High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by dk3
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 194369
Chr: 12
Strand: -1
Status: Ordered
BACs: RP24-211D19 : RP24-380A10 : RP24-395K12 : RP24-269I15 : RP24-162D10 :
Instance: Plate 149: Well F09: BACs RP24-269I15 RP24-380A10 RP24-395K12 RP24-211D19
Oligos on GRCm38:
Type Start End Length Seq
G5 110676122 110676171 50 TGTAATCAGCTTTTACACTACCCAAATAAATAGCTTTAATCTTAAACTAT
U5 110670552 110670601 50 TGCCAAGCTCACAAAGGAGCCCAGGCCTGGCCCCTGGTGGTCCACCACTT
U3 110670492 110670541 50 GTGAAGGGCACTAAACTTTCCTTCTCAACTGGCCCCCCCCAGGAATGCAG
D5 110668305 110668354 50 TGACCCTCTGAGCCATCTCAGAATCCGTACACCCATTTTTATGCTGGGTG
D3 110668197 110668246 50 GCACACACACATACACCATTATGTGTTCCTGTGGACGTTGGTTCTCTCCT
G3 110664260 110664309 50 TTTTAGTAACGTGGCATTTGTGTGAGCAGAGTTCAGAAGAGTCCCAGTGG
Oligos:
Type Start End Length Seq
D5_D3 111906457 111906514 58 TCCACCATGTGGGTCCTAAGGATTGAACTCCGGTCATCAGGCTTTGCAGCAAGCGCCC
G5 111914332 111914381 50 TGTAATCAGCTTTTACACTACCCAAATAAATAGCTTTAATCTTAAACTAT
U5 111908762 111908811 50 TGCCAAGCTCACAAAGGAGCCCAGGCCTGGCCCCTGGTGGTCCACCACTT
U3 111908702 111908751 50 GTGAAGGGCACTAAACTTTCCTTCTCAACTGGCCCCCCCCAGGAATGCAG
D5 111906515 111906564 50 TGACCCTCTGAGCCATCTCAGAATCCGTACACCCATTTTTATGCTGGGTG
D3 111906407 111906456 50 GCACACACACATACACCATTATGTGTTCCTGTGGACGTTGGTTCTCTCCT
G3 111902470 111902519 50 TTTTAGTAACGTGGCATTTGTGTGAGCAGAGTTCAGAAGAGTCCCAGTGG
G5_U5 111908762 111914381 5620 TGCCAAGCTCACAAAGGAGCCCAGGCCTGGCCCCTGGTGGTCCACCACTTTCCCACCAGCCCCAGGCCACTGAGGAAGGTCCTTTACATTTTCTTAATAAAAGAATACAAAGATGGGTGTCTCCCACACATACACCACACACACACACACACACACTTGCAGGGCACCTGAAGGCGATGGCGCGCGTGTCTCTGCCACTTCCGAAAGCCAGCTGCACTAAAGGCTGCCCTGATGCAGGTGGGTCGGCTGGGCCTCTGGCTGGCCATCCCTCAAGGCCAGGCACGTGGACTGTCACTTAACTAAGGAGATGAAGTAAAAGGACAAAGCGCGGGGTTCTAGAGCACAGGGAAGGAAGGGCAGGCCACAGGGAGAAGAAGGGAATTCTTTTTAGACATCTGGTCCCAACCTGTAGCTCAGTCTGCTCTGCCTCCAGATTCTCTGGGAATCGGAGGCAGCTGAAGCAGGAGGAATGTGAGCTCCAGGCTAGCCTGGGCTACATAGAAGGCCAAGGCCAGCTTGGGACATAGCAAGACCCTATGTCAAAAACAAAAGTAAGGGCTGTGGCTATAACTCGATCCATCCAGTGCTCACTTAGCACATGCTAGACCCTGCACTCGGCCCCAGCAGCTACAGAGGCAGGGGGATCAGAAGTTCAAGGCCATCCTTGTTAAGAGGCCAGCCTGGGCTACATGAGATCCTTCCTCAAAAGAAAAAAAAAAAAAGAAGAGAGAAAACCCCTAGGATTGTGGGCACATATAGTTCAGTGGTAGAGCGCTTAACTAGCTCTTGGGGACATCGAGAGAGCATGAGGGACGGTGCTCCACAAAGAGCCATCCTGGGTATCTGAGACTTTTTTTTCTCAAGACAGGGTTTCTCTGTGTAGCCCTGGCCGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCAAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCGTGCACCACCACACCTGGCTTGAGAACCTCTCTTTTAAAAGATTAAAATAAGGGCTGGAGAGATGGCTCAGCAGTTAAAAGCACTGACTGTTCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACAATTATCCGTAACAAGATCTGACTCCCTCTTCTGGTGTGTCTGAAGACAGCAACAGTGTACTGTGTGTAAGCATAAAAATAAAATATGAGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTCTGAAGGTCCCGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCATAACGAAATCTGATGCCCTCTTCTGGAGTGTCTGAAGATAGCTACAGTGTACTTACTTACATATAATAAATAAATAAATAAATCTTTAAAATAAAATATGAAAGTGCTAGAGATGGGTCTGGTGGGCTGGCTCAGTGGCTAAGAGCACTTGCTGTTCTCAGAGGACCCAGGTTTGGCCCCCAGCACTGCCTAGTGGCTCACAACCACCTGGATGTAACTCTAGCTGCAGAGGCTCTGACCCCCTCTTCTGTTGCACAGACATGAAGTCAGGAGACGCACTCACACGTCAAATATTCTTTTAAGTACCAAGAAAACTACTGCTACAAACCTCCGTGAAGTCTCATGTCATTCAGCGACTCCGGAACCCTCAGAGCAGCTGCCACACCCTGTTGGGAAGTGCCTCGTTACACACGTCACACTGGATGCTTTTCAGCAATAAATCCTGCCTTCCCAAACCGGGCTCGTGGGAACCCCATTCTGAAACCTGGAAAGCAAGCAAGAGACCCTAACCCTGCCACGCCCTCAGGAACTTGTGAGCATCATTTTTTTGTTTGTTTGGTTTGGGTTTTTCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTAGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCGTGCGCCACCACTGCCTGGCTGACTTGTGAGCATCTTAAAGGTTAGAAAGGCCTAACAGAAAAGGAGCCCAGCATGGCAGTGCGTGCCTCTGCCTCGAATCCTAGCACCAGGAATTCAAGGTCTTTATCATTTACATAGTGAGTTGGGCCAACCTGGGCTTGTGAGACCCTGCCTCAGAAAAAACGAAAATGGAAGAAGAGGCTATCGAGGTGGCTCAGAGGTGAGGCCCACGTACTGTTCCTACAGAAAACCTGAGTTCACTTCCCAGCACGCACATTCAGTGGCTCACAGCCGCCCTTCAGCTCCCGGACATCAGACACCTCAGTCTTAGGCATCTACACACATAAACATGCATACCTACAAACACTTTAAAATAACACTTGCTGGGCACGGTGGCGCACACCTTTCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGATCTACAAAGTGAGTGCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAAACAAACAAAAAACAGACAAGAGGGGCTGGTGAGATGGCTCAACGGGTAAGAGCACTGACTGTTCTCTGGAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCACAACCATCTGTAACAAGATCTAACACCCTCTTCTGGTGTGTCTGAAGACAGCTACAATGTACTTACATATAATAAATAAATAAATCTTTAAAAAATAAAATAAAATAAAAAAACAGACAAGAAAGGAGGGCTGGAGAGATGAGATGGCTCAGTGGTTAAGAGCACAGCTCTTCAGAGGACCAGGGTTCAATTCCCAGCAACCACATGGTGGCTCACAACCATCTGTGACTCTAGTCCTATAAGATCTGATCCCTCTCTGGCCACCACATGTGAGCACATGGGGTACATACAAAATTCCCATATACCTAAAAACTATTTTAAAGGAAGGAAGAAATAACCACGTGTACATTACCCAGACCCCTTTTCATCAAATGACCTTTTCTGCCCCCAACCCCAAGTGACATCTAACAGTCATACACTAATACACTGTGGGGTGACATCTAACCGTCCTGCTGTCTTTAGCTTTGAGGTTAGAGCAGCTTCTTAGAAAATCTTACTGGTTTGGGAATACACTGTGGGGTGGGTCCCCCCAAAGCTTTCATCTGGAATGGCCTGAGGCTTTCTTTGTGACAAACTGGAGTCATGGTTGGAGAGGACGACTCCAGAGGCCAAGTATCTGCGTGTCACATCAACATGACTTGGGTACTACAGCCTACAGTGGCTGGGTAGTGTCTTCCAGGGCCTCCGTTATGAAGTCTACCCCTATGCTGTCCTCTGGAAGTCAGCGTCACAGCCACATTGGAAAGGTGAGGGCCATCTACAGAGAGCAAGGATCCACATAACCATGAGCCCTGAGCTTCCCAGTGGTCCCTATAGACTGGTTTGGGTAAGTTCGGAGAGTGAGCCTAACACAGCCAAAGGGCTTCTCAGCCCCGTCCTGGCCCCACGTTATCTGATTGACACCAACTAAACTGTTTACAATCATACTATCATTATCATTTGATTTTCGAGGCAGGATTTTTCTGTGTGGCCCTGGCTGTCCTGGAACTCTGTCTGTAGACTGGACTGCCCTCAAATTCAGAGATCTGCCTTCTGAGTGCTGGGATTAAAGGTGACTGCCCGGCTCTTTTGTTTTATGTATAAGGATATGGTGCCTATACATGCCTGGTCCTTTGTACTCCACACACATGCACAAAATGCCCTTAACTACAAAGTCTACAAATGAGAAATGGCTCAGCAGTTAAGCACACTGGCTGGACCACCAGAAGTCCCATTTACGATTCCAAGTATTCACGTGGTAGCTCATAGATGCAACTTCAGTTTCAAGGAATCCAGCGCCCTCTTCTGGACTCTGAAGGTACTACATGCACACAGTGCACAGACAACCGTTCAGGCAAACACTCAAAATAAAAACCAAATGCCCCAGCACTTGGGAGGCAGAGGCAGCTCCTGTGAGTTTGAGGTCAGCCTAGTCTATAAGGTGAGTTCCAGGACTTCACAGAGAGACTCTGTCTCGAAAGCAAGCAAGCAAGCAACATTACCAACCTTATCATGCCTTGGGTTTCCCAGGTTTTAAAACTACAGGTACTGTTACGGGTAGACAAAGGCCCTGCCAGGCATCAAGGCCTCCAGCTCCTGCCCTCTGCTGTCTTCTCACTGCTGGGATTCTGCTAAGCAGAGGGACCTTGAATGGACGTAGAGAACGCTCAAGAGAGGAGGCGCTCTACTCCCGAAGGTGTCTCCTTAGGATGGAGGCCTTGGAGCCTGTGCATAGGAGCCTCTGGGCATTCCGTGCATGTGAACTGTCTGGCTCTGGTTCAAACACCCTGCCCCTGTCCCACCGTTAAGGGGACGGGTGGGTGACAAATCTGTAGCATGGGCTCTATACCTGCAGGACTCGATTCACTGAGAGGACACTTAGCTTACAGCTAGGGAAACTGAAGCTGAGAAAAGTGGACTTATTCAGTGGCAGAGTGAGGGCCAAAGATGACCGAGCTGGCCTGAAACTCACAGAGATCCACCTGCCTCTGCCTCCCAAATGCTGGGGTGAAGGGCATATGCAACCATGTCTGCTGTTAAGCCTTTTCTAAACCTACATCTTGCCTTCAGAAACTGAACTTTTGCTGGACCTCGAAAACAAGGTAATACTATACCCTGAGATCATTTAAAACTATGCTCTCGAGGAGCTGGAATGGTGGCTCAGTGGTTAGGAGCCTTGGCTGCTTTTTCAGGGGAACAGGGTTTGCTTTCTAGTGTGTATGTTGTGGCACACAATGGTCTGTAATTCCAATTCCAGGGGGAATCTGACCCCTTCTTCTGGCCTCAGAGAGTAAGCATGCACACACATAGTGTGCAGATATTTAGACAACATACTCATACACATAAAATAAGAATAAATACAGCTGGGCGTGGTGGCACCCAGCACTTGGGAGGCAGAGGCAGGTGGATTTCTGAGTTTGAGACCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACAAAAAACTATATATAAGAATAAATACAATCTTAGGCAGAGAGTGGTATATGCATTTAATTCCAGCACTCAGGAGCCAGAGGCAAGCATCAGAACTCTGTGACTTCGAGGCTAGCCCAGTCTACAGAGTGAGTTCCAGGATAGCCTGGGCTATGCAGAAAAACTGTGTCTTGAAAAAAAAATCTCAAAAAAGTAATAGAATGATACACTCAATTCCCCACTAAGTAAGCAGCCTGTCTAGTGAACCTACCTTTGAAAACAACATAACGGACCCTGCTCCACCTCGAATGTACCTTGTAATCAGCTTTTTTTTAAACATTTATTTATTACTATATGTACACTGTAGCTGTCTTCAGACACACCAGAAGGGCATCAGATCTCATTACGGATGGTTGTGAGCCACCTTGTGGGTGCTGGGATTTGAACTCAGGACCTCTGGAAGAGCAGTCAATGCTCTTAACCACTGAGCCATCTCTCCAGCCCCTGTAATCAGCTTTTACACTACCCAAATAAATAGCTTTAATCTTAAACTAT
U3_D5 111906515 111908751 2237 TGACCCTCTGAGCCATCTCAGAATCCGTACACCCATTTTTATGCTGGGTGGTAAGGACTGAGTTTGGCGTGGGTCCCTGGCTCTGTGCTGGCCCCACACCCCTACCCTGAGCTAACTCGGGCCCCAGAGCTGGGCCACGTACTTCTTCTTCCTCTTAATTCTCTCAGGCTCTCCAAGTAGGAGCTCCAGTTTTCTCTTGGAAAGCGACAGCCTGGTCCCCTTATCTCTGTCACTCCCGAGTCTGGGTGGTGACCGGGTGGCTCTGGCTCTGCCCAGCAGCTGTCTGCCTATCTCTTGGGTCTCCACAGGATTGAGGGGGCTGGCAGCCCTTGTCATCCTCCAGTCCTCTGGTGATGGGGACAGTCTGCCAGGACACCCGTTGGTCTTTAGTTCACCCTCAGGCTGCATGTCGACGTCATGGACTTCGAGGGCTGCAGGGGCCCAGACAGAAGCTTTCATGGTGAGGTCTGGAGCCTTTGGGAGCGAGTCTTCCACGCTGGCCTCCTCAGCAGTCCTGAATTTCAGTGTGTAGGAAGGCGAGTTGCGGTTCCTATCACCCAGGAGGTGGTGGGCACGATGGCTATGGTAACTGCGATAGTTGGAATGACCTGGGGTGTCTTCCGAGTCACCATCATAAGGATCTAAAAACTGGTGACAAAGGTTTATAAATGTTTGGCTACGTGTGAGGGCCTGAAAATTCTAATCACCACAAAATAGAAAAGTTTCTGAGCCTGTGGGTGAATCCCAGCAGCAGCGTTGTAACACGGAGGTGTCCCAAACGTGCCTCCATCACTGTGGTGGCAAATGGCAAATCTGTATGCTTTGCCTTGCATTGCAACTCTCTTTATAGAGGACAAGGTCTCGAATTCAAGACTGGCTTAGAACTAACTGTGTGGCCAGGATGACCCTGAAACTTCTGACCCTCCTGCCTTCACCTCCCATCAAGGGACCATTCTGAGGGTCAGACCCAGATGCTCGCAAATGCCGGGCAAGCATTCTACCAACTGGGGCTGGGCAAGCATTCTACTCACTAGGCTACATTCACAGCCAGTGCTGTCAATGGCTTTTGGGTTGGGTTGGGTTGGGTTGGGTTGGGTTGGGTTGGGTTGGGTTGGGTGGTTGGTTGGTTGGTTTTGAGATTGTCTCACTATATAACCTTGGCTGGCCTCAAACTCACAGAAATCCACCTGCCTCTGCCTCCCAAGTGCTGGGATCAAAGGTCTGTACCACCGCACCTGGCGTGTTTTGCTCTTTAAGGTCTCAGGGCTGTGGAGATAGGTTAGCAGTTCAGTTTCCAGATTCAATTCCTAACAGGCACATGATAGCGCACAACCATCTGGAAGGCCAGGTCTAGACCATCTGTTGCCCTCTTCCAGCCTCCTTGTATACTGTGCATGTGTCACAAAGTACTCAGACATAGAGAAATAGATCATTCCAAAACATTAAAAGGCAGGGTCTCATGTAGCCCTGGCTGCCCTTCAACTTTACATCCTCCTGCTCAGACTCCCCGGTGCTGGGTACAAGTGTGCAGCAAATTCCTCGTAAAAGTACTTGGGCAGGGCCTTTGGGGAGTGCCCTGACCTCTGCTGTGGGGTTCATACCTTCCTATCTTCTTGCTTTCTGTGGTAGAATCTCGTCAATTTGCTCCGGTCTCTTCCTGCCTCATAGCATCTGTCATCCCAGCACTCAGAAGGCAGAGAGAAGGGAAGTATGTCCTGAGTTTGGGGCCAGCCTGGGCTAATGCAGTGAGGCATCCCTCCTTTAGTTACCAATACCAGACTGCCTGTGTGCAGGCTCGCTCACACAAGTAGAAACATTCCCTCCTTGGATTCTGACTCTCACGGCCCACTCACTGGGCTAGCCTTGGCTTTGAGGCCTCCCTTAGGCTCTGGACCCTAGGGGGTTGCGGCCAGGCCCATGGCATACCTCTCTCCAGATGCCTTCAGCAAGGTCCATGAGGTTCCTTCTGGAGCTGACCTGTGGGGAAACACTCGATGCCGATTCTGCCATGCATGGCAGCTTAACACAGAGCACTTGGGGTGCTCGCTGCCACACTACAGACGCTAGCACGCAGCCTGGTAAGAGGGGAACGAAGTCCTCCAAAAGGCTAGTGGCCAGGCAACCCTTCCCTGGCAGAGTGGGCACACAGCAACTTGGCACTGGTTCTGGGTCCAAATCCAGAAGGAGGTGAAGGGCACTAAACTTTCCTTCTCAACTGGCCCCCCCCAGGAATGCAG
D3_G3 111902470 111906456 3987 TTTTAGTAACGTGGCATTTGTGTGAGCAGAGTTCAGAAGAGTCCCAGTGGCCATCACAAACCCCTAGCAGGGTCTGCCTTTAGGCTGAGTGAGGCTTCTGAGGCCTGCAGGCTACAGCCTCACAGCACACAACCAGGCCATTGGGTCTGGCTTTGTCTTAATTGCCCTGGGGTAGACGTGTGGCACGAGGACAGCCTACAGGCTGCCGTGGTGAGCTGATGGTCAAACAGCTCTTGGCCTGTCCACTGAAGTCCTCCCCACCCGGGCCTGGCCTAGGCGTGGACATGATCAGCAAGATGCTGAAGATGCAGATGCTGGAGGACGAGGATGACCTGGCCTACGCAGAGACGGAGAAGAAGGCAAGGACGGACTCCACCTCCGATGGCCGTCCAGCCTGGATGAGGACACTGCACACCACGGCGTCCAACTGGCTGCACCTCATCCCACAGACACTGAGCCCCCTGAAGCGCACCGTGGAGAACATCAAGGTAGCTGGGCTGGGCTCAGCGTGCTGCTCCCCAGGCAGGCAGATCTCTGAGTTCGAGGCTAGCTTGGTCTACAGAGTGAGTTCCAGGATAGCCAGGGCTATATAGAGAAACCCTGTCTCGAAAACCCAAAATACAAAAAAAAAAGAATGGCCACCGCTTTAATTAAACTCTTTGAAAGCTGTGATCTGAGAGGGGCAGAGAAATTCATCAGGCCAGGGCCACACTGCACTCTGGCCAGCTCCCAAAAAGGTCTGGTCAAAAGCTCTGGTGCTGCTCCCAGGTGTGCCTTGTCTCTGCACAGAGCCCGTCCTTAGCCCTCTTCTCTCTGGCTTTAGGATCCTTTGTTTAGGTTCTTTGAGAGAGAAGTAAAGATGGGGGCCAAGTTACTCCAAGATGTGCGCCAAGATCTTGCTGACGTCGTTCAAGTGTGCGAGGGGAAAAAGAAGCAGACAAACTACCTGCGCACGCTCATCAATGAGCTGGTGAAAGGTGCTGATGGGGTGTGCGGCTGGGAGCTGGTCTGCCACTGTCCACACCCCATGTGCGGGCACACTGCAGTCCCCAGGCCACACGTGTCTCTTTCAGGCTTCCTCAATAGTGGGGGTCTTAGCAACGCCCCTGGTTTTCCCTAGGTTTTCCACAGGCAGCTCCTGTGACTTTCAGGGTTAATGCAGTGAAGAGGACAGAGGGGTGGGCGGGGCCTGGTCCAGACACACTGCACGCTGCAACACCTCTAAGCAGGCACCCCACACTACTGAGACAGCTTTGCCCAGGGCCTTCCTGGTTGTGGACAGAACACCAGGCGCAGCCAGGAGAGCGGGCTGTGGTGTGCAGATGCTGCCAGCCTGAGCCACGTCTGCAGAGGGGGACTTGGCAGAACTAGTGCTGAGGCCTGCGGCTCTCTTCCTTGCTGCAGGGATCTTGCCTCGGAGCTGGTCCCATTACACAGTGCCTGCAGGCATGACTGTGATCCAGTGGGTGTCAGATTTCAGCGAGAGGATCAAACAGCTGCAGAACATCTCACAAGCAGCTGCATCTGGTGGTGCCAAGGAACTGAAGGTGGGCATGCATGGCCCGGGGTAGGGGGGTAGGGTCAGGAGTGGCACTGATTGTCCTGGACTTGGGTAGAAAGTGTTCTTTCTCGATGGCACTAAGGACAACCAGGGCCTGTACCTGACCTGCTCTCCTTTCCACCGCACTGTTTGCACAGAACATCCACGTGTGCCTGGGTGGCCTGTTTGTACCTGAGGCCTACATCACTGCCACCAGACAGTATGTGGCACAAGCTAACAGCTGGTCCCTGGAGGAACTCTGCCTGGAAGTCAATGTCACCGCCTCACAGAGTGCCACCCTTGATGCCTGCAGCTTTGGAGTCACAGGTGAGCTGGCGTCTGATCTCTCAGCCTGGCCTTTCTGTGAAACCTGTTTCCAAATGAATGTACCCAAAGTGTCTGGGCAGAGCCGCCCAGCTGAGCCTGTGGGTGTCCTGGAAGCCGAGCTGTGTGGCTCTGTATGGCTTCAGCCCCTTCTCCACATCTCCTCACAGGTCTGAAGCTCCAAGGGGCCACGTGCAGCAACAACAAGCTATCACTCTCCAATGCCATCTCCACAGTCCTCCCCCTCACACAGCTGCGCTGGGTGAAACAAACAAGCGCCGAGAAGAAGGCCAGCGTGGTAAGTGTCGTGGCCTCGCTCTTTGGCTCTGCCGTGCTGGGACTCTACTTGTGTGCAAACAACTGCACCCCAGCCCTGGACCCCCATGGGAGCTTGTCCCTAGAGCTGATGAACAGTAAAGTCCCTCTGTCTGTCTCACAGGTGACCTTACCTGTCTACCTGAACTTCACCCGGGCAGACCTCATCTTCACCGTGGACTTTGAAATTGCTACAAAGGAAGATCCTCGCAGCTTCTATGAGCGTGGAGTTGCCGTTCTGTGCACTGAGTAAAGCTAGACTTTTCTCCAGTCCTCTTTCTGGAATAGGAAACTCCCAATATTTAACCTTCATTCATCCTTAACGGTGTGGAAGGGTTGATGGACTGGAAAGCAAGTGGTTGGTTCGCGTTGGCGGGTGACAGGTGCTGCGGTTGGAGACGGTGGGATGGGAGAAGAGAAGAGTGCGCTGTCCGAGGGCTGACCGAATGGCTGGGTTTAGTAAATAAAACACTAAGCATGACGGGCTCCCGCCTCTTGCCGCTCTGCTCAGTCTGCTGTGGGAATCCTTGCGTCCCAGCCCACCTCACCTTTGTCACCGTGGTCACAGATGCAGAAGCAACAGGGTGAGACCAGGAAAGCTGCACCTCCTCCCTGAGGCCTGGCAGCAACTCTGTTCGTGACTGACACCCCTATCCCTGGGGACCTTCTCTCTCTCAGACCTAAGCCCTGCCACCAGTCCATCCTCCCACACTTCCTTTCCACATGACCTGTGGTGAGAGAGTGGCCTCGTCCCACAGGAAGGGTGACAATTAGCCTGGTCCTAGCAACTCAGGATCTCTAAATCCCTCTGACTGTGTGGTACAGGGTGGGCAGAGGCTGTTGTCGTAGCTGTTAAGGACTCCTTAGCACAGAGAGGCACTGTCCTAACTCCCTTCTCTGGGAGAAGACACAGCAGGTTCCTCTGCAGGAGGAAAAGCCTGTGTTCTCTTAAGGAACCTAGGGGTGGTGCAGGTGACACGGCACCCACGGCTTCTTGCTCCGTCCAAACACCTCAGAGGATATTGCCTGAGTCTGTGGGGATCGCTGAGACCACGAAGATCACCCCAGCCCAATAACTAGACTTCTGCTCCCAGAATACCAGCCTATCCCCAACCCCTCCTACAATCAGAGGGATCGTTCTGGACAGTGGCCACCAGCCCTGTTGTCCTCCCTGGCAGTGCCACCTGGGGCACTGATGAGTACCCATAACCAATCCTGGAACGGCCCCAGCAGCACCCAGAGGTGGACATGCCCTGCCAAAAACGGGCACCTCCTTGTGGTGCCGTTTTTATTTGCACTTGGGAACCTTGTCCCCACCGAGGGGAGGACAGGACCTGGAGAGCAGCCCCAGACGTGTGCTCAGCTTTGTTACCTACCAGAAAGAAAGTGAAAGGTCAGTAGACAAGCTGGCCGAAGCACAACTGGGGAGAAACAGCTCCCAGGAGAGCCCAGCACGACCAGAAGCCTGCTCCCAGGGGACACAGAGGGCTTGCCCAGCACGGGCAGTGAGAGAGCTCTGAACACACAAGGAACCGGAAGGGATCTGCCTTCGATCCAGCACTCAGGAGATGGGCAGGCAGAATGGCAGGAAATGGAACAAGATCCTGAGGTTTGGGGTAGGAGGCCCAGTGAAGGCCACTCGGGTCTATCCCTGAATTGCAATCTATTTTTAAATATTTTTTTACATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGTGCACTCGCGCACATACCCGTACACATGCACACACACATACACCATTATGTGTTCCTGTGGACGTTGGTTCTCTCCT
U5_15 111908762 111923812 15051 TGCCAAGCTCACAAAGGAGCCCAGGCCTGGCCCCTGGTGGTCCACCACTTTCCCACCAGCCCCAGGCCACTGAGGAAGGTCCTTTACATTTTCTTAATAAAAGAATACAAAGATGGGTGTCTCCCACACATACACCACACACACACACACACACACTTGCAGGGCACCTGAAGGCGATGGCGCGCGTGTCTCTGCCACTTCCGAAAGCCAGCTGCACTAAAGGCTGCCCTGATGCAGGTGGGTCGGCTGGGCCTCTGGCTGGCCATCCCTCAAGGCCAGGCACGTGGACTGTCACTTAACTAAGGAGATGAAGTAAAAGGACAAAGCGCGGGGTTCTAGAGCACAGGGAAGGAAGGGCAGGCCACAGGGAGAAGAAGGGAATTCTTTTTAGACATCTGGTCCCAACCTGTAGCTCAGTCTGCTCTGCCTCCAGATTCTCTGGGAATCGGAGGCAGCTGAAGCAGGAGGAATGTGAGCTCCAGGCTAGCCTGGGCTACATAGAAGGCCAAGGCCAGCTTGGGACATAGCAAGACCCTATGTCAAAAACAAAAGTAAGGGCTGTGGCTATAACTCGATCCATCCAGTGCTCACTTAGCACATGCTAGACCCTGCACTCGGCCCCAGCAGCTACAGAGGCAGGGGGATCAGAAGTTCAAGGCCATCCTTGTTAAGAGGCCAGCCTGGGCTACATGAGATCCTTCCTCAAAAGAAAAAAAAAAAAAGAAGAGAGAAAACCCCTAGGATTGTGGGCACATATAGTTCAGTGGTAGAGCGCTTAACTAGCTCTTGGGGACATCGAGAGAGCATGAGGGACGGTGCTCCACAAAGAGCCATCCTGGGTATCTGAGACTTTTTTTTCTCAAGACAGGGTTTCTCTGTGTAGCCCTGGCCGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCAAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCGTGCACCACCACACCTGGCTTGAGAACCTCTCTTTTAAAAGATTAAAATAAGGGCTGGAGAGATGGCTCAGCAGTTAAAAGCACTGACTGTTCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACAATTATCCGTAACAAGATCTGACTCCCTCTTCTGGTGTGTCTGAAGACAGCAACAGTGTACTGTGTGTAAGCATAAAAATAAAATATGAGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCTTCTGAAGGTCCCGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCATAACGAAATCTGATGCCCTCTTCTGGAGTGTCTGAAGATAGCTACAGTGTACTTACTTACATATAATAAATAAATAAATAAATCTTTAAAATAAAATATGAAAGTGCTAGAGATGGGTCTGGTGGGCTGGCTCAGTGGCTAAGAGCACTTGCTGTTCTCAGAGGACCCAGGTTTGGCCCCCAGCACTGCCTAGTGGCTCACAACCACCTGGATGTAACTCTAGCTGCAGAGGCTCTGACCCCCTCTTCTGTTGCACAGACATGAAGTCAGGAGACGCACTCACACGTCAAATATTCTTTTAAGTACCAAGAAAACTACTGCTACAAACCTCCGTGAAGTCTCATGTCATTCAGCGACTCCGGAACCCTCAGAGCAGCTGCCACACCCTGTTGGGAAGTGCCTCGTTACACACGTCACACTGGATGCTTTTCAGCAATAAATCCTGCCTTCCCAAACCGGGCTCGTGGGAACCCCATTCTGAAACCTGGAAAGCAAGCAAGAGACCCTAACCCTGCCACGCCCTCAGGAACTTGTGAGCATCATTTTTTTGTTTGTTTGGTTTGGGTTTTTCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTAGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCGTGCGCCACCACTGCCTGGCTGACTTGTGAGCATCTTAAAGGTTAGAAAGGCCTAACAGAAAAGGAGCCCAGCATGGCAGTGCGTGCCTCTGCCTCGAATCCTAGCACCAGGAATTCAAGGTCTTTATCATTTACATAGTGAGTTGGGCCAACCTGGGCTTGTGAGACCCTGCCTCAGAAAAAACGAAAATGGAAGAAGAGGCTATCGAGGTGGCTCAGAGGTGAGGCCCACGTACTGTTCCTACAGAAAACCTGAGTTCACTTCCCAGCACGCACATTCAGTGGCTCACAGCCGCCCTTCAGCTCCCGGACATCAGACACCTCAGTCTTAGGCATCTACACACATAAACATGCATACCTACAAACACTTTAAAATAACACTTGCTGGGCACGGTGGCGCACACCTTTCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGATCTACAAAGTGAGTGCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAAACAAACAAAAAACAGACAAGAGGGGCTGGTGAGATGGCTCAACGGGTAAGAGCACTGACTGTTCTCTGGAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCACAACCATCTGTAACAAGATCTAACACCCTCTTCTGGTGTGTCTGAAGACAGCTACAATGTACTTACATATAATAAATAAATAAATCTTTAAAAAATAAAATAAAATAAAAAAACAGACAAGAAAGGAGGGCTGGAGAGATGAGATGGCTCAGTGGTTAAGAGCACAGCTCTTCAGAGGACCAGGGTTCAATTCCCAGCAACCACATGGTGGCTCACAACCATCTGTGACTCTAGTCCTATAAGATCTGATCCCTCTCTGGCCACCACATGTGAGCACATGGGGTACATACAAAATTCCCATATACCTAAAAACTATTTTAAAGGAAGGAAGAAATAACCACGTGTACATTACCCAGACCCCTTTTCATCAAATGACCTTTTCTGCCCCCAACCCCAAGTGACATCTAACAGTCATACACTAATACACTGTGGGGTGACATCTAACCGTCCTGCTGTCTTTAGCTTTGAGGTTAGAGCAGCTTCTTAGAAAATCTTACTGGTTTGGGAATACACTGTGGGGTGGGTCCCCCCAAAGCTTTCATCTGGAATGGCCTGAGGCTTTCTTTGTGACAAACTGGAGTCATGGTTGGAGAGGACGACTCCAGAGGCCAAGTATCTGCGTGTCACATCAACATGACTTGGGTACTACAGCCTACAGTGGCTGGGTAGTGTCTTCCAGGGCCTCCGTTATGAAGTCTACCCCTATGCTGTCCTCTGGAAGTCAGCGTCACAGCCACATTGGAAAGGTGAGGGCCATCTACAGAGAGCAAGGATCCACATAACCATGAGCCCTGAGCTTCCCAGTGGTCCCTATAGACTGGTTTGGGTAAGTTCGGAGAGTGAGCCTAACACAGCCAAAGGGCTTCTCAGCCCCGTCCTGGCCCCACGTTATCTGATTGACACCAACTAAACTGTTTACAATCATACTATCATTATCATTTGATTTTCGAGGCAGGATTTTTCTGTGTGGCCCTGGCTGTCCTGGAACTCTGTCTGTAGACTGGACTGCCCTCAAATTCAGAGATCTGCCTTCTGAGTGCTGGGATTAAAGGTGACTGCCCGGCTCTTTTGTTTTATGTATAAGGATATGGTGCCTATACATGCCTGGTCCTTTGTACTCCACACACATGCACAAAATGCCCTTAACTACAAAGTCTACAAATGAGAAATGGCTCAGCAGTTAAGCACACTGGCTGGACCACCAGAAGTCCCATTTACGATTCCAAGTATTCACGTGGTAGCTCATAGATGCAACTTCAGTTTCAAGGAATCCAGCGCCCTCTTCTGGACTCTGAAGGTACTACATGCACACAGTGCACAGACAACCGTTCAGGCAAACACTCAAAATAAAAACCAAATGCCCCAGCACTTGGGAGGCAGAGGCAGCTCCTGTGAGTTTGAGGTCAGCCTAGTCTATAAGGTGAGTTCCAGGACTTCACAGAGAGACTCTGTCTCGAAAGCAAGCAAGCAAGCAACATTACCAACCTTATCATGCCTTGGGTTTCCCAGGTTTTAAAACTACAGGTACTGTTACGGGTAGACAAAGGCCCTGCCAGGCATCAAGGCCTCCAGCTCCTGCCCTCTGCTGTCTTCTCACTGCTGGGATTCTGCTAAGCAGAGGGACCTTGAATGGACGTAGAGAACGCTCAAGAGAGGAGGCGCTCTACTCCCGAAGGTGTCTCCTTAGGATGGAGGCCTTGGAGCCTGTGCATAGGAGCCTCTGGGCATTCCGTGCATGTGAACTGTCTGGCTCTGGTTCAAACACCCTGCCCCTGTCCCACCGTTAAGGGGACGGGTGGGTGACAAATCTGTAGCATGGGCTCTATACCTGCAGGACTCGATTCACTGAGAGGACACTTAGCTTACAGCTAGGGAAACTGAAGCTGAGAAAAGTGGACTTATTCAGTGGCAGAGTGAGGGCCAAAGATGACCGAGCTGGCCTGAAACTCACAGAGATCCACCTGCCTCTGCCTCCCAAATGCTGGGGTGAAGGGCATATGCAACCATGTCTGCTGTTAAGCCTTTTCTAAACCTACATCTTGCCTTCAGAAACTGAACTTTTGCTGGACCTCGAAAACAAGGTAATACTATACCCTGAGATCATTTAAAACTATGCTCTCGAGGAGCTGGAATGGTGGCTCAGTGGTTAGGAGCCTTGGCTGCTTTTTCAGGGGAACAGGGTTTGCTTTCTAGTGTGTATGTTGTGGCACACAATGGTCTGTAATTCCAATTCCAGGGGGAATCTGACCCCTTCTTCTGGCCTCAGAGAGTAAGCATGCACACACATAGTGTGCAGATATTTAGACAACATACTCATACACATAAAATAAGAATAAATACAGCTGGGCGTGGTGGCACCCAGCACTTGGGAGGCAGAGGCAGGTGGATTTCTGAGTTTGAGACCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACAAAAAACTATATATAAGAATAAATACAATCTTAGGCAGAGAGTGGTATATGCATTTAATTCCAGCACTCAGGAGCCAGAGGCAAGCATCAGAACTCTGTGACTTCGAGGCTAGCCCAGTCTACAGAGTGAGTTCCAGGATAGCCTGGGCTATGCAGAAAAACTGTGTCTTGAAAAAAAAATCTCAAAAAAGTAATAGAATGATACACTCAATTCCCCACTAAGTAAGCAGCCTGTCTAGTGAACCTACCTTTGAAAACAACATAACGGACCCTGCTCCACCTCGAATGTACCTTGTAATCAGCTTTTTTTTAAACATTTATTTATTACTATATGTACACTGTAGCTGTCTTCAGACACACCAGAAGGGCATCAGATCTCATTACGGATGGTTGTGAGCCACCTTGTGGGTGCTGGGATTTGAACTCAGGACCTCTGGAAGAGCAGTCAATGCTCTTAACCACTGAGCCATCTCTCCAGCCCCTGTAATCAGCTTTTACACTACCCAAATAAATAGCTTTAATCTTAAACTATGCCCTGGCCCAGAATAACATGTGCATTTGTAATCTTTTTCTAAAATCAGAAGGCAGATGGAAACCAACCTCAAAAAAACAAAACAAAACAAAACAAAAAAACACCTATCTGGGGCTGGAGTGATGGCTCAGTGGTTAAATGCAATGACTGCTCTTCCAGAGGTCCTGAGTGCAATTCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGGGATCCGATGCCCTCTTCTGGTGTGTCTGAAGACAGCAACAGTGTATTCACATACATTAAATAAATTAATTTTTAAAAAACCCACTAATCTGCAGAGCATGGTGGTGTACAACCTCAATCCTAGCACTCAGGAGGCAGAGGCAGGTGGATATCTGAGTTCAAGGCTAGCCCAGTCTACATCATAAATTCCAGGCCAGCCAGGGCTACATAGTGAGAGACCCTGCCCCCCCAAAAAGAATAAATCTAAAACCCAAATATCCCATCCCTGTTAGATGTAGCACACAGGGATGGATAACAGCATCTGACTCCCGTTTTGAATGCTGTTTGGGGTCAGCTGGGGTCAGGAAAATCACATCTACCTTTGTCTGACCAAGAGCATCTTACTGCTTTCATACACCGTGTTTTCTGTTCACGAAGTACAGAGTGAAGCCCTTCGCTATAGACAGACTGACCACAGACTCCAGGACAGATAAAAGATTCCTAGCTTAAGATTTATTTATTTTCATGTGTGCTGGTGTCTTGCCTGCATGTGTGTCTGTGTGCGGGCACCAGATCCTCTGGGACTGGAGTTACAGATAGATGCAAGCTGTCATGTGGGCACTGGGAATTGAACTCAGGACCTCTGGAAGAACAGACTGCTCTTAACCGCTGAGCCATCTCTCCAGCCCAACTTTTTTCTTTTTATGAGACCAGCACACCAGTCCCCTCCATGTAAAGGCACAGCCCTAAGGATCTCCCAGCTCCAAGGCTCAAGCTGCTTCCCACACCACTAACCTCAGAAGGCAGAAACAGACCAAGAACGCGACCTGCGCCCACCTCTCTCTCGACCCTGTCCCCACCCTTAGCTTTTAAGCTGGAGAGTGATTTGAAGTCAAGTCTGTTATTGGCAAAACCACCATGCCACACACACACACACACACACACACACACACCTTTTTCTTTTAAAAAATTCTACATTGCAGGCTGGAGAGATGGCTCAGCGGATAAGAGTACTGGCTGCTCTTCCAGAAAACTGAGGTTCAATTCCCAGCAACCACATGGCAGCTCACAAACTATCTGTAACTCCTGATCCAGGGAGTCTGGCACCCTCACACCAATAAAACATAAAACAAAATTGTGTGTGCTGGTGTGTGCTTACCATGGTGCACCTGAGCTGTGGTAGACATGTTGGGGTCAGAGGGCAACTTCTGTTAGTCAGTTCTTCCTACCACAGTACTGAGGATTGACCTCAGGCCTGCACAGGGTAATGGCACTTTTACCCACAAGGCATCTCACCAGCACCCCTGCAAGGTCACTAGTAAGCCATCTCCTAAACCTCTCTACTCTGCTTTTACCCCACCCTGTCCCATCCCTAGGTCCCTTTGTCCCTGCACGAAGCTGCTTCAGGCCTCATTGCAGCTCCAGGGTGGCCCTGCAGCCCCAGAACTAGTAAGGCTTGCAGAGTCTCAGAGGCCTTATCTAGGGGCACTCTGAGGTGGGGGTGGGCTGGTGGGACAGGTTGAACCACTGCCTTTACAATACCACACTGCCTCACAATCCTTTTGTTTGTTTGTTTTTTGTTTGTTTTTGTTTTTGTTTTTTGTTTTTCTTTCTCGAGACACGGTTTCTCTGTGTAGCCCAGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTTGAACTCAGAAATCCACCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGTGTGCGCCACCACGCCTGGCTCGCCTTTACAATCCTTAGGGCTGGGAGGATAATAAAATATTTGCCTTTGCTTCATGTTTGAAAACTACATAAAGAACATTCTAGAGGTCAACACATCCTCCTGAAGTTACTAAGTCTTCACCTCCAGGACAATTCTATTTATTAATCTCCAACCCTGGGCACCTTGTGTGCTGCTCACTAAACATCTACCTAACCCCTGACAGGTCATGTGGCCCAAGATTGCCATATTACCCTGATCTCGTCTCCAATGGTATAGCCAGACCCACTGCCCACCTTCCCAGTGCTGTCTCCAGTGTGGACCTCTCCCTGACTCCCTTTCATTTACTCCAGCTGTCCTAGGCTTTTTTTTTTTTTCCCAATTTTAAACTGTTTTGTTTGTGTCTAAACTGGAGGCCCCATACATGGCTGCAAATTCCTAGCAGGTATGTTTTTGTATTTATTTTTTTAATGTTTTAAACATGATCTCCTATAGCCTAGACTGGCCTCAAATCTTAAACTATGTAGCCAAAGATACCTCCATCTTCCAAGTTCTGGATTATGGCATGTGCCACCAGACCCAACTTATGAGGTGCTGGGGATCAAACTCAAGCCCCCACACATGGCAGGAGGTACCCCAGCCAGTGAGCTACATCCTCAGCCCAACTGAGCTGTGCTCGCCAGCCGATGGCCAATGGCCAATGGCCTAGGGCTCCTGGAAGCCCTTGGTCTCAGGTTTCATCCCCTCCCAAGCACCCACACAGACACACACCACACAGGCAGAGCGTTCACATCCATCCGTCCGACACACGCAGCAGCATCCTCCTCCTGGATTTCCTTGAATTGTGACTGCATTGTCCACAGGCCTGTCCTCAAAGCCAGTCATAGCTCTGATGAACAGTCAGTGTACAATATCATGTTTGCTGGTTATGACTGCCATGGGCTCCACAGAGAACTCAAACCCAGTATATCCCAAGTCACCTGTCTTGTCTCCCAAATGTGCACCTCCATTCACACACAGCATCTCCTACTGCTAGAGTGCTCCCTCACACCAAGGAGGGACTCATATTTATTTCATTTATATGAGTACACTGTAGCTGTCTTCAGACACACCAGAAGAGGGTGTCCGATCCCATTACAGATGGTTGTGTGCCACCATGTGGTGGCTGGGAATTGAACTCAGGACCTCTGGAAGAGCAAGCAGCCAGTGCTTATAACCAATGAGCCATCTCTCCAGCCCTGTATTTATCTTTTTGAGACAGGGTTTCTCTGTGTAAACCTGGCTGTCCTGGTACTCACTCTGTAGACTAGTATTGTGGATTGTCTGTTTTTCCTGCCCCCTCAAAGAGGTGCATGTACGAAGGTGAGGTCAGCTGAAATCTTATTAGTCAGATAAGGCTAGACCTGTGATTGGGCAGTGAAAAAAGAAGGTAGAACTGAAAGTTTTAGAGAAGGGACACAGAGAGAGACAGAGACAGAGAGAGACATGGGTGGGAGGCAGAAACAGGAGAGACAAGACGGTACCTAAAGGAGAGGGTGACCAGCTAGAACCACGTGCCACGTGGTCCTAGGAGCCATAAGTAACTGGGGATTGCTTTGATAGACAATTGAGTAATGTAGGGCACATCTGTCCAATCTAGGTGTGCAGCTTGTATCTATATCAATTGAGTTACGAGTTCTTTGTATGGGCATTTTGGAGGTCGAGAATTTACTGTTATAAATCTGGATGGTAAATTATCAGCCTCTGTAGGCACATGGGAAGTCACAGGGGGCAGCAGGCCTTGGGAACCAGGGCATACCACCGTTTTGGGACTCCCCATGGAGGTGGGGAGACCTGCCAGCACATAGCTGGCTATAGCGTGGATTGTTTTATACAACTACACATTATAGACTAGGCTGGCTTCAAACTGAGAGATCTGTAGTGCCTCCCCAGTGCTGGGATTAAACGTGTGTGCCATCACCACCTAGCAGGAAAAAAAAAAAGCTTTAAAAAAGAAAAGAAAGGCTGGGTGTGGTGGCGCATGCCTTAAATCCCAACATTCGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCAAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACCCTGTCTCGGAAAAACAAAAACAAAAACAAACAAACAAAAAGAAAAAAGAAAAGAAGAGAAAAGAAAAGAACAGAACAGAACAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGGGCTGGAGAGATGGTTCAGTGGTTAAGAGCACCGACTACTCTTCCGAAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAAACATCTGTAATAAGATCTGACGCCCTCTTCTGGTGCATCTGAAGACAGCTACAGTGTACTACATATAATAAATAAATAAATCTTTTAAAAAGAAAAGAAAAGAGAAAAGAAAAGAGAAAAGAAAAGAAAAGAAAAGAAAGAAAAGAAAAGAGAAAAGAGGGCTGGAGAGATAGATGGCTTAGCGGTTAAGAGCACTGACTGCTCTTCCAAAGGTCCTGAGTTCAAATCCCAGAAACCACATGGTGGTTCACAACCATCCATAATGAGATCTGACTCTCTCTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACATATAATGAATAAATCTTTTTAAAAGAAAAGAAAAGAGAAAAGAAAAAAAAGAATCTTGGGGTTAGCCCCACAGCTGCAAGCTGGGTGGATCACCTCAGTCACTGGGGATTCATTTAAGCATTGGACACCTTCCTGAGAAAAGAGGGAGAGGAGAGTTGCTGGAGGGGTGGCGCAGGGGTGAGGAGTGGCTGCTCTCCCCGAGGACCCAGGTTCAATTCCCAGCACCCACAGGGCAGCTCACAACCATCTGTCCAGTTCCCATCTGACTCCCTCTTCTAGCCTCAGCAGGCACTGCACTCACATGATACACAGACAGACAGACAGACAGACAGACAGACAGACACTCAGACTTAAACAAAAAATAAAATAAAACAAAACAAAAAACCCTTCCTTGACAAATTTTTCTAGTGAATCTTTTTTTCAGGGAGCACAGATTATTATAACACCTCAGTAAAGAGCTACTAACTAGCGAACAAACTCAATGCAAACGATAAAAATGAGATGTCTGTCAACAAGGCAGCCGCAGGAACAGGCTGCGAGTGTTCCCAGGGGCCCCTCCCTTCTTGAGGAGGGCAGAGGGCAGCTGTTGCTTAGCCTGGTGATGTCATCTTTCACCCCCACCCCACCCCCAAACCCTGTCACAACCCCTGGGGCTGTGTCCAGAAAGACAAGCTGGAAACCTGGCTGCAGTCCCAGAAAGTGGGAGTGAATCTCGGGGCGCAATCTCCTGGGAGAAAGGTGGGCTTAGCCCAAAGAGGGATCAGAGCTCACATAGAGTGGCTTCCTCCGGCTGGAGAGATGGCCCAGTGGATAAGAGCACTGGCTGCTCTTCCAGGGGTCCTGAGTTCAATTCCCATGGTGGCTCACAACCATCTGTAATGGGATCTGATGCCCTCTTCTGCTGTGTCTGAAGACAGCTACAGTGTACTCATATAAATGAAAATAAATAAATCTTAAAAATAAAAATAAAAATGGCTTCCTCGCAGAGGAACTCAGCAAGTGCAAGGCAGACTTTGGTAAGGCTGCAAATGGGGCTTCAGTTCTGATTCCAAGTCAGTTTTGTCTAGAGGAAACTTCAAGTAAAAATAGTATTTTATTTTAGACTCCCCACCCCCACCCTGCCATGGCTTTGCATGTAGATGCTAATCCATCACGTTAATTCCAAGGTGCCCCAGAATTCTCCTATGGGTTCAAGGGGGAGGAGAGGGGTGCTCAGCTCTGGGCAGAGCCCCTGCTCTCTCAAAGCTGCTCAGCATTATGCATCGCCTGGCCCTTCTCAAACCCCAGTCTTCATGCTCAGGGCAAATGAGGAACCAACCCCTGTCATCTACCCACTTCACAACGAGGTTCCCTGAAACAGCAGAGCCCTGGAGAAGGCAGGAGGGCAGGGATACCTACTCCAAGGGGGTCACATCTTCATTGTCAAAGAATGACCTCCTCTTATCAGGGACCTTACACACACACACACACACACACACACACACACACACACACACACCCTATCCTGTCCTTTACAAGTGCCCTGTGGGGACAGTGGCTTCGGAGGTACATACCTGGTGGAACACATGTGACCACATCTGTTCAGAGATCCAAACCAGACCACACTTATTCTGTCCCCAAGAAAGTCGGCTCAAATTTAGGTGGTATAAATGACGAGGAGCCCCTAACAGTAGCTGGAGAGGGGCCACTGAGTTAGGGCTCTGTGAACCTGGTGAGGCAGCTAGAGCAATCCTGGGGCTAATGGTTATTTAAAGCTCATTGGCTCCAGGCTTCTGGTTTGGAGGACAAGGACTGCCATTCTAGACTTACCTCCTGGGTAGCATGTTCATTTTGGTCCCCAGGGAATCACTTGTGAGACAAGAAGGGAGGAGGCAGGTATCCTGTGCTTTAGGCCTGCATGATGTCACAGGTATCTCCATGGCAACCCAGTGTGACTGCTCCTGGGGCTGTGACTGGGAAGAGATCCACGTGTGCACTCCGGACCATAGGCCCTAAACCACAGTTCCCAGTTCCCCCCCTCAGTTCCCTGTGCCCCGAGTTCTGCTGCCTGCCTGCTTCACACAGCATGGGTAGTTGTCCCTATCCTGTGCATACAGGCCTGATCCTCAACAGCCACGGGGCTCCTCAAAGCCTTTCCTTCCTTGGGTGCAAGGCACTCTGCTGGGGTGCACTTAAGACCAGAGAGGCCACCAAGACTTCGAGGACTGTCTTGTGCCTTAGGATAGCTTCCAAATTCTCATTCTGGCCACACAGCCGGTTTTGTCCCACCCTGCCACCTAGTTCCTCTTATAGTCAGGTCACTAGGCCATCTCCCCTTCCAGTTGCCCACAGCTCTTCCTTCTGTGGGACACTAGGGTTAAAGGAGCCAAGCCGTCCCCTCTGCCATCTGTGCAGAGCTGTTTGCGTGTCATTATCTGGTTCCTCTTCTGCCATTCCCTAGCTGCCCTGGGGACTTTGAATCCCAATTTCTGAGCCAACTTGAACTCCCAAGACCCTCAAGCTATGGATGAGAAAGAGCGTCCCTTGGTCACCTGTGCCTTTAAAGGTGGTTCCCAGTCCCTGTGTATGCATGAGGACAGAGAAGCATTAAGGAAAAGGGAACAAGATGAGGGAATAATTCAGTAACAGGCAATGGGTTGGGAGCCTGGGGGAGTTTCTTGTCTCACTGACCCTTTGGTACCTCCGAAATACACAAATGGAAAGCCACACAGAGAAATCGTGCACACGTGGAGCCCAGTGCAAGACTCAAGGAAGGAAGCCATGTGTGGTGGCCATCCCAGGCAGGACTCAGAAGGTAGAGGCAGGAAGAGTCAAGGCCATCCTTGGCTACTTGGTAAGTTCAAGGCCAGCCTGGGCTACATGAGACCCCATCTCCACAACAAACACCTAAGGAAGTAAGAGTGGATTATTAAGGGACATAAAAGATCCCTTGAGAAACTGTATCTCTAAGCAAGAAGCTGGGACACCTGCAGCCAGTCATACCCAATCTGAGAGCCAAGACCAGCTGCAGACCAGCTGCAGTGGGCAGACACCCCTCAGGCCACATTCCAACATACTCAGAGGGCACAACCTACTGGTATTGGGTATTCTTTCTGTTTTTCCTTTATCCTTTTTTTTTTTTTTTTTTTTTTTTTTAAAGATTTATTTATTGATTATTGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGAGTCAGATCTCATTACGGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACCTTCGGAAGAGCAGTCGGGTGCTCTTACCCACTGAGCCATCTCGCCAGCCCCTTTTTTTTTTTTTAAGACTAGTCTTTCAAAAGTTATTTTAAGTGTTATGAGTGTCTTGCCCATGTGCCTGCCACTGGATGCCAGAAGAGGGTGTCAGATTCTCTGCAAGTGGGGTAAGATGGTTATAAGCCACCAGATGGATGCTGGGTACTGAACCTGTGTCTTCTGCAAGAACAGCACGTGGTCCTAACTGCTCAGCCATCTCTCCAGCCCTGTCTTTTATTCTTTTTATGCTAGAAATCTATGGCCCAGGACTGCAGAGGAAAGGCGGAAGAGTGCTTCAACCCTGGATACCTCAGATGAGGATAGCAGGAGTTAGTTACACACACACACACACACACACACACACACACACACACACACGCTTCCAACCTGACACCATGAAGCCAGCTGTCCCACCATCAACCCTAGTAGGAATTTAGTACTTTTGTTTGACCCTATAAGGCTCCCTCCTCCCCTGAGTCTTCCAACACTCCTTCCCTGGCCAATGATAATTGGCAGTCACACCAACCTTGTCCCAGTAACCCTGGCACCTCCCGGGGGCATAAATACCCCTCTCCCCACACCAATAAACCCAAAGCTGTTCCTGTGTTGTGTGTGTGTCCCTTGCCCTTGAACTCACCACTGACCCAAAAATTCTACCTGCACTCACTCGCCCCAGCTAATCTCTCCCTCCAGTCCAGGCGGGTGAGTGAGGGGCCTGACACTCCCAGAGTGGGAGAAGCAGATATGGGACGGGACGGCAGAGATCCAATTAGCACCTTACAAAGGCCACACACAAGCTCTACCGTTGAGTGGCACACCTGGCTGCCTTACACATCCCTTCTCTTTTTTCTGCATCTTTCATTTATTCTTTGACAACTGCGTCTGTGTATACAACACATTTTGATCTTATCCACTTCGACTCTTCCCTCCTGATGTCCCTCAACATGTCCCCCTCCCGCTCTTGTGACTTCGTCTTTGGCAACCCACCGAGTTTACCTAGTATTGCTGGCTGGAACATTAGCTGGCCTTCTTGGCTTGGTTGTCTAGGCTTTGTGCAGTCACGGGAGCTGTCCATACATGCGATTCTCTGTACTATGCAGAAGACAGTATTTCCCAGGACTCCCATTCTGCAGATCTTGTGTTCTTTTTGGTCCCTCTTCCCTATTTCCTGACCTTGGCTGCTGATACAGACGTCCTACTTAGGGCCAGGCTCTCAACAGTCACCTATTCCCAGTGCTTTGACAGTTGCGAGCCTGTCTGTATTGCTGCCCACAGCAGAAAGAGTCTGGCTAAGGCTGAAAGCAGCAGTAACCTGTGATAGAGGCATAAAGTTCAGCAAGTCCATCTAGCGGGACAGCAGTAGGCTCTCCCCGGGTCCTGTGACCTCCTAAGCCACAGGATTCTGACAGGGTTACAGTCCCAGGGATGACTTTTCTCGTGGGGAGCACACCTCAGATCCAATCAGAGAAGGATTGGTTGCCCCTGGAACCTTCACACAACTGTTGCACCAGAGGACACTTGTTGCCTGAAGTTGGTTATTGGA
