High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
 
       
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by wy1
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 50028
Chr: 18
Strand: 1
Status: Ready to order
BACs: RP24-426E19 : RP24-510M7 : RP24-144G14 : RP24-510G7 : RP24-511D21 : RP24-372A6 : RP24-238O7 : RP24-536E13 : RP24-378G19 : RP24-378G18 :
Oligos on GRCm38:
Type Start End Length Seq
G5 65244202 65244252 51 ATCGATAATTTAATGTGACTTATCAGAAAGAGAACGAATTGTTTACTTTT
U5 65248626 65248676 51 GGTTTGTAAGAAGTGTCTGCCTCTTCCTGGAATTCAAGCCTTTTAGGAAA
U3 65248788 65248838 51 GTCTGTGACAGACAACGGAGGAATGTGAAGGGAAGGTTAATACCCTTGTG
D5 65248994 65249044 51 TTGTGCTAGAGTTCTCCATTGTGTGTAATGTATGAATAAAATCTGGTCCT
D3 65249068 65249118 51 ACTGACGCCTTCCCCTCGTGTGCTATTGTGAAATGAATAATGCTATAAAG
G3 65252163 65252213 51 AAAACCCTGTGCTTCACGGCTGAGTCCATGTTGAAACTGAATTAAACATA
Oligos:
Type Start End Length Seq
G5 65369572 65369621 50 ATCGATAATTTAATGTGACTTATCAGAAAGAGAACGAATTGTTTACTTTT
U5 65373996 65374045 50 GGTTTGTAAGAAGTGTCTGCCTCTTCCTGGAATTCAAGCCTTTTAGGAAA
U3 65374158 65374207 50 GTCTGTGACAGACAACGGAGGAATGTGAAGGGAAGGTTAATACCCTTGTG
D5 65374364 65374413 50 TTGTGCTAGAGTTCTCCATTGTGTGTAATGTATGAATAAAATCTGGTCCT
D3 65374438 65374487 50 ACTGACGCCTTCCCCTCGTGTGCTATTGTGAAATGAATAATGCTATAAAG
G3 65377533 65377582 50 AAAACCCTGTGCTTCACGGCTGAGTCCATGTTGAAACTGAATTAAACATA
G5_U5 65369572 65374045 4474 TCGATAATTTAATGTGACTTATCAGAAAGAGAACGAATTGTTTACTTTTAAACCCTGGATCCCATAAAGGGAGAGTGCACAAAGGCCTAAAAGCCACAGATGCTCGGTGCTCTCACGCACGCAAGGCTGCCCTCAAACCCTCAGTAGAGAGAAGATAAACACTGTCGGAGGTAAGTGCCTTCTTCCAGGGCCTACAGTGGGAGTGAGGCTGCCTGCCGAGGGGAGAGTGAGTTGCGGGCTCAGGTGTTTAAAAGCCGCCACACAGCTCCTGAGCAACACTGGGCATAAAACATTCCCTAATTGAATTTGGTCTGCCATGATTATTCATTCTGTTCAGAAAGCCTGAGCAAAACTGGACACTTTCTGAAAGCTTCTTTATACTCCCTAGAGAGGGGAAGGGGAAGGGGAAGGCTAGAGACATATGGAAGACGCAGAGGCTGGCTTGTATTTCTATCCTTGGAGTGGCTATGGTAAGCCATGTCTCATTTAAGAATGGCCTGGTTATCAATAGCATCAGGGCCTTGGATGAAGATTCTACCTGTCTGTTTAAAATGGGGGTTTGTTTGACAGGGGCTCCTCTTTGTGTGTGCTTCACCATGCCTTATTCTTATTTCAAAGACACCTGATCCCAATTCTTCCTCGTGTGTGTTCCAACTTGTACTGTCCCCTCAATGATGCTAGAAATAGCTGTCCACCTGCATAGAGGTGAAACTGGTCCCTAGGAGTCCCGAGCTTCGGCAGCCCTTGATTGAGAGTCCATCGAGCCTTTAATGCTGAGAACCTGGCGGAGGATTCTCTCTTTGCCTCTGGTTGGTGACTAAATGGCCGATTTGATTGATGTATGGATAAGGAACTTTTCCAGATTTCTCCCTTTCTAAATGGGTTTCCTTGAAAAGAAAAGTGACTCTGTTCCCTGGTGAAAACAAGAACATTCTATTTTAAAAGTTCTGTGTGCTTTCAAAGATGACAAGACTCGGGAGATATGAACTGACTTTCTTTGTCTAGACTGTACTAGCCTAAAACAGCCAAATGGATACATTTCACACAACAACTTCACATGACAGTAAATGCAGGTTTGCAATTTTTTTTTAAAGCATCTCTGCTTTTTGCTTTTTAAGAAATCCTTTTGTGGTATTGATGGGTTATTTTTTGCAGCAAAGTGACTCCACATAGTTCCCGAAGCTCCCCCTTCTTGGTCCTGGGAAGCTGTTTGCAGGATGTTCTTCGCTCCTTTGAACACGCTGAAAATACAGATTCGTGATCACCTGGTACTTGTGAATCCACCACATCTTCCTAGGCGCTGTGGCGCGCGCTGATGCATCTGAGGCAGGTTACGCTTTGCCAAGGCCTCCTCCTTAGAGACCGCCCACTCCCACTTAGGAAGAGAGATAGATGCCTGGCATTAGACAGGGAGCAAACGGAGTTTAACTTGTTTTTCATGCACGGGGGCTCAATGGGGAAACTCCATTTGGAATTGTCCACAGCCCCTAGAAATGTCTAGAAATCCCATCCTCTTGGAGAAGGGGAGGACAGGACACAGAAATCTTGTTTTAATAGGCAGACACTAAGGGACCCTTGTCACTCAGCCTCCCTTAGGGAGGACTTAGGAAATAAGGCACTCTGACTGCTAAATGGTAGGAGGTGTGTGGGTCTGAGCTCAAGAGCATGAACCCCTTCCTGCGCTACGGAAACCCTGCTGGCATTTGGACACATCACCCTGATTAACATCCAACACCATTGGTCTTAGTCATTGCTTTGTTGATGTTGTTTGTTTTTTTTGGGAGGGTGGGGGTCGGGGGATGTTCATAGAAAATAACAGATGCTACCATTTTTCCATCCAGCATGTGCACTTTCTGTTGGATATTAGCTAGTACAAGTGAGCTTCCCAGTGTTCAGTCTGAAAATTCTAAAGCCCTTTTAAGTCTGAAGAAAGGAAACAGACAATGTGGCTTGGACCCTAGAACGAATGGCTTTGGCTTTTCTTCTCGGTGATACATTCATTAGTAAACAACATTTGGAAGAACAGGAAAATATTCTGCTGACAGAATTTGATCACTTGGTCAAAGAAAACACAGAAAAACAAAACAAAACAAATGTTTCAGTAGTGTCTAAAAATTGTTCAAAGGGCCCTAATAAATGCGAATTTATTTCTTGATATAATAGCATAAAGTTAACTATGCTGCATAGTACTTCAGTAATTATTTGCTTAAAAAAAATTTTTTTTGACCTGAAGTCTCTCTCTGTCAGCAAGACTGTTTCATGGTGGCAAATACATCTGTTTGAGTCAGATAGGTTCCAGCTTTCATATTTACGAAAAGTGTTTACAAGGAGGATTTATTAAATGGGGTTATCCAGAAGGAGGAGGGAGGACTTTGAAATTTTTAAAATTCTCATTATTTTTCTCATTTCCAAAGCACTGTGAAAAAAATTGTGAAAATTAATAAATTCAATGAAACAGAGGTAAAAGTCCAACAGCAGCTCCCTTTCCACCTGGTCGAGCGCTACAGTAACATGAACGCTGTACAGTTCTCCCTGAAAAGGGTCCGAATCATATGCACTGTTTTGAAAACCACTCCTCTGTTGGAAATGAAATCTTACTTTGTTTCCAAGTCAACAACAGCCAACTTTTTACCGAGTCGATGATTGCAAGGTGAGGACCGTGGTCCAGGTGAGTGTCGACCATTTCCCAAAGCATGAGCCATGTGCAACCCTTTATTTTAAATAATGTCACTATGAATTAGCTCTTATTTACTCATTTAGTTAGTTAGTTAGTTAGTTTACCTTTTCCTTGAGATGTGTGCTTGATTCATGACCTGCCCGAAAGGCAGAGAGGTTAGGGGAGGGTTGCGTGTAATATGTTGGTTGGTTCCCTCCTGTTCTTCCTCCTCTGTCTTTTGCACATCAAGCACTGAGAGGTCCTCAGCCCCTACAGCACTAATGAATGCCTCACCGCTCCTGGTCGGCCTTACTACACGTGGGAAGGGGGTTCTAGAGACCTGCTGAGATGGTCAGAGCATCCCCGGGAGGGGGTGAGGGATGAAAAAGAGGTGGGATTTCAGTCCTAGGTCCTCCAGGCAGAGAAATAAATCAGCATAGCTGGTCTCTTGGAAGGAGTGGTGGGGAAAGACAGGTCATCACCTACACACACACACACACACACACACACACACACACACACACACACACACACACACTCTCGAGGTCCCCCATCATTGCATGGCCAGCACAAAGGTTTTAGGGTGGCGCTGGGTGTCAGCAGGAGCTTCCTGGTTTCACAGAGGAGTGCTTGTCTAACACTAGGCTGAGTGACACTCTTTTCCTGCAGTCTGCTGGGGCCACAGAGGAACAAAGGAAGCCAACTATTATATTTTATTTTTTAATTTTTTTTAGTGGACAACAATCCTGTCAGCACCATAGAAAAGGAAAGGGGCGAAGAGGAGCCAAAGTGGGAGGGAGGTCGGAGGTCACAGAATGCTGTTCCTGCGGCATCTGTCTTAAAAGGGTGTCTAAAAGGTGTCTTAAGGTGGTCTCTATGGGAATACAGGATAGAAGAATGCGGGAGGACTCGTTTCGCTTGTTACAGTTTAAGCAAATCTCTAGCCAAATGTTAACAACTCAGCTATTCTGGGGATGGGGGTGAGTCAGGAAACTGGCACCCAATCTTCCCCCTTCTCAACATTTGGGGAAAATAAAGGATTAGGTAAAACTCTTTTCGTTCCTCTCATTCTCCACCGTGAGCAGAGGTTCACAGAACCGGTGAAACCGTATTTAACTGGAGACAAGTCAATCCAGAAGGCATTTGCTCACACAGCAACCTTCCCACTAGAAAGTGAACCTTCCCGCTAGAAAAAAAAAGTGCTAACTGTTTTTATTTCATTCTTTCTTAGTTGGACCCCTCACCTGCTGCCTGCAGTTCTTCTAAAGAATGGCTGTGCAGAATACGACTTAAAGTTTGAGCAAAGTGGGGTGTCTCTAAATTCAAAGATTCCACAGAGAGGAGGTCCTAGCCCATAAAGAATAATCACAAGCTGGGAGTAGGGGGTGTGTGCCTTTCATCCTAATACCCAAGAGGCAGGCATAGGTGGATCTCTGTGATTTTGAGTCCAGTTGGGTCGATATAGTGAGTTCAAGGCCAGCCAGGGCTACATACTAAGAGACCATTGTCTCAAAAGATAGAAAAAGAAAAGAAAAGAAGAGAAGAGAAAAGGAAAGGAAGGGAAGGGAAGGAAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAGTCATAATTTTCTTGAACCAAAGAAGTGATCACATTTGCTAGTGATGGATTGGAAACCATGACGAGGTGAGAGTGGGTGGGATCTATCACCTAATGGAGTTATTTCCTTGTATGATGTGGTTTGTAAGAAGTGTCTGCCTCTTCCTGGAATTCAAGCCTTTTAGGAAAC
