High Throughput Gene Targeting: Create Design

This custom vector design tool is provided by the EUCOMM consortium as a service to the research community. When using this tool, the user agrees not to submit more than five designs per day for creation without prior discussion with the High Throughput Gene Targeting Group at the Sanger Institute.

Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by dk3
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 175324
Chr: 12
Strand: -1
Status: Ordered
BACs: RP24-248E22 : RP24-476B20 : RP24-502D20 : RP24-185C13 : RP24-458M16 : RP24-458I18 :
Instance: Plate 149: Well A03: BACs RP24-185C13 RP24-476B20 RP24-248E22 RP24-502D20
Oligos on GRCm38:
Type Start End Length Seq
G5 84946049 84946098 50 GATTTATCGGTTGTTAATGGACAATATTTGGGGAGATATATTCTTCTGTG
U5 84940848 84940897 50 TTTGCAAAATGCCAGAGAGGAAGTATTTATGGCTTTGTGGACCACACACA
U3 84940712 84940761 50 TATTCTAGCCTCAGTGCTAACCCAATGTGAGGGCAGGTGACCAGCAGCAT
D5 84939951 84940000 50 TGAAATCTGTGACATGTGAGCAAAATCTTAATCTAATTGGTTTTTTGTTT
D3 84939859 84939908 50 GGGTTTCTTGGTACCATAATGAAAATCCTCTAAAATGAACTGTGGTGATA
G3 84936214 84936263 50 ATTAGACATTATACCAAAATCTGTAGAGAAAGGAAAAGGAACTGGAATTA
Oligos:
Type Start End Length Seq
D5_D3 86280859 86280900 42 CCTGCTGCGCAATGAATTTTAAAACCATTATACTTAAATTGA
G5 86286999 86287048 50 GATTTATCGGTTGTTAATGGACAATATTTGGGGAGATATATTCTTCTGTG
U5 86281798 86281847 50 TTTGCAAAATGCCAGAGAGGAAGTATTTATGGCTTTGTGGACCACACACA
U3 86281662 86281711 50 TATTCTAGCCTCAGTGCTAACCCAATGTGAGGGCAGGTGACCAGCAGCAT
D5 86280901 86280950 50 TGAAATCTGTGACATGTGAGCAAAATCTTAATCTAATTGGTTTTTTGTTT
D3 86280809 86280858 50 GGGTTTCTTGGTACCATAATGAAAATCCTCTAAAATGAACTGTGGTGATA
G3 86277164 86277213 50 ATTAGACATTATACCAAAATCTGTAGAGAAAGGAAAAGGAACTGGAATTA
G5_U5 86281798 86287048 5251 TTTGCAAAATGCCAGAGAGGAAGTATTTATGGCTTTGTGGACCACACACAGCTACCCTAACTATGCAGATGAGAAAGCAGCCGAAAGACGAATATGGCTGTGTTCCAACTATAAGGCAGCTACAATGCCAGGCACTCATTGTGGCCCACACCTTTAATCCAAATATCAGAAGCAGGTGGATCTCTATGGTAAGGCTAGCTTAGTCTACATAGTGTGTTACAGGACAGTCAGAGCTACTTAGGGAAATCCTGTCTCAGAAACCACCATGAAACAGAAACTGGGCTGGCGTGGGCTTTCCGGCTCCATTTACTATACTAATCTAGGATGGCTCCTCCCACCTTTCCTTTTACCCTTTTCTTTCTTGCCACCCCCCATCCCTTTTTAAGATAAGGTCACCCAGCATAGCCCTGACCCTGGAACTCATTATGCAGACCAGGCTGGCCAGAACTAACAGAGATCTGCCTGCTTCTGCTTCCTGAGTGCTGGGATTGAAAATTTTGTTTTGTTTTGAGCCAGAGTCCCATATAGTTTAGAATGGGCTTTGAGACCCCCATGTACACAGGATGACCTTGAACTCCTGATCAGAGAGTTCTGCCTCCCAGGTGCAGGCATCACAGCAGGGTGTCCAACCAGCCTCAACACTTTTTTTTATTTGAAAAACATAACATTAGGCTTTCCACTTCCTCAAAGCTATGTACCCAGTAAACCAAAGGATAGGAAAATTCAAGCCTCTCCAGATATTAATAATTCCCAAGTTCCTGGGAGGAACTGAAAACAAGGGAAAATGTTAACATTCCAGCCTGCAGCACAAGACAGCACCGGTCCCTGGAAAGCAAATGACTCAGAAACCATGACGGGTATGGAGACAGAAACATTCCCTTACATGCACTCGGAAGGCCATGCTGTGGCCCACCTCATAGGGGTCCTTCCAATCCCAGGACACTTTGCAGGACCGGGGATCCAGGTAATTTCCCCGCACGTAGTCATAAATAGTCCGGTCCCCTCGGCGCTCACGGTCTTCATTCTGAAGAAAGCTGACTACACGTGCGGCAAGCTCAAAGAGGAACTTAATTGTGAAGAAGAATGCAACCACAGACACTATGATTCCACCTACACGAAAGAGAAACACAAACCACATTACAGCGACTACAGAACAGACTGGCAGCTCACAATGCTTAAAGGAGTCAGAAGTATCTGCAAGATTGTAAGAACGAAGGAGGAGAGGGAGGGAGGAAAGAAGGAAAGAAGGAAGGAAGGAAGGGAGGAAGGGAGGGAGGGAGAAAGGGAGGGAGGAAGGAAGGGAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGGAGGGAGGGAGAAAGGGAGGGAGGAAGGAAGGGAGGAAGGAAGGAAGGAAGGGAAGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGAAAGGGAGGAAGGGAGGAAGGGAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGAAAGGGAGGAAGGGAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGAAAGGGAGGAAGGAAGGAAGGGAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGACGGACGGACGGACGGACACTGTAATGTGTAGAAAGGAGCTTCTAGAGCATAATTAGCTAACTGATCTCAGGACAAACCCCACCCTGGTGTTACACCTCTGTAAATGAAGCTATGCTAGGACATCACCTCACCAGTCCTAAACGAACCACCCAGGCAGAGCTGAGTATGTGGAAGGAGACCGTATGTTTGCAAAACGAGGAATGTATTTGTTCTCTTGCCTTTCACAGACCAGTCTGCTGACCCTGTTGTCTATCACGAAGCCCAGGCACAACCCCTCCCACCGTCCTGCCATGCTGCCCCTCACAGAAGTGCTTAGCATAGTGTGCTCTTGCTTCCCTCCACCTGGAAGCCTTTTGTTTGCTTGTCCACTTGGCAAAAGCCTTCCACAAGTCCCATCAGTTAGGGCTTCCTTCTTTTGGGCCCCCTCAAACTTCTGTATCTACTAACTTCACTTACCTACCTCCCCCTTCGTGTTGTGAACTCCTTACAGGCAGATTTTTGCCTTGACTTGGACTTTACAGTAAACAGTGTCTGGCACACACTATAATTACTCAATAACTATTTCCCAAATGAATGAACTCTGCCAACTGTGTGAGGGTACGTTCTGGCACAAGTGGAGCTATTTCTAGCTCCATGTTCAAGGAGAAGGTGAGATAGGGAGGCAAACAGGAACTCGACAACATTAACCAAAGACAAGGGAAACGATCCAATCACATACCAATAACGTAAAACATCAGGTCTCTTCAACGCCACCGGACAGCCGAGGATCACAGCTGCAACTGCAAAAGAACATCTGTTCAGTGTCTTCCAGGTCTCCTTAAAGCCACCCCAGGAGACAAAGCAGAGCACGTGCCGTAAGCCTACCAGCTCTAGTCTCGTGCCTTAACGTCCCCATCCTCTCTGCCACGCCCGATAGGGGCCCTGTGCCATGTGTCACACAAATAGACTATGGCGCTCTGCCTAAGGGCTTAGGGTCTACTGACCGTTCTCAGAAGGCAGCCACTTGATTTAGTGCTAGCTACATATGAAATGATACAGCTCTCATCTTAGTTCCTCTTCCGTGAAGAGTGCTGCTAATTAGGACAAGGAAAGAGGATAATGGAGCACTTCCCCCATGTTATCTGCCCTCTGAAAGCAATCCAGCTAAGTAGAAAAAACAGCTATATAAATTGAAGATATTTTTTACAAGTAAATATGATCGTCATGCTAGCATCAAGAAAGCAAAGTGAACTCTGGGTCATCCCAATAATAAGTAGCGGGTACAAAGGAGGAGTCATTCGTGACATTACCAGATCAGACAAACGGATGGGCCCTAGGGAAGATGGATTCAACAGAAAGCAGAGTTGTGGAAAAAAAGGAGGGGATGAAACCTTCCATCCTAAGTGGTGTCCACTAAGACCAGAGTCTGAGGGGGAATTTTCACTGTTACTCAACTTGTTTTCCCCCAATTCGTACATCAGCATCCCGCACTCAGAGACTAGACATCTAAAGATTTCTAGACTCCAACCTTGGAGAGCACAGAACAATTTGAAAAGGGAAAGGAGAACAGGCACCTGTGTGCATTTATATCTGATTGTGCGTCAAGGTTTTAAGAGAGAGAGAGGTGACACATGGCTTGAGACACAAGGACAGGAAAGTGCCTACCTGCCTCTGACAATTACAACCTTTTGCCCACCATGAGGCAGCTGGCAAACGGCAAACCAAAACAGGAAGGAAGGGCCAGGGCGATAGCTAATCTACACCTCCATGGGTGTGACAGTTCTTGCTGCTTTAACAAGTTAGGATTTACTATCTTCACCTCCTTAGCATAACTCTGGCATAGTTCATCAGAGTACTGACAGCTCTAAAGGCAGAGTGGAACAAATAGTGAAACACAGAGGAGCAAGCAGACAGATGCCTCCATCCTTCAGTCAGATTCATAAGCTCAAACTGTGACCCTGGCCAAGCCACCTAATCTCTCCACTGAGAGCCAGTTTTCTTCTCTGTCAAATGGAGAGAGTACTCACAGGCACCTCACTTGGGTGTTATTAGGACTTAGTGGGCTAACACGTCAGCCTAGAACTGCAGTGAGCTTTCAGTCTTTGTGCTGCTTATCTATAGAACCAGCATCATACCTCCTTTGCTTCAGAGCTGACAGGAAATTCAGGGAGAAATGTATTAACAATTACAAAGGACATGATTTCTCACTGGATCAACAAATCTTACCCTTCAAGGGAATTCTTTACACTCTTCTAATGTGATGACTGAAAACTTTTATAAGAAGTTAAGAACTCGCATTAAAGCAAACCATCCAACAAATTCTAAGTGCTTGCTGTGTCAGGCACTGGGAAACAGCCCAGAGCATAACATCCACTCTGACTCCAGCAAGAGTCACCTTTGCCTTGTTAAACCCTTGGGCATTGTTTTTCCCTATTAGATTATTTCTCAACCAAATTATTAGTACTGGCTTTTACATTGCCTAGACCTTTTCCAGTAAGTCATTTCTATTGCCTCTACCACAATCCTCTTCACACTTAAAACCCAGGACCAGTGACTACTTTTTCCCAAGGGACTCTCCTCGTCTTAGAACATCTGAGTAAGGCCTGAGCTCTGTCGCTTGCAAACTCACTGTTCTGACCTTTCTAAACCATCCTTTCCTCTAACCTCACAGCCCTAGAGGCTTCCTAAAACACGTACCCTGCTCCAAGTCTCCCTAAGGTCACATCCTGAGCCTGTAATCCTCTCCATCTCCCGCCCTCTCCTTCCTCCTGGCACACAGCCTCGATGACTGTCATGGGTTCCCTCTGCCCTGTCTGTCCTGCTCCACCCTACACTACTCTCCGTAGCCACACCTCACATACTCTTTCATCATTTCTCCTCGGATTCCTGTTACCCAGGTCAGAAACTACACTCGCATATATACTCCCTGAATGCCCTGTCTCCACACATTTTCAAAAAGCCATCTCTTGGTACAGTAAACCTGGTCAAAACCCATGAGTGAGGTCTCAGACCCCAGCAGAGAAGCTACAGCTATAGTTGGGCTAAATGGAAATGATGTCTGTTAAACTCCTTCTAAAGAATGTTTACAGTCACAGGGCACTTTTGTTGTCATTTGTGGTTATTAGAGAACTTTTGATTGACTGTGGACAAGGGTAGCTATACTCATGGCTAGTCAAATGATAAGAATAGTGACTTGAATACATACTCAAGAATAAATGAGACATCTGCATCATCCTATTCAGGGCTCAAGGAGCACTGCAGAAGAGAAGACCACAGAACTTAAGAGGCAAAGATGGAAGAAAGTGGTAGCATACTGTTGGCCAGACGTGGCAGGGCTGCCACACTTTTGAACTCATGGGAGCTATGGCTACCTATACAAGACTTGCATATGATGGAGCCTGTTAACATCCTAGCATGGAGAAGGGATGAACTCACAAGGCCCCAGCCCTTACTGAGGATTTATCGGTTGTTAATGGACAATATTTGGGGAGATATATTCTTCTGTG
U3_D5 86280901 86281711 811 TGAAATCTGTGACATGTGAGCAAAATCTTAATCTAATTGGTTTTTTGTTTTTGTTTTTTTAAAGGAAGAACTGGGAGTCCCATCAAGAGGCAGAGTCCATACTTAGCAGGAGGCCCTAGATCCTACCCTTCCTTACCAGAAAAGGAAAGCAAGCACAAAGAAGGCATGGTAGAGTAAAGGGAGGACCAAGGAATGAACCACGAACCTACCAGGCTGAAAAATTTTGTAGTAGGGACTATAGGCCACATTTAAGCCACCCAACTTCACAGTGATCTCATAGCGCCCAGCCTTGCGCACAGTGAAGGCCACCTTCACCACGTTAGAATTCGGCTCCTGGAGGACTTCCTGGGTCACTGGAATGTCCACTGCTAGTTCAACGTGAGAGATGTGAACCCGCAGTCCCACGGGCCGATGTGCAGGGAAAGGCTGTCCATTCTTATAGAACAACTGCAATGGAAGAGAGGGGTGGGCACCCATTAGTACAGAAGGGGGAGAGAACCACTTCCCATGTGGCCAGGGTGTGGGGCAGAGGAAGCAACCGCCCAAGGTCACAATGGTAAACCCGTGTCCACCTGTTCCTTTCAGTTTTCAGTTCTGGTCTTAGAAACACAGCGAGAGACATGTTTATAAACATGACAAGAAAACCACTAAATCATGGACAAGTCTTTGCCTGTGCAGAGGACGGGGCAGCATACACTACAAAGTCAAGAACACTTGAGGTTTCTGAACAGTAAATGCTGCCTCCTCAGTGTCCCCAGCTCTATTCTAGCCTCAGTGCTAACCCAATGTGAGGGCAGGTGACCAGCAGCAT
D3_G3 86277164 86280858 3695 ATTAGACATTATACCAAAATCTGTAGAGAAAGGAAAAGGAACTGGAATTAGCTAAAGCAATTTTGAAAAAGCAGAGTAAGTGGAGGAGAGTAGTGCCTGTGATCTGACGGGCAGTAAACAAGGCCCTGGTACTGAGGAGGGGGCAGACACAACAACCAACGTCATCATACAGAACCTCAGGCAGACCACCACACCCACCCACCCAGCTGACGAAGGTGCAAAACGTCTATCCATGAAGGAAAGGCAACTCTCTCAACACATGGTCCTTGGGCAACCAGAGAACTTCGAGGAAAAAAAAGATCTATCAAGAACCTTGTCACAAGTGTCACATGCAAAAGTCAACTAAACATGGAGCATGGGTTTAACACCAAAGTGAAACAATAAAACTTTAGAAAAACCAGTGGAGAAAAGTCTTTAGGATCCAGAATTAGCCAGAGACCTTTGATATCAAAAGCATGATCACGAAAGGAAAATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACTTTCTCAATATTAAAAAGGGTTTTACACCATGGAAGATCCTAAGAGGACTAAAAGAAAAGCAGGATTTTAACTGTTGGACAAGGGACTAGAATTTATGCATAGAATTTATGTAAAGAGGCTCTCAAAACACAACAGTTGGAAACCCAAATATTTCTAATTAAAAAATACGCCAACAACATGAAAAGATATTTTACCAAAGATGATAACATAGCTGTCAGATGAGCACATGGAAAGATGTTATCACCCCTTACTATTTGGGAAATGCAAGTTAAACCTACACTCATCAGAATGTTCAAAATAAAAAAGTGACAATATTACAGTGGCAAGGAAGTGAAGAAATAGAGTCCAAATGCTACAGCCACTCTGGAAAAGAGTTTAGCAATTTCTTTCTTAAAAAAAATAAAAATGCAACATCAACTGTCTTGGCAAACATCTTCCAGAGCATTTAAGAAATGCTCTTATAAAAAAACCTATACATGAGTGTTAACAGTAGCTTTGTTGATAATAGCTAGGAACAGGAAACAATCAGGATGACCTTCAATGGATGAATTGTTAAACAAATACCCATACACTGAATGGTACCCACAAAACAGAACCAAGCACTCATACACTTAACAACATAGACAGATCGCCCGAAAATTCAGCCAGTTTTGAGAGGTTACTTCAAACACTCTTCATATGACTTAGTGGAGAACTATGGTTTGGGGCTTTGGTTTTAGACAGGGGCTGTGCATCCCTGGCTGGCTAAGGAAGACCTTGAGCTCCTGGTCCCCCTGCCTCTACCTCCCAAGTGCTGGAATTACAGATCACTGCAGACATGGGTCACTGTATCTGGTTTTTGTAAAAGTAAAGAAATGGAAAATAGAAATGCATGAAATTCAAGAAGAACGAAGACCAAAGTGTGGACACTTTACCCTTTCTTAGAAATGGGAACAAAACACCCATGGAAGGAGTTACAGAGACAAAATTTGGAGCTGTGACGAAAGGATGGACCATCTAGTGATTACCATATGCAGGGATCCATCCCATTATCAGCTTCCAAATGCTGACACCATTGCATACACTAGCAAGATTTTGCTGAAAGGACCCAGATATAGCTCTCTCTTGTGAGACTATGCCAGGGCCTAGCAAACACAGAAGTGGATGATCACAGTCAGCTATTGGATGGGTCACACGGCCCCCAATGGAGGAGCTAGAGAAATTACCCAAGGAGCTAAAGGGAACTGCAACCCTATAGGTGGAACAACAATATGAACTAACCAGTACCTGGGAGCTCTTGTCTTTAGCTGCATATGTATCAAAAGATGGCCTAGTCAGCCATCACTGCAAAGAGAGGCCCATTGGACTTGCAAACTTTATATGCCCCAGTACAGGGGAACGCCAGGGCCAAAAAGGGGGAGTGGGTGGGTAGGGGATTTGGGGGGGTGGGTATGGGGGACCTTTGGGATAGCATTGAAAATGTAAACGAGAAAAATACCTAAAAAAAAAAAAAAAAAAAAAAGAAATGGAAAATAGATCCCTGAGTTTCCAGGACGTCAGAAACAAGCCTGGTGGGGGTGGGAGAAAGATGAATGTAACTACAAAAGGGCAACACTGGAAAGATCTGTACAATGCCAGAAATATTTGTGTCCTTTAAAAAGCTTTTTAAGTTTTACTTGTATTTTATTCATATGTGTGCTATGCCTGCATGTATGTGTGTGCACTATGTACACACCTTGTGCTCATGGAGGCTAGAGTTACAAGATGGTTGTGAGCCATAGTCTGGGTGCCAGGAACTGAACCTGGGTCCTCAGCCAGTGCTCTGAACTGCTGAGTCATTTCTCCAGCCCCATGTACCTTAACTGTATTAATGTCAGAAACATGGCTGTGGTGCTCTGCCATATCACCATCACGCTACAATCTGAAAGATGTGACCACTGGAAAGCTAGATAAGGGTATCCCCTATCTCTTATCTATCATGCATGAGAATATAATTCTCAACATAAGTTTAATTTAAACAGAGATGGTACATAATGATACAGATTGTTCTCCATTGGTGGCAAGAAGTATGTGGCAAATGAAGTGGACAGTCTCTGAATGAGGCATTCAGGCAAACGAGACTGATAATGCAGACAGCTTGGCCAGTTTCCTTTGCTCTAGAAAAAGAGAACTGGGCACCAGCCAAACAGAGACTCCAATCATTAGGATTTAACTCCCAAAGTTAGCCAATGTCCTATTATTTCTGTGGATACCAAACTAGCCAGTATCTAATGGCATCATCAAAAATGCTAGAAAGGTACAGGCAGATAGGAGTTCCAAACACTAAACACTAATATTGCAGAACCAAGAGTCAAGAGTCAGAAATTACAGTTTAGACTTCAGTGCCAGTGAGCATTTGTTATCTGGCACAAAGGAAGGAATCACACATGGACAGATGCTCTTCGGGATGAAGTAAAATACATATTGACAGTACTAGTGGGTTTGGGCCAAGGTGCTTTTACTTAAAAAGGTCTTTAAAAAAAATCAGAAGAATGTATCTCCTTTCTATTCTTGTATCCTAGGCCCTCCAGAGCTATCAGACTGACTACTCAGCAATTCTATTCCTGGAGAAATCCAAACACATTGCCCACACAGAGACTCAAACACGGAGGCCGGAAAGACCACAAAAGCGATACTACTTCTCTTCTTTGGAAAGAACACATTGCCTCCAAGCCTGCTGGCCTGCGTGTGATCCCCAGAACCCACACGATGAAGGATAGAGCTGACTTTACATGCTGTCCCCCGACCTCCACATGAGAACTGTGGCCTGGATCTGCCCCTCCCCCAATAAATAAGTCAATAAATGGATCCTGGGTGGGTAGATAGATAGAGAACCTGTACACAAATGTTTACAGTAGTCAAGAAGTCACATGGAAAGAAACAACCTAGATGTCCACCGCTTACAAAAAGACAAACAATACACGGCATGTTACTTGGCAAGGAAAAGGGATGAAGGCCTAAACATGCTACAGCACAAGGGACCTGAACAGTGCTCGTGAGGTCAAACAACAGTCAAAAGGCCTCGTAGTTTATGATTACACTGATAGAACTGGCCAGAGAACTCAACTTCACAGCATCTGTAGCTAGGGGTTTGGCAACAAAGTTGCTAATGGGCCTGGGGTTTCTTGGTACCATAATGAAAATCCTCTAAAATGAACTGTGGTGATA
U5_15 86281798 86296848 15051 TTTGCAAAATGCCAGAGAGGAAGTATTTATGGCTTTGTGGACCACACACAGCTACCCTAACTATGCAGATGAGAAAGCAGCCGAAAGACGAATATGGCTGTGTTCCAACTATAAGGCAGCTACAATGCCAGGCACTCATTGTGGCCCACACCTTTAATCCAAATATCAGAAGCAGGTGGATCTCTATGGTAAGGCTAGCTTAGTCTACATAGTGTGTTACAGGACAGTCAGAGCTACTTAGGGAAATCCTGTCTCAGAAACCACCATGAAACAGAAACTGGGCTGGCGTGGGCTTTCCGGCTCCATTTACTATACTAATCTAGGATGGCTCCTCCCACCTTTCCTTTTACCCTTTTCTTTCTTGCCACCCCCCATCCCTTTTTAAGATAAGGTCACCCAGCATAGCCCTGACCCTGGAACTCATTATGCAGACCAGGCTGGCCAGAACTAACAGAGATCTGCCTGCTTCTGCTTCCTGAGTGCTGGGATTGAAAATTTTGTTTTGTTTTGAGCCAGAGTCCCATATAGTTTAGAATGGGCTTTGAGACCCCCATGTACACAGGATGACCTTGAACTCCTGATCAGAGAGTTCTGCCTCCCAGGTGCAGGCATCACAGCAGGGTGTCCAACCAGCCTCAACACTTTTTTTTATTTGAAAAACATAACATTAGGCTTTCCACTTCCTCAAAGCTATGTACCCAGTAAACCAAAGGATAGGAAAATTCAAGCCTCTCCAGATATTAATAATTCCCAAGTTCCTGGGAGGAACTGAAAACAAGGGAAAATGTTAACATTCCAGCCTGCAGCACAAGACAGCACCGGTCCCTGGAAAGCAAATGACTCAGAAACCATGACGGGTATGGAGACAGAAACATTCCCTTACATGCACTCGGAAGGCCATGCTGTGGCCCACCTCATAGGGGTCCTTCCAATCCCAGGACACTTTGCAGGACCGGGGATCCAGGTAATTTCCCCGCACGTAGTCATAAATAGTCCGGTCCCCTCGGCGCTCACGGTCTTCATTCTGAAGAAAGCTGACTACACGTGCGGCAAGCTCAAAGAGGAACTTAATTGTGAAGAAGAATGCAACCACAGACACTATGATTCCACCTACACGAAAGAGAAACACAAACCACATTACAGCGACTACAGAACAGACTGGCAGCTCACAATGCTTAAAGGAGTCAGAAGTATCTGCAAGATTGTAAGAACGAAGGAGGAGAGGGAGGGAGGAAAGAAGGAAAGAAGGAAGGAAGGAAGGGAGGAAGGGAGGGAGGGAGAAAGGGAGGGAGGAAGGAAGGGAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGGAGGGAGGGAGAAAGGGAGGGAGGAAGGAAGGGAGGAAGGAAGGAAGGAAGGGAAGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGAAAGGGAGGAAGGGAGGAAGGGAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGAAAGGGAGGAAGGGAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGAAGGGAGGGAGAAAGGGAGGAAGGAAGGAAGGGAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGACGGACGGACGGACGGACACTGTAATGTGTAGAAAGGAGCTTCTAGAGCATAATTAGCTAACTGATCTCAGGACAAACCCCACCCTGGTGTTACACCTCTGTAAATGAAGCTATGCTAGGACATCACCTCACCAGTCCTAAACGAACCACCCAGGCAGAGCTGAGTATGTGGAAGGAGACCGTATGTTTGCAAAACGAGGAATGTATTTGTTCTCTTGCCTTTCACAGACCAGTCTGCTGACCCTGTTGTCTATCACGAAGCCCAGGCACAACCCCTCCCACCGTCCTGCCATGCTGCCCCTCACAGAAGTGCTTAGCATAGTGTGCTCTTGCTTCCCTCCACCTGGAAGCCTTTTGTTTGCTTGTCCACTTGGCAAAAGCCTTCCACAAGTCCCATCAGTTAGGGCTTCCTTCTTTTGGGCCCCCTCAAACTTCTGTATCTACTAACTTCACTTACCTACCTCCCCCTTCGTGTTGTGAACTCCTTACAGGCAGATTTTTGCCTTGACTTGGACTTTACAGTAAACAGTGTCTGGCACACACTATAATTACTCAATAACTATTTCCCAAATGAATGAACTCTGCCAACTGTGTGAGGGTACGTTCTGGCACAAGTGGAGCTATTTCTAGCTCCATGTTCAAGGAGAAGGTGAGATAGGGAGGCAAACAGGAACTCGACAACATTAACCAAAGACAAGGGAAACGATCCAATCACATACCAATAACGTAAAACATCAGGTCTCTTCAACGCCACCGGACAGCCGAGGATCACAGCTGCAACTGCAAAAGAACATCTGTTCAGTGTCTTCCAGGTCTCCTTAAAGCCACCCCAGGAGACAAAGCAGAGCACGTGCCGTAAGCCTACCAGCTCTAGTCTCGTGCCTTAACGTCCCCATCCTCTCTGCCACGCCCGATAGGGGCCCTGTGCCATGTGTCACACAAATAGACTATGGCGCTCTGCCTAAGGGCTTAGGGTCTACTGACCGTTCTCAGAAGGCAGCCACTTGATTTAGTGCTAGCTACATATGAAATGATACAGCTCTCATCTTAGTTCCTCTTCCGTGAAGAGTGCTGCTAATTAGGACAAGGAAAGAGGATAATGGAGCACTTCCCCCATGTTATCTGCCCTCTGAAAGCAATCCAGCTAAGTAGAAAAAACAGCTATATAAATTGAAGATATTTTTTACAAGTAAATATGATCGTCATGCTAGCATCAAGAAAGCAAAGTGAACTCTGGGTCATCCCAATAATAAGTAGCGGGTACAAAGGAGGAGTCATTCGTGACATTACCAGATCAGACAAACGGATGGGCCCTAGGGAAGATGGATTCAACAGAAAGCAGAGTTGTGGAAAAAAAGGAGGGGATGAAACCTTCCATCCTAAGTGGTGTCCACTAAGACCAGAGTCTGAGGGGGAATTTTCACTGTTACTCAACTTGTTTTCCCCCAATTCGTACATCAGCATCCCGCACTCAGAGACTAGACATCTAAAGATTTCTAGACTCCAACCTTGGAGAGCACAGAACAATTTGAAAAGGGAAAGGAGAACAGGCACCTGTGTGCATTTATATCTGATTGTGCGTCAAGGTTTTAAGAGAGAGAGAGGTGACACATGGCTTGAGACACAAGGACAGGAAAGTGCCTACCTGCCTCTGACAATTACAACCTTTTGCCCACCATGAGGCAGCTGGCAAACGGCAAACCAAAACAGGAAGGAAGGGCCAGGGCGATAGCTAATCTACACCTCCATGGGTGTGACAGTTCTTGCTGCTTTAACAAGTTAGGATTTACTATCTTCACCTCCTTAGCATAACTCTGGCATAGTTCATCAGAGTACTGACAGCTCTAAAGGCAGAGTGGAACAAATAGTGAAACACAGAGGAGCAAGCAGACAGATGCCTCCATCCTTCAGTCAGATTCATAAGCTCAAACTGTGACCCTGGCCAAGCCACCTAATCTCTCCACTGAGAGCCAGTTTTCTTCTCTGTCAAATGGAGAGAGTACTCACAGGCACCTCACTTGGGTGTTATTAGGACTTAGTGGGCTAACACGTCAGCCTAGAACTGCAGTGAGCTTTCAGTCTTTGTGCTGCTTATCTATAGAACCAGCATCATACCTCCTTTGCTTCAGAGCTGACAGGAAATTCAGGGAGAAATGTATTAACAATTACAAAGGACATGATTTCTCACTGGATCAACAAATCTTACCCTTCAAGGGAATTCTTTACACTCTTCTAATGTGATGACTGAAAACTTTTATAAGAAGTTAAGAACTCGCATTAAAGCAAACCATCCAACAAATTCTAAGTGCTTGCTGTGTCAGGCACTGGGAAACAGCCCAGAGCATAACATCCACTCTGACTCCAGCAAGAGTCACCTTTGCCTTGTTAAACCCTTGGGCATTGTTTTTCCCTATTAGATTATTTCTCAACCAAATTATTAGTACTGGCTTTTACATTGCCTAGACCTTTTCCAGTAAGTCATTTCTATTGCCTCTACCACAATCCTCTTCACACTTAAAACCCAGGACCAGTGACTACTTTTTCCCAAGGGACTCTCCTCGTCTTAGAACATCTGAGTAAGGCCTGAGCTCTGTCGCTTGCAAACTCACTGTTCTGACCTTTCTAAACCATCCTTTCCTCTAACCTCACAGCCCTAGAGGCTTCCTAAAACACGTACCCTGCTCCAAGTCTCCCTAAGGTCACATCCTGAGCCTGTAATCCTCTCCATCTCCCGCCCTCTCCTTCCTCCTGGCACACAGCCTCGATGACTGTCATGGGTTCCCTCTGCCCTGTCTGTCCTGCTCCACCCTACACTACTCTCCGTAGCCACACCTCACATACTCTTTCATCATTTCTCCTCGGATTCCTGTTACCCAGGTCAGAAACTACACTCGCATATATACTCCCTGAATGCCCTGTCTCCACACATTTTCAAAAAGCCATCTCTTGGTACAGTAAACCTGGTCAAAACCCATGAGTGAGGTCTCAGACCCCAGCAGAGAAGCTACAGCTATAGTTGGGCTAAATGGAAATGATGTCTGTTAAACTCCTTCTAAAGAATGTTTACAGTCACAGGGCACTTTTGTTGTCATTTGTGGTTATTAGAGAACTTTTGATTGACTGTGGACAAGGGTAGCTATACTCATGGCTAGTCAAATGATAAGAATAGTGACTTGAATACATACTCAAGAATAAATGAGACATCTGCATCATCCTATTCAGGGCTCAAGGAGCACTGCAGAAGAGAAGACCACAGAACTTAAGAGGCAAAGATGGAAGAAAGTGGTAGCATACTGTTGGCCAGACGTGGCAGGGCTGCCACACTTTTGAACTCATGGGAGCTATGGCTACCTATACAAGACTTGCATATGATGGAGCCTGTTAACATCCTAGCATGGAGAAGGGATGAACTCACAAGGCCCCAGCCCTTACTGAGGATTTATCGGTTGTTAATGGACAATATTTGGGGAGATATATTCTTCTGTGACGTAGACACTGGTAAAGTGCCCATACTCCTGCAAATATAACCAAAATATATTATATAGATATATTTAATTAATAATGTCAATAAAAACTTTGTAAAAAGTCATGTTCACTGTTTACTCAGCCTACTATTTTAGTTTCCGTTCATGGAATAACTCCTGACCTCAAGCTGTAGGAAAAAGCCTAACTCCTTACTCCTTAAAATTGATAATTTTCAATTTAGAAATTCTTTCTACCTCTAGATTTATATATATACAACTTTTAAAAATTTATGGCATTTACATTGTTTTATTCTTGTTTTGTTTTTCATTTCAAAAAGGTAACTGATATGAGACAAAAACTAATCTTAAACCTTCTCTTATGGGAGCTGAACACAGCTTAGTTGGTGCCTCACACACAGGCACAGGGACCTGAGCTTGGGTCCCCAGAACCCATGTAACAAAGCCTGCAACCCCAGCCCTGTGGGGGCAAAACCAAGCAGCTCTGATGCTCACTGGCCAGTCAGCCTAGCCACACTGATAATAAGCTTCAGGGTTAGTGAGAGATTCTATCTCAAAAAAATAGAGGTGAAAAGCCATCAAACAAAGACACTTATCAACCTTGGGCCTTCCCACCTGTGTGTGCGTGTCTTAGGGTTTTCAATGCTGTGAAGAGACACTATGACCAAGGCAACTCTTATAAGGGACACATTTAATTTGGACTGGCTTACAGGTTTTGAAGTTCAGTCCATTATCATCATGGCGGGAAGCATGGCAGCAACCAGGAAGGCATGGTGCTCAAGAAGGAGCTGAGAGTTCTACATCATCCAAAGGAAGCCAGGAGCAGACTGGCTCCCAAGTAGCTAGGAGGAGGGTCTCAAAGCCCACCCCCACAGTGACACACTTCCTCCAATAAGGCCACACCTACTCCAACAAGGCCACACCTATTAGTGCCACTCCCTGGGCCAAGCATATTCAAACCACCACAATGCACATTCTATACACATACACAACACATACAATAAATATTTTATATCCTCATAAGCATCCAGTCTAATTATAGATAGAAACTATAATAAATATGAATAAAGAATTGGTTAAATAGATATTGTCAACTGTCTGTTCTTTTCTTACCTCAGATGCCTAAAGTTCAAGAGTTTTGCAGAGGGAACAACCTGTCTTTTAGATACAGCTTAGTTTTGATTGTTGTGGTCCTGGAATGGACACCAAGGCCTTGTACATCATCAAAAGTCTACCACTAAGCTACACTCCTAACCCTAATACAATGTTTGTTTGTTTTGAGATAGGGTCTCTCTACATAGTCCTACCTGTACTGGAAACCACTATGCCAAACAGGCTAACCTCAAACTTACAGAGTTCCACCTGCCTCTGCCACCTGAGTACTGGGATTAAAAGCGTGTGGCCACCACATCCAAAGCCTAATACAGTTTCTAAAACATTTAATTACCAGTATATTTGCTTGAACCAATGTATTCATTAAGCTCTTTAAGGCCCAAAATTCTTAATTTCCTTCAGTTCAATAAGTATATCAAATGTCCACAAGGAGTTTGTATCTACAGTACCATCTACTTTCTGTTGGCCCCACTTTCATACAAACTACACTGGAACCTTTCCACTCTAATCTATGCTTAAGAAACATGGTGAACATACTACTCAACACAACTTACTTCCAAGTTAGCTGACATTCCTCTCTACAGTATTCTGGCCCTTATAATGTCTCACTCTAAGCCTGAAGACTAACAGTAGTACTGATAAACTAGTAACTCATGGGTATGTACACCTGCAGCCAGTGAGCCACATATAACTCAAGAGAACTCTGAACTTAGCCTAACACAAAACCACTAACTTACTTAAGCATTATGAGATTTTCTTTTTCTTTGTTGGAAACAATGGACAGTTCTAGAGTATGACCTCTGTAGATGACCAGGTTGCATCACAATAGTCAAAGGTTGGACTTGTCTCAGTGAATGAGGGCTGCTTTAGCACTAAGAACTAAGCCAACCATTGCCCAATGGGCTTTACACATCTTACCCTACGAACCTAACTGTGACCAAAAGATAAGCACTATTATTCTCACAGGTCAAGCAAGCAAAACAAACAAGCTGGGCCTAGAAAAGTTAAGCTGATGAGGCTCTCCTGTAAAAGTGATTCTTGGGGATTTTCTCTGCCCCTTTTGTACCTGAATAAGCAGGGCATTTCTATGCCTGCTCTAGGACATTTTAGCTCCCAAATAAAGACATAAAGACCTGGTATTTTCATTCTGATCTATTCTAACCCAACTAAGCTGCTACTAACCAGCCAAACGTCCCATTACTTGCCATCTGGTCTTGCCCTGGTTGGCTCTGGTCCACCTGTCCTCTGCAATCTCATGGCAAATCCTCCTGCTTTCTCCATGTGGCTTCTCCCCTCCTCCTGTTCCTCCTCCTCCTTCCCCTTTTCTTCCTCCTCCCTCTCTCTCCCTTCCTTTGGGAGTCTACACACACACTGGGGTTGGTAATCTTGCACTGTTCTCTACTTTGCTCAGCTATTGGCTGAATCAGTTCTTTATTGAACCAATCAGGGAGTAAGAGAGGGCATGTTTACAAGATACTGAGGCAGGAGATGCTTCACGAAAATGACCAAAGTCCAGATTGTTCTCAATTCTGAGCACAGAAATCAGCATTTGAGTAATACAAGGACAATCTTTACACAGTGCCTAAAAACATCACTCCTATAGTGGTTTAAGTAACAATCCCATGTCTAACTGAAGCCTTCAACTTTGTACCCGTAATTCTGAACCATGAAAAAGAATGAAGTAATAGAAATAGCCAAGTATTTAGAGAAAAGGGATTTAAGAAAACAATTAAGACAGAAAGAGAGAAGTCATCCAACGTAATTATGAGGAATCTCACAGCAAAGCTAAAATCAGGACTACAAATTCCTCCAGCTTTTTTGTTATCTTCTGAAGTAAAAATAACGCATGGCAAGCCAAAGCAGAGGCGGTGCCAGCTATCTAGTACAGAGAAAGCACTCGATGATGCAGGGTCACAGGCAAGCTGCTTAATGCTAGAGCTTACTTTAGCTCACAGGACTTATAAAAATGGAGGGAATTCTTGAAAATACCTATCTTCAGGAAATGCCAAAATGATAACAGACATACACATTCTAAACTCTGCAACATCCAATACATTTTAGCCCCACTGCTGAAGATCAGTTGTAAAAAAGTCATTTTTCAAACCTGAAGAATTAGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGGGATCCAATGCACTCTTCTGAAGTCAGCTGCAGCATACTCACATAAATTAAATAAATAATTAATAATAAATGAAGAATTAGTTCAAAACTGTTCTGCTAGAGCCAGCTGAATCTCACTGCAGCCTGTGTTCCCCCCGTTCTCTCTTGCCAGAGGCTGAGGGTCTGATCACTGACCCACACCCTCCACCACAGTAGCTGAGTCTCAGCACAGTCTATTAGCTCGGTGTGGAGATCAGGTTGCGCCCTGTCCCAAGTTACTCTGTTGTATAACAAAGTGACCCAACCTAAGAAACTCTGATTTTATCAGCAGCAACTGACTCCAACTTGAGGACGGAGGATCAAGGATGACTACCCCTTGGTGGACAGGTAATCTACCTAGACCACCCGTGAGCATACCCTGACACATAACTATCTTCCTCAAAGTAGAAAGGCCCCTCTATATTGACTGGAGTGAGTTAGACCACAGGAGCACAGGTGTGGATGTGAATCTTTTTAGGAAACACACCAGACTGAGGAAAGGGTGCCCCTCTCTCACTTGAACCACCCAGGGTGTGTGCATGTGCGTGCACGCACGCATGTGTGTTGCACAGTAGTTTCAGCTCAGCTCACTCCCTACTGCTGACATGGCTTTCTCAAACTGCTGCCTGTCTCGCCATTTCTTCATCTCTCTCTGTCCACTCATGTGGATCTTGTCCCACTCTTCGCCTCACTGGTCTGCACCTTACTTCTGTGGCTTTACTAGACCCCAAGGCTAATCCAGCTCTGATCCTGCAGCCTAACTTTGCAGTAGCAGTAGCCCCAGTGTCCAGCATGTTCACATCTTTTTTTTTTAGATTTATTTATTTATTATATGTAAGTATACTGTAGCTATCTTCAGACACTCCAGAAGAGGGCATCAGATCTTATTACAGATGGTTGTGAACCACCATGTGGCTGCTGGGATTTGAACTCAGGACCTTTGGAAGAGCAGTTGGTGCTCTTAACCGCTGAGCCATCTCTCCAGCCCCTGAACATTGATTTTTTTTTTTAAGATTTATTTATTTTATACAAACGAGTACACCACCATTGCTCTCTTCAGACACACCAGAAGAGGGCATCAGACCCCATTACAGATGGTTGTGAGCTACCATGTGGTTGCTGAGAATTGAACTCAGAACCTCTGGAAGAGCAATCAGTGCTCTTAATGGCTGAGCCGTCTCTCTAGCCCCCGCATGTTCACATCTTATTCCTACCAGCTGCACAGGCCTCTCTGAAACACTGAATCTATTCAAACCAATTTACCCCTCTATAGCCTTCAACTGGAAGAAAGTGTGTGTGCATGTATATATATATTCAATACATATTATATATACAATAGATAATATAGCTATATTTATATCTATATAGATATACAGAGAGAGTGTTTACTCCATGATGTATAATGTGTACGCTGAGGGTAAATATTAGTAATTAACATTAGGGTAAGAGAAGTTGTGTTTGTCTTGGCGAGTTAGGAAAGTGGAAGGACTTGGCAGACTGCCACCCTCAAAGCCAGTGTACAATGTGAGTTTACTGTTCTCCAGCCGATAGACATTGTGGAAAGGTGTGTGCTATGTTTCCGACAGTGCACTTGACGGTACTTACTTACCTTTAAGCGAGGGCCCATGTTCTAAGAACCAAGTTATTCACCAAAGCAGCAACTTTCCACTCCTTTCACTTGTCTTCTAACTTGCACATTTGAAAAAAAAAAAAAGAAAAGAAAAAGAAAAGGCCAAAAAGTCACCAGAAAGGATCTGCCCTCCAAATCTTCATTGAGGTTAAGTGTGTCCCAGGAAGCAAAGAGTCAAGTGGTTTTGGACTGAAGCATTTGAATGCTGGGTGAAGAAATAGAGCCCAAGAAAGTATCATTCCTGGGCTCCTCTAGCGAAAGATGTAACCAATGCTGAAAGAGGCATCTGTTCTGGAACCAGGATCCTCCTAGAAAGAAAAAATGGTAAGTTCAGTAAGGTTTTGCTTCTGCAAGAGATTTTACACACACACACACCACCACCACCACCACCACCAAAAAACTACCCTAGCAACAGAGGAGCTACCCATGCCATAAGTACACTAAAGTAATCAAAAGTTGGTAAATTCTGACCAAGCTTTCCAACACAAACGTCATAGTTCTGCAGCTACTTCACCAAGTCCACCAAGGTCCCCTGGCAATCTCTCCACAGAACCTCACGCTCCTCAAACGCCCCAGGCTTTCAGCAGACGTAAGCTAGGGTACAGATTTAAAGAGACTCATCACAGCCTCATGCCTGACTGCAGCTCAATGGGGCTGGGACACAGGGCGTGGGAAGCATGGGGAGAGAACTGAGACGGAAGCAGGGCAGAGGCAGCTTTCAGAGTGCACACCATGGAACGACTCAGTCCTCAGCTGGAAAGAAGTGCCAGCAGAGCTTTAAAGACTTTATGTCCGCCTAGGCATGCCACTTCCTCTGCACTCAAGCTCACAGTTCTAAGACATGGTAAAAGATAACAATATCACTAAAATTAAAACTCTGGGCTGGCATGGTAGTATGAGCCTGTGATCCCAGCAGCACCTGGGAGGCTAACGCAGGATGAGCACGAGTCCCAAGACCAAAAAGGGGAGCTACAAGTTGTCAACTTTAAAAATAAAGTTACCAAGTAAAGTCGCTTCATAACACTCACACATTCAGGGACCAAATGTAGCAAAAAGGGGGTTGTGGGTGCACATATACAAAGAGCTACCATAAAGGAAATGAAAAGGGGCTACTAACCAGGAAATGGGAAGAGACAGCCTCGCCAACAGCACAGACTATTACCAGCCTTCAGGATTTCCAGTGCATATACAGTTCTCTTTCTTAGGCGCAGATACAGGACTACTTAATCAGACTTTACAGTCCAGACGTAACGAGTAAGATACTTTCCCAGTTAACTCCCAAAAGCAGCAGACAGTCTGGTCCCAGAGTCCACATTTTTAATGACCATTCTCCTATCTTTCTGCTAAGAGCAACCAAGGGAGAAAACACAAAATGCTAAACAAACCAGAACAGAAGGACTGGAGGCCAGCAGGTCAAATAAGTTGGCAGACTTTTATAATTAAAAATGGGTAAGTATATGCAAGTATTTTAACACATGGTGATGGACTTGCCAAGTACATGAGCCAAAGCATGGGTCACCCAGCAACTGGAACCAGTTTAAAGGATCTGCAAACACGGGAATAGTTATGTAAACTACTAAAGATCTTCATGATAGAATGTTATATGATAATTTAAAAATGAAACTTTTAAGGAACAAAAACACCAGATTGGGAAATGTTCACAAACTATTGAATGAAAAAGCAAGCTCATCATTGTTCATATAGTACAAGCCCAATCTTAGAAACAGCAGCATTATGTAGTTATATAAAGACTTTAGAAGGATGGTGGGAGACATTCCTCAGTGATTAGCAACAACTGCTCCTCTTGCAGAGGACCCAATTTCCATTCCCTGCACCCACACAGGACAGTTCACAACCACCTACAACTCCAAATCCAGGGGATCTGGTGTCTTTTGGCACCCATGGGTACACATACAAATGGCATATATATACATACACATTAATAAAAATATTAAATAAATCTTTTAAAATTATTTTTTTAAAAAATCAGAAGAGGAGGGCTGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCTGAGGCCCTGAGTTCAATTCCCAGCAATCACCTGATGGCTCACAACCATCTGTAATGAAATCTGATGCCCCCTTCTGGTGTGCAGGTGTGCATACAGATAGAGCTCTCATACATAAAATAATAATTTTTTTTTAAAAATCAGAGGAAAATAAAAATGTTAATGGTTGACATCTCTTGGAATACATGTAATTACAAATAATTTTCACATTCTACCTATATTATTTTGTTTTGTTTGAGATATAGAGTCTCATGTAGCTCAGCTGGCCTCAAAATCACTATATAGCCAACCAAGGCCTTGACCTGATGTGTACCACTATGCATGGTTTATCTCTCTCTTTTTTTAAAGATTTATTTATTTATTCTATATATATGAGTACACTGTAGCTGTACAGATGGTTGTGAGCCATCATGTGGTTGCTGGGAATTAAACTCAGAACCTCTGCTCGCTCTGGCCCTGCTTGCTCCAGCCCAAAGATTTATTTATTTACTATTATACATAAGTACACTGTAGCTATCTTCAGACACGACCCAGAAGAGGGCATCAGATCTCATTATGGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAAATCAGGACCTTTGGAAGAGCAGCCGGTGCTCTTAAACTGCTGAGCCATCTCTCCAGCCCCCAAATATGTAGCCCAGGCTGGCCTTGAACTCATGATCCTCCTGCCTCTGCCTCCTTCAGCAAATCCTACTGGCATATGCCACCACAACCGGAGGTTTATCTCTTTTTAAATATTTGAGAGAGAGAGAGAGAGAGAAAGAGAGAGAGAGAAAGAGAGAGAATGGATAAGTGTGCATGGGTGCAGGTGCCTGTAGAGGTCCTGTGGGGCTGGAGCTCTAGGCAGTTGTGAGCTGCCTGACGTTGGTGCTGGAGACTGACCTCGTCCTCGGGGAAAGCAGTACACATTCTTAATCACGGAGCCATCTCTCCAGCCCCAGTTTTCCAAATGCTTAAAAGACAAAATAAAAAGTTGCTATTTCTGACGAATATTAATTTTGGGAGACCTACTTTCTTTTAAAGAGGGGTAGAGAATAGGAAAATTGCCTTTTGGGGTTTTCTGTTTTCTCCTTTGGAGCTAAGGTCTCATGTGATCAACCTGGAAACGCAAGGGTGACTTTGCACTTCCCCTCCTGTCCCTACCTCCCAGTACTGGGATTATAAACTAAACAAACACCATCTTGCTGGGTTTAAGTTATCTATTTTCAAATAATGAAATGACATAAAGAAGTTAAGATAAAGTCCGGTGGTGGTGGTGGCGCACACTTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATCTCTGAGTTCAAGGTCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACCTTGTCTCAAAAAACAAAAAAACAAAAAACAACAACAACAAAAAGATAAACCATTACTGAAGTTTTATTTCTATTCATTATGATTTTGGTGGCGCTCCAGTTCTGTGTTCAGCTACTTGGTTATTATAACTAGTTCTAATATACTCTGTCCCTGCTATGAGCATGCTTATCCCCATGTTTCAGTAACTCTAGTGAGCTTCAAGACTCAACCTAAGCATTGCTACCCGTTTCCAGCCCAGCTTCTCTATGGGCTTAACTTAAATGTTTCAGAAGCACTGCCTCAACTGTAGAGCAGGAAGCCCAGTGCTCCCCGTGTTCTAACAGCCTTACAGGGAACCCTTAGTGCTGCTGGCATCAGAGACTATACTTTCAGGTACAAGAGACTTAAAGCAAGAGTTGGTAAAATAATTTGTGGGTCAAATTTATCTGTTTGGTCTTGTTCTTTTTAAACACAAACAGAATAAAAATGTCCAAGTTCATTCGTTCATATACCCAATCTATACTCAGTTTTTGCATAAGGCAGAGCCAAGTAGTTGCAGTAGAGGTGTATGTATATATTATCTGAGGTGTTTGAGGCAAACAGTACATATACCTGGTTTTGAGACACGGCCTCTCTACATAGTCAAAAATTATTTTCTTCCCATTCTACTATACCTCTTCTAAAAACAAAAGCTAGAAAACTCAAAAAATTATGCAAAGGCTTCTCATTTCAATTCTTTTCCCTCTCCTACGGTTGGAGAACAGGCTTTCAATATGCAATACAGGCTAGCCTGAAACTCACCACCCTCCTGTCTCTGCTTCCCAATTGCTGGGCTTACACGTGTGTATAACCGTCCCTGGCTCTTATTTTAGCTCTTATGTAAAAGCTTCATGTCTGTATTCTTAGAACAACAAAGAATTTTATTTAATCATTTCCAAAGGCTAAGCAAGAGGATTTTTGTTATACAATATATCTGAAGAAGCAAATAAATTATTGTTCCAAAAATTATTTTAATAACATACTAATGACTTGGTCTGTTTTCAAAATGCTATTCATATATTTTAAAGATTTATTTAATTTATGTTTATGAGGGTTTGCCTGCAGGTATGTATGTATGTATGTGTGCACCTCATGTGTGCTTGGGGGAGATCAGAAGAGGGCATTGGATCCTCTGGGACTGGAATTATGAATGGTTGTAAACCACCGGCTGGGTACTGGGAATCAAACCCAGGTCCTCCACAAGAGCAGCACATGATCTTAACCACCAAGCCATCTTTCTAGTCTTTAAAATGCTATTCTAAGAAAACAAGAAAACACCCATGTGAAAGTTTACTTCCAGTGCATTTAGAGGTGGTAACAGCATGCAAGAATTCTGTTGTCACCTACTGGTTTCCACAAAACAAGATGGAA
