCAGCCTGCCTTGAGCCCCAGACAATCCTCTGCTCTGTCAGCCTTCCTAGTTCCAGAACTCTGGGTATGTAATACAACACCTTGATGTTACTGTTTTTATTTTTAATCAGGTCTGAGGCATTGTAGCTTTTGCTTACGGCCAACAGAGGTCCTCATGGAGTTCCGTTATTGTCTAACCCTCCTCTTCATAATCCCATTACCATCCTGGAATATGTAGATGCTAAATCCTGGGTTATCCACCTGTTCCCCACTCTCTCCTGCCACCCCTGTTGAATGTCCCATCCTTTCTCCTCATCCTCTCAAGGATACCCTTACCTGTGTATTCCCACCAGCATCCACTAATAAGCTGGTTGTAGCCAGTGTGGCCGAGTTGGGAAAGCAAGAGAAGAGAGAAGAAATGAGGTTGGAGACACCGTGGGCCAGGAGTTCCTAGAAGGACACAGCAGGATGAAGATGGGGAAGACGTAGGCACGGGGTGGGGTGGGGTGGGGAGGAGTGAGGCTAAGGGGCTTAACTCTGAAAAGGGATGACATTCACAATTAAGTCACACCTGATTGGGCTCAATAGTGTAGCTGTACTTGTCTGCATAGATGGAGGCCAGAGAGGTGGACACAGCAAAAGTCACCAACGAGATAGGCAGGGAGTCGGCCAGTATCCTGGGCAGCTCATCCAGGGTGGGGAGGAGGGGTTGGGGAAATCTAGGGAAGGAGAAAGCCAGGATAAGGGTGGGTCCGCCGCCGGTGCTACAGGGAACAGAGGAGACAGGAAACAGGAAGGGGCGGGGCAGGGGAGGGGTATCTTGGGAGAGGTGGGCGAAGTCTTTCTCTTACCCTCCAGGCAATGGTCCCACTACCTGGACATTGTATCTTGTGTCCAGGGAAGAGGTGAAGCAGAGCACCGTAGCCAGAAGCACCTGAAAACGATCACACCTTTTGTTTCGTTTTGTTTGTTTTTGTGTGTGTGTTTGTGTTGGGTTTTTGTTTGTTTGTTTGTTTTGTTTTGTTTTTGAGACAGGCTGTCCTGGAACTCACTTTGTAGACTGGCCTCGAACTCAGAAATCCGCCTGCCTCACAAGTGCGTGTGCCACCACTGCCCGGTTTAGAAGAAAGAGGTTTAACATGAAAGAGAATCCCTTTGCGAGTGTGGGGGCCTCTCTAGAGTGTAGCAGCTGATGAGGCCACAGACTAACTCACCTACACTGAAGGAAGAGTCCGTGTTTGCCCGGAGAGAGGGACAAAGGATCTAAGAGGAGGTCCTTTGGGATTGATGGACACAAAAGACACACTTGAGCCAAGTGAGCCATTCTAAGCGGAGAGCACATGTGAGTTGCCTGTGGAAAGCTGTGGCATTCATTTACACTGGAGGAGTTATCTGGGCGGGCAGTGAAAGGAATTACAGTGAGGGTCCCCGAGGAGAGAAAAAGTGCAGTGGGAAGGAAGGAGAGGAGCAAAGAGAGGCCTGACGCTCGACTCCGGTAGTGGAACAGTTCAGGTGTGTGTATGTGTAGCGGGGGAGTTCGGATGGCTCAACTGCTCTGGAAAGATGCTTGGAAAAGTACGCGTCACCGTGATCTCTCGCTTGTGACTCCTGAGATTTCCACAAGGGGGCGACAGCCTCTAGATCTCACTGGATGGGGAGGATCCGGATACAGCGGGGTCCAGGGATCTAGAGCCGCCCTCACCATGACAACTTCTCCTGGGATTGGAGTAAGTAGCCTGTCCCGGAATCGCACGTTTAGTTCCTTGACTGGCACTAGCAACACCAGGCTGAGCGCCGAGATGGTCACTTCTGCAGGACTGCTCTGGGACAGGGCGCTGAGCACCGCGGCCAGCGTCTGCGGGCAGGGACAGTGTGCAAAGGCCGGGTACCAGCGTTAACTGACCCCGAGAGACTCAATACCAACCCACTAGGCCGGGGCAAAACTCTCTTTCCAGAGGCCATCAAGGCTTCTACGTCCCCAGCGCTCTCACGCAGCGCCATAGTTCCTTGCATTCATTCCTTGTTGCCTATCTTAACCCCTTCACCCACCGTCCCTATCGAGCCACCCTTTCCCGTGACATCTAGAAACCTCTTTCCCCACCCCCACCTTGAAGAGAGAAAAGCAGCCGATCTGGCGTGGGAGAGACAATCCCAAGAGGCTTGGCAGCTGGGACACCAGCACGTGCAGCGCGGCCCCGCTGGTCAGCGCCTTGATCACAGGCTCCGATAGAAAGGTGGACAGGACACCAAGCTGCAGCACGAACATCCCCAGCTGTAGAGAAGGCGGCGACAGGTGGGGTCGGGGCGTGGTGCGAAACCTCCAACAGACCAGCCCGCACAGTGCACCAGCTTCCCCACGATATATCTGCCACTCCGGACTGGAAGCCAAGTTCAATGGTGTTTTTATCCTCCCCTCTATCCTCTGGCACAATTGTGTTGGTCCTTTTCGGTGGATTCATCTAAGGTCCTCACCCGGGTCGAGTCCTCGCTTACCATTAGCGCCCCACTCCCGAAGGCCACAGCGGCTGCCGCCCCAACTCTCCGAGCTTCCAGTTGCTCCCTTTCGATTCCACTGAGGTTCCCCGCGAGGGGTTCGGGCACCACCCTCTCGACTACAGAACCGGTCATTAGACTGAGTACTGCGAAAGTTCCTGCAGTCATGGGCCAAATGCAGAGATTAGCATTCGTGCAGCCTGGGGCAGAGCAGCAGCCGTGACTCGCACAGTTCTGGAGTCTCACATGCGTCCCATCCGCTCTCTTAGAAGACCTTAGGATCACGAGCTGGTGCAGCAGCCAGGAACCGATGCGGAAGAGCGCACCCATGGAACCAGTGGGCAAGGAGAAAAGAGTGTACCCACTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTGCCCCTTCCTCACGCTCTCACTGGACCGTGAGCCACTGTGCACCTCTTTCCCTTCAAGGTCATGGTCCCTTCAAGTTGTTTCTTTTAACCAAATGTCACAGGGGACAAGGAGATCCTTGTAAACTGTCGCCTCTATACCCACTCCAACAAGCCTAGGGAGGCGACAGAGTTCTGTGCAAAGGGGGTGGAAATGGGTTGTGGATCCCAAGAGAGGTGGCAAGTAGGAGAGGGAAGGGGGCAGAGGGGAATACAGCCCCAAAGCTTCCAAGTAGCAGCCTTGCTAAGGTGTGTTTGACCCCTGCTTTTAATCCCAGCACGCAAGTGGCAGAGGCAGGATCTCTGAGTTTAAGACCACCTGGTCTGTGGGGAGCTTCAGACCAGCTAAGACTATAAAATGAGACCCTATCTCAAGGGATGAGGGTATGCCTCAAGTCCCTGTCTATTTTCATCCCAAAGAGAAGCAAAGTCATGAGCAGTGTGGCCACTCACCAGTGGACAGGTGTCTCCCAGTGCCCAGTAAACTGTAGATGAGAACAGGAAAGAAAGACGTGTAGAGTCCGAACACCGGGGGCACCGAGGTCAGGAGGGCAAAAGCCATGCCTGGTAGAAAAGTAGAAGGAATCGGGGTGGGGTGGGGTGGGGATGGGATGAGGGTGGGGATGGTGGGGTGCACAACCTCCAGATACTGCCCTTCCCAACACTCTTGGGTTGACACCAGATACTATCAATCATGGCCCCGGAGGGAACGGAAAGGTTAATTAATGGGCCTCAGTGGGAGGTCGTGAGGCTGACAAGGTCACAGATCCTTGCCCACGTCCTGGGTGGCAGGTTTTGTGCCTCTCTACAATTCTCTGAGGCAGAACAGAGTCCAGTATATCTCGCAGAATTGCACAGATGTGGAGGGATGGGGGTGGGGAATTATGATGGAGTCCCTTAGTCCTCTCAAACCGAACCTCAAGCTCCTCCTCTCTGATCCTCCCCCATGCAGGGACACAAGTCCTCCACATCACTGCTGGACTCTCAGCTTCCCAGGGCCCTGCCCTGAAAGCCCCCCATAACTTGGAATTCCAACGGGCTGAAGTTGGGGACTCTTACCCTGGGGCACGTGCACAACGCCCACGGTTACTCCAGCCACAGCATCTCCGAGCAGCCAGGCTCGCCAGCGGTATTGGGGCAGCCAACGCAGCGGGGGCAGCCGTGCCAGCAGCACGCGCCACGCCCCCGGCCCAGAACAGGCGCTGGCTCGCCTCCTCCGCAGCCTGCACAAGCAGGGTAGGCAGGGCTCCGCTGGCAGCTCCGGCTCCGGCTCTGCGCCCCCGAAGAGCTCCTGGAACCGAGCGTGGGTGAGAGGTTCTCTGAACCTTGAGCTCAGCGGAGACTTAAGGTCAGAAACCTCTTCCGGATCTGAGCAGGTCCCAGAGGCAAGTGGCCCACTCATTTTGTCCTTGGCCCCCTGCAGCATCTCCTGGTCTTAAATACCCTCTGTTCCATCAGCTCTGAGCCCGCCTCTATAAAGCAGGGTCCAATCCGGACCTGTAGGGAGTACTCTCTAGAGTACCTCCTTGGGTCAGGCTTGGGTGGAAAAAGTCCCTGTGTTAGGCGTTTCCTCAGATATCCTTTCTTAGCTCTGCCTAAGAACCTGGGGGCCTGCTGTGAGGATGTTGAGGAACCATAGGGAGATAAGAGACCCACAGTCACTTCCAGTGACCCTCATCGCTTCGCTTCTCAGGGACTGGGAGGCATTTTTTTTTTCTTCATTTGCACTTTGAGCAAAGACAGAGCTGGTTCCTTTTGGGTCACAGGTCTTCTTTCCCTGAGCCTCTGCATTCTCACCACCACCACCACCACCACCACTGCTGGAGCACAAAATTCACAGCCAGTTGGGATTCCCCACTCAAATCCCATTCCTCATGTGGCAAACTTGCCCCAGTAAAGTACAAGAGGCCTTACTGTTTTATAAGCATGGTGAATGCGACAGGGTGCATGGTGAATGGGACAGGGACAGGGACCCACTCCACCCTCTCTAATGCAGATGGCTGGGTTTGATTTAGGGGAAAGACTGAATTAACAAAGACATTTTTATCTATTTGTTAGGAGCCCCAAAGTCCTCTAATACTGTTCCTTACATCCAGTGTTATTCTCCAAGTAGGCATTTAGAAGTACACGATTAAACTAAGGGCATGCCGTGTGCTGCTGGAACCGCTGCCCGTTGCTGCCTTGGGCACCTTGTCACCACTGGCAGTGGTGGGCCTGGCTGCCGCAGGAGCATGAAGGGTCCAGCCACCCACCTCATGTACCCCTGAAGAGACACAAAGGTTCTGGGCCCCAGGATTTCACTGTTTGCCGTCATCCACTCAGAGATGCTTCGGCACCCCCATCACCCTGTGGTTCCCCCAGTCCCTGACCCCAAAGCCCAGGGTGGGGTCCAGAGAGGGTGGGCCTCTCTTCTTCAATTCCTCCTATTGTTCCCCAGAAATCTTATCTGGGCTTCACGTCCTGTCCCCCCCTTAAAGATCCCTCCCCTCATTGTCAGCCACAGAGACCTGAGGCTCCCATATCTCCCCCCCCCCACCCCCCACCCCAGTAGTCTATTGTCTGCTGAGGAAGCCCCAGTGCACGTGACTGAGCCCAGGGGACTGACGGACACGGTGGACACACAGATACATAGGGACCCAGAGGCAGTCATATGGGTCTCCACACACTTGCCTGTCTCTGTTCCAGAGGCTGTGCCAGAAGCTGGGAGGGGCAGACATCTTCAGCTCTCCACATTAGCTCCGAATTTAAAGAATCTATTCCTACTCAGCGTGCACACACCTTACCCCTTTCCCTCAAGATGGCAAAGAGGACTCCAGAGCTGCTCCATCCTCCCATTCTTCAGCAGAGCATGGATGCTCAGAGGAAAGGTACCCGGGGTGTTTTGTGCTTTTAGATCTAGATACTGTGAGTGGTAGGGGGTCAGAGAACAGTGAGCCTTCTGGTGGAGTCTGAACCAAAGGATAGATCTAGAACCCAGGGCCCTGAATAAAGGGGAGAATACATTAAGAGATATTAAGATTTTCCCTAAGCCTGAGGTCTGGAGTGAGAGATGCTACAATTGATTAGAGGTGAAGGGCAAAGATAGAGGTCTCTTATCTTCTAACATTCTCATTCTATTGTTTTTGAGGGAGGGTCTCACTCAGTAGACCAGTCTGGCCTCCAACTCAGAGATCCGCCTGCCCCTGCCTCCCAGGTGCTGGGATTAAAGGTGTGCGCCACACATCCAGCTTTACCCTCTTCTTATCACCAGGGCCCTCCTCAAATGACTGCAGCCAGCAGACTTCTTCTATGCCACTTTAACAACCCATTCCCACGGCAGCCTCGCCATCTCCCTCCCAAGACAACAACCTCAGTGATATAGCAAAACTTTTATTCACAAGAAAACTCGAAAACAAAATCACAACCCAGGCCAATAATTTCTATCTGGTCCCTGCCCTCATCCTCCCTCCAAGCTCCTCCATATCCTCCATTTGAGTGGGTCGTGGTGCATGCAGGGAGTGGAACTGACGGATCCTGTGTCCCTTGGGCAGAAAAGAGTCCTTTCAGGCCCAGTCTCTCCCATCCTCTATACATCATGGAGGATGGATTCTGGCACATTTCCAGGATTCCCCCTCCAAATTCATTGAGGAATCATGCCTGCGCCAATAGCACTGCACTTTTGGGGTTACAGCTCATCTTCATCCAGCTTGGCGAGCCTGGCCTGACAGGGAGGAGTGTTCACAGTGGGAAGGGCTGGAGAGTCATGAGGAGTCTGGGGGGTATCACTGAGAGCCTGAAGGAAGATTCAAGTCAGAGGGTCAGCCAGGGTTACCAGTCAGAGGGTCAGCCAGGGCTACCAGCCTTCCCTCATTCCTCTCTCCAGTTAAAGACCCTAAAGATTCCTCTTGCCCCAGAAGGAAAGAAAGGAAAGTAAGAATGAAAATTTGGAAAGTCACACAAAAGGATAGTGGTGGAATAAGGAAATGGGGTGGGGGTGGAGACAAACGCCCTCCACTTTCTGCCCTAGTAGGAAACTATTCCCCATACCTGTGTATCCAGGGGCAACAGCACTCTGGACACCTCCCCTCTGGGCAGAAGTAGCAGGGAAGGGAGAGAGCCATTGATGGGTGTGTGCATGCTGGCCTGGGCCAGGTCTCCAGGCCGCATTCTCCCAGGGCTCCCTACCCAAGGCCCTCCTGGTCGCCCCAGGCTATTGGTCTGGCTCTCACGTCTCTGGTATTCAATGGGTGACTGTAATGCTGGGCCAAGGGAAGGAACAGAGATGTATTACAGAAGAGACCGATCACTTTCCCTGCCTCACCTTCTTCCTCATTTGGCCAATGTAAGGTGCTTGGAACTTTAGAGGGTTTCGGGTGGGTGTAGGATGGAGAGAAGTTAGGCTCAAAGAAGCAAGGGTTGGAGACTGCGTGGCTGGGGAAGGGGTTCAGGAGGTCAGATAGCTTTGGGAGCCTCTGAAGGGAGAGGATGGGAGCTTCACCGTTGAGAAAGTCACGCTGGCTTTCCAGGATCTGGGCCACTTGCTTTATCCAGGCTTGACTGACAGCAGGGTCTGAAGCCTGCAGAACGTAGCGCTGGATCCCACCTTCTGGCCCTCTAGAGGTCAGCGCAAACCGGCAAGGGTTGCCTTGGAGATTCCCCTCCAGCCCTAGGCAGCTCACCTGGATTGGCAATCAGAGACAGCTCTCAGATTCTGCAAGCCCTTCCACCAGCCCCAAAAGCCTGAGCCTCGCGCCATCCGGTCCGCTCCCTCCATATGCCCCGCCCCATCGGGGCTGCTTTCTCCATACTGCCCCGCCCCACCTTCCCCCTCGGTCGGCTCCCTCTATATGCCCCGCCCCACCTTGATGCTGTTCTTGTACACGTAGCCAGGCTGCGCGCCACCTCGGCCTCCTCCTCCCAGGGCCTCACTGAAGATGATGATTTGCTCGAACAGGAAGACACGCCTCTCCCGACCCCGGGAAGAAAGCAGACCCCCAGCTTCAGGCTCTGTAACCAAGAATGTGTCCTGGCCCAAGAGCTTCCCCTGAGCAGTCAGTTTTCCCTGGAACCGAAGGACATGGCCTCTGAGAAGGCGCAACAAGAGTTTGAAAAGAGAATCATCAGCTTCTCTCCCCTCCCCCACGCCAACACGCCCAAGGAGGTAATTCCTCATAAGGATCCAGACTACTAAGACCTGAGCTCAAGCGCAGTTCACTGCTCTGCCCCGCCCCATTCCCGTACCTCAAATCCCCGAAGTCTCCCCAGGGACATCATATCATTGCATCGCTTAGGTACGAAACACATGACCTCCACAGCTTGCTGGGACACAGGTGAAGAGAGGCGTCATTGGACAGATTCGTTACTGGCAGGGAAGCTCCTTTTGAGAGAGCCTCTCCAAGAATCCCCACCCCGGGGGCCTCTCCCTGAAAGCAGACATCCCTGAACCACCCTGAGACTGTGGTACCCACAAGATGAAGAGGATGAGGCAGATACAGTGTCCTGGGGACCTCACCTCCAGCTCTTCAGTATCCTTCCCAGCTCTTCTGTAATACTTTAGAAAATCCTATAACATAAAGAAGAAAGCTCTGTAGAGCCCGTCCCCAGCCAGCTGTATCCCCAGGGAGAAGGGTGATCCTGTTCCCAGCTCTGCCCCCCAGAGACAAGGGTAGTTTTGCCCCCATGGAGAAGGGTGGTCCTGTCCCCAGCCATGTCCCCAGGGACTAAGGTGGTCCTGACCTTCAGCAGCAACTGGTATTTCATGATTCGCTGCACTGGTTTAATGAGGAGGTCATTGAGCTGCAGTCGGTGTCCCAGCTGCTGCCTCAGCTCCTGGGGACAGGGACAGGGACAGGGACAGGGCAGTCATGTAGCCTGAAAAGTCTATTCACGCTGATCCTGTTCTTCTTTCCCCCAAGGCACCTCAGCCACTGACCTCAAAGTAGCTGTCCCCAAACTCTGACAGCACATGCTCTGACTTGGGTTTATTCTGGCAGTACACCACATACATATGCAGTCGGCGCTCCTAGCAGGGATCACGTTGAAGAAAGTGGGGGTGGGTGGTGACAAAAGAGAGCCCATCTCCACAGAACCCAGACTCACCCCAAGCCCTTCTTCCCCTGTTAAGGCCCAGCCTCACATGTTTGATGAACAGCTGAGCCAACCAATCGGGATCTTTCAAACACTGCTGCAGCTCCTGCAGGAAGTAGCTGGAAGAGTTGTGAGAGGGAGAGAAGCACCTCACACACTCCTCAGAGCGCTCACCTCCAAACGTCCCAGACTTTCAGCTACACGCCCTAGTCGCTCTCCCTACAAAACCCCAGTGGCTCCTTGAATCACATCGGACCCATGGTTCCAATACGGAGTCTAGGATAGACTACCAGAGAGCGCTCTTCCCCTGCGCCTGGCTCAGGGCTCCATCACTCACTCTCGGTGCCACTCATAGATCTGCTGAATGTTCCCAAACACAATCCTGTCACGACCTCGGAGACTCTCAGGTACCCCCTGAGTAGCCATGGTAGCCATGTAACCCTGTGGGAAGGACGAGATGGGACTATGATTACGTAAGCTCAGCCTCAGGTCGGCCCTGCACAAAGTCCAGAAGTAGAGGGAATCTGTAAAGTGACCAGAGGGAGCGACCTATCTGAAAAATATCCCCAGGCAGAGGGAAAGGGTCAGTGGGCCACGGCTCGGGAGGAGAAGCGAGAACTACCTCTACGATCTGTCCCAAGTCGTCCACATATGTTCTTTCTGTTTCCACCAGCTCACCCAGGACAAACCTAGAGAGCACAGCATGCCTTCACTTTAGAGTGTGGCTACATCCTTAGTTTAGCCTGTTCTTCTTGCACTAAAAGAACATCACCACAGCCCTCTCAGAGGGCAGTAAAATGTGAGGGATCTTGGGGAAGTTCAACCGAAGTGCCCTGTGAACTTACATACTCCTTTCCAGAGCCATCTTCTTCTGCTCTTCCTCATTCTTGGGAGCTTGGGACAAAGTTTCCTCTTCAGCTGGCTGGAAGGTAGGAAGTGGACAAACAGAGGATTGAATACCTTTCACTAAGAACCTTCTCAGATCTATTCTTTTGATGGTTTTAAGACCTGTTTGTAGCCGGGTTGTGGTGGGGCAGGCCTTTAATCCCAGCACTTGGGAGGCAGAGAGCAGGTGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGAACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAACAAAATAAAAAATTAAAAAAAAAAGGACTTGTTTGTAGGGTCTACATAGGTACATCATCTACATAGATAGAGTCTATGTAGTTCTGGCTGTCCTGGAACTTGCTGACCTTGAACCTACAGACATCCTCCTGCCTCTGCCTCTGGAGTGCTGGGATTGAAATTTATTATTATGATTATGATGATTATTACCATTTTTACATTATGGGGGGCCATGTGTAGATACCCA
GF3 -1 -1 1 GAGCAACTTGGAGAGGATTCTGCAG
GF4 -1 -1 1 GAATTAGCCTGATGAAGTTCCTTG
EX52 -1 -1 1 GAACTGACCTTACTGACTACATTGCTGGAG
EX32 -1 -1 1 GCCAGTGACTGAGGTGGTCAACAGTAGATG
GR3 -1 -1 1 GACTGACGGACACGGTGGACACAC
GR4 -1 -1 1 GACGGACACGGTGGACACACAGATAC
Comments:
Created
Target: 0402Ensembl ENSMUSG00000019467 -- 126623935 -- 126624378 ENSMUSE00000150624 -- ENSMUSE00000150618
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 12 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: 10, start 126623935: end 126624378
Found 6 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:10:126629878:126630877_146 G3:10:126619435:126620434_158
ACCEPT (0) gap pair G5:10:126629878:126630877_146 G3:10:126619435:126620434_708
ACCEPT (0) gap pair G5:10:126629878:126630877_146 G3:10:126619435:126620434_152
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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