U3_D5 65374158 65374413 256 TCTGTGACAGACAACGGAGGAATGTGAAGGGAAGGTTAATACCCTTGTGCCTGACAGACTTTCCTTAGCAGAGCTGTGGAGTGTGACAATGGTGTTTGTGTCCAAACCATCAAACGCCATTATCACACTAAATAGCTACTGCTAGGCAATCCGTCCACTCCACGCGTGACTTGACGTCTGTTCTCTCCGAGCAAGAAGTTCTGTCTTGTGCTAGAGTTCTCCATTGTGTGTAATGTATGAATAAAATCTGGTCCTC
D3_G3 65374438 65377582 3145 CTGACGCCTTCCCCTCGTGTGCTATTGTGAAATGAATAATGCTATAAAGGAAAAGACCAGTAAAATCCAACCTCTCCTATGGGACCCTCCCAACACTGACTACCCTGACACCCAATTTATTTCCAGCACTAGGCCCACTCTCGATTTTGCTCTGAACGCTAGCGAGGCATTATTTCCATTCACTGTTGACACTGCCTCTGTGGAGTTAGCCCTGAATCCAGCAGGTGGATGGCACAGTCCCTAAGACCCAACTTCAGATGTCCATTGAATGTCCTAAGTTGCTTTCCTTGTGTTTCTGACTCACCAGCTATAAACTGGGGTTTCCACACCTGTGTTCTCAGGTCCAATTCTCTTGCTTGAGTGGGTCTAAAAATTCAGGAAAACGTGTTGGCCAGCTTATTCCAAAGGATAATTTAAAGGCAGAGTGGAGATATGTTCTCCGGGCACTGCTGCATATTTTTGGCTACCTAGAAGCTCTACAAAGCTGTCCTTCATAGATTTTATGGGTACCCCTTGGCATGGGTTTGATTCAGTAGACATGAAAATGTGCTGGCGACCCCACAAGCCTCATGGACAGATAAGGAGGTGATGTACAAAGTGAAGAATCAAACTTGAGTCAGCAGCATCTTGGCAGATATTGGTTCAAAAACTTCCAGAGTCTGATCTTAATGGTTGAATTTTCTTATATAGTTAAGCATAGCAAAACTTTGGGGGAAAGGACACCACATGACAATTATCACCACAACGCTTTAGGCATAGCTACATAGAGTCCCCCTAGCTGAGTAGCAAAGCACCTGTGACCTTTACAGTGAGAATGAATTCTATTGGCTGATAAGCAGAGCTCAAATCTAGAGCCCAATGAGAAAGGATTGGTTTAAGTGTAAGAATAGTCTCTAATGGTGGGGGATGCAAAGTTCTCATCACTGAGAGATAACGCTCAGCATCAGGACCTAAACAATGACCAGGCTATTGGGGGACTCGTGTGGAGGCTAACTCCTAGTGGAATAGCAAAGGGTCACCAGCAGGTTGCTTGACCATTTCCAGTCTGGCTTTCCAGACGTCATTCATTTCCCTCTGTTGGAAAAAAAAAAAAAAAAAACAGGCTTGCGGGACTGATCCAGTGTCTCCAATACTTCCTCTAAGCATGCTTCTTACAGAAATGTAGTGGGACAAAAGGGACAATCGAGGTCTAATGATTAGGGGGCGTGCAAGCAATGTTATCAATTCAGATGTTTCGTTCTTTGTAGTCTTAGGGGAGGAACCCCATAGAATGAGGAGAAATGATTATCAGACAAGGTAGATGGGAATTATGAGCCAGGAACCATGGATGAAAATATATGTGCATATACATACATACATACATACATACATACATACATACACATGTATACACACACATACACAAAATTAAATCATAATGATGGTAAATTTCCTTATTTTCCCTCATTCATTAAAATTAAAAAAAAAAAAGTCTCCCAGAGTACCCAGCACAAGGCTAGTCATCATTCCATTGAGTTGATGTTAGGAAAATGTAGATGCAAAAACAACTAAAACTAAGTGTCTTAGTTAGACTCCTATTGCTGTGATAAACACCATCACCAAAAGCAACATAGGAAGGAAAGGATCTATTTGGCTTATGTGTGCTGATTGCAGTTTATCTCGAGGGAAACCACAGTAGGAACTCAAGCAGGGCAGGAATCTGAAGGCAAGAGCTGTGATGAAGTCCATGGAAGAGTGCTGCCTGCTGGCTTGCCCCTCCTGGCTTGCTCAGCTTACTTTCTGATACACTCCAGAATCACCTACCCAGGGATGACACCCTACTCAGTGCTCTGAGCCCTTCCCATCAATCATCAACCAAGAAAACGACCCACAGGCTTGCCTATAGGCCAACCTGATAAAGGCATTTTCTCAGCTGAGGTTCTCTCCTCTCAGATGACTATAGCTTGTGTCAAGTTTACAAAGAAGCAAACACGTGAGGTTAGTCTAGTCTATGAAAACCACAGGCTGATAGGTGATAAATTAGGGCATAGATGTCCTCTTCTTTCTATCATTAAAAAAACAAAAACCTGTATGTGGTTTAATTGACCTTCCAGACATTCTAGGACATGTCTGATTTTTACTGTCTTGGCCAATTATCAGGTTGATTACTGGATTCATATGTTAGATCCATCTTATGTTTCACAAGTTAGGACTGGAAACCCACAGATAAACTGTATGTAAGTTGCCATAGCAAATTTGAATAATTTGAAGCCTTTCAAGTTCTGGGATAATGGTATGGATGAATGGTCTGGTTTCAAATACATTCTCTGAGAGGCATCTTCTGTAAAAACAAAAGTTCTGTTGTGGGCTTAAGTTCTCATGTGTGACTTACCTGAATGAACTAGTATACGTTTATACAAGTTAACAGGGTCAGATCAACCATCAGAAACAAATGGATCCATCCATCAGCCAGCACTTATCAAATGTGTACAGAGTGGCTTCCCTATGGCTTGTACAGTCCTAGGCCCTGGAGGGTACTTTTAAATAAAATGTGCCTGACATTATTCCTAGGAATGAGCAATCACTGGCTTGCATTCTACGGGTGCACAGTCCTCATGGCCCCAGTTGCACTTAATAAGGAGCCCAGTGAGAAAACTTTGCCAATGGATTATTCTCAGTTCCTTGCTAGAGAGCACCCTAGAAGTACATTCCACACATCTAAGATGACACAAAGTACATTTCTACCTATTCAGTGTGTTTCCCCCAACACCTTCTCTCCTGGCAAGTCAACTGTCAAGAGGAGATCAAATACCCCTGAAAGAATGATATCCTGGCATTCTTCCTTGTGTGTCTTTTTATGGAGCTAGCATTGCCTGAGCAAGTTCTGAGAGTGACCTTTGCCGTTATAGGAGTCGCTCCCATGCACATGGTTTCCTTGGACCTTTGCAACACAGGGCAAAGACAGCACAGGTTTGGGTGGGCCCCCTCCTGTAGACAGCAAACTGAGAATGGTGCTTCTGGTGTCATCTGCTCGTTCAAACACAATGCATGATGGATCTTACAAACGGAGAGTCCTGCTCTTCCTTTGTAGCCTTCCTTGATACGGTTCTTCCACAAAACCCTGTGCTTCACGGCTGAGTCCATGTTGAAACTGAATTAAACATAC
U5_15 65358996 65374045 15050 TCACTGGTCAGCCCTGAAAACATACTTATGTATTTTGGAATGAAAACATAAAAGGAAAAGGAGCCATGAATTTGGAAGAGAGCGAGTCAGGTGGGGGGTTGGGTGGGGGTCATATGTGGGAGGGTTTAGAGGGAGGAAAAGAAACAGGAATGATGTAATTAAATTATAATCTAAAGGAAATAATTTTTTAACGGTCTCACAGGCAATGAACAGCTTGTCCATTTTTCTTAGAACCATGTTTTTTTTTAAGTTAATAATGCCATAATGACAATCTTTTAAAATAACTAAAGATGTAGCAGTTATTCATCAATGAAGATGGTCCTTGTTATTTTGTGGGGTGGGTAGTAGCCAACTTCCTTCAAGATACGTTTGGTAAATAATGAGTTCAGTCAATGGAACCCAACCAAAATTGTTTGTAAGTATTAAGATAGGTATTTCTTGACATGCAAAGATGAAGGGGAAAACAGAAAGAGAATACAAAGTGTTAATATTTATCAAACTTTCACAAAAAATACTGCATTTTATCTGGAAAGCCTGTTTTAACATCTCTGAAGTTAGGATGCCTTTGGGATTTGGCAGCCTTTTTAATCATTGTTAACCTGGGATGGCAGACATATCATAACCATCAGTCATCGCTGCTCACGCTAATGCCCTCGATTGTGTTATCTGCATTTTGCTACAACACATTTTTGTCCCACGCTAACCATTTAAAGGCGCTTCGAAAAGTACACCAGCCTCAGTGTTGACAGGAAAGATTCTCTTGTGCAGAAAGACAGGAGGGCAAAGTAGGAGAGACTGAACTTGGCTTAGGGGTTCCAGTGTTCCTCTTGGAACAAACAATCACGGTTTTGTGTTTTCGTGGGAAGTGACCATCAAGCGTGTCACAGAACACAGGAAGGAAAACCCAAGCCGATGGTGGATGTTGTCTGTTATCATGATCTTATCATGGGATAGCAGGGCAACTGAAGCAGATCACTTTCTACCTGGGCCAGGTAAGTGGGGACAGGAAAGGCTGGATGGAGCTGAGCTAGAGAGGAGGAACCAGTCTGACTCTGAGGGCTGTGACTGCCATGTCCTCTGTGTCCCCTAACACAGTCCCATCCCGCAAGAGCGAAGGCCGGGATAGAGAGCAAAAGCTGTATGTGATACCAGGGAATGTTGTGCAGGGCACAGCAGCTCTGAGTGACAGGAGGAGAAACAGAGAAAGAGAAAGGTGTCAATAGGTTGGTATGGCACTGCCCCTTCAACAGCCCCTCATTAGTTCTGTTCTCTGGAACTAACCCTCGTCACCTAGGAGACTGTGGTAGATGGAAATGTCCATACACACCCTCTGCCTGGTCCTAAATCTCTGACCCACAAATCTTTCTTTCCCAGCAAAGTAATGAAAGGAAGGTGTAGCAACAGGAGTCCAAAATCACACTGCACAGTGGGCAGAAGCCAGGCAAAGGACCAGCTCGTGGGTGGGGTCTGTGATAAAACATTACATACCTTTCATCTACCAAGTGGACATCTTTCCTGCCAAGGCTGTAATGCCTGTCTGATAATGATAACGTTTATTGAGTGCTTGCCCTATATGTACTGCAGTAAAGTGTGTGTACATGCGTGTGTGCATCCTTAAAACTTGCTGTCATCTTAGAAGTTATTATTATCTGAACTTTAAAGAGTATAGATTACCCCTAATTCTATTCAGCAGGAAGCAACAAAACCAAGATTTAAAACCAGCTCCCAGTTCTCAATTTCTGCTTCAGGTATATTTTTCTGAAGAATCTCTGACTACCACCCATACCTTTCCGATCCGACTACTTTTTCTTTGTGTTTTTTGCTCCTTGTAACTCCCCCTTCCCTGCCCCTTGTCCCCTCCTCTGTACCACATAGGCCACCAGAGAGCTGTTAAATGTTCCTCCCATGATCCTCTGCATAACTCGTTTCCCTACACTGCTACAACCCCTACCATGATCTGTTTCTACCATTGTCTCCTGTGCTTTACAAAATCATGTTTCTCAAACCTGCCTCAGCTACTCCCCACAACCCCCTGAACACCTTACATCTGTTCCCCCACATCCCCTGTGCCTTTTACAACCTCCCGCACTCAGATCATGTCCCTAGCACTACCTTTGTCTATTGGGCCCCAGTGGCCAATTGAATTAAATCCTGGTACTCAGCACAGCCACCAAGGCACACTTGACCTATGCTAGAGAACCAGAGGGCCTGGCCTACACTCAGAATCAGCTGTGAGACTATTTTCTGTATAGGGCTGCCCTGCTTTGATGCTGAGCCTATCTCTCTAGTCACTGAGGTGTTTATGTGCCTACTGCTACACACAAGAGACGATCCTCTCTCCTCACTGTGACCCTTGTTTGAGAGCAGTGTCCACAGCACCTAAGTCTTTGTTCCCTTGCAAACCACTTTGCATTGAAATTATGTGTTAACCATACTGATCACACAGTCTGATCACGAACTCCAGGGCATGAAGGGGAGTCAGGTGGAACAAGAGTAGAGAAGACAGCACAATGCTCTCCCAAGAATCAAGGGAGGCAGTAAAACCACTGATCAAGGGCTTAGCGTGGTGTCCAGATTCATAAATTCTGGATGAATCTGAGTCTGTAAAACAGAAAATAAAAATCTCTGCCTTTTTTTTTTTTTTTATAAATGTGGAACAGTAAACATCTGTCACTGAGACAGAAAGCAAAGATGTTGGTTTGATGGTAAAAAGTCCTTGTAGCAGACACTTTTTAACTCCAGGTGAGTCTTAGGTTGCATCTACCAGTACCCTAAGGAGAGGAAACAAGGTTAGAGGCAGTTGTCGGAAGATGCCAATGGCTAGCGCTCAGAGGGACCTGAAGTATTAGGAGAATATGCCAGACAGTGTGTGTGGGGGGGAATAATAGGGGTGAGGACAAGATGAGAAGAAAGTGAGGGGCATGGGAGTGCCCGTCCTTAGTGGGGGAGCCCGTCCCTAGTGAGGGATGTCATCCTTAGTGGGGGAAGCCTGTCCTTAATCGGGGACCCAGTCCTTGGTGGGGGAGCCCGTCCTTAGTGACTTTCTACCACAGACCGCAGTACATTCTAGAAACACGCAGAATTTACACCTGGACTTCTCATGTCGACACCGGATCTAAAAGGAGACAGGAAACATTATCACAGTGACCATATGGTCGTAACTTCGTGCTGAGCCCCTATTTCAAGGACACACTGCAGAAATGCAAGCTGTTTGTTGCTTCATTAACTATTTCATACGCCTGGCTCTGGCCTTTGAAGAAATTATAGCAGAAACCACCAACCTGGTTTGACATCATTGGGTGTTTAATGTTTAACCATCTAGGTCACCTTGGAAGTCTCAAAATGCCCTTCTTAGGAGCATGTTAATCTCTTCTTAGGGCAGAGATAATTCTGTGATGCTAACCCAAGCCCCAGGAGGCCCTACATGAGGTGAAGTGTGAACAGATGAATCCTTTCTTCGTGTAGTAGTTCACAGATGATTTTGTTTGAGAAAGATCTGAAGATGATGAAGTGGGTTGGGGTGAATTAAGCCAAAACTTTCTAGTCACAAAGACTTGGAGGAAGGTTGGGCTCAGCCTCTGTCTCCTGAGAGTCTATAGTTTATGTATCAGTCTGTGAACATCTCCCTTCTCTCTAGCCTTATCTCCCTTGTCTTGCTTAGGGAATGAGATCTAAGGCTCTGCTGCTGTGGAGGTCTGTCTCCAGGGTCTTCTAGAATCCAGTAGAGGTCTCTTTTTGCCTCAGTATAATGGGATGGGGACAGGCCCAATATAACAACAGAACAGCTAGAGGAAGAACGTGGACTGCTTAGAATCAGGGCGACCAGAGTTTGAGAACGGAACTGGGTTTTAAGTTAGTCCTGGGAAAGCAAGAGTCATGGACTGTGGGTGACTGGCTTTCTTCACAAATGTATCAGGGAGGGGCTGGGAGGCAATAAAAATTGCCTTCAGAAAAGTACACTTCAGGCCCCACTGACGGGCCCCAGCTGTTACTTTAAGCTACTAAAATTAAAAAAAAAAAAAAAAAAAACAAAAACAAAACAAAAACAAAACAACTATCAAATTGCTGTGAAGATATGGTAACAAACCTTTGCAGTACTGCTTTGTGCAAGATGCTCCTAAGCTTGGAGTACACTCATTCATTCATGAGTGCCTCAGAGTGACCTTGGGAAATATGACTACCCCAGATAAGAGATTTATCAATGAGGGAACTGAGGGCTAGTGAGTGGAATGGCTGGGTCTATGTCCAGGGAGTCTAGCTCCAAGATCAATGCTGCTACTGCTAGACTGTAGACCAGAACTCAGATACAAAGTAGCAAAGAGTACAAAGTACTCAGATACAAAGTAGCAAAGAGAGGAGAGCCATTAAAATGATTGTGTTTCCACTGGGCCCCCAATGGAGGAGCTAGAGAAAGCACCCAAGGAGCTGAAGGGGGCTGCAACCCTATAGGTGGAACAACAATATGAACTAACCAGGACCCCCAGAGCTTGTATCTCTAGCTGCATATGTAGCAGAAGTTGGCCTAGTCGACCATCATTGGGAAGAGAGGCCCCTTGGCATTGCAAACTTTATATGCCCCAGTACAGGGGAACGCCGGGGTCAAGAAGTAGGAGTGGGTGGGTAGGGGAGCAGGTAAGGGGGGAGGGTATGGGGGACTTTCGGGATAGCATTTGAAATGTAAAGGAAGAAAATATCTAATAAAAATTTTTGAAAAAAAAAGATGATTGTGTTTCCCTGATTTTAAAATACAGTTTCACACACACACCTGAAACCTGTGTGTATCCTTCTCAAAAACTGTAGACATTTGGCCTGGTTCTTCCCCACAGTCCCCTGCTCTCAGAAATGAGACTCAGACTCAAAATATATTTACAAATACTTTGGCCATATCTAGGCTCTTCTCTGCCTGGATCATAACTTAGATAATCCAATGATTTTAACCTACATTTTTTTCAATATGGTTGGTTACCTGTGCTCACATACCATGTGTCTGTCTGTCTGTCTCCTCACACCTTCCCTGGGCAGATCTTCCCATGCTCTATCCCAGAATTTTTTCTCCTCCCGGATGTCCCACCTACTATTTCCTGCATAAGCTATAAGCCACAGGCTTTTTAATCAACAGGTAATGAATGCATCCATACAATACACAAGATATTCTTCCTACCAAAAAATTCTTAGGCTCCTTTAACTATGAAATGGGCTTTTTAACTATTTTGAGACAAATCCCAGCGTGGAGGGCCATCGGCCAGGAATGTCTGGATCCTCTAGTGTGCTGTGGTTTACTTGGCGACTCTGTGCTCGCCCAAATGGATGTGCACCTGTTCGTGTGCAAGCATGTACCTGTGTCGAGGTGTGTCAGAAGACAACCTCAGGTGCCAGTCTGTCTTTCCTCTAGCACTAGCCACTGTTCTGACAATCTTTGTACCCCTTCTTCCCCAGTGTTCCCCAAGTCTTAGGTACAGGGGTTGTATTGTTTTCTCAATCGGGGCTGATCACCCCAACATCTGTTGTTCTCTGCATTCTCACTAGCTGTGGCTTTCTGTTTGGGGTTTTTGCTATTGTCTTGTAGGGTTTCTGTTTTTGTGATGTAGGACTCATCAAGGAGGCTAGGCATGCTGGCCAGAAAGCCCCCATGCATCTTTGTTTCTGGCTTACCAGTGCTGAGACTTCAAGAATACTCCTAAATCTAACCAGACCTCCTCTTGCTGGTAAGAACATCGCACTACTGACTGAGCTGTCTCCCCAGCTTTCCTGCTATACTGGTTCTGTTCTCTGTCCCCTACAGTGGCACACACTACCATAACCTGTGACTTTCTCCTCACACCCATCTGAGCCATTCTTTAATGTATATGTCATTTGTCTACTGGGCTGATGCTCCTTGAAGAAGGCTTTGTATAACTTGTATAACAACAGCACCAGTTGACATGGCAACATAGATGGGGAAATCTCACAAGGCTCTACCCCTAGATGAAGACCCACAGGCAGTTAACTTTAGGGCTGCTGAGAGAGGGGGAAGCGGTTTACTGAAATAGTCAGCTTTAAATACACATATGAACAACAACACTAAGAGGGTTCAGCAGGCTGTATTTGCATATATATGTATGTGCGCATGTTTTATGTATGTATGCATGTATGTGCATATATATAATAAGAAAGAGGTCATGAATTTGAAGGGGAGTCAGGGGTGTAGAACAAGTTGGAGGGGAAGAGATAGAGGCAGAAATAATGTAAACACAGTATCTGTCTATGAAATTCTCAAAAAGGTTAAAACATTTACTTTTTTTTTTTAAAGCCAAACCTTTGGTTTTTATTTTTTAAGAAATAAACAAGATGGTTTCTTTTGAGAGATGGTAGCTCCAAGCCATTCAGGGCTGCAGTTCAGCTCAGATGTCTAAATCCCTGATGTTTGAGGTTGCAGAAAAGAGAAGGAAAAAGAAAAGAAGTAACTAGCTCCAGTGAGGGATGCCCAGCTTTTGCCTGGAGGGACAGTGAAGTTTGGGAAGTGGGAAGCCTGGTTTTCACCGTTTGAATGTTTGACTTTGAACTTTTCTCTGTGTAGAAATGCTGAGGATACTAGCTTTTTATTTGCTGTGAATCCTCAGGTAACACAGAAGATACACTGGTCTTTAGAGCAGAGAAAACCCAGCGTGGATGTAAAGACATTACAGAGGTCAGGAGCTATACCTTCTCCTGGGTTGTTCATTCTCTAGTAAAGAGAGCGCTGTCTAGCTTAGCACACTCAGGGAGAAGGCTAAGGCAGAGGCTAAGGAAATGCTGACTTGTCAAGGCCTCTGGTTGATGACTAAGGCCACTCACTTTCTAACTGATGTCACAATCTAGGACCTGGGTAAAATCTATATTGACAGCAAGCTCTGGTTCATCACTGCCCCTCACAACTCACTCACAGTCCTCCTGCTATTAAAGGAAACGGGCCTAAGTCCCTGGTGCAGCCTGGGTTGGGGGTTGGGCAAGACCTGGCTATGAGGGCCAAATACTGACAGGGGTGGGGGTGGGGGTGGAGGTGAAGGTGAAGGGTGGTCATTGCTTGAGCGATCAGTGCCGGTTCTCAAAAGACAGTTAAAAATAAAGCTTAGATATGATGACTTTCCCCTCACCCCAGTAATCACTTTAACATACATCCCCAGAGAAAAAGTCCACCTGTCCTGAGTCCCATCATCAGAGCTAGTTAATAATTCTCTAATAATGTCTTACAGTTAGCTCATTCTTAAACTTCTCAATTGTCTCTGAATGCCTTTTATAATTATTATTTAAAACAACAACAAGCTAGCATCTAAGCTGGGTATAACAGCACATACCATTGCTTCCAGCACTTAGGAGGCAGAGGCAGGCAGATCTCTGTGAGTTCAAGGCCAGCCTGGTCCATAGCCATGGTCTACCAGGGCTATACAGTAAGACGTCATTTTGGAAAAGCCAAAACAAACAGACCAACAAACCCAAATAACAACCACCACTACTAGCATCTAATCGGAATTTGTATTTTCATTTAGACTTTCTTTCCTCTTCTTTTCAAATGACATTCATCCAATACTTCTCAGCATCCTCATCACATGTCACGTTTCCGGTATTCACCACCACCAAAGATCAGCTACTTCTCCCAGTTTCCTAAGAAGCAGATGTTCAGGATACAAACCTTTCACACACACACACACACACACACATACACACACACACACACACACACACACACACACACACACACACCTCTTACCCCGTCTTTCTCCAAGACAAATATAATTCTGAGATTTTTATGGGTGTGGCTTCCTGTAGACACAGGAAGCAAGGTTGTCCTCTGGATCTAGCAATCTTTCCACCCTTCTTCCTCAATATTACCCGAGCCTTAGGTATAGGGGTGATACTGTAGATGGATTGGTTGGGGCTGAGCATGCCACCATCACTAGTTCTGTGTAAGTTGACAAGCAGTGGCTTTCTATAATGGTCTCAGTTTACTGCAAACAGAACCTGGAGGAGGGCTGAGGCTACACTTGTCTGTGGATATAAGCATGAGTGTTTACAGTATAGTGATAAAGCACATATAGTTAGGAATGACACTGGTTTAGGAAACTGACCACAGTAGCTCCTCTTCTAGGCACTGTGAGCTCAGCAGACACAGGTAGTTGGGTTATGTTTTCAGTACAAGGCAGGACTTATTTCACGGTGAGTGGCCTCAAGTCCACTGACAGAGCTATTGCTTGTACTTTAGATATACATGCCACCATCACACCCTTGGCCATTGGATTTGTCCATAGGCTTTAGAGCTGAGTAGAACTATTGATCACCTTTCTCCATGGAGGTTCAGACCCAGAAAGACAAACACCACGTGGTTTTTCTTATTTGTGAACCTTAGCACCAGTATTTTAGATGTGAGTATGTGACCTGGAATAATGACAGAAACCAGGAAAGGAAAACAAGGTCCACGGTAGAGAGAGGAGAAGGAGCTGGGGGGGGAGTGGAGGGAGTTAGGACACAGTTGCTATGAAGGGAGAAATGGGAAAGGGGATGTTTAACTCCGATGTGGGTTGGGAGGGCGATACAGATGAAGAGGGGAGAAAGGAGTAAATAACACTAAGAATGTTTGAAGAAGCTATAAAGAAACATTCGATGCTCACTTAAAATACATATAAAATGTAAATAAGTATATTTTATGCATATGCATATTTAAATCAAGTTACACCACTTGAGTGTTAATGCCTTCCCACCCCCCCTACCCCATCGCTGCCAAGAGCTATAGACTTGCTAATAAACACTCCAGTGCCAGGCATGGGTAACATCCCTTCCAGCTGTTGGTTTGGGGAATCTAAGAGGCTTCTGAAATAATTCAGGTTATTACTGTTGCCCTTGGTGGCCTCCAGAGCCTGAAAGTGAGATCCTGTGGCTGAAGACACCACACACTTCAGACACAGGGCTGGGATGAGTCTAGCTGGTGCTGGCCAAAAGCCTGCAAGCCCTGAGGACAAGCTCTCACGGGTAAAAGTCAGTCTTGAAGGATGCTCAACGAAATTTGGAAAAGACGCATCTTCTCTCCGGGGTTTGAAGAAAATCGTGTGAAAGCTGCATTTGCTTCATCGTGCTTTCATAGACAGAGGGGTTTTGTCCTTGATATTTATTTACCGTCTTCTCACCGAGGTGGAGCGTTGACCCCAGGCAGTGCTTTGCCCACACTAGGCAAGCAGTTCATCACTGCGTTACATGTCCCACCCCACACCCCAGCTGTCTGACTGAAGGAACATGGACGAGAGAAGGTACTGGAAGTGGCCCGGTGGAGGGAGCGATGTCTTATCCTTGGAAAGATGTCCACTGCTGAACTCTGAGGCGTTCATTTCACTTTAAAAGAAGAAAACCCGCAGTTCTTTTGTAAAGAAATATGGTTACTGTGAGCTGGAGAAGCAAGACCGGTCAAAGCAAAAGCCACCACGGGCAGAAAAGCCACCACAGGCGGGAGGTGGCCCATGAAGCTACTCCCTCAATGCAGCCTTTAGAAATTTCCTGATCGCCATATCTGCCATATCTGCGTCAGGAACCTGGACCTTAATGGTTTCCAGAGGGCCTTTAAGGTAGTTTTTCCTCCGGGTGGACCTTGGGCTTTTACCTTATGAACGTGTCTCCCAGTCCCTCTGAAGACACAGAAAGAGACAGTTTTTCCAAGGGACAAAGAACGCAGGATTTACCTCAGGTAGTCAAAGGACACTAGGGAAAGACTGAGTCTCCCAAGGGAAGGAAGCAGGGGACAGCAGTGGAACCCATGAACGGCCACGGTTCTTTGTTCTAAGAGACCAGTTAACCACAACACTGTCAGCCTTCGGCTTTTACCTAAAAGAAGAAAGTGTGAGGCTGTGTGACAAGCCCCACAACTCAAGGCTGCTGGGTCACGGTCTTTCTCAAGAAAGTCCTGATTTCACAATAGATTGCCAAGGAGAGTATAAAAATGGTTTCTTGTTTTGTTTTGGTTTTTTAATGCTTAAGAAAGCAATCTGTCAAAACATCCTAGAAAGGGGGAGAGGGCAACCCAGTGCATTTAGGACCCTGCAGACGAGAGTGGCTATTGCTGCTTGTGCGTCTGTTCTGACTCCCAGGTAACAGACTCTGCTTGTTCCCCCTCCCCTTGGCTTCCCGGAGCTCAGCTGTCAGGGAAGTGATCCATACATGGCCACCGGTGAACAAGTGTGACTAGATAAGATTCCAGCTCATCACAAACGACCAATATCAGTCAAGAGTTTTGAGTAGGATGAGTTGGGAGGCTTAATTTTGCTGGTTCATATGACCAGGTGACTTCTCCAATTCTAGAAGTCTGAAGTGGTGACACAGGGGGGTAGGGTGGGGGTGGGGGGAGGTCTCCCTGTGAGGAAAACACTGGAACAAGTTTAGATCAGAGTCCTGAGAGAAAATGTCCAGTAGGTTCAGCTGCCCACTTTAGGCCTTGGCCTCCCAGTCCACCTGCAGACGGCGTATACCACGTAGCAGAGGGGGCATGGGGGGAGCTGGACCTTCGGTTTTGCTTAGTGTTTGACCAAAGGTGACTCTGACTTAGTGGACTAAGGTCATGCCCTCTCTTCCCCCACAATCGATAATTTAATGTGACTTATCAGAAAGAGAACGAATTGTTTACTTTTAAACCCTGGATCCCATAAAGGGAGAGTGCACAAAGGCCTAAAAGCCACAGATGCTCGGTGCTCTCACGCACGCAAGGCTGCCCTCAAACCCTCAGTAGAGAGAAGATAAACACTGTCGGAGGTAAGTGCCTTCTTCCAGGGCCTACAGTGGGAGTGAGGCTGCCTGCCGAGGGGAGAGTGAGTTGCGGGCTCAGGTGTTTAAAAGCCGCCACACAGCTCCTGAGCAACACTGGGCATAAAACATTCCCTAATTGAATTTGGTCTGCCATGATTATTCATTCTGTTCAGAAAGCCTGAGCAAAACTGGACACTTTCTGAAAGCTTCTTTATACTCCCTAGAGAGGGGAAGGGGAAGGGGAAGGCTAGAGACATATGGAAGACGCAGAGGCTGGCTTGTATTTCTATCCTTGGAGTGGCTATGGTAAGCCATGTCTCATTTAAGAATGGCCTGGTTATCAATAGCATCAGGGCCTTGGATGAAGATTCTACCTGTCTGTTTAAAATGGGGGTTTGTTTGACAGGGGCTCCTCTTTGTGTGTGCTTCACCATGCCTTATTCTTATTTCAAAGACACCTGATCCCAATTCTTCCTCGTGTGTGTTCCAACTTGTACTGTCCCCTCAATGATGCTAGAAATAGCTGTCCACCTGCATAGAGGTGAAACTGGTCCCTAGGAGTCCCGAGCTTCGGCAGCCCTTGATTGAGAGTCCATCGAGCCTTTAATGCTGAGAACCTGGCGGAGGATTCTCTCTTTGCCTCTGGTTGGTGACTAAATGGCCGATTTGATTGATGTATGGATAAGGAACTTTTCCAGATTTCTCCCTTTCTAAATGGGTTTCCTTGAAAAGAAAAGTGACTCTGTTCCCTGGTGAAAACAAGAACATTCTATTTTAAAAGTTCTGTGTGCTTTCAAAGATGACAAGACTCGGGAGATATGAACTGACTTTCTTTGTCTAGACTGTACTAGCCTAAAACAGCCAAATGGATACATTTCACACAACAACTTCACATGACAGTAAATGCAGGTTTGCAATTTTTTTTTAAAGCATCTCTGCTTTTTGCTTTTTAAGAAATCCTTTTGTGGTATTGATGGGTTATTTTTTGCAGCAAAGTGACTCCACATAGTTCCCGAAGCTCCCCCTTCTTGGTCCTGGGAAGCTGTTTGCAGGATGTTCTTCGCTCCTTTGAACACGCTGAAAATACAGATTCGTGATCACCTGGTACTTGTGAATCCACCACATCTTCCTAGGCGCTGTGGCGCGCGCTGATGCATCTGAGGCAGGTTACGCTTTGCCAAGGCCTCCTCCTTAGAGACCGCCCACTCCCACTTAGGAAGAGAGATAGATGCCTGGCATTAGACAGGGAGCAAACGGAGTTTAACTTGTTTTTCATGCACGGGGGCTCAATGGGGAAACTCCATTTGGAATTGTCCACAGCCCCTAGAAATGTCTAGAAATCCCATCCTCTTGGAGAAGGGGAGGACAGGACACAGAAATCTTGTTTTAATAGGCAGACACTAAGGGACCCTTGTCACTCAGCCTCCCTTAGGGAGGACTTAGGAAATAAGGCACTCTGACTGCTAAATGGTAGGAGGTGTGTGGGTCTGAGCTCAAGAGCATGAACCCCTTCCTGCGCTACGGAAACCCTGCTGGCATTTGGACACATCACCCTGATTAACATCCAACACCATTGGTCTTAGTCATTGCTTTGTTGATGTTGTTTGTTTTTTTTGGGAGGGTGGGGGTCGGGGGATGTTCATAGAAAATAACAGATGCTACCATTTTTCCATCCAGCATGTGCACTTTCTGTTGGATATTAGCTAGTACAAGTGAGCTTCCCAGTGTTCAGTCTGAAAATTCTAAAGCCCTTTTAAGTCTGAAGAAAGGAAACAGACAATGTGGCTTGGACCCTAGAACGAATGGCTTTGGCTTTTCTTCTCGGTGATACATTCATTAGTAAACAACATTTGGAAGAACAGGAAAATATTCTGCTGACAGAATTTGATCACTTGGTCAAAGAAAACACAGAAAAACAAAACAAAACAAATGTTTCAGTAGTGTCTAAAAATTGTTCAAAGGGCCCTAATAAATGCGAATTTATTTCTTGATATAATAGCATAAAGTTAACTATGCTGCATAGTACTTCAGTAATTATTTGCTTAAAAAAAATTTTTTTTGACCTGAAGTCTCTCTCTGTCAGCAAGACTGTTTCATGGTGGCAAATACATCTGTTTGAGTCAGATAGGTTCCAGCTTTCATATTTACGAAAAGTGTTTACAAGGAGGATTTATTAAATGGGGTTATCCAGAAGGAGGAGGGAGGACTTTGAAATTTTTAAAATTCTCATTATTTTTCTCATTTCCAAAGCACTGTGAAAAAAATTGTGAAAATTAATAAATTCAATGAAACAGAGGTAAAAGTCCAACAGCAGCTCCCTTTCCACCTGGTCGAGCGCTACAGTAACATGAACGCTGTACAGTTCTCCCTGAAAAGGGTCCGAATCATATGCACTGTTTTGAAAACCACTCCTCTGTTGGAAATGAAATCTTACTTTGTTTCCAAGTCAACAACAGCCAACTTTTTACCGAGTCGATGATTGCAAGGTGAGGACCGTGGTCCAGGTGAGTGTCGACCATTTCCCAAAGCATGAGCCATGTGCAACCCTTTATTTTAAATAATGTCACTATGAATTAGCTCTTATTTACTCATTTAGTTAGTTAGTTAGTTAGTTTACCTTTTCCTTGAGATGTGTGCTTGATTCATGACCTGCCCGAAAGGCAGAGAGGTTAGGGGAGGGTTGCGTGTAATATGTTGGTTGGTTCCCTCCTGTTCTTCCTCCTCTGTCTTTTGCACATCAAGCACTGAGAGGTCCTCAGCCCCTACAGCACTAATGAATGCCTCACCGCTCCTGGTCGGCCTTACTACACGTGGGAAGGGGGTTCTAGAGACCTGCTGAGATGGTCAGAGCATCCCCGGGAGGGGGTGAGGGATGAAAAAGAGGTGGGATTTCAGTCCTAGGTCCTCCAGGCAGAGAAATAAATCAGCATAGCTGGTCTCTTGGAAGGAGTGGTGGGGAAAGACAGGTCATCACCTACACACACACACACACACACACACACACACACACACACACACACACACACACACTCTCGAGGTCCCCCATCATTGCATGGCCAGCACAAAGGTTTTAGGGTGGCGCTGGGTGTCAGCAGGAGCTTCCTGGTTTCACAGAGGAGTGCTTGTCTAACACTAGGCTGAGTGACACTCTTTTCCTGCAGTCTGCTGGGGCCACAGAGGAACAAAGGAAGCCAACTATTATATTTTATTTTTTAATTTTTTTTAGTGGACAACAATCCTGTCAGCACCATAGAAAAGGAAAGGGGCGAAGAGGAGCCAAAGTGGGAGGGAGGTCGGAGGTCACAGAATGCTGTTCCTGCGGCATCTGTCTTAAAAGGGTGTCTAAAAGGTGTCTTAAGGTGGTCTCTATGGGAATACAGGATAGAAGAATGCGGGAGGACTCGTTTCGCTTGTTACAGTTTAAGCAAATCTCTAGCCAAATGTTAACAACTCAGCTATTCTGGGGATGGGGGTGAGTCAGGAAACTGGCACCCAATCTTCCCCCTTCTCAACATTTGGGGAAAATAAAGGATTAGGTAAAACTCTTTTCGTTCCTCTCATTCTCCACCGTGAGCAGAGGTTCACAGAACCGGTGAAACCGTATTTAACTGGAGACAAGTCAATCCAGAAGGCATTTGCTCACACAGCAACCTTCCCACTAGAAAGTGAACCTTCCCGCTAGAAAAAAAAAGTGCTAACTGTTTTTATTTCATTCTTTCTTAGTTGGACCCCTCACCTGCTGCCTGCAGTTCTTCTAAAGAATGGCTGTGCAGAATACGACTTAAAGTTTGAGCAAAGTGGGGTGTCTCTAAATTCAAAGATTCCACAGAGAGGAGGTCCTAGCCCATAAAGAATAATCACAAGCTGGGAGTAGGGGGTGTGTGCCTTTCATCCTAATACCCAAGAGGCAGGCATAGGTGGATCTCTGTGATTTTGAGTCCAGTTGGGTCGATATAGTGAGTTCAAGGCCAGCCAGGGCTACATACTAAGAGACCATTGTCTCAAAAGATAGAAAAAGAAAAGAAAAGAAGAGAAGAGAAAAGGAAAGGAAGGGAAGGGAAGGAAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAGTCATAATTTTCTTGAACCAAAGAAGTGATCACATTTGCTAGTGATGGATTGGAAACCATGACGAGGTGAGAGTGGGTGGGATCTATCACCTAATGGAGTTATTTCCTTGTATGATGTGGTTTGTAAGAAGTGTCTGCCTCTTCCTGGAATTCAAGCCTTTTAGGAAAC
D3_15 65374438 65389487 15050 CTGACGCCTTCCCCTCGTGTGCTATTGTGAAATGAATAATGCTATAAAGGAAAAGACCAGTAAAATCCAACCTCTCCTATGGGACCCTCCCAACACTGACTACCCTGACACCCAATTTATTTCCAGCACTAGGCCCACTCTCGATTTTGCTCTGAACGCTAGCGAGGCATTATTTCCATTCACTGTTGACACTGCCTCTGTGGAGTTAGCCCTGAATCCAGCAGGTGGATGGCACAGTCCCTAAGACCCAACTTCAGATGTCCATTGAATGTCCTAAGTTGCTTTCCTTGTGTTTCTGACTCACCAGCTATAAACTGGGGTTTCCACACCTGTGTTCTCAGGTCCAATTCTCTTGCTTGAGTGGGTCTAAAAATTCAGGAAAACGTGTTGGCCAGCTTATTCCAAAGGATAATTTAAAGGCAGAGTGGAGATATGTTCTCCGGGCACTGCTGCATATTTTTGGCTACCTAGAAGCTCTACAAAGCTGTCCTTCATAGATTTTATGGGTACCCCTTGGCATGGGTTTGATTCAGTAGACATGAAAATGTGCTGGCGACCCCACAAGCCTCATGGACAGATAAGGAGGTGATGTACAAAGTGAAGAATCAAACTTGAGTCAGCAGCATCTTGGCAGATATTGGTTCAAAAACTTCCAGAGTCTGATCTTAATGGTTGAATTTTCTTATATAGTTAAGCATAGCAAAACTTTGGGGGAAAGGACACCACATGACAATTATCACCACAACGCTTTAGGCATAGCTACATAGAGTCCCCCTAGCTGAGTAGCAAAGCACCTGTGACCTTTACAGTGAGAATGAATTCTATTGGCTGATAAGCAGAGCTCAAATCTAGAGCCCAATGAGAAAGGATTGGTTTAAGTGTAAGAATAGTCTCTAATGGTGGGGGATGCAAAGTTCTCATCACTGAGAGATAACGCTCAGCATCAGGACCTAAACAATGACCAGGCTATTGGGGGACTCGTGTGGAGGCTAACTCCTAGTGGAATAGCAAAGGGTCACCAGCAGGTTGCTTGACCATTTCCAGTCTGGCTTTCCAGACGTCATTCATTTCCCTCTGTTGGAAAAAAAAAAAAAAAAAACAGGCTTGCGGGACTGATCCAGTGTCTCCAATACTTCCTCTAAGCATGCTTCTTACAGAAATGTAGTGGGACAAAAGGGACAATCGAGGTCTAATGATTAGGGGGCGTGCAAGCAATGTTATCAATTCAGATGTTTCGTTCTTTGTAGTCTTAGGGGAGGAACCCCATAGAATGAGGAGAAATGATTATCAGACAAGGTAGATGGGAATTATGAGCCAGGAACCATGGATGAAAATATATGTGCATATACATACATACATACATACATACATACATACATACACATGTATACACACACATACACAAAATTAAATCATAATGATGGTAAATTTCCTTATTTTCCCTCATTCATTAAAATTAAAAAAAAAAAAGTCTCCCAGAGTACCCAGCACAAGGCTAGTCATCATTCCATTGAGTTGATGTTAGGAAAATGTAGATGCAAAAACAACTAAAACTAAGTGTCTTAGTTAGACTCCTATTGCTGTGATAAACACCATCACCAAAAGCAACATAGGAAGGAAAGGATCTATTTGGCTTATGTGTGCTGATTGCAGTTTATCTCGAGGGAAACCACAGTAGGAACTCAAGCAGGGCAGGAATCTGAAGGCAAGAGCTGTGATGAAGTCCATGGAAGAGTGCTGCCTGCTGGCTTGCCCCTCCTGGCTTGCTCAGCTTACTTTCTGATACACTCCAGAATCACCTACCCAGGGATGACACCCTACTCAGTGCTCTGAGCCCTTCCCATCAATCATCAACCAAGAAAACGACCCACAGGCTTGCCTATAGGCCAACCTGATAAAGGCATTTTCTCAGCTGAGGTTCTCTCCTCTCAGATGACTATAGCTTGTGTCAAGTTTACAAAGAAGCAAACACGTGAGGTTAGTCTAGTCTATGAAAACCACAGGCTGATAGGTGATAAATTAGGGCATAGATGTCCTCTTCTTTCTATCATTAAAAAAACAAAAACCTGTATGTGGTTTAATTGACCTTCCAGACATTCTAGGACATGTCTGATTTTTACTGTCTTGGCCAATTATCAGGTTGATTACTGGATTCATATGTTAGATCCATCTTATGTTTCACAAGTTAGGACTGGAAACCCACAGATAAACTGTATGTAAGTTGCCATAGCAAATTTGAATAATTTGAAGCCTTTCAAGTTCTGGGATAATGGTATGGATGAATGGTCTGGTTTCAAATACATTCTCTGAGAGGCATCTTCTGTAAAAACAAAAGTTCTGTTGTGGGCTTAAGTTCTCATGTGTGACTTACCTGAATGAACTAGTATACGTTTATACAAGTTAACAGGGTCAGATCAACCATCAGAAACAAATGGATCCATCCATCAGCCAGCACTTATCAAATGTGTACAGAGTGGCTTCCCTATGGCTTGTACAGTCCTAGGCCCTGGAGGGTACTTTTAAATAAAATGTGCCTGACATTATTCCTAGGAATGAGCAATCACTGGCTTGCATTCTACGGGTGCACAGTCCTCATGGCCCCAGTTGCACTTAATAAGGAGCCCAGTGAGAAAACTTTGCCAATGGATTATTCTCAGTTCCTTGCTAGAGAGCACCCTAGAAGTACATTCCACACATCTAAGATGACACAAAGTACATTTCTACCTATTCAGTGTGTTTCCCCCAACACCTTCTCTCCTGGCAAGTCAACTGTCAAGAGGAGATCAAATACCCCTGAAAGAATGATATCCTGGCATTCTTCCTTGTGTGTCTTTTTATGGAGCTAGCATTGCCTGAGCAAGTTCTGAGAGTGACCTTTGCCGTTATAGGAGTCGCTCCCATGCACATGGTTTCCTTGGACCTTTGCAACACAGGGCAAAGACAGCACAGGTTTGGGTGGGCCCCCTCCTGTAGACAGCAAACTGAGAATGGTGCTTCTGGTGTCATCTGCTCGTTCAAACACAATGCATGATGGATCTTACAAACGGAGAGTCCTGCTCTTCCTTTGTAGCCTTCCTTGATACGGTTCTTCCACAAAACCCTGTGCTTCACGGCTGAGTCCATGTTGAAACTGAATTAAACATACTCCTGGTTGATTAGCATCCTCAGCCCCTCTGTAGGTCTGCCTCTGGTAAACGCTGAAGGAAAACCCAACAGAAGGATGGGTGTGCAACAGTCCCCATGAAGGTTCACAGTGCAGAGAAAGCCGGATGTTCCAGAACATTCTCATTGGAAAAGACGAAAGAAAAGACATGTTTGTGGCAGTTAACGTGCCCAGACATGGAGCAGCCATCCTGGAGACCTCCTCAACTGTGATGGGTAGCACTGAAAGACACAAAGTCTTCTAGCTCAAATTATTCTTAGATTTGAGGGCAATGTTCGTTCTGTTCCACCATGCTGGGACCTTAATTAGATGGAATTAACCCTGGAACATGAAGAAGGGCTTTCTGTGTTCTTTTCTACTACTGTGGCTCATTAAATGGCGTATTATGATTGCTGCCTACATCATTCCTCATTCAAAATAAAAGAACTGTTCTTTCTGTAGGTTTTCCAAGGGACACATAAGGAAGGCGAAACATCGATGTGCCCATTTTGTGTCACCATGTGTGTATGCCCATGTGCGTGTGTGCACACATGTGTGCCTGCATTCGGTGTGTGTGTGCCTTCCTTCTCTCTCTATGTGTGTGTGTATATTCATGCATGTGTGTGTTTGGGGTGTGTGCCTTCTCTCTCTCTCTCTCTCTCTCTCTTTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGCGCGTGTGTGTGTGTGTGTGTGCGTGTGTGCATGCTCCTTGGTTTTTCTCCACCTTGTTTTTTATGAGACAGGGTCTCTCATACCTGGAATTTGGCTAGCCTGGATGGCCAGCAAGTTCCATAATCCAAAATGGGATTGTAGGCATGCACTACTGTGTCCACCTTTTTAAATGTAGGTTCTGGAGAATCAAAGTCAGGACCTTATGTTTGTGAAGCAAGCTCTATACTGGCTGAGCCATCTCCTCAGCCCTTACATCTCCCACTTTAAAAACACTGTTTATACAGCCCTGCTGTTTCAGTTCTCTGACGATAATATACTTCCCCACATCAGCTTTCATCTCCTCCCCTGTCCCAACACAGATATGGCCAGCAGTTTCCAGGCAACCATTCCTAGGGATGCATACTGACTGGCTCCAAATGCTGTTTGCAAGATGAAAGGGTCCACTTGGACCTGTTATTTAGAGTGACTCTCAACCTGGGGAGGCAACAGAGGATGCAGATGGCCACATTAACTAGTTTTTCTTAGCAAACAGACTACCTGAGTCTTACTTGACTTTGTGGCTTCCACTAGTGAGGTCTTTAGTCCATCTTTCCTTCTCTGCATAGTGTGTTCAGCAAACATGTCAATCACCACTCAGGGCCAGTCCTGCTATCGTTCAGCTCCAAGGGAGTCACCATATGCCAATGGCTCATGCAGACGCTCCTCTGGACCCTTCTAAAACATGTAAATCTTACATTTGCATCCTTCTTAGAGGAGATAAAGGGAGGCTAGGATTAGAAACCAGCTCACTCTTTGTTCTGATGGGAAGTTGAAGCCAAGGATTCTAAAATTTGCTAGTTGATTTAAAAACACCCCCACTCCTTAGCATCATCACTCACTCCTGGGGGCGGTGGGTGGGGGAGGTGACATGGCTTGTATAGTTACTATGCAGTAGTTATTGTACTGGACTCTGGGGATACAAAGAATCAGACTGAGTCCTCTTCCTAACCTTTCCCATTTAGTAGTTGGGTGGGTGCCTAAACACATCCATTCCTCACTGTGATAGGTGCCAACCCAGAGGCATAAACAGGGAGCCAAGAGCTGTGAATTATATCCTTTATTTCTCACCAGAGAAAGAGATCTCAAGTCTTTGATGAGAAAGAAGCTCTTTCTTTGTTGGGGTTGGGAAAGCTTCCTCCCTGTTCATTTTAAAGATGCAGAAGCTAACGGGCCGTTGTCTGGCGCTCTGATGTCACATCATCTGAACCTGCTGCTTTTACTACTCTGTGATTTGCCAGCTTAATGAACAGCTCAGGGGTTGGAAGATGAAGCTGTATGTTTTTCAGTGACACGAGAAGAATTTTAATTTCCCAGAAGAGCTTGGCAGGATGTAAGCTGCCTGGGTTGACAATGCTCTTACAGAACCAGGTCACAGGTTTCTCTCCTTCCCGACTGCCTCTTTCCAGCTACCAAAAACCTACAGCTAAGTCCCTAGATGAACACCTCACTACCTGCCCTCCAGACAGCCATTAGAGCAAACCCACAGTAGACACCATCAGTAGACAGGTGGGGACATCTGCTTTCTTCTCTCTGCTATCGAATTTACTTCTCTGCTTTATGCTTGAAAAGGAAAGGAGCCAGACACTTAAACAGAGCTGGCTACTGGTGACTCAGTTTAACAGAGAAAGCAGTATCTCTATATGCACTCTGAAGGAAGCTTGATGTAGCATGAATTTAATGTGTACTTGGGATGCTGCAGAGGGCATACAGGTGAGCAGGCAAAGTCTGATGTTGACTCCTTTGCTCATTTACTCAACGGTCACTCAACAGCGTGACCCTACTCACTTCCAGGCAGCATTGCAACCGCTGGCCCCTGATCTTCTGAAGATGAGATGATCTTTTAGAATCTGCTTGTGGTCCTCTAGTGAGGACACAGACAAAGCAGTAGGACCTGGGAAGTGAGTGTGACACCTCTGGGCTTAAGTGGGGAGGCAGCATCTCTAGCAAGTGAGAATAAGGCAAGAATAAAAGCTGGATTGTAGCTGTGGCCAGGACTTTTCCACAGTGTCCAAAGCGTGATCAGCCCCCAACACCGTGCACATGACTGCATCCCAGCACTTGGGTGTAGAGATGAGGATATCTCAAACTCAAGGTCATATTTGACTACATAAGGTGTTCAAGACTGTCTTGGATTACACAAGACATATCGGGATGGGTATAAGACAAGAGTAGGGGGGTCCCAGTCCTTCTGTTGCTCTCAAAGCCATTCAGAGGCCATAGCAAGGAAAATCCCAGCTTTCACTTTGGTAGAGGTTAAATTACCTTCAAGTTTCCAAAGCATCCCCTAAGATTCCTCAAGAGAATCATTGAAGTATAACTCTGGGATTTATAGTGGGGTACGGGGAGTGGGGGGAATTGGGAAATTTCTCTCTCTCTCAAAATGCAGCCGTATGCCCACAGAAAAGAGTTCCTTTAATTAAAAAAAAAAAGTATTTGGTTCTATTATGCAAATGACTAAGATGTGTGTGTGTGTGTGTGTGTGTGTGTAGTATAAGCAGGTACAGGGTCTCAGGATCGAGTGCATTATTAGAAAAGTGTTAATGCTTCCCCTTATTCCACCACCCCACCACCTCCCCCAACTCCCCACCCCAACCTCGCACCCCCAACCCCTCCCAGCAATGGACATAGAGACACCTTGTGGGTGTTTGTGCTCTTCTATTAGTCAGAAGCAGAGCAGATGGCAGTCCTTGGTTCACCCTAGCTCGATGCCTGGCTTGGTGGCCACAGCTGTGTTATACCTAGTACACATAAAAGTCAAATCAAACCAGGCAGCACACCACAGTACAGGAGGAGGAAGACAGACAGAGAACAGCAGACCACCATGTCACCCACAGATCTGCCCTCCAGCACAGGGCAACAGGCGGGCCATAGACATCTGCTTGGCACCACACAACACTACTTGGATGGAGGATGTCACCCAGGGGGCTGCCAGCAGCCGTGTGGCCAGCCTGCATCTGGGGCCACCACAGTGCATGAGTACATGGTGGTCAGACGGGAGAGGAAGAGAAGAGGAGCATTATGAAGAGAGTGGAACTGGATGCTGGACGGTAGAGAGAAGTAGCCTGTTGTGGGTGCCCAGTACTGCTACAGTCTCCATGTTCATGGCTCATATTACCAGTATAGAACATGGGGATGGCCCTGGTGGTTGAAGCCATATGGGACCACGTAGATGTTCAAAGGCTGTGCTAAACTGGTCACGCCCTTCGCGGTATACAGTTCTCTGGAGAGCTGGCCCCACTCTTCACTGGTTCACCAGCTGCAGCACTTGGGAGAGAAGCCCTGCACCTTGCCTGGGCAGCACAGTAGTGCTGGCCCTGGTGGATGGCGAGCAGGTGAGCCAGCCCCAAGGACAGAGCAAGATAGAGCTGGCTCCACCACTCATCTGCTGTGAAGTGGCATGGGGATGGGGGTGATGCCCCCACTCCTGCACCCCCCCATGCTCCTTGCTATCTGCTTAGTCTGGAAAATTGGCCCCCAGGTGGTGAGAGCTGGTGAGTTGACCCTGCCCTTCGACGGCTGCAGCACTTGGAAGAGCAGACCCTGCACCTTACCTGGGTAACACAGTGGAGCTGGCCCTGATGGCAAAGGCATGGGTAAGCCAGCCCTGAGGGCGTGAGAGCAGGAGAGTCGACCCTGCCTCCTGCCAATGATGGTACTGGGTAGCCTAGCCAGAGCAGTGCTGCAGAGCTTGCCCCGGTGGATAAGGGAAAGCCAACAGGTACCACCCAGACCCAGATCCAGGGCTCTGAATTGGCCCAACCCAAAGTCTATATCATCTGCGAATGGCTGGGACCGTGTGAAAGGGGCTGTATTACTGTCCCAAAGCTGCAGGATCTCCATGACACAGGACAGGTAACTGGAAGGAGTCTAGATGAGGATCCAGTATTGATGGTGTCACAGAAGCCAGAGATCTCAAAGCAGGCCAATGACTCATTGTAATGAGCATTTGCAAGTGAAGCTGTGTGGACAGAGGGACATACGGTGGGACACACTGTGACATACTACAGCTTCCACAACAATATATTTTCTATGCTTTGTTTATTGTTGCTATGGTGGCGGTGGTGGTGGTGGTGGTGGGTGTGTGTTTTATTTGTGGAGGGGGGTGCAAGGGCACAGGGCAGATATGAGGGGATGGGAGATGAACGGGACTGGGGTGCATGATGTGAAATTCACAAAGAACCAATAAAAAGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGGAAGGAAGGAAGGAAGGAAAGAAGGAAGGAAGAAAAAAGTTATGCATGTAGCTCAAAACAAATAGAATCTCGTGTTACAAATCTACTCCCTATACTTACCTGCCTCCTGGTAACACACAGAGACTTGATTCCCCATGCTAGGTATGTGGCTCAGTGGTACAGCACTTGCTTAGCATATTTGACACCCTGGGTTTGATTCTCAGCATGACAACGAAAAGAATTATAATTTCTCTATGGAGCTGGGTAAGACGCCTTCCAAGTCTGACCTCATTGGTACTTTTTCTTCCTGTTTTGTTTTAAACCAGAAAAAGACAGCGTTCTGAACTGGGCTGAGAAGCTTTGGCCCCATAAAGATTTGAGCCTGAGGGTGGCACTTTTCTTAGGATACTGTCCCCTAGTGGTAGAATAGGTCCCTGAGCATCACAGCAGCTGTGACTGGATGGGATTTCAGGTTTGCTGGGACAGAGATTGCATTTCAGCATCCCTTGGCAGCAGAATCTGGCCTAATTTTGATAAAGGACATTTCCAAAAGCCTGGGAGAGAGATGGAAAAGAACAAGGGAGATTGTTCCATGGAGTGCCTGTTGACCCATTAAACACGGCAGACAAGAACTACCTGCACAACAAGGTTCTTGCTTTTCATTTTTCTGGCACATTGGGACCAGGCAAGAGCTAATTAAATGCCTCCCCGTTTTGTAGGGCTGCCTGTCTCTGCCTTAGCCCTTCTTGCCCTGCGTTTCTGCTTGGCCTCACCCTGAGCTGGATACAGAAGTGGATTTTTTTTTCCTGCTTACTTGGGATGTGCCAGCTACTGGACTCCCAGCCTGACATTTTCTAGCTGCTGAGGTTTTCTCTAGCGTCTCCTATGCAAGAACTCAGCGCATCTCAGACTGGGCCATTTGCCAGAAATTAGAATGAATGATAGGACCTGGTATCGTTCGATGCAGAAAGGGTAGCTCTGTGTCCCTAAAGACTAACTTCTAAATAACCGCCTGAGGATGCTGACGGTATGCTGTGGAATTTTTTTTGAAAGAAGAAAACTACTTTCTTTGATGCCGGCATTGTTTTTTAAAGCAATATTTTTTTCAATCAACTACTATTTCCAAGGTAAATGGGCTGAGAAGTGTTTTGTAAGGGGGTCTTGTGGCAAACTAGAACGATTGGTGTCCTGCCTGCTTCCAAACGTGGGAGCAGCAAAGCAAACAAAACCACCCTTGGGGCCCAGATTTGGGCTGGGCAGGGCGAGGGCACAGGAGAGAAGGCAGGGAAGACAGGAAAGACAACTCATGGGCACTGTGTTCCCCCAGCTGCCCCGGCGCTGCCTGAGAGCTTGCTGAAGATAAAAATCAGCTAGCCCAGCGGCCCCATGAGATCAGCAAGGAAGGTAAAGCTGGCAGCAGATATCCCAACCCTGCAGCAGAGCCTGGATACAGACCCAGGCTGGGAACCCCGGGGCTGGAAGTGGGTCCACAGAGGCTCTCATTAGTGTTTAATGGAGGGATCAATCAATTATTCTGCTCTGCCTCGGAGTAGAGTACAGCGAGTGTGTCCTTAGATGTCTGCAAATTAGAGTCGTGGGGAGAGTGTCTAGAAATCTCCATGCCCTGACTATGCCCTAGATCACTCGCTAGAGGAACCGTGGTGGCATGGTGAAGCATATGTGTTATCTTGATGTGCAGCCAGCCCTCTCCTTTGACCTGATCAGAGAACTTCACTTATAAACGTGAGCATTTTAAACACAAGCACATCAAGAAAGAAGTGACTTAGATAAAATGGAGCGTTCGTAGGTCCCCTATAATAAAAGTCTAGAGACAGAGTAGGTGGCATGAGGCAGAGAAGGCACACAAGCAATAGGGCAAGCAAGATGACATTCTCATTCATCTCCTTCATCGCCAGGTGATCTAGTTCATTCTAGATCATTCTAGTTCATTCTAGTTCTTGCCGGTTCATTCTGGCTGCTGTAACAAAACAACATGGACTACGTGTCTTTATTGATCAGAGACATTTATTTTTTCACAGGCCTGGTGACTGGTCTAGGATCAAGCAAGACACCAGAGAGTTCAGGGTCTGGTGTTCAGGGCTCACTTCTTGGTTCAAAGACAGGATCCTCTCAGTGTAGTTGTCTACCGTGTCAGAGATGGGGTTCTCTAGGGCTTCTTTTGTGAGGAATCTAATCCCACGCTTCAAGGCCCTACCCTTTGGACACAATGATCTTCCCAAGTCCACTTCCTAACAAAACACCTTAAGAGTTAGGATCTTAATGCCAACCATGCTGGATTAGGGCCCACCCCAAAGACTCCAGGGGTCACTGGGGAACATTGAGGGACACAAGCATTCTTTGTATGGTTCTAGTCTCTGACTCCCCAGGACATGCCTCTCTAGACCCAGTATCTTGCTGGTTTCCCATTTTTTGTCCAGAGATGTCTCACATGATAAAGATGCTTCACGTGGCTAGGTGCCTGTTTTTATGCCTTTCCCTTCTTCCTGGCTGGGTAAGCACTGTCTCCTCCAAAGGTCCTGAGTCAAGACAGAATCTTGAACCCTTCAGAATACCATTCCTCTTTTCTCTTTACTCCTGGGACACACTCCCTGGGGCCAGGTATAGTTGCTTATTGTTGCCCAAGGATGCAATGATTTTTGCTGCCTAAGGCTGGCTTTGAGATTGCCCCCAGGTAGGGTACCTGCCTTTGTTTTGCCTGCTGGTCCATTACCTTCTTTGATGTTTTGCTCAAAATGCACCTCTTTTGGAAAGATAGAAGACCAAAGGGGGCAGGGAACTGGAAGGGCTACCTACTGCACAAAGTTTCACTTCCCTACAAAGCAGCAACAACAGTGATGCCCACAGCCCTGCCACCGTTCCACAGACATTTTCAGGCAGTGGGACCCTCTTGGAGCTTCAGGTGAGTTTGGGACGCATGAAACAACATAATGAAAATTTCACTTGGCTAGAGCCTGGGTTCAGGGACGCAGGGGGCTAATGCAGAGTGCTCTACAGCATGGGAGCAATGGAAGAGTGTACCTGAAAAAATGGAACCTAGAGAACTTACCACAGAGCTCCATTCTATGAATGTTGTCTTTCCTTCAACGTCATCACTAGTGAAACTGTAGTTACTCAGTTTCAAGTCACTTAGGCAATCTTCTAACGCACTGTTTCCTCCCAGTGACTCGGAAGATAATTGAGTGAGAGCCAGAGAGTTGTAGCTTTATTGTTCTTGCCCAAGCTAAAAAAGTGTTAAAACCTAGTGGTTATGGTAATGATTTGGGTTTTTGGAATGCCATGCTAAGTCAGTATAAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATTTCTATTCACATCCCTGAAATACAGCTATATGGCTATATTCATTCATTAGATGGGATTCAGAAAGACTCAGTAACTTTAAATTCACACAGCTGTAAGTGTTAAAAAAATGGAACCCGAAACCTTAGCTCACTAAGAACTGAGATAGGGTTTTACAAAAGATTTGAGTCTAATGATCCCATGTACAGAAAGAAGGGGACCTGGGGACTTTCATCCTTTCCAAAGGACCTGGAAAAGGAGGAAGCTGTTTCTGGCCCTCAGGGAGCTCCCGCATACAGGAGACAGGATTGTTCACACTGAGAATGTGTCTATGAAGGGGTCCTCCACTCGGAATGGCTCCTGGTATTAAAACACATCTTGATAAATCAGGATAGGGAAAACATTGGAGGAGAGAGAAAGAATTAAAAAATAAAATCTGTAAGCTGTTTCACGTTGATAAAGATTCTTTACCTAATGTAGAGTTGGACAGAGGAGCCAGGAGCCAGGGGTTGGGGTTTAAATAGAATGACCAGAGAAGTGGTGGCCTGTGAGCTAAGGCCTGAGAAGACACGTCCGGACAAAAGGAAGAGAAGATAGGAAGAACCCAAGGTGGAAGTGGTCAGAGAACAAGACCACTGTCACAGGAGACAAGGAAAAAAAGCCCCAAGGGAAAGTGAGAAAGGTATTTGGGGTAAACAGAGATTTCCCCTTGTAATGAGTTTGGCCTCTCCTCTAAGGGAGATTGAAAGCTTTCAAAAGACAAGGACAACTTGTCACTGGCTTCCTCAGGATCAACTGCAGGACACTGGCACAATTGTATTGGCAGTTAGGGTCTGAGGACAGTTTTGTTTCATATTTGTTATCCACCAGGATGCTGAAGTCACACTGGGGGGACAGGGAGGTTGACTGAAAGAGGACAGTGTGGTCAACACCCTGGCTGCTGGGATTTGGGTACAGCTATGCCACAGATGGCAAGTCGTACCATCTCAGCTTTTGAGAAGAAATTTCTCTGTTTAGAAAAGGAACTCAGGATACCAAATGATGATCTCGCTTTTGAAATGCTCAGAACTCAAGGTGTGAGTGAGTTCCATCGGAACCAGGGACCATTCCTGTGAGAACCAGGGACCATTCCTGTGAGCCAGAGTCCACCCACCAGGCTAGCTAGTGAAGAGGTGGCACTGGAGTGGGGGGTGAGGGTTTGTGGTGCCATCTTTCTTTTCTTAGACCCGGGCATCTGACCTGAATGATGTGCCCAGAGGCAATACAGATGTGCAAGACTGATGTCTCCTTTGTATGTGGAATGCCTGGGTTAGAATCCTAACTTGAGAACCTCAAAATGTGGTATTATTTGAAGAACCTCTTACAAAGTTAAAAATGAGATCATTGGAGTGGGCCCTAATCCAATATTTTTGATGTCTATACATGCCAAGATGCAGTTCCGACACAGAGGGAGAGCAGCCATGAACACTGGGGGCCGTACTGCCATTAGCCAAGGGTCTTCTGGAAGCAAGAACGATGTCTGAAGCAGAGAATTCCTTTGCACCTTCACAGACAGACAGACAGCTCCACCAACCTGGAGAAGCAGGATTCCCAGCTTCCAGAAGGGAGACTTTGTTTACTGATGCCTCCCAATAGCCATGGCAACTTTGTGATGCCTGGCTGGGCTCTATCAATAAGGCAAGCACCAAAGGAAATCCTGAAGGCAGCTTACCGAAAGCCACCACGTTAGCATCCAATGTAGACAGAGAAACAGAGCGGAAATGGTGGACTGAGAGGAGACAGAGCTTACACACCTCAGCAGAGTTGCCGCCATTCATCTAGGGCTTACGATGGAAGCCAGCAGACTGGCCTTTGGCGAGATTTGGGGTGTGCCTTTCGAAAAGGAGTCTCTGAAAGCCACAGCAGCACAACTGTGAACCCCAGGTTTTACAGAGAGGCTGGAGAATCAGGTGTTCAAGGTCACTCTCCACTCTGCAGCAAGTTCAAGGCCAGGCCAGCCCAGGCTGAGTGACACCCTCTCTCAAAACAAAACAAAACAAACAAACAAACAAACAAACAAACAACAATTAAAAAAAAAGTCTCTGTGGTGCTTTCTTTCCCTTAGGCATCTTTTGAGTTTCATGAAAAACAGAATTTTTGGAAAATAATCTTGATTAATTGGTATGACTAGTGTTAACATTTACTAATGGTATTGAAGGGGTCTGATCCTATATCCTATGGCTGGAACTGGCTGCCATCTTGCTTGCCAGCTTTGATTTCAGAATAAGCAGATCTGCCAAGTGACAACGTTCTGAGACCAAGAGAGGTCTGAGTGCCTCTTTGATTTTTTTTTTTTTTTCCTTTTTTAAACTAGTCAAGCCAGTGCTGCCGTTGTAGGTCTGTGTAGGCAAAAACACTGCTCTTTTCCCTTTGGCACACATGGTCCAATGGAGCAGAAAGGACACAGAGAAAGTAAACCAGAGCGAGGTTGTTGGCCAGTTCCTCCCCCTCTGCATCCAGTTGAACATGTGCCCACCATACACACAAAATGCATGTTTGGAAAAGAGAGGACTATATAATTAGAAAATAATTGGTACTTTTTTTTGGGCCTTTTTCATCAGATTCTATTGAAACCTTTTGCTATGGTGAAGCCCATCAGAAGCATGTCTGGGAGGCACAGGGACAGCAGATAACAGGAAATACAAACCAGCCATCGTCCGAGTGCTCGGCATAGGACCTCTCCAGGATGTGGCGCCCACTGGCAGGAAGTCTTCAGTCTCTAACCACTCTCATTAGTGAGGAGCCTGCTGTGGAAAATGCAGGCCTGTTTTTATAACACTTTCCTTTGTGTGATACACTCATCACAGAACTGTATATGGTCATTGTCAGGAGTCAAAGGCCCCCCCCTGGGAGGGCTCTCTAGATCTCCCTCCATTGTTGGGCCAGGGAGCGCTCAGGTATTTATAGTCCTGCATTATGGGAGACTCAACAGCAACTGGCTTGTTGCAAACTGAACTAAAGCAACAGCAACAGGGCTGGGTATTGTGAGACCCAGGAGCTGTGCCTTGCTGTAAGAAAACCTGCTTCATGATCGCCCTGTTGTTTGTGGAAGGAAAATAACACCTCATGGAAGACTGGGCAGCCACGTAGCCATCCTAGGTCATCTCCAAGAGCCTTGGCTGGTATGAAACTCACTGTGGTGCCCTGTCTGTCTCTGGGCCTCCAGAAAATTGCAAACTAGTTTTTAAAAAATCACACTATAATAAATAACATAGGCACATTGAAAACAAATTCCTATGCATTCGTGGAAGGGGGGAGGAACTGCTCCAGAAAGGTTCCAGCTCCCACCTCAAGATCTGATACAGACAGACAGACAGACAGCAGGCTCACATATGACTGGAGCTTCACATATCAAGCTTTTCAGTGCTGTCTTAACCACATATTTTGATATTATCTTTATAATAAGAAGTTGTTAAGTTGTTATCCAAGAAGGAATGGTGACCTGTCAAGGGGGCTCTTGTTGCACCCACATTTATACACACACAGATGAGAAATGAGAGATATGAGCGTGGATGAGTAAATAAACAGGGTGTGTGTGTG
GF3 -1 -1 1 CACCTGCAGACGGCGTATACCACGTAGC
GF4 -1 -1 1 GTCCACCTGCAGACGGCGTATACCACGTAG
EX52 -1 -1 1 CTTGTATGATGTGGTTTGTAAGAAGTGTC
EX32 -1 -1 1 GACACAAACACCATTGTCACACTCCACAGC
GR3 -1 -1 1 CAGTTGAGGAGGTCTCCAGGATGGCTGCTC
GR4 -1 -1 1 GTTGAGGAGGTCTCCAGGATGGCTGCTC
Comments:
Target: 0205Ensembl ENSMUSG00000065402 -- 65374230 -- 65374295 ENSMUSE00000522627 -- ENSMUSE00000522627
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:18:1:90772031:1, start 65408514: end 65408580
Found 10 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
REJECT (1) gap pair G5:18:65368730:65369729_842 G3:18:65376995:65377594_202
ACCEPT (0) gap pair G5:18:65368730:65369729_842 G3:18:65376995:65377594_538
ACCEPT (0) gap pair G5:18:65368730:65369729_842 G3:18:65376995:65377594_242
REJECT (1) gap pair G5:18:65368730:65369729_623 G3:18:65376995:65377594_202
REJECT (1) gap pair G5:18:65368730:65369729_623 G3:18:65376995:65377594_538
ACCEPT (0) gap pair G5:18:65368730:65369729_623 G3:18:65376995:65377594_242
REJECT (1) gap pair G5:18:65368730:65369729_636 G3:18:65376995:65377594_202
REJECT (1) gap pair G5:18:65368730:65369729_636 G3:18:65376995:65377594_538
ACCEPT (0) gap pair G5:18:65368730:65369729_636 G3:18:65376995:65377594_242
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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