D3_15 86265810 86280858 15049 AAAAAAAGTTCTACTGTTAGTCTGTTGAGAATCTTGCAGGTGAATAACCTGCTGCTGTCCCTACACCAGGAACTGTGAACAAAGTTCTGTTCACTAGCTATTTTGTTACTCAGAGCTCACTAGTACAAAGACAGAAAACGGGCATTCCTAGCCTTTTCAATAAAGCATTTAGTTTTGTTCTCATGAGATAGAGTCTCTGTCTGTAGACCAGGCTGGCCTCAAACTCACAATGACCTGCCTGCCTCTGGTTCCCAAGTGTTGGGATCAAAGTGTGTCATCACACTCAGCTGCCACATTGTTACCTCCCTTTGAATTACATAGTGATAACCTACTGCTGCAGAGGCACTATCAGAGCTAATGAAGTTTAACTATCTATTATTACTATGACTAGCTTCACTATTATCATTTGATTATCACATAACTCCACAATATAACAAAAACATATTTGTAACAAATATATATAATGGCCAGTAATCTCAGCACTCAGGAGGCAGAGGCTGTGGATGCCTTTGAGTTCAAAGCCAGCCTGGTCTGTGTATCAAATTTAAGGAGAGCCTGTGCTACAGAGTGAGACCCTGTCTCAAAACCAAGCCAAACCAAACCCAACAAGAACAACAAAAAGGGTTTTACTATGTACAGGCATTGTGTTAAGCAAAGACCCAGGCACCTCCATCACAATGGCTAATCCCTCCCAGACCAGTTTAGGCACGGATGCTGCATCAGCGTCACTCAGTACTCTGCAGAGTCTTTCCTTTCCTTCACTGCAGAAGGTCCTACCGGAGCAGCCTAGAGGGATCCGGGCTGTAGGGGCTTCTGAGTCCTGGTCTCTCTCACCTTATCCAGTTGACCTGATTTGTTGTATTTCTCTTCTGCAAAGACCAGCTCCATCTCACTCATGTCATTGTTGAGGATAAAGCACACCTTGGACTTATAGAATTCTGGGTCATCCGTTTCAAAGTACTGCGGAGATAGGAAGACACAAAGCACATGGACACCCTTTGCCATGAATGGAACTAACTTCTTGACACCATTTACACCTTCAACCTTAAAAATAATCAGCTCAGGGGGCTGGAGAGACGGCTCAGAGGTTAAGAACACTGGCTACTCTTCCAGAGGTTCTGGGAACAGAACCCAAATGGAGGCTCACAACCATCTGTAACTCCAGTTCCAGGAGACTCCATGCTTTCTACTCAGTGGGCACTGGATGTATCTGGTGTACGTACATATAAGCAGGCAAAACACACATACACACAATACAAATAAATCTTTAAAAATAAATAAATAAAAGGGGAAAAAGGACGTTAGAAAATCAGCTCATGTGGAACAATCCCAAGCTAGTCTGTCTTTAGTGAAATTGCATACCCACCATGTTACCTTGTAATGCATACGGAGTCCTATGATTTGGGCCAGGAAAGACCGAGTGAACCGGGCACGGACCAACTGCTTGTAGGCTCCCCCTAGAGAGGATTCATACAAACACTTGCCCACCAGCCGCCCTGCAAACTCATACATCTTCAAGCGCAGGTGAGCCGGGCGGTTAGGGTTAGGATGCACCTGTCAAAGAAACGAAAACGTCTTTGACCATTTTTCATCCATTCTAGCTAATTCTGGAAAGACTCCCAAATCCTATGCAATTAGGACCTAGAATGTCCCCCAAAGGTGTATATATTTAAAAAGTTTTGGTCCCCAACCCAGAGCACCGTCTGTAGCGTTCAGGCTACTGAGGTATGTCCTGGAAGAGAACTTCCAGGTCTCTCTCTGTCTTTCATGCCCTAGCCCTGAGGTGAGTATCTCTCCATACTCTGCCACTATGATGTGCTGCCTCACCACAAACCCAAAAGCAAGAAGGCTGGCCAGCCATAAACGGAAGCCTCTAAAAGTCTGTGGTTAAGTGACATGTTCTCTTTATAAGCTGGCTCTCTCGGGCACTTATTACAGTAACAGAAAGCTGACTCATACCCAATGCTAAGGGAATAAAATTTCAGTTTAAGGGTCACAGAGCAGACAACTGAGTGAATTAAAGTGACTGGAGGACAGCGGCCACGGAAGAACCTTAGATCTGTTGTGGTCCTTAGTTGATTTGAGTACAGGTGATCTGATGCCTGTCCCTAAACAAGAACTTTTCAAACGAACTCTACTTGTGATTCCTTTCTAGTCAGGGATCTAACACAGAATCCAATAGCCACAGGCGTTTTTCTTTGGATAATAGCTTAATTACTATAAGTGAGTAAAGCCACAATGTAACGCTCTTAGCCAAGTGCTCAACTCTTTCTTCCCATTCTACAGATGAAATAATGGAGCCAAATAAAAGGTAGGCAGCTTGACTAGCTCATTACACACAGCACATGAAGAGCAAAGTTGTCCAGCTTCCCAGGTCAGCTGTGCGAACCTCTGTGTATTCAGGGTAACTTTCCACCCCCACGGGGAAGGCACGGACTCACTAGTGCTTGGTTGCTGTCAGTGAACCGGGCGAAGAGCTGACTGGTGGTGTCAAACAGTGCTTTGCAGATTAGCTCAAACCATTCCCGGCGAGGCCCTCCCCAATCCAAAGCTGAAAAGTATAATGAAAATTAAAAGTGATGCTGGCTTACACCACCAATTATTACTTTTCTTCCTTTAGTAACTTGTAGCATTCTCCTGTCCTTCTCTCTAGTCCACTACCTGTTTCTTTAATGCAGCTCACTTATATGGTGAACACTTCTTGAAACCCCACTTCCTCCTATAGATTCCAAGGTATTCTGGTCACTAAGCTAACAGAAACACAGGTCAGAGATCCAGGAAATGGACACTGTGGTTCTGCAGTGGTTATTCTGTCCTTCTGGTGTGAACTGTAGGCGCTCACCCCCATGTCTCTGTTCTCTAAATTCCACCATGCCAGCCAACCAGGCTCCTTTCCTCACTCAGAGAAGCTGGGTCTTTGGGGGAGCTTGAAGAGACTGGTCTAAGCTTTTTTGGAGAGTGGAAGCAGGAAGGAGAATTAAAACAATAAAATGAAACTTTAAAACCCACCTTCCTCATCCTGGAAAACTACTTCAAAGTTCTTGCTCCAGTCTGAGATGGAAAAGTTCCGAGTGGCCTTCAAAGACTAAAAAGCAGAGCAAGGAAGGAAGCTGTAAGAACACTGGCTGCTCCCTCAGCTTCTAAAGAGAACCCTCAAGAGCAGCACTGCCCACTGAGCTAACCACTTCGTGGAAGTGATAGATGGCACACAAGTAAAACTCACTGATGTTATCTTATATTCAAATACACAGATCACCAGGAGAAATTCAGTATTTAAAAAAACTGGGGACAACCACACCAGAGACAACCCCAACTGGCTCCATGTAGCCAGGTGGGCAGACCACACCTAAGACAACCCCTGCCTGTTACTGGTAGGGCATAGCATTCCTGAGATGACCCCAGACACCCAGAATCCCTATGTGCCACTAAGATGAGCAAGCAAGTGGTGGGGAGATGGAAGAGAAAGAGAGACAGAGGGATGTGGGCACAATGAAGAGAGGTGGAACCGGAGATTCCAGGCACAGCCTGGTAGGAGTAGCCAGCAGTGCTCCCTAAGGCCAGGGTAAGATCCTGGCCTGTGAAGGGGGTCAAGACCTATAGATCCAAAGCTGCAGGATCTCCATGACACAGGGAAACAACAGGATATCCAAGAGGAGTCCCAGTGAGGATCCAGTATTGACAGTGGAGCAGAAGCCAGAGGCCTCGAATCACTGCAATGAGTCCCTGCAGTGAACATTTGCAAGTATAGTTGTGGACAAGGGGCTGGCAAGATGGCTCAGCCAGTAAGAGCACTGACTTCTCTTCCGAAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTTACAATCACCTGTAATGAGATCTGACATCACCTTCTGGTGCGTCTGAAGACAGCTGCAGTGTACTTATGTATAATATTAAATAAATCTATGGGCTGGAGTGAGCAAGGATTGACAGTGGAGTTGACTGGAGCAAGCAGAGGTCTTAAAAATTCAATTCCAACAACCACATGAAAGCTCACAACCATCTGTACAGCTAGAGTGTACTCATATACATAAATAAATAAATCTTTAAAAAAAAGTGTGGACCAAAGGGTATCCTGTGGGACACACTATGACACATTACAGCTTTCACAATGAGGTTTTTTTCTCTCTTTTTTCTTTTTTTTTGGAGTGGGGTAGGGTAGGGTGGGAAGTTCCAAGGGCAGAGGGCAAATACAAAGGGATGGAGAGAGGTGAGCGAGACTGGGGTGTATATGACCTTTATAAAGAATCAATAAAAAGCTAAATGAAAAATAATCTAAGCATGGTAGGAAATCCCCATGACCACAGCAAACATGAAGCTGAAGCAAGAGGGTTCCAAGTTCAATGCCAGCCCAGCCCACACTGGGGTAACTTCGCCCTGGACAAAACACAGACAAACAAAACCATCTAACCTAGATTTAAAAGTTAGAATTGTTTTATTTTTCTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCCTTCCTTCCTTCTTTTTTTTTTTTTTTTTAACAAATTTCATTTTATTAATGAAAAACTCTTACATAAAATTTTTTTGGGTGTTTTTTTGGATTCAGGCAGTAAGCTGTAAACAGAACTCATGAGTCTTGGTTTCCAGGGAAGTACAGACTCTCTGTTACTCATCTTTATCTCCCACAAAGCAAGGCTACATAGGGAGCCATGCCACATAGGGTGTGTGGCTAAGAAAGGGGTTTCCAGTCATAGCCACCAGAGCCAAACACCACACACTTTCTGGACAGGTACCAAGCCTTCTCACTCTGGGAGAATGTCAGGCCCAGGCCAGTGTCTGTGGTGGGGACAGACACTCATCAGGTGTTCCATTTCTACCAGACCAGAGTAACAATAAGTATTACTTCCTTCTAATCCAGCCGAAGTCCCTACACCAGGGGTGTACTATCAGAGTGCTTCTCCAACTACGACTTCTGCTCCCTACACTGAACTGTCCAGGAACTAATCAGCACCGACCAGAGACCTGATGTCTGTCAGTCTCCCCCACAGTTGCATGCCTCTAACACAGTGGCCCCAATTCAGTGTAATAAAGTATATACAACGAGAAGAACAAATAGTCTCTAATATGTTTCAAGGGAGAAATGTATGTTTCATTAAAAGAAAAGAACAGAGAAAGCACACACTACAGGATATAGTCAAATACAGAAGAGCTTCTTAGCTGCAGAACCTGCATAGGAAGACATTACATACCGACTCCAACAAGGCGTGTCTGCTGACCTTCAGAGTGACTTTGGAATGAGGTCTCTTCATATGTACCTGCCGAAGCTCTCTCTGGAAAAAGTTCACCTTGTCTTGAAAGGTCTCAGAGCCTCCTGAGGAGCAAGCAAGCACCAAGGTTCATCATGACTTACTCTCAGGCGTGCGATGGAGCACAGCTCTACAGGTACACGCCCCCATGGCTCACCGCCTTCCCACCCTTACCAATGTTCTTGTGCAAGGAACGGATGAAAGTGGCTGCTAGGATATTCCTTTCTTTACAGCTGAGCTCCACTGGAGGCTGAATGCCGTCATCCACCACCAGCGTGAGGAGCTTGTGAACAGGGTCAGGGCCAAGGTATGAAAACTGGCACAGAAAGTTTAGGAGAAAGCAGTTAGCTCAATCTACAGACCCCTTTATTCCATCAAAGCCTTTCACATGTCTACTCTGCTACAAAAACAAGGCAATCCTGGTTTTTTCCAAATACATACTTGCCAGGAATACATATCTGGTTTATCTTATATATTTATTTATTTAGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTGCCTGTGTGCACAGGTATGTGTGGGTGTGGGGTGTGTGGGTGGGGGTGTAGGGGTGTGTGTGGGTACATGGGCCACATGTGGAGGTCAGAGGACAGCTTGTGGGTTGGTTCTCTTCTTTCACCACATGGGTCTGGAAGATTGAACCGGCTCACTAGGCTTGGCTGGCACACTTAGCTGCTGAGCCATCTCATCAGCCCTATGCCAGTGTTTACAATTCAGTTTTCTGTTTATCTCTGTTCCTGTTTCAGTGTGCGTGCCCATGCTGTGTATAGTCTCAGGAGACGGTTCTGCCTTGCTGGATAAACACTCCAGGATGCCTGGCCCTTGAGCTTCCAAACGACTCTCCTGTCTGTGCCTCCTGTCCTGCAGTGAGTGCTGGGGCCATGTGCAACACACCTGCCCTTTTACGTGGGTTCTGGGGATCAACTCAGGTGGTTGGGTTTGGTCTACAAGGATTTCTACCACTGAGCCAACTTGCTGGCCCCTAGTTTGAATCAGAGAAACAGCCTTTAAGCAAGCATCAATAGGCAACATGCATTTCACCATAAGCGGTCAGATGGGGAATACAGCTATTATTCCAGACTTCCTGAAGAAAGTGATCTTTACAAACCACACATCCAGGTTAAAGCTTAGATTTAACCTAAACATTGCCCAGAGCAATTAAAAACAAAATCGAAAAAGACAAATTCTTTTAATCTATGAGAACATTCCTTCTCATAGAGTGAGCTCCAGCACACCGGAGACTATGAACAGGCACAGAAACCGACATCCTTTGAAAGTGGTGATGAAGACAGAATACACTGGGAACACATTCTGAACTGCTATTGGTACATACTGATAAAGTATCCAGTCTCATTACCACACTTCTTGCTAAGAGTTATATGTCCATGGCCTTTAATGAGGAGATAACCTTTTCTTTAATGTTCCGAAATACTGAAAGCCCTCAGGAAAAAAAGAAGACTGAGCAGCCCAGAGCCGAGTCTGCTCAGCACAAGGTCTGCCTTACCTTTGTTCCTGGGCACACTCGGAAGGTGTAGAGGCGCCAGGGGATGATTTTCAGGTAGAATTCCTTCACAGAGAATTGCTGGGGGACCACACAGAGAATGAAATGAGGGACAAAGAAGAGTGTCCATCCAGTCTGGGTATCACAATACAAACACCAAAGCTTTGTCTATATTTTCATAATAAGAGGATACTTTAGTCAGTCCTAAAGTTAAGTTTGCCCATGGAAGGTGAAGGTCAATCCTGACCTGACACCGTGATTTCCCAGGCACACGCCGTCCCAATGCTCTAGTCTGACAGTCACTGTAGAACGCCTGCAATTAGGTCTTCACTTTCTGGCACAAAACATTTCACCTCAGAACAACCAGGACCCTGCCTTGCCTGCTGGGGCTCAGTGACTCCCCAAGCCCCCACACAGGAGCAAACAGCACCCACCCCTATTGCTCCCCACCGACTTGCTGCTGCTGGTTTGGTTTGAGACAGGGTTTTATTCCCTAGCCCAGGCTATACTGGGACTCACTAGGCAGCCCAGGCTGTCCTTGATGCCAAAGTGACCTTGCTTCAGCTTCCTGAATGCTGAGATTATAAGTGTGAGCTACCATGTGTGGCTCTTTCTTCCTGTCTGAATGGTACTATGGATAGGGGGTTGTGATGGTTTGTATATGCAAGGCCACTACTTAGAGGTGTGGCCTTGTTGACAGGAAGAAGGTACGAATGTGCAGGGTGACAGGAAGAATGTATGCATGTGCAGAGAGGCCTGGGGAGAAGTAGAGTGGGGCTTCACAAAAAAATCTAAATACCGTCCGGGCGTGGTGGCGCACACCTTTAATCTCAGCACTCGGGAGGCAGAGGCAGGCAGATGTCTGAGTTTGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGATAGGGCTATACAGAGAAACCCTGTCTCGAAAAAACAAAAACAAAAAACAAAACAAAACAAAACAAAACGGGCTGGCGAGATTGCTCAACGGGTAAGAGCACTGACTGCTCTTCTGAAGGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCACCCGTAATGAGATCTGTCGCCCTCTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAATAAATCTTTAAAAACAAAACAAAAGTTATCTAAATATCTTACCATGACAAATTAGTTATCTAATTTCCTTACCAAAGAAAGCTCTTCTTGTGCCATTTATAAAAGATAATGCAAGATTTTTTTTTAAATCTGTTATGTAAATGTTATCATACACAGTTCCTTGTTTTAGTGCTTGGACTGCCCCTCACATGCCTTAGACATGTTCTGCCTTGAACTACATCTCCAGCCCCTCCATATCCTCCATGTCTTAAAAACCAATGTCCCTTTTTGTTTTGTTTTTTCAAGACTGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCACTCTGCAGACCAGGCTGGCCTCCAACTCAGACATCCACCTGCCTCTGCCTCCCAAGTGCTGGGGTTAAGGCGTGCACCACCACTGCCTGGCAAAAACCAATGATCTTTAAAGAAAACTTGCAGCAACACTAAAAACACTTTGCCAACTTTAAGAATAATTGATTCAGTGCTGATTGTGTGCAGTTCCCACCCCACTCATCACCTCACTGCCGTCCCTTCTACTCTACAGGAAGGCTCGATTTGTTAATACATATTTATCTACATTATGTTAGAAAGCACAAGGGTCTCTGAGTTCAAGGCCAGCCTGGCCTACAGAGCAAGTTCCAGGACAACTAGGGCTACACAGAGAAACCCTTGCCTCGGAAAAAGGAAGGAAGGAAGGAAGGAAGGAAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGGAGGAAGGAAGGAAAGGAGGGAGGAAGGAAGAAAGGAAGGGCAAACGAATGAGGGAATTTGAAGCCAGTCTTGATTGTGAGATTAAGAAATATAATAGGATTTCCAGTAATCTCATTTTTGTGAGGACTAGAAAAACACAAACAGTCAAAAAGCCTCTAAGAAGGTGTCTCTAAGTTTGAGACTTGGGAAGTTCACCTTCCTGTACCTCTGCACAGACCATATTCATTACAATCTTCAAGCCTGTTAGTGACAACCATTTAATGTGGTTTCACATATTCACAGAATGAAAGATCAAATTGTTTCCATACAGCATAAGGGACCAACAGTGATTTTTATCCTCACACAAAACAAACCACTTCAGCAGCAGCGGTGGGGACCTCCAGTTCCATGCATGCTAGGAAGCCTTTCTTACTGTCACAGAAACACTCACTACCTGGAGGTGACCCAGCCCTACTGACACCCAGGCTGTCACTTCAGAGTTTCCATTACTACACTGGCCAGACTGGGCAGGGTGGTGGTGGTGGTGTTTAAGGCAAGAATAGAGGCTCCAACCTTTGGTGACACATAGCAGTACACCTTCTTCGGTTTCTTCACCTTCTCAGGGGTGTGACACTCTGAGGGTGAGTCTTCATCGTCCTCCTCAATAGCAGTGGAGGGCCGGCGTTGAGAAGAGCTCATGTGCATGGGAGGAAGATGCCATGGTGTGCTGGTACAGTTGTTAGCATTGTAAAGGTAAGCCTCAAAGTAAATGCTCACTCCTGAGGTGGACACATTTCGTTCAACAATATTCTTCTCATTCTCTGTAAGGTTTGAAATAGGAATGAGGGGAGGGCAACCTGACCTTCTAATGGTCACTTCATGTACTACAAGGCAGTTAGCTCAAATATCAAATGTTTGTCGGGTGGGGTGGAGAACAGATATACAAAAAGGAAATGGCTAGAAAAAGACCAAGTCACAGCTTTCAACTCACCACTAAGGACAATAATGTCAAATTCGCCATTATTGATGGGTTGGTTCTGATATGAAATGCAGGCATGGAAGCAACCTCGGGAATGCAGGGTGAGCCGCAAGAACACCTGGCAAGTCTGCCTGTTGGACGTCACTGACTTCTCAAATGAGACTTCATTATTCTCTTCTTCTTGAGGACCAAGCTACAGGAGGCAAAGTGTGACGACACATGTATCACTTTCTGCTTCACCAAGAGCATCCCTGTGGCTCCTTTAACCTATGCCTCACACTTCCTAAAGGGACAGGCACTACCAGATACAGCCAAGGAGGCTCAGAAGTCAGGGAGGAGAGGTGGAAGTGAGGGCACCTGGAGAGCCCGACTGTCCTGGACGGGGAAGAGACTCACCTCGTGAATGGCTAAGCTGTAGCTGTGCTCATCTCTCAGGGACATGGAATTGTTGGTTGGGTTGTCATACTCATCTCGGGGCACTATCTGCAGAGTGTGTGGCTGCCCACACGTCAACACCAGAGTAGAAAAATGGCACACAATTTTTGTTTTGGAAGGAACCACCATTCCTGAAAACAAGATAATGAAGACCAATAAAATGCAGATTCACAGAATGAGCTGTGAAGACTTGGTTTTGTTGTTGCTTTGAGAAACGGCTTTGTTTTTCTTGGTATTTTTAACCACTTTCTGCACCAAACAAAATGAGAAAGAACTGCGAAGCACAACTTCAGCTGCAGTCCCTCAAAGAGCCTGGAGGAGCACCTTCAACCCCAGCACTCAGAAGGCAGGGGCAGGTGGATCTCTGTGAGTTCAAGGTCTACATGGTGAACTCTGGAACAATCAGTGTTATATAGAATGACCATATCTCAAAAAACAAAAAGCCAAAAAAGTGCCAGGAACTGGTGAGATCGCCTAACAGTGAGGCACCCTGCCAGTATGCTGACCTACGTGACTCCCAGAGCACAGGGAAAGGCGGAAGGAGAGAAAGACATTCCACAAAGCTGCTCTCTAATCTCTCTGTGCATACCTGCCATTGGAATGTGCCCACACTCATACACACACAGAAAATAATAGTAAACAAAATAAGAAATGAACACTGAAAACAGTGAAAGCAAAATCAAAGGGCGTGAGACAGGTGCACATCTAACAAAGCACATGGACGACTGCATGTGGAGCACCATTAATGCTGTGAAAAGAAAACTGATTTAAAAATACAGAATTTATTCTTTAAGAATTACATGGCCAGGCGGCGGTAGCGCATGCCTTTAATACCAGCACTTTCAAGGCAAAGGCAGGCAAATCTCTCAGTAGAAGACCAGCCTAGTCTACAGAGTGGGTTCCAGGACAGCCAGGGCTACACTGAGAAACCCTGTCTCAAAAAACAACAATAATTTCCAGAAACAAATTAACTATATTCTGATCTTACCCATCCACACACACCCAATCCCCATCTGCCCATCTCCACCACAGGTCCCTCACTACTCCGTGTCCTCCTTTTTAACCCAGAGTCCTGTCCTTGTGCACGGGTGAGGACATCCAAATAACTACGATACCATGCTTGTGAATTAAAGGATTCAACACAGAGACATGCCAGCTTGCCCATGTTGAAACACAGGTTTAAGACAATTTTTAGTAAACTCTTAGGAAAATATTCACAGAATTAGACATTATACCAAAATCTGTAGAGAAAGGAAAAGGAACTGGAATTAGCTAAAGCAATTTTGAAAAAGCAGAGTAAGTGGAGGAGAGTAGTGCCTGTGATCTGACGGGCAGTAAACAAGGCCCTGGTACTGAGGAGGGGGCAGACACAACAACCAACGTCATCATACAGAACCTCAGGCAGACCACCACACCCACCCACCCAGCTGACGAAGGTGCAAAACGTCTATCCATGAAGGAAAGGCAACTCTCTCAACACATGGTCCTTGGGCAACCAGAGAACTTCGAGGAAAAAAAAGATCTATCAAGAACCTTGTCACAAGTGTCACATGCAAAAGTCAACTAAACATGGAGCATGGGTTTAACACCAAAGTGAAACAATAAAACTTTAGAAAAACCAGTGGAGAAAAGTCTTTAGGATCCAGAATTAGCCAGAGACCTTTGATATCAAAAGCATGATCACGAAAGGAAAATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACTTTCTCAATATTAAAAAGGGTTTTACACCATGGAAGATCCTAAGAGGACTAAAAGAAAAGCAGGATTTTAACTGTTGGACAAGGGACTAGAATTTATGCATAGAATTTATGTAAAGAGGCTCTCAAAACACAACAGTTGGAAACCCAAATATTTCTAATTAAAAAATACGCCAACAACATGAAAAGATATTTTACCAAAGATGATAACATAGCTGTCAGATGAGCACATGGAAAGATGTTATCACCCCTTACTATTTGGGAAATGCAAGTTAAACCTACACTCATCAGAATGTTCAAAATAAAAAAGTGACAATATTACAGTGGCAAGGAAGTGAAGAAATAGAGTCCAAATGCTACAGCCACTCTGGAAAAGAGTTTAGCAATTTCTTTCTTAAAAAAAATAAAAATGCAACATCAACTGTCTTGGCAAACATCTTCCAGAGCATTTAAGAAATGCTCTTATAAAAAAACCTATACATGAGTGTTAACAGTAGCTTTGTTGATAATAGCTAGGAACAGGAAACAATCAGGATGACCTTCAATGGATGAATTGTTAAACAAATACCCATACACTGAATGGTACCCACAAAACAGAACCAAGCACTCATACACTTAACAACATAGACAGATCGCCCGAAAATTCAGCCAGTTTTGAGAGGTTACTTCAAACACTCTTCATATGACTTAGTGGAGAACTATGGTTTGGGGCTTTGGTTTTAGACAGGGGCTGTGCATCCCTGGCTGGCTAAGGAAGACCTTGAGCTCCTGGTCCCCCTGCCTCTACCTCCCAAGTGCTGGAATTACAGATCACTGCAGACATGGGTCACTGTATCTGGTTTTTGTAAAAGTAAAGAAATGGAAAATAGAAATGCATGAAATTCAAGAAGAACGAAGACCAAAGTGTGGACACTTTACCCTTTCTTAGAAATGGGAACAAAACACCCATGGAAGGAGTTACAGAGACAAAATTTGGAGCTGTGACGAAAGGATGGACCATCTAGTGATTACCATATGCAGGGATCCATCCCATTATCAGCTTCCAAATGCTGACACCATTGCATACACTAGCAAGATTTTGCTGAAAGGACCCAGATATAGCTCTCTCTTGTGAGACTATGCCAGGGCCTAGCAAACACAGAAGTGGATGATCACAGTCAGCTATTGGATGGGTCACACGGCCCCCAATGGAGGAGCTAGAGAAATTACCCAAGGAGCTAAAGGGAACTGCAACCCTATAGGTGGAACAACAATATGAACTAACCAGTACCTGGGAGCTCTTGTCTTTAGCTGCATATGTATCAAAAGATGGCCTAGTCAGCCATCACTGCAAAGAGAGGCCCATTGGACTTGCAAACTTTATATGCCCCAGTACAGGGGAACGCCAGGGCCAAAAAGGGGGAGTGGGTGGGTAGGGGATTTGGGGGGGTGGGTATGGGGGACCTTTGGGATAGCATTGAAAATGTAAACGAGAAAAATACCTAAAAAAAAAAAAAAAAAAAAAAGAAATGGAAAATAGATCCCTGAGTTTCCAGGACGTCAGAAACAAGCCTGGTGGGGGTGGGAGAAAGATGAATGTAACTACAAAAGGGCAACACTGGAAAGATCTGTACAATGCCAGAAATATTTGTGTCCTTTAAAAAGCTTTTTAAGTTTTACTTGTATTTTATTCATATGTGTGCTATGCCTGCATGTATGTGTGTGCACTATGTACACACCTTGTGCTCATGGAGGCTAGAGTTACAAGATGGTTGTGAGCCATAGTCTGGGTGCCAGGAACTGAACCTGGGTCCTCAGCCAGTGCTCTGAACTGCTGAGTCATTTCTCCAGCCCCATGTACCTTAACTGTATTAATGTCAGAAACATGGCTGTGGTGCTCTGCCATATCACCATCACGCTACAATCTGAAAGATGTGACCACTGGAAAGCTAGATAAGGGTATCCCCTATCTCTTATCTATCATGCATGAGAATATAATTCTCAACATAAGTTTAATTTAAACAGAGATGGTACATAATGATACAGATTGTTCTCCATTGGTGGCAAGAAGTATGTGGCAAATGAAGTGGACAGTCTCTGAATGAGGCATTCAGGCAAACGAGACTGATAATGCAGACAGCTTGGCCAGTTTCCTTTGCTCTAGAAAAAGAGAACTGGGCACCAGCCAAACAGAGACTCCAATCATTAGGATTTAACTCCCAAAGTTAGCCAATGTCCTATTATTTCTGTGGATACCAAACTAGCCAGTATCTAATGGCATCATCAAAAATGCTAGAAAGGTACAGGCAGATAGGAGTTCCAAACACTAAACACTAATATTGCAGAACCAAGAGTCAAGAGTCAGAAATTACAGTTTAGACTTCAGTGCCAGTGAGCATTTGTTATCTGGCACAAAGGAAGGAATCACACATGGACAGATGCTCTTCGGGATGAAGTAAAATACATATTGACAGTACTAGTGGGTTTGGGCCAAGGTGCTTTTACTTAAAAAGGTCTTTAAAAAAAATCAGAAGAATGTATCTCCTTTCTATTCTTGTATCCTAGGCCCTCCAGAGCTATCAGACTGACTACTCAGCAATTCTATTCCTGGAGAAATCCAAACACATTGCCCACACAGAGACTCAAACACGGAGGCCGGAAAGACCACAAAAGCGATACTACTTCTCTTCTTTGGAAAGAACACATTGCCTCCAAGCCTGCTGGCCTGCGTGTGATCCCCAGAACCCACACGATGAAGGATAGAGCTGACTTTACATGCTGTCCCCCGACCTCCACATGAGAACTGTGGCCTGGATCTGCCCCTCCCCCAATAAATAAGTCAATAAATGGATCCTGGGTGGGTAGATAGATAGAGAACCTGTACACAAATGTTTACAGTAGTCAAGAAGTCACATGGAAAGAAACAACCTAGATGTCCACCGCTTACAAAAAGACAAACAATACACGGCATGTTACTTGGCAAGGAAAAGGGATGAAGGCCTAAACATGCTACAGCACAAGGGACCTGAACAGTGCTCGTGAGGTCAAACAACAGTCAAAAGGCCTCGTAGTTTATGATTACACTGATAGAACTGGCCAGAGAACTCAACTTCACAGCATCTGTAGCTAGGGGTTTGGCAACAAAGTTGCTAATGGGCCTGGGGTTTCTTGGTACCATAATGAAAATCCTCTAAAATGAACTGTGGTGATA
GF3 -1 -1 1 GAAGTAAGTTGTGTTGAGTAGTATGTTCAC
GF4 -1 -1 1 CTCTTGAGTTATATGTGGCTCACTGGCTG
EX52 -1 -1 1 GCCTGGTAGGTTCGTGGTTCATTC
EX32 -1 -1 1 CACGTTAGAATTCGGCTCCTGGAG
GR3 -1 -1 1 CAAGTCTGCCTGTTGGACGTCACTGACTTC
GR4 -1 -1 1 CAAGTCTGCCTGTTGGACGTCACTGAC
Comments:
Created
Target: 2803Ensembl ENSMUSG00000042350 -- 86281110 -- 86281347 ENSMUSE00000270522 -- ENSMUSE00000270522
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:12:1:121257530:1, start 86281109: end 86281347
Found 6 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
REJECT (1) gap pair G5:12:86286847:86287846_753 G3:12:86276610:86277609_561
REJECT (2) gap pair G5:12:86286847:86287846_753 G3:12:86276610:86277609_902
REJECT (1) gap pair G5:12:86286847:86287846_753 G3:12:86276610:86277609_554
ACCEPT (0) gap pair G5:12:86286847:86287846_152 G3:12:86276610:86277609_561
REJECT (1) gap pair G5:12:86286847:86287846_152 G3:12:86276610:86277609_902
ACCEPT (0) gap pair G5:12:86286847:86287846_152 G3:12:86276610:86277609_554
ACCEPT (0) gap pair G5:12:86286847:86287846_144 G3:12:86276610:86277609_561
REJECT (1) gap pair G5:12:86286847:86287846_144 G3:12:86276610:86277609_902
ACCEPT (0) gap pair G5:12:86286847:86287846_144 G3:12:86276610:86277609_554
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

webmaster@sanger.ac.uk

Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK  Tel:+44 (0)1223 834244