D3_15 111891408 111906456 15049 TTCTGTTGAGTAATAAAAAGTATTTTACTACTGCATTTCAGTTTTGAAAGGTGCTGGCTTCTGTTTGGAACAGCTGTAGATTGTAGCAGATTATTGAGGCTTGCGCAGGCCTCAGCCTGTCTGTGGCTTGGGGTTGCTATCTGCCTCAGAAAGCAAAAATGGAGGCTTTATCTTCTAGGAGAACAAGTCATCTTCTCTGAAAAGTTCCCCTCTCCTATATGTGTTAAAAGAGAAAACAGCCGGGTGCGGTGGCGGGAGGCAAGGGTAGGTGGAACTCTGTGAGTTCAAAGCCAGCCTGATCTACAGAGTGAGTTCCAGGACAGCTAGCCTACATAGGGAGGACACTGTCTCAAAAAGACAAAAATAAAGTAAAATTAAAATTAAAAATAGAATGAGAAAATTACTGTTGTTGGTTGGAAATGTAATTCTTGTTTGTTTGGTTTTATTTTTGTTAAACTAATACAATTTATTTTAAAATACACAACTTTAAAATACACAACTCAGTATTTACATTTTTTAAGTCATCAAAATAATTTATGAGTTAACTGCCTCTTATATATACATGAAGCTTCTTCTGAAGCTAAAAGTAATATTCACTCAGCCAGTTTTTAAAATCTATTTGGAATATTAAACATGATAGAAGTAGAAGAAAATCTCTTATGAAGTCTTCTATGAAAGGAAATTATGACAAGTTTCTGATCAGACAGAAACCGTTCCATCACCAGTGGAGAATAGGCAGCGTACCTGGCTTCATAGAATGGGAAATGTAGTTCTTGAAACTATGCAGAGTGTTTCCAAGGAACAGCTTGCCTTAGCCTGGGGGAGGTCAGGGATGCTGTCTAGCAGACTCGGCCCTTCTCCAGCCTCTCTGAGTGGAGCGGGAGCCCTGGTGGTCATGGTTGGTCCTCACTTGGATGCTGTCTGTCTCCCCCATCCCACGCCTGCTGAGCAGCTGTGAAGTCCATCAAGAAGCAGCACCTGGTGGAGGTGAGGTCCATGGCCAACCCTCCTGCAGCTGTGAAGCTGGCTCTGGAGTCCATCTGCCTGCTGCTTGGTGAGAGCACCACGGACTGGAAGCAGATCCGCTCCATCATCATGAGAGAGAACTTCATCCCCACCATCGTCAACTTCTCGGCTGAGGAGATCAGGTGAGGCTGTCGCTGCAGCTGTGGGGGAGGCGTGAGCACATCTGGGAGAGGCTCTGCTTGTTGTACTCTGGCATGTCATCTACCAGACGGGAACGTGGTCACACTCATCCCACTGGTGCCTTTTCAGAATGCCTGAGAGAGAAAGCTAGCATTCTCCTCTGCATACGTTTTTGATATCACTTCTGCCAGCGGTCAGTTAAATCCTCCCGAGCTGTGAGTGCTTGCCCCAGCCGAGCAGGGCTCTTCCATCTGTTGCACCAGGACTGGCTGGAGTTACTGTAGTGAGGAGGGCAGAGCCAGTGTGCCTGTCCTCGTTGGCCCTGTCAGCTTCCTCTGAGAACCAGGGCACAGCAGCATCGTACGTGCTAGCCCTTTACTTGTCTTGCATGGAAACACATTTTTATTCTGTGCATGCTTAGAAATAATTCCCTCTCTCAGTGATGCCATACGAGAGAAGATGAAGAAAAACTACATGTCCAACCCCAGTTACAACTATGAGATCGTCAACCGGGCTTCCCTGGCTTGTGGTCCTATGGTGAAGTGGGCGATTGCACAGGTAGGTCCCTAAGCCAGCGCTGCAGGTGAGAGGCCACGGCTCTGTCACTTGGTTTCTACTTGCTCAGTGCTGCTGCTGTGGCGATTGACTGGGTTTTCTGTTTGGGTTTGGACTTAGTTACTTTTTGTTTTGTTTTGTTTTTTTTTAAGGTTCATTTACATTATTTTTTTTCCCACATCTGTGTGTGTCTGTTGTGGATGTGTGCACATGAGTGCAAGTAATTGAGGAGCCCAGGAAGGGCTCAGACTTCCTGAAGCTGGAGTGCAGGAAGCGGGGAGCTCCCTGAATGAGTGTTTGGGAAGGCCTTGCCTTGCTCCTCACTGCTGAGCCGGTCCTCTACCCCCCACAGGCTTGTGAGAGAGAGAGTCTACTTTATAACCCAGGCTGCTTCAGCCTCCGCACGCCAGGGTCACAAGCACAAGCCACCATCCTGGTTTCTGTTTATGTGGCTACACTAAGGCATGGGTGTCATTACGGCATGCCACACATTTCACCCTGTGCTTTGCCTTCCCAGCTCAATTATGCAGACATGTTAAAGCGAGTGGAGCCCCTGAGGAATGAGCTGCAGAAGCTGGAAGATGACGCCAAGGACAACCAGCAGAAAGCCAATGAGGTGGAGCAGATGATCAGGGACCTGGAAGCCAGCATTGCCCGCTACAAGGAGGAGTACGCTGTCCTCATCTCTGAGGCCCAGGCCATCAAGGCAGACCTGGCAGCTGTGGAAGCAAAGGTCAGCCTTCCTGCCACCCCATAGTCTCCATAGTTACCTGCGGGAAGCCAAGGTCAACAGTGCTGATGGGCTCAGCAGCATTGGGCTTGCTGAGGCAGTGAGCTTGGGTGTCTGTGTCTCTGGGTATACGTGTATGTCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTCGCAGTCCTGGCTTTGCACTCAGTACATTGGCTTGCACTCACTTCCTCCTTACTAGCCAGCCTGCCTTCCCTCCTGGCCACACCCAGCTGGTCCTCTCCTCAGGCTAAAAGTTAGCCTCTGCCTTTGGCTGGTACTCTGAAGAGATCCCAGCACCTGGGCCGCTTAGTGTCCACCACAGTCTCGCCTCGCCAGGACTCTGGACGGTCTGAGTGCTTTTAGTCCTCCTCAGGCCCTGCACACAATGTAGATCCTTTCTGTGTTCTCATTAATTTAAGGGTCAAGCATGTGCGGTCCATGCCACATGTGGAAACCAGAGGACACTTTTGTTAAGTTGGTCTTCTCCTCCCACTGTGTAGGTCTCAGGGATGGAACTCAAGTCATTAGGCTTGATGGCCTCTCAGTGACCCAGTGAATGAATTCCTTTGTAGTGTTCGGTGTGGTCCTAAACATGTCATTTGATTTCTTTGTCCTCCCTTAGGTAAACCGGAGCACTGCACTTCTAAAGAGTTTATCTGCCGAAAGGGAACGTTGGGAAAAGACGAGTGAAACTTTCAAAAACCAGATGTCTACCATTGCTGGGGACTGCCTCCTGTCGGCGGCCTTCATTGCTTACGCTGGCTACTTTGACCAGCAAATGCGCCAGAACTTGTTCACCACCTGGTCTCATCACTTACAGCAAGCCAACATCCAGGTAAGAGGCACTCAAAGAGAAGAGTCGGCCTGGGCACAGTTGCGCTGGCACAGTCTGACAGTCACAGGAGATGGATAACCAGTGTCCTCTTTCAGTTCCGCACAGACATTGCAAGGACGGAGTACCTCTCCAATGCTGACGAACGCCTCCGCTGGCAGGCCAGCTCTCTGCCTGCCGACGACCTGTGCACAGAGAATGCCATCATGCTAAAACGATTCAATAGGTGTGTGCTCTTAGGCCGAAGGTGCAGCTAGAGATTGGTGTGGGCGTCCTGTTAAATTGTACTCACACAGCTTGTTTGAAGAAAGGGAAGGAGAAGTGGTTGTGGGGAGGCAGTGCTAAGAGGTCAGCGTTTGTCTTTACATCTTAGTGAGTTCTGCTTACCACAGCTTTTCCGGGAGATATCTTGATTTAAGTCGCATCTGTTTAGGAGTCTGGCAGTGTGTAGAGATTGCCTGTCTGGAGTAATGGTCTCTTCCCATCCGATCAGGAGACTGACTTTCCTGATGGAGCTTTGACCTAATCACATGTCACTTTACCCTCAGGTACCCGCTGATCATCGACCCCTCCGGACAGGCCACAGAATTCATCATGAATGAATATAAGGACCGGAAGATCACAAGGACCAGCTTCTTAGATGATGCCTTCCGGAAGAATTTGGAGAGTGCTCTGAGATTTGGCAATCCCCTTCTTGTCCAGGTTTGTGTCTGTGTTTGAATTGTCACTCCTCCTAGGCGCATCTGTGGTTGCTGTGAAGTAAATGGAAATTACATAGCACAAACACCAGTAGAATTTATTTGAACTTTGTATTTGAATGGGGTTTGATGCTAAAACAAACACACAAACAAAAAAAAAAGCCAGGCCTGGTGGTGCACGCCTTTAATCCCAGCACTCAGAAGACAGAGGCAGGAAGATTTCTTAAGTTTAAGGCCAGGCTAGTCTACACAGAGTGCTCCAAAATAGCCATAAAAAGATCCTGTCTCAAAACCAATGACCAGGCAGTGGTAATGCACATCTTTAGGGAGGCAGAGGTAGGTACATCTCTGAGTTCAAGGCCATTCTGGTCTACAGAGCGAGTTTAGGCCAACCAGGGCTACACAGAGAAAGCCTGTCTGGAAAAAACTGGGTTTTTGTTTGGTGCTGGGGTGGAACCCGGGCCTCACACTACAAGAAAAGTGTTCTGCCACTCAGTCACATCCATGCCCCAACCCAACAACAATTTCTTTAAAAAGGCAAACCTCCTTTTTAGCTTCCTCTAAGGTATAAGGCAGGTCCCGGGCTCATGAGTGGTGCACTGGCCCGGATAGGAAGTTGATGGCTGTCTTCTGTGTGTGCCATACACAGGATGTGGAAAGCTACGATCCAGTTTTGAATCCAGTACTGAACCGTGAAGTTCGGCGAACAGGAGGGAGGGTGCTGATTACCCTCGGTGACCAAGACATAGACCTGTCACCATCCTTTGTCATCTTTCTGTCTACCCGAGACCCAACTGTAAGCAGTGAGACTCTTCCATAGATCACCATGATGCTGGTAGGTTTGGAGCGGAGAGAGCCAACCGGTCTGTCTTGTGTCCTGACAGGTGGAGTTCCCACCAGACCTCTGCTCCCGGGTGACCTTTGTGAACTTCACCGTCACCCGCAGCAGTTTACAGAGCCAGTGTCTCAATGAAGTGCTTAAAGCAGAAAGGCCAGACGTGGACGAGAAGCGCTCCGACCTCCTGAAACTGCAAGGTGTGCCTCTGGTCCCTGGCTTCCCAACCGAGTTGGGTAGCAGGCTGAGCTCCGGAGGGTGAGGGTGACACTGCTCCCCAGTGGCAGAGTGACACAAATCTGTCTTGTCACAGGGGAGTTCCAACTCCGTTTACGGCAGCTTGAAAAGTCTCTGCTCCAAGCTCTGAATGAGGTAAAAGGCCGCATTCTGGATGACGACACCATCATTACAACCCTGGAGAATCTGAAGCGGGAAGCTGCCGAGGTTACCAGAAAGGTGGAGGAGACAGATATTGTCATGCAGGAGGTGGAGACCGTGTCCCAGCAGTACCTGCCGCTGTCCACTGCCTGCAGCAGCATCTACTTCACTATGGAGTCCTTGAAGCAGGTGAGGCCATTGGCACAGCAGCTCTGGTTCCCAGCTGTGGCCCAAGGCACACTCTGATGGTCACATGGCTTCCTCCAGGTTCACTTCCTGTACCAGTACTCTCTGCAGTTCTTCCTGGACATCTACCACAACGTGCTGTATGAGAACCCCAACCTGAAGGGTGCCACCGATCACACACAGCGCCTCTCCATCATAACCAAGGACCTCTTCCAGGTAGAGCGTATAGGAGCTTGCTTCTGTCTGGTGCAAGGGTGTGGAAGTGTGGCCTCCAGACCACCACCATCCCCTCTCTCTCGCTTCCCAGGTGGCCTTTAACCGAGTGGCTCGAGGCATGCTGCACCAGGACCACATCACCTTCGCCATGCTGCTAGCGAGGATCAAGCTGAAAGGCACCGTGGGGTGAGGCAGGCTGCTCAGCAGTGATGTGGCTCTGCGGGCTGCCCTAGCATGCATTCTGTGTAAAGGCACCTGCCTGACCTGTCCCCCACTTCACAGGGAGCCCACCTATGATGCAGAGTTCCAGCACTTCCTCAGAGGGAAGGAGATTGTGCTGAGTGCTGGCTCCACCCCCAAAATCCAGGGCCTGACCGTAGAGCAGGCAGAAGCTGTCGTGAGGTTGAGCTGTCTGCCTGCATTTAAAGATCTGATTGCCAAGGTGCAGGCAGATGAGGTAAGTGTCCTTTCGAGTGTTCCGTGTGAGACCTGTCAGTGAGCAGCTACTGCATGCATGACTGCGTGGGCCCTAAAGGTCTGTTTATCTAATAGAGCAAGACCAAAGCTGCATGCCCAGGACCACACACCAATTATTCCTGTACCATGGTGACACTTAACCTATTGTCTCTACAGCAGTTTGGCATTTGGCTGGATAGCAGTTCACCAGAGCAGACCGTGCCATACCTCTGGAGCGAGGAAACGCCTACAAGTGAGTCAGAGCCAGCCCCGGCTGTCCTCTCTCAGGCCTGTAAGGTTGGGGTGTTGTTCCTCAGAAGGAGGCACTGATCCTTAGAGAGGTACTTGGGCATCAGTCAGGCTTGTTAGGAAGCCAGTCTGGAGTGTAGACAGACTCTGAGTCCCAGCTACTCAGGAGACTGAAGTAGGAAGGTTGACAGCTAGTTGGGCATGGTGGCGCACACCTTTAATCCCAGTACTTGGGAGACAGAGGCAGGTGGATTTCTGGTCTACAAAGTGAATTCCAGGACAGCCAGGGTTACACAGAGAAACCCTGTCTCCAAAAAAAAAAAAAAAAAAAAAGGAAGGTTGACAATTTAAGGCCAGCCTAAGCTAAATAATGAGCTCTTGCCTCAAAATAACAAAATCACCATTTTAGGAAACCATTTAGGGAGATGGCTCCTACGTTGGCGGAGGGAAGATCAGATTTCTGATTCCCCCTCACGTTATCACCAGTGACCATCAGAGGCTGGAGCGGGCTAGGAACATAGCTCAACTATAGAATACTTACCTAGCAGGCATAAGGTGATTGAGTTCATCTTATTGGAGTGAAAGCAATGCCTAACAAAGATGAGTGGGGATTATCTTTGTGTCATAAAGACTCTTTGAGTGCACCTGTGAGTGCCACATAGGTAAAGACCTGAGTTCCATCCCTGTGACCCATGTGCTGAGAGAACCAGCCCCACTAGTTGTCATGAACTGCATGTGGCCGCCACACATACACATGCAACATAAAAATTCCCCCAGGGCAGCCAACATAGCCCCACTCCATATAAAGAGACCTTGTCTCAAAGATGTTTTATTTACTTATTTTGCTAAGCATGATAACACATGCCTTTAATACCAACACTGAGGAGGCAGAGGCATGTAGATCTCAGAGTTCAAGCCTAATTTAGTGTGACTTCAATGCTTCCCACAGCTACATAGTGATATCCTGTCTAAAAAAATAATAATATACCCCACACCAGTGGTAAGACCTTACCGCCAGGGGGGAGTGGGTGGGTAGGGGAGTGGGGGTGGGTGGGTAAGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATACCTAATAAAAAATGGAAAAAAAAAAAAAAAAAAAGACCTTACCGCCAGGTATCTAATACGACATTGGAACAAAGACAGACGCTCCCTGGGTCCAAGAGAAACAGGAGGCTGGGGTCCGAGCTGGATCTCGGGGGCTTCGTGCCTGGCTGAGCTGGTGCTCTGGTGGAGATGTCAGCACCTTTACCCTCCCTTGCCACGCCTCGTTTCAGCTCCCATTGGGCAGGCCATCCACCGCCTGCTCCTGATTCAAGCCTTCCGACCCGACCGCCTGTTGGCCATGGCCCACATGTTTGTTTCCACAAACCTCGGAGAGTCCTTCATGTCCATCATGGAGCAGCCTCTCGACCTCACCCACATTGTGGGCACAGAGGTAATGGATGTCCTCGTGCCCCATCCCTGCTCCCCATGCACCCTGTGATATAGCCCAGACACACTGCTTCTTGGCTGCAGTCTTCCCCGAGCCAACACTACCCTCAGGCCTTCTCCTGACAGGTCCTGTCTTATAACCCGTCATCTGGGGTGTGCCATGTCATTTACAGGTGAAGCCAAACACACCTGTCCTGATGTGCTCTGTGCCTGGGTATGATGCCAGCGGACATGTTGAGGACCTCGCAGCAGAGCAGAACACGCAGATCACGTCTATTGCCATCGGTAAGGATGTGTGCTGGTTTCGTGAACACCGGGCAGCCTGGAAGCATAAGGCTGCCTGCCGTGGTTCACACTTGTCTTCCTTGTCTCAGGCTCAGCAGAAGGCTTTAACCAAGCAGATAAGGCCATCAACACCGCCGTGAAGTCAGGCAGGTGGGTGATGTTGAAGAACGTGCACCTGGCCCCGGGCTGGCTGATGCAGCTGGAGAAGAAGCTGCACTCCCTGCAGCCCCACGCCTGCTTCCGACTTTTCCTCACCATGGAGATCAACCCCAAGGTGAGCTGAGGGTGCTGGTGCCTCTGTCAAGCCTTTATCAGAACCAGTGGGTCAGCTGGGTGTGGTCACATGTCTGCAACCCCAGAACTTGTGATGCTGGGACAGGAGGATTGCTGCAAGCTTGAGGTCAACCACACATTGTCTCTCCAAAATAAAAGAAGACATCAATCACAGAGACTACCTTCTCCCTCAGGTGCCCGTGAACCTGCTCCGTGCTGGTCGGATCTTTGTGTTTGAGCCACCACCCGGAGTGAAAGCCAACATGCTGAGGACATTTAGCAGCATCCCTGTCTCTAGGATATGCAAGGTAAAGCGCGCTCGCTCTCTCTGAACACACGTGTATATAAACCCACGAGCAGGTTCCTGCCTGTGCCCGCTGGGAAGGCAGAAGAGTGAGGAATGTTGCCAAGCGAGCAGATGCACCCCGCAGAGCTGTGGCCCTCAGTCCTCTGCAGAGCGTGCGCTGTCCCTGTGCCACATGAGGACAGGGTACCTTCAGCTCTTGAGGTTTTCAGGTGTTTTGTTAGGTGCTGAAATCTACATTTTAAGAAAGCCTATCTTCTGGACTGGAGAGGTGGCTCAGCGGTTAAGAGCACTGACTGTCCTTCCAGAGGTCCTGAGTTCAGTTCCCAGCAACCACATGGGGGCTCACAACCATCTGTAATGGGATCCGATGCACTCTACTGGTGTGCATGAAGAGAGCAATGGTGTGCTCATACATATAATAAATAAATAAAAGCCAATCTTCTAGAAACCTGTCTAAATTCAAGGGCCATTCTGCCAATCCGGATCTTTAGAAAGCTAGGTGCTTTGGTCAGCCATATTGCTTGATTCTGGTGCTGCCTGCGCTGCCGGTCAGATGGTTGAAGGGTGGGGGTGGCTTGTTCTGCAGATCTTTGCTTCTGAAGTAGGATCTGGTTTCCTTAGACAAACACATGCTTTCTGACGAATCTGTCTGTGGTCATTTTCTTCATCTAGTCTCCAAATGAGCGCGCCCGCTTGTATTTCCTGCTGGCTTGGTTCCATGCCATCATCCAAGAGCGCCTGCGCTATGCTCCACTGGGGTGGTCAAAGAAGTACGAATTTGGAGAATCTGACCTTCGATCAGCTTGTGACACGGTGGACACGTGGCTGGACGACACAGCCAAGGCAAGTTAGGCTACATCACCCATCCCGTGATGGGGGCAGACAGTGGATCTGAACTCAAGGGAGGGCTGATACTTTGTAGCAGCCAGAACTAGACTGCAGCAGAAAGCTGGCCCCTCCAGGGTACACATGTAACCCAGCCTCTGTCCTCCCCAGGGCCGGCAGAACATCTCACCGGATAAGATCCCATGGTCTGCCCTGAAGACCCTGATGGCGCAGTCCATCTATGGTGGGCGGGTGGACAATGAGTTCGACCAGCGTCTGCTCAACACCTTCCTGGAGCGGCTGTTCACCACCCGCAGCTTCGACAGTGAATTCAAGCTGGCGTGCAAGGTTGACGGGCACAAGGACATCCAGATGCCTGATGGTATCAGGTGCGGCCGCATGTGGGAACAGAAGTCAGGCTTGGGGGAGGGGTGTTTGGGGGGAAAGCGTGTTTGGAGCCACAGTCTACCGGACTCTGATTCCAAATGTCCTGCTTTCTCAGGCGAGAGGAGTTTGTGCAGTGGGTGGAGCTGCTGCCTGACGCCCAGACACCCTCATGGCTGGGCCTCCCCAACAATGCTGAAAGGGTCCTGCTTACCACCCAAGGTGGGTAGTGAGTCCCCTCACTCCTGGACCCCTGCCCACCCTAAGCCTTGCTGGAAGGGCTTGTTCATGGGGAGTCCTGAAAAGAAGGTTTGAGGGCTAGCTTTAGTTCAGGAGTTGGAAGCACTGATGGAGGCTTGGTGGTATGCAGGCTGTGCCCTAGCCTGGAGCCAAAGGCAGAAAACCGTGGAGTTCAACATCCAGTCTACACAGCATGCCTCAAACAACACAGCATACCTTGTATTAAGTCAGTTTTACTTTTCACGGCATTGGTGGTAGAATCCAGAGCCTAACAAGCTAGGCAGAGCTCTGAGAGCAAGCCCCCACACAGTCAACAACCATTTAGAGCCAGGCTTTGTAACTCATCCCTGAGATGCCAGCACCCACGGGTAATAGGGTAGAGAGTTCTAGGCTAACCTGGATGACATGGGACACTTTTAAAAACAGCAGGTGAGGTGACTCAGCAGCAGCAACATAGCACCAGCCTGCTACACCCTGTCCTTGTCCTTCTGGTGGTCTGACCTGACCCTTATGGGAAGGCTAAGAAAACAGGCAGGGCTAGTGCAGGCATTCAGAAGTAGGTGAAGCAAGATGAAGTGTTCCCAGCTACCTCCTCCTATGGAGAACGCTCTGAGAGCTTGCCTGTTGTCTGGGAGGAGGTGACCATGAGAAGGTGGGGGTGTCCATGGACAGTAGGAAGTGAGCGGCTGTGACATGGATTTAGTGTGAAGATGAGTGTCTGGTCTGGAGGCTGAGCGGCTCCCTGTGGGGTTGGATGCAGTCTCTCCAGCACACAGGCTGATTGGTTCTCAGTGCTGGTGTCACTTGACCCAGCGCTTCCCTAAGCCCTGTACCCATCAACACTGTCAAGAACCAGGGCATTGCCCAGCTTCTCCACCTTTCTTTGCACAGTCCAGAGAGCAGGGAGCCTCAGCAGCCTGGGCATCAATTCCCAAGTGTGAGGGACAGGCCTAGCACCAGTGTGCTTTTAGTAACGTGGCATTTGTGTGAGCAGAGTTCAGAAGAGTCCCAGTGGCCATCACAAACCCCTAGCAGGGTCTGCCTTTAGGCTGAGTGAGGCTTCTGAGGCCTGCAGGCTACAGCCTCACAGCACACAACCAGGCCATTGGGTCTGGCTTTGTCTTAATTGCCCTGGGGTAGACGTGTGGCACGAGGACAGCCTACAGGCTGCCGTGGTGAGCTGATGGTCAAACAGCTCTTGGCCTGTCCACTGAAGTCCTCCCCACCCGGGCCTGGCCTAGGCGTGGACATGATCAGCAAGATGCTGAAGATGCAGATGCTGGAGGACGAGGATGACCTGGCCTACGCAGAGACGGAGAAGAAGGCAAGGACGGACTCCACCTCCGATGGCCGTCCAGCCTGGATGAGGACACTGCACACCACGGCGTCCAACTGGCTGCACCTCATCCCACAGACACTGAGCCCCCTGAAGCGCACCGTGGAGAACATCAAGGTAGCTGGGCTGGGCTCAGCGTGCTGCTCCCCAGGCAGGCAGATCTCTGAGTTCGAGGCTAGCTTGGTCTACAGAGTGAGTTCCAGGATAGCCAGGGCTATATAGAGAAACCCTGTCTCGAAAACCCAAAATACAAAAAAAAAAGAATGGCCACCGCTTTAATTAAACTCTTTGAAAGCTGTGATCTGAGAGGGGCAGAGAAATTCATCAGGCCAGGGCCACACTGCACTCTGGCCAGCTCCCAAAAAGGTCTGGTCAAAAGCTCTGGTGCTGCTCCCAGGTGTGCCTTGTCTCTGCACAGAGCCCGTCCTTAGCCCTCTTCTCTCTGGCTTTAGGATCCTTTGTTTAGGTTCTTTGAGAGAGAAGTAAAGATGGGGGCCAAGTTACTCCAAGATGTGCGCCAAGATCTTGCTGACGTCGTTCAAGTGTGCGAGGGGAAAAAGAAGCAGACAAACTACCTGCGCACGCTCATCAATGAGCTGGTGAAAGGTGCTGATGGGGTGTGCGGCTGGGAGCTGGTCTGCCACTGTCCACACCCCATGTGCGGGCACACTGCAGTCCCCAGGCCACACGTGTCTCTTTCAGGCTTCCTCAATAGTGGGGGTCTTAGCAACGCCCCTGGTTTTCCCTAGGTTTTCCACAGGCAGCTCCTGTGACTTTCAGGGTTAATGCAGTGAAGAGGACAGAGGGGTGGGCGGGGCCTGGTCCAGACACACTGCACGCTGCAACACCTCTAAGCAGGCACCCCACACTACTGAGACAGCTTTGCCCAGGGCCTTCCTGGTTGTGGACAGAACACCAGGCGCAGCCAGGAGAGCGGGCTGTGGTGTGCAGATGCTGCCAGCCTGAGCCACGTCTGCAGAGGGGGACTTGGCAGAACTAGTGCTGAGGCCTGCGGCTCTCTTCCTTGCTGCAGGGATCTTGCCTCGGAGCTGGTCCCATTACACAGTGCCTGCAGGCATGACTGTGATCCAGTGGGTGTCAGATTTCAGCGAGAGGATCAAACAGCTGCAGAACATCTCACAAGCAGCTGCATCTGGTGGTGCCAAGGAACTGAAGGTGGGCATGCATGGCCCGGGGTAGGGGGGTAGGGTCAGGAGTGGCACTGATTGTCCTGGACTTGGGTAGAAAGTGTTCTTTCTCGATGGCACTAAGGACAACCAGGGCCTGTACCTGACCTGCTCTCCTTTCCACCGCACTGTTTGCACAGAACATCCACGTGTGCCTGGGTGGCCTGTTTGTACCTGAGGCCTACATCACTGCCACCAGACAGTATGTGGCACAAGCTAACAGCTGGTCCCTGGAGGAACTCTGCCTGGAAGTCAATGTCACCGCCTCACAGAGTGCCACCCTTGATGCCTGCAGCTTTGGAGTCACAGGTGAGCTGGCGTCTGATCTCTCAGCCTGGCCTTTCTGTGAAACCTGTTTCCAAATGAATGTACCCAAAGTGTCTGGGCAGAGCCGCCCAGCTGAGCCTGTGGGTGTCCTGGAAGCCGAGCTGTGTGGCTCTGTATGGCTTCAGCCCCTTCTCCACATCTCCTCACAGGTCTGAAGCTCCAAGGGGCCACGTGCAGCAACAACAAGCTATCACTCTCCAATGCCATCTCCACAGTCCTCCCCCTCACACAGCTGCGCTGGGTGAAACAAACAAGCGCCGAGAAGAAGGCCAGCGTGGTAAGTGTCGTGGCCTCGCTCTTTGGCTCTGCCGTGCTGGGACTCTACTTGTGTGCAAACAACTGCACCCCAGCCCTGGACCCCCATGGGAGCTTGTCCCTAGAGCTGATGAACAGTAAAGTCCCTCTGTCTGTCTCACAGGTGACCTTACCTGTCTACCTGAACTTCACCCGGGCAGACCTCATCTTCACCGTGGACTTTGAAATTGCTACAAAGGAAGATCCTCGCAGCTTCTATGAGCGTGGAGTTGCCGTTCTGTGCACTGAGTAAAGCTAGACTTTTCTCCAGTCCTCTTTCTGGAATAGGAAACTCCCAATATTTAACCTTCATTCATCCTTAACGGTGTGGAAGGGTTGATGGACTGGAAAGCAAGTGGTTGGTTCGCGTTGGCGGGTGACAGGTGCTGCGGTTGGAGACGGTGGGATGGGAGAAGAGAAGAGTGCGCTGTCCGAGGGCTGACCGAATGGCTGGGTTTAGTAAATAAAACACTAAGCATGACGGGCTCCCGCCTCTTGCCGCTCTGCTCAGTCTGCTGTGGGAATCCTTGCGTCCCAGCCCACCTCACCTTTGTCACCGTGGTCACAGATGCAGAAGCAACAGGGTGAGACCAGGAAAGCTGCACCTCCTCCCTGAGGCCTGGCAGCAACTCTGTTCGTGACTGACACCCCTATCCCTGGGGACCTTCTCTCTCTCAGACCTAAGCCCTGCCACCAGTCCATCCTCCCACACTTCCTTTCCACATGACCTGTGGTGAGAGAGTGGCCTCGTCCCACAGGAAGGGTGACAATTAGCCTGGTCCTAGCAACTCAGGATCTCTAAATCCCTCTGACTGTGTGGTACAGGGTGGGCAGAGGCTGTTGTCGTAGCTGTTAAGGACTCCTTAGCACAGAGAGGCACTGTCCTAACTCCCTTCTCTGGGAGAAGACACAGCAGGTTCCTCTGCAGGAGGAAAAGCCTGTGTTCTCTTAAGGAACCTAGGGGTGGTGCAGGTGACACGGCACCCACGGCTTCTTGCTCCGTCCAAACACCTCAGAGGATATTGCCTGAGTCTGTGGGGATCGCTGAGACCACGAAGATCACCCCAGCCCAATAACTAGACTTCTGCTCCCAGAATACCAGCCTATCCCCAACCCCTCCTACAATCAGAGGGATCGTTCTGGACAGTGGCCACCAGCCCTGTTGTCCTCCCTGGCAGTGCCACCTGGGGCACTGATGAGTACCCATAACCAATCCTGGAACGGCCCCAGCAGCACCCAGAGGTGGACATGCCCTGCCAAAAACGGGCACCTCCTTGTGGTGCCGTTTTTATTTGCACTTGGGAACCTTGTCCCCACCGAGGGGAGGACAGGACCTGGAGAGCAGCCCCAGACGTGTGCTCAGCTTTGTTACCTACCAGAAAGAAAGTGAAAGGTCAGTAGACAAGCTGGCCGAAGCACAACTGGGGAGAAACAGCTCCCAGGAGAGCCCAGCACGACCAGAAGCCTGCTCCCAGGGGACACAGAGGGCTTGCCCAGCACGGGCAGTGAGAGAGCTCTGAACACACAAGGAACCGGAAGGGATCTGCCTTCGATCCAGCACTCAGGAGATGGGCAGGCAGAATGGCAGGAAATGGAACAAGATCCTGAGGTTTGGGGTAGGAGGCCCAGTGAAGGCCACTCGGGTCTATCCCTGAATTGCAATCTATTTTTAAATATTTTTTTACATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCGTGTGCACTCGCGCACATACCCGTACACATGCACACACACATACACCATTATGTGTTCCTGTGGACGTTGGTTCTCTCCT
GF3 -1 -1 1 GACTCTGCAAGCCTTACTAGTTCTG
GF4 -1 -1 1 GCCTCTGCCTCCTGAGTGCTAGGATTGAG
EX52 -1 -1 1 GAGAATTAAGAGGAAGAAGAAGTACGTGGC
EX32 -1 -1 1 CGACAGCCTGGTCCCCTTATCTCTGTCAC
GR3 -1 -1 1 GTGCTGCCTGCGCTGCCGGTCAGATGGTTG
GR4 -1 -1 1 GTGGACAATGAGTTCGACCAGCGTC
Comments:
Created
Target: 2803Ensembl ENSMUSG00000056508 -- 111906657 -- 111908491 ENSMUSE00000437587 -- ENSMUSE00000437583
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 7 oligo candidates from u/d regions
AOS Produced 13 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:12:1:121257530:1, start 111906656: end 111908491
Found 5 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
REJECT (2) gap pair G5:12:111913991:111914990_341 G3:12:111902157:111903156_308
ACCEPT (0) gap pair G5:12:111913991:111914990_341 G3:12:111902157:111903156_313
REJECT (2) gap pair G5:12:111913991:111914990_341 G3:12:111902157:111903156_303
REJECT (1) gap pair G5:12:111913991:111914990_149 G3:12:111902157:111903156_308
ACCEPT (0) gap pair G5:12:111913991:111914990_149 G3:12:111902157:111903156_313
REJECT (1) gap pair G5:12:111913991:111914990_149 G3:12:111902157:111903156_303
REJECT (1) gap pair G5:12:111913991:111914990_138 G3:12:111902157:111903156_308
ACCEPT (0) gap pair G5:12:111913991:111914990_138 G3:12:111902157:111903156_313
REJECT (1) gap pair G5:12:111913991:111914990_138 G3:12:111902157:111903156_303
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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