High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
 
       
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by mp7
Valid After Annotation
not done
Comment Type Comment Detail Edited User Edited Date
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 116342
Chr: 5
Strand: 1
Status: Ordered
BACs: RP23-389E8 : RP23-467N9 : RP23-177G13 : RP23-114C5 : RP23-30O19 : RP23-377P1 : RP23-418E13 : RP24-138M23 : RP24-274M3 : RP24-470H10 : RP24-237P18 : RP24-211H21 : RP24-245K1 : RP24-443I16 :
Instance: Plate 160: Well G11: BACs RP23-114C5 RP23-389E8 RP23-467N9 RP23-177G13
Oligos on GRCm38:
Type Start End Length Seq
G5 118252750 118252799 50 GTCTCTCATTTGACTGTTATGATTACCCATAAAAACTAACACAGCCCACT
U5 118255199 118255248 50 CTTCGTCTGGTCCTGAAGTCATCCCCAGTCCCATTTAGAAGTTTTGTATG
U3 118255314 118255363 50 TAGAATTACATAGTTTCCTCCTGGTTGTAAAAGTATTTGGGCTTTGTGTT
D5 118255987 118256036 50 TGAACACCCAGACTCCTAGCATTTCCCTCTGCCTACAGCACCAGCCTTAG
D3 118256051 118256100 50 CACCGCCTACCAGGCACAGGCATGGGGTTCCTCATCTTGTCTCTCTAATT
G3 118262728 118262777 50 ATATTTAGAGTGTTGACCCAAGTTTTAGTATGAGCAGTCACATCTGTTGC
Oligos:
Type Start End Length Seq
G5 118702759 118702808 50 GTCTCTCATTTGACTGTTATGATTACCCATAAAAACTAACACAGCCCACT
U5 118705208 118705257 50 CTTCGTCTGGTCCTGAAGTCATCCCCAGTCCCATTTAGAAGTTTTGTATG
U3 118705323 118705372 50 TAGAATTACATAGTTTCCTCCTGGTTGTAAAAGTATTTGGGCTTTGTGTT
D5 118705996 118706045 50 TGAACACCCAGACTCCTAGCATTTCCCTCTGCCTACAGCACCAGCCTTAG
D3 118706060 118706109 50 CACCGCCTACCAGGCACAGGCATGGGGTTCCTCATCTTGTCTCTCTAATT
G3 118712737 118712786 50 ATATTTAGAGTGTTGACCCAAGTTTTAGTATGAGCAGTCACATCTGTTGC
G5_U5 118702759 118705257 2499 GTCTCTCATTTGACTGTTATGATTACCCATAAAAACTAACACAGCCCACTTCCTAATTAACCTCCTGAAAATTTGAGGAGAGGCTATGTCCATTAGCTATGCAGGCTACACAACTGTTTACAGAGAACATTCTCTTGTGCTCCCCCCACTCCGGATCCCATCTTTCCTCTGCAGGATCTGTGGACAGCTAACACCTCGAGGGTTGGGTGGATCTGATTCACACGGAAGCCTTTGTCAGCAGCCCATTGGAGCACTTGTCTAAAGTGTCCATGGCACTCTGTGAAAATCCCCTTTGAATGGATATTTTTTCCTTGTTGTTGAGACAAGGTTTCTCACTGTAGCCCTGGCTGTCCTGAATCATCTCTGTGTAGATAGACCAGGCTGGCTTTGAATCTGCAGAGATTCACCTGCCTCTGCCTCTTTGAGTACTGGATTAAATATTCGTACTGCCACGCCCAGCTTTGAATGGGTCTGGAATGAAGAAAATCATATACTTGGTGAAGTGCTCAAAGATAAGACAGCCCAGAGGGAACTCAGTAGGGAAGTCCAGTAACCCACTGGGTGGAAGAAGATCTCCTGTCCCTTCCACCCTGCCTCCAAAAGTGCACCACAGCCACGATTTAGCCACGGTAGAGGTTTCGAATATTCATGTTTGACACGTTCAGGTGCTGGAGAACTGCCCTCCTGTTCACCAGGAACAGGGTCCATGGTTGAGGTCCTTCTGGGGTCCATTGCTCCAGGCTTCAGCTAAGGTCTGCTCCTGATCCACAGGCTGCGCCTAAGGGGGCCCCTGTCTCCCACGGGTTCTTGCTCTCAGCCCTTCTCTTTCTGTAACTGAGGTAACAGCTACCTGTCCTGTCCTGTGCCACCTAGCCATAGCCTTTAAAAGAACCAAAAAAGGAAAGGCAGGCCACAGGAAAGCCATCTTGAAGAGACTGTTTTTACATTGAGCAAACTGAAAAAGGGGAACCTAGACCTGCCGGGCTTGTGGTAGGAGAGAGGCCAGCTGATGATACCTCAGAGGGTTCATCAAACTTTGGGGAGGCTAGGCACTGTCTGGCACTTCCATCTGCAAGTGATTGACAGGACTTTCTCACACCCCCACACCGAACTGCAGGGTCATCCTGCGTGCAGAACCAGGGGGCTGTTGCTGGCCTGTGGAGGCTGTGTTCTTAATGATGGCACATTGACATGGTGGATCCATGGCAATGTGATTATCTAGATAACGTGGGTCCTTGGAAATGCTTTACTATCCACTTAGTGTTGAGATCAGCCGATGGTGACTCCAGTATGCCCTGGGTCAGAGGTCACAGGTCAGAGGTGAACATGTGGGGAGGTGTCATGAGAACTTAGCTCTGATGATAGTGTGTCCCTGCTAGTGTTTAGTCAGACATTTCCGTGGTGTTGGGTTTCTAGGTTTTTGTTGTTGTTTTGGGGTTTTTGTTTTGTTTATGTTTTTCCTACCATTGAAACAGAGAGCTATGGGAAACCTCCGTGTATGGTTCCTGTGGGATGGCACTCAGTAAAGTTTCCTAGAGCAAGGTTTATGCATATTCAACTTCAACAACTTCCCAGATTGCCTTCCCACAGTAACTACAAAGATCCTGTCTGTTTCCCTGAATTGTCTGTGTTGCCATCCGATGCAATGCGCAGGGTCTCCATAGTTACTGAGCAACATTATTGAGGCTTAAAAAACACCAGCCATGGGGATAGCAGGCGTGGTGGTGCACACCATAATCCCGTAGTCCCAGCAGCACCTGGCAGGCTGTTGCAGGAGGACCTGCGGCCATTTGGGGTTGCAGAGGGTGACCCTGCCTTAAAACCTCCAAAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCCACTGCTCTTCTGAAGGTCCGAAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCACCCGTAATGAGATCTGATGCCCTCTTCTGGTGCGTCTGAAGACAGCTACAGCTAAGTGTACTTAAATATAATAAATAAATAAATTAATTAATTAATTAGAAAAAAAACAAAAAACAAAAAAAAAACCTCCAAAACCAACAGGCCATGGTGCCTCACACCTATAATCACAGCATTTAGGAGGCCGAGGCAGGAGCATTGCCATGAGTTTCAGGCAGGCCTGAACTACAGGATGAGAAGCGCAACAGGAGAGGGCCTTTTAGAGGTCTTTTGTTACACATTCATGGCTAGAAGCTTCCAAAGATGTGACCACCTTGTGCTTCTCTATCTTTCCAGCAGCTGGGCTTCTCCTCCTAGCTCAATGGTGTGGATCCTGTAGGGATCCAGGCTCTCACCCAGGGGCCACTCCTCTCCTGGCTCAGCAAGGCAAAGGGCCGTGTAGGCAGAAAGGCATCTGAAAGTAATCATAAGGGTGACTGCTGTGTGCTGTCACACAAAGGATGAGCCTTGCAGGTTGTCAGGCTTCGTCTGGTCCTGAAGTCATCCCCAGTCCCATTTAGAAGTTTTGTATG
U3_D5 118705323 118706045 723 TAGAATTACATAGTTTCCTCCTGGTTGTAAAAGTATTTGGGCTTTGTGTTCTGTTCCATGGTGTGTGTATTAGTATCTAAAAACTTAAGTTGCTAGCAGAGAGAAAAGTTTTTGCTCCCCCCACCCCACCCCGGATTTCACAGAATCTTTTGTCTGCTTCTGGGGCCCTGCACATCAATGCAGTAACTGGGGAGAGTAGCTTTAACTTGCCAGGTTTCCCTGATGACATAGATGGCACCTGTGCCCAAGGGTTGGGTCGAGGGTAGAGAGATGGAGGTATAGATGCCTTTAGGGCAAGTGAGAAAATGCAGGAAAAGCGCCTTGACCCCTCCTCTCTCGCTGTGGTCTCCTTGCAGGAAGAGAGGGCTGTGAGAGACCGGAACCTCCTCCAGGTTCAAGACCGGGAACAGCCCATCCCGTGGAAGGTGCAGTTTAACCTAGGCAACAGCAGCCGACCCAGCAACCAGTGCCGGAACTCTGTCCAAGGAAAACACCTCCTCACTGATGAGCTGGGTATGTCTCCCTCCCTGCACTCAAGGTGCAGAGACTCTGGGTTTGATAGCCTTCCCTCCCAGTGCTTTTGCCCTTCTTGCTAGTCTTCAGGGATTTCCCTGTCTGTCTTTCACACTCTCCTCTCCATCCCAGGCTCCCTGCAGCATCTGGGAGGCTTGGCTGAACACCCAGACTCCTAGCATTTCCCTCTGCCTACAGCACCAGCCTTAG
