High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by am9
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 55718
Chr: X
Strand: -1
Status: Ordered
BACs: RP24-574G11 : RP24-347L5 : RP24-321O24 : RP24-170E15 :
Instance: Plate 78: Well C03: BACs RP24-321O24 RP24-347L5 RP24-170E15 RP24-574G11
Instance: Plate 198: Well G01: BACs RP24-321O24 RP24-347L5 RP24-170E15 RP24-574G11
Oligos on GRCm38:
Type Start End Length Seq
G5 135671035 135671084 50 AATAATAGGACCAGAGAGTTGGTATCGGAGCCAGAGTTAGAGTAAGAGAG
U5 135665089 135665138 50 AAGACATGCACCACTATCACTACCTGGTAAGCAAGTTACTCTTAAATGAT
U3 135664912 135664961 50 TAGATGTTTTACTCCAAAAAGTAAAGGCACATAAGCAAGGTATTCTTTTT
D5 135664189 135664238 50 TCTTTCTTCTTTCATCCTGTGGCATTCTTTTTTTTTTTCTTTAACACTTC
D3 135664066 135664115 50 TGGCAGTCTAGAAACCGCCAAGATTGAATAGAAATGATTTCTCAAATAAG
G3 135660628 135660677 50 TTCAAAAGTGGAACTCAAGGAAAGAACAATAAAATCACAGTATTGAGAAT
Oligos:
Type Start End Length Seq
D5_D3 132198655 132198727 73 AGAGAATAAGTCTCTCTCTCCCTCCCACTCTCTCCCTCCCACTCTCCTCCTCCCACCTCCTCCCCTCCCTCTC
G5 132205574 132205623 50 AATAATAGGACCAGAGAGTTGGTATCGGAGCCAGAGTTAGAGTAAGAGAG
U5 132199628 132199677 50 AAGACATGCACCACTATCACTACCTGGTAAGCAAGTTACTCTTAAATGAT
U3 132199451 132199500 50 TAGATGTTTTACTCCAAAAAGTAAAGGCACATAAGCAAGGTATTCTTTTT
D5 132198728 132198777 50 TCTTTCTTCTTTCATCCTGTGGCATTCTTTTTTTTTTTCTTTAACACTTC
D3 132198605 132198654 50 TGGCAGTCTAGAAACCGCCAAGATTGAATAGAAATGATTTCTCAAATAAG
G3 132195167 132195216 50 TTCAAAAGTGGAACTCAAGGAAAGAACAATAAAATCACAGTATTGAGAAT
G5_U5 132199628 132205623 5996 AAGACATGCACCACTATCACTACCTGGTAAGCAAGTTACTCTTAAATGATCACTTGAAATAGCGCTTTTTTTTTTTGGTGTTTCTGGAACACTTAAAACATAGTAGAAGTTGAGTAAAAATGTTTTATTGAGTGAATGTTTTAAAAGTAGTTACCTAAACCTCAAACTGTATTTCATACGAACAAATGGAAGTCAATACACCCCATATATGTTCATACAGTAAGCTACTTAGACCATTAATAATATGAATATTAATAATCCACAGTCTAAAGTGGTCCTGGGACTTTATAGCAACATAGATAAAGCTGGATACTCACCTGTTTTATGTTTTTTATAAGTTCAAGAACACAAGAAAAGTCAGGAGGGGTTTGTGACAGTTGATCTCTCAAGTGGTCCCAAAATGCATTGTACATTGTCTCCACGAATTGGCTTTCTGGACTATTAAGAAAGTTAAATATGAAAACTGACTTTTACCTAGAGGTTTGATACAGGATCATGATTCTTCAAACAGTAGTTAAAATCATGGAGAAACAAACAAACAGACAGATAGTCCAGAAAGGAGTTATGATTTGAGAAGTGCTGAAAATTTAACTGTAGTTAGTCTCAGATGAAAAACAACACCCTTGGATCCTCTCAAAATGATACCAGAATGCCAGTCATTCTCTGTCTCCCTGCCCCTCAACCAAATGCCCTACATTCTCCCTCTGACCTTCCTTCCCTTCCTCCCAATATCTCCCTGTGTTTGTTTGTCTCTTTCTCTCTCCCCCTCTCAATACAAATCTCTGTCTCTGTCTCTGTCTCTCTGTCTCTCTCTCCCTCAATATTTTTCTTAGGTTGTATCTGTCTCTGTTCTCTATGTGTTCTCTTTCTCCCTCTCCCTCCTCCTCCATCTCCCTCTCCCCCTCCTTCATCTCTCCCTCCTCTCCTCCTCCCTGTTCTTCTCTCCTTTCCCTCCCTTTCCCTCTCCCTCTGTAAGAAGCATAAACTAATATTGGGACTCTGAATTTTGACCTTAATACTGCATCTAGCCTAGATGTGTGGCTTCATTTATATTTCCATATACATAGTATGTGATATTTTTCACTTAGGTATGAAATGTAGGGAAGGATTTTTTCATGTTTATAATGCTGAAGGATTTCATACACTTCACAGAAGGACTAAATAAAATTTACTACTTCCCCCTTTCTAACCTGCTCTTCACTTACCTTACTCCTAAAACCAGGAGGTTTACTATTTCTCAAAACACTTTTGCAATAACAGTGAACAATTTGTTTATAAATACAAATGACACCAAAATGTCAAGTTTCTCCCCAATATATCAATCTAACTTGTACGTGGAGATCAACTATCACTCATCTGATATGTTCCAGAGTTTTGAACTCCACAGTTACTACCCCTTACTATCCATCTGGTCAACAAATTCCAGAGTCTGTTTCCTAAGTGTCTCTGGAACCCTTCTTAGCCAAACATCCTGTACATTTACAATAGTCTATAAAGTGACCATCTTGCTCCAATTTGTAACATGTCCTTGCTAAAATTCAATGATTTCCCAAAAAATCCAAATCTGTTTGTTCATCCTGTACTGTTCATCTAATATCTTTCAATAACTTGATAACAGAAGACAGATGCATAGCCTCAATTACTCAGAATGATGCTATTCTGTCAAAATTCCCTAACTTTGAATCCTTCCTTCTTGCAACACCTTGCTCTGAAGCAATAATAATCTCTTTGGAGTGAGTAGTAATTACGTTCTACCTTTCTATGTCCTTGGAGAGACATTTCCTCTTAAGAGAGTACTTTTCACAATAGATCACCTAAAATATACTGAGTCATACTCTTGAGATTTAACTCAAGTGGTATGACTTCAAGACAGTCTTCCCTGATAGTCTCCCATGAAACTCCATATCTCATGAAGAACTCAATTCCCTAGCTATGGGAGAATTTATCCCTCTGCACAATAGTACATAGATTATTCTACAAGAACCTCTCAGTGGCAAAGAGAACATCTTACCTCTAATGTTCTCAGCACCCAACATTCTACATTGCATCTGATAAGTACTCAATAAATGTGTGTTAAACAAAAGAATGACAATGGTGTTTCCATTAAGTACTGTTAACGCTAATTAGTATCATTCCTAATCCCCAAAGTAAATTTAGAGAAAATAAATATAACTCAGATTCTAGGGACTATGAGCTGAACTAAGACAATCCTTCGTGTTTTGGTGAAAACTCTATTCATAAAAATGTACTTTGCATGTGTCATTATTAGCAACAAACAAAATAAATGCAACATACACAAGAATCACAAGAACCAAGATGGTACTTTAATAACAGAATCCCTAGAGTGTCAAAACAAATGAGGCATTCAAGGTGGGAAATTTAAAAAGCTATGAAATAACATTCTGGCAAAAAAGTATTCTTTTAACTCTTCATGCTTACTTACATTGTGTGTGTACATGTGCACACATGCAAAGTGTCACAGAATCTGTTTGGTGGTAAGAGGATAGATTATATAAGTTGGTTCTCTCCTTGCACCACGTGAGTCTCTAGGATCAAATAGGTCAACTGCAAGCACCTTTACCTTCTAAGCCATTTTCTAGCTCACATTTAAATGCTTTGGAAAGACTCCTTTATGTACCTCAATATCAATATAATGTAATTGAATTGAATCAATATAATTATGAAGAACATCAACAAGGCTTGTTTTTCTTAGTAAGTTAGAAAATATAAATCTGAAACTTCTTACCCATGACAAATGGCAATGTCATTATTAGAGAAGTTATTTATGGAGCTAGAGAGATGTTTCAGTGGTTAAGAGCCCTGGCAGCTCATCTCCAGGGCCAAGGCTTGAATCCCACAACCACATGGCAGCACAAAACTGTCTATAAATCTTGTCCTGGGGTATGCAGTGCCCTTTTCTGGTCTTCTTGGGCACTGCATGCATGTGGTACACAGACATATATGGAAACAATATACCCACATAGAGAAAACAAAATCAAAATTGAAAAAAAAGAAAAGTTGTCTATCTGCACCAACTTAATTTACAGAAAATTAGGGCTACCTTCTAGTAACTCTACCCTTCAAGTTTATCTGCAAAGATCTGAAATTCTTCACTAAAACGCAATACTTAACATTTTGACAATAATTACATTGGTTTATTTGGTATTTATATATAATCTGATATTGTCCCACCTGTGTGCCTGTATGTATGTGACTGTTGCCTCTGTATATGTAATCTATGTGCTCCTCTGGGAGTTTACATGTAGAGGCTGGAGGATGTCATATATCTTATTCTGTCACTCTCCACTTTATTCCCTTGAGACAGGACCTTTAACTGAATCAGGAGGTAGGGTGGCAGCCAGAAAGGCCCAGTTGTTGACTGCTTATTACTTTACTTAATTATTACTTTGTGTGAGCCTTTTGCTTACTACACTTAATGACTATATTGTGTGATCTCCTTGTTTACTACTTTGCTTGACTACTTTGTCTTTGATCTAAGAACTGGCCATGTTATTTTGGATGTACCTAGAATGATATAAAAGCAGACTAGAAAAGAATAAAGCTGATTCAGTCTCAGCACTGGCTGGAGTCATGTTATAGTTTTGTCTAATTGTTTGTTGTTGTTTTTTCTTTTCTTTTCAATCCTTACTCCCTTCCTTGAGATCCAGTTGAGTGACTGAGCTGGCTTGGTCACCCAGTAATCCTTTGTCTCTGCTGCACATATAGCTTGGGTACAGGGCATAAGTAGACAACACCATGCCATATTTTACATGGGTTCTGGGGATTTGAATTTAGGTCCTCATGTTTATAAAGTAACAGGGCTTAGCCACTAGATCATGCTTTATCCCCACTTGACCACTTGTAATTTTTATTTTTGTTTGCATCAGTGTTTTGCCTGCATGGAGATTTGTATGAGAGTGTCAGATACACTGGAAGTGAAGTTGCAATATGAGCAGTCATTTGGGCTCTGGGAACTGAATCCAAGTCCTCTGGAAGAACAGCCAGTGCTCTTAACTGCTGAGCCTTCTACTAGTGATTTGAACACTTTTACATTAAATAATTATAATGCTTACTCTGCTGTTCTATTTAATACTATTTAAGACAGACTCAATCCCTTCATGTTTTAATAAAACAAATCAAACATGACCAAAAATCATCTATGCTATGTAATTATACCAAGAAGAGCATTCATTCACCTAGTAATCAATCTATAAATAAAATATATACCTGTCTTCAGGTAAATCATTCTTTTCTATGGCAATTTCAGAAGCAACATTCAATTTGGAAACTTCATTAACTTTTCCTGTATCTTCATTAGAAATTGCTGCAAGCTGGTTCTCTAAAAAAAAGGTATTAGTGTATTAGTTATCAAAAGAAATCAGAGAGCTAAAGACAGAAACAGAGTGCCAGAGACATACACATAAATAGAGTCATAAAGCCATAGAGCCTGAGTGAGAGCCAGAAGTAAAGATACAGCTGAGAGACAGAACACCAGAGCCACAACCAGAGAACCAGAGCCAAAGATTGACAGTCATAGCCAGAGTAGAGAGCTATAACCAGAGAGCCAAGAGCCAAAGCCAGAACCAGGCACAGAGCCACAGATAGAGCTAGAGAGCCAGAAATCCAGAGCAAAAACTAGAGAGAGCCAGAGCCAGAGAGCCAGCAACATAGATCTAAAGAGCTAGATGAAGAACCTAAAAGCCAGAGCCATAGGCAGAGCGAGGACCAGAGCCAGGACCAGAACCAGATCTAGAGACCAGACCCATATCCAGAGACAGTACCATAAAAAAAAATCCATAGAAACAGAAAGCCAGAGAGTCAGAGTCATAGCCAGGAGAGAGCCAGGGAGTCAGAGTCATAGCCAGACCCAAATGCAGAGAGTCATACTTGAAGCTACAGTCAGATTCAAAGCCAGAGAGTCAGAGAGCCTGAGAGCCAGAGGCCAGAGGCCAGAGGCCAGAACCATAGCATGAGAGTGACAGTGAAAGCCAGAGCCAGAAAGCTGGCAAGCGAGAGAACTATAGTGAGAGACAGAGACCCAGAGATCTAATTCCAGAAAAAGAACCATAGACAGAGCCAGAAAGCAGGGAAGGCAGCAATCCAGAGACAGACAAAGCCAGAACCAGAGTCATAGGCAGAGCCTAAGCCAGAAGCCAGAGACCCAGAGGAGAGCCAGAGCCAGAAAGTCTGAACCAGAAAGCCTGAGGCAGAGACAGAGAGCCAGTGTCAGAAAGTCATGATCAAAACCAGATAGAGAGGAGATTCAAAGCAAAAGCAAAAGCTGGGCGTGGTGGTGCATGCCTCTAATCCCAGCACTTGGGAGGCAGAGGTAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGCCAGCTAGAGCCAGAGCCAGAGCCAGAGCCAATGCCAAAAACGAATTTCAGAGGTAGAGGCAGAGGCAGCCAGCCAGTGTCAAGGAGCCGGTGCCATAACCAGAGAGAGGCAGAGAGCCAAAGAGCCAGAAAGGCAGAAGCAAAGGCAGATATCCAGAGAGCCATGAGCACAGCCAAAGCTGGAACCAGAGAGTCAGTGAGGCAGACCAAACAAGCTGGAGCCAGAGCCAATGCCAAAAGCAGGAAGCCAGTCAGAAAGACACATAGCCAGATTCACAGAGCCAGAGGCAGAACCAGAACCAGAGCCATATGGATAGACCCAAATATATAGATATAAATATAGAGATAGTGATATAGGGATATCACCATAGTCAAAGCCAGAGAACAAGGGCTAGAGAGCCAGAGAGCCAGAGAGGCAGAGATCTGGAGGTCCAGAAAGCGAAAGAATAATAGGACCAGAGAGTTGGTATCGGAGCCAGAGTTAGAGTAAGAGAG
U3_D5 132198728 132199500 773 TCTTTCTTCTTTCATCCTGTGGCATTCTTTTTTTTTTTCTTTAACACTTCAAGATTCCACCAGACACAGCACTTTCATTGGATGCAACCCCTCAGCCTTGGACTTCTATTTACTTTTCTCTTTGTAATTTATTCATAATGAGATATGTTATTAACAACAGAAAGTACACTAAAAGAGCATGACTCTATGCTTGCCTCAGTAACTGTACTGGATCTTTTATGTTTTCTAATTCCTTTACAGCTTCATCTCTAATTGGTGCGCACATGAGGGCCATCAAGTTGAGAATATAGTGGGAGAGATGCAGAATATCCAGAGCCCCGTGCTCTGTTTCTTGTTTGAGGAGATCCAAATCCAGTACCTCTTCAATCTCATTTCTTAGAAGATTCTGACGTGGTAATAGTAGTGCCATTAAAATCTGCCCACAAAGGAAAAAGAATCAAAGGAGCCACATTATGTTTAGAATCCTAAACATGCACCCAGTGAACAGTACTTTCAAGCTAAAGGCATGTCAAACTTCATTTTCTAGGGTGTCAAAAAAAATGAGTCTCTAAATTTCCCTCTGTTTCGAAGATCATTATAATTCTAGGTTTCATGGAAATTATAGTAAGTCATTATTTTCAAGGGAAATGACATTAAAATCATAAATAAAAATAAGACTGGGGCAACAGTTAACTATAGACTCCACAAAATAATGATGCCCTTAACAAAAGTAAACGGCAATGTTAGATGTTTTACTCCAAAAAGTAAAGGCACATAAGCAAGGTATTCTTTTT
D3_G3 132195167 132198654 3488 TTCAAAAGTGGAACTCAAGGAAAGAACAATAAAATCACAGTATTGAGAATAGGAAAGGAAGTGAAAAGTACTGATTTCATTTAAAAGTAGCTAGTATTTAAACACGTAATCAAAAAAAATTAGTTGCTTTGAGTAAGGGCCTAATAAGAGCTGTCATGAGGGCTTCAGTTTTACAAATAAGTAGATTATATTATAAAAGGGCGTGAGGCTCAGAATTTAATGTTGCTTTATTTGACCGGATAATGCTATTTATCATGTTATAATGTATCAAGAATACTCTCACCTCCAGAACTGTAAGAAATACATCTATGCTTTTTAGAAATTACAAACACTTGAGTATTTTCCCTGAGCACAACAAAATAAACTAAGGGAGAAGAGTACTATTGAAATTTTGGACTGATGATCCACCAAATACCTGAAAATGTAGAAAATGGGCAGAATCTAAACATTTAAAGAAACAGGCTATTAAAAAAAAAAAAGGTTTGTTACAGGGCCCCCAATGGAGGAGCTAGAGAAAGTACCCAAGGAGCTAAAGGGATCTGCAACCCTATAGGTGGAACAACATTATGAACTAACCAGTACCCCGGAGCTCTTGACTCTAGCTGCATATGTATCAGAAGATGGCCTAGTCGGCCATCACTGGAAAGTGAGGCCCATTGGACTTGCAAACTTTATATGCCACAGTACAGGGGAAACAGCAGGGCCAAGAATGGGGAGTGGGTGGGTAGGGGAGCAGGGTGGGGGGAAGGGTATAGGGAACTTTCAGGATAGCATTTGAAATGTAAATGAAGTAAATACCTAATAAAAAATTGGAAAAAAAATTTAAAAATCAGACATGAAAAAAAAAAGGTTGTTAATGTGACTGTGGTGTTAAGGATGACTTGAATGAGGGCTAAGAAGGGAATAATATTTGTAATATGTTTGATGCTTTTGGACATTACTAAAATATTCATTATCAAAATTTTGGCAGAAATATAGATACTAAAGACCAGTATGATGAAGTTTCAAATTAAAATGAGAAGTCTCACTGGAAAACAGATAGCCTTACCTTATAGGAAAGTAGCTTAAAAGTAGCCCTTGATATGAAGTAGCAAATACTTTGAATTGTGTCCAAATTCAAAGTGTTTTACAGAATGTAAGTTTTGAGAACAATGTCCTAAGATATTAGGCAATAGAATTTTCTCAGCATGATACTGAAGGAGTTACCTGGCTAGTTTTAATCACATGTAGAAATACATGGAAAGAAAAAAAAAGAAAATTGAAACATAAGGAGAGTAGACTAACTTGAAAAATTAAAACTGACAATGTAAATAGTAAAAACCCAATAGGATCAAACCAAATATACCTAAGTAATTCCTTTTCTAATGAGATGAGTGTGGACAGAAGGAAATTATATGCTGGTCACACAACAATGGGAAATACACCTCCTAATGAATTTCAAAGATCTTAGAGGCAAGTAAGAACCTTTATTGTAAGATTATAGAGACATACCTACAGGATGTCACCTTTTGCTTGCCAAGAATACCTAGAGACTCTGCTCCCTGAATTTCAGTATAGAGCCCCTCAGCCAGCACAACTACCACAATGCAAACAAGCTCAGGTGTATATTAGGTTGGACTATGACTCCACTCAAAAAGTCTGGAAAACACAATGGTAAGCCTTAATAATCCCCTAGTAGCACTAATTATGTAAGCACACAGAATACATGAGCTACAAAGCAATAACAGTCTCCATCTTGGTTTCAAAAACATATGTGAAACGATCTGGCAGCCTAGGCAAAAGTCTGCCATAGAGGTAGAGTTCACAGAGTATCCACACACATGGCAATGTCTAGTGGAGCTGCTGGGCAGCCTCCCAAGAGTCCAGCATTATAGAACATCCCTGTACGGAATGCTACAAGTAAGAGACTCAAATTCATAATCATGCCTCATACAGGATGAGCCCAGTAAAACTATGTGTGTTAGCCTGACAAAAACTATTAAAATGCCTTTTTTTTTGGAAAAACTCTTTATGAAACTGAATAAGACATAAATAAATGCACCATTCAGGGACCAAAGGCCAAAGGGTGAGTTGGCAGCAGAGCTAAAAAGAGACACATAGTATGACATTTCAACAACCATTCTAGAGCTCTTTGCACTGTGCCTACCCTACTACATTTGGAAAATGGTATTGATAGAAGGAAAATTCACCACAGCACTAACAATTTTCTATGAAAGGTTAGAATGTGGGTGATTAATTTCCAATTCATATAATTTTTCTTTTCTATTTGTTTTAGCTGTGAAACACTGAAAGTCTTCATTTCTGTTTTTGCAGAGTTCAGACTGGGAGAAATAGAAGAACACATGAGCTGGGGTGGACGGAAATCTGTAGAATTATTACTATACTTAAATGCCTTAAGAGCATTTCATGAAATGTATGGAATCTCAGTGATCATTTACATGGCATCAAAATCAACCAGAGACACTGACTGCCCTTACCAAGTAAAAGGGCAGGGAATAAAAAGGTAGTGCATCTTAGCCTGGGACATAGAATATGTTTTACACACTGTGTAACAGCTAATGTGGAGACTCAGCTGGTCAAAGTGCTAAAATAAGTGATTAATGAGTGCCCAGACATAAAAAAGACACACATATCATCACTCCCATGGTTCAGCAGGCATTATAGAAGATAAGATGGAAAGATTGTTAGAGCCAAAGGATGAAGAGGAGTAATAGAAAATGCTATCTTCTGGATTTGGCAAGGCCATTACAATCACAAACATGTAGCAGTTACAGATACATGGACACATACTGCATAAAATCAGAAACAAACTTCAGAATAGGAGTAATTGGGAAGAAAAAATGGATCAGCAAGATTGAAAGAGGGATGATAAAGAATAATATGCATTGTTTATAGTATGAAATTCAAAAATATTAAACAAGAGTGGCTGGAGAGTTTGCTCAGTTATTAATGGCACTTGTTGTTCTTACAGAGGACTGGATTCATTCCCAGACGTCACATGGTGGGTTAGAAATATCTATAACTGCAATTCCAGAGATCCAACATTGCTTTCTGGCCACAGCAAGTACTAGGCATACACATTGTGCACATATATTTTTTTGCAGGCAAGATGTTCATAAACATAAAATAAAATAATTTAAAATAAATAATTTGTTAAAATTTAAAAAGTAAAAGTAGAAAATAGAAGTGAAAACTTATGTTAGCCATGTCTTCAGAATAAGACAGAAGTCTATTAAAATATTTGGCTCAAATTTTCTTTCATGGTACATAACAAGGTAGATTTCAGGAGCAGAGTATGGTATTGTTGGAAAATAGGTAACTGCTCAAAAACTAGTAAACTTCAATCCCAGCTGTGAGTTACTGAGAGAGTGGAAAGCTTAATCCAATTATCAAGTTTATAAATGAAGCCTTTAGACCATCACCAGGATTACACAAGAATGGCAGTCTAGAAACCGCCAAGATTGAATAGAAATGATTTCTCAAATAAG
U5_15 132199628 132214678 15051 AAGACATGCACCACTATCACTACCTGGTAAGCAAGTTACTCTTAAATGATCACTTGAAATAGCGCTTTTTTTTTTTGGTGTTTCTGGAACACTTAAAACATAGTAGAAGTTGAGTAAAAATGTTTTATTGAGTGAATGTTTTAAAAGTAGTTACCTAAACCTCAAACTGTATTTCATACGAACAAATGGAAGTCAATACACCCCATATATGTTCATACAGTAAGCTACTTAGACCATTAATAATATGAATATTAATAATCCACAGTCTAAAGTGGTCCTGGGACTTTATAGCAACATAGATAAAGCTGGATACTCACCTGTTTTATGTTTTTTATAAGTTCAAGAACACAAGAAAAGTCAGGAGGGGTTTGTGACAGTTGATCTCTCAAGTGGTCCCAAAATGCATTGTACATTGTCTCCACGAATTGGCTTTCTGGACTATTAAGAAAGTTAAATATGAAAACTGACTTTTACCTAGAGGTTTGATACAGGATCATGATTCTTCAAACAGTAGTTAAAATCATGGAGAAACAAACAAACAGACAGATAGTCCAGAAAGGAGTTATGATTTGAGAAGTGCTGAAAATTTAACTGTAGTTAGTCTCAGATGAAAAACAACACCCTTGGATCCTCTCAAAATGATACCAGAATGCCAGTCATTCTCTGTCTCCCTGCCCCTCAACCAAATGCCCTACATTCTCCCTCTGACCTTCCTTCCCTTCCTCCCAATATCTCCCTGTGTTTGTTTGTCTCTTTCTCTCTCCCCCTCTCAATACAAATCTCTGTCTCTGTCTCTGTCTCTCTGTCTCTCTCTCCCTCAATATTTTTCTTAGGTTGTATCTGTCTCTGTTCTCTATGTGTTCTCTTTCTCCCTCTCCCTCCTCCTCCATCTCCCTCTCCCCCTCCTTCATCTCTCCCTCCTCTCCTCCTCCCTGTTCTTCTCTCCTTTCCCTCCCTTTCCCTCTCCCTCTGTAAGAAGCATAAACTAATATTGGGACTCTGAATTTTGACCTTAATACTGCATCTAGCCTAGATGTGTGGCTTCATTTATATTTCCATATACATAGTATGTGATATTTTTCACTTAGGTATGAAATGTAGGGAAGGATTTTTTCATGTTTATAATGCTGAAGGATTTCATACACTTCACAGAAGGACTAAATAAAATTTACTACTTCCCCCTTTCTAACCTGCTCTTCACTTACCTTACTCCTAAAACCAGGAGGTTTACTATTTCTCAAAACACTTTTGCAATAACAGTGAACAATTTGTTTATAAATACAAATGACACCAAAATGTCAAGTTTCTCCCCAATATATCAATCTAACTTGTACGTGGAGATCAACTATCACTCATCTGATATGTTCCAGAGTTTTGAACTCCACAGTTACTACCCCTTACTATCCATCTGGTCAACAAATTCCAGAGTCTGTTTCCTAAGTGTCTCTGGAACCCTTCTTAGCCAAACATCCTGTACATTTACAATAGTCTATAAAGTGACCATCTTGCTCCAATTTGTAACATGTCCTTGCTAAAATTCAATGATTTCCCAAAAAATCCAAATCTGTTTGTTCATCCTGTACTGTTCATCTAATATCTTTCAATAACTTGATAACAGAAGACAGATGCATAGCCTCAATTACTCAGAATGATGCTATTCTGTCAAAATTCCCTAACTTTGAATCCTTCCTTCTTGCAACACCTTGCTCTGAAGCAATAATAATCTCTTTGGAGTGAGTAGTAATTACGTTCTACCTTTCTATGTCCTTGGAGAGACATTTCCTCTTAAGAGAGTACTTTTCACAATAGATCACCTAAAATATACTGAGTCATACTCTTGAGATTTAACTCAAGTGGTATGACTTCAAGACAGTCTTCCCTGATAGTCTCCCATGAAACTCCATATCTCATGAAGAACTCAATTCCCTAGCTATGGGAGAATTTATCCCTCTGCACAATAGTACATAGATTATTCTACAAGAACCTCTCAGTGGCAAAGAGAACATCTTACCTCTAATGTTCTCAGCACCCAACATTCTACATTGCATCTGATAAGTACTCAATAAATGTGTGTTAAACAAAAGAATGACAATGGTGTTTCCATTAAGTACTGTTAACGCTAATTAGTATCATTCCTAATCCCCAAAGTAAATTTAGAGAAAATAAATATAACTCAGATTCTAGGGACTATGAGCTGAACTAAGACAATCCTTCGTGTTTTGGTGAAAACTCTATTCATAAAAATGTACTTTGCATGTGTCATTATTAGCAACAAACAAAATAAATGCAACATACACAAGAATCACAAGAACCAAGATGGTACTTTAATAACAGAATCCCTAGAGTGTCAAAACAAATGAGGCATTCAAGGTGGGAAATTTAAAAAGCTATGAAATAACATTCTGGCAAAAAAGTATTCTTTTAACTCTTCATGCTTACTTACATTGTGTGTGTACATGTGCACACATGCAAAGTGTCACAGAATCTGTTTGGTGGTAAGAGGATAGATTATATAAGTTGGTTCTCTCCTTGCACCACGTGAGTCTCTAGGATCAAATAGGTCAACTGCAAGCACCTTTACCTTCTAAGCCATTTTCTAGCTCACATTTAAATGCTTTGGAAAGACTCCTTTATGTACCTCAATATCAATATAATGTAATTGAATTGAATCAATATAATTATGAAGAACATCAACAAGGCTTGTTTTTCTTAGTAAGTTAGAAAATATAAATCTGAAACTTCTTACCCATGACAAATGGCAATGTCATTATTAGAGAAGTTATTTATGGAGCTAGAGAGATGTTTCAGTGGTTAAGAGCCCTGGCAGCTCATCTCCAGGGCCAAGGCTTGAATCCCACAACCACATGGCAGCACAAAACTGTCTATAAATCTTGTCCTGGGGTATGCAGTGCCCTTTTCTGGTCTTCTTGGGCACTGCATGCATGTGGTACACAGACATATATGGAAACAATATACCCACATAGAGAAAACAAAATCAAAATTGAAAAAAAAGAAAAGTTGTCTATCTGCACCAACTTAATTTACAGAAAATTAGGGCTACCTTCTAGTAACTCTACCCTTCAAGTTTATCTGCAAAGATCTGAAATTCTTCACTAAAACGCAATACTTAACATTTTGACAATAATTACATTGGTTTATTTGGTATTTATATATAATCTGATATTGTCCCACCTGTGTGCCTGTATGTATGTGACTGTTGCCTCTGTATATGTAATCTATGTGCTCCTCTGGGAGTTTACATGTAGAGGCTGGAGGATGTCATATATCTTATTCTGTCACTCTCCACTTTATTCCCTTGAGACAGGACCTTTAACTGAATCAGGAGGTAGGGTGGCAGCCAGAAAGGCCCAGTTGTTGACTGCTTATTACTTTACTTAATTATTACTTTGTGTGAGCCTTTTGCTTACTACACTTAATGACTATATTGTGTGATCTCCTTGTTTACTACTTTGCTTGACTACTTTGTCTTTGATCTAAGAACTGGCCATGTTATTTTGGATGTACCTAGAATGATATAAAAGCAGACTAGAAAAGAATAAAGCTGATTCAGTCTCAGCACTGGCTGGAGTCATGTTATAGTTTTGTCTAATTGTTTGTTGTTGTTTTTTCTTTTCTTTTCAATCCTTACTCCCTTCCTTGAGATCCAGTTGAGTGACTGAGCTGGCTTGGTCACCCAGTAATCCTTTGTCTCTGCTGCACATATAGCTTGGGTACAGGGCATAAGTAGACAACACCATGCCATATTTTACATGGGTTCTGGGGATTTGAATTTAGGTCCTCATGTTTATAAAGTAACAGGGCTTAGCCACTAGATCATGCTTTATCCCCACTTGACCACTTGTAATTTTTATTTTTGTTTGCATCAGTGTTTTGCCTGCATGGAGATTTGTATGAGAGTGTCAGATACACTGGAAGTGAAGTTGCAATATGAGCAGTCATTTGGGCTCTGGGAACTGAATCCAAGTCCTCTGGAAGAACAGCCAGTGCTCTTAACTGCTGAGCCTTCTACTAGTGATTTGAACACTTTTACATTAAATAATTATAATGCTTACTCTGCTGTTCTATTTAATACTATTTAAGACAGACTCAATCCCTTCATGTTTTAATAAAACAAATCAAACATGACCAAAAATCATCTATGCTATGTAATTATACCAAGAAGAGCATTCATTCACCTAGTAATCAATCTATAAATAAAATATATACCTGTCTTCAGGTAAATCATTCTTTTCTATGGCAATTTCAGAAGCAACATTCAATTTGGAAACTTCATTAACTTTTCCTGTATCTTCATTAGAAATTGCTGCAAGCTGGTTCTCTAAAAAAAAGGTATTAGTGTATTAGTTATCAAAAGAAATCAGAGAGCTAAAGACAGAAACAGAGTGCCAGAGACATACACATAAATAGAGTCATAAAGCCATAGAGCCTGAGTGAGAGCCAGAAGTAAAGATACAGCTGAGAGACAGAACACCAGAGCCACAACCAGAGAACCAGAGCCAAAGATTGACAGTCATAGCCAGAGTAGAGAGCTATAACCAGAGAGCCAAGAGCCAAAGCCAGAACCAGGCACAGAGCCACAGATAGAGCTAGAGAGCCAGAAATCCAGAGCAAAAACTAGAGAGAGCCAGAGCCAGAGAGCCAGCAACATAGATCTAAAGAGCTAGATGAAGAACCTAAAAGCCAGAGCCATAGGCAGAGCGAGGACCAGAGCCAGGACCAGAACCAGATCTAGAGACCAGACCCATATCCAGAGACAGTACCATAAAAAAAAATCCATAGAAACAGAAAGCCAGAGAGTCAGAGTCATAGCCAGGAGAGAGCCAGGGAGTCAGAGTCATAGCCAGACCCAAATGCAGAGAGTCATACTTGAAGCTACAGTCAGATTCAAAGCCAGAGAGTCAGAGAGCCTGAGAGCCAGAGGCCAGAGGCCAGAGGCCAGAACCATAGCATGAGAGTGACAGTGAAAGCCAGAGCCAGAAAGCTGGCAAGCGAGAGAACTATAGTGAGAGACAGAGACCCAGAGATCTAATTCCAGAAAAAGAACCATAGACAGAGCCAGAAAGCAGGGAAGGCAGCAATCCAGAGACAGACAAAGCCAGAACCAGAGTCATAGGCAGAGCCTAAGCCAGAAGCCAGAGACCCAGAGGAGAGCCAGAGCCAGAAAGTCTGAACCAGAAAGCCTGAGGCAGAGACAGAGAGCCAGTGTCAGAAAGTCATGATCAAAACCAGATAGAGAGGAGATTCAAAGCAAAAGCAAAAGCTGGGCGTGGTGGTGCATGCCTCTAATCCCAGCACTTGGGAGGCAGAGGTAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGCCAGCTAGAGCCAGAGCCAGAGCCAGAGCCAATGCCAAAAACGAATTTCAGAGGTAGAGGCAGAGGCAGCCAGCCAGTGTCAAGGAGCCGGTGCCATAACCAGAGAGAGGCAGAGAGCCAAAGAGCCAGAAAGGCAGAAGCAAAGGCAGATATCCAGAGAGCCATGAGCACAGCCAAAGCTGGAACCAGAGAGTCAGTGAGGCAGACCAAACAAGCTGGAGCCAGAGCCAATGCCAAAAGCAGGAAGCCAGTCAGAAAGACACATAGCCAGATTCACAGAGCCAGAGGCAGAACCAGAACCAGAGCCATATGGATAGACCCAAATATATAGATATAAATATAGAGATAGTGATATAGGGATATCACCATAGTCAAAGCCAGAGAACAAGGGCTAGAGAGCCAGAGAGCCAGAGAGGCAGAGATCTGGAGGTCCAGAAAGCGAAAGAATAATAGGACCAGAGAGTTGGTATCGGAGCCAGAGTTAGAGTAAGAGAGCCAAAGACATAGGCAGAGCCAAAGAACTGGAGCCAGAGATAGAGACTAAAAGCCAAAGGAGAGCCAGAACAGAAGCCAAAGCTAGAGCCAGAGATCCTGAGCCAAAAAGCCATAACCAGAGCCAGAGCCAGTGAGTCAGAAAGCTATAGAGCCAGACAGCCAGAGCCAGGAACAGATACAGAGAGCCAGAGCCAGGAACAGATACAGAGAGCCAGAGCCAGAACCAGATACAGAGAGCCAGAGCCAGAGCAATAGCCAGAGACAGAGGGCCAGAGAGTCAGAACCAGTCAAAACCAATGGGACCCACAGTCAGAACCAAAACCAGAACCTGAACCTGAGAGAATGTGCCATGGGCAGAGTCAGAACCAGAACCAGAGACAGACAGTGACAGAAGCCAAGAGAAAGAGCTATAGCCAGGGCTAGAACCAGAACCAGAACCAGAGCCAGAGAGCCAAAACCAGAGAGCCTGCGCCAGAAAGCCAGAACCAGATTCAGAGTCAGAGCCAAAGATAGATAACCCAATATTCAGAGTAACCCAAAGGAGATAGCCAGATACAGAGAGACAGAGACAGAGACTGAGACAGAGCTAGAGCCAGACAGCCCTTGGTAGAGCTAGAGCTAGAGCCAGAGGCAGAGGCAGAAGCGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGAGCCAGAGGCACAGCCAGAGACATAGCATGAGGTCCAGTGTAAGAGTGAGAGCCAGATACTAGGCTGGGGCCTAGCAAACACATAAGTGGATGCTCACAGTCAGTTATTGGATGGATCGCAGGGCCCCCAATGGAGGAGCTAGAGAAAGTATCCAAGGAGCTAAAGAGATCTGCAACCCTGCAGGTGCAACAACATTATGAACTAACCAGTACCCCGGAGCTCTTGACTCTAGCTGCATATGTATCAAAAGATGGCCTAGTCGGCCATCACTGGAAAGAGAGGCCCATTGGACACGCAAACTTTATATGCCCCAGTACAGGGGAATGCCAGGGCCAAAAAGTGGGAATGGGTGGGTAGGGAAGTGGGGGGGAGGGCATGGGGGACTTTTGGGCTAGCATTGGAAATGTAATTGAGGAAAATACATTAAAAAAAAAAAAAGAGTGAGAGCCAGACAGTCAGAGAGCCAGAGCCAGAGAATCAGAGCCAGAACAATAACCACAGCCCTACCTGGAACCTCAGAGTGAGAACAAGAGCCAAAGGTAGACAGACAGATAGGTAGATCTAGAGCCAGAAAAATAGCCAGACCCAGAGAACCAGAAAGGCAGAGATCCAGACAGACTTGAAGCCAGAGCCTGAGTCCCAGAGAGCCAGAAATCCAGAACAAGAAGCAGAGGCACATAGAGCCAGTGATAAAGAGACAGAATGAGGGCCAGACAGCCACAGAAGAGCCAGAGCCAGAGTCAGAGCCAGAGAGACAGAGATAGAGAAACAAACAGACAGAAACAGAGCCTGATCTAAATATCCAGAGAACTAGAGCCACAGCCAGAGTCAGAGTCAGAACTAGAAGTAGAGGGCCAGAGGGCCAGAGCAAGAGATCCAAAGCCATAGCCAATTCAAAGACAGAGAGCTGGAGTTAGAGAGCCACAGGGCCAGAATCACAGAATCAAGGGCAGAGAAGGAGCAAGAGAGGCAGGACCAGAGGCAGAGACAGAGTCACAAACAGAGGGCCAGAGACAGAGTCTGAGTCAACAACAGACTTAGAGCCAAGACAGGAGACATAGCAATAGCCAGGACTAGAGTTAGTGCCAGAGAGCTATTACAAGACCCAGAGAGCCAGAACAAAAGCCAGAGTCAGATCCAGATAAAAGGATCCTGAGCTGGACAAAAGCCAGAACAAGAGAGCCAGTGAGCCAGTGAGCCAGAGCTATACAACCTTATAGCCAGAGAAAGAACCACAACCTGATTAAGATATTCAGAGAGCCACAGCCACAGTCAGAACCAGAGATCCAGAGATCCAGAGAGCCACACAGCCAGACACACAGTGCTAGAGGCAGAGGGAGTGACAGAGTAGAAAAGTCACTAGCAGAACTGAAACCACAGAGGGTCAGGGACAGAGCCAGTGTCAAGAGCCAGAGCCAAAGTAAAAGCTAGAGCCAGAGTCAGAAAGAGTCAGAGAAATAGAGTCAGAGTCACAGTGCCAGAGAGTTAGGGACAGATGCAGAGAGGCAAAGCAAGAGCCAGAGCCAGAGAGTCAGAAACACAGACAGAGTCAGAATTAAAGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACAGAGACAGACACAGAGACACAGAGACAGAGACACAGAGACAGAGAAGAGAGAGAAAGTCCTGAGAGCCAGAGACATTGCCAAGAGGGCCAGCATCAGAGAGACCAAAGTAGAGCCAGAATAGAGACAAAGAGTCAGTGAGCCAGATCCAAGAGCCAAAGTGGGAGCTAGAGCCAAAAGCAGAGATTTAGATGGATCTGGAGTCAGAGACAGGGCCTGAGACAGATACAGAGAGCCACAGTCATAGAGCAAGAGTCAGGACCAGAGACAGTCAGAGATCGAGAGCCAGAGCCCAAATCAAAGCTGTAGCTAGATCCAGAGAGCCTGAGCCAGAAAGCCAGAGGCAGAGGCAAAGGCAGAGCTAGAGAGCCAGAGGGAGAACCAGAGTGATCCCCAGAGCCAGAGTCAGAAACAGAGCCAGAGAACCAGAGGAAAGCCAGAGCCCAACTCAGAGAGACAGAGACAGAGACAAAGACAGAACCAGAGATTCAGAGGAAAGACTGAACCCAATTCAGAGCCAGAAATCCAGAGCCAGAGCCAGAGCCAGAGCCAGAAATCCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGATGCAAAGGTAGAGGCAGAGGCAGAGGCAGGCAGAGAGGCAGAGAAGCAGAAGCAGAGGGTCAGCATATATCCAGAGTCAGAACTAGAGCCTGGGAACCAGACAGCAAGACAGCCATAGAGACAGAGACAGAGACAGAGACAGAGACAGAGACAGACATCTAGAGAGACAGAGTCACAGCCAAAGCCAGAACCAGAGGTCCAGAAACTCAAAGCAAGAGACTAAATCCAGAGACAATGCCAAAGCCAGAGGGCCAGAATCAGAGAGCTACCGGGCCAGACTCACTGAACTGGAGGCAAGACAGAGAGGCAGAGACAAAGAGGCAGAGCCGATGAGGCTGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCAAGAGCAAGAGCAAGAGCAAGAGCAAGAGCAAGAGAGAAATAGGTGAGTCAAAGCCAGGGCCAGAGAAGAGACCCAGAGGTAGAACCAGAGCTAGAAAACCAAAAAGCCAAGGCCAGAGAAGCAGAACCACAACCAGAGCCAAAACCTGAGCAAAAGAGTCAGAGACCCAAAGCCAGAACCAGAAAAAAAAAAAGTGCCAGAGCAAGAGAGCCAGAGTAATAGAGACAGAGACAGAGACAGAGACAGAGACAAAACTAAAGCTAGAGCCAGAGAGCCAGTAAGCCAGAGATCCAGAGCCAGAACTAAATACAGGGATCCAGAGTCACAGAGTAATAGCTAAAGTCAGACAGACAGAAAACCAGAGCCAGAGCCAAAGCTAGAGCCAGAGACCCAGAGCCAGAACCAGAGACAGAGACCAAGATCTAGAGCTATCGACAGAATCAGAATAAGAGCAAGATCCAGAGCCCTGTAACCAGAGCTAGAGCCCAAGTGATAGAGGAAAGCCAGCACCCAAGCCTGAGCCACAGTCAGAACCAGAGATCCTGTGCCAGAAAGCCAGAACCAGAGCCAGTGTCACAGATCAAGCCAGAAAACAAGAGATTCAGCGTCATCGCTAGAAGAGAGCCAGATCCAGAGATACAGAGACATATTCAGAGCCAGAGAGCCCTAGATAAAAATAGAGCTAGAGCCAGAGTCATAGCCAGTGCAGTAGTGGCAAATCCAGAACAATAGAATGAGAGCCAGAGTGAGAGTCAGGGAACCAGAGTGACAGAAAGACAGAGACAGAGAAAGAGACAGAGCCACAGCCAGAGACAGAGAGACAGCCAGCCAGAACCATAGCCCTAGCAAGAGCCTGGGAGCTAGAGAGCCAGAGATAGAGAGCCAGAATACCAGAGCCAAAGACTGAAACATAGTCAGAGCCAAAAAATGAAAGAGCGAGAGAACCAAGAACCAGAGGCAGAGAGCCAGGCATAGCACAAGCGAGAGACTGAGAGCCAGAGCTAGAGCCAGAGGCAGAGAGGGAGATAGTGAAAATAGAGCCAGAACCATAAACAGACCCAGATAGCAAGAAAGGCAGAGATCCGGAGACAAAGAAAAAAACAAAATTTAGAGAGAGTCAGAGCCAGAGCCATAGGCCGAACCAGAACAAAAGCCAAAGCCAACGACCAAGCCAGAAGCAGAAATCATGGGCCAGAAAGACAGAGACAGAGACAAAGGAAGAGGCAGAGGGGCAGAGGGGCAGAGGGGCAGAGGGGCAGAGGGGCAGAGGGGCAGAGGGGCAGAGGGGCAGAGGGGCAAAGGGGCAAAGGGGCAGAGGGGCAGGGGGGCAGGGGGGCAGAGGGGCAGGGGGCAGGGGGAGGGGGCAGGGGGCAGAGGGGCAGAGAGGCAGAGCCAGTGCCAAAGAGCCAGAGCCAGATCCAGAGGCAAAAAGCCATAGGAGAGAAAAGTTGGAGCCACAGAGCATAGAAGAGCACCAGAGGTAGAACCAGAGTCAGAGATCCAGAGAGATCAAGCAAGAGACAGAAGCACAACCAGAGACAGAGAGACTGACACAGACAGGCAGAACTAGAGCAAGAGCCAGAGCCTACTCCAGAGTCAGAGAGCTAGAGAGTCAGAAACCCATAGCCAGAACCAATAACAAAGTTCCAGAAACAGAGAAAGAGAGCTGGAGTAATAGCCAGATCTAGAGAGACAGAGAAACAGAACCATAGTCACAGTCAGAATTATGGGGCTAGAGAAAGAGTAAATCCCAAGACCCAGAAACAATGCCAAGAGTGTCAGAGTCAGAGAGACCAAGTTAGAGCAGGACAGAACCAGAGAGTCAATGAGCCAGATCTAGGAACCACAGTGGGAGCTAGAGCCAGAGGCAGAGATAAAAGCTGGAGCCAGAACTAGAGCCAGAGCCAGGACCAGAGAAAGAGCCAGAGACAGAACTAGAGCCACATCTACAGATCCAAAGGAGAGACAGAGCCCAATTCAAAGACAGAGACAGAAATTCAGGGCCAGAAATACTGAGTCAGAAATGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGACACAGCGCAGATCCAGAGTCAGAGCTAGAGATTTATATTCATAGAATGAGGGAGCTAGAGCTATAAATCCAGAGAGCCAGTGGACCATAGCCATAGACAGAGTGCCAGAGACAGAGACAGAACTAAAGCCAGGGAACCAGAGAGCAATATAGCCATAGAGCCACAGCCAAAGTTGGAACCAGAGGACCTGAGAGAAAACACAAAAAAACTAGAGCCAGAGACAATGACAAAGGCAGACCAAAATCAGAGAGCCACTGAGCCAGACTCAAAAAGCTAGAGACAGAGCCAGAGCAAGAGAAGCAGAGACAGGAAGACAGAGACAGAGGTAGAGCTGAAGTCAGAGCCAGAGGGCCAGAGACCCAGAGTCAGAGCCACAGCCAGAGGCAGAAACCCAGAGCCAAGAAGGCAGAGACAAAAACAGAGATAAAGGTAGAGCTGAATCCAGAACCAAGGGGCCAGAGACCCACACCCAGAGCCAGAAACCCAGAACCAGAGCCCAAGAGGAAGAGCCAGAGCACCAGATGCAGAGAGCCAGAGCCAGACAGCTATAACCAGAACCAGAGTCAGAGCAAGAGAATCAGAGATCTAGAGTCATAGCTTGAAGTAGACAGCCAGATCCAGAAAGCAAGAACCAGAGCCAGAACCAAAGCCAGAGGCAGAGTGATGGGAGAGTCAGAGCCAGAGCCTGAGAGACAGAGACAGAGAAAAAAAGCCAGAGATAGAACCAGAGCCAGAAAGTCAGAGTGCCAAGGCCAGAGAGGCAGAAGCATAACCAAAGACAGGGATACAGACATAAAGAGGCAGGGCCACAAGCAGAGCAAAATCCTGAGCAAGAAAGCCAGAGCCAGAACCAGAATCTAAGTGTCAGAGCCAGATCAGGACAGCTAGAATTATAGCCAAAGTCAGAGAGCCAGAGTCACAAGCAAAGCCAAAAACAGAGAGACAGAGAGACAAGAACCAGAGAGCCTGAGAGCCAGAGCTAGAGGCAGAGAACCAGAGATCCAGTGCCAGAGATAGAAACATAGCCAGACACAGAACCAGTAAGACAGAGACCCAGAGACAAAACCAAAGCTATGACCAGAGCCAGAGCTATAGGCAAAGCTAGAACCAAAGATACAGAGGTGAGCCAGAACCAGATCCTGAGGCAGAAAGCCAAAGCCAAAAATCCAGAGACAGAATCTGGGCCAGAAATCAAGACAGAGGCAGAGACAGAGGTAGAGAACCAGACCAGGTCCAGAGCCAGAACAAGAGACAAAGCCACAGCCAGAGAGCCTGAGATTCTGAGAGCCAGAGACAAAAGCAGAGAGCCTGAGATCCGGAGACACAGAGCCATAGCCAGAAAGAGAAATCCAGAGACAGAGACAGAGAGCCAGAGTGCAAGAGCCTGAGAAATAGCCTGAGCCAGAACCAGAGGCAGAGGCAGTGAACCAGCACATGAACTAGAGTACAAGATCCAGAGGCAAACCAGAACTCATGCCAGAGTTCCAGAGACAGAAAGCCTGAGAAAGAAAGCCAAAATCAGAACCAGACTTAGATCCAGAGTGAAAAGACTAGAAAGTCAGAGTCATAGCCAGAAGGGGAGAGCCGGAGCCAGAGACATAGAGCCATAGCATGAATCAGAAAGCCCTAACTAAAACTAGAGATAGAGCCAGAGTCAGATTCAGAGAGGCAGAGGTACAGCCAGAGCCATAATGTGAGAACCAGAGTGTGAGTGGGAGCCATAGTCAGAGAGCCAGAAAGCCAGAAAGCAAGAGAGACAGAGAGAAAGTACTACAGAGCTACAGAGAAAGAAACAGCAATAGTCAGAGAGACAGAGGCATAGCCCTACAAGAAACAGAGAAATAGGATGTCAGAACCAGAAAACCAGGGATTTAGAGATGCAAAGAAAAAATTGAAGCCAGAGCCAGATATCAATAGGAGAACCAGAGCCAGAGCAAGAGCCAAAAAACAAAAACAATCAAACAAACAACAGAGCTAGAAAGCCAGAGGTAGAAGCACAGGCAGAGGCAGAGAGGCAGAGAGGCAGAGAGGCAGAGACAGAGAGACACAGAGACAGAGCCATTACCGAAGATCCATATCCATGAGCCAGAGCCAAATCCTGAGTCAGATAGACAGAGCTGCAACCAGAGTCAGACTGAGTCTGAGTCAGGATAATAGTCAGTGTTAGAATCAGAACTGGAGAGCTAAAGAGCCAGGGAGAGACCTAGAGCAAGAGCCAATGCCAAAGACAAAGAACCAGAGTCAGAGAGCCCCAGAGACAGACTCACAGAATCAGGAGTATAAACAGAGAGACAGTAACAGCCAAACCCAGAGTCCAAGAGAGAGAGGGCTAGAACCAGAGTCAAAGTCAGACTAGAACCAAGACCAGAGCCAGAGCAAAATGATAGTGTCAGAGAGCTACATCCAGAGCCAGAGCAGAGACAAAGCCAGAGACAGAGACAGAGACAGACAGAGACAGAGACAGAGCCACAGCCACAGTCACAGCCACAGCCAAAGACACAGCCACAGCCACAGCCAAAGACATAGCCTGATATACAGCCAGGGAGCCAGGGAGCCACAGAACCCTAGAGTCTGAGACAGATATCTAGAAAACCAGAGCCACAGTCAGAGTCAGAACCAGAGGGCCACCAAGTCAGAGCAAAAGAACTAGAGCCAGAGACAATGGCAAAGCCAGAGAACCAGAGGCAGAGAGCCAATGAGCAAGACTCACAGCACTATAGGCAGAGCCAAAGAGACAGACAGGCATATTCAGAGCCAGATCCAGAGCTGAAGCCAGAGGGCAGAGAACCAAAGACAGAGTTAGAACAAGAGACAGTATCACAGGAGAGCCAGAACTAAGGAGCTGAAGCCAGAGAAGAGAACCAGGGATAGAACAAGAGCCGGAGAGACAGAGAAACAGAAACACACCTGGAGCTAGAGAGGTATACACAGAAACACAGTCCAAGCAGAATATAAGGCCAGAGAACAGAGACCAAGAGCCAGTACCAAAAACAAAGTGCCAGAAGCAGAGCAAGAGAGCCAGAGTAGTAGCCAGAGCCAGAAAGCCAGACAGCTAGTGTTGGAGCCAGAGCCAGAGGCAAAACCAGAGGCAGAAAGTCAGAGAGACAGAGAGACAGAACCAGAAGCAGAGCAAAAGACAGAGACAGAGAGACAGAAAGCAAGGGAGACAGAGACAAAACCATAGCAAGAGCCAGAAACACAGCCCTAGCAAGAGTCTAAGAGCTAGGGCAAAAACAGAGAGCTAGAATACCCAAGCCAGAGACTGAAACATAGCCAGAGCCAGAATGCCATATTACCAGAGAGTCAGAGAGCCAGACAGCCAAAGTCAGATAGCCAGAGGCACGGCTGAAGTGAGAGCCTGACAGTCAGAGCCAGGGACAGAGATCCAGATATCCAGCACTAGTG
