High Throughput Gene Targeting: Create Design

This custom vector design tool is provided by the EUCOMM consortium as a service to the research community. When using this tool, the user agrees not to submit more than five designs per day for creation without prior discussion with the High Throughput Gene Targeting Group at the Sanger Institute.

Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by dk3
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 183424
Chr: 17
Strand: -1
Status: Ordered
BACs: RP24-322E3 : RP24-545D16 : RP24-540O5 : RP24-459G19 : RP24-265I23 : RP24-226P12 : RP24-362I22 :
Instance: Plate 154: Well D07: BACs RP24-322E3 RP24-545D16 RP24-459G19 RP24-540O5
Oligos on GRCm38:
Type Start End Length Seq
G5 24164358 24164407 50 AGGGGTGATCTTTACATCCTATCCTGTGACTGATGGTGGAAAAGGACTAC
U5 24159167 24159216 50 ATAGGGCTTAGCACCGCAGCCCAGCCCACTCTGATCAGGATGGCCACAGC
U3 24159010 24159059 50 AAAAAATTACTACAGCCTCCCTAAGCTGCAGAATAAGGAAGCCAGGATCG
D5 24157342 24157391 50 GTAGCCTGAGCTCTACTTGGGAATGAGCACGGTCATTTCAATCTTCGAGA
D3 24157206 24157255 50 CCCTGCAGATTGCCATTATCCCACAGGTCTAGTCCTTGGCCTTTCCATGA
G3 24153819 24153868 50 TTGTTCTTATTATTTCTAATTATACGTATGTGAGCATGTCTGTGTGTAGG
Oligos:
Type Start End Length Seq
D5_D3 24294223 24294308 86 GCAATGTGATCCTGCTGCTGCACGAGGGCACAGCTTCACGCTGTTCACCACCCTGGCTCCAATGACTGGCTGCTCGCCATTCCTCC
G5 24301325 24301374 50 AGGGGTGATCTTTACATCCTATCCTGTGACTGATGGTGGAAAAGGACTAC
U5 24296134 24296183 50 ATAGGGCTTAGCACCGCAGCCCAGCCCACTCTGATCAGGATGGCCACAGC
U3 24295977 24296026 50 AAAAAATTACTACAGCCTCCCTAAGCTGCAGAATAAGGAAGCCAGGATCG
D5 24294309 24294358 50 GTAGCCTGAGCTCTACTTGGGAATGAGCACGGTCATTTCAATCTTCGAGA
D3 24294173 24294222 50 CCCTGCAGATTGCCATTATCCCACAGGTCTAGTCCTTGGCCTTTCCATGA
G3 24290786 24290835 50 TTGTTCTTATTATTTCTAATTATACGTATGTGAGCATGTCTGTGTGTAGG
G5_U5 24296134 24301374 5241 ATAGGGCTTAGCACCGCAGCCCAGCCCACTCTGATCAGGATGGCCACAGCCTTAGGAAAGAGTTAGCAGCACCTACTTGGCCCTTGGAGATGGCCCAGTATAATCATAACCCTCCTTTCAGACCCTCAGGCGACACCCTCTGTACTGGTTGGTTTTGTGTGTCAACTTGATACAAGCTGGATTTATCACAAGAAAAGGAGCTTCAGTTGAGGAAATGCCTCCTGAGATCCAGCTGTAAGGCATTTTTCTCAATTAATGATCAAGGGGGAAGGGCCCCTTGTGTGTGGGACCATCTCTGGGCTGCTTGTCTTGGGTTCTATAAGAAAGCAAGCTGAGCCGGGCGTGGTGGTGCACTCCTTTAATCCCAGCAGAGGCAGGCGGATTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAGATAGGAGGCACAGCACTGCTTTTAACTCTAGAGAAGCTTTGTGTAGTGGAGTGCATCTGTTGTCCCCATACAGGAGATCGTCTGAACCCAGGAGTTAGAGATCAGTCTACAGTAAAGCAAGACGCTCTCCCAAAATAAAAAGGGTGCAGGTCACTGGATCCTAGTAGTTTGGGGCTTGGAGACTTACTGCACTGGTAGAGCACCTATTTAGCATGTACAAGGCCCTGAGTGCAATCCCTCAGCACCATAGACGAAAGTTTAGGGTGTTTTTTTTTTGTTGTTTTGTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGAAACTCACTCTGTAGACCAGGCTGGACTTGAACTCAGAAATCCACCTGCCTCTGCCTCCCAAGTGCTGGATTAAAGGTGTGCACCACCACGCCCGGCGAAAGTTTAGTTTACGAGTGCTTGCATATGTTTGTAAAGCCACGTGCTTGCCTGGTTCCTGTAGAACCAGAGGGAGTGGCAAATTCCCTGAAACTGGAGTTACAGGCAGTTATGAGCCACATGATATGGGTGTTGGGAACCAAAGCTGAGTCCTTTGCAAGAGCAAGTGTTCTTAGCTGTTTGCCAGCCCCAAACACACATTTTCTTACCTTCACCTCTAATCCAAACCGTGGATCAGGAAAACAAAGGTATAGCTAGGAGTGGTGGCACAGGCCCTTAATCTCAGCACTCAGAAGGTAGAGACAAGCTGGCCTCTGTGGTTCTAGGGCAGCCTGGTCTGTACAGTGAGTTCCAGGATAGCCAGGGCTACCCAAAGAGACCCTATCTCAAAAACAAACAAACAGACAGACAGACAGACAGACAGAGAATAAAGACAAGGCTAAAAGGATGGCTGAGTGCTTAAGAGCATGTACTACTCTGACGCAGAGGACTGGATTCAGTTCTCAGTACTCACATATTCACATGCAGACATACAAACCTACATAGAATTAAAAATGATTAATCTTCAAAGAAGCAGCAGCAGCCGCCACCACCAGATGACTTAGTATCTTAAAAACCAAGCAAAGAGCCAGGCATGATATTATGTGAGAGGCTGAGACAAAGGGCCAGCCTCAATTACAAAAACAATCTGGACTCTTTGAGACCTTGACTCAAAGCAAATTAAAAAACAAACAAACAAACAAACAAACACCAGGCAAAATGGCTGAGTGGGTAAAACGCACTTGCCAACAAGCTATCTCATGACCTGAGTTTAATTCTCAGGATCCAAAGGATGGAAGGAGAGCTATGATGCCCACAAGTTGTCTTGACATCTGCTTGCATACCATGCACTGCATGCATGCAGGCCCACATAGGTTTTCGTGCTCACACACACACTCACTCACACACTCACATACACACAATTTTCTAGAAGAGCACTTTGAAAGGCAAAGTGAGAGCATGCAGCAGAAACGAGATAAATGAGGCTGGAGAGGAAGGTAATGATGAATCAGGGGTGGGCAGGGTGGCAGACATCCTGTCTGAGGTGCCTGACCTTCATCAGCTAACAGATCTGAAGCAGAAAATCATCTGAAGCCATCAGATCTGTCTATGTCTGAAGCATAGGCTAGAAGAGTAAATCTTAGAGAAAGAGCAGGTGGTTTCAATGAACCGGATCTTGATGAAGATACCCAAGTAGTAGCTGGGCAAGTGAGCAAGCCCCTCAGTGAGAAGGGCCCTGGAGGAATTAGGAGACATAGTAGGGAGGGTGTTTGAGGGTAGGTCTCCTGATGTCTAATGCAACGGGGTATTTCGTTTTTAAACTCAAGGCTGCCCAGTCTCAGCCATCACCTCCTACCTGCTTCCCAAGGCCTTTAGTCTAAAAGTGTTTTCTGAGAGTCTGTGAGGTAGCTGGACTACAGTGGGGCACCAGGGTGAAAAACCTTAGGACTATTCCTACTAAGGGAGAACCCCAGCCTTGCACCTTGCCAGTTGAGCCTGCTGCATGAGGAAAACACAGCGGTGGAGAAGGGGCGTGGGGGCAGGGCTCACAGGAGCAAGGCCTTCCGGGAAGCGCCCAGGAGGACCTGGCACACTTATAGCTGCTGGAATGTGCCTGAGGGGAAAGAGAAGCAGCTTAGCTTAGCAAGGGAATGGTAGTAGGGGGCACATCCTGACACCTGTCACCTTGATAGACGACTCTGGGTTTTGCCAGAGATCAGCTGGGAGAACCAAGTACCTTTCTGCAGTAGGACCATAGGTTGCCCTTGTAACCGTGGAGCACTGTGGGAGAGTTCCAGAAAGACATCCCTTGTTGACATTCAAGGAATGCTTGACATTGTCAACCACCCACTGACTGTTCTCACTCTCCATTTAATGAAACTGAGACTCAGAAGTAAGGTTGTCTTGAGTCTTCCAAGGGTGGCAAGTCAAGGGGCCAGAGTGACGCCTATGCAGCCCAGGGCACAGCCCACACTCAATACCCACAGTTCCCTCCTCTGCTTCTGTGTCTACACAACCAGAAAGGAAGCCACGGGCAACCACACACTCCCTGATCCTGTCCTGGAAGAAAAACTGGCTGCGGCGATTGACAACAGGAACAGAGAAAATCCATTCGAGTATGGATAACAGCATTCATTTACTAAATTCGTCAACTGTGTTGCATAAGAGAATACCACGTTTTTAAAGCAAGAGTTCAGGTCTAAGGGATAAACCCGCAGAGAACACATGAAGACAGCAACCTAAACAAGCTTCCTTAAAACTGGGAAAACTAGCCTGGCGTGGTGGCACACGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCAGATTTCTGAGTTTGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCAAGGTCTATACAGAGAAACCCTGTCTCGAAAAAACAAACAAACAAACAAAAACCGGGAAAACTGTGGGAGAACCTTACATGAAATTTGGAACAAGACAACAGACCTCCTGCTAAGATAGAAGGTGGAAGGGTGAGGACAGAAGCCAGTCCACAGCAACAGCAGCTCTTTCTGAAAGGCTTTTTCATGTGAGTCACACAGCATAAGCAGGTTAGGGAGGAAGAGCTAGACAAGAGACAATGGCTGTGGCAGAATTCCAGGCGACCTTAGTTCAGGAGACCCAGAGGCTGCCTACAGGGCGTTGGGAGGGCGTGGCAGATGAGAACCGGGGCAGGAAACGTGAGGACAACCGCACTGGCAGTTGCAGAGACCAGGCAGAACCCAACTGAAAGTGAGGCGGATCCCCCTCGGACCCAAGTCCAGGAAGCCCAGATTCGGGTGAAGGAAGTGGTCCTTTCCTGAGGGCCCCTTTGCTCAGGGGTAGAACGGCCTCATCTCACCTTGTGATAAACCTCTGGTGGGGAGGTGACCAGGGTGGGACAGAAGGAGGTGACACCGTGGGACAGGAGCCTCCGGGCCACTAGGGCGACCCCCGAACCCACGTCCTCCGTAGCCTTGGAGAAGTCAACACCAAATCCACCTGTGGCCACAGAAAACAAGGGGGGTAGCACAGTGCTCGGAGCTCCTGCCCTGGGTTCCCTGGCCCGAGCCAGGCCGCACCGTTGATCTGCACATCGATAAAGCCAGGCGCCAGGATGCGGCCTCCGCAGTCCCGCTGCTCATCAGCCACACGCCTCTCCTCAAAAAACAGCTTTTCTGGGTCCAGGATTCGGCCCCCGCGCACCCACAGATCCTCCCTGAGGGCAGAGTAGTAGGCACTTGGATCACCACCAGGCCCTGGAACCAAACCCTAGTTCTATCTCGCACCCAGCTTGTCCCTGCACCCACTATTCTTCTTGGTCCCGGCCACACACCCACGATAGTCCCGCCCTCCACATTCCTGCCCCTTTCAGGGCTTATGCCCTACACTATCCCCATCTCTGCACCCCGCACTGGCGTATCATCAGCTCTATACCCAGCCTAAGCCTGCACCATGCAGCCCAGACCTGAGGAGCGTGCCGCCACGCAGGATGCGGCAGTTGGTGAACTGGAGCACCGGGGCCCCCGCTGCGCACTGCCCGCTACGCATCGTGAGTCGCGCCGAACCCAGCAAGCTTTGGGCGCAAGCCGGCAGAGCCCGGAGCAGCCGTAATCGGAAGCGGGGCCAGGTCGCATCACGTGACTCTCAGCTGAAGGGGCTGTCACGTGGCCGCGGTGCTCACGTGGTGGCGACCACATGATGACAAACCCTGCCAGGCCCGCCCACAGAAGCGCCAGAACACAAGCCAGTCACATCCGAGAACTTTTATTGGACACAGCTGGGAGCCAGCTCTGAGGAGCAGTAGCACTCTATCCTCCACCCTGGCTGTTCAGGGCAGGGGGAAGGGTGGCTCCATCCAAGGAGTCTCAGCAACAGGAACCCCACAAGGCAACAGTCTACTAATGATTACTATATAGTTGGGAGACAGGAGCTGCTCCACAGACGCATGAATAGGTCAAATCTTTTATAAATAAACATCCAGTGAAGAGAAAAATGACAACTCTAGTTCATTCTTATACACAGGAGCACTCTAGGGCGATGGGTGGGGTAACCAGAGGCTGACTTCGTCACTGGTCAGGGGCCTGGGTGGGAGATGAGTGGGCCCGTATGTCCACAGCACGGTGCAGTAGGGGCAAGGCGGGGGCGACAGACAATGGGCACTAGGACACTGCACATTTACAAGACCAACTACTGAGGGGCAGCTGCCCGTGCATGTGTCAGGCTGTTCGTTCTGGAATGAGGAGGGGTGATCTTTACATCCTATCCTGTGACTGATGGTGGAAAAGGACTAC
U3_D5 24294309 24296026 1718 GTAGCCTGAGCTCTACTTGGGAATGAGCACGGTCATTTCAATCTTCGAGAAGTCACACCTCCCCATCTCCCAGGTTCCAGGTAGTTAGAAGATGTCAGCACAGGGCTCCAATTTCCCTGCCTCCCTTCTAGCTTCCTCCAGATACAGCCTCTACCCTCAGACACCCAGGAACACCAGCCAACTGACCTGTAGCCTGCAGGAAATGCCGGACGCACACATCCATGGGGGCTATGCTGCCACCCAGTGTCTTGGTGCCTTGGAAGCAGAGAGCCAGAATTGGAAGAGAGAGTTGCTGTGGGACTGGCACCCCAGTGGACAAACAGGGCATACCCAAGTTCTGCTCCCACTCCAGGGAGGAGCCAAGTGACTATACATATATGGATACAGGAGTCACTCACCTGCAATGTACGCTATTAGCCCATCTACCTCCACCTCCTGCTGTCCCAGTGTATGGCGGCCATTGCCCAAGCCCAGGGCAGGGACAGCATCAGTGACCAGCACCAGCCCTGGGGGAGAAGGTGGCTCAGGACCTGGCCAGCTGTTATCCTACCTGCCCTACCCTGCCCAGCTACTCACCCTGGGGATGGGCCCGGTGAGCAATGCGCAGAGCTGCAGGGTTGGTGTGTATGCCATCAGCAATCATCCCATAGAAGATGCAGTGGCCTGGGGGCAGCTGGTCACTGGTCAGAAGCCCTACTATGCCTGGGTCACGGTGGTGGAACTGAGCAGGGCAGAGGGAATAACTGAGTGACAACAGTGGCCACTGAGTCCCCTTCCTCCCAGGAGTAGGTGTGACACTCACAGGCAACATGGCATTGAAGAGGTGAGTGATGAAGGTGGCCCCACTCTGCACTGCCACTTCTGCAGCCCGCAGGTCGGCCACTGAGTGCCCTGCGTGGGGACCTGAGATCAGTTTCTGCAGCAGCAGGAACACCGTGAGCAGACAACCCCAAAGAGAGGCCTCTTACCTAGAGACACACGGATGCCTTGGGCTGTCAGTGCCTGGATCACTTCATGGCTCCGGTCTAGTTCAGGGGCAAGTGTCACAATGCAGACGTTGTCCAGGGGCCCATATGTGGCCAACACATCATGAAAAGCATTGGCTTCAAAGGAGCGGAGGTAGGCCTCGGGGTGTGCACCCCGCTTTTCCCGGCTGATGAATGGGCCCTCCAAGTGCACCCCTGAGGGAAGGGACAGAGGTAGTTCTAGCAGGCACTCCCACCTAGCAGCCCCACCCCAGTCCCTCAAACCTGCCCTCCCTTCCCAACTGGGCATTTTGAAAGGAGTTAACCACTCACCGAGGACCCCTGCCCCATGGGGACCTCCACTCTTCACAGGGATCTGAGGAAGGACCTGGGGGGAACCAGCTGTTAAAGCCCTGTCCCCACAGCCCAGCACCCAGAAGCCACAGCCCAACAAGGTCAGATCCTCATCTGACCCAGCCCAGCCCGACTGGCCTTCCCCACAGGAGCTGAAGATGAAGGCCCTCCTCCCCCAGTGCTCAGGTGCAGGAAGACCTGACAACCAGGACTACTTGATGACCTACCACAAGGGCCGGAAGGTAAAAGCATTCCCAGGCCACAGAAGCAGAAGGACTGCTCTAAACTGTTCTAGCCTTCAAAGTGGCTTTGGCCAAACAGCTAGAATATAGATGCCAAACTCTTGAAAAAAAAATTACTACAGCCTCCCTAAGCTGCAGAATAAGGAAGCCAGGATCG
D3_G3 24290786 24294222 3437 TTGTTCTTATTATTTCTAATTATACGTATGTGAGCATGTCTGTGTGTAGGGATGTACAGGTGCCCTCACGGGCCAGAAAAGTCAGAGCACCCAAACCCCGAGTCCTAAGCAAGCTACTGACATAGGTGCAGGGGCTTGAACACAGGTTCTCTGGACAAACAATAAGTGCTCTTAAACACTGAGCCATCTCTCCAGCCCCATCTTTTTGTTTGTTTGTTGTTGTTTGTCTTATTTTGAAACAAGGTTTCTCTGTGTAGCCCTAGATGTCCTGGAATTCACTCTGTAGACCAGGCTGGCCTCAAATTCAGAAATCTGCCTGCCTCTGCCTCTCAAGTGTTGGGTTTAAATATATGTACCACCACTGCCCACTTTAAAAAAAATAATAATCCAAATTCCTAATTGCAATTCCCGAACCCCTGAGCCTTCTCCCAGCTGTTCTGCCAACAGGCAGACCTGCTTGACTCTGTCCTCTGCTGAGGCCGTCTCTCCAGCCTCACAACCTTCCCAGTGCTAGCTCCATCATTTCTGGGCAACCCCACAACACATAGCTCACTCATCTGAGCCCCTCCTGACTGCCTATCCAGTGCAGCTGCATGAATCCACTGAAAGTGGACCTGGGCAGAGCAGGGCCAGCTTTGTACTAGAAATAGAACTCAGGACTACGTGCATGTCAGACAAGCTCTCCACTACAGAACTGCACCTTGACCCTCCTGGTCAACATTCATAAAGAGTTTGAGAGTGAGACCTTCCACCAGTGGGAGGTTTCTACAGGCTGCTGAGTCCTGCTAGACTGGCCTCACGTGACACATGCTCCCCCTGTCTTTTGTGCTTGTCACACTCCAAGAGTTGGTCAGTCATGGAGGGGTGTGGCTATGGCACAGACCCAGGTTCTCTTACTTACCTCTCTCCTCATCGAGGTTGCCAGCATGCTGGCTTTCTTGGGTTGAGAGCATACCTAGTGACTAGTGGGTGGTGGCTGGTCCTCATGTGCCCCTGCCTACCAAGTTACTCTCCCCAGGCTGTTGCTCTCAGACAGTGGGAGGCCCAAGAGCATCTTATTTTGTCTGCATATTTTCATTTTCTTTCTTCCCTTTTTAAAATATTATTTCAAGCTTTGGCCTGAAATCAGTTCCTTCAATGGGACCTATATACAAGTGACCTAGGACATCTGTTTGCAACCTCCTATTCCAAGATATAATGTACATGACAGGGGGAAAGACTCACCTAACTACCTCAGGACCCAAATGCAAAATTTCCCAGACCTGACCTCTGACCTCTGGCACATACTCACACATTGCAGCCATTCTTAGAGTCCAAAGAATCCCCAAAGCTTGTACAGTGAAGTTTCCAGGAAGCCTGATAAACCACATTCCCCGGGAGGGAGGACGGCAGGCTAGGGTGCTCTTGCCCAGGTGGGCCTAACAGGACAAACAGAGCAACAAGGCCCAGAGCTGCCCACAAGCACTAGCTAGGGAATGCCAGAGCCACCAAACATGGTGATGAAGCAAGGCTTGTCAAAGCCATTTGGGGTCTTTGAAAAACACCGCCACAGGGAATGGAACTTTTGATTCCCTAAGATGGTGCTAGGCAGGTCTGGCAGGTCAGGGAGCAGTGGGTGGAGTCCTGGGCCCAAATTGGAGGCCCTTAGGGCACCCATTCCAGCATTCACTACTAAATTACCCCAGGCCCCAAGCCAGCATGGTTACATTTGAACTTTGCAGTGGAGAGCTTGGAGCTATGGTCTCCATCTGCCATCTAGCTCAGGGTCAGCCACCCATCACTGTCTCCAGCTTCATCCAGGGACTTCCCCTCAAACTCTCCCACCCACTTCCAACCCTAACACCTTCCTGGGGGTCTACAGGCCTCTAAACAGATCTGACCTACAGAGAAGCTCCTGTACAGCACCCAACTCTTCCGGGTCCCAAGTTAAACCCCATCCCAGCTCTCAGGGCAACAGCAGAAACACCAGCAGCCCGCTGAACACAAGCATAGCTCAAAGCATGACGCAGACAAGGCAAGTCCCACTGTCTGTATTTATCCAAGCCACTGCCTCCCCTCCAGGAGTGATGACTGAGGTTCTGTTTTAGACATAGGGCAGGGCTCTCAGCTTGCTCCCTGGAGACCAGCTCCGTGGACTGCCTGGCCTCTCCAAGTGTCCTTTCCAGCCAAGACCCTCAGTCACGGCCCAGCTTCCTCTGCCTGCCACACCAATTCACCAGAGATGTAAGTGGCCTGGACATGGAGAGTGTCGTCGAGCACCACAAAGTCTGCGGCAGAACCAGAGGATGAGAGGAGACGGTGGAATGGTCCATACCCTACTGCGGGGTCATTCATAACTCAGCCCAGGAAGAAAACTGAGGCCGTCTAGGAATGTAATCTGGACTGGTGGGGGAGCTGGAAGCAAGAGTATCAACTTGGCACAGTGAGGGTCCCTGTAACCCTCACATGGCCTGATGTTATATGCCAGAAAGCCAGGCCAAAAGCAAGTGTCGTAGCACATGCTTGTGATCCTACAAGGCAGGACTGCAGGCTCGTCAGGGCTATATAATAAGACCCCATCTCAAAAACAAAACAAACAAAACAGTAAGGGCCAGTCAGCAGATGACTGAGTCGGTAAAGGAGATGGGGAGGTATGGTTTGCCTCCAAGCCTGACTTAGTTTGAACCCTGGGACTCACATGGTAGGAGAGAACCAACTATAAGTTGTCTGTGCTGGGAGCAGCCCCAGACTCAGCCCCTGCATGGGAACTCACCTGCATCAGCACCAAAGTCCAGGCTCCCCTTGGTCTTCTCCAGCCCCAGCATCTGGGCAGGGTGGAGGGAGGCAGCTTCCAGGGCCGACTCCACACTACAGCCTGCTCAGGAACACACCATGGTGAGCATCCACAACCCTTCCCACGAGCAAGTCCAAGTTTCAGCTTTTAGGGACTAGGCTTGGGAATGGCCCAGGATTATGCAGCAGCTTAGGATGGTCCCTAACGCCCCTTAGCCGGAGGAGACATTGCTCATCCCTCCCTTCTCCAGCCCTGAAGGGAACCTATACTTTCAACAGACTAGACATGGGGCATCAGTTAGGGTGTATTCAGTATTGTATGATCATAGACCCTCTCGCCTACTTCTAATAAAAAAGCCATACCTGGGCCAACCAAGGCCAAATGGGACCCTGTATTCTTAGCAAGGCCTACCCCGAAGCCCAGTGACCACATTCCCCTTCCTGTATCACTTCTTGCCAGGCCATCCCCAGCCTATTTTCTCTAAATGCGACCCTGACGTTGCCCACATTCAGAGTGACACTCAACCTTGAGATGCTGCCAGGTATCTGGGAAGTCCCTGCAGAACCTGCTCAAAGTCCCAGGAAGCCAGTTTCATTCCACCCTGTCCATACTGCCCCTGCAGATTGCCATTATCCCACAGGTCTAGTCCTTGGCCTTTCCATGA
U5_15 24296134 24311184 15051 ATAGGGCTTAGCACCGCAGCCCAGCCCACTCTGATCAGGATGGCCACAGCCTTAGGAAAGAGTTAGCAGCACCTACTTGGCCCTTGGAGATGGCCCAGTATAATCATAACCCTCCTTTCAGACCCTCAGGCGACACCCTCTGTACTGGTTGGTTTTGTGTGTCAACTTGATACAAGCTGGATTTATCACAAGAAAAGGAGCTTCAGTTGAGGAAATGCCTCCTGAGATCCAGCTGTAAGGCATTTTTCTCAATTAATGATCAAGGGGGAAGGGCCCCTTGTGTGTGGGACCATCTCTGGGCTGCTTGTCTTGGGTTCTATAAGAAAGCAAGCTGAGCCGGGCGTGGTGGTGCACTCCTTTAATCCCAGCAGAGGCAGGCGGATTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAGATAGGAGGCACAGCACTGCTTTTAACTCTAGAGAAGCTTTGTGTAGTGGAGTGCATCTGTTGTCCCCATACAGGAGATCGTCTGAACCCAGGAGTTAGAGATCAGTCTACAGTAAAGCAAGACGCTCTCCCAAAATAAAAAGGGTGCAGGTCACTGGATCCTAGTAGTTTGGGGCTTGGAGACTTACTGCACTGGTAGAGCACCTATTTAGCATGTACAAGGCCCTGAGTGCAATCCCTCAGCACCATAGACGAAAGTTTAGGGTGTTTTTTTTTTGTTGTTTTGTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGAAACTCACTCTGTAGACCAGGCTGGACTTGAACTCAGAAATCCACCTGCCTCTGCCTCCCAAGTGCTGGATTAAAGGTGTGCACCACCACGCCCGGCGAAAGTTTAGTTTACGAGTGCTTGCATATGTTTGTAAAGCCACGTGCTTGCCTGGTTCCTGTAGAACCAGAGGGAGTGGCAAATTCCCTGAAACTGGAGTTACAGGCAGTTATGAGCCACATGATATGGGTGTTGGGAACCAAAGCTGAGTCCTTTGCAAGAGCAAGTGTTCTTAGCTGTTTGCCAGCCCCAAACACACATTTTCTTACCTTCACCTCTAATCCAAACCGTGGATCAGGAAAACAAAGGTATAGCTAGGAGTGGTGGCACAGGCCCTTAATCTCAGCACTCAGAAGGTAGAGACAAGCTGGCCTCTGTGGTTCTAGGGCAGCCTGGTCTGTACAGTGAGTTCCAGGATAGCCAGGGCTACCCAAAGAGACCCTATCTCAAAAACAAACAAACAGACAGACAGACAGACAGACAGAGAATAAAGACAAGGCTAAAAGGATGGCTGAGTGCTTAAGAGCATGTACTACTCTGACGCAGAGGACTGGATTCAGTTCTCAGTACTCACATATTCACATGCAGACATACAAACCTACATAGAATTAAAAATGATTAATCTTCAAAGAAGCAGCAGCAGCCGCCACCACCAGATGACTTAGTATCTTAAAAACCAAGCAAAGAGCCAGGCATGATATTATGTGAGAGGCTGAGACAAAGGGCCAGCCTCAATTACAAAAACAATCTGGACTCTTTGAGACCTTGACTCAAAGCAAATTAAAAAACAAACAAACAAACAAACAAACACCAGGCAAAATGGCTGAGTGGGTAAAACGCACTTGCCAACAAGCTATCTCATGACCTGAGTTTAATTCTCAGGATCCAAAGGATGGAAGGAGAGCTATGATGCCCACAAGTTGTCTTGACATCTGCTTGCATACCATGCACTGCATGCATGCAGGCCCACATAGGTTTTCGTGCTCACACACACACTCACTCACACACTCACATACACACAATTTTCTAGAAGAGCACTTTGAAAGGCAAAGTGAGAGCATGCAGCAGAAACGAGATAAATGAGGCTGGAGAGGAAGGTAATGATGAATCAGGGGTGGGCAGGGTGGCAGACATCCTGTCTGAGGTGCCTGACCTTCATCAGCTAACAGATCTGAAGCAGAAAATCATCTGAAGCCATCAGATCTGTCTATGTCTGAAGCATAGGCTAGAAGAGTAAATCTTAGAGAAAGAGCAGGTGGTTTCAATGAACCGGATCTTGATGAAGATACCCAAGTAGTAGCTGGGCAAGTGAGCAAGCCCCTCAGTGAGAAGGGCCCTGGAGGAATTAGGAGACATAGTAGGGAGGGTGTTTGAGGGTAGGTCTCCTGATGTCTAATGCAACGGGGTATTTCGTTTTTAAACTCAAGGCTGCCCAGTCTCAGCCATCACCTCCTACCTGCTTCCCAAGGCCTTTAGTCTAAAAGTGTTTTCTGAGAGTCTGTGAGGTAGCTGGACTACAGTGGGGCACCAGGGTGAAAAACCTTAGGACTATTCCTACTAAGGGAGAACCCCAGCCTTGCACCTTGCCAGTTGAGCCTGCTGCATGAGGAAAACACAGCGGTGGAGAAGGGGCGTGGGGGCAGGGCTCACAGGAGCAAGGCCTTCCGGGAAGCGCCCAGGAGGACCTGGCACACTTATAGCTGCTGGAATGTGCCTGAGGGGAAAGAGAAGCAGCTTAGCTTAGCAAGGGAATGGTAGTAGGGGGCACATCCTGACACCTGTCACCTTGATAGACGACTCTGGGTTTTGCCAGAGATCAGCTGGGAGAACCAAGTACCTTTCTGCAGTAGGACCATAGGTTGCCCTTGTAACCGTGGAGCACTGTGGGAGAGTTCCAGAAAGACATCCCTTGTTGACATTCAAGGAATGCTTGACATTGTCAACCACCCACTGACTGTTCTCACTCTCCATTTAATGAAACTGAGACTCAGAAGTAAGGTTGTCTTGAGTCTTCCAAGGGTGGCAAGTCAAGGGGCCAGAGTGACGCCTATGCAGCCCAGGGCACAGCCCACACTCAATACCCACAGTTCCCTCCTCTGCTTCTGTGTCTACACAACCAGAAAGGAAGCCACGGGCAACCACACACTCCCTGATCCTGTCCTGGAAGAAAAACTGGCTGCGGCGATTGACAACAGGAACAGAGAAAATCCATTCGAGTATGGATAACAGCATTCATTTACTAAATTCGTCAACTGTGTTGCATAAGAGAATACCACGTTTTTAAAGCAAGAGTTCAGGTCTAAGGGATAAACCCGCAGAGAACACATGAAGACAGCAACCTAAACAAGCTTCCTTAAAACTGGGAAAACTAGCCTGGCGTGGTGGCACACGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCAGATTTCTGAGTTTGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCAAGGTCTATACAGAGAAACCCTGTCTCGAAAAAACAAACAAACAAACAAAAACCGGGAAAACTGTGGGAGAACCTTACATGAAATTTGGAACAAGACAACAGACCTCCTGCTAAGATAGAAGGTGGAAGGGTGAGGACAGAAGCCAGTCCACAGCAACAGCAGCTCTTTCTGAAAGGCTTTTTCATGTGAGTCACACAGCATAAGCAGGTTAGGGAGGAAGAGCTAGACAAGAGACAATGGCTGTGGCAGAATTCCAGGCGACCTTAGTTCAGGAGACCCAGAGGCTGCCTACAGGGCGTTGGGAGGGCGTGGCAGATGAGAACCGGGGCAGGAAACGTGAGGACAACCGCACTGGCAGTTGCAGAGACCAGGCAGAACCCAACTGAAAGTGAGGCGGATCCCCCTCGGACCCAAGTCCAGGAAGCCCAGATTCGGGTGAAGGAAGTGGTCCTTTCCTGAGGGCCCCTTTGCTCAGGGGTAGAACGGCCTCATCTCACCTTGTGATAAACCTCTGGTGGGGAGGTGACCAGGGTGGGACAGAAGGAGGTGACACCGTGGGACAGGAGCCTCCGGGCCACTAGGGCGACCCCCGAACCCACGTCCTCCGTAGCCTTGGAGAAGTCAACACCAAATCCACCTGTGGCCACAGAAAACAAGGGGGGTAGCACAGTGCTCGGAGCTCCTGCCCTGGGTTCCCTGGCCCGAGCCAGGCCGCACCGTTGATCTGCACATCGATAAAGCCAGGCGCCAGGATGCGGCCTCCGCAGTCCCGCTGCTCATCAGCCACACGCCTCTCCTCAAAAAACAGCTTTTCTGGGTCCAGGATTCGGCCCCCGCGCACCCACAGATCCTCCCTGAGGGCAGAGTAGTAGGCACTTGGATCACCACCAGGCCCTGGAACCAAACCCTAGTTCTATCTCGCACCCAGCTTGTCCCTGCACCCACTATTCTTCTTGGTCCCGGCCACACACCCACGATAGTCCCGCCCTCCACATTCCTGCCCCTTTCAGGGCTTATGCCCTACACTATCCCCATCTCTGCACCCCGCACTGGCGTATCATCAGCTCTATACCCAGCCTAAGCCTGCACCATGCAGCCCAGACCTGAGGAGCGTGCCGCCACGCAGGATGCGGCAGTTGGTGAACTGGAGCACCGGGGCCCCCGCTGCGCACTGCCCGCTACGCATCGTGAGTCGCGCCGAACCCAGCAAGCTTTGGGCGCAAGCCGGCAGAGCCCGGAGCAGCCGTAATCGGAAGCGGGGCCAGGTCGCATCACGTGACTCTCAGCTGAAGGGGCTGTCACGTGGCCGCGGTGCTCACGTGGTGGCGACCACATGATGACAAACCCTGCCAGGCCCGCCCACAGAAGCGCCAGAACACAAGCCAGTCACATCCGAGAACTTTTATTGGACACAGCTGGGAGCCAGCTCTGAGGAGCAGTAGCACTCTATCCTCCACCCTGGCTGTTCAGGGCAGGGGGAAGGGTGGCTCCATCCAAGGAGTCTCAGCAACAGGAACCCCACAAGGCAACAGTCTACTAATGATTACTATATAGTTGGGAGACAGGAGCTGCTCCACAGACGCATGAATAGGTCAAATCTTTTATAAATAAACATCCAGTGAAGAGAAAAATGACAACTCTAGTTCATTCTTATACACAGGAGCACTCTAGGGCGATGGGTGGGGTAACCAGAGGCTGACTTCGTCACTGGTCAGGGGCCTGGGTGGGAGATGAGTGGGCCCGTATGTCCACAGCACGGTGCAGTAGGGGCAAGGCGGGGGCGACAGACAATGGGCACTAGGACACTGCACATTTACAAGACCAACTACTGAGGGGCAGCTGCCCGTGCATGTGTCAGGCTGTTCGTTCTGGAATGAGGAGGGGTGATCTTTACATCCTATCCTGTGACTGATGGTGGAAAAGGACTACTTTGTGGAGAGGATTAGGGCCACGATGAGACCGTAGAGGCCAAGCACCTCCGCAAAGATGAGGATCAGGATCATGCCCACGAACAGTCGAGGCTGCTGGGCAGTGCCCCGAACACCAGCATCTCCGACGATGCCAATGGCAAAGCCAGCAGCCAGGCCACTCAGCCCCACACTCAGGCCAGCACCCAGTTGAAGAAAACTCCTGGGGAGAGAAGAGACACAGGTTGTCAGCAATCTGCCTGTCCACAGGACAATGAAGCACTTCACCTGGGAGGAAAGGCCCAGCACTCACCTGTAGAGGGTGATGCCATCAGTCAGGGAGTTAGCGATAAGTACTGCCACCACCAGGCCGTAGATGGCGATGATCCCAGCCATAACCACTGGGATGATGGACTTCATGATCAGCTCTGGCCTCATGACTGACATGGCTGCGATGCCAGTGCCACTCTTGGCTGTGCCATAGGCGGCTCCCATGGCTGTGGGAGGAGAGAGTAAGTGAGCCCAGAGCAGCTTTTTCCATTCTGCTCCCCAGGTTCCCACCCATTACAAGAACTGAGGCGGGCAGCCAAGCAAGAGCAGCAGCCACTTCTCTTCCCACTAGCAGAGCCATACCTCCAACCTCCTGCTCTACTTCCGCCTGCAGTTCTTCCTGTGGTCATGGGGGCTCGTTTGCTGTGAAACCAGACTTTGTTCCCTAGCACTCCCCTTCCCTAGCTTGTGATCTCTGGCAACATAGTCACGTGGAAGAGGCGGCTGGGCCAAGGAGAGCCTTGCTCGAGACTCTGGCAATCCCTGTCCCACACCCTAAGCTCCAGGCACTTCCAGCATAAGAGCTACATGTCAGCTGCCCAGAGGCCACTCCAGTGTCAATCAGTTCCTGCCTAGAGTCTGCCTACAGTGGGGCCTTCAAGCCTTCCCAGACAGGACAACAGAGTTATATCTGTTTCTTCTGGTACTGCCCCCCCACTTCAACCTCTCCAAGAGAGACTGCTACCCTTCCGGACTTTGTTTCCCTCTCCTATCTTCAGGTCTTGAACTCCAATTCTTCTCATTTCTGCCACTTGTCCTCAGACCCTATAAGAAATATTGAGCTACTAATATGGCTGACACTGGAGCCTCCAAAATCTTTGCCCAGGGCACCCATGGCCATTGTGGGGGAAGTTGTGCATAACCCAATACACAGAAAACAACATGGGCCAGAGGTAAAAGAGACATGAACAGCTCCAGCCCCTCCTCTATATGACTTATGGATCTCACGGAACCACAAGATGTCTACTGTGTGCCCATCACTGAGTAGGGAAAGAGTCAGAGACCTCTGCCTTCTCAGAGCTCAGGAACAGCAAGAAGACAGTGACCCAACACAACAACATATTCAGCAAGGAAAGAGAAAAGGCCTAGGGGAGCTATGGTGAGGGGAGGAGGGTCTAAAAACAGATCACAGAAACCAACCAAGTATACGGGAAGGTGGTAAAGTGAGCAGCAGAGGCAAACCAAAGGGCAAGGTTCCTGACAGCACATTCAGACCCATGCACGACAGGACTAGGGGAGATCAACTGGAGGACTAGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTAGGGGAGATCAGCTGGAGGACTAGGGGAGATCAACTGGAGGACTAGGGGAGATCAACTGGAGGACTAGGGGAGATCAACTGGAGGACTAGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTAGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTAGGGGAGATCAACTGGAGGACTAGGGGAGATCAGCTGGAGGACTCGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTAGGGGAGATCAACTGGAGGACTAGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTCGGGGAGATCAACTGGAGGACTAGGGGAGATCAACTGGAGGCAGAACAGACAGCTTGTCTTTTCTACAGATTCTTAGAGGCCCAGACACTGTCCAACAAAGATCTGCATCTTCAATCCCTGAGTCCACTTCAAGAGATAGTTCCTGGAGCCATTCTGGTCACAGTGCATTTCTTGGGCCCTACTGAAAGCCAAGTCCATGCTGAGCTGTATCACGTAGTACAGAAAGTGTGTGTTCCAAGGGCTCACACTTCTGAGGCATGCTTGAAAACACCAAGAATACAGGTTGCCGTGCAGTACTTTAAAGGTGAGATCTCAACACAACTCTGTACCCCAAAAGGTGATACTCCAAGAAAGGTGAGTGAGTCAGGTCCTTCCCCAAAGTATAGATCATGGAGCTGAGGATGGGTGACAAGAAGATAGAGGATGGAAAACAGGGACACTGTCGGAGATCAGGGCAAAAGGAGGAAAAAAATTTAGCCCAGGCCCAGACAGTAGCAGCTACCCAGAAGAGCAAGCATGATAGGCTGCAGAAGGGAGACCTGCTGAGAATGCTAATTTAATACAGCATGGTCTGGAGGCACTTCCAGGAAATCAACAAATACAGGGAGGAAGTCACAGAAAATACAGAAAAGAAGTAACTGGCAGTTTCATGGAGAACATGATGAGGAAAGAACAGGATATTTGTCATAAGGGAAAAAAAGGGAAGTTAGATTGTTAGCATGAAACGGAGGCCTCAAGGATGCGTTAAAGAACAATGGTCTGAAAACTTGGTTAGTTAAGTCCAAGGGAGACCAAGGGTCAGTCACTGAGACGAAGCTTGGAATTCTCCCAGCAGCAACATCAGAGGCTGGAAGGATTTCTTTACGTAATGGGAGGACAAGACATTGTACAGAATAGCATGCCTAAAAGGAAAAAAAAGAAGTCAGAAGGTGATTCAGCTGGACAAGTAAGAGGCTAACCCCCACAGGACTGTGAGACGCTGGTGTGAAGGATCAGACAAGGAAAGCCGAGGTCAGCTTGCAATAGTGCAGACACACCTGTTGGCCAGCTTTAGCACATGCAAGGTCTTCATACAAGAGATCCTAGAAGCTGGGTGCAGAAAAAGAACTGTGGAGAAATTTGGAGTTGCAGGAGCCATCGTGACAACCAGAGCATGACACCCACATGGTCAGGGATATATTGCTGTGTTCCCTGAGACTCCTCCACTATCTGGGAAACAGTATGGACTCAAGAAAGATTTGCTGGATTAATTTATGAATGAGTGACAAAAATTAATCATAGGTGGCTAAAACAGGGGAACCTATGGACACATTATTCTGGGTGCAAAGAGCAGAAAAACATGCTTGGCAGCTTTCTCTAAGGAGACCTGAAGTGTGGGGAGAAGAGTCGTGGTGGGAGAGGAGGGAAGGAGAAGTCCAGGCCACATCTCCCACCACAAATGACCACTTACTCTAATGCTGTGGGTACAGCAGGGCTCTCTCAGCAGCACCTGACAGGTCCAGGCAGGGGAGGTCACCACTGACAGACAACCCTCTACCCCAATCTGCACCCTACAGGTGTTTACAGTCAACAGACACTGTTTGGAGTCCAAACTCACCAAGTACCTGCACAGAGAACCAGCCTCTCCATTTGCCAGGTAGGGTGGGGCAGGGCGAGGTCAGTTAAGCCAAAGACCAGACCTTCCAGGGGAGGCCCAGGCCAGACCAGTCCCCGGCCAGGGGCTGTGACTTACCTCCCTCAATCCCACATTCTTGGGTCTGGCCCAAAGCTTTTCTCCCAGGAAGGCCAATTTTCACTAAAATTTGACACTGAAGAATTGTCAAGGCCCAACTTCAAAAGCAAGATGCCATCAAGAACCTTGCTAGGCTTTTCCTTTTCTGGGCACGGAAGCGTCGCCCAGTTCCAGCCCCTCACCCCCCTAGAAGAGGAAGGTCATTCATTCACTCATCCATTCATTAGGTCCAGGGCTGAAGATCAAAGATGTAATCGTGTAAATGCAGGCACTCTAATGCCGCCCTCCCACCCACCTCAAAGCCCAGATCCCCAGCAGCCATCCACTAAAGTGCTCCTACCTAGGCCAAACCCACTTCCATAAGCTCTAACCGGAGTCCTTGCAGGTTCCCAGGACCAACCCCTCAGTCCAGTTTTCCTAACCAACCCACACCGATGACTGAACTATTGGGAAACCGAGACAAGCCTCAACCGGAAACACCTGCCTCACCCAGGGTAGAGACCCACCTGACCCCGGGCAGCTGGGCCGCCATAAAAGCAGGGCTTGTGAACCTTAGCGGGGACCCAGGCTCCCTCCAGCCAAGAGGCCAGGGACTCCCGTCCTGGGCCTGCCCCCGCCTACGGACTGGCGCCTTTCAAGATGTGATGTCACACCTGCCTGCAGAATGCGAGGGGGCACAGCATCGCGCGCCGCCCCCCGCGCCCCCCACCCCACCCTCATTCCTACTTCCCAGGACTAAGATGGCAGCCACACTGCCGCCCCACCCCCACAGAAGCGAGGCACAGGAGGCGGGCTGATAAGACCCGCAGCCGGATCAAGCCCCGTGCTCCACAAATCCATCAGTCCCCACCACCCCCCCAAACCTGCGCCCGTAAGGCCCGACACCCACATAGCTGGGAATGACGTCAGAGTGACGTCACGCCCAACCCCCAGTGAGCAGCGGTCTAGAGTCCCGCATCGCTTCGGCATCCCCTGCTCCTCCTGCAGCGATGCTCACCGCTGAAGACCATGGCGGACGAGGCGCCCATGACACCGAAAAACGAAGAATATTCGGGGTTGTTCTTGATGTCAGCCATGTCTGCAAGCAGGGAAAAGACAAGCTCAGCGGGCGGCGGCGAGCTAGGACAACGGGACAGCGAGCGAGGAGGCAAGCGAAGATCGTCGAGGCAGCGGATGGGAAGGCGACCCCGCCAGTCAGGCGCTGCGCTCTAAATACCAGCACCGCAAAGCAAGATGGCGGCCGCGCTCGCGTCACATGATCTGTCCCCGCCCCCACGGGCTGTACCACTTTGCGCCGGTGGGGGAGCGAGGCTCCAGGCTCGCCGGCAGCCCTTGGACAATTTATGGGCCCTGGCACCAGTCCTCGGGGCCCGGGGCCCAACGCTCAGCCTTGCCTGGGTCTTCGCTGGCCCGCGGCTCCACGCAGTCGTCTTCTCGAACCCTACGGGTTTTGGGATCATCGGTCCCCCGGGGCGGAGCCGTAGCGCTCATATTAGCATACAGGGTGGAGCGAACGCTAGCCCACCTTCACTGCGGCTGCGCAGAACTCACCGGTGTTCGAGTACCCGTGTTCGTTTTCCCTGTGTTCACTGCTCACGGACCTCAGGCACTCCTGCTGCATAAGACTCAGGTTAAAGTAGTGACATCTAGCAGCACTACAGAACTTATAACAGAAGAAATCCCTCGTGTTGGGCTTCGCTGTCTCCGCCAACCCCCAGGAATACCACGCCCCACCTCTCCGGGCTTTAAAGTGCAACAGCCCTAGGTGTTCTGTAGTCACCTTCTTCCATACCTAGGCTCCTGCAGATTCAGGCCCACCGTATTCCCTTTAATTCCGGTGGTGGTTGTGGGTCCCTCACCTGACCCCCTCTTGTATAGCTGTCTGCCCACAAAGACGTAGGCATGGCTGTTTTTCTATTTATTTTTCTACTTTATTTTTGAAACGGGGTTTCACTGTGAAGTTCTGGTTAAGCTGGAATTTGCCATGTAGACCAGTTCCGTCTTGTCTGCCTCTTGAGTGCAGGGGGTTAAAAGCACCCTGCTTGATGGGCTTTCAGCATTGACACCTGTGAAAGGTAACCCAGATTTCTGAAAGTCTCAGAAAGACCTGATTGCCCTTCTAGCACCCATGCAAGGCCTACCTGTGCCAGGCCTCCTTTCTAGACCACTGATGGAGGACTGGTTTCCCCAGCAATGGAGTGGCTAAGGCTACCTCTGCCCTCCAGATACCCTTGTAGCAGCTTACGCACCCCAGCTGCCTGCCTTCCTTCTACCTATCAACTCAGGCTGCCTAGTTTCCTTTACCCTTCCCTCCTGAGCAGCCACGACCTAGCCTAGACCTCCCTTGAAACTCAAGGTCACATTCTAAGACCAGTGGGTTCTCCAAGTGGTCTCAATGCTAATACCTCAACTTCTCCATGCAGGCAATAGAGACACCTTCCCATCCTGCTACCTTCTCAGTCCTACATTTCACAGCAGAAAACTCCCTGTGTCCCCTTCCCACCTTGGCTGAATCCAGTCTACCACCCTTTAAACTGGCCCGTAGAAGAGCCTTTGTCTTGTTTCAGAGAGGACGTTACTGAGAGCAGCTTTAGCCCTTCCTCCACCCTCTCTTGCTCCATTCTGACTTTTGAAAAGAGGAATCTTCACTCTTGCCCCCACGCCTTCACCCTGTCTCTCTCCAACTCCTTGCCCTTGCCAAGGTCACACTTCCTTTCTAAGCCAGCACACAGGGCCTCGGTACTGCTATCTCCAGTGCTTCCTCCCTCTCCCCCTCTCTTTCTCCTGTGTGCCTAGAGCCTGAAGAAGTCAGAAGAGCTCGTCAGATCCCCTAGAGCTAGAGTTAGTAATGCCTGTGAGATGCCAGGTAGGTGCTGGGAATTGGACAGAAGTCCTCTACAAGAGCAGTGAGTGTTCTTAACCACAGAACCTTGCTTTAGTCCCCATGGGTTTTTTGTTGTTGTTGTTGGTGGTGGTGGTGGTGGTTTTGGTTTTTGGTTTTTCGAGACAGGGTTTCTGTGTGTAGACCAGGCTCGCCTGGAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATTACCATGCCCGGCCCTTGGTTTGTTTTCTATTCAGCACTTAATATCCCACAATTCTTTGCTGCTTAATCATGTGCTAGGTAGGTCCTTCTTGGTGCGCTTCCCTTTGCTGTCTGCTATCCTCCAGCACCAGTTCCCAGGTCTCCTTACTTACTACTCAGAACCCATTTTCCCACAGGAAGTCCTCCTGACATCCCCCATGTTTCTCCTCTCCCTCCCCTAAGTGTCCCCCTCTGGCCCTTGCATCGAAGGTTGTTGTGACTTTTTTTTTTTTTCAGACATCCAGTAGGGTGCCCAGATCTGGCCACCCTAGTAACAGAGCTAAGCACCTAACTATATGTCAGGGCCTATGCTGAGGCATTTGAGGTTATTGTCATGCCTACTCTTAGTCACTGGTTAGTGAGACAAACCTAGGAAGACTAAGAGCCTTCCTCCAGTCACCAGCTAACAAATGAAGGAATTACAATTCTGGTCTAGGGCCCGCGCTAGTGGCTGAACTACCAGGTGAGCCACCATCTCTCACTTCGTTCCATTTCAAACCTCCCACCCAGATCTTCCAGGCACTGTGCAGGGCCTCTACTCTGAGTGGACTCTAGGAAAGAAACCACAAGAGTGCATCCCTGCCTTCTCCTTTACTGTTACTTCCCCCTTGGCATGAAATCATAGGTCATGAGTTCTGGCCTGTCACTGTCAGTGGGGATTTCCAGACACAGAGAGGGAGGGGCTTGGAAATGCACATCAGAGACCCATAAGGAAAGGGACAGAGATGGAAGCTGGGCTCCTTAAACAGCCGATGGCACTTTCCAAAGGTCCTAGAGAGGAGGCAGAGAAGTTCAGACACTTCTTAACATCCCAAACAGCCGTAAACTGGAAAGACCAGGGAAGCTATTCCCAAGAAGGAAAGACCAGGCTTGAGAGTACACAGCTGTCACCTCAGCACCATGGGCTCTGAGGCAGAAAGACTGTTAAGTTCGAGGCCAACCTGGGCTACATTGTACTGTCTCCAAACAACAACAACAAAAAAAAAGGGGGTGAGACAAAAGAAAAGAGAAAAAATGGGAGGAACAAGGGGAGGGGGAAGGGAAGTAGACAGGGAGGGAGAAGCAGTAGCCTTTACAGGAACATCAAGAACCGTGTGACCCAGGAAAAGCCCTGCAGCAGCCTCCTTCCAACCCACCACAAAGGCATGGGGGTGGAGAGAGAGGAACCTGGGTGAGATAGAACCACGTGTGGCCCAGACATGGCTGGTGGAAACATTAAATAGCATAGGTAACGGTACTATTCTGAAAAATGGAACATGATCAAAGGCAGCTTGTTCTTTTTTTTTTTTTGGTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCATGCACCACCACTGCCCGGCGGCAGCTTGTTCTTTAAAAAAAAAAAAAATTAAACTAAAAACTAGTTGAGCCATGTATGGCAGTGGACATCTGTAATCCCAGTGTTTAAGAGGCTGAACAGGATGGGAAATCTTGAGGCTAGCCTGGGCTATACAGACATAAGGGTGGCACTGTAGAGTGTGTGTTTAGCAGCGTGCATAGGGTCCTGGATTTAATTCTTAGCACCAGAAATAAATACCTATAGACTACGGATCAAAGAAATAACAACAGGGAGGGCTGGAGAGAAGGCTCAACGGTTAAGAGCACTTGCTGCCCTTGAAGAAGACCTGGGTTTTAGTCCCAGTATCCATATGACAGCTCATGGCTGTCTTGTAACTTGTTATAGGGAGTCTAACGCCATCCTCTGGTCCCATGGCACACGGGCATATGTGCAGGTGATCACCCACAGAAATAAAAACCCCTGCTGCTCGTGGTGGCACACTCTTTAGCCTTAGCACTCAGGACTCAGAAGTAAAAGGATCTCTAGGAGTTCAAGGTCAGCCTGGTCTACATTGCAAGTTCCAGGAGAGCCAAAGCTACATATGGAGACCTTGTCTAAAATAAAATAAAAACTAATAAAGTCCAATAAACACAGAATCTGTCTTGCTCTGCTTGGGCTCCTCGATAAGTTTGAATATCACATGGTTTGCAAATCTACACTGTTTCTATGTATCAGACCACAAGAGAGACACTAGATACATATTTACAAGGCAATGGTTCTAGAGACAGAGAAACCCTTCCTTCTAGCAAAAATTCCAAACTTCAAGAGTGCAAGAGTGATTGGTTCTTAACGCTGAAGATAAACAGGCAGGCAGTGAGGCAGCTCTGCCACTAGGGGGCATAGGAGATTAGTTTACTGTAACCTTGGCTCTGGAAGCTGGTTGTCAGGTGTTGAATAAACCCCAATCTGTCTTTGACCCTATGTTTCTCCACTTGGTAGTGGGAACACACTATCAGGAAAGCAATGCTGAGCTGGGAGCCTGTTGTGGGAGCACACGCGACCCTGGACAAATCTCCCAAAGGAAGCCAGAAATGGGTTTGCTAGATAAAGGGATGTGTGCCCAGAAGGAGGATCTGGGGCTCCGTCATGAGGACCCAGAAAAAGCTGATCCCTAAATTAAGTCTTAAAAGATGAAAAGGTTGCAGGGTGTGGTGGCGCATGCCTTTAATTCCATGCATGGTAGTAAAGACAGATGGATCCCTGAGTTCAAGGCCAGCCTGGTCAACAGAGTGAGTTCAGGACAGCCAGGGCTATACAAAGAAACCCTGTCTTGAAAAACAAAAAAGAAAAGAAAAAAAGATGAAAATGTTTAAAACAGGAAAAAGATGCAACCCAGTTTTCTTCTTTGTGTACATGTGTGGGAGGCTTAATGTAGCCTAGGCTGGCCTTGAACTCCTGATCCTCTGGCCTCTTAACTCCCAAGTCCTGAGCTCACAGGTGTGCTCCTTGTGTTTGTC
