High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by dk3
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 174960
Chr: 1
Strand: 1
Status: Ordered
BACs: RP24-572K23 : RP24-144F16 : RP24-392P22 : RP24-303M10 : RP24-104K6 : RP24-70I24 : RP24-331L1 : RP24-231G17 : RP24-210F11 :
Instance: Plate 184: Well F01: BACs RP24-392P22 RP24-144F16 RP24-303M10 RP24-572K23
Oligos on GRCm38:
Type Start End Length Seq
G5 86299035 86299084 50 TAAAGGAAATTCCAAGAATTCAACTAGACTCTTATGTGTAAGATACCCCA
U5 86304528 86304577 50 TTCGAGACTTCCCAATTAGAGCAAGAAAGGCTCGCTTCTGTTGAGTCAGT
U3 86304689 86304738 50 CCACATATGTATGTGAACTAAAAATAAAAACCAGAGCTCAGGGGATGGAC
D5 86307447 86307496 50 AAGTATCAATTAGCAAAAGGCAAGAGTTCAGGCCCAAGGCTAGTGTTCCA
D3 86307543 86307592 50 ATTGAAGTAGACTTGGCTGGAAGGAGAGCCCAACACATGCAGGACTTTTG
G3 86311415 86311464 50 TGAGTGACGGCATTTGGAGAATCTCAGAAGCATAGCACCATAATTATTTA
Oligos:
Type Start End Length Seq
D5_D3 88204072 88204117 46 GTGGGTGCCCTTAGGCTCACAGGCTCCCTGATGTCCTTCGGAAAGC
G5 88195610 88195659 50 TAAAGGAAATTCCAAGAATTCAACTAGACTCTTATGTGTAAGATACCCCA
U5 88201103 88201152 50 TTCGAGACTTCCCAATTAGAGCAAGAAAGGCTCGCTTCTGTTGAGTCAGT
U3 88201264 88201313 50 CCACATATGTATGTGAACTAAAAATAAAAACCAGAGCTCAGGGGATGGAC
D5 88204022 88204071 50 AAGTATCAATTAGCAAAAGGCAAGAGTTCAGGCCCAAGGCTAGTGTTCCA
D3 88204118 88204167 50 ATTGAAGTAGACTTGGCTGGAAGGAGAGCCCAACACATGCAGGACTTTTG
G3 88207990 88208039 50 TGAGTGACGGCATTTGGAGAATCTCAGAAGCATAGCACCATAATTATTTA
G5_U5 88195610 88201152 5543 TAAAGGAAATTCCAAGAATTCAACTAGACTCTTATGTGTAAGATACCCCAGGCTCGGAGGAGTCTCCCTGCTCTCCCCTGCTCTTCCATCTCCTCCCATCTCCTCCTTGCTCCTTCTTGGGCCCCTGACTTACAGAGAGGACCTCTGTCTGTCTGTAAGATGGTTCAGCTGTTATATCTCACCTGTCAGGTTTATGTCATATACAGACACACACACATACCACATACATACATACATACACACACATACATACACACACACATATATACATACACACATACATACACACACATACATACACACATATATACATAAACACACATACATACACACACTAAATATATAAGTGTACATTTAAAATTGAAATAATAAAGGGGAGCCAGCCTCCAAGGAAATACGTCCCCTCTGTTCATCCCTTAGCATTCCCCTGCTCCAGATCCTGCCTCCCGTGCCTCCATGTGGGGGAGACCAGACCATTTACCTGTGGGGACTCTGCCAGTGCTGACCTGAATAGCATCCCTGGGTTCTCCCTGACCCCTTCAGCACCACCCTGTACTGTGTCAGGTTCTCAGTATTGGACTCCTATGAGGACCAGAGGTTCCACTCTCACACTTTTGTTTGTTTGTTTGTTTGTTTTTGCTAGACAGGGTTTCTCTGTGTAGGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGTATGGCCTCAAACTCAGAAATCTAGCTGCCTCTGCCTCCCAAGTGCTGGAATTAAAGGCTCAGTATGTGGGCACACTATCACTGTCTTCAGACATACCAGAAGAGGGTATCAGATGCCTGTTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACCCGGGTCCTCTGGAAGAGCAGTCACTGCACTTAACCACTGAGCTATCTCTCCAGCCCTCATTCTTAACACTTTCTCATCAGATATCATCTCAGACCCAGACTTACCCACTGCAAACTTCTAGAACAAAATGCAAAGCAGGCATCCTACCACGGTTCTGAGCTTGGAGAAACTCCTTTCAAGGTCTGCATAGGAAAACATGTCAGGGCAATTCTCAAGATCACCCTGACCAACAGCTAGCCCTGAAGTCACAGAGGACCCATCATAGAAAGTTCCAGACCAGGGTAGTGCTCCACAGAGCTGGTCTGGGATGGACAGAGGAGCCATGCAATTTTTTTTCTTGTCAGGCAACTCTTTTCCTACCAGCCATTGACCTCTGACTGCCCCAGCGCTGCCTCACTGGCCTCCAGCTGTGAGTGTAGACAGCTGAATTTGAAGCCCCAGCTGTGGCTACCCCTGGAATGTAACACTCACTAAAACTAATTCACATGCCCTCAACTATTAAAGTGACTGCAGCTGACAGGGCTTGTTAGTCCTTCGGCCACTAGCTCCAAGTCTCTGCCCATGGCAGACTCTCTCCAAGGTTCCCATGGTTCCTTACTTTCTACTTCCCTGTTCTTTCTGACCCAGGGCAGCCTCTACATTGTCTCGCAACAATATCTACCCAGTATGCCCTCTCATGGGTCAGTTCTCCCTCCATCTTAAGTCTCTGCAGACCTAATGGGGAGGGAGAAGTCACAGGGAGAGGGAAATGGGAGCTGGGGAGAGCAAGTTATCCAAAGAAGGAGTGGGAAAGGAGAAAAGGGTGTTTCTTGATGTACTTCTATATGCTAGGTCACTGAGCACCCCTTCTGGGGCAGCAAGAGAACGTTCAGAAAATGACTTGCTGGTTTAAGGACTCTATTCAATCCCTTCTTCCACCCACACCAATGCTTCCTGGAACTGCGGTCTCCTCCCACTGAGCTCTCACCACAGCATGGGGGGCAGTGGCAGAGTCCACTGGCAGACTGGCCTTGCTCAGCCACTCCAGTTGTTCTGAGACAGTCCCTAGCCGGCTGCTATGCCCTCGTGGCTGGACAGGTGGAGGGAGTCAGACTAAGGGAGCCCAACGGTGGGGGTGGGGAGTCACTGTGGTTCTGAGTAGCAATGGGGACCTGTGGCTCAGTGAAAGAAGAATAGGGTGACATTGGAAAGTGACCAGAGCCTGGGGAACACACACGCACACACACACGTGCAAGGACTCATGTACTTCATTGTTACACATTTCCCCATTCTGAATTCACAGACCAAAAGATCTTTGACAAACTGACCAGTTCAACTGAAAAACCCAAGGTGACCTTGGGCGGTTCCCCACTTCCCCTGCTTCCCTGTTCCCTTGGGATCCCCTGGGATAGGTCAAATATTAGTGATCATAATATCAAAAGCACCCACCTGTCATAAGGCTCAGCGTGTAGGGGACACTGGTGTGTCTGCTTCCTCTGTCATCTCACATAACTCTGCATTGGCAGGAAATTGAGGTCTCAAGAGGATAAGCCAGCTGCCCCCCAGCAGCAGCCAGGAAGTGGGGAAAACTGGCCCAACCCCCAACCCTCTGGCACTCAGCCCTTACCAGAAACACCTGTGTCCACCTTCACCTACACCTGGAGAAATGCCACACTGGGATGAAGGGTGAATCAGAGGCCAAGAGGGCTGGTCTCCCCCAAGGCACCTTGTGGAGAGCTGAGACAGAGCACCTGCTCTTCCCCAAAGTCACACTTACAGGGCAAAAAGAGAGACGCCCAAAGCCTGGGTGTGCCAGCTGTGTTGGCACATTTATAACCCCCATCGTTCAGAAGGCTAAGGCAAAGTGATCTGGAGTTCAAGGCCAGCCTGAACTGCATGCCAAGACCCTGATTCAAAACAAACCACAATATCCTGGGTGAAATAGCAGAGACAGGGATAGGTAGCCAGTACCAAGCTAAGACAACCAGGACTGAAGACTGAGGACCGGTGTGACCTGGGACTAGTGGGACTGCAGTGACTCCTAGGGGCAGGCAGCCAGGCCTGTGAGACTTACACTTCAGGCCAGGGCTTCTTGAGGACTTAAAAGGGGCATTGTCCCAGGATTCTCCTCTGAATTTTTCATCTGAAGGCCCTTCATCATTTCCGACTGGAGCTTACAAATTCCAGAAAATTCCACTATTTGTTTAAGCACACACCCGAGCCTAAGGATCGCTAAGGGAAGGACAAAAGTAATCTTCACCCTTAGGACTCCCTGGGACTTTCTGTCTCCCTGTATTCCCTGGGAGAGGAACAGCATTGCAGAAAGGTATTGAGTTCCCCAGTCCTACTATCTGATAGAAATGCCTGAGCCGGGACTCAGGGACCACCTCCTGAGCCAGGCAGACCTCCTGGGTTTGAATAGTAAGGTTGTGACTTCATCTAAGGGTGTGTGTCCCTGACTCTAACTCCTGGTAAGATAGGAACAAACCATAGCACACGTCTCCAGAACCAAGTGAGCCACTGATGCCGGGGACCTAGAACCAGGCTTGACAGATAGGAAGTGCTGGAATGTAAAAAGTCCTGTGGGTATTTCTGAAGCGACCCCAGACCCCCAGACATCTGGATGACAGAAGGACCAGTAAGCAGCCTTATCTACCAGAAATGTTCCCAAGTCACCCAGAGGAGGCATCAAGGTGTCCCCTCCCTCCCTGTACTGTGATTGTGGTGTGTAGCCCACTCCCAAGAGAAAATGGTGCTCACACCGCAGAATAATAAAAACCTGGAGACAAAAAAAAAAAAAAAGTGGGGGTGCGGTTAAGTGAAGGATTTGTTGGCAATACAAGAAGGAGGTTTTTGTTTGTTTTGAGACAAGAGTCTCATTAGGGAGACCTTGCTGGCCGGGAACTCACCATGTAGACCAGGCTGGCCTTAAACTCGCAGAAATCCACCAACTGGGATTAAAAGCACATGCGAACACGCTAGCAAAAGAATGGACTTTTTGCAGAGGATAATAAGCCTTCTCACTGGGGGGTGGTAATGCAGAAAGAGTGGGAAACCCAGAGCTGGATTGACCTGAGCTGGCTGGGGGTCTAACACAGGCAACCCAGCATCATCTTCTCTTGAGATCGCCGCCCAGGCCTAAGCGAGCCCGGGAAGAAGAAAAGTAGACAGGGCAGAGATAAGGTAACTTTAGGGAAACAGCCTTTATCCACCTCCCTCCACTCTACACACCCAGGGCTACTCCAGAAGGAAGCAACCAGGCCGCTAGGCGCCGGGTGGGCGTGGAGGTGGGCGGTGTCCGACGTCCCCACGTGTCCCGAAGGAGGCGAGGCGGGCGACCCCGTGAAGGCCCAGCAGCGGGGAAGTCGCCGCCTCAGGATGTTGGCGATCTTCAAGGTCTCTGAGATTCTGGCTCTTCCCTTCGCGTTCCCGTGGGGCGTGCCTCTGAGGTGGCCAGGGCTGAACGGAGTTGCTGTCCCCACAGTGAGCCGGGGCACAAAGGGTTAACCTACCTGGGCCACCGCCCCTGAGGCCCCTCCTCCAGCCGCAGGGAAAAAAAGAAAGTCTCAGCGGGGGTGCGGCTCGGCCTCGTCCCGCTGTCCCCAGCCGCGGCGCTCAGAGCTGTGATCCGTGCGCCCCGCTCAGCTGCCCTGGACCGGGCCGCCATGTCTCTGTGGTGAGTCGGGGTCCCGGTGGGGCGCAGAGTACCTCCGAGCTTCACCTCCTCCCGGGGCCGCAGACTGCAAGGGATGCACGCTAGGTGTCCTGAGGCCGACCCCAGTTTCCCCGGGCAGCGAGGGTGGCGGGAGATGCGGAGCAGGGCGCGCCGCCGGAGCGGCAGATGGGATGTGGGAGATGCGAGGCGCACGCTTGGCTCTGCGGCCACGACTCCGGCAGCCTGTATGCAACGTCTCTCTAGCCTTGGGGTTTCACTAGCAGACACACACACACCCGACCCCTCCGGTCCCTCTTCCCTCCTGTGGCCTCTTCTCCGGACCGCCTCGACCCTTCTCCTTCCCCGGCTTTGCTCTTACTAATGGAGTGGATTGGTATGTGCGAGCCAGGGCCCTGAGGCTGGTTCTTACCCGGACTCCGGAGCTACGACGTGGAGCTTCTTTAGAACCCTGAGGACTGGAGTACTGGGTTCAGAATTCTCTTACTTCCATTCCCAGCAGCGCGCCTCCAGTCATTTCTGTCTTTCTCCATCCAACCTGGTAACCAGGAAGTCATGGGGCTCTTGAGGAAAGCTCGATGAATGTGGGTAGGAGGACTTAACATTCACTCACCCTGCGCCTCCGCAGGCTTAAAACCTGGCGTTATCTACTACGTGCTTCGTGGGCCCCTGGCTCTTGTACCTCCCAGACTTGGAGGTGAGAACCATCTCTCTGCTGGCCCAGTCCTGGAAAGTCATGAGTCTCCCAGTGTGAGCTAAGGAGGGAAGGTTCTTGGGGAAGGGGGCACAGGCAGGATCATTCCTGAGAAGAGCTAGGATTTCTGCCTCTGAAAAGGGTGTGCTGGGCTTACTTGACCAGGTAGGGGCCCCGGAAAGTGGGTTTTCGTCCCCAGGACTGAAGGGCTAGGCACCTCATTGCTTTGTGTAGAATATAAAGGTAAGGATAAGGAGTCGTGATGGGATCTGTCCACTTCCCTTCGAGACTTCCCAATTAGAGCAAGAAAGGCTCGCTTCTGTTGAGTCAGT
U3_D5 88201264 88204071 2808 CCACATATGTATGTGAACTAAAAATAAAAACCAGAGCTCAGGGGATGGACAGTGGTCTCTTTTCCTGGCAAAATGACAGCATTTCACTGGGTTCATGCCTGGTACTTTATCCTGTGTTCTGTCCCATTTTGGGGGAACTTAAGGGGTTAAATGGCCCCCTTCCCAAAATTGTTGTTGGGTTTGGGACCCCAGTAGCTGAATACACAGTGCTTCTTGAGTTGGGCAGTTAGCATGAGAAAAGCAGCAGGTTCCTCTGACCTTCCAGGAAGTAGAGTCCACTGGCCCATCTATGGGCCCCTAATCCTCAGGAGGAGTATGAGTGCCAGCTCACCTTCCTCTCTGCTGCTCCCCTTTAGGAAGAAAACCCTCTACAAGAGTGTATGCCTGGCTCTGGCCCTCCTTGTGGCTGTCACCGTTTTCCAGCGCAGTGTGACCCCTGGTCAATTCCTTCAGGATCCTTTACCGCCCACGCCGGGGCCAGCCAAAACTGGAAATCTGGTCAACCCCAACAGCTTCTGGAAGAGTTCAAAGGATGTAGCGGCTCCCACTCCCACAGTTCCTCGGGGACCCCAGGTCTGGGATGTGATCACCACTAACTGCTCTATCAACATCAACCTGACCCATCAGCCCTGGTTCCAGAGTCTGGAGCCACACTTCCGACAGTTTCTAGCCTATCGGCATTGCCGGTATTTCCCCATGTTGCTGAACCACCCAGAGAAGTGTGCTGGCGATGTCTATATGCTGGTGGTTGTCAAGTCGGTCATCACACAGCATGACCGCCGAGAGGTCATTCGTCAGACCTGGGGCCACGAATGGGAGTCAGCGGGTTTGGGCAGGGGTGCAGTGCGGACTCTCTTCCTGCTCGGCACGGCCTCCAAGCAAGAGGAGCGAACCCACTATCAGCAGCTGCTGGCCTACGAGGACCGTCTCTATGCTGACATCCTACAGTGGGATTTCCTTGACAGCTTCTTCAACCTGACCCTCAAGGAGATCCACTTCCTCAAGTGGCTCGACATTTACTGTCCCAACGTCCCCTTCGTTTTCAAAGGTGATGATGATGTCTTTGTCAACCCCACCAACCTGCTCGAGTTTCTGTCTGACCGGCAGCCCCAGGAAAACCTATTTGTAGGTGATGTTCTGAAACATGCTCGGCCCATCCGCAAAAAAGATAACAAATACTACATCCCAGCCGTCATGTATGGTAAGGCCACCTACCCGCCCTATGCTGGTGGAGGGGGTTTCCTCATGTCTGGCAGCTTAGCTCGGCAACTCCACCATGCCTGTGACACACTGGAACTCTTCCCTATTGATGATGTCTTCCTGGGCATGTGCTTGGAGGTGCTGGGAGTGAAGCCCACAGGCCACGAGGGCTTCAAGACCTTTGGCATCTCTCGGGTCCGAAGCAGCCGGATGAACAAGGAACCGTGCTTCTACCGGGCCATGCTTGTGGTACACAAGTTGCTCCCTGCCGAGCTGCTGGCCATGTGGGATCTGGTGCATAGCAATCTTACCTGTTCTGTCAAGTTCCAGGTGCTCTAATGTTGCTGAGCCACCAGTGCGTTTGTCCTCGGGGGATACATGGCATTGTCTTTGGTCCCCTGCAGGAGGTGAAGGGTTTGGGCCCTCTGTGCCCCTAGGTGTGGTGAGGTACAGAGATCCAGAGAGGGACTCCCAGAAATTCCCAGAAAGAGGTCCAGGAATAGCCAGAACTTCCCTGACTGGAGGTCATCCGAGAACCTGGATCTCTCCTATCTTGGGCCTCTAGCCTGACCTCTCTAGCCTGGTAGGAGGCCCTGGTGGACTCTAAAGGAACATTTGCTCAGGTACCTGAGCTAGACCTGGCCCCAATGGGTCTGTGGCTGCTTCTTGTCCTCACAGGGAAGCCCCCTTCCTGTGAAATGCCTCTGGCTCCCTGAGGGTCAAGCAGCCTTTGTGGGAGCTCAGGCCTGTGCAGGTCCACAGGTGTCCCCTGGCCCACATTGCTGGACCTCCTAAGATAGGAGCTCTCTCTCCATAGGCCCTCAGCACAGCCCCAGGCTTGCCTGGAGTCAGCTGCCGAGGCATGGCTCCTGGCTCTGACTTCTCTTGTCCAGAAGCCTGACTGGCCGCTCTGACTACCTCAGCACTCCTCTAAACCTCAGTGTGGGCTAGTGCCCCACCTTATGCCCCTGACACAGAGCAAAATAGCAGTGCTCCTGCACCCTGCCCACCCCACCCCCACCCCCACAAGGCACAGTGATCCTTGAGCATAGTCCAAGCCAAGTCTCACCTTTGGCACCTCACCAATACTGACTCCCTGGCCAGAAACTGGCCGGATGGGCCCTAGATGTAGACATTCTTCTATTACTGTGAAATTTTATTTATGAAGAATTGGAAGGAGGCCTTGGGAGGTGTACCTCTTTCCTGTACACTTTCCTTCAGCAGCTAGCATCTTTACCACTCACCCCCTGCCTTTCAAGCCCTCCCCCACTGACACCCCTACTTTTCTGGGGCCTGCCCAGATATTTTCCCCTGTGAAAGAATACCACCTCCCACCTCTGGCTGGGGTCACCTGGAAAAGATGGAGGCATCTTCTCTATTTTCTCAATAAAACAACTGGTTGTTGAAGGTGTCTGAATTAGCTGCTTCCTTGGTCTTGGCTAATGGGCGTGAAAGACCTCTAGAGGGGCCTTAGAGGAAGTGGGGAGCTGTGGTGGTGCAAGAAGCCTGCGAGGCTCTAACACTCAGGTGACCACATGGCCAGCTGAAGCCTTAGCCTAAGTATCAATTAGCAAAAGGCAAGAGTTCAGGCCCAAGGCTAGTGTTCCA
D3_G3 88204118 88208039 3922 ATTGAAGTAGACTTGGCTGGAAGGAGAGCCCAACACATGCAGGACTTTTGCCAAAGCTGCTGCTTCCGGAGAAAGGAGAGCGGTTACTGCCAGGCCAGGATCTTCTGAGATGGTAGTGAGCAGGCCAGCTGTGTCCAGCTCAGAGCGCTCCACCATGGGCCCAGCAGCTCCGATCCAAGTCCTAGGGTTGGAAAGGACAGGGCTGCCACCATTTCTCCCTAGCACAGGTCTTCCCACACCTCTCTTTGTTAGCTGGCTATATTATGCGTAGAGCTCAGTGCAAAATGTATGCCTAGTTCCCAGAAGTTAAAAACTTTAAGATTGCAACAGTGCAGAGCCCTTTGGAGGAGCCAGCCCCCAAAACAGCAGCAGGAGAGCTAGAAGGGCCAGACAGGCTCAGGCTGTGAAGCTGCATCTAAGGACCAGGGAGAGACATGATTTAATCAAAGGTCAAGGGAGAGAGATGGATGCTTGGTTGTGCAGCTAGGTGGCAGGCAGGCCTGCATCTCAAGCCTCGGAGGTTTAGGGAAGTGTCTGGAGCTGACCCTAAGAATGGGGCAACAGGTCAGTAGTTCTGAAGCGCCTACTTTGGCTTCCTTTGTCTTTGTCTTGGGTCCTGTGGACTGGGAACTCAGTGAAATTCGCCCCTGTACTGTCGGCCTTGATAACTAGTGATATTATCAAGACTCAGGCCAGTCCCTTCTTTCTGAGCCTTGCCTACTGAGCCAGAGTGTGTGAGGTTTTTGCTTCATTTGCCCAAGGCTGGGCTGGCAGGCCACAGTAACTCAGCAGAAGGCCAAGGAGCCCCTCAGGGGCATGCTGTCACACTTTCCATCCCCCTGCACCAGCCCTGACTGAGCCTTGTACCTGCTGCTCAAAGGACTAGGGGTATCCCCCCCCCCCCAGGTAGACTGGAATGTGGGGAGGAGCCACTATGGGGTGGGGGTAGGGAGGGGAGACAAAGGGGAGGTTCCCTGCTGAGTTTGTTAACAGATCTCAGCTCTTTGGTCTGGAAGTGGTAGAAACATACCAGACCCAGAGATACTCAGGCAAGGCTTCTCTGTTAAGACATGGCTCCAACCAAGAGCAGGTTTAGGCCAGTGGTTCCTAGAACAAAGGAAAGGGGTTACTCTTACAATTAAAAAAAAAAAAAAAAAAAAAAAACTCCAACCATCCATCCCATCCAGGTTATCTGAAGGGATTCAGAACCAAAATGGGTATTGTCTAGTCTTGACTGGTTTGTGTCCCCGTCCTCCCTGTAAGAGTAACAGATTGTAGAAAGCTCTGGTCCTGACTGGTCAGGGGCTAAGCAGTCTCATGCAGTTCGCTAAGCAGCTTTGACTCTCAGGGTTGGGTATATTCTCTGGAGAGTGCCTTTATCCTACATCTGAGAGAATGAATGATTGCGTTTCTCCGCTCAAGGCTATGTGCGGGCCCTGGGTCACTGTTTGAATCTTCGGATAGAGGACCAACCAAACAGGCAGGCTCCTCCCTAAGGTCAGATGGGCCTGTCTCTTTTAGGTGGCCCTGTCTGTTTCAAGTTAGGGTGCTCTCTGCAGGCTCTAAACTATCAGAACACACCAGGGTCCACATCCTGCTAGTCCAAGCAGGACAGGACTCTCTTCCCCAAACTATACTGTGAGATGTTCAAACTGTCCCAGCTGCTGCAGCCCCTGCAGGACCCCCTCTCAGTTGCCATTGGCTCTTTCCAGTTTGCCTAAGAATGTGAGTTCCCAGCCTTCTCTATCCAGGCTGTCTCTATAAAGACACTTATTGCTGGGATTACTTAAGGAAACAGCATAAAGATAAAGGTCTTTCCTGGAAGATTCTGCCCTTTTCACCCTTGTCTTGTTTCCTAGTTGAGAGGCGTGTCCCATCACCAAGGTTGCATGACAAAAGACAACAAGAAAAGTCTAACAGGTTCCTTCCTGAGGCCAATCTCTGGTACCCCTGGCTTGACTGGCTCTCACAATGACTCCACACCTTCTGGTAGCTGGTATGACAGTTGTTAGAGACTGGTGGAGGCTATGTGAAAAAGGAAAGGAAGCTAGAAGAATTGTCCCGCTAGAGGTCAACCACTGCGGAAGCAAGACTGTGTGTGAGCATGGCCTGGGGGCCTTGGGGGTTTATGGGCTTGTAAAGTGGGGTTGGGATGGGAAGGTCTGGCTGTAGGTCAGTTCCCCCCACCCATCCCCAGTCTCAAAATGGTAGATACCAGCCGGTGGGTATGTGGATCGCCTGATCCAAAGCTTCCACAGGTGTTATGTGTCGACTCCGCTGTCCAAGTCTGAGAGACTGTCCCACCTGAGGCTTCTGGGTAATTAACTCTTCAACAGGCAAGTTCTACCATGGGAAACCCGTGCAGTGCTGAGGTAAAGCCAAGGCTTCTTGCAGGCTTAGGCAAGCACTCTACCAACTGAACTGTCTCTCCAGCTCTCTTTAAAAGTTTTCCAGTCGGGCTGGAGAGGTGACGCCGTGGTTAAGAGCGCTGACTGCTCTTCCTGAGGTCCTGAGTTCAATTCCCAGCAGCCACAAGGTGGCTCACAACCATCTGTAATGAGATCTGATGCCCTCTTCTGGTGTGTGTCTGAAGACAGCGACAGTGTACTCATATAAATAAAAATAAACACATCTTTAAAAAGGAAAAAGTTTTCCAGTAATCAAAAAGTTTCTGAAGGGGTAGAACTCAAAAAACCAGGGAAGGCTGTTTTAGTGACCTAATCAGACTGAATGGGGAGACCGAGCAAGGCCTGTCTGCTTGGACTTCTCAAACAACGACCTGTCTTGAGTTAGAACTCCTATTCCCAGACAAAGATTAGGATCCTCTGTGGTGAGTCTCCCAAGTAGAAGGGGTCAAAGAGGTTTGCTTTTCTTCTCCTTTAGTTCAGAGTGACAAAGGGCCGGCACTTACTATCTTTTTCTGGGTGGACATTAGTAACAGTAACAACTGCGGAGCATAGTCACTAGGTCCTGCGCTGGGAACATTTTGCATGTATAATCTATATTACTTTGTCTTTAGTAAAAACAGGTTTACGTTTCCATGTAAACTCTTTTATGTACCCTTGTCAGGGTGGGGGTGGGGGAACCCAAGCACACCCTCTTGGATCGTACTCCCAAATATCTCAGCGGGCAGTTCCAACTGTTAGCTCTCGCTCCACACCGGGGCACTACTGAGCGGACCTGCACAGTTTGGGGGCTTCTCGGGCCACGCAAGCTGCGGTGAAGCCTGAACTTCAGGCATCTAAGTATCTGCCTCTGTAACACTCAACATGACAGCCATCCTTTACCAAAGAGCAGTTGGCTGTGGGGATCTGGGTCCAGGGCCTCTGGGTGAATTCCATAAACAACCTCAGGCTCTGTTATAAAGTGGCAACTTTTAGGTTTGGAATAACAATCGGTGTGTGTGCTGGGAGGAGGGTTTGCTTACTATGAACAGAAGGCTTCAACAGCAAGAAGTTCCTGTCCTCAGCTACCAGCTCTGTATTCAAAGGGGACTTGAAAGGGGGCAAAAGGCCATCCCACTGTTACCCAGAGCTGGGCGGCCTCCTCCAGCATATTTGCATAACTAAAGGAGACCACTTAAGGAATCCTGGCTTTCACAAAGGAAACCTGTTCCTAGGTGTAGTACCGGGGAGTAGGGGACGCTTCTGGAATTCCGTTCAACACTATATTCAAAGGAAGAAATGGAGGTCTCCGAAGGATCCCACAGCTATTGTGTGACAGGGATTGACTGGAACCCTATGCTCCCAACCCCCACTCCATACCATTTCCCCCGGCATCCCTCCCCTCCGGGTTCCTTACAAACAACCTAGTGGTAAATGTGTTTGCTTCTCTGTAAAAGCCTCCAGCACGCCAAAAACTGTTAGATTCAATGAGTGACGGCATTTGGAGAATCTCAGAAGCATAGCACCATAATTATTTA
U5_15 88186104 88201152 15049 TTGGGCATTGGATTCCCCAGAGCTAGAAACATGGAGGCTAAGAGCTGAACTCTCTCTCTCTTTTTTGTAAAAGATATTTTCTTTATTTACATTTCAAATGTTATCCCTTTTCCTAGTTTCCCCTCCAAAAACCCCCATCCCCTCCCCCTGCTCCCCAACCTACTCCTGCTTCCTGGCCTAGGCATTCCCCTATACTGGGGCATCAAGCCTTGACAGGACCAAGGGCCTCTCCTCCCATTGATGACCAACTAGGCCATCCTCTGCCACATATGCAGCTAGAGGCACGAGGCTCTCCCAGTGTTTTCTTTGATTGGTGATTTAGTCCCAGGGAACTCTGGTGTTAAAGGGAGCTCTGGGGGGTACTGGTTAGCTCATATTGTTGTTCCTCCTAGGGGGCTGCAAACCCTTTCAGCTCCTTAGGACCTTTCTCTAGCTCCTTCATTGGGAAACCTGTGCTCTGTCTAATGGATGGCTGTGAGTATCTACTTCTGTATTAGTCAGGCACTGGCAGAGCCCCTCAGGAGACAGCTATATCAAGCTCCTGTCAGCAAGCTCTTGTTGGCATCTGCAATAGTGTCTGGGTTTGATGGTTGTATATGGGATGGATCCCCAGGTGGGGCAGTTTCTGGATGGCTTTTCCTTCAGTTTCTGCTCCACACTTTGTCTCTGTAACTCCTGGTCCTCTGAACTCTTAACAGCAAGTGTTCTTAACTTCTGAACCATCTTTTCAGATTTGTTTGTTCTTGATCATAACAGTCAGAATTGTGCTTTTTTTCTCTTTCTCTTTCTCTTTCTCTTTCTCTTTCTCTTTCTCTTTCTCTTTCTCTTTCTCTCTCTCTCTCTCTCTCCCCCTCTCCCCCTCTCCCCCTCTCCCCCTCTCCCCCAGGCTGGCCTCAAACTCACAGCACTCTGCTTACCTCTGCCTCTTGAGTGCTGGGGCTAGAGGTGTGGCCATCACATCCAGACAAAATTATCCTTTAGAAGTGTCTTCTCTCTTCTCCAAGACCAGACTGCACTGGTCTTCTCATTTTAACTAAGATGCTTTTCAGAGCTGAACTTCTAGATTTCGATGGAATCCAGTTCATCAGGGTGTTTTGGGTACTGTATCCAAGAAATGTTCATTTCCCTCCTGTGCCTGGCACCAAGCCTGCACGCCCTCCTTGCTCAGTCAGTGGGTGTGAATAACAGAGCAAGGGATGAAGCGGGAAGCAGGAAAGAGACATGGATGAAGAGGGATTACAGGGTCTCTGGGGAAGATCCCAGAGGGATTCCTCCTTTTTTTCTTTTTTCTTTTTTCATTTGAACCCAGGGCTTGTATATATGAGACACATGCTCTACCAATGGAGTCACATCCCCAAACCCACACTGCTTCTTAAGAGGCTCTTAAGAGACAAAAGCCTTATGATCCCACAAGTAGGTTAGAGGCAGACCAGATTCCAGAGGGAGCCCTGGGGGAAGACTGAGAAGCAAGGGAAAACGGGGTGCTCTGAACCTCTGATCTCCTGTCAGGAAGTAAAAGATGACATCTCTCTGCTTTATATAGTCTTCTACCTCTCCCCTCCGATTCATTTTCAGATTTATTTTTAACTCTGTGTATATGTATGTGTACTATAACACTAAAAGTAATATGTGTATATGTATATGTATATAATATTATGCATGTGTATGTATGTATGTGTGGGGATATGCACAGGAACGGAGGTGACTGCAACAGTCAGAAGAGGGTGTCAGGTCCCTGGAGCTGGGGTTACAAGTGGTTGTGAGCCTCCTGACATGGGCTGGGAACCAAACTCTGGCCCTCTTCCATAACCAACTATACTCTTAGCAGCTCAGCTATCTCTCCAGCACCATCCCCCCCCCCCACACACACACACAATTTTTCTTTCAAACAGGGTCTCATGTAGTACAGGCTAGCCTCACATTCGCTTTCTTGAACTAATCTTCTCCCCTCCACCAGCTAAGGGCTGGGATGGTGCCAAGATACCTCTAGCCCCTCCCCTTCCATCTCCCCCCACTCCCCCACTCCCTGCCCCCTTCCCTTCTCACCTGTTTGCAAACTTTGTCTCTTCTCTGTGAGGTCTCAGCTCATGTCTGTCCAAGGGTCTTCCTGGCTTCTCCTGTGACCTTGCAAAAAATAGTCCAGGATTCTGTATGTAGTGAGCTAGCTTTAGTCTTGTGTGTCCTGTTGTTACCTGTCTTCACCTTACAAATGGCTTCCTGTGTCTTAACCAAGCCACCAGAGAATGTTCACAGATGTTTGTTTGTTTGTTTGTTTGTAGACAGGATTTCTATGTATAGCTTTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCAAACTCAGAAATCCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGCTCCACCACGCCCGGCCTTCACAGAGACTTTTAATGCTTCCTTCTTCAGTGACCTCAAACCTGTTAGGGAGGACTGAGGGTCCACCCTGGCAAGGAAGATGGGATTTCTGATAAAACCTCCTCAGATCTTTGAAGGCTCAAAGGTCCTAGAGGGATTTTTGCTGTCCTTAAGTTCTGGGTATTTTGTTGCCACCCAATCTGCTAGAGCAGGCTAAGACGGACAGGTAAGGAAACAGTTTACAATAGCACCATCATCCTGAAATCCTGAGTGGGGGGTTATCAAAGCACAGATGACAAATTCTTGGCTCCTGAAACCAAACCAGGAGAGAGAGGCTCAGCATGGGGCATGTTCCAAGTTCCTCATACAGCATATCCATTGATTACAACATGTGGCTTAAAGCAGGATGCCTCCTTGAGTCTCTCTCTCTCTCTCCCCCACCCCCATACACAACACTGGTTATAATCTTATCAGAGCAATTGATTCTCTGAATCAGATGAATCAGATTTTATATGGTTGAAATCAATGAGTTAACCCTGTGGGTTACTAGGCTGTACACCCTTTTGGGTGTGACAACTGAAACTGTAAGGCTTCAGTTCTTCCAGGCTGTTGGGCAGGGCAGGGTTCCTGGGTTCTTCTGGGTATCGGTTATCGGCAGAAGTCATCCTGGGTCCAGTCTACACATGTGTACCTACCTTCAGGAAAGAGAGAGGCCACGGTCCTCACCTGCTCTGGTCTCTGTCTTTCTACTCTTTTGTTTGTTTGTTTGTTTGGGTTTTGTTTGTTTGTTTGTTTGTTTGTTTGAGACAGGGTTTTTCTGTGCAGTCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCACCTGACTCTGCCTCCCAAGTGCTAGGATTAAAGGCGTGCACCACCACGACGGGCCGGCTGATCTTTTTAATTTTTAAAAAATGTTACGCATATGAGGGTTTGCTTGCATATATGTTGTGCACCATGTACATATCTGGTACTCATAGAAGCCAGAAGAGGCCGTTGATCCTCTGGAACCTGAGTTACAGACTGTTGTGAGCCACTATGTGGGTGCTGAGAACTAAACCCAGGTCTTCTTCAAGAACATCCAGTGCTCTTAACTGCTGAGCCATCTCTCCAGTCCCAAGGTTCATAATTTTCATGTAGGCCTTTTGCCAGTAGGCTAACGTAGGGTCAAGTTCTGCGTCCTAGGTGAACATCTTTGCTTGTGGCCACAGGGCTTCTGGTACAGGAGTCAAGCCCTGCTCTACCTGTTCCTCTCCCTTTTGTGTGTGCTGTTTCATTCTGTCCCCTGCAGCCAAGAGCCAGGAATATAAGAGATCTTCTTCCAGGATATTGAGTTGCACAATCAGTGAGTAAAGGTACTTGCTGCCAAGCCTAAGACATGAGTAAGTCCCAAGAATTCACGTGGTAAAAGGAGAGAATGAATTCTTACTTGTTGTTCTCTGACCCCATAGCATTCTATGGTACATGTCCACATACACACACACACACACACACACACACACACACACACACACACACACTTATAAATGTAACAAAAAAAGTTTTGTTTTGTTTGTCTTTTAAGACAGGTTTATTTACGTAGCTCTGGCTGTCCTGGAACTCACTCTATAGACCAGGCTAGCCTCAAACTCACAGAGATCCACCTCCTTCCTCCTGAGTACTAGGATTAAAGGCATGCACCACTACCACCTAGAACAAAATGTTGTTTTAAATTATACTTATCAGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCTCTGACTTCTCTCCAGAGGTCCTGAGTTCAAATCTCACAACCATCTATAACGGGATCCGATGCCCTCTTCTGGTGTGTCTGAAGAGAGCTACAATGTATGTATGTATATGTATGTGTGTATATATATATATATATATAAAAGTCTTTTAAGAAAAATTGTACTTATCAGAGAGCAGTCCACTTTAAACACCCAAACACCCAAGGCTGAGCCCTGGCTGTCCATCACGTTAAGCATGCACACTCTGGGCAGGAAAGGCAGAAATGCCAGTCAAGACCCCTGCAACTCCAGGCAGACCCAGAAGTCCAGAGGCCAGCGCTGAGACTACAGGCTCTGTTAGGTATTAGGTACAAGGTACAAAGCCTGAGGTTTGGAGGCATTGTCACAAGGTACCATCTGCGGCCTCTTCTCCAGGTGTGTGGAGGTTGCTCTTTCACCACAATTCTATCAAATGAGATCACTGAGGTCTTTCCAGACTATTGGCTTGGGATACAGTTATATTCGTCGCCTTGCTCTAAGTCTCATTGTGTTTCTCTGTAAGATGGAAATCAGAATAATCTTTGCCCGGCCTGCCTAGTTAAGGGTTTTATGGGAAGGAGATAGGAGGCTAGAATATGGAGAGGATTCTGGGGTGTGTGTGTTGTGTGTGGTGGGAGAGACAGACAGACAGACAGACAGACCAAGAGACACCCAGCATAAGCACACTGTAGAGCCTAAAAGGCATGCATCTCTGGGTACTTCAACTCTCCAGCCCACCCCTCATCTCTCTGGCTAGATATTTGGCTGGCTGTCATGGGAACAAGGCTGTCCAGAATGCACCTGGTCCGGGCTCTGGTCTGAAAAGGCAGCCATTCAAGGTTGGCTTGGCCAGGAGCCTTGTGATGAGAGACAGGGGGCCCTGTGCTCTGGAGGGAAGGAGGCCCATGCGGCCTCAGTGTGGAGCTGAGCTGTCAGCCTGGGAGGGTGGTAGGCTGGTTAGGAGGGGGAGGGGTCTCAAAAGGAAGACTGAGCTAGTGTTTAAGGCCTAGGACTGAAAGGCGGCTGTGCGCCCCAGGCTAGCTGCCTGGCAGGCAGGCATGTGACTCATTGGTGGCCAGAGGAGGGTGCAGCCTGGGCTCAGCTAGCCATGACAGGGGGCTCACTGCCCACCTGCATGTGAATGTATGTCGGTATAAGTTGGAGCATGTATGTTAGTGCTGAGGCTACCTATGTGCCTGGGCTTTTAGGACCCCTGGCAGTAGCCACATCCTGTCCTCTTCTGACTGTGACCATGGCAAGTCTGCCCTCCTCCCACTCAGGGTCTTTTGAAGTGGCCGACACCTGCCTTCATCCCCGCCCTCCATCCTATGCTTCCCTGCTGGGTGCTGCCCAGCAGAAAGGGCTGTCCTCAGTCTCTAATTGTACCCTCTGTGGTAGGACAAAGGGGGGAGGGACAGACAGCTTGTTAAAGTGATGCTAGCCATTCTAAGCCCATTTGTAAAGGCCATCAGGAATTCATACCATTTCGAAGGAAGGCAGGGTACTGGGGAACCACAGGGAGGCAGGCTTAAAGGTTTGTGGGTAGAACCAGGGATCCTGGAGCAGGCACAAAGTTGAGGACCTGGGTGTGATAGAAGAGGCTACACAGGACCTATTGGGTCCCAAGCCTATTCCCAAGGGCCTGCCAGGCAAGGTTTCATTGTAACAAAACAGCTGGTCACAGACAAGTGTATATTTATGGGTAAGCAAGTATCTCAGAACTTTGTGTGTATGTGTGTGTGCTCTCGTGTGTGCCTACCCATGAGTAGTTTTCTACATGTCTCTGAATACTTGTGTATTCATGTGTGTAGCTTGATCTTACTGTACATGTGAGTGTCTGGCTACATGTGTTGACAGTCTGTGTCATATGCACATTTATGTGGGTTTTTTTTTTTTAAGATTTATTTATTTATTATGTGTAAGTACACTGTAGCTGTCTTCAGATACTCCAGAAGAGGGCATCAGATTTTGTTACGGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTTGGAACCTTCGGAAGAGCAGTGGGCGCTTTTAACCATTGAGCCATCTCACCAGCCCCCTATGTGGTTTTATATATGTCAGTGTGTGATGGTGCATTTGTGTATCTGCCTGTGTGAGAGAGAATGTTTGTGTGTATGTGCGCCCTACCAAGAGGAGCTGGGGTTCAGCTCCAAGACTTAGGCCATACCAGGAACCTACCACCCATCAGCCCTCCACTCTTTCCTGGCCCTCCCTGTCAGGTTTTTTCTGCTTTGCTGACCCTGGAGCCTTTAAGCTCTGTCCTCTGAGTGGCAGACCAGCTCCACTTCCACTTAACTGCCACCCCATACCCACAAAGCCATGGATGGCCTTGGCTGCCCACACACTAGCCGGCTTGTGATGGAGCACGGAAAGGGACCAGAAAGCTGCTCAACCCCAGAGCTACTATGTCCATCTTCTCCTGGCCCAGGGTTCCTCCCCATCCCAGAAAGAGGTGTCTAGACAGCATGAAGCAGCTGTCCACACTGCACTGCCCTCCTTCCTCCTGGGCTCTGCATCTGAGAAGTTTGTCTTACAGAATAGCCAGTGAGTCCTGGAGGCTTAGCCTAAGTAACACACATGGCTGGGACATCTCCAAAGTATCTGAGTGAACCTCTGACTGAGCAGAGTTTTTAAGATTGGCAGAATCAGAGCCGGGTGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGTAGATGAATTTCTGAGTTCGAGGCCATCCTGGTCTACAGAGTGAGTTCCAGGATAGCCAAGGCTACACAGAGAAACCCTGTCTCAAAAAACAAAAAACAAACAAACAAACAAACAAAAAGATTGGCAGAATCACGGGGCAGCGGTTCCAGCTCTTGGGAGGCAGAGAGAGGCAGAGAGAGGCAAGGGGAATTCTGAGTTCTAAGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGAAAAACCCTGTCCTCAAAAATCTACGCCCTGGGAGGGGGAGGCAGTCTCCTTTGAAGGCTCTACCCACCTCAGCTGTTCCTAGGGGCTGCCAATTTCAGGATCTCGTGCCATGCATAGAGTGGAAATTATGACCCAGGACTGGCCAGGGATGCCACCCTAGCCCTGGGATGTGCTCACTGCCTAGGGTGGGACACATGACACCGAGGGAAACCAGAATTCCTCCCTGGGATTTTTTATGAGGAAGTGGAAGTGGGGTGAAGTGATAAGTGCCTAAGGGCATCTTCTTGTAAAAGGACAGGATGGCCATACAGTCACAGAGTGTTAGTGGTATTTCTATTATATAAGTTAACAAGTTTCTTTTGCTTCAGCTACTTAAGAGTTGGGACTTACTATCACTTAAAGCCACTGCTGGCCAGTGGTGGCACACACCTTTAATCCCAGCACTTGGGAGGCAAAGGCAGGCAGATTTCTGAGTTCAAGGCCAGCCTGATCTATAGAGTGAGGACTGAATTCCAGGGCAGCCAGGGTTACACAGAGAAACCCTGTCTTGAAAAACCAATAATAATAATAATAATAATAATAATAATAATAAAGTCTGAAGAATCCAGGATTATTTCTTGCCTGAGCCACATGCTTGGTCTTCAGGATGCCAGAGCTACTTTGCCTGGCTCGCAACATCCTTCATGGTCCCTTCTTGGCCCTCTCAGATCCCACCTAGATGTCTTACTGCCAGCATCCACTCTCAAACTCCACTGTCTCTACCACCACCTACTTCCTCAGCTCCCTGGCCCCAGTCTCTTCCACAGCTACTTCCCTGTCTGTCTGCTTCTCCCCAGCCTCACAGGAAGGCTTGTCTCTCTTCAGGTCCTTATTCAAAGCTGACCAGCGGCCTCTGGAGTGCACCAGGAAGATGGCTGGAAAACCAGAGCACTGCTCTCTTCCTCTAAGGCTTTCCAAGGAGCTCTCCTCGGTCTGGCCTTGGGACCAGCACTGGGAAACCGTCTGTCACTCAGTTCTGTCACCTCCTTGATTGCTGAGGCTCAGCTCCATGAGGACAATCAAAGGTTACCTTGGGTTCCCTAGAGCTGGCACAAGTTGCCTCACTTCCTCCATTCCTGACTCCTTCCCCTGTGATCTGCATTTCTTTGGAGTCTAGTCTTCCTTTGGGTCCTGCGCACACACATACATGCTCTGTCAGAAACATAGAGGACCACGTTTTATTGAATAACAAATTCTCAGAGTGAAGACGTTTCACTGAGGGCAATTCATTAGCTTGTTCCACTCTCCTCCCTAGCCTTGGCTGAGGAAAACACAGATTCTTTCATTCCAACCTTAATTACTAATATTAACTCAGGTCATACATTACAGATCTCCTAGCAAATCTATCTATGTATACGGGTATAGTTGTAAGCTACAGAATGTACACTAAAGTTTAAGATGTCTCAGCAGGCCTGGGTCCTCCCTTCAAGTCAGAGGCCAAGAAGGAGGCAAGGAGTTGGACTTCAAAGCATGGGGCGTCCTCCATTTAAATCTAGTTGAGACTCTTGGAAGATAAAGCTCTGAAGGAGTTTCAAAGCGGCCTAAGAGACAGAAGCTGATAGGAACACAAAGAGAGTTGGGCTGGGGGTAGAGAGGTGAGATAGTAACATACCAGGCTGGAGAGGCGCCGGAGCTACCACTGACAGTTACCACTACAGAGCCTGGCTGCCGGCAGAGCAGAGTTGAGCCTCAACGTTGAGTCTTGCATCAGGGGTGAGGATCTAGACCAGTCCAGAAGGATGGACAGATGGATGGCAGGTCCAGATGGACAGACAGATGGAAAGATAGCAGTTCAAGGAGGGTGTGTCAACATCAGGGAGTCAGCCAAACTGAAGCCCCTGAACAGTCAGAAATTCTGTCAGTCCACTGACATAAAACCCAGGTGGTCAGGTGACAGACAGGTCAGTGGGACTAGAGCCCATGGCAGGGTAGGGGGCCTGTCCTTTCGTGGGGTCCAGGTGGCAATGTTCCACCGTTTCCTCTGTCTGGTGTGTGCCTTAAGTCCTCAGGCCTCATTTTCCTGAGAGGACTGTGCTCATACGCTCACATGGTCCCAATTTACTTTGATAAATATAAAGGGCGACACCTTTCCTACTTATAGGAATAAGTTATTTATCCGCCTGCATCTCATGTACGACAGCATTTGTACTCAAAATGAAGCCAAAAGCCGCTTGTAAAATAGTGCCTCTCAGGGCAAGGGGCAGCCTGGAAAATACTGCTATATACAATATGGAAGGACTTAGGACAGAGACTGCCTGCTCTTAACACGTTAGCTAAAGGAAATTCCAAGAATTCAACTAGACTCTTATGTGTAAGATACCCCAGGCTCGGAGGAGTCTCCCTGCTCTCCCCTGCTCTTCCATCTCCTCCCATCTCCTCCTTGCTCCTTCTTGGGCCCCTGACTTACAGAGAGGACCTCTGTCTGTCTGTAAGATGGTTCAGCTGTTATATCTCACCTGTCAGGTTTATGTCATATACAGACACACACACATACCACATACATACATACATACACACACATACATACACACACACATATATACATACACACATACATACACACACATACATACACACATATATACATAAACACACATACATACACACACTAAATATATAAGTGTACATTTAAAATTGAAATAATAAAGGGGAGCCAGCCTCCAAGGAAATACGTCCCCTCTGTTCATCCCTTAGCATTCCCCTGCTCCAGATCCTGCCTCCCGTGCCTCCATGTGGGGGAGACCAGACCATTTACCTGTGGGGACTCTGCCAGTGCTGACCTGAATAGCATCCCTGGGTTCTCCCTGACCCCTTCAGCACCACCCTGTACTGTGTCAGGTTCTCAGTATTGGACTCCTATGAGGACCAGAGGTTCCACTCTCACACTTTTGTTTGTTTGTTTGTTTGTTTTTGCTAGACAGGGTTTCTCTGTGTAGGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGTATGGCCTCAAACTCAGAAATCTAGCTGCCTCTGCCTCCCAAGTGCTGGAATTAAAGGCTCAGTATGTGGGCACACTATCACTGTCTTCAGACATACCAGAAGAGGGTATCAGATGCCTGTTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACCCGGGTCCTCTGGAAGAGCAGTCACTGCACTTAACCACTGAGCTATCTCTCCAGCCCTCATTCTTAACACTTTCTCATCAGATATCATCTCAGACCCAGACTTACCCACTGCAAACTTCTAGAACAAAATGCAAAGCAGGCATCCTACCACGGTTCTGAGCTTGGAGAAACTCCTTTCAAGGTCTGCATAGGAAAACATGTCAGGGCAATTCTCAAGATCACCCTGACCAACAGCTAGCCCTGAAGTCACAGAGGACCCATCATAGAAAGTTCCAGACCAGGGTAGTGCTCCACAGAGCTGGTCTGGGATGGACAGAGGAGCCATGCAATTTTTTTTCTTGTCAGGCAACTCTTTTCCTACCAGCCATTGACCTCTGACTGCCCCAGCGCTGCCTCACTGGCCTCCAGCTGTGAGTGTAGACAGCTGAATTTGAAGCCCCAGCTGTGGCTACCCCTGGAATGTAACACTCACTAAAACTAATTCACATGCCCTCAACTATTAAAGTGACTGCAGCTGACAGGGCTTGTTAGTCCTTCGGCCACTAGCTCCAAGTCTCTGCCCATGGCAGACTCTCTCCAAGGTTCCCATGGTTCCTTACTTTCTACTTCCCTGTTCTTTCTGACCCAGGGCAGCCTCTACATTGTCTCGCAACAATATCTACCCAGTATGCCCTCTCATGGGTCAGTTCTCCCTCCATCTTAAGTCTCTGCAGACCTAATGGGGAGGGAGAAGTCACAGGGAGAGGGAAATGGGAGCTGGGGAGAGCAAGTTATCCAAAGAAGGAGTGGGAAAGGAGAAAAGGGTGTTTCTTGATGTACTTCTATATGCTAGGTCACTGAGCACCCCTTCTGGGGCAGCAAGAGAACGTTCAGAAAATGACTTGCTGGTTTAAGGACTCTATTCAATCCCTTCTTCCACCCACACCAATGCTTCCTGGAACTGCGGTCTCCTCCCACTGAGCTCTCACCACAGCATGGGGGGCAGTGGCAGAGTCCACTGGCAGACTGGCCTTGCTCAGCCACTCCAGTTGTTCTGAGACAGTCCCTAGCCGGCTGCTATGCCCTCGTGGCTGGACAGGTGGAGGGAGTCAGACTAAGGGAGCCCAACGGTGGGGGTGGGGAGTCACTGTGGTTCTGAGTAGCAATGGGGACCTGTGGCTCAGTGAAAGAAGAATAGGGTGACATTGGAAAGTGACCAGAGCCTGGGGAACACACACGCACACACACACGTGCAAGGACTCATGTACTTCATTGTTACACATTTCCCCATTCTGAATTCACAGACCAAAAGATCTTTGACAAACTGACCAGTTCAACTGAAAAACCCAAGGTGACCTTGGGCGGTTCCCCACTTCCCCTGCTTCCCTGTTCCCTTGGGATCCCCTGGGATAGGTCAAATATTAGTGATCATAATATCAAAAGCACCCACCTGTCATAAGGCTCAGCGTGTAGGGGACACTGGTGTGTCTGCTTCCTCTGTCATCTCACATAACTCTGCATTGGCAGGAAATTGAGGTCTCAAGAGGATAAGCCAGCTGCCCCCCAGCAGCAGCCAGGAAGTGGGGAAAACTGGCCCAACCCCCAACCCTCTGGCACTCAGCCCTTACCAGAAACACCTGTGTCCACCTTCACCTACACCTGGAGAAATGCCACACTGGGATGAAGGGTGAATCAGAGGCCAAGAGGGCTGGTCTCCCCCAAGGCACCTTGTGGAGAGCTGAGACAGAGCACCTGCTCTTCCCCAAAGTCACACTTACAGGGCAAAAAGAGAGACGCCCAAAGCCTGGGTGTGCCAGCTGTGTTGGCACATTTATAACCCCCATCGTTCAGAAGGCTAAGGCAAAGTGATCTGGAGTTCAAGGCCAGCCTGAACTGCATGCCAAGACCCTGATTCAAAACAAACCACAATATCCTGGGTGAAATAGCAGAGACAGGGATAGGTAGCCAGTACCAAGCTAAGACAACCAGGACTGAAGACTGAGGACCGGTGTGACCTGGGACTAGTGGGACTGCAGTGACTCCTAGGGGCAGGCAGCCAGGCCTGTGAGACTTACACTTCAGGCCAGGGCTTCTTGAGGACTTAAAAGGGGCATTGTCCCAGGATTCTCCTCTGAATTTTTCATCTGAAGGCCCTTCATCATTTCCGACTGGAGCTTACAAATTCCAGAAAATTCCACTATTTGTTTAAGCACACACCCGAGCCTAAGGATCGCTAAGGGAAGGACAAAAGTAATCTTCACCCTTAGGACTCCCTGGGACTTTCTGTCTCCCTGTATTCCCTGGGAGAGGAACAGCATTGCAGAAAGGTATTGAGTTCCCCAGTCCTACTATCTGATAGAAATGCCTGAGCCGGGACTCAGGGACCACCTCCTGAGCCAGGCAGACCTCCTGGGTTTGAATAGTAAGGTTGTGACTTCATCTAAGGGTGTGTGTCCCTGACTCTAACTCCTGGTAAGATAGGAACAAACCATAGCACACGTCTCCAGAACCAAGTGAGCCACTGATGCCGGGGACCTAGAACCAGGCTTGACAGATAGGAAGTGCTGGAATGTAAAAAGTCCTGTGGGTATTTCTGAAGCGACCCCAGACCCCCAGACATCTGGATGACAGAAGGACCAGTAAGCAGCCTTATCTACCAGAAATGTTCCCAAGTCACCCAGAGGAGGCATCAAGGTGTCCCCTCCCTCCCTGTACTGTGATTGTGGTGTGTAGCCCACTCCCAAGAGAAAATGGTGCTCACACCGCAGAATAATAAAAACCTGGAGACAAAAAAAAAAAAAAAGTGGGGGTGCGGTTAAGTGAAGGATTTGTTGGCAATACAAGAAGGAGGTTTTTGTTTGTTTTGAGACAAGAGTCTCATTAGGGAGACCTTGCTGGCCGGGAACTCACCATGTAGACCAGGCTGGCCTTAAACTCGCAGAAATCCACCAACTGGGATTAAAAGCACATGCGAACACGCTAGCAAAAGAATGGACTTTTTGCAGAGGATAATAAGCCTTCTCACTGGGGGGTGGTAATGCAGAAAGAGTGGGAAACCCAGAGCTGGATTGACCTGAGCTGGCTGGGGGTCTAACACAGGCAACCCAGCATCATCTTCTCTTGAGATCGCCGCCCAGGCCTAAGCGAGCCCGGGAAGAAGAAAAGTAGACAGGGCAGAGATAAGGTAACTTTAGGGAAACAGCCTTTATCCACCTCCCTCCACTCTACACACCCAGGGCTACTCCAGAAGGAAGCAACCAGGCCGCTAGGCGCCGGGTGGGCGTGGAGGTGGGCGGTGTCCGACGTCCCCACGTGTCCCGAAGGAGGCGAGGCGGGCGACCCCGTGAAGGCCCAGCAGCGGGGAAGTCGCCGCCTCAGGATGTTGGCGATCTTCAAGGTCTCTGAGATTCTGGCTCTTCCCTTCGCGTTCCCGTGGGGCGTGCCTCTGAGGTGGCCAGGGCTGAACGGAGTTGCTGTCCCCACAGTGAGCCGGGGCACAAAGGGTTAACCTACCTGGGCCACCGCCCCTGAGGCCCCTCCTCCAGCCGCAGGGAAAAAAAGAAAGTCTCAGCGGGGGTGCGGCTCGGCCTCGTCCCGCTGTCCCCAGCCGCGGCGCTCAGAGCTGTGATCCGTGCGCCCCGCTCAGCTGCCCTGGACCGGGCCGCCATGTCTCTGTGGTGAGTCGGGGTCCCGGTGGGGCGCAGAGTACCTCCGAGCTTCACCTCCTCCCGGGGCCGCAGACTGCAAGGGATGCACGCTAGGTGTCCTGAGGCCGACCCCAGTTTCCCCGGGCAGCGAGGGTGGCGGGAGATGCGGAGCAGGGCGCGCCGCCGGAGCGGCAGATGGGATGTGGGAGATGCGAGGCGCACGCTTGGCTCTGCGGCCACGACTCCGGCAGCCTGTATGCAACGTCTCTCTAGCCTTGGGGTTTCACTAGCAGACACACACACACCCGACCCCTCCGGTCCCTCTTCCCTCCTGTGGCCTCTTCTCCGGACCGCCTCGACCCTTCTCCTTCCCCGGCTTTGCTCTTACTAATGGAGTGGATTGGTATGTGCGAGCCAGGGCCCTGAGGCTGGTTCTTACCCGGACTCCGGAGCTACGACGTGGAGCTTCTTTAGAACCCTGAGGACTGGAGTACTGGGTTCAGAATTCTCTTACTTCCATTCCCAGCAGCGCGCCTCCAGTCATTTCTGTCTTTCTCCATCCAACCTGGTAACCAGGAAGTCATGGGGCTCTTGAGGAAAGCTCGATGAATGTGGGTAGGAGGACTTAACATTCACTCACCCTGCGCCTCCGCAGGCTTAAAACCTGGCGTTATCTACTACGTGCTTCGTGGGCCCCTGGCTCTTGTACCTCCCAGACTTGGAGGTGAGAACCATCTCTCTGCTGGCCCAGTCCTGGAAAGTCATGAGTCTCCCAGTGTGAGCTAAGGAGGGAAGGTTCTTGGGGAAGGGGGCACAGGCAGGATCATTCCTGAGAAGAGCTAGGATTTCTGCCTCTGAAAAGGGTGTGCTGGGCTTACTTGACCAGGTAGGGGCCCCGGAAAGTGGGTTTTCGTCCCCAGGACTGAAGGGCTAGGCACCTCATTGCTTTGTGTAGAATATAAAGGTAAGGATAAGGAGTCGTGATGGGATCTGTCCACTTCCCTTCGAGACTTCCCAATTAGAGCAAGAAAGGCTCGCTTCTGTTGAGTCAGT
D3_15 88204118 88219168 15051 ATTGAAGTAGACTTGGCTGGAAGGAGAGCCCAACACATGCAGGACTTTTGCCAAAGCTGCTGCTTCCGGAGAAAGGAGAGCGGTTACTGCCAGGCCAGGATCTTCTGAGATGGTAGTGAGCAGGCCAGCTGTGTCCAGCTCAGAGCGCTCCACCATGGGCCCAGCAGCTCCGATCCAAGTCCTAGGGTTGGAAAGGACAGGGCTGCCACCATTTCTCCCTAGCACAGGTCTTCCCACACCTCTCTTTGTTAGCTGGCTATATTATGCGTAGAGCTCAGTGCAAAATGTATGCCTAGTTCCCAGAAGTTAAAAACTTTAAGATTGCAACAGTGCAGAGCCCTTTGGAGGAGCCAGCCCCCAAAACAGCAGCAGGAGAGCTAGAAGGGCCAGACAGGCTCAGGCTGTGAAGCTGCATCTAAGGACCAGGGAGAGACATGATTTAATCAAAGGTCAAGGGAGAGAGATGGATGCTTGGTTGTGCAGCTAGGTGGCAGGCAGGCCTGCATCTCAAGCCTCGGAGGTTTAGGGAAGTGTCTGGAGCTGACCCTAAGAATGGGGCAACAGGTCAGTAGTTCTGAAGCGCCTACTTTGGCTTCCTTTGTCTTTGTCTTGGGTCCTGTGGACTGGGAACTCAGTGAAATTCGCCCCTGTACTGTCGGCCTTGATAACTAGTGATATTATCAAGACTCAGGCCAGTCCCTTCTTTCTGAGCCTTGCCTACTGAGCCAGAGTGTGTGAGGTTTTTGCTTCATTTGCCCAAGGCTGGGCTGGCAGGCCACAGTAACTCAGCAGAAGGCCAAGGAGCCCCTCAGGGGCATGCTGTCACACTTTCCATCCCCCTGCACCAGCCCTGACTGAGCCTTGTACCTGCTGCTCAAAGGACTAGGGGTATCCCCCCCCCCCCAGGTAGACTGGAATGTGGGGAGGAGCCACTATGGGGTGGGGGTAGGGAGGGGAGACAAAGGGGAGGTTCCCTGCTGAGTTTGTTAACAGATCTCAGCTCTTTGGTCTGGAAGTGGTAGAAACATACCAGACCCAGAGATACTCAGGCAAGGCTTCTCTGTTAAGACATGGCTCCAACCAAGAGCAGGTTTAGGCCAGTGGTTCCTAGAACAAAGGAAAGGGGTTACTCTTACAATTAAAAAAAAAAAAAAAAAAAAAAAACTCCAACCATCCATCCCATCCAGGTTATCTGAAGGGATTCAGAACCAAAATGGGTATTGTCTAGTCTTGACTGGTTTGTGTCCCCGTCCTCCCTGTAAGAGTAACAGATTGTAGAAAGCTCTGGTCCTGACTGGTCAGGGGCTAAGCAGTCTCATGCAGTTCGCTAAGCAGCTTTGACTCTCAGGGTTGGGTATATTCTCTGGAGAGTGCCTTTATCCTACATCTGAGAGAATGAATGATTGCGTTTCTCCGCTCAAGGCTATGTGCGGGCCCTGGGTCACTGTTTGAATCTTCGGATAGAGGACCAACCAAACAGGCAGGCTCCTCCCTAAGGTCAGATGGGCCTGTCTCTTTTAGGTGGCCCTGTCTGTTTCAAGTTAGGGTGCTCTCTGCAGGCTCTAAACTATCAGAACACACCAGGGTCCACATCCTGCTAGTCCAAGCAGGACAGGACTCTCTTCCCCAAACTATACTGTGAGATGTTCAAACTGTCCCAGCTGCTGCAGCCCCTGCAGGACCCCCTCTCAGTTGCCATTGGCTCTTTCCAGTTTGCCTAAGAATGTGAGTTCCCAGCCTTCTCTATCCAGGCTGTCTCTATAAAGACACTTATTGCTGGGATTACTTAAGGAAACAGCATAAAGATAAAGGTCTTTCCTGGAAGATTCTGCCCTTTTCACCCTTGTCTTGTTTCCTAGTTGAGAGGCGTGTCCCATCACCAAGGTTGCATGACAAAAGACAACAAGAAAAGTCTAACAGGTTCCTTCCTGAGGCCAATCTCTGGTACCCCTGGCTTGACTGGCTCTCACAATGACTCCACACCTTCTGGTAGCTGGTATGACAGTTGTTAGAGACTGGTGGAGGCTATGTGAAAAAGGAAAGGAAGCTAGAAGAATTGTCCCGCTAGAGGTCAACCACTGCGGAAGCAAGACTGTGTGTGAGCATGGCCTGGGGGCCTTGGGGGTTTATGGGCTTGTAAAGTGGGGTTGGGATGGGAAGGTCTGGCTGTAGGTCAGTTCCCCCCACCCATCCCCAGTCTCAAAATGGTAGATACCAGCCGGTGGGTATGTGGATCGCCTGATCCAAAGCTTCCACAGGTGTTATGTGTCGACTCCGCTGTCCAAGTCTGAGAGACTGTCCCACCTGAGGCTTCTGGGTAATTAACTCTTCAACAGGCAAGTTCTACCATGGGAAACCCGTGCAGTGCTGAGGTAAAGCCAAGGCTTCTTGCAGGCTTAGGCAAGCACTCTACCAACTGAACTGTCTCTCCAGCTCTCTTTAAAAGTTTTCCAGTCGGGCTGGAGAGGTGACGCCGTGGTTAAGAGCGCTGACTGCTCTTCCTGAGGTCCTGAGTTCAATTCCCAGCAGCCACAAGGTGGCTCACAACCATCTGTAATGAGATCTGATGCCCTCTTCTGGTGTGTGTCTGAAGACAGCGACAGTGTACTCATATAAATAAAAATAAACACATCTTTAAAAAGGAAAAAGTTTTCCAGTAATCAAAAAGTTTCTGAAGGGGTAGAACTCAAAAAACCAGGGAAGGCTGTTTTAGTGACCTAATCAGACTGAATGGGGAGACCGAGCAAGGCCTGTCTGCTTGGACTTCTCAAACAACGACCTGTCTTGAGTTAGAACTCCTATTCCCAGACAAAGATTAGGATCCTCTGTGGTGAGTCTCCCAAGTAGAAGGGGTCAAAGAGGTTTGCTTTTCTTCTCCTTTAGTTCAGAGTGACAAAGGGCCGGCACTTACTATCTTTTTCTGGGTGGACATTAGTAACAGTAACAACTGCGGAGCATAGTCACTAGGTCCTGCGCTGGGAACATTTTGCATGTATAATCTATATTACTTTGTCTTTAGTAAAAACAGGTTTACGTTTCCATGTAAACTCTTTTATGTACCCTTGTCAGGGTGGGGGTGGGGGAACCCAAGCACACCCTCTTGGATCGTACTCCCAAATATCTCAGCGGGCAGTTCCAACTGTTAGCTCTCGCTCCACACCGGGGCACTACTGAGCGGACCTGCACAGTTTGGGGGCTTCTCGGGCCACGCAAGCTGCGGTGAAGCCTGAACTTCAGGCATCTAAGTATCTGCCTCTGTAACACTCAACATGACAGCCATCCTTTACCAAAGAGCAGTTGGCTGTGGGGATCTGGGTCCAGGGCCTCTGGGTGAATTCCATAAACAACCTCAGGCTCTGTTATAAAGTGGCAACTTTTAGGTTTGGAATAACAATCGGTGTGTGTGCTGGGAGGAGGGTTTGCTTACTATGAACAGAAGGCTTCAACAGCAAGAAGTTCCTGTCCTCAGCTACCAGCTCTGTATTCAAAGGGGACTTGAAAGGGGGCAAAAGGCCATCCCACTGTTACCCAGAGCTGGGCGGCCTCCTCCAGCATATTTGCATAACTAAAGGAGACCACTTAAGGAATCCTGGCTTTCACAAAGGAAACCTGTTCCTAGGTGTAGTACCGGGGAGTAGGGGACGCTTCTGGAATTCCGTTCAACACTATATTCAAAGGAAGAAATGGAGGTCTCCGAAGGATCCCACAGCTATTGTGTGACAGGGATTGACTGGAACCCTATGCTCCCAACCCCCACTCCATACCATTTCCCCCGGCATCCCTCCCCTCCGGGTTCCTTACAAACAACCTAGTGGTAAATGTGTTTGCTTCTCTGTAAAAGCCTCCAGCACGCCAAAAACTGTTAGATTCAATGAGTGACGGCATTTGGAGAATCTCAGAAGCATAGCACCATAATTATTTAAATATTCTGATGCAAGGGTTTTCTGTTCCCACAAAGCCCTGGCACTTACTGAAATGGGAGGGGTCGGAGATCAGAGGACAGCTTGCAAAAATAAATTCTTGCCTTTCAGTAAGGTGGGACCTGGGACTAGAACTCATGTGTCAGGTTAGGTAGGAAGTGCCTTCACTTGCTCAGCCATCGGGCGGCCCCCCTTTCCCACCACTTTCTAAGCAGATGACTGCACACTGACCACTCTGGGGGAACACTGAGGTTGACTTCTCTCCCTTCGGTAGACAAACCAGTTTTCCTCCGTTTGCAATTCAATTCTACAGCTCTGTGGGGAAAAAAAAAATGCGGGCTGTTAAAAGGCTACTTTAGCTGCCGGACTATTCTGCCTCGAACCAGTAACTGTTTTCTTATTTAAACAAACACCTAAACTCGGGAGTGGGTGGAATGCAACTTTTGCATAAATTTCGGATACAAAACTGGAACTGTAGGAAGCTAGGAGATGGTTAATTCTAAATCAGTAGGTAGTTAGGGAGAGAGAGCTCAGACTGACCTCTGAGTGCTTCCACTATAGATGGAGCTGGGCCCTTGCACAAAAGAAGCCCCTCCTTTCTGTAGCTTTTTTTAGTTTTTAATTTATTTTTATTTTATTTATTATTATTATTTTATATATATATATATATATTTATTTATTTATTTATTTTGGTTTTTCGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGCATGTGCCACCACGCCCAGCTTTTTCTTTTTTTTCTTTTCTTTTTATTTTATTTTATTTTATTTTATTTTTTCACTCCTGCAGGCCTCTGAACTAACTACCAACCAGCAGGATAACTCCCTGCCCCATTCCCTGGCCAGCCACAGATAACCCTCAGGGTGGCCTTTGCCAGCTTTTGAAAGTCCCCACCCCTGTCTTAATCAAGTCCTTGCAGACCTTTAAACCGACCCAATGGGCTAAATGTATCATGCCCAGTCCAATTTAAAGGGAACCCAGCCCTGCACCACACCCTATTAGAAAATATATAAGCCAAGGTGTGGTAGGCACTCATTTAGTCCCTGCATTTGGAGGGTAGAAGCAGATGTATCTTTGTGCTCCCGAAAAAACCAGTCTGGTCTAAGTAGGGAGCTCCAGACCAGGCAGGGCTACATAGACTTTGTGTCAAGAAAAATAAACAAACAAACAAAAACCCAACACACACACAGAGAAACAGAGAAACTAACTACCTCAGGACAGAGAAAGCTACCCTTTTTCTCTGATACCTCCCGCCCCCCTCCCCGCCCCCCCACCCAGTCGCCCTTGTTAGGCACGGGATTTCGCCTCTGCAATAAAATTTGTGAAGATGGGTGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACCACTCCACAAAGTGAATTCCAGGACAGCCAGGGCTATACAGAGAAAACCTGTCTTCAAAAACAAAAACAAACAAACAAAAATAATAATAAATTGTGAAGATGGACTGCAACTTTCTTGTCCAGTAATTCTTTACCAGATAGAAAAAAGTACTGGAGAAAGGAGAACTAACAGGCAACATAAACTTGGAAGTACATTTTACATTCTCTATAGGCCCTTCAAGGTAAGCCAAGTACTGTATATATCCAGTCTCTTTGGTGTGGTCTGAATCTCCCTAGGGGCTTGGAGAAAATACTTGACCAGGCCATAAACTGAGCGAATTAAAAAGGAGCAGGAGAGCCGGGCAGTGGTGGCTCATGCCTGTAATCCCAGCATTTGGGAGGCAGAGGCAGGCGGATCTCAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGAACTTCGCAGAGAAACCCTGTCTCAAAAATCAAAATCAAAAACAAACAAACAAACAAAAGGAGCCGGATAGTTGGGAGTGGGCTTGGCACTAGCCAAAGGCCTTGAGACTCCCAAGAGCCTGGTGCTAGCCTGAGGGAATGGGATTTAGTGACCCATGCAGAACAGAAGGTGACCTATCGCGAATGGTACTGCGCTCTGGATTTCTCCGGCACAACCCAGCTGACTGCAATGTCCAGAATTCCTTCCCAGTCCTGGGTTCTGCAGGCCCAGCGTTTGAAGTCACCGGCAGGAGGGAGCCCCAGGTGGTCAACATCTGAGACCTTGCTGGGGACAGATGGAGTGCCCTGAGCACCTGTTGGATCCGGGGGAGGAGGCTTGTCGCGGCGGGCGAGTGCCCATTCAGAGGGATTGACTTTGTGCTGGGCGCCAGTGGAGTGAATGGCGTGCAGTGGTCAGCATACCCCCCGGGGCAGGCTGAGGGAGGGGTTCCCTATGTCCCCAAGGAGCAAGGGGACCTCCATAAACCCCGGAAAAAAGCGGGGAGCGCCCCCTGCCGACCTCCTGGACACCAGGCGCTAGAGAACTAATACCTTGGACCATTAGGTGCAATCTGAAATTTAGCATCACTTGAACATTATTAATGTTTTCGGAGAGCGTTGGTTGACTTCACACCAGACAGGTATCGGAAACTGACGTCTGCAGTGAGTGCGAAGGGTTCTTACTCTTTGCATACCCGGATGTATAAAGGTCTCCATTGACCCAGTTTATAATGCAAAAGCTCAGGCTTCAGCAATTCCAGCAAGGCTACCACCTGCCACAAAATATCAGTTAAGAGTTACAAAATATCAGTTAAGTGTCCATGGAGGCCCAAGCTTGGAATCTTGACACTTGGAAAGTTGAGGCAAAAGGATCCCACACAAGTTTGAGGGCAGCCTACGCTACAAAAACAAAACAAACAAACAAACAAACAGAGAGATTTACTCAGTGTGGCCACATTGGGAAGAATAAATAAAAAGGTCCAGTGAGTATTGGCCTGCGCCAGGTTTAAAGAGAAAAAGCTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGCTATTCATGATAAGGCCTGGTGAATCCAGTGAATCTGTGGTCTGGCTGATCGCTGCCACTGTTCTGGTTGTACAAGGTGGTCCTGGACTCCGCCCACCTCAAGGCTTGAGGGGCTCAGCCTGGGTCCTAGAAGATGAGCACTTGTGCCTCAGGCTGCAGCATGCCTTCCTATAGAATCACCCTTGTCTCAGAACTGGTTTGTTTCCCAAGCTTTGATCTTCCGGCAGAATTAGAATAACAACTTATCTCTGTGCCTTCAGCCAAAGGAAACACACACAGGGAAAGCAGAGATGCCAGGCTCCATCCTGCTTTAGGTACTCTGTGGTTACACAGAGAATCCTTGTCTCGAAAAACAAAGTCAAAAAGATACAAGAAAGAAAAGAAACTAAGATCTATGTAGACTATCGGTTTAAGGGGTGTTCTACAGTTCCTGGTTTATTATATGTCACTTTTAAGAAATAGGTTTTAAAAAGATTTTTTTATTAGGGGCTATAGAGATGGCTCAACTGTTAAGAGCACTGGCTGCCCTTGTAGACCACCTAGTTTCAATACCCCACACCCACATGGCCACTCACTCACTTGTAACTCCAGTTCCAGGGGATCTGATGTCCTCTTTGGTGTCCAGGAGCACAGACAGACATTCAAGAACATCACCTATACATATACAATAAGTTACATCTTTTAAAAAAATATATTAGAGTTATTCATTTTATGTGGGAATGTTTTGCCTGCATATATGTATGTCCACCACATGCATTCTTAGTGCCCCAGAGGTCAGAAGAGGGCACCCGCTGGAACTGAAGTTATGGGTGGTCTTGACCTGTCCTATGGGAGCTGGGAATCAAACCTGGGGCTTCTGTAAGAGCAGCAAGCCCTGAGCCATCTCTCTAGCCTCTGAAGAAAGTTTTTTTTTTTTAATTGCTGAGTATGGTTATAGACACCTGCAATCCCAGCATTCAGAAGGTAGAGAAACATTAGGAGTTCAAGGCCAGCTTCAGCTACATGGTTAACTCAAGATTACCCTAGGGGCTTCAGGACAGCCAGGGCTGTTACACGTAGACAGGTTGGGGGGCACAGATCACGCTGGGGATACTGGAGAGATGGCTCAGCAGTTAAGAGTGCCGGATGCTCTTCCAGAGGCCCCAGGTTCAATATCCAGCAACCATATGATGGCTTATATCTGTAACTCCAGTTCCAGGGGCTTCAATGCCCTCTCCTGGCCTCTGCCAGCAAGCACTTGCAGACACAGACACATCTGGGTGGATGTAGTGGTGAATGTCTTTTATTTCAGTGCTTGGGAAGCGGCAAATGCCTTATGAGTTTGAGGCCAGCCTGATCTACAAAGAGTTCTAGGCCAGGGCTACAGTGTAAGACCACTTGAGTTTGGAGCCCCCAGACCCACAAAAAGTTGGTATGGTAGTGGGTCTGAAATCCTAATACTCCAAGAAAGGAAGCAGAGATCACTAAGCATAGGGGACAGCTAGCCTGGCATATAGGACAGAGAAGCAAAGACCTCGTCTCAAACAAAGTACAAAGTGAAGATAAACAACCAAGGTTGTACTTTGCTTTCCACACACACACACCATGGCATGCACATGCTGTATGTACATAAACACACACACACACACGATGCTTTCCCTTTTGCATATAAAATCTTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCTGAAGTTCAAATCCCACATGGTGGCTCACAACCATCTGTAACGAAATCTGACTCCCTCTTCTGGAGTGTCTGAAGACAGCGACAGTGTACTTACCTATGATAATAAATAAATAAATAAATAAATAAATAAATAAATAAAATCTTAAAAATAAATAAATAAAATCTTAGCATATATGCACATTTGAAAGAAAGAAAACATCAAGTGTACCCAAGTCAGAACTTGAATAGGCCCAACCCCACATCACAACAAGCTAGAAATTACCCACAGAAGTAAATCAAATAGCAAGGACAATGATGACAGCCACCTTAGTTTTCTTTTTAAATAGCCACTGAGACCAAGAGAAAATTAAAAAAAAAGATAATGGTCAATTGTTTTGAACTCAGGTACACTACATGATTACATGTTAAGCGATAATAGCAATGTGCACCACACTATTCTTTGGGCCTTTGGTCCCCAGTTTTTAGTTGTGAAATTCCACAACCTTAAATCCATTCAGTATTGTAAGAAGAGGCAATCCAAATACGGAAATTAATGGTGCCAGCATTTCAGGAAATGAACGTGTAAAGGCTAAGAAAAGAAATTCGGCAGATATTGAAGTCAGCCTGTGCCCAGTCCTAAATTATAGTGTAAAATAGTAAAATCTATATGAGGTTTGCAATGGTGTGTCGGTGAGTAAGGCACTTACTGAGCTGCCTGAGGACCTGGGTTCAAATCCTGCTCACACAGAAAGCTGGACACAATAGTGGGTCTGTAATGCCAACATTCCTTCAACACAGAGCACAGTACTGCACATGGCCCTGAGAGAGTCCATTCTGCAAGCAGTTTTTGGCTCCATTTTGAATGTAAATATAGAGGCAGAATGACCTTGTACCTGGGTGGACAGCCGAGCAATGTCGCTTGTCTGTCACCACTTGTGAAACACAAACATGTAAAAAACATATTTCTAAATCAAAAGGATTCCACCCTATAAACTTATCAAAGTAAATTGGGATCATCATAAGCAAAGTAAAATTATACCAGAAAGAACTAGAAGGCTACTTCTGCCTAGCATCCACATGGCAGCTCACAACTCTGTAAGTCCAGTTCCAGGGGATCTTCTGGTCTCCCCGGGTACCAGGCTGCACATGATGCACATACATACATGCAGTTAAACTACTCATTTGCATATAAATACTTAAAAAACATATCAAGAAAATCAGGCCTAAGAACTTAGCAAACACATTAGACTAAAGAAGGGAGCAAGCCTGCTCCTTGGACCCCGTGAGGAGGAGGGCACTTCTTAACACGCGTTCACGCGACCGGCCAGGAAGAACACAGCAAACCAGAATCTTCTGCGGCAAAACTTTATTGCTTCCATCTTCAGGAGCAAGAGTGCAAACCCCCCATTGCTTACATCTTTAGGAGCCAGAGCGCCAGAGCTTTATTGCTTACATCTTTAGGAGCAAGAAGCAAGAGCCAGAGCAAGAGCAAGAAGAAGAACAAGAGCAAGAGAGAGAAAAAGAAAGCAAGAAAAAGAACAAGAACAAGAAAGCAAGAGTGCAAGAGCAAAAGCCAGAGCAAGAGAGAAAAAGAACAAGAACAAGAAAGCAAGAGAAAGAACAAGAAAGCAAGAAAGCAAGAGCAAGAACAAGAAAGCAAGAGAGAGAAAAAGCAAGAACAAGAGAGAGAAAAAGAATGGCAAAACCCCGTCCCTTTTAAGGAGAATTATCCTCCGCCTAGGACGTATTACTCCCTGATTGGCTGCAGCCCATCGGCTCAGTTGTCATCACGAGAAAGGCAGAACACATGGCGGGAAAACTGCCCCTGCACGTGTGCAGATTATGTTTACCACTTAGAACACAGCTGTCAGCGCCATCTTATAATGGCAAATGTGAGGGCGGCTTCCCACATATCCCCCTTTTCTTTTAATAAGAGCAATAGGCCACCCATATTAATGAGAGTGGAGATAGAGGTCAAATCCCCAGTGTGCAGGTAAAGGAGCCATGTACAGGATTAGCTCTTAGGCTCACAGGCTTTTACCCAGAGCAACCCTGACCTGCTCCTGTGTCGTTTTTCCTGGGGGAAGGGAACTAGGACACTGAACCTTCATGAAAGATGACATGTCTCCCTAGAATAGGCTCATATATGCCGCAGAGCCCTTCTACTGCAGTGCTTAGCCGTGCAACTCTCTCAGGCTGCTGAAGCACACTCACTCTATCCCGTGCAATGAGACTAGCCTCGTGGGGTGCAAGAGCTGAATGGCCAGCGACCTATTGCTTAAGCATAGATATATCAGGGGAAGCACCATGTTCTAGAGCTGCAAGTGCCTGGGCAATAACCACCTTGTCTCTCCTAGTTTGGGCCTTAAGCTTACAGACCAACCAGAGAAGCAACACTAATCCACAGCAAAGTGTATCTCCAAATAATCCCACCCATACCCATTCTTTAAAGAAGGAAAATGCTGAGGAGATCCAATTGGGTAATCCTTTGGTCAGGGACAGGTCCAAGTGCGTGGAGTTGACCTGAATAATGGCAATTCTCAATTCCCGAAGGGTCTGTTCAAATTCAGCCGTCCAATTCTGTAACATATACTGAGAAAGACTTTTTGACAAATTAGCTGCCCTAGTAAATTTCTCATACTGAATGGAAGTAACGGACAATGAGAGGTCCCCCAAGCTCCCGACAAACCCTGAGCCAGTCTTGTAAACCTTTGTTCTTTCTTGGGGCTATGGCCGCTCTGCATTCCTGCGTGGCTTGCTCATAAATGAGCTATTCTACCAGAGGTGCGGCTTGCTCCAAATCTGCAAAAATACGCTCTGCTGCCTCTGTCATTCTGGCCACAAAATCTGAGAATGACTCCTGAGGTCTCTGGAAGATCTTTGTTAGTTGTCCAGTGGCTTCACCTGCTCGGGAGAGCGCCTTCCAGGCCCTAATAGCCTTTTCATCTGATTCAGAACTATTAAGAACTGGCTCCTTGAGCTCATCTAGTGATGAGTATAGGCTCCTTCTCCTAGAAACCTCCGCTAATTGATCTTTTTTCTTTTCCTTCCTTCTAATCTCTCCCCAGGTATTCTTACCTGACCTTAACTTTTCCTCAGGTTCAAGACCCGTGGAAAGGCCTGTATACTTATTTTGTGCACCATATTTCTTCTTTGCTCCTACTCTCTCTCCCCGCTTTACTTCTGATAGATTGCCCTGAATTTCATCCAGAATTTTCAGCCCTGCCTTAACCACTTGATAACATGTGAAAAGGAACAAAAAGGCTCCTAATACTAGAGAAAGTTCAAGGCCAAACATACCTTGTAAAGCTATTTCCCACTTTACTTCTGATAGACTGTCTTGAATTTCGTTAGAAAGTTCAAGACCAGACTTACCTTGTAAAGCTATTTCCCACTTTACTTCTGATAGACTGTCTTGAATTTCGTTAGAAAGTTCAAGGCCAGACGTACCTTGTAAAGCTATTTCCCACTTTACTTCTGATAGACTGTCTTGAATTTCGTTAGAAAGTTCAAGGCCAGACGTACCTTGTAAAGCTATTTCCCACTTTACTTCTGATAGACTGTCTTGAATTTCGTTAGAAAGTTCAAGACCAGACTTACCTTGTAAAGCTATACTTACGGGTACCCTGTTCCCCAGCTGAAGAGTTCTGAATTCACGCAGTTGAATCCTTCTCAACAGTCTGTTTTACGGGAACACTTCATTACCACCGTTCCCCAGCTGAAGAGTTCTGAATTCACGCAGTTGAATCCTTCTCAACAGTCTGTTTTACGGGAACACTTCATTACCACCATTCCCCAGCTGAAGAGTTCTGAATTCACGCAGTTGAATCCTTCTCAACAGTCTGTTTTACGGGAACACTTCATTACCACCGTTCCCCAGCTGAAGAGTTCTGAATCCAGGATGGATCCTTCTCAACAGTCTGTTTTATGGGAACACTTCATTACCATGACCCGCAGTTCTGGTTCCGGAATGAGGGATCTTCCTTGCGCCGGTGATGGTTAACTCCCGTCCCGAGTTTTCTTGTCCCGGGTTTTTTCGTCCCGGGTTCTTTCTTCCCGGATTTTCTCGTCCCGTCCCGTCCCGTCCCGGGTTTTCTCGTCCTGTCCCGGGTTTCGGCACCAACTCTTAACACGCGTTCACGCGACTGGCCAGGAAGAACACAGCAAACCAGAATCTTCTGCGGCAAAACTTTATTGCTTACATCTTCAGGAGCAAGAGTGCAAACCCCCCATTGCTTACATCTTTAGGAGCCAGAGCGCCAGAGCTTTATTGCTTACATCTTTAGGAGCATGAAGCAAGAGCCAGAGCAAGAGCAAGAAGAAGAACAAGAGCAAGAGAGAGAAAAAGAAAGCAAGAAAAAGAACAAGAACAAGAAAGCAAGAGTGCAAGAGCAAAAGCCAGAGCAAGAGAGAAAAAGAACAAGAACAAGAAAGCAAGAGAAAGAACAAGAAAGCAAGAAAGCAAGAGCAAGAACAAGAAAGCAAGAGAGAGAAAAAGCAAGAACAAGAGAGAGAAAAAGAATGGCAAAACCCCGTCCCTTTTAAGGAGAATTATCCTCCGCCTAGGACGTATTACTCCCTGATTGGCTGCAGCCCATCGGCTCAGTTGTCATCACGAGAAAGGCAGAACACATGGCGGGAAAACTGCCCCTGCACGTGTGCAGATTATGTTTACCACTTAGAACACAGCTGTCAGCGCCATCTTATAATGGCAAATGTGAGGGCGGCTTCCCACAGGCACTGAAGCTATCAGTTGGACAAACCTGTCATCTGATCACCCAGAGTGTGTATCAAGGGCAGAGAACTCGAGACTGTCCCCAAGGCTTAGTCAGCTTGGTCTCCACTGCTGATTATGCCTACTCTCGTGGTTGTCTGTCTATCCATTCTCTTCCCTCCCCAGGTCTGTCTGGACCTACTTGATTCTCCACAGCCTCCACAGCCTCTACCATCCCACACCTCACTTTGGTGACTCCACTTCACCTTGAGGTTCAGCTCAAACCTGTGGCCTCCTGGCTTGAGTCTGCTACTGTGTCTCCAGTGGCCCTCCAGCTCGGTTCTTCCTTTATCTCCCACAGAGGCTTTTATCATGTCTACCAGCTTTTGTTTCTGCCTCTCAGGAAGGCCTTTCTAAATGCCGCCACCTTTGTAATCCTTTGTAACCATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACTATGTACATATGTGAATATGTATATGTATATTGTATGTATATGTGTGTATATGTGTATGTGTGTTGTATATTATGTATGTGTATATGTGTATATGTATAGATATGTGTATATGTATATATGTGTGTTGTATATGTATATATGTATATATGTGTATTGTATATGCATGTATGTGTCTATGAGTATATGTGTGTTTGTGTGTATATGTATATATGTGTATTGCAGATATGTATATATGTATATATATGCTTAGTGAGAGAGAGATGTTTACTGTGATGTGTTATTACTAGTAACTGAGACTAGAGGCAAAGAATCTGTATTGACCAATGGGCAGCTATCAAGGAAACAGGACTACTGAGCAAATCACCAGTACAGCTTGGAGAGATCATTGTTATTCAACCTCCTTGGCACTGTGGAACGACACACATGTATCTGTTGCTGATACAGCGGTTCTGTCAGGCTTTGCTTTTTTGTTTTTATTTTTTTATTTTTTAGTGCCAGGATCAAAAGCACAAACTTTTACATGCTGTGCAAGTATTCTATCACAAGTTAAGTCCAAAAATAAAAATGATGGTGATGATGATGATGGTGATGGTGGCGATGGGGGCGGGGCAGCAGTGGCAGTGGCGATGGTGGTGGCGGTGGTGGCGATGATGGTGATGGCGGTGGCTGGAGAGATAGCCTGAGCAGTCTTCCTGTGGTCCTCTCTTCTCCCCGGCCTCGTTGGCCACTGGCCCACAAGAACAAAGAGAGCAGAGTCAATGGACAGGATTTCAGATGGCCCACAGGTATGATGGAATGACAGACTTATCTCCAGGCTCAGCTTCAGAGAGACAGCACAACTTTATTCAGGGTGAACCAAGGGCTATGTAATTTTGGGAAGAGGGCAGGTATTTCCAGGGAAGGTGAGTATCACTGGCTACATCTTATGGAACCTCATGTGCATGGAGAAACATTCCAGGAATCTCAGGTGCTGGCTGGACAAGTTCTTATCCGAAGGAAGGAAGCTGGACCTGTAATCTCAGCAAGGCTGTGTGACATTCCTCGGGTAGGGGGTGTGAGATCTGGGATCCCCCAGGTGATAAGAAGGCTCACTGACGCTGATGAAGAAGTCTGCCATTTTGCACCTAGCTCAGGGCTTCCCAGTAATGTCAGCCTTCGCCCTTAGCAGCTTAGCAGCGGGACTTCCTGACACTT
GF3 -1 -1 1 GCAGAGCAGAGTTGAGCCTCAACGTTGAG
GF4 -1 -1 1 GCAGAGCAGAGTTGAGCCTCAACGTTG
EX52 -1 -1 1 GAAGTGGGGAGCTGTGGTGGTGCAAGAAGC
EX32 -1 -1 1 CAAGGAAGCAGCTAATTCAGACACCTTC
GR3 -1 -1 1 CAGTTACTGGTTCGAGGCAGAATAGTCCG
GR4 -1 -1 1 CTGCTGGTTGGTAGTTAGTTCAGAGGCCTG
Comments:
Target: 2803Ensembl ENSMUSG00000079445 -- 88201620 -- 88203878 ENSMUSE00000695113 -- ENSMUSE00000695113
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:1:1:197195432:1, start 88201619: end 88203878
Found 9 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:1:88195120:88196119_831 G3:1:88207378:88208377_931
ACCEPT (0) gap pair G5:1:88195120:88196119_831 G3:1:88207378:88208377_917
ACCEPT (0) gap pair G5:1:88195120:88196119_831 G3:1:88207378:88208377_612
ACCEPT (0) gap pair G5:1:88195120:88196119_837 G3:1:88207378:88208377_931
ACCEPT (0) gap pair G5:1:88195120:88196119_837 G3:1:88207378:88208377_917
ACCEPT (0) gap pair G5:1:88195120:88196119_837 G3:1:88207378:88208377_612
REJECT (2) gap pair G5:1:88195120:88196119_490 G3:1:88207378:88208377_931
REJECT (2) gap pair G5:1:88195120:88196119_490 G3:1:88207378:88208377_917
ACCEPT (0) gap pair G5:1:88195120:88196119_490 G3:1:88207378:88208377_612
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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