D3_G3 118706060 118712786 6727 CACCGCCTACCAGGCACAGGCATGGGGTTCCTCATCTTGTCTCTCTAATTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTATGTGTATGTCTGTGTGTGATATGTGTGTCTGTGTGTATATGTGTGTCTGTGTGTGATATGTGTGTCTTGTGTATATGTGTGTCTGTGTGTGTCTCTGTGTGTGTGTAGTCTGTGTATATGTGTGTGTATGTGTGTGTCTCTCTGTGTCTGTGTTTGTGTGTGTATGTGTGTGTGTGTCTGTGTGTGTTTGTGTGTGGTCTCTATGTATATATGTATGTCTGTCTGTCTGTGTCTATGTGTTTATGTGTCTGTGTGTGTGTGTGTCTGTGTATGTCTGTGTTTCTGTGTTTGTGTGTGTGTGTGTGTCTGTTACACCATGTCATGTATGTGGCAGTCAGAGGACAACAGGCTGTTTTAGTGTTAGATGGCAGTTTTTATCATAGCTATAGCTACAAATTGTTAATTTAGGTCAGACAAAGACTCACACTCCTTCAGCTTCTTAATCAATAAAAATCCCACAAGGGCAGCAGGTTTTACTTTGCCATTATAAATATGGGGGAAGTGGTTTAATAAAGAAAACTTGCAACTGTTGATGCTGTTTAGCAACGAGATGCCACCGTGTCGCCAGGGTAACAGCACTGTTAAACAAGATAGCCAATTAGAGTCACAAACTTTTCAAAAGCTGGAAGTTAATTTCCCAGGCAAGACCAACACATTTGCCTGCCCTGGTCTCCCAGGACTTGGTAAGAATAACATCCTTAGAGCTGGTACGCACCACAGGCTTTCTCTGTGGGGCCCTGGCTGTACTGGGGCTCGCTCTGTCGACCAGGCTGACCTCGAACTCAGAGAGATATGCCCATCTCTCCTGGGCGCTGGGATTCAAGGCATGAGCCACCACACCGGCTGGTGTATATGTGTTTTGAAACATCATGATGACCCAGTTCTATGTATGATGTGTGTGTGCGTGTGTGTGTGTGTTTGTGTGTGTGTATCAGATAAGAAAGGTTGGAGAAATGGGTGTGTGGCCAAGAGCACTGACTGCTCTTTCCAGAGGACCTGGTTTTGAGTCCCGGCATCCAGGACAGGCAGCTCCCTCTTCTGGCCTCTGCAGGAATCTGCACTCACATGTACTCACACATCCAATTAAACATAAGGATGACTTCAGAAAGTAAAAGGTAATTTTTGCATGACACATGGGGAGAGAGGCCTGGGTGAGCTTGCTCTCTCTTCCCAGAGATTAGACCTGGTCATGGAGAGGTGAGTACCAGAGTTCAAAGCCGAGATCTGGGAGTTGGTAACGGTAGGACCAGGCCTGAGATGTCAGCTTCCAGCTTCCCAAAGGAAAGGACAGTGAGTGTCAGTGGGTGATATCGGGGTGTTAGTGTCAGCTGGTGTTATTGGGGTGCACAGTATTCTTGACACAGACGGAGTTGCTGTGTTGCTCTCATCCAGGGTAGAATCCTGAGGAACGCACGGGCTCTCTGGGAATTCTGTTTAGAAGCCTTGTCAACAGCGTGAGTCAGGGCATCTGGGAAGGAAGTGTTTACCAGGACATCAGAATGTTCATCACTACCCGTGAGATGCAGCAGCAGCAGCAGCAGCAGCAGAAACAACAGACGCCAGCCTTTCCCTGCACTGGGAGAGGTTGGCGTCTCCCCTGGTCTCCCAAGTATTGAGGGCCCTCTGGTGTCCCCGATCGCCAGTGTTCTGCCAGCTCCCACACAGACCTTGTTTTCCAGAGCAGCTCTAAAAAACAAGTCATCCTCCGCCAGCCTTGTTCTCTTGAGGACAGAGCTGGGGTGCTCAGACTGGCTGGTTGGGAACAGCTGCTGCACAGCTGCGGGCCCCTGCACACACCCGAGGGGCCCCCCATATTCACCAGAAGGCCTCTGCACACACCTTAGGGTCTACACACACACATACACCTGAGAGCCTCTGCACACACCCGAGGGGCCCCCCATTCTTAACAGAAGGCCCCTGCACACACCTTAGGGTACATACACACACACCAGAGGGCTCCTGCATACACCCGAGGGTCCATACACACCTGATCCCCCCCCCCCCCCGCCCCCGCCGCACCTCCCCATACACCTGAGGCTCTCTCACATGCACACATACCTGAGGACCTAAATGGCCACTGGGGGCGTGTTCGAGGTTGGGTGGTGGCCCTCATAGACTTACATATTTTTCTGTCCTGTTCCTCACAGTGCAGGGAACAGTCTGCCTAGAAGCCCTGAGCAGTTAAGTAGTCTCAGTGAGGGAGCCTGACTGACATGGCGCTCGATGTGCACATATCTACGTGATGCAGTTTCACAGTTGTGGAGCGAATTAGGTTTTTCTCTCTTTTGAGGATTTGAGAACACCCCTAATTCTAGGGGGGTGCTTGTGGTTGTGTCTCCCCCTGTTGGTATGCAAACACCGGAAGCAGAGTCACGAAGGTTGACTGTGGCCTCCTGTTTTAGCAGCCCCTTCAAATGACCACAACAGCCACGAGCCATCAAAAGCCACATGTTTGCTTTTTAAATCCGCAAGGCACTTGTTTTTGTGAATGGGTGCCTCGTTTCCTGTCTTGGACTGTTTTGTTAAAGCCCAGGTGAAAGGCTGCGTGCTTCCATTAGCATCCCAGCTGCACAGACAGGGCATGGTCTGGCAAGAAGCTCACACTCAAGCAGTAGACTGGAAAGGGGCGGAGCGTGGTAACAACTCAGATCTATCGATGACTGAGGGGTCCAACTTCCCGTCATCCTCTGCCCAGGTGAAGCCAGCCCTAGACACGTTTCCATGTCTGGTCCTGATGCTCAGGGTGCCCTGGCTCCTGCCTGCCTTCTTCCTGTGCAGTTCTTCATCCTCAGAGGGAACACAGGTGGCTAGCTTCCCGCTGAGCTCTGCCTCTGGTTTCAGGCTATGTCTGCGAGAGGAAGGACCTGCTGGCGAATGGCTGTTGCGACGTCAGCGTCCCCAGCACAAAGCAGTACTGCTGTGATGGGTGCCTGGCCAATGGCTGCTGTGAAGCCTACGAGTACTGCGTCTCCTGCTGCCTGCAGCCCAGCAAGGTACGGGTCCCATCCCTTGTGTTCTGTGCATTTTAGGGGGGTAGCTAGGCATCTCCTGCAGTGTTCTGATGGTGCAGTGGCCTCTCCCGAGGGAGGAGCTGGTCTCCCTGCCTCACAGTTCCTTCTGCAGAGAGGACAATGCTATTGCTAACTAGGTATCTGGTGAATGAGAGAGACCTGGCTCCTTGCCCTCCCTGTGGGCCAGCTCGGCCAGCACTTACTAATACCAAATGCCTTGTTTCCATAGCAACTTCTCCTGGAGCGCTTCCTCAACCGGGCAGCTGTGGCCTTCCAGAACCTCTTCATGGCAGTTGAAGACCACTTCGAACTGTGCTTGGCTAAATGCAGGACCTCCTCCCAGGTCAGAAGTGGTGCTGGGGATGGAAGAGAGAGACAGAGCTGGAGAACCCTAGCTGACCGATCTGGGTAGAGCCAGGGAGGAAAGGGGCTTCATTGACGTTCTGCTGTGTTAGGGGGTGAGAGAGTTAGATCCAGTACCAGCCATTGAGAGGAGGCAGTAGTACTTGCTCTAGGGCAGAGTTGTGGTTCGAATTAGGATCCAAGGGGATAGGAGAGTCAGGCACATGGATATATCTGAGGCTTCTAGGGTGGGCACACACTGCCTGCCATTGACTGTCTGTTCTCATAGCAGGGAGTGCGCTGGAGGGCCTCTGCCACCATCACCAACCCCATCTCTGTTTATCTTCCCTTTTCCGTTTATTCTGGGCTCAGAGCCACAGACACAAGTCAGAGGACAACTTGTGAGAGTCTGTTCTCTGTCTACACCAATGTATATCTCGAGGACCAAGCATTGGCAGCCAGCACCTTTCAGGCTAAGCCATCTTGCCTACCCAGTCTTTCTGGCTAAGCGCTTGTCTCTGCCCTTTCTCAGACAACCACACGTCTCTTACACAAGCACACCCTTCTAGGATACACTCCGGCTTTGTGCTCGTTAATCCCCCTTCCCATCTCTGGGACAGGATAGAGGGAGCGGAGAGAATGTGAAATTCCACTGGTCTCCATGAGGAGGGGGCTTCTCAGGTGAGTGGGCGGGAAAGGAAGTCGGGTCACTTGCTCTTCCTGACCCACCCTGGCAAAGGGAACTGACTAGGGAGGTTCCTGCCGCTGTCCAAGCCGCAAGTCACTTCCTCTTGTGCAAATAGAGATAGTTCTCAATGGCCACTTGGTTCGAGTGCCCAGAGCCCAGGCTGCGAATCTCTTAGGTGGAATTAATATGGATGTATGGCTTTGGTAGGGGATAATTAATGAGCTCTAGACCAGCCAAAGCCTCAAATTGTTTGTATGAGAAGAAATTGCTGGGGGCGGGGGGATCTGTCAAGAGGGGTGGGGACGGGACATCATCTGGGCTCAACCTCACTTGCTGTGCCCAGCCAGGGCACTCTTGGGGTGGATGCTTGACTTGTGCACAGGCCCTTAGGGCCTGACTGCATTTTGACGTGTCAGGACTCCGCCAGGGACTGGGTCTGTCCAACCACAGGGTCAGCGGCCGATCTCGCTGCTCCACCGTATCTCCTTGTCTGTTTCAGATTGATTTTGTTTGTCTGTTCTCTTCATATTTACTATTGTGGCCGGGACACGCCACCATGTGCACGGGAGGTCAGAGAACAGCAGCTCGCTTCTCGTTCTCCTCTGTGGGTCCTGGAGATGGAGCTGAGGCTCTCAGACTTGGGAGCAAGCACTGTGTTAGCCCTGGCCATCCCATCTGCTCTTTTCGGATGGATGGAGCAGTCTCAGAATCTGCAGCCCGCAGTGCGCCTGTTGAGAAGCTCCCTGGTGGGCAGAAGAAGAAATGGCACAGGGATGTCCTATCTATTTGGTGGCACTCAGAGCCAACAATATCCTACGTCATGCTAGAATAGTACCTTCTAGGCATGCTAGAGGTCTGGCAGGGACTGTGGCTGTCACTGCAGCTGACCCTCTCTAACACACCCCTGTCTTCCTTCGCCAACAGAGTGTGCAACACGAGAACACGTACCGTGACCCCATAGCCAAGTACTGCTATGGAGAGAGCCCGCCTGAACTCTTCCCGGCGTGAGGCCTGACGGAGATACACACCCAGAACCAGGCTGGTCCACTGGAGTAAAGAAGGAGAAAGCCACACAAGGGTCCTTGTGGCTTTGGGTGTCTCCAGCTCCCTCACCGACAGCTGGAGCTGATGGGCGGAGCTGTTCTGTAGAGCAGGCTGGATGAGGTCCTGGCACCTTGCAGGCCCCTGGGACTGCTGGTCAGAGGCCAGGCACTTGCTTCTGTTTTGCTCCTACCTTGTAGTTATTTATTGACTTTTCAGAGTGGTGGAAGGTACGGTCATTTTTTAATTTTTTTGGTGCAGGACACCTCTGCCTGTCCAGATGAATACATTGGAGGAGGTGACCGTTGCTCTCATTTCCTGAGCGTCGCATGGATGTGCGTGCATGCTCCTTCACCGGATGAAAGTTAGTCTCTGGGAGTGCGGGTGTACAGACTCAGTTGTGGTTCTGTTTTAACATCTGACCAGCAGGCCCTTGTATCCATACCCCAACATTCAGGGGTATCTGAAGCCTCAGGGAGGTGCTGGTCCCCACTGAGCCTTCATAGAGCTGCCTCAGTGTGCTCATATACTGAAATGGTAGACAGGAAGCCTGAATCCCAGGCTGACTCTCCCAGTGGAGTTTGCTGTGTCTCCTTGAAACCCCTCATCCTCAGGGCAGGGGAGCCCTTCCCTTGTCTGTCACAGAGGCTAGGGGGACTGCGACTGTGTGCATGCACAGTGGCCATTCATTCTTGGCTGCTCTCCACGCCCGGTGGCATTTATAAAGAAAAGCCCCAGCTCTTAGTCCAATCTGTCGCAAGTTTCACATCCACCATCAGTGGGAGACGAATGTGGTAGCCACAGTCTAGGGAGCGTCCCAAAGGCAGGTCCATGTCCAGTGCTCACAGGACAGTTGTTACCAGCGACTGAAACGGAGGTTTGAAGCCTAAGGTCAAGACACCTCCTCCCGTACTCTCCTGTCCTGTTTACAGGCCAGCCTTTAGCACACACTTCCTCCCAAGACCTTTGACCCACCCTTCCAGTGGACCACGGTGTTTTGTGGTTTTGGTTCAGGTTGACCTGGGATGGACGGGCCGCGAGTCTCAAGATTTCCTATCGGGGCCACATGGAACACAGCTCTTTGTACCGTCAGGATGGAATTTCCCTCTGCTCCCTCCTGCTTCCTCTTGCTGTTCACATCGGATTTGTATTCTCTCAGCTCTACAGAAGGACAAACTGACTGTCAGTTGCTCCCTTGTTCTGTCTTGCCATGCTGGGATCGAACCCAGGACTTTGTGTGGGATTGGCAAACTTGCTGTTCCTGAGCCACGGCCCTGGCCAGGGAGTTGATTTTTAGTGTTAGATGCCAGTGATTCTCTTCTGTTTGCTCTGGACTTCACGGTGTGTTGACTCAAACTTGATAGAAGAACTGGGTGATCCTGCACGTTCTGGCACATCCTGGAATGTTCTGGCACGTTCTGACATGTGCTATATGCGTTTGACACCAGCCTTAAACTCCTGACTCTCCTGCCCTGGGAGGAGTCCACCCTTGGTTGCCAGTGGCATTGGTCATATTTAGAGTGTTGACCCAAGTTTTAGTATGAGCAGTCACATCTGTTGC
U5_15 118690209 118705257 15049 CATCTGAAAGAGCCCATGAGATGCATTCATGAGTATTGAGTATCGGTGTCTGCAGACAACTGGCAAACTGAGTTTGATACCTGGCGAAAAAAGAGAACTCCCACAAGCTGGCCTACATACGTGCCATGACACAGGCACCCCATTCACTGTAAGAACACACCCAAGGCAGCTGGCTTTATAGAGAAAAGGTTTAAATAGATCACAGTTGTTAGCATTTTGTCTACACTCCGCCCCACAGTTACCTGGCAACAGCCATGTGTGCCTGACTCTCAATAAAAGGGGCTGCTTGCCCCCTCCTCTCTCTCTTGCCTTCTTGCTCTCGCTCTGTCCTCTTGCTCCTTACTCCTTCTCTCCCCATTTCCCTCCCCCTCTCTCTTTGCACCACGTGCTCATGGCTGGCCTCTCTCTCTCTCTGCCTTTCTGTCTCTTCTTCCTTAACTCCCCTCCCCATGCCCTGAATAAACTCTATTCTATACTGTCATGTAGTTGTGTGGCTGGTCCTTCAGGGGGAAGGGATGCCTCAGCTTGGGCCCGCAGAGGCACCCCTTCCCCCCACACCTGACCACACATGCACCAAACATATCCCTTCTCTCTTTATCTTTTTATAAGAGACAACAACAGTCTTTGAGGCTGAGAGAGCCAATGGCAAGGCCGGCTCAGTGGTCATATTTGATTATCACCTAGTGCTGTGGCTGGCAGTGGCGGCTGTCATGTAAAAACTGGATAGTTTTAGCAGTCGTCTGTGATACCAATAGCTGGAGGCAAATACATGTATAATGTAAATGAATAAATGTAATTAAAATTACAAATAATTAAACATTACAAATAATGTAATTTTACATTATAAATAATGTAATTAAAAACTATAAATAAATAATGTAATTTAAAGATTAAAGTACAAAGTTTGATAAGGAACAAATGGACCAGAGATCAAATTTCAGCCCTGCCACTTCCTGGCTGTGTGACTTTGCTAACGGACCTGCCCTCTCGAACATCTTATCTTCAGGGGGCTCTTAGGGAGGTGTGGCGTAGCCCCTCAGTGCACGGTGGGCAGTGTTCTATCTGCCATTGTCTCTGTCCCCAGAGATATCCGTCTGGGTTCAGTTGAGAGCAAAGCTGCCCTCAGCTCAGAAGATGCCACCGGGCTTGCCAACCGAAGGACAGGTGCTGCGTGCGTGTCCTTGGGCTCTCGAATACACTGTTCCACCAGGGACTCTAACTTCACCAGTATCACCTCTCTACGGCTGGAGAGTCTGAGACCTGGAGGTTGCTGCTTCCCATGAAAGGCACCACAGCTGGCGCATCTGTTCCCTATCCGTGTTCACGATAGATGCCTGAGATTGGATACTGGTGTACAACACAACACTGTCCTCTGGGACTGATGTGGCTTCCGGAAATCAGAACAGCTGCACACACTCCTCACAAGACTGGGACACAGAGAAAGAGCGAGCAGGTGGGAGGTCATCAGACCCCGTCCCCTCCCCTGCCTCATGCACCTCCACAGCTGCGTGTACGTTGCCCCAGGAAGACCTAGACAGTTTTCTCCAGGGAGGATGCCAGGGTGAGTCTCAGCCCTGCTGGGCTGGGCTTTTTGGGGATGCCGCTGCCTTTGAAACACTCATTTTCCTGAAAGTGACCCCAGTGATCACATTGGTTCACACAGCAGGCAGACAGCCATCCAGGAACCAGCTCTCACCAGGCACCAATCTGCCAGCTCCCTGACTTTGAACTTCACAGCCTCCTTACTGGTCAGGGATGCTCCAGTAGGCAGGCCAGACCCTGTGGGGATTGCTGCAGCAGCTCATGGAGGGAGGCCAGGTGTCTCTATATGTGAAAAACTGGCAGGAAGAGTCTGAGTCATATAGATCTGTCTCACAAGGTTTGACAACTGTCCTAAGACAACAGCACTTTATCCACTGAGGCTACCTGGACCTCTCCATCGTGTTTGAGAAGTAAATCACACTCACCCATGCATGCAGCCGCCACTGCCAACCACGCCACTAGGTGATGAGGTCAAATATGGCCACTGAGCTGGCCTTGCCTTTGGCTCTCTCAGCCTCCAAGACTGTGATCTACACAGACCTTTTCTCTTTATAAAGCCGGCGGCCTTGGGTGCGTTCTTATAGGAAGGGGGAAGCTGCCAAATACACTGCAAAGCAATTCTCCATTGGGTCTGTTTTATACCCCCAATAACTAATATTGAAAAAGCTAGGGTCCCCAGATGGGAGGAAATTGGGGAGGGGGGCAGAGACTCTTACCTTTCAGGTGGAATGTTGTCCGTGTTGCTACTGTGGCGTCTCTGCATTTTCCTTAGCACCTGAAAGCGACACACACGTGTGAGCTACATCTCTGGACACGCTCCTTCCTTGCCTGTTGCCATGGTGACTGTAAATAAGTACCCCATCTCACAGTGAGAATAGAGAGAGTCACAGGTGGAGGTGGCTCCTACCCAAAAGGGAGAAAACAACAAATGGGAGCAGACAGGTGTTTGTTGGGTGTGGTGAGCAGCAGACACTGCCCCCTTTAACTTGCTATGTGGCCTCAGACAAGCCAGGAACCTCTCTGTTTCCTGGGGAGAGAATGGAGCAGTGGGACCCAGGGTTCCATCACTTCATAGGTGAAGGTACAAATCCCTAAGGAGACACGTCACCTGGTGCGCAGCAAGGAGCCACATGGACCTCAGAACTCCATGCTGACGAAATGTGCCGTGGACACTCAGGGTTCCTGATGGGATGTGGCCACTGGGTCCCAGAGAGAGCAGGCCTCCTCCTCCTCCTCTTCCTCCTCTTCAGAGGGAAGCACAGGACAGGAGACTCATTGGAGACAGCAGACCTTGGGTGGTACAAAGCACTTGTGTGAACAGGCCTTGGGATGACTTGTTACAGAATTAGCTGTCGGATGTGACTGGGTACTGTAATAGCCCAGTAGTTCTGGTAGCCTTGGAGCTATCCTCCCTGAGGTGCTGATGGTGGGTTATGGCCTTACTAGGTTTGTCCTATCAGCAAACTATGCATCCTGAGGGTTGGAGAGAGAGCTCTGTGGATAAAGTACTTGCCACACATGCACGAGGACCTGACTTTGATCCCCAGCACCTCTGTAAGTAGCCAGGCATGGTATTGCACTGTGTAATCCCAGCACTGGGAAGGCAGAACCAGGATTCCCAGTACTCCCTGGCCAAGTCCTTCCAGCTGAGGAGCCCTAGGTCCCAGTGAGAGATTCTATCTCAAAAATAAGGTGCCGAGTTCTGGCAGAATGACACCTGGGGGTGACCTCTGACCTCCACGGACATTTGCAATGGCCCCCCCACCCTGAGCATCCATACATACCTACATATGTACATATGCATGTGCACACTTATACACATACACGCACACGCACGCACAGATGTGCACAAGCACACACTGGGGGGGGGGCCATAAAAATGATTTTAGAATTTCTACTGGCTTCTAAGTGAAACCAGAATAAAAATCAGAGTCGTTGGAGGCCTCCAGGCTGTGCTGGACGTTATCATTACCCGTTTACAACCTTGATTCTGTGACTTATCTCTGGCCATAAGAACTCCAGGTACCTCTGGACACTTTAAAGTTAAGAGTTCCTGCCTCCGGGCCTTTGTACCTGCTGTTCCTTCCCTGGCATGGTTTTCCAGCTGAGGGTTACAGGCTACCTCCCCACAGCACTTACCAGTCATTGACATGTGGGTTTGTTTACTATGACCCTTGAGAGGTCCTGTGTCTGGGCTGCCCGTCCACCTTGGAGTTGCAGAGATGGAAGGCCAACGTCTCTGTAGAGATTAGATCACCTTGATTTTCCCCAGCGACAGATCCGCACACACCTCGTCCTACCTCCCGCACCAATAGAAAGCCAAGAAAGAAGGGCAAGGCTTGGAGAGAGACTCCATATGGCTACTGCTCCTGCAGAAGATCTAAGTCCAGTTCCCAGCACCCACAAGGGTGGCTCACAACTACCTGTACCTCCAGCTGTAAGTGATCTGACTCCCTCTACTGACCTCGGAGGGCACCTGCACTCCCATGGGCAAACCCACTCTAACATATAATTAAAAAATAAATACATACATAAATAAAATCTTTAAAGAAATAAAGAATATGCTTCCATGAACAGGCTCAGTTTCCCGGCATATTTTCCCTACAGAGAAAGATCCATCTCTTAGCCCAGGCCAACACAAATGGGTGCGGAATTAATTAATTCTGCACAGCCTTCTCGGCTGCAGCTAGCTAGGAAGCCACAGGCTAAGGTGCCTGCCTTTTCTGCAAGATTAAATTAGCTAGGAAAAGACTCCTTGGTATCATCGTCATATTCAGGCAAGCCCTCTATCACCTAGCTACACTCTCAACCAGGCCGTTTTATAAAGAAAGAAACTGTCATCCATCAGGTAACATGTCTCAAGAATTTGCAATCTGGCTTGATACTCCGGGTGGCCCCACTACTCCTTATCCTCGGGGAAACTAAAACACGGTATCCCCAAGTTTCCACCTACAAGCTTTGAGAAAACGTCATAAATTAAATGCCCACGATTCTGTGGCAAACGGGCGGTACAAGTAGGCTCGTATTGACTCCTGGAGCCCACAGGATGCTACAGAATGAATGAATGGACCATCTCCGTCCCACACACCCTGGTCCTCCCTAGGAGGCTGCACGGTCCCCTACAGGACGCTTGGGCGCGAGCGGCGTCCCCAGACCGGACGGCGAAGCGCCTTTGCCGCCAGGCTCGCGGGGTCACAGGGTAGCAGCGGCCGAGCATAGCCTTCATTTACCTGCCAGACACTGCAGCAATCACGGCCTCTGCTAATCCAGCCTAGCCAATCCCTGGCGTGGCCAGGGCGCCACGTGACTTCCGGCCGGCAGTGCCACCGAGAACGCGATCGGCGCTAGGACCCTCGCACAGGGAGGCCTGCCTCTAGTCCGAGAAGGTCCGCCCCTGCCGTCACATTCAGCCAATGGAGAGCCGAGGCTCCGGCAGAACCAATGGCGAGGAGGCATGGAGGGGCCTGGCGTTGGCGTCACTAGGTGGGGCGGGGCGTGCGGCGGATCACGTGGCCGGCGCGTTGGCTGCGGGAAGGTAAAGCAGTGCTGGAAGCGGATTGCAGGGGTCATGCCGTGGGGTCGTCTCTGCGCCTGCGCCCTACCGGGATGCTGAACCTGGCGGCGCTGCTGTGGCGCCGGCTCCTGCGCAAGCGCTGGGTGCTCGCCCTGGTTTTCGGCCTCTCGCTAGTTTACTTCCTCAGCAGCACCTTCAAGCAGGTAAGCGACTTCTCTCAGGACCTCCGTCCTTCCGATCCTGCTTCCTCCCTCAAATACTATGTCCTTCTCCCCATCGTTCTTCTAATCCTCCTCCTGAGCACTCCTCCCTCTGATCTAAAATCCTCCTTCAGCCCTTCTCCCTCCGTTGCGTTGTCCTCTGCCCTTCTCCTTTCCCCTCCCTAGCTCCCTCCGTCCTCCACTCCTCCTCCTCCCTCGAATTCTCTGCCTCCCCATCCTCTGTTCTCCTCTGCCCTCCAACTTCTTACCTCTCACCCTCTGTTCTTTTCCAACCCCTCCTCCTCGATCCTCACAGTTCCTCCCTCCCTTGATCCTCTGTCCTCTGATGCTCCTCTCTCCTGTCCTCCACCTTGTAGTCCTCCTGATATGCAGCCATTCAGTCCTCCCTCTGAGCCTCTGAGCCTCTTCCTTTCAGTCCCTGGATCATCCATTGGTCCTCCCTTCAGCTCTCTAGTCCTCAACCCTCTAGTCCTCCTCCCTCCGATTGTGTGATCCTCCTCCTGTCCTCCTGTGAATCGAAGGCCGCTAAGGGAGGCGTCCCACTGCAGGTCTTTGTACCGCCCCTCTTCCGTGCGGGATCTGGCTGGTAGGCCAGGCAGAATGGGGTCCTCAGACCTGTGCACCCTCCTCTGTCGCGTTCCCTGCTCCGCACAGCCCCAGGCTTCTTGATGGGCAGGAGGTAGAGATGGCTAGACCAGGAAGTGGCCAAGGGCTCTCTGTGCCTGTTCACCTGGGTGACCCAACAGGCTCCCTTTTGGGGCACAACTTGGTTGGGTAGATTTTTATTTTTTATTTTTTTATTTTTAATTTCTCAACCTCCCTTTCCCGTTTTTGTCCTGGTAGAGAGTTCCTAGAGACCTGGCATCCCACTGTAGTAGGAACCAGTTCCCAGCATTTGCCTAGAAACCACACTTGACTGAGTTGCATTTTGTAGGTCACCTGCCCCTCCGCCTTTCCTGCTCCTGTGCACCCTTTGATCCTGTTTTGCTGTTCTTACGAATATAGTTCTCTGGTTTTCTGTATCACAGTCCGTCTTTTGAATGGTTGCGCTTTTGCACAGTCACTCCTGGGGGGGGGGGGGGGCGTGTCTTACTCTCTTGCCCAGATTTGACCTTGTAATCCTGTCTGGCTTTTCCACATTCTGATAATTGTCTCCATTGGCTTTTCCACATTCTGATAATTGTCTCCATTGCACCCACCTGGAACAGAACTCTTGCCTTTCTTCTGCCAGATTTATACCTCTCCAGTCCTCAGCATCTCACTAAGTGACGTATGTCATCACTGTTGACACAGTTAGGCTGTTCATTTCTTCCTGTCACTTCAGGCCCCAAAACTCTGGCTCTACAGTGCCCTATTAGGAGGTGTGACCTTGTTGGAGTAGGTGTGTCACTGTGGATGTGGGCTTAAGACCCTCACCCTAGCTGCCTGGGAGTCAGTCTTCCACTAGCAGCCTTCAGATGGAGATGTAGAACTCTCAGCTCTGCCTGCATCATGCCTGTCTGGATGCTGCCATGTTCCTGCCTTGATGATAACGGACTGAACCTCTGAACCTGTAAGCCAGCCCATTTAAATGTAGTCCTTATAAGAGTTGCCTTGGTCATGGTGTCTGTTCACAGCAGTCAAATGCTAACTAAGACACTCATTTGCTTGAATTACTTCTGATGCCCCTTCAGGGGCAAGACACTGTCATCTGTTCCTGGTCTGGTGCCATAGCCTGGCCAGTCTGCTTCTGCTTTGTCTCACAGTCTGTCCTAGGGTGATAGTCTAGACAAGGTTTCCACCACAGGCCTTCTCAAATGTGTAATCCTTTGCTCAAGCGAAGAGGGTTCATCTACATTGTCTGTGTGACTGAACTACTTTTGGTCAGTTTGGATCTAACTGACCGTAGTCAGTGATTCGGGCCATGCCCGGTGCCTCACTGTAGTGCAGTCTCCTGCCCATCGTCTGGCCACTTGCCTTTGTGGTAATTACTAACCCAGCATTTCTTGTTTGTGTGTTTGCTTGTGTGTTACCTCACCCTCTTGCTCAACATAATGTTTTTTGAGAACAGAGACCTGCGTGTTTTGTTCGGACACTGCAGCCTTCATCTCTACCCGAACCCCCCAGGCACTTCCTGGGGACCAGATGTTGACCAGATGTTGGTTCAAGTGAGTCTTGGTTGAGGTGACGACTTTTCTCCTAAGCCAGGTTATTTATTTATTTATTTATTTTTAATAATCTTGGCTCAGATGTTGTCTACGTAGTGACCTTTCCTACAGCCCCATTTTAATTACTTGACACGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTTCCTGTCTGTCTGTGGTAGGGTAGGTATAAGTATCTCCCTAGCTATTCTGGAACTCACTCTGTAGACCAGGCTGGTCTTGAACCCACAGATCTGCCTGAGTGCTGGGACTGAGGGCATGCACCACCCCTGGCTGCCTTGCTTGCATCTATTTATTTATTTATTTATTTTTGTTTTTTTTTGCTTGCGATTTAAGACTATACAAGGGTCATTATGAAACCTCTAGTTTGTTTGTTTGTTTATTTGTTTGTTCTGTTTCCTCTGAAGCCAGAGTACTGATTATCTTTGCCATCCTGTGCTGTCCCCTTGTCATGATGGGGCTTTTGGCCAGTAGATGACTAAACACTCTGGACATGTGGAGAACCTGTGTGAGATCAAGTTCCATGAGGGTGAGAAGAACTGTGTGCTGAGTGTGGGCAACTGAAACCCCACCATGGACTCTGGTGGCCTGAACACCACACATGCACTCTGGCAGCTGGGAGGTATCAGACGGGGATGCCAACTGATTCCACGTGTGGTGGTGCCCCCTGGCCTGCTTCATTCTCGCATAGTGGAGAATACACCCTGGTCCCTTCAGCCCCTTTCAAGGCTACCAGTCCATTCATGGGGACTCCACCCCATGATCTCACAGAATTCTGGTTACATAGGAGACACCCCACTTCTCAGTCTGTGTTTTCACTGCTCTGCTAATGTCATCTTGGGTGTGAAGGGTTTAACATTGATTTTGGGATGGGGGTAGGCAGTCAGTTCACCACTGGATAGCTACATGCAACTCTGTTGGTCCTGGAACTGTTGCACGTCTACCTGGCTTTCACCTGTGCAGTGGAGGTCTTAGAAGTGGTTGGTGGGGTGTACTATCAGGTTGGGATGTGATACCTTTAAGAGAGTGGCTGATACCTCAGCCAGCCTTGTGCTCTTGCCCTACCTAGGGTACAGAGAATAGCCCTCACCTGTGTTTTAACACTGATTCTTGGAGCACTCTCTCCTTTTGGCTCTTGGAGCACTCCCCTGTGAAGCTCTTAGAGCAATTTGCTGTTAACTCTTGGAGCACTCTCCTGTGCAGCTCTTGGAGCACTCTCTTGTTGGCTCTTGGAGCACCCACCTGTGCTGTTTTTAGAGCACTCCCTTATGAAGGTCTTGGAGCACTCTCCTGTTGGCTCTTGGAGCACTCTCCTGTGCTGTTTTTGGAGCACTCCCCTATGAGGCTCTTGGAGCACTCTCCTGATGAGTCTTAGAGAACTCTCCTGATGGTTCTTGGAGCACCCTCCTGTGTGGCTCTTGGAGCACCCTCCTGATGACTCTTAGAGTACTCTCCTGATGGTTCTTAGAGCACTCTCCTGATGGTTCTTGGAGCACTCTCCTGTGTGGCTCTTGGGGCATCCCTATAGACTGTTAGGGTTGCTTTGCCTAAGACTCATTTGTGGCTGTCATGTCTTCCATTCTCTGGTTTCAGGCTGTTTTCTACCTGTGTTGCTTGACCTTCTGTTTGTGAAGTGGAGCCGCAGTCTGGATGTCACTCATCTGTCTCATCTTCATGTCCTGTGGCCAAACTACTTCGCTGTGGAGCGAAGTCCTTTTCTTAGGACTTGGGTGTGGTTAGGGATTAGGCTTCTTTGAGGAGGTGTGGATTGTGAATGTGATGCAGACCAGCTGGGATTTTCAAACTGAGGTTCTTCTTTTGTGACAAGGTGACAGACGTAAGCCAGCATCCCGAGTGAAGTGTTTTTAAAGTTGGGAATGGAAGCACTCACCTTTGGTCCCAGCACTTGGGAGGTTGAGGCATGCAGATCGCTATGAGTCTACATAGTGACTACATAGTGACTACATAGTAATAGATCCCAGTCTGCAGGGAGAGTTCCTTAAAGATGAGGAGGTGGGAGAGGGGAGGAGTCTGGTCTAGATACATCTGTAGTTCCCACCCTGTAGTGCTGTCTTTGCTCACCGAGGGACACCACTGGTGGCACAGACAAGAAATGTGTATTGTGTTAAGCTCTTGGGGCTTTTCAGAGTAACTTAGCCAGTTAGTTTGCACACCCCAAGAGAGCCCGCTTCAAGGAGTCTGTGAGGTCCGAGTTAGTTTTGAAAGAACACTTAATAAACCATGTTGGCTGTGTTCAGCTGTGGTGACACTTGTCCTAGTAGTAACCGGTCATGGTAGAAAGTGCTTTCAGCACACTAGGGCATGAGCCATTAGCATTTGCCTTAGAGTAGATTCTTTCCCTGTTGCCTGGACAGATGCAGTTGCTGGCTCACTGAGGAGGAGGCTGGGAAAATGACCGATTGAAAGCTTTCAACTACTCTGCTGAGGTGGGAGGTAGGCAGCCTGTGCTGCCTTCTGCATACTGAACGCTAGCTGACACATGAGTTACCAGTTAAACTAGTCAGCCCCACCCCTCCCCACATCTCACCTAGAGGAATGAGTGGTCGGTCAATGGTTTGTTTATAAGACTTGGGAGAGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCAGATGGTGGCTCACAACTATCTTTAACGGGATCTGATGCCCTCTTCTGGTATGTCTAAATCCAGCAACATTGTACTCATATACATAAAATAAAAAATAAAATAAAAAAAAAATAAAAAGACTTGGGAGAGAGCCTGCTCCCTCAGAGACAGTTGATGTTCTCCACTGCCAGACATAAAATGGAATTCAAGAGAAAAATCAGACTTTTGGAGACTGTGTTTCCTCCCCTGAGTTGTTAACTTCCATTAGCTGTGACAAGAACAAGCGTGGTATATAATGTGACAGAAGTGTGTCACCATTTGGCAGATCTGCGAAGATCTCTTCACAGAGCAGGACATTTCTGAGTGCTTGTGAGTGGGTAGGCCATGCTCAGAGCCCATGTGCACACGCTGACCTACAGTTCACCTTCCAAAGCCATACACAGCTCACCCGAGTGCATATCCCAGTGGCTTTAGTAAGGTCACAGTGAGGTAACTGTTACCACAGTCACAATTGAGGACACCTTCATGGACTTGCTCTCCCTCTCAGACCTAAACAGTCTCCAGTCAACTTCCTGTCTGAAAATGTATTGCATTCTTATTTATTTATGTGTGTGTGTTTGTGTAGTGTTGTGGGAAAGGAAAGAGTGCATAAGGTCAGGGGACAGTTTGGGGGAGTCCTTCCTTTCCATCATGTTGGTCCCCCAGGATTGAATTTAGGTTGTCCGGCCTGGTGGCAAGCACCTTTCACCCACTAAGCCCACTGAGTTTTGAGAATCCATCTGTAGGCACGAGCTCTCACTGTGGGTCCTCACTGGCTTGGACCAGTCGTTCTTGCACAGGTTCTTCTCTGTGCTCTGCCATTGTGAGTCAGTCCCATCCTGACTTGGTTTCTTGCTTGGCTGACTGCCACCTGCCACCTGCAGCTGGGTGCCCGGTGGGTCTCACTTGGCCTGGCCTTCCCCTCAAATCTCTGACGTGGAGGTGGGCTAGGAAGAAAAGATGCTCATCCCAGATAGAGAGGAGGCTGTGGTGCGCACAAGTGTCGCCCCCCCCTCCCTCCCTCCCCCCCCCCCCCCCGTCCTTGGCTCTGCATCCCTGAGACTTCTCAAGCTTGTACAACGGTCTCTGCCCAGACAGCTGCTGACCCCCGTTGCCTTGGATCCTGGGATGCCTCTTTTCTTTGGTTTTTATCCTAATTAATGCTTTAGGGGATAATTTAAATTGCTGGAGAGATGGGAGGGCAGAGAGGATGCCATCAGGGGAGAGTGTTGGGGATGGTGAGTGGCGCCGAGTGGCCTATGCACGGGGGATGGAGGCGGAGCGGGCACTGAAGTCTACTCCCTGGGTGCCTGCTGCAGGAGTGGGAGGGAGGGAGGGCAGGTCAGTGAGTTCTGCTGGGAAGATGCAGATTGTCTAAGGTTGTTGTCTTTGTTGCTTTTCTATCTCTCTGATTATACACCGTGACCTGGGCAGCTTAGAGTTTGGTTGGCTGGGGCGCTAACTTGGGCTTTTGAAACCTCAGAGCCCCACCCCCAGTGATACTTCTCCTCCTCCAAAGCCACACCTCCTAATCCTTCCGAAAAAATTCCACCAACTGGGGACCAAGTATATATGTATTCAAATACTTGAACCTGGGGCGCGGGGGGGGGGGGGCATTCTTAATCAAACCACCATATAGGCATTGTATAAGACGTGATGAGTGTAGAGAGCTGCCCCCTGTGTTGTTCCCCTTACCCAACCCCAATTTTAAATTGGGTACCAACAAAGACTTGAGCTCTGCAACATTTTCGAGGCATGGCATGGGCACGGGGTCACCCACAGGAAGAGCTACTGGGACATTTGGGTGTCATTTCTTCCTTCTGTTTCATCTGAATGGTTTTGCTTCTCTCACTTGAGGTGTGCTGTTGTTCCCCCCATTCCCCCCCCCCCAATTAATGTACAAGTGTTTCCCCATCTTTACAAACTCTCTGGGGGAAGGGGAGGGTGCTGCAGAGGCGGCGCTGTGGCTAAGAGTGCTGGCTGCTTTTGCAGAGGACCTGGGTTCTGTTCCCAGCCAGCAGCACCTGCTAACTCTAGTTCCATGGGATTTAATGCCTTCTTCTGGCTTCTGTATGCAAACCCTCACCCCCACATACACACAATAATAAAAGTAAATCTCGTTTTTAAGAGTTCTTTATTGGGGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAGGGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACACCCATCTGTAATGGGGTCTGATGCCCTCTTCTGGTGTGTCTGAAGACAGCTACACTGCACTTATATACATAAAATAAATTAAAAACATCTTTTTAAAAAATGATTATTATTTTATGAATGTGAGTACACTGTCACTGTCACTGTCTTCCATGGGACATGTCTCTCATTTGACTGTTATGATTACCCATAAAAACTAACACAGCCCACTTCCTAATTAACCTCCTGAAAATTTGAGGAGAGGCTATGTCCATTAGCTATGCAGGCTACACAACTGTTTACAGAGAACATTCTCTTGTGCTCCCCCCACTCCGGATCCCATCTTTCCTCTGCAGGATCTGTGGACAGCTAACACCTCGAGGGTTGGGTGGATCTGATTCACACGGAAGCCTTTGTCAGCAGCCCATTGGAGCACTTGTCTAAAGTGTCCATGGCACTCTGTGAAAATCCCCTTTGAATGGATATTTTTTCCTTGTTGTTGAGACAAGGTTTCTCACTGTAGCCCTGGCTGTCCTGAATCATCTCTGTGTAGATAGACCAGGCTGGCTTTGAATCTGCAGAGATTCACCTGCCTCTGCCTCTTTGAGTACTGGATTAAATATTCGTACTGCCACGCCCAGCTTTGAATGGGTCTGGAATGAAGAAAATCATATACTTGGTGAAGTGCTCAAAGATAAGACAGCCCAGAGGGAACTCAGTAGGGAAGTCCAGTAACCCACTGGGTGGAAGAAGATCTCCTGTCCCTTCCACCCTGCCTCCAAAAGTGCACCACAGCCACGATTTAGCCACGGTAGAGGTTTCGAATATTCATGTTTGACACGTTCAGGTGCTGGAGAACTGCCCTCCTGTTCACCAGGAACAGGGTCCATGGTTGAGGTCCTTCTGGGGTCCATTGCTCCAGGCTTCAGCTAAGGTCTGCTCCTGATCCACAGGCTGCGCCTAAGGGGGCCCCTGTCTCCCACGGGTTCTTGCTCTCAGCCCTTCTCTTTCTGTAACTGAGGTAACAGCTACCTGTCCTGTCCTGTGCCACCTAGCCATAGCCTTTAAAAGAACCAAAAAAGGAAAGGCAGGCCACAGGAAAGCCATCTTGAAGAGACTGTTTTTACATTGAGCAAACTGAAAAAGGGGAACCTAGACCTGCCGGGCTTGTGGTAGGAGAGAGGCCAGCTGATGATACCTCAGAGGGTTCATCAAACTTTGGGGAGGCTAGGCACTGTCTGGCACTTCCATCTGCAAGTGATTGACAGGACTTTCTCACACCCCCACACCGAACTGCAGGGTCATCCTGCGTGCAGAACCAGGGGGCTGTTGCTGGCCTGTGGAGGCTGTGTTCTTAATGATGGCACATTGACATGGTGGATCCATGGCAATGTGATTATCTAGATAACGTGGGTCCTTGGAAATGCTTTACTATCCACTTAGTGTTGAGATCAGCCGATGGTGACTCCAGTATGCCCTGGGTCAGAGGTCACAGGTCAGAGGTGAACATGTGGGGAGGTGTCATGAGAACTTAGCTCTGATGATAGTGTGTCCCTGCTAGTGTTTAGTCAGACATTTCCGTGGTGTTGGGTTTCTAGGTTTTTGTTGTTGTTTTGGGGTTTTTGTTTTGTTTATGTTTTTCCTACCATTGAAACAGAGAGCTATGGGAAACCTCCGTGTATGGTTCCTGTGGGATGGCACTCAGTAAAGTTTCCTAGAGCAAGGTTTATGCATATTCAACTTCAACAACTTCCCAGATTGCCTTCCCACAGTAACTACAAAGATCCTGTCTGTTTCCCTGAATTGTCTGTGTTGCCATCCGATGCAATGCGCAGGGTCTCCATAGTTACTGAGCAACATTATTGAGGCTTAAAAAACACCAGCCATGGGGATAGCAGGCGTGGTGGTGCACACCATAATCCCGTAGTCCCAGCAGCACCTGGCAGGCTGTTGCAGGAGGACCTGCGGCCATTTGGGGTTGCAGAGGGTGACCCTGCCTTAAAACCTCCAAAACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCCACTGCTCTTCTGAAGGTCCGAAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCACCCGTAATGAGATCTGATGCCCTCTTCTGGTGCGTCTGAAGACAGCTACAGCTAAGTGTACTTAAATATAATAAATAAATAAATTAATTAATTAATTAGAAAAAAAACAAAAAACAAAAAAAAAACCTCCAAAACCAACAGGCCATGGTGCCTCACACCTATAATCACAGCATTTAGGAGGCCGAGGCAGGAGCATTGCCATGAGTTTCAGGCAGGCCTGAACTACAGGATGAGAAGCGCAACAGGAGAGGGCCTTTTAGAGGTCTTTTGTTACACATTCATGGCTAGAAGCTTCCAAAGATGTGACCACCTTGTGCTTCTCTATCTTTCCAGCAGCTGGGCTTCTCCTCCTAGCTCAATGGTGTGGATCCTGTAGGGATCCAGGCTCTCACCCAGGGGCCACTCCTCTCCTGGCTCAGCAAGGCAAAGGGCCGTGTAGGCAGAAAGGCATCTGAAAGTAATCATAAGGGTGACTGCTGTGTGCTGTCACACAAAGGATGAGCCTTGCAGGTTGTCAGGCTTCGTCTGGTCCTGAAGTCATCCCCAGTCCCATTTAGAAGTTTTGTATG
