High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by dk3
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 184658
Chr: 6
Strand: -1
Status: Ordered
BACs: RP24-381B4 : RP24-536O2 : RP24-375A3 : RP24-242N5 : RP24-171K22 : RP24-401P13 : RP24-252O23 : RP24-483L23 : RP24-483P22 : RP24-502F3 :
Instance: Plate 141: Well D05: BACs RP24-242N5 RP24-375A3 RP24-536O2 RP24-381B4
Instance: Plate 203: Well C08: BACs RP24-242N5 RP24-375A3 RP24-536O2 RP24-381B4
Oligos on GRCm38:
Type Start End Length Seq
G5 113800149 113800198 50 ACCAGCTCTCTGTGCTTCCTTCTGGAGTCTGAAGAGTACTACAATTTAAA
U5 113794424 113794473 50 GCCACCTCTATACAGATGCCACCCTATAAAGAGGTAAGGTGGAATCTTTT
U3 113794181 113794230 50 TAGGGAACACAGGTACATCAAGAGCCACCTCTAGGACTGGAGAGATGGCT
D5 113793469 113793518 50 AGCTCTTGGTTTGTGTTATGCCTCTAAAGGTGTCTGAATTTGGGGGACTT
D3 113793347 113793396 50 TGTATAGATTTTTGGGCTTCTTTTTGAGAATAAGTCTGAAGGTCTGGCCA
G3 113790103 113790152 50 TGTCCATATTTAGCTTCATATGTGGGTCTCCTTTTTACTAGTCACTTGGA
Oligos:
Type Start End Length Seq
D5_D3 113743391 113743462 72 GCCCAGGTTGTATCCCCATAAAGCCACACTTTGCTGGGCCTGACTAATGAGGGTTCCTGTGTGGCCAGGCCT
G5 113750143 113750192 50 ACCAGCTCTCTGTGCTTCCTTCTGGAGTCTGAAGAGTACTACAATTTAAA
U5 113744418 113744467 50 GCCACCTCTATACAGATGCCACCCTATAAAGAGGTAAGGTGGAATCTTTT
U3 113744175 113744224 50 TAGGGAACACAGGTACATCAAGAGCCACCTCTAGGACTGGAGAGATGGCT
D5 113743463 113743512 50 AGCTCTTGGTTTGTGTTATGCCTCTAAAGGTGTCTGAATTTGGGGGACTT
D3 113743341 113743390 50 TGTATAGATTTTTGGGCTTCTTTTTGAGAATAAGTCTGAAGGTCTGGCCA
G3 113740097 113740146 50 TGTCCATATTTAGCTTCATATGTGGGTCTCCTTTTTACTAGTCACTTGGA
G5_U5 113744418 113750192 5775 GCCACCTCTATACAGATGCCACCCTATAAAGAGGTAAGGTGGAATCTTTTACTCATTCACTGAACAAGCACCTTTGAGCACCTGCAATATGGCAGGTGCATGGTGATTCCTTGTGTACAGATCTAGCAGTAACAGAGACAGTGTGTATGTGCCACCATTATAGACACGAATTGATACTCTGAGGAAAAATCCCAGGAAGTCGTGGCTGAAGATACGGCTGCTGTAGCTAGCATGACCAGGAGGTGCTATTTGAAAAGGCATCAGGCACGTGTGTTGTTCTCAGGGAGCGTGTCCCAGGTGAAGGGAACAGAGGCTCAGCTAGGGAAATGACTGGCTAGGGCAATGCTGGAGCTCAAGAGATACACTTTGTTCTTGGGATCCCTCCTGTCAGATCAGTGTGTCTATACCTGACCTGCCTCAGTTGCCCACAAAGATGTTCTTGGGACTGTGCCTACCAGCATTCTCTGATGGAGGAGGGGTGATAGTAAGGGCCTGTGGGTACAACCTCCCCAGATGCCCATGATCCCTTGGCTGATGTACAGGTCTCAAGGCCCAGGCAGCTCTGCATAGTGGGTGTGCTCTGTCATGTAGCTTGTGATCTAGGGGTTCCATGAGATGAAGCAAAGACTAGGAATTCTCCATAATTTTCAAAACTCTGGCTTTCCTCCATCTCTGATATGCATTTCTTACTTTGTAGACTCAGCTTGGAATTGCTCATCCATAAATCCTGGCCACCAGGGATTCAGCCCCAGAGGGAGGCAGTGGAAGGCAGGGAGCCCGGGATGCCTGTGAACTTAATCCCTGGCAGGGTGGATGGGGGACTGAGATCCTCAGAGGCCAAGATGTTGTCATCACTTAGTTTCTTGGCCCCTTAGTGCCCCATGCTACTGTGGAGTCACTTGCCTCTGACAGCAACAGCAGGGAAGAGGGAAACCCCTTGGCCCATAGCTCCCCACCCTAGCTAGCTCCAGCTGCGGTGCATCAGAGCCTGGCTCTATCCCTGCCTGACAGCCGAAGGGGCGGGAAGCCAAGCTCCCAGCAGCCTAGAGAGCTGTCACCAGGTTTAAATATAGACTGCTGAGCCTATAATTTGCAGCTGTCCCCTGCTGTGCTGTCTTTAATTAGAAATGGAGACAGCCACAGAGGCCTCGGTAGCCAGGTGGGAGGTCCTGCCTTTCATACACCCCCAGTGCAAGGGGAGGTCAAGGCTGTGCTTCAGGGCTGGGGCTCACCTTGCAGCTGGGCTTGGCTCCCTGGGCCAGAAGGCTGCCACAGCTCTCACCTGCAGCTAACATCTCAGTTGGGAAGCCTGGGTTGCTCTCTGGAGCTCAGATAAGTCCTTCTGCATGGGCTCCGGGCTCTGCCCCCAACCCTTTCAGTCCTCCTTCTTCACTATAGCTAGGGTGGGCATTATCAAGGCCAAATCCATTCTGGCTCTCTCTTGCTGTTACCTCTTTCATATTTCCGCCCTGTCCTTAGGATAAAGTCTTTGCTGTCTGGAGCTATAGGCGGTACAGGGTATGTCTGCCACTATAGAAGCCACAGGCTGAGTCCCCTGTCTACTCAGTCACCTTTAATTCTCAAAGCAACCTGCCTTCCTGTGACATAACCTTATCCCCATTTACAGAGGAGTAGGCAGGGACTCAGAGGTGGGGAAAGCTGTCATCGCAAATCCTCAGAGCTGTGCTGTGGCAGTCTGTTTGCAGAGTGGGGCTTCATATATTTAGAGCAGGGCAGGCCTCCAGGACCATTGGAATCAAGGTAGAGGGCATAAAGGGGCAGGGGTTGGAAGGGAGGTCCCTGCTGTGAACTCACCCGCCTTGCCTATCTGCACAGCCAGTTTGGTGAGCTTGCCCTGAAGCACCGACTTCTCTTTCTTGTGCATGTTGGCTTTCTTCTTGTCATCTGCATCGCCGCCCTCTGCACTCTTCAGAGGCTGCATCTCCATGGCAGCTGCCCCATCCTGCTGCTTGGCTGTAGGGGCAGAGCATGCATGTGCACACAGACACACACATGCAGGTTAGCCTAGAGGCCAGAACGCTTAGCACCCACCCTGCCTGCCCAGTAGAAATGCTGATTATCCTGTCTGGGCCCTGCATGGCTGGCAAAGAAGCCTCGGATAGTACCTGCCACTTCCAGCTGCAGTGTCTGGGTCCCAGAGGCTCCTGGGACCTGGCTCTACCCTAGATTCCCAGCAGGACAGGGCTGAACCAAGACCCCACCCTAAATGGGTCCTTGGCCCTCTCACAATTAGATAGAAAGAGAAGGACTTCCTTGGGTATTCTTGAGGCCAAGTTGTTGGGTTGTTAGGGAGTTAGCCATTGGAAGGACACAGAGCATGGCTTAAAGAGAAAGTAAGGTGTCCCATCTCCAGTCAAGGGATCCTTGTCATTGTGGGATGGACAGAAGGACCTGTGGCTCTTTGAGCAGAGGTCAGCCAGCCTTCCACCTATAACAGTCTCTAAGAGCCAACTTCCAGACCCCAGCTGATAGGTAGGCTTGGGAGATCCTGACCCTGGTGCATCCTCTACCTCACTGTGACACCTCTTTATCTTACCTAGTATTATGAGAATCATGGCTGCACCCCAAAAGATGCCAGAGCTCCCCACAGGCGTGGGTACGTCTGCCTCTGGCCCTGGTACATCCCTATCTCTGTGTGTGACTCTAGCCCCATGGCTGCAGTTACCTTTGCTCTGGCTGCTGTCGGCACTGCCATCCTGCATTTTACCTACACAACAGAGAATTTCAACAAACAACAGGCAGCACGGGTCATCACGAGGACGCAACAGACCACAGACACTCATGTCACAGACAGGAAGGGCTAAGGTGCTCAGGGATGCTGAGGAGATCTGGAATGAAGGCCAGCCTGGCCACCATGGTAGCCACAGGGGCTGTGGGGGCAGAAGGCTTTTTGTTCTCTTTGGGGTTGGTTGTGGGGGTGTTTCTGTTGGAATGACCTTGGCTTTCAGTGTTTTATAGATTTAAAGAATGAGCTGGAAAGTAAACGGAACCAGAGTCTACACACAGAAAGCAGAGGGAGGCTCCCACGTCCCCAAACTGAGCCTGCCACGATACTTAGGGCTGAAAGGAGGAAAAGAGAAATGTTCGAATCGTTCCTTCCTCCCTCTCTCCCTCTCATCCCCTTGCAGTAACGAGGACTTGAACCTATGGCTTTGCATATACTAGGCAGATACTCTACCACTGTGCTATAACCCTGGCCCTTTTATGTTTTATTTTTGAGACGAGGTCTTGCTAAGTGTCTTAGGCTGTCCTGGAAGCAACAATCCTCCTGGTTCACATTTTAGCTACATCACTTTTATTTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATCAATTTTCTAGAATGGAGAGGAATGGAGGTCAACATGACCCTTTGTCCTTTGTTTGGACTGGGTGGGGTAGACTATATTCTTCTGAAGGATATGATGCTAAAACACCAAGGACACCTCCTGCCTAAAAATCCTGCTGTAGAGTGTATGGGACATAGGGTGAGCAAGAGGTTTCCAGAAATGGTCACTGAAGGTCTGTGGGAATGTCCTGGTTCTAGGAGGAAGTCACTGGTCCCTGGAAAAGAGGTCTTAAGCAGTAGCTCCAGCTTCACAGACAGGCTAGCAAGAGCCACTGGCTAGACTGGGTGCTCCTGGTCATGCCAGGGCCTGATGCTGTCCGGTTGTTGGGAGAAGGGCTGGGTGTGGGGCTGTCTACCTGCCATCAGTGCTGCCTGGAACTCCTGCCCCAGCCTGGGCCGCTGTGGGATTGCATAGAGGCTGCAGCTTCTTGCCTGAGACACCTGCTTTCAGCTGTGCCTGCCTGTCCACTAGGCCTGTGTCATCCACTGAAATTTCCTATAGCTTTGGTCCCTCAGGAGGAGTGGGAGAAGGCTGTCTACCCATGACACCTACTGCCAAGGTCGCTAGGCTCTAGGTTCACATGGTCTCCATGCCTTTATGCAACACAGTGGTTCTCAACCTTTATAATACTGTAACCCTTTGTCTCCTCACTACTTCACCGCTGTACTTTCGCTACTGTTAGGAATCATAATACAAGTATCTGATATGCGACCCCTAAAGGGGGTCACATGACTGACCGGTCCAGAAATATGGCTGTAGTGTTTATCACTGTCTCTTCTGAGAAGGATGCTCCTGCCAGGCTCCCATATACAGAGGAGAAACTGAGGCTCTGAGAGGGAGGCAGTTTGCTTTGGAGTGTGAATGTTTTAATGCTGCCAAGACTCCTCTGAGCCCAGGGCAGGGGGCTCGTGGTGGCATTGTGAGGGGTTCCTGAGAATCTTGTCTCAACACCAACCATCTGTGTGGCTTTGCCTTTCCTAGTCCGTTCCCACCTCGGCACAGTGGGGTGTTGTATCTAAACTCTGGGAGCACCTGGGGTTTTACATTCATAACAGCCACGTCCAGAGTCCAGCCTTCAGCTGACTGAAGACATTTCTGCAAGTGTCATGTTTTGTCAGGCCCTGCAAGGTCAGGTGAGAGGAACTGTCAACGTTTTACTTTTATAGATGAGAAAGCTGAGGCCAATGGAAGTCCAGAGGCATCTTGTCTTCACAGAACACCTCTAAAATAGCCCTGGAGCCTTGAGATCAGCTTGCTACTGTCATTGTGGATGCTTGGTGGGTGACTCAGACAGACCCCGGCTCACTAATTGCTATCTATAACTCTCATTAGAGGGCCTCAAACCCACTTGGGGTTTCAGGATAAAGACCCCTTTGATCCTATTGTAAGATGCTTTCTTTTGGATTAAAAACAGCATGAAGAAAAGTTTGTTTTTATTTTTTTTTAAAGGGAAAAGGATAATGGGCAACAATTATTTTTGTTTCTTGTAATAATTTCACTAACTGGGTTAAGAGGTGCCAGAGGACTGGGGTCCCAGAAGCAGCTCTGGCATGCTAGAAGGGCTTGTTTTACTTCACATTACCAGATGTCCTAGCTGCTGCGTGTAGCCATTAAACCCAGCGATTCTCTCTTTTATTGCCATAGCGATTACTCGGCCAGACAAATAATCACCAATGCGCAGGCTGGAGGAAGGGAGCGCTTTGCTGCAGATGGCCTGTTTGTCAGAAGCAAGGGACCTATTTGAGGCTGTTATTAAACTGGTGCCCACCTGGCCGTGTATTGTACTATGGCAGCCAGCCCTCATTAGGGACCTGGAAGATGGGCCTGAGGATGAGTTAGAGAGAGAGGAACAAGGATGGCGGGGTGGGGGTGGGGCGAGTGAGGTCTCCCAGGCTCTGCTCTATGTATATTCTAGATGGACTCATCTTGAACCTAGAAGATCCCCCAGACAGATGACAATGAACAAGCTTCGCGGGGGGGGGGGGCAGGTTACAGGCTACGAGACCTTGAAAAGGTCACTTCTCTGTGAAATGGGCCCATGGCAACCAGAGGGATGCTGAGGAAAAACACCTGCTGAGCACTGTTACGGTTGACAGTGTGATGTGCAAGAGTGCAAAAGAAAATAATGACATAAAAACAGTGATTGTGATGTTTCCAGGATGGAAAAAGTGGAAAAGTCCCACTTTTCAGACGTGGGACTGAGGAGGGTCACAGTTAGGGATCCACTGCCACCATTTCTGTGGCTGTTACTTTTGTCATTCCTGTATCACCGAAGTTTGGCTCAGAGAAAGGAAGTGAGATTATAGTGGTTTCTCTCTCATCGCCCCAGGCCCAGCCACCAGCTCTCTGTGCTTCCTTCTGGAGTCTGAAGAGTACTACAATTTAAA
U3_D5 113743463 113744224 762 AGCTCTTGGTTTGTGTTATGCCTCTAAAGGTGTCTGAATTTGGGGGACTTGCCCACACGCTCTCTCCAAGTCTCTAAGTTGCAGTCACACATTTCGTAGCCCATCTACAAAGTACTAGCACCGCACCCTCCCTGGTTCCCTGACAGCCTCAGCTGTGGCTGCTTCAGGCACCCAACACTGCTCTTCTCTTCTCTTACCTTCACAGAATAGGCCAGTGAGATGGTGACAGCCAGAGGGAGGCCCTCGGGCACAGCGACCACCAGCACCGTCACACCAATGATGAAGAACTTGACAAAGTACTGTACGTAGACGGGTGTGCATTCCGTCAGCCATGGCTTCTTGTTGACCACGAAGGTGTCCACGGTGAAGTAGAGTACCAGGATGATCACTGTGATGGCCGACATCACCAGGCCTGTGACATGGGGACACCTGCTCAGAGGGCTCCCAGGTGGGAGGGGGGAGATGTTCACTAAACTGCTTCTGCAGGCCTCTTGGGCAGACCTCAGCTCAGCAGACCCAGCTGTTCTTCCTTGCTTTGGGGCTAGGGTCCTTTGCTTTGCTTCTCAGGGAGCCTCAGTTGCCTCTTCTGAACAATGAGGCATAGGGGGCAGATTTCCTTCCTGGGGTTTTGCAATGCTCACGTGGCAATGCAGGGAAGTACCTAGCATGGTGCCTGCCCACATGACACTCAGCCACACTAAAAACGTGTGGCTAGGGAACACAGGTACATCAAGAGCCACCTCTAGGACTGGAGAGATGGCT
D3_G3 113740097 113743390 3294 TGTCCATATTTAGCTTCATATGTGGGTCTCCTTTTTACTAGTCACTTGGACCATTTAAGTTTTGATAGATGAATTTATTAATTTGCATCCCACAAGTGACTCATTTTCTATTTTGGGAACAGGATCTCATGTAGCACAGGTTGACCTGGAACTAGCAACCAAGCCAAGGAAGACCTTGAACTCTTGATCCTCCTGTCTCTATCTCCTCAGTGCTGAGATTACAGATGTGCTCAACCACATCTGGATTCATCCGCATTCATAAAAGCAAAACTGTTTTAAAGAGATTTATTTAGTTTATGTATATGAATGTTTTACCTGCACCATGTATGTGCCTGATAGCCATAGAGGTCAGAAGAGGGCATCAGAACCTTGGGACTGGAGTTACAGATAATTGTCAACTACCATGTGGGGGCTGAAGCTGAACGTTCTGGGAGAGTGACAAGTGCTCCTAACCTCTGAGCCATCTCTCCAGCCTCCTCATCTACATTCATGTTGGAAAAAATAGTAGAAAATATTTAAAAGAATAAGGCTGGGCTATCCCTAATTACTCCTAGCTTACTGTAAGTAGTAGCCTCTTCTCATTTGGGTCTGAATTTCTCAAATGCTTTCCTGCTGCCTGCTGAGAAATCTACCCATTTCACCCTAAGTAGATACATTTAGGATACATACATTACAGATACAGGAAAATATCTGTAAAATAGGAAAATAATAATGTCATTGTCACTAGGTGATAGAGGAGAATCTCACTGACCTTGTCAAGTCTATAAATCAACTTTTCTGTAATGAACACTTACTATAGTGTGCATAATAAAACCATGTTTGTACGTGTTTGAGTGTGTGAGTGTGTGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTACATACGCGCATGGTGTCACTGGCATATGTGTGAGATGGTAACAGGAGCCTCCTCTGTGGCTCTCTGCTTTAACCACTTGAGACAGAGTCTCTCCCTGAACCTGGAGTTCTCTGCTTCTTTGGCTAGACTGACTGGCCAATAAGCTTCAGTCACCTTCCAGTCTGCCTGCTCTAAACGTGGGGGCAGGGTGGGGTACAGGCGCACGCAGCACACACAGCTGTTTACATGGGCACTGAGAGTCTGACCTCCGGTCGTCAGGCTTACACAGCAAACACTCTTACCCACAGAGCCATTGCTGCAACCCCAAGTACACAGGTGCACTGCCTAACCTACCAGCTCTGCCTAAACTTCCAGCTCGGCCTAACCTGGCAGTCTGGCTCATTCTACCCACCGGTTCACCAACCTGCCTTACTGAAGGAGCGTTGGCCCTTTAAGAATCTCTTTTTGGAAGCTAGGTGTGATGACACGTTCCAGTACTTGGGAGATAGAGGCAGAAGGATTGTTGTTAAGTTCAAGGCCAACCTGGGCTGCACAGGGAACCTCTATGACAAACAAAACAAAATCCCAAACAAAATGCCCAAAAATTCCTCACCAGGACTGTCCAGCATGGTAACTGCAGGGGGATGAGAGGCACTTAAAGTGTGGTCCAAAGTGTGTGAGATGATAGATTTTAAAGACATTATCAAACAGATAACCAAAGGTAACATTTCTCGTGTTGTTTATGCGTTGGATTGTTGATACCTTGGATGCAAGTTTGAGTAAATTTGTATGATTAAGATTAATGTTACCTGTTTTTGAGACAAGGGCTCATGTACTTCATGATCTTGAACTTGCTGTGTTCAAGATGACCTTGAACTTCTAATTTTCCTGCCCCATTTCACAAGTATTGGGATCACAGTTTGTGTATCTCCACGCCTGGTTTCTGTGGTGCTGGGGATCAAATCCAGAGCCACTTGCATACTAGGGTAGGCTCCTACCCCTGAGCTAGAATATCCCTAGTCACACTTTATATCCCACTCGTATATATGGATATACCTGCCCATATAGGTACAAGTGGAGGTTAGATATTGACATCAGGTGTCCTTTCTGCCTTGTTTTTCGAGACAGGGTCTCTCACTGAACCTAGAGCTTGCTGTGTCAGCTAGATCGGCTGGCCAGCTTTCAGGATCTGCCTGTGTGGGTCCCTCCTGAACTGGGGTCATAACACTGAGGTGACAGGTGTATGTGTCCAGGCCTGTCCTTCATGTGGGTGCTGGGATCTGAACTTGGGTTTTTACACTTACACAGCAAGCACTTTACACACAGAGACAGTTCTCCGGCCCTATTTTTAAAAAGTTAGTTACTGGGGCATTTAAAACGACATGTCTGGATTGGGTGACATCCCTGCTGAGAGGAACTGATGTACTTACACGCTCGCTACTCTGCTCATGTGGCTTTGCCAGTCACATGACCCCAGATGACCAAACCAGAGATAAAAACCTCAGAAGATTCTCAGGCTTAAAACTGGCCATTCATGATGTCCCCAAACCAGCTTTGCCAAACTTCACTGAGACCCTGAGTTCAGTGGTGTGGCAATGGGGATCGACTCATGGTTGTGTAAGAGTTATCCTGGCCGTGCAGGCCTGGAGAAACACTGTGTTATGACTTGTTCAGGCTTTGGTTTGTGGGAGGGGGGTTTCTTTGAGAACTGTTAAGAGAAGGAGCTGATCAGCACTGTATCCTTTACACGGCACTGCCCCTTGCTGGCCCCTGTGGGAGATCACTCTCTATATGAAATCACACCATAGGGTGCAGCCTAAATTCTGGCTGGGAAACTGACACTCGCCTTGCCCTCTGGCTGAGTTGCTGATGTCTCAGAGTGAAAGTCTTTTCAGTGATATCTTGGTGTTGAGACCATGAGAAAGAGGTGTCCTCATCCCTAGCCAAAAGGATGGGGACGCTCAAGGGGAGAGGAAGAACATGGACAGGTAGGACAATCTGGTGGTGGTACCCTGGGGGCAGATTTGTAGCACTTGGACTCTCTGATGGCCAGGGCTTCAGCAGGTACGTGGGTGCTTAGGGAAGGTCCTGGAGTCCCGCTTGACCAGCGAACATCCAGGTGGTATGCCATTTAAATCCTCCCGTGTTTTCAGCAAACCCTGCCTGACGGGTAGTGGCTTCCTGGGTGGTTTTGCTCCCGCATCCCACACGTTAGGGCATGACCTGTTGGGGTTTGGTGGGATGGAGTTGTGTTCTGCCTTGCACAGTGATGTCTACATTCGACAGTTTGCAGTACTGTGGCCTCGGGCTTCACTGAGCCGTACTCCGGTTCCATTTTATTTTGTAAGTCTGAGTTGTCAATACAGATCTAGAGAGTTTATATTTTGTATAGATTTTTGGGCTTCTTTTTGAGAATAAGTCTGAAGGTCTGGCCA