D3_15 24279174 24294222 15049 TGTTTTTTTAAATTATGGTGAAACGCATACAATTTTTGTAAACTTTTTGCGCTGTGTGTACAGTGTATGTGTGTGTCCACTTTGTGGGTTTGTCAGATGTCTTCCTATACCAGTCGCTACCTGGAACTTGCTGATCCCTCTTGGCTGGCTGGCTGGCCAGTGAGATGCAGTGGTCCCCCTGTCTCTGTTTTCCCAGGGGTGAAGGTACAGACACACCCCCACCATCTCCAGCTTTCTTTTACAGGAGTGCTGAGGACTTGCCTGCATAGCAGGCATCATACTGAGCCATCTCTACAGACCTTCCCCATTTTTTTTTCAGTGTTGGGAATTAACTCAAGACCTCACACATGCTAGATCATCTCAAAATTAAATGTTTCTAAATTACACCCATAGCCCTTGCGCCCTTTGTTTTTTGTGGTGTTTTTGAGACAGTGTCTCAGTATGTAACCCTGGCTAGCCTGGACATCACTATGTAGACTGGGCTTGTCTTGAATTCATGATCTTTCTGCCTTAGATTTCCTAGTGCTTGGGTATAGGTATTCACCACCCTTTCCAGATATTTGTCATTTGTGTAAAATAATTTAAAGTGCTGAAAATTACTGTGAGTATTCAGCCTTAAATGGGGCATCTAGAACCAGGTCATAGTGGCTTATTCCTTTGATCTCAGCACTCAGGAGGCAGAGGCAGGGCTACACAGAGAAACTCTGTCTTGCAAAACAAACAAACACAAGATAAATAAAAGGGAGCATCTATATGAGTCCCCCTCCAAATAAGGCTTAGGGAACCTTGGGAATGTAAGAGGAGCAATGCTGAGAAATGCTGACTCTGACCCTGATGTGGCCTGGCACCGAGGGTCTCACCACTGCTGTGCTCACCTGCATAGGATCAAGCCAGTCAAAGTTCCAGCACCGACAGGAGGGGGCCTCACCAGGCTTGCACCTGTCTCCATGGTTACTGGCAGCTGATGGCCATTAGGGAAAAGCCATTCTTCCTTGAGGTGTAGCCACTAGAAGGTTGACCATGCTCAAGAGGACAACAACACACACATGCACACAGACAGCACTAACCAGACTCAGGAGGTTGCTAAAAGAAACTACCTGCCTCACTCCAAAGAGTTTAAATCCAGATGAAGTGGAGTGAACCTATGGAGTCATATAAAGTGGAAAGGAGAATAACTTGAGTTCAAGGCCAACCTGTGCAATATAGCAAGATTTCTCAAAAGAAATGTAGATAATATGGAGCAGCCAACACTCAGGGAGTAAATTCATAATCCCATGTAGGCAGAATGAACTGCTAAACCAGCAGTAACGGCCACTAACAACTTCATATTGACCTCATCTTGTCGCAAAGTTAGCAATTCCATCCTACATTGCATTTGTTCTAAAGCACCTCTGACTTTGTTGCCCCAGTTTTCAGATGAGGAAATTAAGGCATAGCAATGGCAGTTCTAGAGTTTGTAGTGCTACGACCGGGACCAGCCTTGGTCCACATTCTAGAACCTGTCTGTCAACCCCTAAGGATACAGCCCATAGCTATCCATTCTCCTAGTCTGCCATACATTTATAGACCTTAGAAACGGGGGCTGGAGGGATGGCTCAGCGGTTTAAGAGCATTGGCTGCTCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACGACCATCTGTAATGGGATCTGATACCCTCTTCTGGTGTTCCTGAAGACAGTGAGAGTGTACTCACATACATAAAATAAATAAATCTTAAAAAAAAAAAATGCCGGGTGGTGGTGGCACACACGTTTGATCCCAGCACTTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAATGAGTTCCAGGAAAGCCAGGGCTATACAGAGAAACCCTGTCTTGAAAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAATTCAGCCCGGGAGATGGCTCAGTGTGTGAAGTGCTACAAAGGCATGAAACCCTGAGTCTGGATCCTCATAACGAGTGTAAAGCCAAGCACAATTAAGCTCATCTGTAACCAGTATTCCTATGACAAGCCAGTTATCCCAGATCCTGAGCAACAGATGTGACATGCACGGCTCAGCAAGCAAAATACTTGCTGTAAGCCTAAAAACCTGAGTTTCACCCAACCTAAGTAAACAAGGGTGAGAGCTAACTCCTGAAAGTTGTTTGACCTCCATACATGTGCCAAGGTACACACAATATAAGTTTCCTAATATTTTTATTAAATTTAGTTTTGTATGTATTTTTCTTGCATGTTTATCTGTGCACCGCATGTGTACTTGGTGCCCACAGAGGCCAGAAGAGGGCATCAAACCCTCTGGGACTGGAGAAGGTGGTGAATCAACGTGTGGATGTCCTGCAAGAAGAGCAACAAGTGCTCTTAACCTATGAGCCATTTCTTCAGGCTTATAAGTTTCTATGAGTTCAAGGCCAGCCAAGGTTATACAGTAAAACCCTATCTAAAAACTCAGAACAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAGAAACCAACCTGTGTCAAACAAGGTGGACGATAAGGACCCTATCTCAAAACTCAGAACAATAGAGAGAGAGAGAGAGAGTGAGAAAGAGAGAGAAAAAGAGAAAGAAAGAGAGAGAGAGAGAGAGAGAGAAAGAAAGAAACAAACAAACCTGTGTCAAACAAGGTGGACGATAAGGACCAACATTGGAGAGGCTGCTCTTTGACCTGCATATCTGTGCCTTCGCTCACACACACAAACATGCACACACTTCATCTCACATGAATGCACACTCCCATGGGGGAGGGGGTATTGATTTAAAACAACAAGTGACCATAACCCCTAATATCCAGAAGTCTCGTGCTACCCATGCTGGTTTGATCCATCCTTCCATTGCTCTGAACACTGTATCATTCTGTGCCCCACATGGCTCTCCTGATGACCTACCTGCCTCAGACTCTGTTGAAGAAACCTTCCATTTGAGGTGGGTTGTTTATAGATCCACATAGTGACAGATCTCTAACATGCAAAACATTAAAATGGTTCCCAGTTTATTTTTGTTGTTGTTCTTATGAACCAGGTCTGCACCAAAATCCAGACACACCTAACTTGCTTTCTTGGATAAATTTGTAGCATATGAAATTAATGGGTCAAAGTGCAGTTCATTGCTGCTAAATTGCGTGCCCTGTTCAACAAAGAGCATCCCATGTCTCGTCCTTAGAACACACAAGGGCAAGCTGGATGTTGATGGCACTCTTGCCAGCACACACCTTTGAAGACTTTGTCATCGTTATCCTGAATGGTTTACCTGCCATGCAGTGAAAACAATGTTAGCTTTCACCACTTGATGGCCCCAGGAGAAGACCACCCATGTAAGGAGACTAAGGCTGCAGTGAGCAATCACACTCTCAGAGTTAGATAAGTCACAGTACATAAGTGCCCAACTAGTAACCAACTCTGCTCAACTGCTTCCCAATCCTTTCAATTTTATTTGGGTCTTGCTTATCTTGAGAAAACACTAACCTTTTTTTTGTTTTGTTTTGGTTTTTTTGTTTTGTTTTTCCAGACAGGGTTTCTCTGTATAGCACTAGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTTGAACTCAGAAATTTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGTGTGCGCCACCACGCAGGGCAACACTAACCATTTTATATGATTAATCTTCTTTAGTTCCCCATACAGCCTCCTGGGATGCATGGTTTATATTCATTATCACTTTTTTTCTCTGAGACAGGGTTTATCTGTGTAGCCCTGGAAGTCCTGAAACTCCCTCTGTAGACTTCTATAGGCCAGACTGGCCTTGAACTCAGAGATCTGCCTGCCTCTGCCTCCTGAGTGCTGAGATTTTCATTGCCATTTTACACATAAAATAATTTGCCAGAGTCTATGGTAACCACTGAGTACTGATAGCTAAATACAGATACTCTGGTTGTGAGAGAGAAACAGAGAGAGAGAGAGACAGACAGACAGAGAGACAGAGAGAGACAGAGACAGAGAGACAGAGACAGAGAGACCCTTTCTCAGAAAGTTACTGAAGGATGTGTTCCACCAAAAATAAACAAATAAAGGGGCAAACCAAGAAAGAAGACATGAGAACTAGAGGAAACAAGAGGAAACAGAATGCACAGGAACGGAGTGTAGGGAGGTCCCAGGTCCCTGGTCCTGCGTGTCCATTCAGCAGTCACTGAGCCTCCCCTGTTGCAAGCACTGTGCTTGGTAATTCACCAGTGAAAAAGCACAGAAAGCTCTCCTGTGGAATTGAGACTAATCAGGACTGGGGGAGAGAGTTAGCCGAATAAGCAAATGATACAGCATATTAGAAGGTGGTAAGTCCTGTGAGGAAGAGGTCTAAAGGGACAAGTGCCAAGATCAGAGCTGTAGGCAGGAAGCACCCAGACCCTAAGCAAGCAGGCTTCCAGAGTACTCCAGAAAAATCCTTAGATGATTCTCATATTTCCTATAGAGAGTTGAGCTGAGAAGCTGATATAAAGAAAACACCAGTAAAATGAAACAAAGCAAACAAACAAACAAACAAAAAAACCCTCTACTAGAAAGATTACACATAGAAGGACAGTGGTGGCGCACGCCTTTAATCCCAGCACTTAGGAGGCAGAGGCAGGCAGATTTCTGACTTCGAGGCCAGCCTGGTCTATAGAGTGAGTTCCAAGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAGAAAAAAAAAAGAAAAAAAAAAGACTGTAATAAAATCAGTATCCTCCCTGTCTTCTTGCTTTTCATGTCCACCTTCCTAGGTGGTATCTTTATATCGAGAGTCATCATTTGTGGTTAAATATTGCTTTTCTGCTGAATATCCTGCCACAGTTCCAGCCTCATGTCAAGAGAACCTGATGATTCAGGGTTGAGTCAGTACCTGATCATGTCTGTAAATACCTCTCACAGCAGGAGAGGCACTGAAAAAGAGGCTCAAAATATGGATGTGTAATCTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTCTCCTGAGTGCTAGGATTAAAGGTGTGCACAACCATGCCTGGCCTATTGCAATCTTTTTAGAACTATTTGTTTTTGTTTTGTTTCTTAACTTTATGTGTCTAGGTGTTTTGCCTGAATGTATGTATGAGTGGTATGTATGAGTGTTATATGTGTGCAGTGCCCAGGGAGGTTAATGATGGCTGTAAGCTGCCCTGTGGGTGCTGGGAATTGAACCTGGTCCTCGAGAAGAGCACAGTGCTCTTAATGCTGAGCCACCTCTCCAGGCCCACGATTTCCACTTCTAATTACTGATGCAACTCAAAATTGATAAAATGGAGCTGGGCATGGCCAACTAACTTCTGTAAATCCGGTATTCAAGACACTGAGGCAGGAAATCATCAGCCACACTTCAGAACTCTCTCTCATATATATAAAACTGGGAAGAATATTTGTGACCCCATCGTTTACTGTTGGCCTGTGTTGCTAAGGATTATGCCCACATTCTCACTTCTTCCAGGTAAATGATCTATCACTGAGCAACATCCTTAGTCCTGGCAGCACAGAGGAGAGAAAAAGACCGTGAACTCAAAGAACAAGGGGTACAAGGGGTACATGGAGGGCTTAGAGAAGGAAGAGGGAGATGATGCATCTATGTTCTATGTTAAGATCATATATATACATACATATATATGTGTGTGTGTATGTGTGTGTGTGTACATACATACATACATACATACATACATACCACCTGGCGAGATATATCTCGCCAAGTGGTACACTCAGGAGTCAGAGGCAGGCAGATGCCTGAGTTGAAGGCCAGCCTAGTCTACAGCATGAGTTCTGGGACAGCCAGGGCTACACAGAGAAATCCTGTCTTGGAAAAAGAAGAAGGAAGAAGGAGGAAGAGGAAGAGGAGAAACAAGATAATATAAAAAAAAGAATGAAGAATCAATGTCTTGATTGTAAAATAAATGAGGGCTGAGGATACAGTTCAGCAGTAGAGTGCTTCTCTAGTATGTGTACTAAGGTTAATCCTTGGTACCGGGCTGGCGAGATGGCTCAGAAGCTAAGAGCACTGACTGCTCTTCCCAAAGTCCTGAGTTCAAATCCCAACAACCACATGGTGGCTCACAAACACCCATAATGAGATCTGATGCCCCCTTCTGGGTGCATCTGAAGATAGCTACAGTGTACTTATGTAAAATAATAAATAAATCTTTGAGCCAGAGCGAGCAGGGACTGAGCGATCAGGGCTGACCAGAGCGAGCAGAGGTTCCAAAAATTCAATTCCCAACAACCATACGAAGGCTCACAACCATCTGTACCGCTACAGTGTACTCATATACATAAAATAAATAAATCTTTTTAAAAAATCATTGTACTACTAAACTAAAAAGATAAAACAAGGAAGAGGAAATGACTCAGGGATAGGAACATTACCTCATAGGCATGAACCCACTTAGAAGCTAGGTAGGTACTCAGTCAACACTCAAGAGGTAAAGACAGAAGATTCTTGGAGCTAGCTAGCTATACTAGCCTATTTAATAATCTCTAAATTCATTTTAAAGAGCCTGCCTAAATAGAATCAGGAACTCAAAGAAGATACCCAGTGTCAACTTTAGGTCTCCACATGTATGTACATACACATGTAAATGCATACATACACACATATAAACAACCTCCTGTTGATCCTGGACATTTATATGCACTTACAAGAAACAGATCAAAGTTCTAACAGAGAAAGAGTAAAGTCGGAGAAGCCCGCCATGCCAGGTGTGGAGGAGGCACATGCATTTAAACCAACACCTGGGAAGTGGAGGCAGGCAGATCTGTCAAACTCAGGCCAGCCTGGTCTATGAAGTGAGTGACCAGTCACCGTGACCAGTCAGGGTCACACAGTGAAACCCTGTTTCAAAAAGTCAAACCCAGGTTTTTGTTTTTGTTTTTTTCAAAATAATAAAAAAGAAGCAAGTTAATAAATTTAAATCCAACAATGGCTAGCAAATGGCTCTGCAGGTAAATGTACTTGCTGCCAAGCTTGGTGACCTGCCTGAGTTCAGTGCCTGGGACCTAATTGTCCTCTAACCACCACATACAGGCTGCGGCACACGTGCCACACATACATACATAACTACATGTAAAATACTTTTTAAAGATCCACAGAAAGTGAGGCGTGTTGCTGGGAAAAACTGCTCGGAGACAGAGTGCTTGGCCGAGTTGAATGAGGCCCAAGGTTCATTCTCTAGCATGGCAAAAGTGAGATTCGGTGGCACGCCTCTAGCTTTAACACACTGATGAGTAAGGTAGGAGGATCTCAAGTTCAAGGCCAACCTTGGTCACAGAGTGAAACCCTGCCAAAGGGGAGAAAAAATACAGTGGAGATTTTTCCTCCAATACAGGCGTGAAGGACAGGCAAGATGGCTGAAGTAGTGTGAAGGCACCTGCTGATCATGTTGACCTTGAGTTATGTCACCCAGACACCTCACAAGGGAACCTACGGCTACTTCTCACACCAAGGCACTTCTCTTTTTAAGGTAGAAATTTAATATCCAGCCTTGATCAGCCTAGAACTCATTATGTATGCCAGGCTAGGCTTAAACTCATAGAGATCTTCCTGAATACTAGGATTAAAGGCCTGTAAGACCTGGTATGTATATATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATGTATTTAAAGATCTTTTTTAATGTTATGTGAGTACAGTGTCACTGTCTTCAGACATGCCAGAAGAAGGCATCAGATTCCATTACAGGTGGTTGTGAGTCACCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAGAGCAGTCGATGCTCTTAACCGCTGAGCCATCTCTCCAGCCCAAAGCCTGTTTTTCAAAAGTTGTGGTTTTTTTGGTTTTAGTTTATATAAAGTTTTTATGCACAGTGTGATGTTGGGAATCAAACTTAGGAACTTGAACAGAGTTAGGCACTCGATCTACCTCTACAAGTTCAGTTCCCAGTAAATTGATATAAAGTTAAAATCAAAATGAAAGCCTGAGGCACCAGGAAATGGTTTAGTTGGAAAAAATGCTTCCTTTGCAAACACGAGAACCTAAGTTAATTCCCGAGTCCATCTTGTGGAGGACCCAAGTTCAGTTCCCAGCACCCAATCAGACAGCTCACAACTGTCTGTAGCTCTACCTCTAGGGAGATCCAACACCTCTGGCCTATACCTGTACTCATGTGCACATACTCCACAAACACGTGTACTACCCCCCCACACACACACAGACATACCTCTCCTGCCACCCCCACACACAAATGTTTTAAAGGTAACCGTGCAGGTGACTAGAGCAGAGGAAGGAAGCAGAAGGCTGGAGGGCGATTCTGTCTCCAGGATCTTCATGCAGGGAAAGACCTGAGCTAGCCTTAACCAGGTAACTCCTCTTACCTCTGTAAAGACAGAGCACCTGTGTGGGAAGAGTCTGGCTGCCCACCTCAGGGCAACGAACAGGAGGGGAGACGGTCTTACCTGGCCTGGTTCAGCTCACTGGTACCCAAGTTGAGGGAGGGGGACTGTCTTTCCCTTCCCAGAATCCCTTGGGTCTACAGAGTATCTTGAAGCTAAGTATGAAGTAGCTTGAGGGGAACATTGTGAGGTCATGGGAGGGCAAGAGGATGATGGGAACCATCTTAGAAGGGGCTGGAAGATGTCTGGTGTGCACACAACACTAGCTGCACATGGTTCACCCTGTTTCTCTGTCCTAACTAATCCTCATGTAGGAGTGAGGAAACTGGGGTTCAGAGATTAGAAACTTCTACCGATGTGGCACATCTGGACTGTTAAATACTTTCTGCTATGTAACAAATTACCCCCAAAACATTACCTCAAGATGTTTTGGAGTTAGGAGCACAAAAGTGGCCCAGACCTACAGTTAGGCTCAGGTTTCTGCCGGCTGTGTCAAGGTGCAGCGACAAATGAGTGTAGTAGCCTGTGTCTTCAAATCCAGAGGCAGAAGGATCCCAGTTCAAGGATAGTCTCAGATACACAGCAAGCTTAAGTCTGTGCTGTGCTAAATAAGGCTCTGTCCCCCATCCCCCAATCAAAAAAAAAAAAAAGCAAACGTCCCAGTCGTTGAGAGGTCTAAAAGTTTGTTGCTACAGAGATGGGCTGTAGACCTCAGCTCCTCCCACGGAGATGTCTGCCTGCCAGGTCTGCTGCATCTCCCCAGACAAGCGGCCCCACCTGGAAAACTCTCCACTTAAAGTGTGTTCTTTTTTACAAAAACCATTAAAAGTAAGTTGCAATCTGGGTCATTAAAACAAGCACGACACCCTGGCTATTATCATGGGGAAAAGACAGGTCCCTGTGGAGCCCCTCGTGGTACACTTTCCTTTTTTTTTTTTTTTTTTTTTGGTTTTTCGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCTTGGAACTCATGGTACCCTTTCCTTACAAATGCCACCCGGGCACTCCAGCTGGCCGGCCAAGGGACCCTGAAATGAAGGAAGGATTCAGGGGTACCGCAGGTCCTGCTTGATGCTACAGGCGTGCTGACTCAAGCTTACAAATGGACTCTGCCTATGTTTGTTCCTTTAGCTAAGTCCAAGGGTTTCACCGGTCCCACAGCTCTGTGGGACCGCCTTGGACCGCGGTTGTTGTGATTGGACCTTGGGAGGGGGAGTGGGGGAGCCTCCGGCGGTGGGCGTGACCACCGTGGAACCCTGCTGTACCCACCCGCTGGGGTGAGCCACTCCTCCATCCTGCTGGAGCCTGCCTGGGTGGTTAATGTCGCTGGCCACACCCGACTCCAAACTAGGAGTAATTTCTGCCACGGATGACCTGGACCCCTGCTCAGGAATCATCAGCGGGTCTTAGGAACACCATGTTGCCATGACACTGGAGGTAAAAGGACACGGTTGGGTCCTTTGGCGCGCGGGTCTCAAACCTGAGTTCAACTGAACCTCGCAGGCTGCTACACAGCGCCCGCCGCCAGATGGCACCCATACTTTCTGCAGTACCCAAGCCTTTTCCTCCCGGACACACTGGGTATAACTGTCCGCCTCCACCAGGTGTCTTGGGAGCACTTGTACTGCTCCCCAACTTGTTCTGCACACTACCACTACAGTGACTCAGCTAACCTCAGGGAGTGGCTGTCCCCCAATCCACACCCATACCACAGGCACGTCTGATGCCGTACCAAAATCACCTAAGGCTGGGGCGGCAACTGTGCCCCAGAAACCCCAGGCTGAAGTAATTGGCACAACTTTCTGTGAATAGCCCTCCAGCTCTGAGCCATCAACAAGGGCAAGCTTGTGATTGCAGTAGGGGACTAATGCCAGTGACAGAGTATGTTTGGAAAGTAAAAGTTCTGGAGATAATGGTGTGGTGAATGCATATAGCTACAGAACGTTACAATTAAGAGTGGTTAAAGTGGGGCTGGAGATGGCGCAGTGGTTAAGAGTACTTGTTGTCTACTCTTCCAGAGGACCCTGGTTCAATTCCCAACACCCACATGGCAGCTAATAGCCATCTAACTTCAGTCCCCCGGGGGATGCAGTGCCTTCTCACAGTACCCATGTGGTGCTCAGACATGAGTGCAGGCTGAGTATCCACACTCAAAATAAAATAAAAAGAGAGTGGTGGTGACAGGGGTTGAAGGGCTGGTTCAGAGGCTAAAAGCACTGCTCTTAATAAAGGACCCGAATTCAGTCCTCACATCTATTATCAGCAGCTCCCAGGATAACCCATGTTTCCAGTCTCCTCCAGTGACAGATGCCTATACACATATACATGTGGACATAGGTAAAGCTTTTAACTGGCTAAGAGACCAGATGAAACGGATAGTTCTGTGGTCAACAGGACCTGAATTCAGCTCCCAGCATCGATCCTCTGTAACACTCGCTCCAGGAGACCTGACATTCTGTCCTGGCCTCCTTGGGCACCTGGAGACCTGACATTTTCTCCTGGCCTCCTTGGCACCCGGATGCACATAAATGAACATACATTTTTTTTTTTAATTTTTAAAACTTTTTTTGAGATAAGGTCTCTCTACATAGCCCTAAAACTCACTCAGTAGATCAGGCTACCCTCAAACACAGAGATTCTGTCTCTGCCTCCTGAGTGCTAAGATTAAAGGCATATTAAAATCATTTTTTAAGTGGATAAAGTGGTAAATTTTGGGTGTGGGGATGTAGCTCAGTTGAGACTGCTTGCTTATCATGAGACACTGAACTCAACCTTCAGCACCAAATACAATGGCTGTGTGGTACACACCTGAAGGCGGAGAAAGGGGAAGCAGGCAAGCCCTGGCTGCACAAGACATCTCAGTTTGCTGAGACTGTGCTACCTAGGCTGACCTCCAACTTGATACACAGCTAAGGTGAGCCTTGAACTCCTGGTCCTCCTGCCTCTGCCTTCCAAGGGAATTACCAGCATGTACAACTATGCTGAGCAACTTTATACTCTTTAAAGATTTGTTCTTATTATTTCTAATTATACGTATGTGAGCATGTCTGTGTGTAGGGATGTACAGGTGCCCTCACGGGCCAGAAAAGTCAGAGCACCCAAACCCCGAGTCCTAAGCAAGCTACTGACATAGGTGCAGGGGCTTGAACACAGGTTCTCTGGACAAACAATAAGTGCTCTTAAACACTGAGCCATCTCTCCAGCCCCATCTTTTTGTTTGTTTGTTGTTGTTTGTCTTATTTTGAAACAAGGTTTCTCTGTGTAGCCCTAGATGTCCTGGAATTCACTCTGTAGACCAGGCTGGCCTCAAATTCAGAAATCTGCCTGCCTCTGCCTCTCAAGTGTTGGGTTTAAATATATGTACCACCACTGCCCACTTTAAAAAAAATAATAATCCAAATTCCTAATTGCAATTCCCGAACCCCTGAGCCTTCTCCCAGCTGTTCTGCCAACAGGCAGACCTGCTTGACTCTGTCCTCTGCTGAGGCCGTCTCTCCAGCCTCACAACCTTCCCAGTGCTAGCTCCATCATTTCTGGGCAACCCCACAACACATAGCTCACTCATCTGAGCCCCTCCTGACTGCCTATCCAGTGCAGCTGCATGAATCCACTGAAAGTGGACCTGGGCAGAGCAGGGCCAGCTTTGTACTAGAAATAGAACTCAGGACTACGTGCATGTCAGACAAGCTCTCCACTACAGAACTGCACCTTGACCCTCCTGGTCAACATTCATAAAGAGTTTGAGAGTGAGACCTTCCACCAGTGGGAGGTTTCTACAGGCTGCTGAGTCCTGCTAGACTGGCCTCACGTGACACATGCTCCCCCTGTCTTTTGTGCTTGTCACACTCCAAGAGTTGGTCAGTCATGGAGGGGTGTGGCTATGGCACAGACCCAGGTTCTCTTACTTACCTCTCTCCTCATCGAGGTTGCCAGCATGCTGGCTTTCTTGGGTTGAGAGCATACCTAGTGACTAGTGGGTGGTGGCTGGTCCTCATGTGCCCCTGCCTACCAAGTTACTCTCCCCAGGCTGTTGCTCTCAGACAGTGGGAGGCCCAAGAGCATCTTATTTTGTCTGCATATTTTCATTTTCTTTCTTCCCTTTTTAAAATATTATTTCAAGCTTTGGCCTGAAATCAGTTCCTTCAATGGGACCTATATACAAGTGACCTAGGACATCTGTTTGCAACCTCCTATTCCAAGATATAATGTACATGACAGGGGGAAAGACTCACCTAACTACCTCAGGACCCAAATGCAAAATTTCCCAGACCTGACCTCTGACCTCTGGCACATACTCACACATTGCAGCCATTCTTAGAGTCCAAAGAATCCCCAAAGCTTGTACAGTGAAGTTTCCAGGAAGCCTGATAAACCACATTCCCCGGGAGGGAGGACGGCAGGCTAGGGTGCTCTTGCCCAGGTGGGCCTAACAGGACAAACAGAGCAACAAGGCCCAGAGCTGCCCACAAGCACTAGCTAGGGAATGCCAGAGCCACCAAACATGGTGATGAAGCAAGGCTTGTCAAAGCCATTTGGGGTCTTTGAAAAACACCGCCACAGGGAATGGAACTTTTGATTCCCTAAGATGGTGCTAGGCAGGTCTGGCAGGTCAGGGAGCAGTGGGTGGAGTCCTGGGCCCAAATTGGAGGCCCTTAGGGCACCCATTCCAGCATTCACTACTAAATTACCCCAGGCCCCAAGCCAGCATGGTTACATTTGAACTTTGCAGTGGAGAGCTTGGAGCTATGGTCTCCATCTGCCATCTAGCTCAGGGTCAGCCACCCATCACTGTCTCCAGCTTCATCCAGGGACTTCCCCTCAAACTCTCCCACCCACTTCCAACCCTAACACCTTCCTGGGGGTCTACAGGCCTCTAAACAGATCTGACCTACAGAGAAGCTCCTGTACAGCACCCAACTCTTCCGGGTCCCAAGTTAAACCCCATCCCAGCTCTCAGGGCAACAGCAGAAACACCAGCAGCCCGCTGAACACAAGCATAGCTCAAAGCATGACGCAGACAAGGCAAGTCCCACTGTCTGTATTTATCCAAGCCACTGCCTCCCCTCCAGGAGTGATGACTGAGGTTCTGTTTTAGACATAGGGCAGGGCTCTCAGCTTGCTCCCTGGAGACCAGCTCCGTGGACTGCCTGGCCTCTCCAAGTGTCCTTTCCAGCCAAGACCCTCAGTCACGGCCCAGCTTCCTCTGCCTGCCACACCAATTCACCAGAGATGTAAGTGGCCTGGACATGGAGAGTGTCGTCGAGCACCACAAAGTCTGCGGCAGAACCAGAGGATGAGAGGAGACGGTGGAATGGTCCATACCCTACTGCGGGGTCATTCATAACTCAGCCCAGGAAGAAAACTGAGGCCGTCTAGGAATGTAATCTGGACTGGTGGGGGAGCTGGAAGCAAGAGTATCAACTTGGCACAGTGAGGGTCCCTGTAACCCTCACATGGCCTGATGTTATATGCCAGAAAGCCAGGCCAAAAGCAAGTGTCGTAGCACATGCTTGTGATCCTACAAGGCAGGACTGCAGGCTCGTCAGGGCTATATAATAAGACCCCATCTCAAAAACAAAACAAACAAAACAGTAAGGGCCAGTCAGCAGATGACTGAGTCGGTAAAGGAGATGGGGAGGTATGGTTTGCCTCCAAGCCTGACTTAGTTTGAACCCTGGGACTCACATGGTAGGAGAGAACCAACTATAAGTTGTCTGTGCTGGGAGCAGCCCCAGACTCAGCCCCTGCATGGGAACTCACCTGCATCAGCACCAAAGTCCAGGCTCCCCTTGGTCTTCTCCAGCCCCAGCATCTGGGCAGGGTGGAGGGAGGCAGCTTCCAGGGCCGACTCCACACTACAGCCTGCTCAGGAACACACCATGGTGAGCATCCACAACCCTTCCCACGAGCAAGTCCAAGTTTCAGCTTTTAGGGACTAGGCTTGGGAATGGCCCAGGATTATGCAGCAGCTTAGGATGGTCCCTAACGCCCCTTAGCCGGAGGAGACATTGCTCATCCCTCCCTTCTCCAGCCCTGAAGGGAACCTATACTTTCAACAGACTAGACATGGGGCATCAGTTAGGGTGTATTCAGTATTGTATGATCATAGACCCTCTCGCCTACTTCTAATAAAAAAGCCATACCTGGGCCAACCAAGGCCAAATGGGACCCTGTATTCTTAGCAAGGCCTACCCCGAAGCCCAGTGACCACATTCCCCTTCCTGTATCACTTCTTGCCAGGCCATCCCCAGCCTATTTTCTCTAAATGCGACCCTGACGTTGCCCACATTCAGAGTGACACTCAACCTTGAGATGCTGCCAGGTATCTGGGAAGTCCCTGCAGAACCTGCTCAAAGTCCCAGGAAGCCAGTTTCATTCCACCCTGTCCATACTGCCCCTGCAGATTGCCATTATCCCACAGGTCTAGTCCTTGGCCTTTCCATGA
GF3 -1 -1 1 CAGGAACTGATTGACACTGGAGTGGCCTC
GF4 -1 -1 1 GTGAGATCCATAAGTCATATAGAGGAG
EX52 -1 -1 1 GTGGCAGCATAGCCCCCATGGATGTGTGC
EX32 -1 -1 1 GCAGGAAATGCCGGACGCACACATC
GR3 -1 -1 1 CTTGGACCGCGGTTGTTGTGATTGGAC
GR4 -1 -1 1 CTGAGTTCAACTGAACCTCGCAGGC
Comments:
Created
Target: 2803Ensembl ENSMUSG00000036820 -- 24294495 -- 24295661 ENSMUSE00000310103 -- ENSMUSE00000310204
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:17:1:95272651:1, start 24294494: end 24295661
Found 7 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:17:24301161:24302160_151 G3:17:24289995:24290994_798
ACCEPT (0) gap pair G5:17:24301161:24302160_151 G3:17:24289995:24290994_803
ACCEPT (0) gap pair G5:17:24301161:24302160_151 G3:17:24289995:24290994_791
ACCEPT (0) gap pair G5:17:24301161:24302160_145 G3:17:24289995:24290994_798
ACCEPT (0) gap pair G5:17:24301161:24302160_145 G3:17:24289995:24290994_803
ACCEPT (0) gap pair G5:17:24301161:24302160_145 G3:17:24289995:24290994_791
ACCEPT (0) gap pair G5:17:24301161:24302160_164 G3:17:24289995:24290994_798
ACCEPT (0) gap pair G5:17:24301161:24302160_164 G3:17:24289995:24290994_803
ACCEPT (0) gap pair G5:17:24301161:24302160_164 G3:17:24289995:24290994_791
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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