D3_15 118706060 118721110 15051 CACCGCCTACCAGGCACAGGCATGGGGTTCCTCATCTTGTCTCTCTAATTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTATGTGTATGTCTGTGTGTGATATGTGTGTCTGTGTGTATATGTGTGTCTGTGTGTGATATGTGTGTCTTGTGTATATGTGTGTCTGTGTGTGTCTCTGTGTGTGTGTAGTCTGTGTATATGTGTGTGTATGTGTGTGTCTCTCTGTGTCTGTGTTTGTGTGTGTATGTGTGTGTGTGTCTGTGTGTGTTTGTGTGTGGTCTCTATGTATATATGTATGTCTGTCTGTCTGTGTCTATGTGTTTATGTGTCTGTGTGTGTGTGTGTCTGTGTATGTCTGTGTTTCTGTGTTTGTGTGTGTGTGTGTGTCTGTTACACCATGTCATGTATGTGGCAGTCAGAGGACAACAGGCTGTTTTAGTGTTAGATGGCAGTTTTTATCATAGCTATAGCTACAAATTGTTAATTTAGGTCAGACAAAGACTCACACTCCTTCAGCTTCTTAATCAATAAAAATCCCACAAGGGCAGCAGGTTTTACTTTGCCATTATAAATATGGGGGAAGTGGTTTAATAAAGAAAACTTGCAACTGTTGATGCTGTTTAGCAACGAGATGCCACCGTGTCGCCAGGGTAACAGCACTGTTAAACAAGATAGCCAATTAGAGTCACAAACTTTTCAAAAGCTGGAAGTTAATTTCCCAGGCAAGACCAACACATTTGCCTGCCCTGGTCTCCCAGGACTTGGTAAGAATAACATCCTTAGAGCTGGTACGCACCACAGGCTTTCTCTGTGGGGCCCTGGCTGTACTGGGGCTCGCTCTGTCGACCAGGCTGACCTCGAACTCAGAGAGATATGCCCATCTCTCCTGGGCGCTGGGATTCAAGGCATGAGCCACCACACCGGCTGGTGTATATGTGTTTTGAAACATCATGATGACCCAGTTCTATGTATGATGTGTGTGTGCGTGTGTGTGTGTGTTTGTGTGTGTGTATCAGATAAGAAAGGTTGGAGAAATGGGTGTGTGGCCAAGAGCACTGACTGCTCTTTCCAGAGGACCTGGTTTTGAGTCCCGGCATCCAGGACAGGCAGCTCCCTCTTCTGGCCTCTGCAGGAATCTGCACTCACATGTACTCACACATCCAATTAAACATAAGGATGACTTCAGAAAGTAAAAGGTAATTTTTGCATGACACATGGGGAGAGAGGCCTGGGTGAGCTTGCTCTCTCTTCCCAGAGATTAGACCTGGTCATGGAGAGGTGAGTACCAGAGTTCAAAGCCGAGATCTGGGAGTTGGTAACGGTAGGACCAGGCCTGAGATGTCAGCTTCCAGCTTCCCAAAGGAAAGGACAGTGAGTGTCAGTGGGTGATATCGGGGTGTTAGTGTCAGCTGGTGTTATTGGGGTGCACAGTATTCTTGACACAGACGGAGTTGCTGTGTTGCTCTCATCCAGGGTAGAATCCTGAGGAACGCACGGGCTCTCTGGGAATTCTGTTTAGAAGCCTTGTCAACAGCGTGAGTCAGGGCATCTGGGAAGGAAGTGTTTACCAGGACATCAGAATGTTCATCACTACCCGTGAGATGCAGCAGCAGCAGCAGCAGCAGCAGAAACAACAGACGCCAGCCTTTCCCTGCACTGGGAGAGGTTGGCGTCTCCCCTGGTCTCCCAAGTATTGAGGGCCCTCTGGTGTCCCCGATCGCCAGTGTTCTGCCAGCTCCCACACAGACCTTGTTTTCCAGAGCAGCTCTAAAAAACAAGTCATCCTCCGCCAGCCTTGTTCTCTTGAGGACAGAGCTGGGGTGCTCAGACTGGCTGGTTGGGAACAGCTGCTGCACAGCTGCGGGCCCCTGCACACACCCGAGGGGCCCCCCATATTCACCAGAAGGCCTCTGCACACACCTTAGGGTCTACACACACACATACACCTGAGAGCCTCTGCACACACCCGAGGGGCCCCCCATTCTTAACAGAAGGCCCCTGCACACACCTTAGGGTACATACACACACACCAGAGGGCTCCTGCATACACCCGAGGGTCCATACACACCTGATCCCCCCCCCCCCCCGCCCCCGCCGCACCTCCCCATACACCTGAGGCTCTCTCACATGCACACATACCTGAGGACCTAAATGGCCACTGGGGGCGTGTTCGAGGTTGGGTGGTGGCCCTCATAGACTTACATATTTTTCTGTCCTGTTCCTCACAGTGCAGGGAACAGTCTGCCTAGAAGCCCTGAGCAGTTAAGTAGTCTCAGTGAGGGAGCCTGACTGACATGGCGCTCGATGTGCACATATCTACGTGATGCAGTTTCACAGTTGTGGAGCGAATTAGGTTTTTCTCTCTTTTGAGGATTTGAGAACACCCCTAATTCTAGGGGGGTGCTTGTGGTTGTGTCTCCCCCTGTTGGTATGCAAACACCGGAAGCAGAGTCACGAAGGTTGACTGTGGCCTCCTGTTTTAGCAGCCCCTTCAAATGACCACAACAGCCACGAGCCATCAAAAGCCACATGTTTGCTTTTTAAATCCGCAAGGCACTTGTTTTTGTGAATGGGTGCCTCGTTTCCTGTCTTGGACTGTTTTGTTAAAGCCCAGGTGAAAGGCTGCGTGCTTCCATTAGCATCCCAGCTGCACAGACAGGGCATGGTCTGGCAAGAAGCTCACACTCAAGCAGTAGACTGGAAAGGGGCGGAGCGTGGTAACAACTCAGATCTATCGATGACTGAGGGGTCCAACTTCCCGTCATCCTCTGCCCAGGTGAAGCCAGCCCTAGACACGTTTCCATGTCTGGTCCTGATGCTCAGGGTGCCCTGGCTCCTGCCTGCCTTCTTCCTGTGCAGTTCTTCATCCTCAGAGGGAACACAGGTGGCTAGCTTCCCGCTGAGCTCTGCCTCTGGTTTCAGGCTATGTCTGCGAGAGGAAGGACCTGCTGGCGAATGGCTGTTGCGACGTCAGCGTCCCCAGCACAAAGCAGTACTGCTGTGATGGGTGCCTGGCCAATGGCTGCTGTGAAGCCTACGAGTACTGCGTCTCCTGCTGCCTGCAGCCCAGCAAGGTACGGGTCCCATCCCTTGTGTTCTGTGCATTTTAGGGGGGTAGCTAGGCATCTCCTGCAGTGTTCTGATGGTGCAGTGGCCTCTCCCGAGGGAGGAGCTGGTCTCCCTGCCTCACAGTTCCTTCTGCAGAGAGGACAATGCTATTGCTAACTAGGTATCTGGTGAATGAGAGAGACCTGGCTCCTTGCCCTCCCTGTGGGCCAGCTCGGCCAGCACTTACTAATACCAAATGCCTTGTTTCCATAGCAACTTCTCCTGGAGCGCTTCCTCAACCGGGCAGCTGTGGCCTTCCAGAACCTCTTCATGGCAGTTGAAGACCACTTCGAACTGTGCTTGGCTAAATGCAGGACCTCCTCCCAGGTCAGAAGTGGTGCTGGGGATGGAAGAGAGAGACAGAGCTGGAGAACCCTAGCTGACCGATCTGGGTAGAGCCAGGGAGGAAAGGGGCTTCATTGACGTTCTGCTGTGTTAGGGGGTGAGAGAGTTAGATCCAGTACCAGCCATTGAGAGGAGGCAGTAGTACTTGCTCTAGGGCAGAGTTGTGGTTCGAATTAGGATCCAAGGGGATAGGAGAGTCAGGCACATGGATATATCTGAGGCTTCTAGGGTGGGCACACACTGCCTGCCATTGACTGTCTGTTCTCATAGCAGGGAGTGCGCTGGAGGGCCTCTGCCACCATCACCAACCCCATCTCTGTTTATCTTCCCTTTTCCGTTTATTCTGGGCTCAGAGCCACAGACACAAGTCAGAGGACAACTTGTGAGAGTCTGTTCTCTGTCTACACCAATGTATATCTCGAGGACCAAGCATTGGCAGCCAGCACCTTTCAGGCTAAGCCATCTTGCCTACCCAGTCTTTCTGGCTAAGCGCTTGTCTCTGCCCTTTCTCAGACAACCACACGTCTCTTACACAAGCACACCCTTCTAGGATACACTCCGGCTTTGTGCTCGTTAATCCCCCTTCCCATCTCTGGGACAGGATAGAGGGAGCGGAGAGAATGTGAAATTCCACTGGTCTCCATGAGGAGGGGGCTTCTCAGGTGAGTGGGCGGGAAAGGAAGTCGGGTCACTTGCTCTTCCTGACCCACCCTGGCAAAGGGAACTGACTAGGGAGGTTCCTGCCGCTGTCCAAGCCGCAAGTCACTTCCTCTTGTGCAAATAGAGATAGTTCTCAATGGCCACTTGGTTCGAGTGCCCAGAGCCCAGGCTGCGAATCTCTTAGGTGGAATTAATATGGATGTATGGCTTTGGTAGGGGATAATTAATGAGCTCTAGACCAGCCAAAGCCTCAAATTGTTTGTATGAGAAGAAATTGCTGGGGGCGGGGGGATCTGTCAAGAGGGGTGGGGACGGGACATCATCTGGGCTCAACCTCACTTGCTGTGCCCAGCCAGGGCACTCTTGGGGTGGATGCTTGACTTGTGCACAGGCCCTTAGGGCCTGACTGCATTTTGACGTGTCAGGACTCCGCCAGGGACTGGGTCTGTCCAACCACAGGGTCAGCGGCCGATCTCGCTGCTCCACCGTATCTCCTTGTCTGTTTCAGATTGATTTTGTTTGTCTGTTCTCTTCATATTTACTATTGTGGCCGGGACACGCCACCATGTGCACGGGAGGTCAGAGAACAGCAGCTCGCTTCTCGTTCTCCTCTGTGGGTCCTGGAGATGGAGCTGAGGCTCTCAGACTTGGGAGCAAGCACTGTGTTAGCCCTGGCCATCCCATCTGCTCTTTTCGGATGGATGGAGCAGTCTCAGAATCTGCAGCCCGCAGTGCGCCTGTTGAGAAGCTCCCTGGTGGGCAGAAGAAGAAATGGCACAGGGATGTCCTATCTATTTGGTGGCACTCAGAGCCAACAATATCCTACGTCATGCTAGAATAGTACCTTCTAGGCATGCTAGAGGTCTGGCAGGGACTGTGGCTGTCACTGCAGCTGACCCTCTCTAACACACCCCTGTCTTCCTTCGCCAACAGAGTGTGCAACACGAGAACACGTACCGTGACCCCATAGCCAAGTACTGCTATGGAGAGAGCCCGCCTGAACTCTTCCCGGCGTGAGGCCTGACGGAGATACACACCCAGAACCAGGCTGGTCCACTGGAGTAAAGAAGGAGAAAGCCACACAAGGGTCCTTGTGGCTTTGGGTGTCTCCAGCTCCCTCACCGACAGCTGGAGCTGATGGGCGGAGCTGTTCTGTAGAGCAGGCTGGATGAGGTCCTGGCACCTTGCAGGCCCCTGGGACTGCTGGTCAGAGGCCAGGCACTTGCTTCTGTTTTGCTCCTACCTTGTAGTTATTTATTGACTTTTCAGAGTGGTGGAAGGTACGGTCATTTTTTAATTTTTTTGGTGCAGGACACCTCTGCCTGTCCAGATGAATACATTGGAGGAGGTGACCGTTGCTCTCATTTCCTGAGCGTCGCATGGATGTGCGTGCATGCTCCTTCACCGGATGAAAGTTAGTCTCTGGGAGTGCGGGTGTACAGACTCAGTTGTGGTTCTGTTTTAACATCTGACCAGCAGGCCCTTGTATCCATACCCCAACATTCAGGGGTATCTGAAGCCTCAGGGAGGTGCTGGTCCCCACTGAGCCTTCATAGAGCTGCCTCAGTGTGCTCATATACTGAAATGGTAGACAGGAAGCCTGAATCCCAGGCTGACTCTCCCAGTGGAGTTTGCTGTGTCTCCTTGAAACCCCTCATCCTCAGGGCAGGGGAGCCCTTCCCTTGTCTGTCACAGAGGCTAGGGGGACTGCGACTGTGTGCATGCACAGTGGCCATTCATTCTTGGCTGCTCTCCACGCCCGGTGGCATTTATAAAGAAAAGCCCCAGCTCTTAGTCCAATCTGTCGCAAGTTTCACATCCACCATCAGTGGGAGACGAATGTGGTAGCCACAGTCTAGGGAGCGTCCCAAAGGCAGGTCCATGTCCAGTGCTCACAGGACAGTTGTTACCAGCGACTGAAACGGAGGTTTGAAGCCTAAGGTCAAGACACCTCCTCCCGTACTCTCCTGTCCTGTTTACAGGCCAGCCTTTAGCACACACTTCCTCCCAAGACCTTTGACCCACCCTTCCAGTGGACCACGGTGTTTTGTGGTTTTGGTTCAGGTTGACCTGGGATGGACGGGCCGCGAGTCTCAAGATTTCCTATCGGGGCCACATGGAACACAGCTCTTTGTACCGTCAGGATGGAATTTCCCTCTGCTCCCTCCTGCTTCCTCTTGCTGTTCACATCGGATTTGTATTCTCTCAGCTCTACAGAAGGACAAACTGACTGTCAGTTGCTCCCTTGTTCTGTCTTGCCATGCTGGGATCGAACCCAGGACTTTGTGTGGGATTGGCAAACTTGCTGTTCCTGAGCCACGGCCCTGGCCAGGGAGTTGATTTTTAGTGTTAGATGCCAGTGATTCTCTTCTGTTTGCTCTGGACTTCACGGTGTGTTGACTCAAACTTGATAGAAGAACTGGGTGATCCTGCACGTTCTGGCACATCCTGGAATGTTCTGGCACGTTCTGACATGTGCTATATGCGTTTGACACCAGCCTTAAACTCCTGACTCTCCTGCCCTGGGAGGAGTCCACCCTTGGTTGCCAGTGGCATTGGTCATATTTAGAGTGTTGACCCAAGTTTTAGTATGAGCAGTCACATCTGTTGCCAGCTCTCCCCGGCTTGGCACCAGAAACGCATGCTGTGTGCGTTTGTGAGTTTATAGTCTGTGCCGTCATGATCAAGAATCCAGTCTGCTCGTGCTTCACTCAATTGATGAGGACATTGCAAGAGACAGGCCCTCCAGAGCCACAGCCAAGACAGAATGAACAGACCCACACAGGAACTTGTCTTGAATCCCACCCCCATGTCCTCTAGTGCAGAATCACCTCCATACCGTCTCCCCCTAAGAGCAGATGGCCTTCCATTATCCCAGACAGGAGAGGGCAGAGTGGTCGTCTATATCCACTCCAAGTCTGTAACACGAATGTCACTTTCAAATTGGCTGTGGCTGTGGAATGTGGTTTTGTGCTTTAATCAAGTAGGGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTTTAGAAATTCTCAGGATTTCAGACAAGTGTGGCACTGGCCAGTGAAGGGCCCCTGCCCACCTTCCTTGAAAGGTTGTGTTTGAGCTCCCTTGATGGGTTCCTAGGATCAGAGGCTGTAGTTAGTTTTGTGTACAAGACTCAAGTAATGTGGCTGGAGCCTGGGACCACTGCTTCTCAACAGCATGACTTTTCATTTAGAAAACGGGGTCACATGTTTACCTGCGAGTGTGCTCTGAGGTGTCCCGAGTGAGAGCCGACCTCCTGAGAGGCCAGTGTTGGCAGCCCCTGGGAAGCTGTAAGGCAGGGATGAGGGGTGGAGGGCTAGAGGGATGGATGGAAGAACATTGGTTGTGGTAATTAGGAACTTAATGCTGAGAGGGCCTTGGCTTTTTAATTAGGGAATTCGGGACCATCTAACCATATAGTGAGGACATGACCGCTCCAAGGTATGGTTGAGGGGATTGTATTGTAGATATGAGGGGGAGAGAGACAGCAAGAGAGAGGCAGCTGGAAGAATCCAGAGGATGGGGAGAAAGGCATGACTGAGTGAGGGGGATCAATTGTGCATGGCCAAAATGGCAGGGCTCTAAAGGGATGAGAAGCTGGGGGAGGGGAAGCTGGAGACTTAAGCTGGACACCCATAGGCAGGCTAGCAAGCACCATAGCTAGTTAGCCTCTTGTCCTGAGGTTGGTTGGTTGGTTTTGGTTTTTTTTTTGGACCTGACACTAATGATAAAATAATCACCTAAAACTAAAGCCACATAGCCCAGAAATAAACACCAGCAACTTTCTCCTTCTTGTCACTTTCCAGGTGAAAACCAACCAACCAACCAAAGGCAGGCTTCCTCCTTGGAAACTGCCTGTGGGTCTGTTATCTGCAGGCCTATGTGACATCCACACAGGACAAGACAAACACATTGGCATGGTGCTTGTATATACAGATGCACGCGCATGCACGCACACACACACACACACACACACACACACACACACACACATTCCTATCTTCTTAGAACAAGCACGGCTGCGTTCTAGAACCCGGGACTATCCGTGACACCAACTACTCATTGAGCCTGCCACCACTAGGTCACAGACTTCCAGCTCATACTGCAGGCAAGTTGGGCGTACTGCTGGGCTGATGGTGAACTCACTAAACTCACACTGTGCAGGGTAACTGCACGGTGCCTTGCGGCAGGAATGTGCTGTTATTTGAGCGTGTCCTGTGGGGATCACAGCGTAATTTAATCAGCACTTTAGCATGGCATCTCTCCAGGGTGGGGAAATAATGAAGTCAGCGCTCAGTGCTGGGAGTTCTCAGTTCAGCCTTGGGCACCGCTGTGACCTAGAGCTGGGCACAGTTACTGCAACACTGCCAGCATCCGAGGTGTGGCTGTCCTAGATGCTGTGGGCCAGGGAGGCTAGCAGCATCGTGGACTCCCAACCTATTAGGGACAAGTGGGTATCTTCCCTATTCCAGCTGTTAAGTAAGTATATCCAGACACTGTCAAATGTCCCCTGAGGGTCAGGGAGGGACTGCCCCTGTGTTACAAACCACTGCCCTAACTCTATTAGAAAGATCGGTTTGTGAACCAATTGCTTGAGTGTCACAGGAGGGAAAGTTGTCGAGAAGAGGCTGGGCTGAGGTTTAAATCCCAGGAGGCTAATGGTGGAAGATAGTTTGAGGCTAGCCTGGGATGCAGAGAAACTGCCAACTCAAAGGCAAGAGAAGGGGGGAGTGGGGAGACAGCGGTATATATATATATATATATATATATATATATATATATATCCAGGCTTCATTTCAAAATGTTCTGTAGTAACCAATAGGAAGATCGAGGCAAAATAATAAATGGTCAATCAAGATGTGGGGTGGGGTTCTGCTCACTGTGAATTAATACACACAAACTGATAGTTGTATTAGTAGATATCAAAAAAGATGTAGAACCAGGCAGGGTGGCACGTGGTTCAATTTCCCTTTAAAGTTTCATAAAATATTTTCTCAGATGTTGGTGATTCAGATGATAATTATGACAATCTGTGTGTGGCTGTAGGCATGTCCCATGCATGCATGGGCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTCCATGGTGCGTGTGCACTTGTGTGTACTGTGTAAGAACAGTGGTCTGGAACCTGTGAACAGCATCCTGACCTCCTCACAGCAGCTTAGGCGTCCATGAACTTGGATAAACTCGTCTTTCTGGCTTTGGCTTCTCATTGTCCATATCAATGAGAGTGACTGCTAACACAAGTATTGGTCCCACATCCTGCCTTTGACCTTCGGGACATCCCAGCACTGAGGTAAACATGACCAGAGCCCTATCTCTTAACTGCTCACTGTGGACTTTATTCTTCACAGGGGAGGGTAGGTACCAGCCATTGGGACCCAGGTTGTACTCAAAGCGACTTTTCTGAAGCATCCAGAGATAGAGCAAAGCTGGACAGATGTTCTCTTCATCTTCTCTGATTTTCTTGGGTACCGTTTGCTTGTCATGCCTGCACCTGTGGAGATTGCTGTTGTGGTCCGATCGTAAGTTCTGGGACGCACAGAGCTGTGTGAGTGCAGATTCCCTTCACACAGCGCTGAGGTGCTCAGTGGCCTGCAGCCGAGATGCCCAGAGCATGTCAGATTTCCCAAGCAGGACCTCTCTAGACATCTCTCAGCATGTGCTGCTGTCTCTTACGAGGTAGAAACCCTGAACACAAGCTTCCAGAATTAAATTGAACCCTTTAGTTCCTGGTCTCAAAGGATACCCACAGCTGTGATGGGAAGTTCTGCCACACTTTGTTTAAATGTATACTTTATATTATTCATTCATAAATAAAAATGGAATCCCGCTACATGCTGTTTTACAGTGCCGGGTTTCTGGCTGGTTGACTTTATCCGGTTAGAGCCAACTCCTCCCAGAGCTTATGTTCAGATGGCCCTGCCTGGCGAAGTGACACCCAGGAGAGGTTACAGCCCTTTGTAATGGCTCTTTCTCTTCCACTGATTTTACCCAATGGGGAAAGAAGTCTTTTTGTTTATCACAGATAGGTCCAGGCATTTTCCAAGGCCAGAGCTGAGCTGCATCCAGCATCTCTGGGGACTGGCTGCATTGTCCCCGAAATGCTGACAAATCAAGGGTAGTCCATATCTCTTGGGCATATTGTTGATTCTGCAAAAAATCAGGAGAGCAGATATAAAAGAAAAAAAGGCCCGCCCCCAGAGGCCTTGCAGGTGTGGCTGCTGTTTCACGTGTGCATCAGTAGGTGGCAGAGCGGAGGGCAGTTACGTCTACGCTCAAAGTTCTGACCTGTGAACATCTGAATGTTGTGTAGCCTAACATTCCCTAATAAAAGTGAGCCTGAGATGACCACCAGTCCAGCGCCACGGGAGACAGACACCCTCACATGGGGCCTCTTGGGCAGGTGAGGAGCAAGCCTGAGATAACCATCAGACTGGTGCCATGGGTCCCGGCCAGGAAACTAGCCTGAGATGACCATCAGTCTGGCCCCACTTGGACCTTGGGAGTGGAGGCAAGAAGGCCATATTTGAGATGCCTCCTGCCTGGGGCCATAATATGCGAATGAGAGATAGCATTCCTGAGACCACTCCTGAGATAACCCCAGACACCCCAGAGACCCTGGGCACCTCTAAGAGGAGCAAGTGGTGGAGAAATGAAAAAGAAAGAAGGGTGTGGGTACAATGAAGAGAGGCTGAACTGGAGATTTAAGGGTATCGGGGAACAGCCTGATGTGAGTAACCGGTGATGTACCATCTGGGACCATGGTGGGATCCTGGCCTGTGCTGCCACCAGGAACCATGTCTGGGTCCGTGGCCCTGTAGTAACGGGTCTGCTACCACCAATGGCCAGGCCGATGTCCCTGGTCTGACAGGTTGATGTCTGAGGGTTATGCAGAACCGGCCCCACCCCTCACCTGATCATCATGGCAGAGCTGGTCCTCTAAGGGGGATATGGGAGTAGCGGCCCTGACCCTGCCCTTAGCCAGCTGCAGTGCTTGGGAGAGCAGGCCCATACACTGCAGGAGTTGCGGGTGAGCTGGCCCAAGGATGTGAGCGTAGGAGAGCAGACCCTGACACTTATCTTCCTTGTGGTGACATGGATAAGGGACTGATCCCCTCCCCTCTTTGCTCTCAGCACCTGAGGAGAGCTGTCCCTGCCCCTCACCTGCTGCAGCTCTCAGGAGAGCAAGCCCCCACACCTCACCTGGACAGCACAGTGGAGCTGACCCTGATGGCCAGGGCTCAGGTGAACTGGCCCCAAGGGTGTGAGCACGGGGGAGCTGGCCCAGCAATTTTTTCCCCCTTTGGTTTTTCAAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCACTTTGTATGTAGACCAGGCTGGCCTCCAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGCTGAGATTAAAGGCGTGTGCCACCAGTGCCCGGCTCCAGCAACTTTTTTGTGGTGTGGCAACTTGGGTGAGGGAGAGATGCCCTCCTCCCTCACCTACCCTCACCCCTTGCCACCTATGGCCCCAGGGTCGTGAAAGTGGGAGAACTGGTCCTGTCCCTCACCAGCTGCAATGCTCAGGAAATTGGGCCCTGCATCTCCCCTGGGTAGCAAGGTAGAGCTGGCCAGGATTGCAGGGGTTGCTGGTGAGTCGGTTCTGAGCAGGAGAGCCAGTGGACTGACTGAGCAGCTCTGCTACTTCTAGGGCCCAGATCCAGGGCTTTGAGTTGGCTCACCCCAACATCTACCGTCATTGATGAACTGCCGGGGTGCACAAAGGGGCCAGTCCTACAGATCCAAACTTCAGGATCTCCATGACACAGGGCAACAGCAGGCTATCTGAGAGGCGTCCCAGTGAGGAATCAGCATTGATAGAGTAATGGAAGTCAGAGAGGCCTCTTATCAGACTGATGAATTCTTGCAATGAACATTTGCAAGCAAAGACGTGTGGACAAGAGGGTACACTGTGTGACACTGGGACACACTACAGCTTCTAAGAAGAGGTTTTTTTCTCTGCTGGGGGTGGGTCGAAAGGGTATAGGCTGGGTATGAGAGAGGAGGGGGACACGAGTGGGACTGGGGTGCATAATGTGAAATTTACAAAGAAACTAATAAAACTTTAAAAAAAAAAATGGTACTGGTGTGATGGCTCAGCAGTTAAGAGGACTTGCTGCTTCCAGAGGACCTGGGTTTGATTCTCAGCACCCACACGGTGACTCACAGCCATCTGTAAGTCGATCGGGTTCCAATACTCTCTTCTACCCTGTATGTACTAGGCATGAACATGGTAAACCCACATACATGTAGACAAAAGACCATACCGATAAAAATAAATCTTTGAAATTTCAAAATAAAAAAAGGTCCAAGGCAGGCTCCAGGGACCAACATCTTTCTGAAGAGGTAGCATGGGGAAAGGCAGATGTATACCTGGGGCAGTGCCTGTCGCCCGTCCGACCCCCATCAGCTAGGGCCTCAGTCACATGGTGGATTTTTAAAAGTTTCGGGAACTTCTGTGTAAGGGTGTCCCTAAAAGTTCCTTAGCAGCCTCTGTTTGGAGCTATGCTGGCTCCTATAGGCTTATGCAGCGCCCTTCCCCCGTGCTGAACACAAACCTTCCGGGTGGGTGTGAATCTGGACGGCCCTTCAGACTAAGCTCCTTGGGCTCCATCCTGGCATCCAAACCTGCCTAGGTTTTTTGCCTGAAGTCTGAGCTTAGCGCTAACCCAAGACAAATAGTGTCAAGGCAGCTCACCCGTCGGTTCGGCTGATTTATTTGACACAAATAAGCTCGCTGACCTGATTTAGCCTTGCGGGGGACCACAGACCAAGTCAGGCATGATCTTAAGGCTCTTGCTATTTGGCTGCAGCCAACGCGGCTCAGTGCTGGTGGCTGACGGCACGCACAGTGGTAGTTGCTGACTTTGCCACTTGGTGGCGCCAGTTGCATAGGTTTTTTTTCTATCCCTGGTGTGAGCCTGGTGTTGACTGCGGATCAGACTTCCCCAGGATGCCGCCAAAACAATGACTATGGGTAGAGAGCTATCTGGTGGCAGCAGCAAATCAGGACTCCTTCCTAGACCTGGCATTTTGGGGGGACCAGGGCCCTCAGAGGAGGAAATAAACCTGCTGGCGATGAGCTACTGTCTCTCGGCGCCCCCCTTCTCAGCCTCGGTCCCGGCCAGGACAAAGCATAGGTAGATCTTCTTAGCCATCCTTCAGTGACAGGGATTAGGAGCACGAAGGGACCATTCCCACTACGCCTTCTCTGAGGTGTTCATCGCTTTGATCTCCTAGGTGGAAGAAGATACCGACTCTCTGTGGTTCTTCTCCGTTCAAGGTCACACACAAACCATCTGCCTGGTGAGAAATCCAGACCAGGTGTCTCCAGGTATCTCCGGAGGGCACCTCGTGAGCATGCCCTGTGCGACATGGTGCTAAATAGGTCGCTTATGATTCAACTGCCACCCACCCTCCTGACCTAGGTAGGTCTTCTCATCTCTGTTTTATGGACACAAATTCAGGGGAGATTTGCACTGGCTTCCTGGCGTTAGCCAGGGGTACCCTCCACTGGGAAGACAGCTCCTGCTATATGGGAAGTGGGGTCCCTAGGAGTAGCAGGTTAGGTGTTTGCCCTGAAATTGGCCTGGGATTGGGACTATTGTTTAAGCAAAAGTTTACACTAAAAAGGTTTTTTTTTTTTTAGAAAAAAAAAAGACATTATTCATTATTTTTAGTTGTGTGTGTGTGTACAAGTGCAGGTTCCCACGGAGGTTAGAGGCCCTGGAGGCCCTGGAGCTAAGTCCCAGACAGTTTTTAGCCACCTGACATAGGCACTAGGAATAGAAACTGGGTCCTCTTCTACTCTTGACCACTGGACCTTCTCTCCAGCCTCCTTTTCTATCCCCTGGTCCTCGACTGTCCTAGAAGGGGAACTTAGCATTTTAGGCCAGCTCTGACAAGTGGCTGCATCTTACAGTCCTGTGGTCCTTGTCATAGGCTGAGTTGTGAGTCCCCAAAGTCCTAAGTTGCAACCTTAGCCCCATATTGTTTTTTAGGAGGGAGGGCCTTTAAGGAAATGATCGATGGGTGGGTGGGTCGATTCAATAAGTAAAGGCTCTTGCTGCCAAGCCTGACAACACGATTGTGACCCCTGGGACCCACATGGTGGAAGGAGAGAAACCACTCCCAATAAGTTATCTTCTGTGTACAGTGGCACACCTATACATAAACACATGCTAGGAAAATCATTGTGATTTTAAAATAGGAAGGAGAAGAAGGAGAAGAAGAAGAAGAAGAAAAAGAAGAAGAAGAAGAAGAAGAAGAAGCTGAATTCAGAGGAAGCACTATGATAAGCCTAGCTCAGTGTCCTTACAGGAAGAGAGAAAGAGAGAGGGAGGGGGAGAGGGAGGGGAAGGAGAGAACGCTGCAGACATCATCGGATCCCTGTAGCATCTTTGCTAGCCTCTGATTCTGCATGCTGCTTTTCCTCCATCCCAAATGTATCTAGCAGATACATTTCTGCAAAGCCACTAACAGAGGTATCTCCTACATCCCTATGTCCCAAAGTCCTACAGCCCCACCCCCTGCCCACTCCATAAAACCCGTACTGTGATCTGCTTTACACATGAGGGTAACGAGCCAGAAGCAGTGAGGTGAGACACAACCACTTCTCATTTCTCACTCTTTCCTCCCTCCCAGCTTCTTAGATCCCTGGACTGTTGCTTTTAGTGTGTGTAGGTGACAGGGGAAATGGGGGGGGGGGGCTTCAGATAAAGACAACCCTTGCTCTTCCTCAGGTTCTCTTTCCTACTTTCAATCTAATCATATC
GF3 -1 -1 1 GTGAGTGGCGCCGAGTGGCCTATG
GF4 -1 -1 1 GTGAGTGGCGCCGAGTGGCCTATGCACG
EX52 -1 -1 1 GACCGGAACCTCCTCCAGGTTCAAGACCGG
EX32 -1 -1 1 CTTGAACCTGGAGGAGGTTCCGGTCTCTC
GR3 -1 -1 1 CTCAATGAGTAGTTGGTGTCACGGATAGTC
GR4 -1 -1 1 GCTCAATGAGTAGTTGGTGTCACGGATAG
Comments:
Created
Target: 2803Ensembl ENSMUSG00000032840 -- 118705679 -- 118705835 ENSMUSE00000290475 -- ENSMUSE00000290475
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 7 oligo candidates from u/d regions
AOS Produced 13 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:5:1:152537259:1, start 118705678: end 118705835
Found 7 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
REJECT (1) gap pair G5:5:118702679:118703678_95 G3:5:118712335:118713334_737
ACCEPT (0) gap pair G5:5:118702679:118703678_95 G3:5:118712335:118713334_402
ACCEPT (0) gap pair G5:5:118702679:118703678_95 G3:5:118712335:118713334_180
REJECT (1) gap pair G5:5:118702679:118703678_89 G3:5:118712335:118713334_737
ACCEPT (0) gap pair G5:5:118702679:118703678_89 G3:5:118712335:118713334_402
ACCEPT (0) gap pair G5:5:118702679:118703678_89 G3:5:118712335:118713334_180
REJECT (1) gap pair G5:5:118702679:118703678_80 G3:5:118712335:118713334_737
ACCEPT (0) gap pair G5:5:118702679:118703678_80 G3:5:118712335:118713334_402
ACCEPT (0) gap pair G5:5:118702679:118703678_80 G3:5:118712335:118713334_180
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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