U5_15 113744418 113759468 15051 GCCACCTCTATACAGATGCCACCCTATAAAGAGGTAAGGTGGAATCTTTTACTCATTCACTGAACAAGCACCTTTGAGCACCTGCAATATGGCAGGTGCATGGTGATTCCTTGTGTACAGATCTAGCAGTAACAGAGACAGTGTGTATGTGCCACCATTATAGACACGAATTGATACTCTGAGGAAAAATCCCAGGAAGTCGTGGCTGAAGATACGGCTGCTGTAGCTAGCATGACCAGGAGGTGCTATTTGAAAAGGCATCAGGCACGTGTGTTGTTCTCAGGGAGCGTGTCCCAGGTGAAGGGAACAGAGGCTCAGCTAGGGAAATGACTGGCTAGGGCAATGCTGGAGCTCAAGAGATACACTTTGTTCTTGGGATCCCTCCTGTCAGATCAGTGTGTCTATACCTGACCTGCCTCAGTTGCCCACAAAGATGTTCTTGGGACTGTGCCTACCAGCATTCTCTGATGGAGGAGGGGTGATAGTAAGGGCCTGTGGGTACAACCTCCCCAGATGCCCATGATCCCTTGGCTGATGTACAGGTCTCAAGGCCCAGGCAGCTCTGCATAGTGGGTGTGCTCTGTCATGTAGCTTGTGATCTAGGGGTTCCATGAGATGAAGCAAAGACTAGGAATTCTCCATAATTTTCAAAACTCTGGCTTTCCTCCATCTCTGATATGCATTTCTTACTTTGTAGACTCAGCTTGGAATTGCTCATCCATAAATCCTGGCCACCAGGGATTCAGCCCCAGAGGGAGGCAGTGGAAGGCAGGGAGCCCGGGATGCCTGTGAACTTAATCCCTGGCAGGGTGGATGGGGGACTGAGATCCTCAGAGGCCAAGATGTTGTCATCACTTAGTTTCTTGGCCCCTTAGTGCCCCATGCTACTGTGGAGTCACTTGCCTCTGACAGCAACAGCAGGGAAGAGGGAAACCCCTTGGCCCATAGCTCCCCACCCTAGCTAGCTCCAGCTGCGGTGCATCAGAGCCTGGCTCTATCCCTGCCTGACAGCCGAAGGGGCGGGAAGCCAAGCTCCCAGCAGCCTAGAGAGCTGTCACCAGGTTTAAATATAGACTGCTGAGCCTATAATTTGCAGCTGTCCCCTGCTGTGCTGTCTTTAATTAGAAATGGAGACAGCCACAGAGGCCTCGGTAGCCAGGTGGGAGGTCCTGCCTTTCATACACCCCCAGTGCAAGGGGAGGTCAAGGCTGTGCTTCAGGGCTGGGGCTCACCTTGCAGCTGGGCTTGGCTCCCTGGGCCAGAAGGCTGCCACAGCTCTCACCTGCAGCTAACATCTCAGTTGGGAAGCCTGGGTTGCTCTCTGGAGCTCAGATAAGTCCTTCTGCATGGGCTCCGGGCTCTGCCCCCAACCCTTTCAGTCCTCCTTCTTCACTATAGCTAGGGTGGGCATTATCAAGGCCAAATCCATTCTGGCTCTCTCTTGCTGTTACCTCTTTCATATTTCCGCCCTGTCCTTAGGATAAAGTCTTTGCTGTCTGGAGCTATAGGCGGTACAGGGTATGTCTGCCACTATAGAAGCCACAGGCTGAGTCCCCTGTCTACTCAGTCACCTTTAATTCTCAAAGCAACCTGCCTTCCTGTGACATAACCTTATCCCCATTTACAGAGGAGTAGGCAGGGACTCAGAGGTGGGGAAAGCTGTCATCGCAAATCCTCAGAGCTGTGCTGTGGCAGTCTGTTTGCAGAGTGGGGCTTCATATATTTAGAGCAGGGCAGGCCTCCAGGACCATTGGAATCAAGGTAGAGGGCATAAAGGGGCAGGGGTTGGAAGGGAGGTCCCTGCTGTGAACTCACCCGCCTTGCCTATCTGCACAGCCAGTTTGGTGAGCTTGCCCTGAAGCACCGACTTCTCTTTCTTGTGCATGTTGGCTTTCTTCTTGTCATCTGCATCGCCGCCCTCTGCACTCTTCAGAGGCTGCATCTCCATGGCAGCTGCCCCATCCTGCTGCTTGGCTGTAGGGGCAGAGCATGCATGTGCACACAGACACACACATGCAGGTTAGCCTAGAGGCCAGAACGCTTAGCACCCACCCTGCCTGCCCAGTAGAAATGCTGATTATCCTGTCTGGGCCCTGCATGGCTGGCAAAGAAGCCTCGGATAGTACCTGCCACTTCCAGCTGCAGTGTCTGGGTCCCAGAGGCTCCTGGGACCTGGCTCTACCCTAGATTCCCAGCAGGACAGGGCTGAACCAAGACCCCACCCTAAATGGGTCCTTGGCCCTCTCACAATTAGATAGAAAGAGAAGGACTTCCTTGGGTATTCTTGAGGCCAAGTTGTTGGGTTGTTAGGGAGTTAGCCATTGGAAGGACACAGAGCATGGCTTAAAGAGAAAGTAAGGTGTCCCATCTCCAGTCAAGGGATCCTTGTCATTGTGGGATGGACAGAAGGACCTGTGGCTCTTTGAGCAGAGGTCAGCCAGCCTTCCACCTATAACAGTCTCTAAGAGCCAACTTCCAGACCCCAGCTGATAGGTAGGCTTGGGAGATCCTGACCCTGGTGCATCCTCTACCTCACTGTGACACCTCTTTATCTTACCTAGTATTATGAGAATCATGGCTGCACCCCAAAAGATGCCAGAGCTCCCCACAGGCGTGGGTACGTCTGCCTCTGGCCCTGGTACATCCCTATCTCTGTGTGTGACTCTAGCCCCATGGCTGCAGTTACCTTTGCTCTGGCTGCTGTCGGCACTGCCATCCTGCATTTTACCTACACAACAGAGAATTTCAACAAACAACAGGCAGCACGGGTCATCACGAGGACGCAACAGACCACAGACACTCATGTCACAGACAGGAAGGGCTAAGGTGCTCAGGGATGCTGAGGAGATCTGGAATGAAGGCCAGCCTGGCCACCATGGTAGCCACAGGGGCTGTGGGGGCAGAAGGCTTTTTGTTCTCTTTGGGGTTGGTTGTGGGGGTGTTTCTGTTGGAATGACCTTGGCTTTCAGTGTTTTATAGATTTAAAGAATGAGCTGGAAAGTAAACGGAACCAGAGTCTACACACAGAAAGCAGAGGGAGGCTCCCACGTCCCCAAACTGAGCCTGCCACGATACTTAGGGCTGAAAGGAGGAAAAGAGAAATGTTCGAATCGTTCCTTCCTCCCTCTCTCCCTCTCATCCCCTTGCAGTAACGAGGACTTGAACCTATGGCTTTGCATATACTAGGCAGATACTCTACCACTGTGCTATAACCCTGGCCCTTTTATGTTTTATTTTTGAGACGAGGTCTTGCTAAGTGTCTTAGGCTGTCCTGGAAGCAACAATCCTCCTGGTTCACATTTTAGCTACATCACTTTTATTTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATCAATTTTCTAGAATGGAGAGGAATGGAGGTCAACATGACCCTTTGTCCTTTGTTTGGACTGGGTGGGGTAGACTATATTCTTCTGAAGGATATGATGCTAAAACACCAAGGACACCTCCTGCCTAAAAATCCTGCTGTAGAGTGTATGGGACATAGGGTGAGCAAGAGGTTTCCAGAAATGGTCACTGAAGGTCTGTGGGAATGTCCTGGTTCTAGGAGGAAGTCACTGGTCCCTGGAAAAGAGGTCTTAAGCAGTAGCTCCAGCTTCACAGACAGGCTAGCAAGAGCCACTGGCTAGACTGGGTGCTCCTGGTCATGCCAGGGCCTGATGCTGTCCGGTTGTTGGGAGAAGGGCTGGGTGTGGGGCTGTCTACCTGCCATCAGTGCTGCCTGGAACTCCTGCCCCAGCCTGGGCCGCTGTGGGATTGCATAGAGGCTGCAGCTTCTTGCCTGAGACACCTGCTTTCAGCTGTGCCTGCCTGTCCACTAGGCCTGTGTCATCCACTGAAATTTCCTATAGCTTTGGTCCCTCAGGAGGAGTGGGAGAAGGCTGTCTACCCATGACACCTACTGCCAAGGTCGCTAGGCTCTAGGTTCACATGGTCTCCATGCCTTTATGCAACACAGTGGTTCTCAACCTTTATAATACTGTAACCCTTTGTCTCCTCACTACTTCACCGCTGTACTTTCGCTACTGTTAGGAATCATAATACAAGTATCTGATATGCGACCCCTAAAGGGGGTCACATGACTGACCGGTCCAGAAATATGGCTGTAGTGTTTATCACTGTCTCTTCTGAGAAGGATGCTCCTGCCAGGCTCCCATATACAGAGGAGAAACTGAGGCTCTGAGAGGGAGGCAGTTTGCTTTGGAGTGTGAATGTTTTAATGCTGCCAAGACTCCTCTGAGCCCAGGGCAGGGGGCTCGTGGTGGCATTGTGAGGGGTTCCTGAGAATCTTGTCTCAACACCAACCATCTGTGTGGCTTTGCCTTTCCTAGTCCGTTCCCACCTCGGCACAGTGGGGTGTTGTATCTAAACTCTGGGAGCACCTGGGGTTTTACATTCATAACAGCCACGTCCAGAGTCCAGCCTTCAGCTGACTGAAGACATTTCTGCAAGTGTCATGTTTTGTCAGGCCCTGCAAGGTCAGGTGAGAGGAACTGTCAACGTTTTACTTTTATAGATGAGAAAGCTGAGGCCAATGGAAGTCCAGAGGCATCTTGTCTTCACAGAACACCTCTAAAATAGCCCTGGAGCCTTGAGATCAGCTTGCTACTGTCATTGTGGATGCTTGGTGGGTGACTCAGACAGACCCCGGCTCACTAATTGCTATCTATAACTCTCATTAGAGGGCCTCAAACCCACTTGGGGTTTCAGGATAAAGACCCCTTTGATCCTATTGTAAGATGCTTTCTTTTGGATTAAAAACAGCATGAAGAAAAGTTTGTTTTTATTTTTTTTTAAAGGGAAAAGGATAATGGGCAACAATTATTTTTGTTTCTTGTAATAATTTCACTAACTGGGTTAAGAGGTGCCAGAGGACTGGGGTCCCAGAAGCAGCTCTGGCATGCTAGAAGGGCTTGTTTTACTTCACATTACCAGATGTCCTAGCTGCTGCGTGTAGCCATTAAACCCAGCGATTCTCTCTTTTATTGCCATAGCGATTACTCGGCCAGACAAATAATCACCAATGCGCAGGCTGGAGGAAGGGAGCGCTTTGCTGCAGATGGCCTGTTTGTCAGAAGCAAGGGACCTATTTGAGGCTGTTATTAAACTGGTGCCCACCTGGCCGTGTATTGTACTATGGCAGCCAGCCCTCATTAGGGACCTGGAAGATGGGCCTGAGGATGAGTTAGAGAGAGAGGAACAAGGATGGCGGGGTGGGGGTGGGGCGAGTGAGGTCTCCCAGGCTCTGCTCTATGTATATTCTAGATGGACTCATCTTGAACCTAGAAGATCCCCCAGACAGATGACAATGAACAAGCTTCGCGGGGGGGGGGGGCAGGTTACAGGCTACGAGACCTTGAAAAGGTCACTTCTCTGTGAAATGGGCCCATGGCAACCAGAGGGATGCTGAGGAAAAACACCTGCTGAGCACTGTTACGGTTGACAGTGTGATGTGCAAGAGTGCAAAAGAAAATAATGACATAAAAACAGTGATTGTGATGTTTCCAGGATGGAAAAAGTGGAAAAGTCCCACTTTTCAGACGTGGGACTGAGGAGGGTCACAGTTAGGGATCCACTGCCACCATTTCTGTGGCTGTTACTTTTGTCATTCCTGTATCACCGAAGTTTGGCTCAGAGAAAGGAAGTGAGATTATAGTGGTTTCTCTCTCATCGCCCCAGGCCCAGCCACCAGCTCTCTGTGCTTCCTTCTGGAGTCTGAAGAGTACTACAATTTAAAGTCTCAATTGTTGGCCTGGATTGGGGTCTGATGGCCTGCCTCTCTCCCTCTCTCCCTCCCTTTCTCCCTTCCTCCCTCCTTCCTTCCCTCTCTCCCTCCCTCCCTCCCTCCCTCCCTTCCTCCCTCCTTTCCTGCCTTGCCTGCCTTCCTTTTTTCTTTCTTTCTTTCCTTCTTTCTCAGGCTCCTCTGATCAGTCTTCATACAGAGAATATTATGGGCCCTATCTATGCTAAGCAAATGCCTGGCCACTGAGCTAGGTTCCCAGCCATTGAGATCTTTCACACAGGACCCTACTCTATAGCCCAGGCTGGCCTCAAAATTTATGATGTTCCCATCTGCTGGGACGCTTGTCTCCTAAGTGTTGGAATTATAGATTCATATCACCACATCCGGCCCTCAAATGCCTTTGATTAGAGGACCATCTTCCTGGGGGTGGGTGGAACTCTCTTCATTTCCTTGGAAAGCCTGTAAGACCCACTTCATTGTGGAATCCTGGGTTGCCTGGAGGAAGACATCTCAGAGCCAAGCACGTCTATGTTCTGGATTTACAGGCCCATCATGCTACTTTATTTATTTAATTTTATTTTTTGTGTGTGAGTGTTTTGTCTGCATGTATGTCTGTGTACCACAAGTGTGCCTGGTGCCTGCGGAGGTCAGAAGAGAGTGTTGGACCCTCCATGCTTGTGTGTGGGGAGCACAGGCAGTCTTGAGCAGCATGTGGTGAGAAGCTAGAGCTCTTATGTTGTTGTTTTGTTTGGAGATGGGAGTCTTTGGAGCCCTGGTTGTTCTGGAACTCACTATGTAGATCAGGCTGGCCTTGGGCTCACAGAGCTCCACCTACTTCTGCCTCCTGAGTGCTGGGATTAAAAGAGTGTGCCATCATGCTGGGATTAGACTAGTGTCCTAATTTCCTTGAGTCTCAGTCTCTTCATCCTTCATGTTCAGACAAAGCCTGTGTCACAGGGCCTTCAGGAGGATGAGAGTCAGAGAACGGGAAACAATAGTAGCAGCGTCACTTGGGAAAGCAGAGCTCCTTGTCCTCTCTGTGGGTCCTGGCTGCAGCCTGCAAGGGAAACTCTCCTTTCCCTCTGGAGCTGGCTCTTGGGGATGATGTCTGTATACTTGTCTCCTAGGGAAAGTGATTTCTGGGTTCCTTCTAACTCAGCTGTGGGTTTGCCCAGGCAGGCTCTTTCTGTCACCTCTCAGCCACCCCTCCCTGTTGCCTTCCCACCCACAGCCCCAGCTGCCTTCCTTCCTCCTCCTGGGCTTGAAATAGACCCACACTAGAATTAGCAACTGCTTGCAGAGGTGCCTTGTGCAGTCCCTGACATCTTCTGGGTGGGTCTCTTGGTTGCTTTGAAGGCAGGTAGGTTAGCTAGAGGTAGGGTATAGGGTAGGAGGATGAGATGGCTTAGGGGATCCACATCCTGCAGGAGGGGTAGGAAGTGAACTACAGAGTGGATAAGAAACTTGTCTAGCACTCAGCAATGGACACTTCTGACTCTTGCCTTGGCTTCTCTGGTAGTATATTCATAGCCAAACCCTAATGTCTTGAGCATCCAGGTATGGCCTAGGTGGTTATTACAGGACCTAATGGTGGTGGTATCTGACGATGGTGGACTGCAGAGATTCCCCAGAGCAAGTGGTTAAGGTTCTCTATGTTTGTGTAAGGAGAGAGTGATGAAGACCCAGGGACATGGGCCACCCCAACTAGCCACCCCCGGGAAGTAAGAATGCAGAGACCCTTAGACACTCCAGCTCAGGCACCAGCAAGACACACCAGTCCAGACTAGAAGGACAGACTAAGCCCAAGTCCCACAAGCCTCGATACCTGCTGGCTTGGAAAGAGAGAAGAGAAAGAGGCTGAGGAAGAAATGTCTGTGGCTCAGCCATGCTAGGTTAAAAGGCTGAACTCCAGCCTGAGTCTCTGCCTTTGGGGAGTTTTAGAGGAGGCTTGGAGCTTTTTTCTATGAACCCAGATGCTGGCTTCTGGTTAGTTCCTGACCCAAGGTAATGTTCTAAAACCAGAGCTAGAACTATGCTTAAGGGGGGGGGGCACACTGGGTGGCATACTGCTTCTCATGGGGCCAGAAGCCAAGGTCATGTGGAGTTGACTTCCTGGGTTGTGGTTCGGCTTTTAAGTACCTGTCCCATTTGGGAGGGAGAGTGTTCTGGAAGGCCATGCATGTCAGAGGTCTGTGGGACCCAGGATAGGGAAAGCCTCAAGTAGGGGGTAGAGAAGAAGACACCCAGCAACCCAGCCCAAGAGCTCGCCGTGGAGACGTACCATTGACTAGGCTGGCATTTGCGCTACCTGCAGCGTTTGCAGGCGCTGCGCCGTCGGCCGCTGTGGGGGTGGGAAGGATGGAAAATGCAATGTCACAATGGAGAAGGCGACCACAGAGACATGAGCCTACTGGGGACAATCACATGTCTTATTCTTAAAAACAAACCAAACCAAAACAAACCACTCTGCTGTTAAGTGAGGAACTCAAATTGGGGATGGGGGTGAAATGTTCCTGTGGATGTCATAGGGGAGGGTGGGGACTGAGACTCAGGGCAGCATGGAGTGTGGTCAGATGCTCAGGTGGGATGGCACTTTAGTAGTTATTTATTTTGAGACAGGGTCTCCCTGTGTAGCCCTGGCTGGCCTAGAGCTGGCTCTGTAGACCAGGTTGGCCTTGAACTCACAGAGACCCACCTGTCTGCCTCCCAAGTGCTGGGATTAAAGGCACACTCCACACAACTGGTTGGGATGGTACTTCTGTGGTGACATCTGGACTGTGTTGGCTTGGCTGGTCAGGTGTGGACCCCACGGGATCAGGAAGGCCAGTCCAGGGACTCGTCAATTCAAAATGCTCAGTGAGTGGTATATGGCCTGGACAGTTCTAGAGGGCATTCCCTGTGACCACCACAGTTGTGTCCCTGAAGAACTGCTGTCAGTGGATGGTGATTTAAGCTCAGGCCATTGCCCACCTCCTTCCCTGACCGGCTCGCTGAAGATCACTGTAGAAACCCCCATGTGTGATGTCTCACTGGAAAGGGGTATCTCATGTCTCCTTTTATATCAAGGAGATCTCAACTTAGAAATTAACAATGGTGTGACACCACCAAGTTCACCTTTATGGAATTCTTTCTTGGGGTGGGAATATGGTTATGTGTGAAGCAAGAGGAAGGGACACTAGTGAGTCAGGTGGACACACTGTGTGTTTGGAGACAGGAGCTGAGACTGTTTAGTAGGGACGTTGGTTGTGGGGCAGGGGTGCTATGGTGATAAGAGAGGTACTGCATGCTAGTGTGTGGGGCTTCAACGGAGGTGCCATTTTTGGCCTGGACAAGGGGGAAGAGTGAGCATTTTATTTACTTATTTATTTCTGGTCTGATGGGCTGGAATACAAGATATCACAGAGGAATGTCTGGAGGGGATAATCTCTAGTATGCAGGGGTCTACTCCAACTACGTGCTGCTGATCTACGGGCAAGGCCTAGGGGCCCACAGCTGCAGCTATTCCTACGAGCCCATCTTCCCTGAGGGTTGTCTTACTGTACCTGGTATCTGAAGGCCATCCCCCTTCTTCACACCTGTGTGATGATTTTTGAGACATGTTAATGAAGGAGAAGCACACACAACCTTGTATTCATGCCACATCAGACCACCACATACACACACGCACACACGCTCGCGCGCGCACACACACACACACACCATTGCATGCACACATGCACATACACACACACACCCCATGCATGCACACACACACATACACACACATGCATGTGCACACACACACAAATACATACATGCACACACATACACTCCTGCACATGAGACCATTCTGGACTCTGAGCTGTGTTTCAGGTTGACATGATGGGACGAGAAGTCCAAGGGGTCCAATTCTGGCTCCTCTGCCACAAGCCATTTATATTTCACCTTGACTCCCTACACAAAACTGAATTTTGTACCTCTGTATTCTGGTTCCGTTTTGGCAAAGGACCCTGAATGGTACCCAAGAGGTCACTGATGCCTGTCTTCCTCATGACATTGTTAGATATAGAATGCATCTGCAAGAGCTTCTGGGTGTCTCTCCCCAGCCAGGCACCACAGATATTGGAACATGGGTTCAAACTATTGCATTCCACTAGATTCTTAGCTTTGTCCTATTGCCTGCACTTAGTCTATTCACCCTGGCTACTGGTCCTTGTTCACTCATGAAATCCTGTCCCAATACCTGCTGGGACTCTCAGTCAAGACTTTGCCCCATTTTGAACTCAGCCCCACTTTGCTCTTTCCAGCATCTAGACTTCCCAGCTAACAGTGAAGCGTCACTGTTAATGCTCAGTATTGAAGGGACAGCTGTGACCCTAGGGGGACACACTAGAGAGGAGTTCTTTCCTTAGGTACCAACAGCCTTTAGCCGGGATGCATGTTTTGTATTCCAGTGGCCTCTGAGCATGGGTAGCATACCTGCTTGAACTTGGGGAGGGCCATCTACCCTGTTATCAACCTTTCCCAAGCTCCCAAAAGGTCAGAGTGTTGCGGAAGCTTCCCTGGAGCTTAGGAAAGGAGTCAAAGGACTGACCGTCTTAGATGGCACTGTGCCTAAGACTGAGTGTGGACTACAGGTAAGGAGTTGGCTATTCTCGCCTTTGGGTTCAGGACTTTTTTGTGTGAGCATCTCTCTTCTATTTTGAAAGGTATTTGTATCATAGACTGCTACTCACCGCACTGAAGGACACCAGGTAATAAGGACTTTTACTTTTAAAGGGTAAATGGAACCAGGACTTGGGCATGGTGAGCTCCTGGGAACTAATTTCAACAACCTTCCCAAGAGTCACCTCTGCCCATGACAGTCACTGTTCTGTGTAGGATAGATTGAAAACTCTGCTGGTAGAATTAATAATGTCAGAAGGAACATTTCTGTGTGAGGACTTCTATTTAATAGTGAGGTGATGGGGAACCAGCTACTTCTGGCTGCCTCTACTGCTGGAGCCTGGAGATTGAATGACATGCCCAGCATCCTGAGCCCGACCTTAAGGGAAAGGAAGGGGCTCTGTGACATGACTGGACAAGACAAAGACTAGGGAAGATGGAACATATAGAACCCACCCAACTTCAGCAGACCCTGAGGACAAAGGTGGCAACTATTGGCACAGGGCATGATGTAGCGAGTGCTTTATAATGTGGAGTGAACAGATGAATGGAAAATGTGGCCTTAAAAGCTCCTTTCCTGGTTTTGCAAATGTGTGTGTGTAGGAGGCTTACCTTTTTTGTCTTTCTTCTCTTCCTCTTCACCACCAGCCCCAAGCAGGGTAAATATGATGCCAGTCTGAGAGTTCACACCCACAGCAGTAACCACCATCCGTCCTGAGCCCTCCATCACATGGGTTCCTGAGGAAGGTATAACCCGGTTACTATCACAGGGCCAAAGATTCCTCCACAAATGAACAGAAGGAACAGGTGTCTATCAGCTTGTAGTACCTTGGTCACTGAGATAATGAGTGGCACTTTTAGTTAGAGATGGGCTTGAAACAGTATTACAAGATTGCTGTTGTCTCTCAGTTCTCTGTTCTATAGAAAACACAGGCCAGAACCTCCTCTCCTGGCTTTAATAGCCACTTCCTGCTTGTCTCTACCTCCCCTCCCCAACTTGTTCTCCCACCATTATTATAGGCCAAGCAGGTAACACTCTCTTCTTATGAATAGTATTTAGAATCAGACCTCATTCTGCAAAACTAAGAATAAACGGGAACTAAGGCTTAGAGAGTACTGCTGAGGACTGGCTCACCTTCCCTTCTAATGGAGAATTCAGGGGTCTGTGAACTGAGATGAGAAAACCTCAAATCATGTAAGCTTTCATAAACCTCAAAGTGAAATTCAGCGTTTTAGCCCCATTATAAATATACATAACAAATGGAACCTATCACTAATAGCAGCACACATAATTGTAGATACATTCTAGACTCCTGGTTCTTTTCTTATGATTTACAGCTGTGGTGGATGTTGACTATCATCTCTGACAGTGGACCAGTAGTTTGCCTTCCCACTGGATCTAGTTTTGTGTGCTAATATATAAACACAGACATGACTGTGCTGCACACTTTAAAACTACTTTATTTCAACATAACTGGTTTCTGTAAGTCATCCTGTGCAAACCTTCCAAGTTTTATGCTGAGGAAGTGGGTATACTGTGACATCAGATTCCCTGGGGGCATTTATGGGGCAAGAAAATTCCTGGCACTTTTCCTAGAAAGGTTCCACTACTATTATCGTTGTTGTCATGGTTACTAGTTGCGGTACTAGGGATTAAATCTAGGCCGTGTGCATTCAAGGCTAATGCTCTACTGCAGAGTGCCTTCCTCAGTCACTTTTGAGACGGGTCTCATGTATCTCCGCTTGGCCTTGAACTTGCTGTGTGTGCTGCTGAGGATGATCTTACACTAAAGATCCCCATGCTGCAGCCTTTCCAGTGCTGGGTGACAGGTATATTCCACCATGCCTGATTTATGTGGTGCTAGGGATGGAACCAGTGTATGCCAGGCAAGCACCCTACCAGCCGAGCCATATCCTACTTAGCCTTTTGGCATATTTGAGTTTCCAGATACCATCTCACTAAGGTGCCCAAGCTTGGCCTTGACCCCACTCTGGCACAGGCAAGTTTTGAAACTGTGATTTCCCTGCCTTAGCCACTTGAGTAGCTGTGACTGCAGGCCTGCATTACCACATTCAGATCATTTGTTTTAGAATCTCAAGTTTCTCAAAACGGGTTCCAGAAAGCCCTAGAATTCTTAGGGGTCTGGAAGGGCAGCTACCGAGTGAAGTGGCTCTAGGTTTACTCATCTTCATAATTTATTTGTTTGTTTGTTTGTTTATTTATTTATTTATGCTTTCCTAGATGTATCTCTGTACCACATGTGTGCAGTACCAAAGGGAGCCAGAAGAAAACACTGGATTTCATGGAACTGGAGTTACAGAAAGTTGTGAGTTGCCTCATGGGTACTAGGAACTGAACCTGGGTCCTTTGGCAAAGCAGCAAGTGGTCTTAACTGCTGAGCCATCTCTCCAAGTCCCATCTTTGGGGCTTTTGAATCACCAAGTCTTGTTTTTTGGAAGGCAGGCCTCTACTGTGCGGGCTCAGAGAGTGGCTGTCTGACACCACAGAGAATTTGTGGGAGAGGGAGACAAATGATATGTTTGTTATCCGTGTGTTTGGATGGTAGAAGAGGTGGGGGGATCAGGAGTTCAAGAACATTCTCAGTTAAAGGGCAGCCTAGGGTACATATTGAGTTTGAGGGAAGCCTGGGCTACACGAGACCCTGTCTTAAGAAACAGTAAGACAAAGCAAAGGTGGGGAAAGGTGCTCTCTCTGCCTCTCTTTGAACACTGGACGGTCAGTCTTCAGGAGATGGCAGTGCTGATACATGCTGAGACACGACGAACCCTAGGGACACTAAACAAGCTGGAACCTGAAAGCCATAAGGTCCACAAGCTTCTGAATCCAGAGACAGAAAGCAGAGGGGGGTTGCCAGGGGCTGCCGGAGACTGCCGGGGAGTGCCGGGGACTGCTGGGGACTGCCGGGGAGTGCCCAAGAGGCTGGAGTTTCAGAGATCAGGCTGAGCTTTGTCTGGGAAACATGAGCATCTCTGGAGGTGGAGGGAACCTGTGTTACAGGAAGGTGCTAAGCATCACTGAATCCAACATTGGCTGCCTGGGAAGCAGACAGCAGCCATGCTTACTCAGTCTATCCACAAGGAAGTGCAGGGTGGCCTTGCCCCTCAGCCAGCTCCCGCACATCCAGCAACTATGTCTGCTCTCTGAGACTATCCAGCAGGGTAAAGCTGGCCTACACTCACAGTGTTCTGGGCGCTAACTCTGTTCATATGCTGGGAGGAGGGGATGACTGGCTCATGAGACATTTAAAGAAACTGAAGCCCCAGAAAAGCATTGGAACACCTATGTACACAAAGGAAATACTTGGGACACCTACTGAGTCCTGGAACCATGCCACACCTCCATCCTATAAGCAGTAATCTCAGCTCTCACACCAGGGAGAAACTGAGGCCGAAAGGGTAAGGTAGGTTGCCCCAGACCCCACACTGGTGAAAAGCTACGGCGAGCTTTAAAACTCTGTCTTTATAGCCTAGAATCTTGAACATAGTCCACTTCTCCCAAAGGGAAAGGCCCCAAAAGCTAGCCCTAAAATCTGGGTAAGAGGAGGCTGTCCTGGAGACTGCTGTGCTGGTCCTTGGATCCAGAGGCCACAGGAAGGCTTTTTCCTTTGCCTAGCAGAGGAGGGATGGAAAAAGATGTGGGGGCCAATTTTCACCCATGATAAGCAGGCCTCAAATCACCTTCTAATCTAATCACTCCCATCACCCCCTGGGGGCTGGCAGCTCCTGTAACCTGTGTAGGTAGAGAGGGGACAGAGAAGGGGGCAGGACATAGGGCTCTACAGATCTGGGAAACCCCTGAGGGCTTCTCAGGCTACCACTGGATTGAAGCAGGGGCTTGGGGGTTGGGGGGAGCACAGCTTCTCTCCCCCAAATGTCACTCTGACAATGTCCCACAGCTCAGATCCTTCCAAGGCAATGGTTCTCACCTGATGCTGGGACCCCCAACCATAAAATTATTTCCTTGATACTTTATAACTGTCATTTTGCCACTGTTATGAATCATAATGCAAATATCTGACATGCAGGACCCTGGGAAAGGGTCATTTGACTTCCAAAAGGGTCATGACCCACAGGTTGAGAACTACTGTCCTAGGGGCATGGCTGGCTCTCTGAGGACCCAGTGGTGCAGTCAGACGCAGGATCAGATCTCAGACTTCGTGTGTTCCTTGTCTCCTGACCTCTGGCACTTGACTGCTGACCCTGGGGCATGCGAACCCTTTTTCAGAAGTTTGACATAGCCTTAAGGTTAAGTAAACTAACCCAAGCTAATGTTCATATTCCAGAGTCTATCAGATGTCACCTGCCTCTCAGGGACCTGCTGCAGGGGGATTGCTGGGTAATCGCTATGGCTGGTGCCTCTCTAGGCTATCAATAGAACAACTGCAGCCTTGTTCTGGTATCTGGGACCCGTGGTGCCACAGACCTGGGCTTTTGGGATTTCTTAGCCCTTCTCTCTTCCCATTCTGATGTACCATGGTTTGGTGTTTGTCCATCTATCCATCCTGAGGTCAGTTATGTGGATGCTTATAACCTGGTCTAATGCTTCTGAAAATGAGATCTTTCCAGACTCTTCTCCTTGTTCCAGGGATGAACTTGGGGTTTGGT