D3_15 132183606 132198654 15049 TTGGAGTCATATCATAAACTTAACTTCTGCTCCCATCTGCATTGGTGGTCATTAGGCAGGGGGTGGTCTTGTAAAGTGGAGGTGCAGGTTTGTTGGGGGAATAACTTGGAGACACAGGTCTTGTTGAGGATGTAACCTGAAAACTCTGGTCTTGTTGGAGATTGGCCTAGAGAATGGAGCTAAGCTCTGGTTTTGTTGGGGGACAACTTGGAAACTAATGGTGGGTACCAACCTGTTAGTTAACCTGAGTTCAAACTTAGGTCAGGTTCTCTAAAATGGAGTCTGAACTTAAAAGCATTGGCCTCTCATCCCCCACCCACCCCTTTCTCTGTGACTAGAAGGCCCAATCATGGAACTTTCTGAAGCTTCATAAAAACAGTAACAGGGCTATGTCGTTGTGATGTCCCTGATGCATGGGTAGGGGCCCATCAGTGGGAGATAAGAAGGTGTCTTGAGGCTGAATTCGCCTCCTGCTAGCCTGCGTCTTCAACTGCAAATGGATGTGTGATGGATCCACACTGTAATTATGTAGCACAGGGACCCATTTAGTGAGGCTCTAGTGACCCCTGGGTATCAGTGTCTCTTGACTAGGACCCAATCTCAGGGCTAAAGTTAACACCAGAAAATATTTGTATCTGAGTGTTCTTGGTTTTATCTCTATGACGTCCATTTTCTGATAGACTCTTGGCTTGGTTCTCCAGTATTGATTATTGGGGTCCTTTTTATTGGTAAATGCATTAGTCAATTGACAGGCCTTACAGTCTTTAACAATTTCAACTATTTTCAAATTGGCATCTCTAATTTTGATGTGGGACCAGCAGACTAAGTCCTGCATTCATTGGGATCCCATGTGGGAAGAACCATAGATCTTTTTCAGTATGTGTAGACCCATTTTCTCTGGTACAATTAGTTTGTAGTCAGCTGTCCTCTGTCATCTCTTATGACACTGAGACATGGGCAGGTTTTTGGCCCAGATCACAACTTCTGTCTGATCAGAGAGTGTGGGGTCTCCTGGGTCTAGTGCAATCAAGGTCATGAGGGGCCTAATTTCTAGTGGCCACCCACTGGACTGTCTTGCTGCCCTCTGGGATGGAGTTTAGTCCTTTCTTCTGCCATCAGAATGCCTCTCTCCCTATAAATAGCCCCATGGAAATGAGCAGAAGCAAAGACACACCAACCATCTGTGTCTCTTGTCTTTTCTCAGCTCTAAAGCCTTGGTCAGAGCTTCCAGTCCAGCTTTCTTGGCTGACATTTCTGCCAGGAGTAGCTCTGCACATATTACCTCAGTGTTTGAGTCCACTGCTACCACAGCATACCTCTGTCCATCCAGAATGAGGCTGGTGCCATTGGTGAACCATGTGACCTCTGCATCCATCTGAGCATCCACGCAGAGGCCCATCAATCAAGGTCAGGGTCAGGCAGCTGAAAAGTTATCCTGGGGACATTCAAGAAGAGAGGCTGGTAATGAGTCCTTCTGGCATCATCTTTAACCAGGAGGTCACGGCTGCACTGATGTGTAAACAGGGAGGGGGGAGCTTCTGGCAATTGGTGACACATTGGCCATTGGCTGATGCCAGGCCCCTGAGTCTGAACATGCATCCCCCAGGTCTAGGGAGGTCACCTGTAGTTGACAGAGTAAGGCTGTCTTATAGGAGGCTCAATACTTCAAATTTTTAGGACATCTAAGTAATCTTGAGTTCATGTTTTGTCTCTATTGTCACTGGGAAGTCATTTTTTTATTTCTAAGGTTATCCTGTACTTGGAGAAGGGCACATTTGATCTTTAGGACACCCTTTATCCTCTTCTTCTAAGACTATCTGTTTTTAGGTGTCTTCTGTGGTCTTCTGGTTTGGTCTTGGCTGCCAACAGGATCTTTGCTATGTCCCAATCTGTTGCTTCTGTGCTCTCATCTACTTTTCCTCTATAATCTCTCTTTTATTAAATACTCTCCCTGTCACTATCATTATGTCTCTCAAATTCTTTCCTCTTAATCTTTCAGCTCTCTGGAGGTTTTTCTTAATATTTGGTGTTTCCTTCTTAATAAAGCTAGAGTGATGGCTGCCTTTGTCCTTGGTGATTTAGAGTCCATGAGGGTGTATTATCAAAATGCCTCCATTAGCCTCTCTAAAAAAGCTGTAGGGCTTTTATTTTCTCTCTACCTAATATTGTACACTCTGGCCCAATTTGTGAACCATCTTGTGGTCACCTTGTGATCTACCAACTGAGTCTGACAGTACACCAGGAGCCTCACTTACCTTCAATGGTATTGAAATCTGGGTGAGTTAGGGGAAGGCAAGCATATTGGAGAGGACACACTATGGGGCCAGGAACCAGCTTCTTGGCCTCTTCTGTAGTGAAGGGAACCTGTAGAATGTGATAGAGGTAGCCTATCTCATGTTAATAAGTCCAGCAAAGTCAAAGTCACTTCATCAAAGTCATCTTGTCAAAGTTATCTCATCAATGTCACCTTGTCAAAAAGTCAACTTGTCAATGTCATCTGTCAAAGTCATCTGTCAAAGTTGTTTTAAAATAAGAAACACAAAGACAACATACAAAACTACAAAAAAAAAACACCTCCCTATTTGTCACAAAAACAAAAAAACTACCAGAAAGTGGTGCTCATGTAAAAGACCTCCCTATAACACCCAAAACACAAGTCCAACCTTTTCTGTCTGTCAGGCAGAAATGAGAAATACCAGAAAATGGCGCACACCCACAAGAAAAGAATGAGATTGGTGTTTTTCTCATGGCTCCCCTGAGCCCATGCCCACCCCAGACTCATGCTGTCTCCTGGCCCCGCCTTAGCCACAGCTTCCCGATATGTCTATCCTAGCTGAAACTGGATACATACACAATCAAGACCCTGTTCCCATTCGGGTAGATACCTCTGCTCCCCAAGCTGACATCCACGCAAACTGAGGCTCGGGATGTCTCCTGCCCCCTCTGTAGCCATGGTTCCCAGATATGTCTATCCTAATCGAAACTGTCCACCAAAACAAATTCAAGGCCTTGTACCCAGAATGATGTGACACGGTTTAAGATCAGTGAACAGAAAAGGTCACAACTAGAAACAAAAATCAACCTAAACCAGAAAAAATACAGCCTGTAGTAACAAGAATCAACTCGAATGTGGTGGTCACAAAAGACAGGTGTGGAGCCAGTCAATCTGTGAACAGAGACTGCAGGCAAGCAAAAAGCAATACCAGAAAAAAATAAAGTATGTAGTAAGAAGAACCAACCAGAAGGGGGCGCTCAGACACATGACAACCTTAGTATTGCACAAATATTGACTCAGACATGGAAAAATTACCATGGCAACATAAGTCATTTATCTTTTCACGAGTTTTCGTTTCCATCTGTAATCACAGAAAGCTCTGTTTCAATCACAGAAAGCTTCTAACATTCAGACAAATTAGCCGCCACACAAATCACAAGCTTTTGCGCCACTGTGCAAGCACTGGCCTGTTGAGACCACATAGAAAGCTTCTGACACTCAAGATTCCTCAGCGCAGCACAAGCCTAGAAGAATTACCATGGCAACACAAGCCACCAGGTTCCACACAAATACATCATCTCAGGACAGCTGGAAGTGTTCCACATTTATCTTACACAAGCTTGCTTCCATTCTGCTGTGCAGATCTCACACAGAAAGCTCAGATACTTCATCTCTCAGACGGAAAAGGCAGGAAGATTAACACAACGTTCCAAACATAAAACCACAAATACAGCCGGGCAGTGGTGGTGCAGGACTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGTTGATTTCTGACTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGACTGTACAGAGAAACCCTGCCTTGAAAAAACAAACAAACAAACAAACAAACAAAATAAAACAACAACAACAAAATATCCCCACAAATACCTAGACAAGTTGTGCCAGCTTCAAGACCCTCCAGATAGTAGTCCTGTGCGTCATGGGGAACTTCCTGGCCAATGCACCAAAGTGTTGTGCCAGGGATTGTTTGTGATCCCCCCACCCCTACCCCCAAAACAGACAGGTGCCAATCTGATGCAACTCACAGCAGGGTCTTTATTCCATTCAATCTATCTCACCCTCCCCCGCAACACACCACCATCACACAGGATGGTTTTGGTGGTGGTAAAGCCCCAAATATAGGGCAAGGCTTTATAGTAAGCAGCAAGCAGGGCGTACGTGTGCAAGCATCTAATTGGAATGCCACTATAGCCTTCAACATTATTGCCTGGTGTTGAGAGTCATATCATAAACTTAATTTCTGCTCCCCTACTCATTGATGGTCATTAGGCAGCGAGTGGACCTATAACCTGGGGTGCAGGTTTGCTGGGGTAATAACCTGGAGACACTGGTCTTGTTGAGGCTGTAACCTGGAAACTCTGGTCTTGTTGGGGATTGGCCTAGAGACTGAAGCTAGGCTTGGGTTTTATTGGGGGTCAACTTGGAAATTAATGCTAGGTACCTGCCTGTTAGTTTACCTGAGTTCAAACTTAGTTCAGGTTCTCTAAAATGGAGTCGAACTTAAAAGATTTGGCATCTCAGTAGCAGAATTATGATTCTGTATTCTCTTTTGTAATTCAGTACTGTTGCAAGATTTTCCCTGTCCAATCACATTAGGGCAGTGGGAGGCCTGTTATTGGACAGGACATGGGGAGGTGGAGCTAAAAGTTGCAGAGAAAAAGAGAGCATATCTGAGTAGGGTGAGAACCAAGATGGCTGTGGACATGTATCCACGTGGTATTTTCCAGCCACAGGTAGCTATGATTTCACAAGGTTAGAAATATTTGGATAAAGCTTTTATCATTATCAATTGTCTTTGAAATTATTGTATTGGCTTCTTGTACATTGTGATATTATTGATACATAAATCTCATAGGTTAACTAAGGGTCTTGATTCTACCGGGGTACTGGGTATTATGATATGGTGGCTGACTGTGGGGTGCATGGTAGTGAGTGGAACTTGGAGGCCTCCACAGGAGAGATGGCTCAGCAGAAGCCCTGTGGTCCAGCGTAGCTGGAGAGTTGGCGATTCATTTTAATATTTCTCATAACACAGTACTCTTCTGACTTCATAAGTACTTAATTCTAGATAGTCTCATAGATTTTCTTTGTACCTCACAGGCCCCTTATGAAGGAGCTAGAGAAATTACCCAAGGAGCTAAAGGGAACTGCAACCCTATAGGTGGAACAACAATATGAACTAACCAGTACCCCGGAGCTCTTGTCTTTAGCTGCATATGTATCAAAAGATGGCCTAGTCGGTCATCACTGCAAAGAGAGGCCCATTGGACTTGCAAACTTTATATGCCCCAGTACAGGGGAACACCAGGGCCAAAAAGGGGCAGTGGGTGGGTAGGAGATTGGGGGTGGGTGGGTATGGGGGACCTTTGGGATAGCATTGAAAATGTAAATGAGGAAAATACCTAATAATAAAAAAAAAGAATATTCCATGCCTTGATGCCTGTCCAGTCTACCATACTGAAAACTGATATGCCATTGCAGTGAAAAGCGAGAACCCAGTTCTTTTTTCAGATACTTGGGGGAAAGAGACAAGGGACATGGCTTCAGGTGCATCATAAAAGACACTGATGTTTTCTTGCAGCTCAAGAGTTTATTAATGAAGGGGATTGGGCCATTTGGAATTTGTACGGTTGGCCGCTAACAGTTGCATCCTGAATCTTAATGAGGAAGAGGGTCACCTGCAGTTGTTGGCCATGGGTGATGTTATCCTAGGAACATAATGAGACCTCTCTTTGGATTCCTGGTGCCAGGACTTGGGACCAGCATTATTTGATTGGCTTTTCTTCGTCTTCATCATTTTGTGTTGAGATGATGTTTTTTAGGATTTCAGCATAGTACGGGCTAAACACATGCTGGTTGTGGTGCATCAGATTAACAAACTTCCATCCTAGTTCTGCTAACTCTGGCTCAATGAAAAGAAAGCATCCAGGGAAGTCTAGAAGAGATTCTTGCATGCCACGAATCAAACAGCATTTAAGAAATAAATGCATCCGTTGTTCTGTCATAGGGGGAAAAAAGAAAGTAGAAATGGGTTTCAGAAGAAACTGACAGTTCACTGTCTGCTCATCAAAGCTTTACGTTTTTTTCACTGTGGTGGTTTGAACAAGATGGCCCAATAAACTCATATGTTTCAATGTTTAGTCACCAAGTAATAGAACTTTTTAATGAGATTAGAAGGATTAGGAGATATGGCCTTTTTGAAGGAAGTATGTCACTAGATATGAGTGTGAGGTTTTAAAAGCCCATGCAAGACCCAGACTCTCTGCTTGCAGATCAGGATTTTGCTTTCAGCTACTTCAGTACTACCCCTGCCTGCTCCCGTGTTCCCTGTCATAATAATAGACTATACCCCTGAAACGGAAAGCAGTCCCCCAATTAAATGCTTTCTTTTATGAGAATTGTCTTGGTTGTGGTGTATCATCACAACAATCAAACAGTGGCCAAGACATTCATTTTTTCATTTGTATGTATTATTTCAGTTCTTTTTGGAATAAAGACATACCATCAAACACCAAAAGATAATGAATTATCAAAAATGTTAATCTAGGACATGAGGTGTAACTTAATAGCCAAATGCCTGGAGAGCATATGCACGGTATGGAGATTGATCCACAGCACATACCCTCAACAAACACACAGACTCAATGTTGAAGTCAGAAATACAATTGTGGCTTTTGGTCATTGGATTATTTCTTAAAATGATTTTTACATGTTTATAATTGAGATGCTAGTATGTATTTTGCCTAACAAGATCATCATAAACATCATACCTGAAATTCCAAGAACATATTTAAGCTGATATACTAAGTTTAGACCCATATACACAAATTAGAGTATAAGAAGTTAAAAGAAAACTAGCCAGGGTCTGGAAGTTAAAAGCACTGACTTCTCTTCCAGAGGACCTGAGTTCAATTCTCAACAACCATATGGTAACTCACATCCATCTGAAATGAGATGTGATGTCCTCTTCTGGCATGCAGATGTACATGCAAATAGAATACTCTTGCATAAAATAAAATCAGAAAAGTGGCCAAGTTTACTGTGTCTATTCCACAATATAGTATGTTTTATTTTATATTGTAATTCAGCATTAAACCCAACAATAGGAACCTGTATGGCACCTTAAATGCTAGGGTAGGGACAGTTTTCCAACATAGGGTTGTGTAGTGGTTTTATCCTCTCTCTTCTCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGTGTGTGTGTACTGTAATTTATACATTATAATGTATACATATATGACTATATTTTGATATATGACTATATTTTGTTTCAATAATACATATTATAGTTAAGAAAAGTAAAGTGCTTTCCAAATATCATGTCTTACCTTTTGTTTTGTTTTGTTTTTGTTTTTGTTTTTAAGTCAGGGTTTCTCTGTGTATCCCTGACTGTCTTGGTCTCTGTAGACCAAGGTGACATTGAACTCACTGAGATCTGCCTGCCTATGCCTCCCAAGTATTGGGATTAAAGGCATGCAACACTGTAAAAGTAACTAATTTACCAGATGTAATTGTCATCTCAGAGGCTTACTGCTTAATAAGCTCACCTGTCTAACTCTTTCTGAACTCTGGCAGGCTGATTCAACTCAGCTGGTCTGGTCCAAACTCCTCTCCAAGCGAACTGATTGAATCTGCCTTCTCTTGGCTTCTGGCTGAATTGCTCTCCTTGGACTCAAACTAACTCTGGTAATTTGTTCTAATTTTCTGTCCTTTTCATACTCTGGCTCCTTTTGTCTTCACCTGCAACCTGTATCTGTGAAATTCTCCTGGTAAAAATTCCTCCTCCTCCTTCTCCCCCTCCTCCCCCCTCTCCTTCTTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCTCTCTCTCTCCTATTGCTCTCTTAAGTAGCCTCTTTTTTCTGTCTGTTAATGTTCACATGTGAGTTGTGTATATCCCACCTCTGAAGATTCTGTCAAATCGTTCTCTTATTCATCACTTTGCCCTTCAACTAGAGGTCACTTTCATACATGGCTGCTTCCTTTTATAAACTAACTTTACCTTCATTGTTTTGGATTAAAGGTGTGTGGTAAGGATATGTCTGCATCACACATACCTATAGAGTGATCCTTTGATGTGATCCCTTGGCAAAGCACCTATGTTGCTGGATTACAACTCTTCTACATACCACCAATTCCTGGCTCCATTTAAAAAATGTTCAGTAAGTCTCAGAGCAGAGTCTAACTCCCACCTGGCATGTTATTGATATTTCTTAACCTCTTTTGGGACTCTTACCAATGATGATACGAATGCAATTCTCTTTCTTGGCAATGTTCCGCAGTTGTCCTACAAGTGATGCCCTGTTTTCACTGCTCAGAGCAGTTAGGCCTGTGTCTTTGAGACCTTGATGGATTTCCTGAGACACTTGTTCACTCACACTTAACATAGATTCTTCTGGCCTAGAAAGTGAAGACAGAAAAATAGAGTTTCTTTGATATAGGGCCAAACCCAGAAAGACCCATACTAGAAGCTAGGCATTCCCAAGGGGAAAAATGTGATTCTCTCACCATCAATAGACAATCTGAATCAATGAGCATGTAAAATAATCCTGTTATTAACCTTAAGGTTCCAAATTATTCATAGATATATTACTCTATGCCAACTATATGGGACAATATATATTGGACATGGCTTTGTTTACATTCCAAGAGCCATCCAATATTCTTTTAAGTAATGAAGATTTAAATGTTGTTGAAAGGTATTCTTAGATATTTTACATAACAGCCTTTCCTATTTTACCAAAAAATGTAAATAAATTAATAGTACTAAATTTCTATGTCCTCTTTCTACTTTAATATGGTATCTCTGTATGCCATGGGTAAAAATAACAAAAATCCGCTGTCCTTTGATGATATATATATATATATATATATATATATATATATATATATATACACTCACTGTTAGAAAACATAATAATGAGTAATTGTAGCAAAGTACCCATCTTATTTTCCTTCTAGGTGGAAAACTTTTTATTCTTCTCTATGAGAAACCTAAAATGCCAACATTTTTGCACCTGGTTCCAAAAATCCTTTGTGTGAAAAATAATGGAATATTATATTGCCTGTCTATGACCTAAATGCCATATTATATTCTAGTCTAAAAGAAATTTATAACACAGCTGGTAAGTTTGCTCACTTGTTAAAGTGCTTGCCAAACAAGCATGAGGGCCTGAGTATAGTTCCATAGTCAAAAGTGATGGATCATGACTATAATCACAGCACTAAGGAGGCAGACACAGGTGGCTTACTAGAACTGGTTGGGCAGCCAGCCTAGCTGAATGGGTCAGTTTCAACTTCAATGAGACACCATGTTTCAAAAAATGAGGTAGAAAATAGGAGAAAGATCCCTGTAGTTTACCCCTGGCCTTTCTTGATATGTGTCATGTGCACACTTGCACAAATGAACTTGTATGTACATGTGCAACACACACACACCAGAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAAAGAGAGACAAAGGCAGAGCGATCAATGAAGCATACTTGGAGCTAAAATCTTCAGTTAGAGCCTTTGTTATGCACTTCAATTTATCCACAAATTCTGGTGAGCTGAATAAAACACCACAAGAGAAACTTCTTGCCACCAGTAAAACTGAGGCTAGAATAGTCAAGTGGCGAAATTTGGATCTCATAGTCTGCAGTCGTGCTTTATCCATCAGCAGAGTCTGTTTAGGCAAAAAAGAACAGACTGTTAGAAAAGGAGAATTGTAATGGGACTTCTAGGTAATGAACACAGTAAATGCCTGAACACACATCCATACCTCAGGGAATTCTTCATTCCCATGGTCCCTGAGAACAAGATTTAGGTACCCTTGGTATAGCACTGTTGTAAGACTGGGTGGATCTGGGTCAGGCTTTGGAGTAGGAAATGAACTTATTACGCCAGAAGAGATACATGAAGTGACAGATAAACTTGAAAGTGATGTAATAATCTCTGTGGCAGCTCTAGTCATCCATTTTGTGGTGCAATCAAGTCGATCTATGGAAGAGAAAGGAACATGTATGTTTAAACTCCAAGGCATAACGTTAGAGACTTTGGAGATAGTTTCTTGATGAAGAAAACATTACTCTTAAAAGCTGGAAGTGAAATGGTTATTTCCTTCCAGAATAGCTTTCATAGTCCAACAAGGCACTCTGCAGACAGCTGGTGGCAAACTCCAAACCAACCTGTGGACCTTGTTTACTCCAATACCAATATTCCAAACCAGATCTACTATACGAGCACGACCAGCATGGAGGCAATCAACAACTTTCTAGGAGGATGTGAGGCCCACTCCATAGGGAGGAGTTCATGCATGGCTTTGTATAACTGGCAAAAATTCACAGGCAAGGAACTCATACTCCTTAGAGGTGGACCTATTCATCCATTTTTCTAAATAAAAAAGGTTGTCAACTTGCCTCTATGTAATTATGTTTATATGCACAGATATGTGTTCTTTGCAGCCTTGAGCAGATAATGCTTCTTTTTGCAGTAAACACCAATTAGTCCAAAGATTGGGGCTTCTCAAAGTTGTAAGAATAAGCAACTGTTGTACACTCTGCTCTAAACAAAACATCTAGCACACCCTCTCCAAAGCTCAGGAAATATAGAAGAGGAGGCAGAAACAGTACGGAAGTGGGATGACAGACAGGAGGGCAATGGATTGTTATGTTCTGGACATGACGTGGTTATTGCAATTGTTTTCTCAAAGCAACTGAGATAGTTAAGCTGAGTCTGCATAAGATTGGATCTGTCAAAAACCAATCGTGGGTAGGGGAAGGGCTCATGGGGCTGCACTGTATTTCAGGAACTACTTTGATGGCTACTGGAGGAGGAGTCATTCAATGATCTACCACCAAGTGAGATGCTCCTACTCCAGTGGATGGTTCTATACCCACGATGATGCAGGTAATCTTCAAACTCAGGTGGTGATAAAACAAAAGTCATGAAGGTAAGAGAGGGGATTCTTTAAAAGGGAAGTTGTGGTAGTGGGAGGGCATAAGAAAGGCTGGGCTTGAGACTGATCAGATTATACCATGTACATCTATGAAATTATCAAAGAATAAATTTATTTTTAAAAAAGTGATTTGCATAGGCCTTTCTGTTTTGGAAAATTTTTCTGCTCTTAACACTTGACCCAAGAAGTAAACTTTTCATTCAAAATAAAAAACACTGCAAAAAAATTAGAAGCCAAGTTCCCCCTCTAGTTAAATTTTAAACATACCAGGCTGTTTGTCAAGGAGTTCTTGAAATTTAGCTCGTTCATATTGTATAGAATGTTCCTGTAAATATGGTCGAATGTTCTTAATAGTATAGTTCACCATATCCATTTTCATCAGGCCTAAAACTTGGAAGATACCCCTGTCACAGACAAATACAGAACATTTAGTTTCAAAGGAAATCTGGTGTTCAAAAGTGGAACTCAAGGAAAGAACAATAAAATCACAGTATTGAGAATAGGAAAGGAAGTGAAAAGTACTGATTTCATTTAAAAGTAGCTAGTATTTAAACACGTAATCAAAAAAAATTAGTTGCTTTGAGTAAGGGCCTAATAAGAGCTGTCATGAGGGCTTCAGTTTTACAAATAAGTAGATTATATTATAAAAGGGCGTGAGGCTCAGAATTTAATGTTGCTTTATTTGACCGGATAATGCTATTTATCATGTTATAATGTATCAAGAATACTCTCACCTCCAGAACTGTAAGAAATACATCTATGCTTTTTAGAAATTACAAACACTTGAGTATTTTCCCTGAGCACAACAAAATAAACTAAGGGAGAAGAGTACTATTGAAATTTTGGACTGATGATCCACCAAATACCTGAAAATGTAGAAAATGGGCAGAATCTAAACATTTAAAGAAACAGGCTATTAAAAAAAAAAAAGGTTTGTTACAGGGCCCCCAATGGAGGAGCTAGAGAAAGTACCCAAGGAGCTAAAGGGATCTGCAACCCTATAGGTGGAACAACATTATGAACTAACCAGTACCCCGGAGCTCTTGACTCTAGCTGCATATGTATCAGAAGATGGCCTAGTCGGCCATCACTGGAAAGTGAGGCCCATTGGACTTGCAAACTTTATATGCCACAGTACAGGGGAAACAGCAGGGCCAAGAATGGGGAGTGGGTGGGTAGGGGAGCAGGGTGGGGGGAAGGGTATAGGGAACTTTCAGGATAGCATTTGAAATGTAAATGAAGTAAATACCTAATAAAAAATTGGAAAAAAAATTTAAAAATCAGACATGAAAAAAAAAAGGTTGTTAATGTGACTGTGGTGTTAAGGATGACTTGAATGAGGGCTAAGAAGGGAATAATATTTGTAATATGTTTGATGCTTTTGGACATTACTAAAATATTCATTATCAAAATTTTGGCAGAAATATAGATACTAAAGACCAGTATGATGAAGTTTCAAATTAAAATGAGAAGTCTCACTGGAAAACAGATAGCCTTACCTTATAGGAAAGTAGCTTAAAAGTAGCCCTTGATATGAAGTAGCAAATACTTTGAATTGTGTCCAAATTCAAAGTGTTTTACAGAATGTAAGTTTTGAGAACAATGTCCTAAGATATTAGGCAATAGAATTTTCTCAGCATGATACTGAAGGAGTTACCTGGCTAGTTTTAATCACATGTAGAAATACATGGAAAGAAAAAAAAAGAAAATTGAAACATAAGGAGAGTAGACTAACTTGAAAAATTAAAACTGACAATGTAAATAGTAAAAACCCAATAGGATCAAACCAAATATACCTAAGTAATTCCTTTTCTAATGAGATGAGTGTGGACAGAAGGAAATTATATGCTGGTCACACAACAATGGGAAATACACCTCCTAATGAATTTCAAAGATCTTAGAGGCAAGTAAGAACCTTTATTGTAAGATTATAGAGACATACCTACAGGATGTCACCTTTTGCTTGCCAAGAATACCTAGAGACTCTGCTCCCTGAATTTCAGTATAGAGCCCCTCAGCCAGCACAACTACCACAATGCAAACAAGCTCAGGTGTATATTAGGTTGGACTATGACTCCACTCAAAAAGTCTGGAAAACACAATGGTAAGCCTTAATAATCCCCTAGTAGCACTAATTATGTAAGCACACAGAATACATGAGCTACAAAGCAATAACAGTCTCCATCTTGGTTTCAAAAACATATGTGAAACGATCTGGCAGCCTAGGCAAAAGTCTGCCATAGAGGTAGAGTTCACAGAGTATCCACACACATGGCAATGTCTAGTGGAGCTGCTGGGCAGCCTCCCAAGAGTCCAGCATTATAGAACATCCCTGTACGGAATGCTACAAGTAAGAGACTCAAATTCATAATCATGCCTCATACAGGATGAGCCCAGTAAAACTATGTGTGTTAGCCTGACAAAAACTATTAAAATGCCTTTTTTTTTGGAAAAACTCTTTATGAAACTGAATAAGACATAAATAAATGCACCATTCAGGGACCAAAGGCCAAAGGGTGAGTTGGCAGCAGAGCTAAAAAGAGACACATAGTATGACATTTCAACAACCATTCTAGAGCTCTTTGCACTGTGCCTACCCTACTACATTTGGAAAATGGTATTGATAGAAGGAAAATTCACCACAGCACTAACAATTTTCTATGAAAGGTTAGAATGTGGGTGATTAATTTCCAATTCATATAATTTTTCTTTTCTATTTGTTTTAGCTGTGAAACACTGAAAGTCTTCATTTCTGTTTTTGCAGAGTTCAGACTGGGAGAAATAGAAGAACACATGAGCTGGGGTGGACGGAAATCTGTAGAATTATTACTATACTTAAATGCCTTAAGAGCATTTCATGAAATGTATGGAATCTCAGTGATCATTTACATGGCATCAAAATCAACCAGAGACACTGACTGCCCTTACCAAGTAAAAGGGCAGGGAATAAAAAGGTAGTGCATCTTAGCCTGGGACATAGAATATGTTTTACACACTGTGTAACAGCTAATGTGGAGACTCAGCTGGTCAAAGTGCTAAAATAAGTGATTAATGAGTGCCCAGACATAAAAAAGACACACATATCATCACTCCCATGGTTCAGCAGGCATTATAGAAGATAAGATGGAAAGATTGTTAGAGCCAAAGGATGAAGAGGAGTAATAGAAAATGCTATCTTCTGGATTTGGCAAGGCCATTACAATCACAAACATGTAGCAGTTACAGATACATGGACACATACTGCATAAAATCAGAAACAAACTTCAGAATAGGAGTAATTGGGAAGAAAAAATGGATCAGCAAGATTGAAAGAGGGATGATAAAGAATAATATGCATTGTTTATAGTATGAAATTCAAAAATATTAAACAAGAGTGGCTGGAGAGTTTGCTCAGTTATTAATGGCACTTGTTGTTCTTACAGAGGACTGGATTCATTCCCAGACGTCACATGGTGGGTTAGAAATATCTATAACTGCAATTCCAGAGATCCAACATTGCTTTCTGGCCACAGCAAGTACTAGGCATACACATTGTGCACATATATTTTTTTGCAGGCAAGATGTTCATAAACATAAAATAAAATAATTTAAAATAAATAATTTGTTAAAATTTAAAAAGTAAAAGTAGAAAATAGAAGTGAAAACTTATGTTAGCCATGTCTTCAGAATAAGACAGAAGTCTATTAAAATATTTGGCTCAAATTTTCTTTCATGGTACATAACAAGGTAGATTTCAGGAGCAGAGTATGGTATTGTTGGAAAATAGGTAACTGCTCAAAAACTAGTAAACTTCAATCCCAGCTGTGAGTTACTGAGAGAGTGGAAAGCTTAATCCAATTATCAAGTTTATAAATGAAGCCTTTAGACCATCACCAGGATTACACAAGAATGGCAGTCTAGAAACCGCCAAGATTGAATAGAAATGATTTCTCAAATAAG
GF3 -1 -1 1 CTGTTCCTGGCTCTGGCTGTCTGGCTC
GF4 -1 -1 1 CTGGTTCTGACTGTGGCTGTGGCTCTCTG
EX52 -1 -1 1 GATGGCCCTCATGTGCGCACCAATTAGAG
EX32 -1 -1 1 GAAGATTCTGACGTGGTAATAGTAGTGCC
GR3 -1 -1 1 CAGGCAAGGAACTCATACTCCTTAGAG
GR4 -1 -1 1 CAGGCAAGGAACTCATACTCCTTAG
Comments:
Created
Target: 0402Ensembl ENSMUSG00000058252 -- 132198922 -- 132199142 ENSMUSE00000545942 -- ENSMUSE00000545942
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 7 oligo candidates from u/d regions
AOS Produced 13 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:X:1:166650296:1, start 132198921: end 132199142
Found 4 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:X:132204642:132205641_932 G3:X:132194422:132195421_891
ACCEPT (0) gap pair G5:X:132204642:132205641_932 G3:X:132194422:132195421_745
REJECT (1) gap pair G5:X:132204642:132205641_932 G3:X:132194422:132195421_634
ACCEPT (0) gap pair G5:X:132204642:132205641_927 G3:X:132194422:132195421_891
REJECT (2) gap pair G5:X:132204642:132205641_927 G3:X:132194422:132195421_745
REJECT (1) gap pair G5:X:132204642:132205641_927 G3:X:132194422:132195421_634
ACCEPT (0) gap pair G5:X:132204642:132205641_922 G3:X:132194422:132195421_891
REJECT (2) gap pair G5:X:132204642:132205641_922 G3:X:132194422:132195421_745
REJECT (1) gap pair G5:X:132204642:132205641_922 G3:X:132194422:132195421_634
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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