D3_15 113728342 113743390 15049 AAACAATCCAGTGTTGCAAATGGCAGAGCTGCCTCCTCCGTAATTCAATTTGTTTCTCTGAACTTGAGCTCTGAAATCGCCTACGTGCCTTATTAAGAAGCAGAGGCCCTCCCCTCTTTGCTCCTGACTTTGTTCTTCCTGACACACTCTGAGGTTTTCGCTCCTGCGTTCCAGCTGCAGGAGGTGTCTGCATTATGGGGCTCCTGCTCTGTGGCAGGAATGGAACTGCTCAGAAGGAGGGAGGGGCCTCGGCATCCCTGCCCCCTCCCCATAAGCTGCTCTTGCTACACACCTGCTGAAGCCAGGTCTGGGCTGCTGCAAGAATTTCGAATCAGTTCTGGAGTCCCCATGCCTGCTTGGGTGTTAAGCCCCTGTGCTGTGCAGGGTGAGGCTGTGGGCCTATCATAGATGGACCAGCCAATGCTTCTGTCTTGTGATGCTGGGGAGTGAATTCAGTCAAAATCAATACAAGATGATTTGAAGAGCTACAAACAATAAATACAGTTAGGGAAAAAAAAATGGGACTAAGGGCTTGGTGTGAGATGGCTCAGCTGGTAAAGAGGTGCCTGCTGCCAAGTCTGAAGACCTGAGTTCAAGCCCCTGAAGCCACATGGTTGAAGGAAAGAGCTGACTCCACAGTGCTGCCCTCTGACATCAAGTACGCTGTGGCATCTGCACTCACACACAGCACACACCCATGCCCATGAATACGTAAAATTAAAACCAAATACCCAGTGTGCATCACCTCATACCCATTATGATGCTACTATTAAGTAACAGAAGTTTCAAGAAATAACACATGTTGGCAAGAATGTGAAGTGCCCGGAACCCTTGTACAGCATTAGTAGGAACACGAGACGATGCAGATACTGCAGAGAGCCGGAAGCCCCTGAAACACTTGATCTAGAGAAACTGTGTCACACAGCAAAGTGTACTTCTGGACATCTTCTTAAAACAGTGGCTGCCAGGAACTCAAAGAGACCTTAGTGCATTCACATCCATGGCGGCAAAGTTGTGGAAGCAACGGGCAGCTGAGTGGAAACACAAACTGTGGCTTACACACATTATGGAATAATGCCTGCCTTAAATAGGAAAGACCCCCTGACATCTGCTATTACACGGGTGAAGCTGAGGACACAATGATAAGAGAAATAAGCCACACAAAGGTCTGATCTTAAGAGGTATCAACTCTTAGAGATGGAGGGTTGAGCGACTGCCCAGAACTCAAAGAGGGGGTGACAGAAGTTGGTGTTAATTAGCCAGAGGTTCAGTTTTGCAAGACAACAAGTGTATGGAAGGGTCACAGCCATGGGAATGTGGGTAATCCTACTAGCACATGTGTTTAAAAGATCGAAAGGCCAAGATGGCTAAAATGGGCAGATCTCATGGTATCCGTGCTTTATTACAATGAAAGAAAATACCCAAGCAAACTCCATGGTAGTCGATACTTTGAACATTAAATACCCTGTAAGAGTCAAAACATCTTAGAGACTGACACTGTTAGGGGGTGGGAGAGTAGGTGTTTTAGAGTCACTGAGTGTCTCCCAAAGGGGGACATTCTAGGACGCCAGTCCCCTCGTCCTGATCGCTTTGGCTTCCTGGCACCATGGTGACTGGCTCTCTCTGCCACAAGTATATCTCCCCCACGATATGTGGTCTCACCACAGGCCCAAAGCAAAGGCCCCACTGACCACAGACGGAAACCAACCAAAATGACCTATCCCTTGTTCGAAGCTGGTTTCTCTTGAGCATCTGTTACATAGACACCAAGGTTGATAAAGTGTAATTTGGTTATTGTCCACATGACTGGCTTCCTGGGACCAAAGCCTCATCCTGAAGGACAGACAGTGGCAATGAAAGCCCCTCCCTTGCCATGTCTGGATTAGTCACCAGATGTGGCCACTTCACTCCAAGTTGCCTCTGTTGCCTGCTGAGCCAGTTGCTCACAGACAGGCCCCTCAGGTGGCAACCTCATGGTTCCCAGGAGGTGGGGCTGCTATGTCCATGTTAAAGCTGTGGGCTCTGAGGCCCCTGGAGGAGAAGTAGAATTGCTCACCTGCAGTTGAGCCAGGACCTGTCCCTGATTCTGCACTCATAACAACTGTTGGTGGCTCCTGTCTAACCTCTGGAATGCTGTCACTGAAAGTGACAGGGATTGCACACTGGATTCACTTCTGCCTGCTTCCAGCACCATGGGCCAGGCAGAGACCAACTGTTCAGCTGATTGATAAATCTACGGTCTGTGTGGAGTACTGCTGACAGGAGAATAGATAGGGTGAATTTTGGATCTTTGTCACCTAAGGACCTGTCTCCCCAAGAGCTTCTAAGATACCAGGTTTCTTGACGAGAGCAAGGTGACTTTAAGCCATCCAGTGAACCACAAGATCTCTCTGAGAGGCTGGGGAACAGGAGGATGATCAGGTGTCCAGGACACACGAGTCCCTGTGACATGGGACACAAAGACCATGGGGACCTTCCCAAGCTCCCTATGCTGCAGATGCACTGCTCATATGTGTGTGCTGCTCATAGAGACTGAGATCTGCCATGTGGATAGTGTGTGGTGTGCTTAAATGGCATGTAGTATATGTGTGTCTGGTGTACGCAGTGGCTGAGAGGGGTGTGTTACATATCACAGGTGAGTATCATGCATGCTTTGTGGGATATAGCATGTATGCTGCGGAGCGTATGTTGTATGTGGTGGATGATGGTGTGTGACATGTATATGTACTGTACGTTGTACCATATGCTGTGATAGTGTGATGAGTGGCATGAGTGTGTGGGCTAGAGAGAGTACATTCTGTGTGCTGGTCTTTGTGATGTGTTATATGGGGTTGTGGGAAAATGTATTGTGTATTGTTTAGTATGTGTTGTGTGTGCTGTGCTGTAAGGGATGCATGTGATGTGCATTTCATGTTATGGTACTCACACCTCACAACTCACACCAGCACACAACTCACATGTTACATGTAATGTGTAGCAACATGTAGTGTGTGTCTTGGCATGAGAATGTGTAATGATCTGAGAATGTGTACAGATGTGAGGAGTGTGCTGGTGTGAGGTGTGTGAGGTGTATGAGGTGTGAGTTGTGTGCTGTGAGTTGTGTGCTGGTGTGAGTTGTTGTGAGGTGTGAGTAGTATGCTGGTGTGTGTTGTGAGTAGTATGCTGGTGTGTGCTGGTATGAGATGTGTGAGGTGTGAGGAGTGTGAGGTGTGTGCTGGTGTAAGGTGTGCGCTGGTGTGAGTTGTGTGAGGTGTGAGGAGTGTGAGGTGTGTGCTGGTGTGAGTTGTGTACTAGTGTGAGATGTGTGAGGTGTGAGTTGTGTGAGGTGTGTGCTGGTGTGAGTTGTGTGCTAGTGTGAGGTGTGAGTTGTGAGGAGTGTGAGTTGTGAGTTGTGTGAGGTGTGAGGAGTGTGCTGGTGTGAGGAGTGTGCTGGTGTGAAGTATGTGATGTGTGAATTATGTGAGGTGTGAATTGTGTGAGGTGTGAGGAGTATGAGGTGTGAGTTGTGTGAGGTGTGAGGGTTCAGGTGTGCAAGTGTAGCAGTGTGGGGCACAGGCTAAAGGGTGAGATGCTGGGATTACTAGCTCTGTCAGAACTTGGATCCCCATCTGGGATCCTTTGCTCTTGACATCTTTCCGCAGAGGTCTTGTTTGTAAAAGATGGAAACCAGGATTGCTGCTGAGTTTAATGTGGTGGCTCCCATCATTTTACCCACCCCAAGCCGCCACCTCCTTCATGTCTGTCAAGTCCCTTCTTGGTTTACCCACACTGAAGAGGTCTTGGCCAGAGTCTAGCTACAGCATGTGGTCTGACTAGAAGCCGTTGAGTCTCCCTGGAGACTGGATCCTGGCTTTGAAGCCAGGACCTGGTTCAAGGGCATCTCTACCTCCCATGGGCATCTCGCTCTCCCTCCCATGGGCACTTCACTCTTTCTCGGCCTCCCTATCCTCAGCCTGGCCACGTGAGTCACAGTGATACAATCAGTAGATCCTTTCCACCATTTAGTCACATTCTTCAGGCTCCCTGGTCAGGTGGCATGGTAGCCACCTTAGGAATAGGCACTCTGGGACTTCTAACACGGCACAACTGGACTCATCTGTTCAGAAACTCCCTTGTCAGGGCTATGTTCCTTCTGATCTCTGGAGAGATTCATTTTCTTTCCATAGTTCCTCAGACTCTGGGCTCTCACATGGCTTCAGCTCTACACACAGCCATCCTCCGTGTAATATTCAAATGTTAAGTACCAGGCCTGTTGGCTTTGACGAGCCCATGCCTGCTCAGGAGCATCCATTAAGGCAGGAGTGAATGTGAATGGAAGAGCATGTCTTCCTGAGAAGGCAGAGAGCTGTTTGAACCGGAGGAAAGCCATTTCCACTGGAACACTTGGCTGTGTCTCTGAGAAGGGCATGGTGACAAGAAGCCAGAAGTCTTGAGGTTCAAAGAGCTGGAGACTACAAAGGCAAAGCTTGGCCTGTGTGAGAAGAGCTTTTAATAGGATTTCTGAGGCCGGGAAGCAATTTGTGACTCATGCAGTTTTCCTTGGAGGTCAAGTCTAGATCGCAACCATGCATGGTAAGAGTGGAAGGAAAACACCCTTGTTCTCTGAAGGGATGGTGCCTGAACTTGAGGCTGGCCTGTAGGGCCTCTCCCAGCCTCCTTGCCTGCCACCTCAGCCCCCACCCACTCTCTATCTGTGCTTTGGTGGGTTTCCTGAGGATTGGGGATTTCGGGGACTATCAGAGGCTCATTTGTGGCCTAAAACCCTTTAGCTCTGAGCTTCATCTCTCATCCTCATGTTTCCAAAAAGAGTTGAGTATCTCTAGGTTTGCAGGTCTCTGTGGCGCTGTACTTGCCTGGAATGCCACAGGCCCTCTGCCTCTCGGAGGGCTCCTCTGGATTCTCGGGGCCCTCTGCTATGTAGTGGCCGGGAGCCACTGTGAACCTCTGATCCTTGTCTTAAGACCTTCTAGAGTCCTGGGGCTACTGGTGTGAGCATCACCTCTGCTTCGTGCAAAGCTGAGATTGAACTCAGGGCTTCCTGAGTGTGAGGCGAGCTTGTAGAGAGAATTTCTTACCTATGTATGGGAGCATCTCTTGTCAATAAAATGCTGTTGGCCTATTAGCTTATGCAGGGAATAGGGAGGTGGAAATTCCAGTACAGAGAGAGGAATTCTGGGACAGAGTCAGGTGTAGGAGAGATTAGCCCCAAACTCTGAGGAAGATGGTCACATGAACCTGAGGAGAGGTAGCCAACCACGTGGTGAGGATTAGAATAGAATAAATTGGATAATTGAGTTACAAGCTAGTGGGAACAAGCCAAAGCTTTTGGCCTAGGCCATTATTCATACATAATTCACTCTCAGAGTCGTTATTTCAGGAACTGTGTGCGGGTGGAAAAGCCTGTGGTTACAGGAGCACTGTACCAGCCGAGCTGCTCCCCAGGCTCTGCCTGTTGTTTCTGAAGAATAAAAGACACAAGCAAATCAAGAGGTCTCTGGATCACTGAATGCAGCCTTTGGATCCAAAGGCAGGTTTGGAGGTGTTTAGCAGTCGGGAGGCAGCAATTGAGCCCTGGTGTTGCCTTGGTCTGGATTTGGGGTCCATATCTACCTTTTACTGCCATGGAAAAAGGTACGGGGATGTTCCTGAGACTCCTCCCACCTCATTCTTACTCATTTACTGTCCGGTGTAGCCATCTACCTATGCACATCTACCCACCTATGCAGTCTTCCTCACCTCCATCCATCCAGTCACTCACCCATGCTCATCTACCTGTCCATGTTTTACATTCACGATTCCTATCCATCATTCCAAGCATCTACCCACCTACCCATTTCCATATATCCATCCATCCTTATCCAACCATTCATGTACCCACATCTGTCCATCTACCTACCTAACTACCCACATCCATTCCACTACCTATGTACTTAGAATAACCCATATATATTTATCCATTCATGCATTTACCCATCCATGCATTCATCCATCAACGCATCTACCCATCCAATGCTCCATGCATCCATCCATCTCTCTATCCATTCATCCATCTATCCATCCATCCATTCATGCATGCATCCACCCATCCATGCATCCATCCACCCATCTATCTGCATACATATATCCATCTATCCAACAGTGCATCCATCCACCCACCATATCATCCATTCATTCCCCACTCATATTTATGAAGCTACTCAACATCTATCAAAATCCATCTCTCCCTCATCTATCCACCCACAACCTTCCATCCATCCACTTACCTACCATATCATCATTTTCTCCCTCTCCCTCCTTCCCTTTCTCCTTTCCCTCCCGCCCTTCCCACTGACCATACATTTATTTACTTATCTCACCCCCATCTAGCATTTCTCAGAAGTCTATTCCAGTGGACCAGAGGTTACTTAATTGTTGCTCACTGCCACGGAACATTACAGCCTTCAGGAGATACCAAGGACACAGACAGCTTTTCACACAGAGGGTTAAACAGCGTGTAGGAGCCATGCCCTGGCCATAACCCTGTGTCCTGGCCAAGCTTTGGTCCCAGTCCTGGTGGAAAGACACTGTATCTTTCTGCCATGTCCTTTTGCATGTTTCAGTCTGTCCTTCAGTGTGGAGCACCAGCCTTGACTTGCAGGGTAGATGGATGTGTTGGGTAGTTTTAACTTGACATGCACAGTTGTCATTTGAAAAGGGAAACTTTTCATATCTTTGCTTTTGTTTTTAAGAGACAGGGTTTCTCTGTATAGCCCAGGTTGTCCTGTAACTAACTCTGTAGACCAGGTTGGCCTTGAACACAGAGATCCATCTGCTTCTGTCTCCTGAGGGCTGAATTTAAAAGTATGTGCCACCGTAGCTGACTTGGGAAGAGGGGCTTTCAACTGAAAAACATGCCTTTATAAAGTTGGCCTACAGGCATGTATATAGTGCATTTTCTTAATGATTAATGCGGGAAGGGCTAGCCCATGGCAGGTGGAGCCACCCTTGGGCTGGTGGTCCTGGGTACTATAAGAAAGAAAGATGAGCAAGCCACAAGTGAGCAAGCCAGTAAGCAGCACTCTTTCATGGTCTTCGCATTAGTTCCTGCCTTGACTTCCTTCAGTGGTGGACTGTTACCTGGAAGTGTAAGCTGAATAAACCCTTTCCTTCTCAAGTTGCCTCTAGTCAGAGTGTTTTATCACAGCAACAGAAACCAGTGAAGAAAATGAACTTCTATCTCTTCTTGGGGTTCACTTGGACCCAGAAAGGAACTCCCAAGACACCCTTAGCCTGATGTGGATGGAAGGGCCTGGGTATGAAGCTGACCCTCAGTGCAGCATGGGGGGTGGGGTATGTTGGTTGGAGCTTCTAATTTGGGCCTTCTGATGGAGCGGGTATCTTTAGGGCCTGCCTTTGCCTCTAGCTGCCTGTCAGGCTTTGGGAAAGACCCATTTCCTGACCACAGCGACGCCCAGTGTCTGACTGTCTCTCAGCCTCTGCTTTATTACCAATGGAGTGCTCTCAGCAAGGCGATTCACTGATGAATCAAGACAGCAGTTCTACCACACACCGACCACATGCCAGGCCGTGAGCTGGCACCTGCCACTCACAAACTTGCTGCCTACCACAATCCAATCTTTTCTCCTGAGAAACTGAAGAATAGAGAAGTTGAGTAACGTTTTCATTCACACAGTTAGGGAGGGGTTTGAAGCCAGGGAGGCATTGGCATCTTAACCACTACCCACTTACACCATTAGCTCCTATGTTAATGGGTGTTAGCTCATGGCAAAGAGAACTGTATGGACAGAGAGCATTGCTTTACAGGTACAGGGGTCCTTGGGTGACAAGCTTTGTGACATAGCAAGGGACCCTGTTGCCCAAGAGGTAAGTGACCTCTTGACTTGTGTTACAAATCTAGTACCCCAGGTCCTGGGGCCTGGCAAGAATGAAGATATTCTAAAGTAGTGGTCCTTTAATTCAGTTCCTCCTGTTGTGGTGACCCCCAACCATCAAATTACTTTCATTATTACTTCATAATTGCAATTTTGCCACTGTTATGAATTATAAATATCGGATATGCAGGATATCTGATATATGACCCTTGTCCTTCAGTCCCCAAAGGCATCATGACCTGCAGGCTGAGAACCATTGTTCTAAAGGGACATATGTGTCCCTCCCTTTGGCTTATCAGACCACTGAAACAACAGATGTGAAGAAACTGGACATTTCACCAGGATTCTTAGACTGTGAGAAGAAAAAGGCAGAAGCCTGGTGCTAGCCAGAGGGGGGGCTGGGCTTGTTTTCTGAAAAAGATCTCACACAGTTCTGACTTGTCAGACCCTTGCATGAGAGATTGGAGTCCAGACAATGATGGATTGGCGATGGTGGTGGTCGTAGGGGCTGACCCTGGCCAGAGCTGTCTAACTATGGATACCCTTTGCCTTCTATTGAAACAAACCTCATGTCAGGGTTTGGGGTAGGAGTGGGTCTCCTTTTGTTCCTCCCCCAAATATGACCACATTGAACAAACCTTCCCGCCCTTCTTGCTTTCCCCTGTTAAGTTTGCCTTTGATTGATCTACTGAGGTTGAGTGGGCAAGCCTGGCTTGTAAGGATGCTGTCAGTATGTAGACCCTGCTCTAACACCAGTTAACATGGCTTCTTCCCCAGCCTCGGCAGCCACACCTTCACCCTGGGTAGACTGCTGACCTCTGACTTTGATGCCACCTACACACAGCTTCAGCCCATCTTATTGAAGACACCACCAGAGTCCCCTACCAGCCTCTGAATCACTGCTCCATAGAGATTCACCACTGTAGCCAGAGGCTTCTCTGCAGTCCTCTAGACAGATCCCCCAAACTCCTTCTGACAATAAAGGACACACAGGCATCTCACTCCAAACTCACTCCATGGCCACCCTCTGCCACCCTGCTCTGGGGCTGCCTGCTCCTCTCTGCCAAATCTGCAGGACAAAATCATTAGCTGTGTCTCGTTATTTTTGGAAAGCTCTGGGGACTGAATAAAGCATCTCTGATCTCCCGAACTGGGGGAAAGGAGATATGGATGTTGTCATTTTTTGGTATCCTTCCCGCCTTGGTGTTCCATTACGCGTGTTCTCTAACCTGCTCCCCCTGCCCTGCCCATTGTCACTTTGCTTTCCTTAGCCCGCAGCCCCAGTGTGGCAGGGATGGCTGCTCAGCTGGTGGGGGTGCAGGCCCTGCTTCCTCCTTCCTTTACCAGCCCTGCCCCACTGCTGCTGACTCTCATGCTCTTCAATAACACACAGCTCCTACAGACCCACGCTGACGTGTCCCTCAGGGACAGGAGGCGAGGTCAGGTTTCCATCGCATTTGAAGGGTTTCTCAGCTGCACAGAAACACCCTACAGCTGGGCAGCAGAGAACATCTGGATGAGAGACATCTGCTTAATTGCCAAGAGGCCACCAGTGCTTCTCAACCAATCAGGGATCGCAGCACAGGCTAGTTAGCTGAGACAGGGAGTTACATCTCATTCAGGGGGATGTTTCTGTAACCAGGAGTGACAGCATCCTCTGAGAGACATGCCTTGTGAAAATGGTCACCCTGATTTCTCTCTCTGAACCCTGGCTTTTCCTATCACCTCCCTATGAGAGCAGCATTGGCCACCCCACTCAGACTAATCTCCTGAGGCACACACACACTGACCAATATACCATAGTGGCAGATCTTAGCCCAGCTCTCCAGAGTCCCTCACAGGCCCTGTTCTCTCAGAACCCTGCTGCCTTTCTGACTAGCCCTGACTAGCCCGGCAGAGGAGGCGACAGCATGCAGAGCACAGCTGAACTAGCCTGGACGGAGTGCTACCCCTCCGTATCAGCACAGCTGTCTACCTGAACTGAGAGGCACGAGGAAGGCTGGAGATGTGGGAGGAGGCTCAGTTTGAATGCTCAGCGTCTACCCACAGGGTCGAGGTCTCCCACTAAAGCCCACAGATGCTCACATTCCTCAGATAAAATGTCATCATAGTAGAGCCCATGCAGAACCCATTTCAATCCTTCTCTTGATGGCTGAGACCCAGTGCAGGGTAAACACTAAGAAATGGCTGTTTATCTGCAGTTAGGGTGTAAGCACAAGAAATAAAGCCTGCAGATATTCAATGTCCATGCAACTCAGCACAGGGCTGTTTAGTTATACACACCCTCTGTCCTTTAAATATGAAGCTCATGTCAGGACCATGAAGGTGAGCATGGGGTGAAGTTTCTAGACCTCTTTGTTCTTCCTAATGTAGCTACACTAATACTTTGTTCTTCCCACTCTCTGCTCTTCTCTTTTAGTTGGTCTTGGGCTGGACAACTGAGGACAAGTGGCCAAATGTGGCTTGAAATGAAGCTGTCAGGGCCCTGGCTCTGATGCATGTATTTTTGTGTTTGTGCATTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGATGGCATGTGTGCAGGCAGATGTGTGATATGCACGCATATGTTGACAGCCAAATGACACGGGGTGTTTTCCTCAGGAGTCCTCCAGCGTGCTTTTTGAGATGGTATCTTTCCCTCACTGTAACTTGACAAGCAGGCTAGGCTGCTGGCCACTGAGCCCCAGGGACCCACCAGTGCTGGGATTATAGGTGTGCTTGCCCACACCCAGCCCATGTATGCCATGGCCCACATGTGGAGGTCAGGGGACAACGTTTGGAGTCTGTTTCTCTCTTGTTACTGTAAGAGTACTGGAGAGTGAACACGCTAGCTGCACTTAGCAGCAAGATCCTTTACCAGTGAGCCACCTCACTGGCCCATATACCTGGGTGCTGGAGCACTGAATTCAGGTCCTTGTGTTTACCAGATAATTGCTGTACCTGCACCATCTTCCCAGTCCTCTTCCTACTTCTGAGCCAGCATTGATTGAATCAATGGGAGCCTGGAGTCTGGTCACACAGAATGCAGACTCTATACAGCTGTTTGGAGCTCTGACTTGAAGTAGTTAGATTACGTGGCAAAAGCTAACCAATTATGGCTGGATGTGGTGGTGCAAGCCCTTAATCCTAGCACTCAGGAGGCAGAGGCAGGCGGATTTCTGAGTCCTGTGCTAGGGTTACAGACTGAAACCATGTCCCAAAAAAGGTGTCTACTTATTTATTTTTATTTTTTAAGTGTGTGTGTGTGTGTGTGTGTTGAGGGATCAATGGCAGGATTTAAAAATAGATATCTAAAACAGCAATCACCAAGAAACAACAACAACAACAACAACAACAACAACAACAAGTTTCTTTTGGTTTCACAAACACTGCAGCCACGTTAGTAGAAGGCTAAGAACGGAGCCACCTATTTTGTGAGCCACCCAGAATCAGCAGCCGAGCCAGGTGCTCTGTTATGGAGGACACTGCCCCGTGTCTACTTACAAGGATCTTGGTGGTGTAGGCGCTGTTGATGGCAATGGCGTTGACCAGCAGCTCCAGCGTCTTGGCATTGATGGAGCTGGGATCGGGGATCTCCTTGTAGTGGACGTCACCGACATAGGCCTGGACCACGGTCATGCGGTTGGTGGTCAGCGTTCCTGTCTTGTCTGAGCAGATGGCTGTGGCATTGCCCATGGTCTCACAGGCATCCAGGTGGCGTACCAGGTTGTTGTCCTTCATCATTTTCTAGAGAGTGGATAAGTGAGAGGGAAACCAAGAGGAAGAGAGGGAGGGAGTGAGGGCATTTCTGGGCCTCGGCCTCCTCTTAACCTTCTGCCTGGGTAAATGACTTCTGTCCATATTTAGCTTCATATGTGGGTCTCCTTTTTACTAGTCACTTGGACCATTTAAGTTTTGATAGATGAATTTATTAATTTGCATCCCACAAGTGACTCATTTTCTATTTTGGGAACAGGATCTCATGTAGCACAGGTTGACCTGGAACTAGCAACCAAGCCAAGGAAGACCTTGAACTCTTGATCCTCCTGTCTCTATCTCCTCAGTGCTGAGATTACAGATGTGCTCAACCACATCTGGATTCATCCGCATTCATAAAAGCAAAACTGTTTTAAAGAGATTTATTTAGTTTATGTATATGAATGTTTTACCTGCACCATGTATGTGCCTGATAGCCATAGAGGTCAGAAGAGGGCATCAGAACCTTGGGACTGGAGTTACAGATAATTGTCAACTACCATGTGGGGGCTGAAGCTGAACGTTCTGGGAGAGTGACAAGTGCTCCTAACCTCTGAGCCATCTCTCCAGCCTCCTCATCTACATTCATGTTGGAAAAAATAGTAGAAAATATTTAAAAGAATAAGGCTGGGCTATCCCTAATTACTCCTAGCTTACTGTAAGTAGTAGCCTCTTCTCATTTGGGTCTGAATTTCTCAAATGCTTTCCTGCTGCCTGCTGAGAAATCTACCCATTTCACCCTAAGTAGATACATTTAGGATACATACATTACAGATACAGGAAAATATCTGTAAAATAGGAAAATAATAATGTCATTGTCACTAGGTGATAGAGGAGAATCTCACTGACCTTGTCAAGTCTATAAATCAACTTTTCTGTAATGAACACTTACTATAGTGTGCATAATAAAACCATGTTTGTACGTGTTTGAGTGTGTGAGTGTGTGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTACATACGCGCATGGTGTCACTGGCATATGTGTGAGATGGTAACAGGAGCCTCCTCTGTGGCTCTCTGCTTTAACCACTTGAGACAGAGTCTCTCCCTGAACCTGGAGTTCTCTGCTTCTTTGGCTAGACTGACTGGCCAATAAGCTTCAGTCACCTTCCAGTCTGCCTGCTCTAAACGTGGGGGCAGGGTGGGGTACAGGCGCACGCAGCACACACAGCTGTTTACATGGGCACTGAGAGTCTGACCTCCGGTCGTCAGGCTTACACAGCAAACACTCTTACCCACAGAGCCATTGCTGCAACCCCAAGTACACAGGTGCACTGCCTAACCTACCAGCTCTGCCTAAACTTCCAGCTCGGCCTAACCTGGCAGTCTGGCTCATTCTACCCACCGGTTCACCAACCTGCCTTACTGAAGGAGCGTTGGCCCTTTAAGAATCTCTTTTTGGAAGCTAGGTGTGATGACACGTTCCAGTACTTGGGAGATAGAGGCAGAAGGATTGTTGTTAAGTTCAAGGCCAACCTGGGCTGCACAGGGAACCTCTATGACAAACAAAACAAAATCCCAAACAAAATGCCCAAAAATTCCTCACCAGGACTGTCCAGCATGGTAACTGCAGGGGGATGAGAGGCACTTAAAGTGTGGTCCAAAGTGTGTGAGATGATAGATTTTAAAGACATTATCAAACAGATAACCAAAGGTAACATTTCTCGTGTTGTTTATGCGTTGGATTGTTGATACCTTGGATGCAAGTTTGAGTAAATTTGTATGATTAAGATTAATGTTACCTGTTTTTGAGACAAGGGCTCATGTACTTCATGATCTTGAACTTGCTGTGTTCAAGATGACCTTGAACTTCTAATTTTCCTGCCCCATTTCACAAGTATTGGGATCACAGTTTGTGTATCTCCACGCCTGGTTTCTGTGGTGCTGGGGATCAAATCCAGAGCCACTTGCATACTAGGGTAGGCTCCTACCCCTGAGCTAGAATATCCCTAGTCACACTTTATATCCCACTCGTATATATGGATATACCTGCCCATATAGGTACAAGTGGAGGTTAGATATTGACATCAGGTGTCCTTTCTGCCTTGTTTTTCGAGACAGGGTCTCTCACTGAACCTAGAGCTTGCTGTGTCAGCTAGATCGGCTGGCCAGCTTTCAGGATCTGCCTGTGTGGGTCCCTCCTGAACTGGGGTCATAACACTGAGGTGACAGGTGTATGTGTCCAGGCCTGTCCTTCATGTGGGTGCTGGGATCTGAACTTGGGTTTTTACACTTACACAGCAAGCACTTTACACACAGAGACAGTTCTCCGGCCCTATTTTTAAAAAGTTAGTTACTGGGGCATTTAAAACGACATGTCTGGATTGGGTGACATCCCTGCTGAGAGGAACTGATGTACTTACACGCTCGCTACTCTGCTCATGTGGCTTTGCCAGTCACATGACCCCAGATGACCAAACCAGAGATAAAAACCTCAGAAGATTCTCAGGCTTAAAACTGGCCATTCATGATGTCCCCAAACCAGCTTTGCCAAACTTCACTGAGACCCTGAGTTCAGTGGTGTGGCAATGGGGATCGACTCATGGTTGTGTAAGAGTTATCCTGGCCGTGCAGGCCTGGAGAAACACTGTGTTATGACTTGTTCAGGCTTTGGTTTGTGGGAGGGGGGTTTCTTTGAGAACTGTTAAGAGAAGGAGCTGATCAGCACTGTATCCTTTACACGGCACTGCCCCTTGCTGGCCCCTGTGGGAGATCACTCTCTATATGAAATCACACCATAGGGTGCAGCCTAAATTCTGGCTGGGAAACTGACACTCGCCTTGCCCTCTGGCTGAGTTGCTGATGTCTCAGAGTGAAAGTCTTTTCAGTGATATCTTGGTGTTGAGACCATGAGAAAGAGGTGTCCTCATCCCTAGCCAAAAGGATGGGGACGCTCAAGGGGAGAGGAAGAACATGGACAGGTAGGACAATCTGGTGGTGGTACCCTGGGGGCAGATTTGTAGCACTTGGACTCTCTGATGGCCAGGGCTTCAGCAGGTACGTGGGTGCTTAGGGAAGGTCCTGGAGTCCCGCTTGACCAGCGAACATCCAGGTGGTATGCCATTTAAATCCTCCCGTGTTTTCAGCAAACCCTGCCTGACGGGTAGTGGCTTCCTGGGTGGTTTTGCTCCCGCATCCCACACGTTAGGGCATGACCTGTTGGGGTTTGGTGGGATGGAGTTGTGTTCTGCCTTGCACAGTGATGTCTACATTCGACAGTTTGCAGTACTGTGGCCTCGGGCTTCACTGAGCCGTACTCCGGTTCCATTTTATTTTGTAAGTCTGAGTTGTCAATACAGATCTAGAGAGTTTATATTTTGTATAGATTTTTGGGCTTCTTTTTGAGAATAAGTCTGAAGGTCTGGCCA
GF3 -1 -1 1 GACCTCCGCAGGCACCAGGCACACTTG
GF4 -1 -1 1 CTCCGCAGGCACCAGGCACACTTG
EX52 -1 -1 1 CGTGGACACCTTCGTGGTCAACAAGAAGC
EX32 -1 -1 1 GTCAGCCATGGCTTCTTGTTGACCACGAAG
GR3 -1 -1 1 GTGGACGTCACCGACATAGGCCTG
GR4 -1 -1 1 GCACAGCTGAACTAGCCTGGACGGAGTG
Comments:
Created
Target: 2803Ensembl ENSMUSG00000030302 -- 113743660 -- 113743874 ENSMUSE00000330089 -- ENSMUSE00000330089
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:6:1:149517037:1, start 113743659: end 113743874
Found 10 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
U51:6:113744294:113744413_28 was rejected with 3 alignments
U32:6:113744234:113744353_70 was rejected with 3 alignments
Entered all non-repetitive oligos
Sequence count reduced by 2 by bac-alignments
finished aligning oligos to bac
ACCEPT (0) gap pair G5:6:113749374:113750373_665 G3:6:113739160:113740159_937
REJECT (1) gap pair G5:6:113749374:113750373_665 G3:6:113739160:113740159_929
ACCEPT (0) gap pair G5:6:113749374:113750373_665 G3:6:113739160:113740159_942
ACCEPT (0) gap pair G5:6:113749374:113750373_769 G3:6:113739160:113740159_937
REJECT (1) gap pair G5:6:113749374:113750373_769 G3:6:113739160:113740159_929
ACCEPT (0) gap pair G5:6:113749374:113750373_769 G3:6:113739160:113740159_942
ACCEPT (0) gap pair G5:6:113749374:113750373_700 G3:6:113739160:113740159_937
ACCEPT (0) gap pair G5:6:113749374:113750373_700 G3:6:113739160:113740159_929
ACCEPT (0) gap pair G5:6:113749374:113750373_700 G3:6:113739160:113740159_942
DESIGN COMPLETE
U-type: U12
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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