High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by dk3
Valid After Annotation
not done
Comment Type Comment Detail Edited User Edited Date
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 113504
Chr: 5
Strand: -1
Status: Ordered
BACs: RP24-71N1 : RP24-338G24 : RP24-152O16 : RP24-333N8 : RP24-79N11 : RP24-166O19 : RP24-373I1 : RP24-370H5 : RP24-70N10 :
Instance: Plate 120: Well C06: BACs RP24-71N1 RP24-152O16 RP24-333N8 RP24-338G24
Oligos on GRCm38:
Type Start End Length Seq
G5 120616404 120616453 50 ACTGCAGTAGTGATGCATTTTTAAAGGACAGCATGGAAGCGTGGAAGATA
U5 120610394 120610443 50 CAGTGCACATCCCTCCCATCCCAAAACAACCAAATATAAATGTACTTTTC
U3 120610302 120610351 50 AAGACTGGCGGCCTGCAGCCTGAGTTTGATCCTCCGCATGCACGTAGTAG
D5 120608880 120608929 50 ATGTGTGTAGAGTTGATGGACCGGGCGGGCAGACAGAGCCTTGAGGTCTT
D3 120608791 120608840 50 ACGAGCTATATCCCCGCTGCCCAACAAGACAATTGCTAGCCAAGGACTCT
G3 120604798 120604847 50 GTTTTTTGAATTAATTTGTAAGTACTGGGGATCGTGCTAGGCAAAGGTTT
Oligos:
Type Start End Length Seq
D5_D3 121058850 121058888 39 TACTATGTCCCGGGGTGGACAAGAACCACTCACAAACCC
G5 121066413 121066462 50 ACTGCAGTAGTGATGCATTTTTAAAGGACAGCATGGAAGCGTGGAAGATA
U5 121060403 121060452 50 CAGTGCACATCCCTCCCATCCCAAAACAACCAAATATAAATGTACTTTTC
U3 121060311 121060360 50 AAGACTGGCGGCCTGCAGCCTGAGTTTGATCCTCCGCATGCACGTAGTAG
D5 121058889 121058938 50 ATGTGTGTAGAGTTGATGGACCGGGCGGGCAGACAGAGCCTTGAGGTCTT
D3 121058800 121058849 50 ACGAGCTATATCCCCGCTGCCCAACAAGACAATTGCTAGCCAAGGACTCT
G3 121054807 121054856 50 GTTTTTTGAATTAATTTGTAAGTACTGGGGATCGTGCTAGGCAAAGGTTT
G5_U5 121060403 121066462 6060 CAGTGCACATCCCTCCCATCCCAAAACAACCAAATATAAATGTACTTTTCCAAAAAAGTCTATGTGAGCTCAGCAGAAATACATTATTTTTGCTTGAATAGTTCCCCTCCTCCCCAGAATCTCCTTTAATAAATTTTTATGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCAACTGCTCTTCCACAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCACCCGTAATGAGATCTGACACCCTCTTCTGGTGCTGTCTGAAGACAGTTACAGTGTACTTATGTACAATAATAAATAAATCTTTGGGCCGGAGCAACCAGGGCTGAGAGAGTGGGACGGACCAGAGAAAACAGGGTTGGTCCAAGTGAGCGGGGAAGACTGGAGCAAGCAGAGGTCCAGGTCCTAAATTCAATTCCCAACAACCACATGAAGGCTCACAACCATCTGTACAGCTACAGTGTACTCATATACATAAAATAAATCTAAAAAAAAAAAAAAAAAAAAAAAGAACCAAGGCGCTTGGCCATGGCCACACAACAGGTCCCTAACAAGCTGTTCCAGGAGACCTGACGGCTACTCTGGTGGTCAGTTTCAATTGCCAACTTGGCAAGATCTGGGATCATCAGGAAGGAGAGTGGCAGAGAGCAATCAGCTATGTCAGGCTGGCCCGCTGCCATACCTACGATAGACCTTCCCCTTAGTACTAGTGTTTTACCTGCACATGCCTGGGGTGTCAGAAGCGGGGGATGGGGGTCAGATACCCTGGGACTGAATAAAAGCAAGAGAGTGTGCTGAGCATGCAATGTGACCAACCATCTCCACCCCACACTGCTGTGACTTCTTATAACCAAGGAGAGCAACTCAAACCCTTTCTTGCCCAAGTTCCTTCTGCCAAGAGTATTTTATCACGGCAACCAGGAAAGGGACCAAGACAGCCACCCTTAAAACAGGAGTGCACCCCCCACCTTACCTGTATTTGTTTTTCTTCCTTGGTGTGAGGTTTGGGGTGCTGCCCCTGGAAGAGGGGGACTCACAGTCTCTCTTGGCCAGAGAGCCCTTCGGTGGTCCTGGGCCCACTCCTCTGGATCGGTTACAATTCTGCACCCAGGGTGGGTCAAAGTCTGGTTGGGCAGGTCTGGTTCTTGCTGGGCTGCCAAACAGGGTCTCATCCACATAGGATGCCCTGGCCTTGACCCTGTAGCTGGTGGGACTGCGGTGCTGAAGGTGGAGGGCTTTCATTCCGCTGCTGGGCCGGTGCCACCGGGGTCTTCACGTTGCCCAGTGGGTGTGGTGTGCCTGACAGACTCAGCCTCCAGGGCTCCCTGTAATAGGGGTCGGAATGAGCAATCAGCTTTTCCAAGTAAGAGCAGCAGCTTGGAATAACAAGCCCAAGGGCAAAAAGGGCTTTTTCTTGCTGAGGGAAAGCGACTGGTTTTTCTCAAAGGACTACTGTTGTTCAGTGGACACCTTGGAAGTGCTTCACTTTCTTGCCCGTCCCCGACACCCAATTTCAGTAGTCCAAGGTGGCCTGAAACTCACTATATTAAACTTTATTATATAGCCAAGAATGACCTTTAACTCCGGATCCTCCCGCTTCTACTTCCCACGGAGAACTGCACGATCGCCTGGCGTCTGAGGTGCATGGGTCAGGCTAAAGCAGGCACTGTCTTTTTAGTTGTGAAGACGGGAGAAAATGCCGTCAGCAAATATTTAATGAATAAGTAAATACACAATTACACCCTACAGTGTATGCTCAACAAATATAGTACGACTGCGGGCGTGCAGGGGAATGGCACCAACCAGACAAACTAATTAGGGTGGAAGAGGGAAAGGGGCTCCCAAGCTACTGTCGCCCCCCATTCCTACCTTGTGCAAAGCGCTTTGCAAACGTGGTGCGTTCCAGTCCTCGCCAACCAGTCTCATACGAAATGCATTTTCCCCGAGTCCAGCTCAGGCTCCCAACAGCAGAGTAGCAACGCACCGGCCAGCTTACAGAGCTGAAAGCGCAGGCGTCACGGGATCTCCATGCCTGGTGCTTTTTCTCCTGGAGCCTCCGGCCAGAAAGCTCTGTCTCCGCCACATTTAAAGCCTCATAGCACAGAATCAGCAAGGGCGCCACAGCGACACGCGCCCACCCGCATCCCTTGCTGGGCAACCGGTGCGACGCCCCACCCCTGCGCACTGGTACGTCCCTATACCCCGCCCTCGCGCTGATGGTACGTTCCACAGGCCACACCCCCAGCCTCATCTGTACATCCATTAGGCCCCGCCCCTTGGTAGGGTCAACCTTGCGTGGTTGGTACATTCCTCGAGTTTGTCCACACGTTCAGTTGTTATGTCCCTCAGGTCCTGCTCTCTTTACAGTTACCACATCTCTCACAGCTTGGCATTCGCGCAGTTAGTAGGTCCCTTAAGCATCTATCACGGTTCAGTTGACACATTCCGCCCCCACCAGTTGGTATGTCCCTCAGGCCTCGCCTCTGCCCGGCTGGTACACCACTTAGGCCCCGCCCCCGTTCTGTTGGTTCCTCCTTCTGGCTTCGCCCATTGATAAAGCCATGTTGACGCTCCGCCCCTGCGAAGTTGGTTGGTTTCCTCAGGCCCGGCCCCGCCCTACGGGTGAAACCTGGATTCTCGACGCCGCTCTTGCGTCTCACAGGCTCCGCCCCCGCGCAGTCGACGCGTCCCTTAGGCCCCGCCCTCTTCCGGGTCTAAGAGCCCGGCCCGCATGGCTGCTGGCAGACGTGTGGGACCTGGCCCGCCGTCGCGTCCCACCATGGCGCCGTGGAAGAAGAAGAGGCTGCGGAAACGCCGAACTGGGGCTTCCCAAGGCCGCGACAGCGACTCGGATGACGGCGAGTTCGAGATCCAGGCGGAGGATGACGCCCGGGCGAGGAAGGTAAGAGCGCGAGGGGGCTCCCTGTTCGGGCTCTCGGCCTGCGCCCTTCCGCTTGTCCGTCTCACTAATCGCGGTCCCTCATTGGGTCCCTTCGCGTGGGGGACGGGCTCGAGTTTACGCAGCCAATAGAAAGTGCAAGTGCGGAAGGTCCGCCTGAGTTTGGGGCGGTCTTAGATGAGAAAATTAAAGGGAAGGCCGGACCACTCTTCTGAGAAGCCGGTTTTAAACTAAACTAGTATTTACGGACTTATGTCGTGCTCTAGCTATGCCAGGCTCACCAGCAATGTCAGGGAGGACAGCAGGATTGGAACTGGGGATGGTCCGGTTCCAGTTGTATTCATCTTGGGGGATAGGTGTGATCACCAAGTGGGGCACAAAAGCCTATCGTTCATGACGCCCATCGTCTTGTGCATAGCGGGTATATCTGGTTCTCCATTTCTCCTTCCCAGCTCAGTCAATGAGCTGCACTCGTTAAGTGGTCAGTAAATGTGCAGAGACAGCTAGAGGCAATGAGAACGCTGGTGTTCATTGGCAAATACAAGAAGGCTGCTCCCGTGGTTCAGACCAAGGGTTTTGCTGAGGCCCAGTCCGGCACAGGCTGGCTGTAGTAAGACGTAATAAACTTTACAAGTCAAAACCCAGCCTGTGGGCTCTGAATGTAGCTTTGTTGATAGAGCGCTTGCCTGGCGTGTGCAGAGCCCTGGGTTTGAAGGCCATCACCAGGTAAAAGCAGGTATTGTGGCACACACCTGTAATCTTAGCTCCCAGGGTAGGAGTGGTGGCGGCAGGAGGATCAGGAGTCCAAGGCCACCCTGAGCTGGTAAGAGCGCCACCACCAATTCTTAATATTTTGCTGTACAAAAAAACCCAAAGGCTCCTCCAACCCGGGAAACACATGCTTATTAAAGGGCTTGTCGCACACCTGATGTAAATATGAACGGGAGAGCAGCCAAGTTCACACTGTACCATTACCCTCCACCAAAAATAAATAAATAAAGATGAAAAAAAGCAAGCCGGGCAGTGGTGGCACACGCTTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGTGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGATAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAAACAAACAAACAAATAAACAAACAAAAAACAAAAACTAAAAAAACAAAAAGAAAAAACCCAACATCTTTGTGCGGTGCAATGAAACCAGAATATTACATTGGGTGCAGAGTCTGTTGACTTAGGATCAGTGGGGAAAGCCTTTTTCTTTCTTTTCTTTTCTTTTCTTTCTTTTTTTTTTTTTTTGTTTTTTGTTTTTTGTTTTTTGTTTTTTCGAGACAGAGTTTCTCTGTATAGTTCTAGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCTTCGAACTCAGAAATCCGCCTGCCTCTGCCTTCCAAGTGCTGGGATTAAAGGCGTGCACCACCATGCCCAGCTGGGACAGCCTTTTTCATTCCCCACCCCCAACACACACACCTTTAGTTTGGGCAGGGTTAGAGACCAAACCTAGGGCCCTGTCTGTGCTAGGCATGTCTGTGTCTTTCAAGTAATCTTGGCCTAATTTATCTTCAAACAAAAATTCACCTGCCTTTACCTCCCAAGTGCTGGGATTAAAGGCGTGCGCCACCACACTGCCCTGCTTTTTTTGTATATCTTTATTTAGTCATTGTGTTTTGATGTGAGACAGGGTCTTGCTGTGTAGCCCTGGCAGGTCTGGAACTCACAGAGATCCTCCTGCCTTTGCCTCCGGAGTGCTGGTTACTGTCATATTGAGGTGACTGTCATAGCCCATCATGTTAAAGCACCAGGTAGGGTCTTGTGTAGCTCTAAGTAGCCTTGGACTCAGGAGGAGACAGGGATGGGTTTGATCTCTTGATCCTCCTACTTCTCTGGTGCTGGGATGACTGATGCGTGCCACCATGTTCCTTCCTAAGACCCACAAGAAGTTATACAATCACCTCTCTCATTAGAATCTAAAAGGTACCTGATGAGTGTCATCAAAAGGAGTCTAGTGAAGGAACTGGCTCTGGGGCCACCCACCTGCCATTTCAGCTCTCAGAAGGTAGACGCAAGAGGGTTGGGGGTTCAGAGGACCAGCCTGGGCACAGGAAACCCTGTCTGGAAAAGACCAAAAACGAAACAGAAAAGTTATACAAAGACACATAAAACATGTTTTGTTTTGTTTTTAAAGATTTATTTGTTTTATGGATGTGAGGACACTGTAGCTGTATAGATGGTTGTGAGCCACCATGTGGTTGTTGGGAATTGAATTTTAGGACCTCTGCTCACTCCAGTCAACCCTGCTCACTCTGGTTGGCCCCGCTTGCTCCAGTCAACTCCACTGGCTCAGTCCCTGTTCGCTCCGGCCCAAAGATTTATTTATTTATTATATATAAGTACACTGTAGCTGTCTTCAGACAGCGCCAGAAGAGGGCTCCAGATCTCATTACAGGTGGTTGTGAGCCACCAGGTGGTTGCTAGGATTTGAACTTGGGACCTTTGGAAGAGCAGTCAGTGTTACTTGCTGAGCCATCTCGCCTGCCCTGAAACATGTTATTTTTAAGCCAGAAAACAGATTGAGAAGTCAGAGAGTTGGCCCAGGAGGTAGAAACACCTGCCTGTTAAACCCGGTGACCTGATTTGATCCCTAGAACTTGCCTTACAAAGCTGGATATAAAAGCAGATCCGGACTCAGTTCACCCAGTTCCAAGGGATCCACTGCCCTCTTGTGGTCTCCTTGGGTACTACACACACATGCTGCCTATAATATCTGCAGGCAAGACATCTGTACATTTAAAAAGATGGATATCACCGTATCTGCAACCCCAACTTCCCTAGGGTGGAGATGGAAAGTAGAGACAGGAGAATTTCTGAGAAGCTCACAGGCCAACCATCCTGGAGTGCACCAATGGCAGAAATGATGGACCCCACTTAACCAGGGTGGAGAGATAGAGCTGAGTCTTAAAAGTTGGCTATGGCCACAGGACACTCGGGCCTCCACCCACACTGTGGCTTCCACGCACGTGTGCATGCACACTGCAGTAGTGATGCATTTTTAAAGGACAGCATGGAAGCGTGGAAGATA
U3_D5 121058889 121060360 1472 ATGTGTGTAGAGTTGATGGACCGGGCGGGCAGACAGAGCCTTGAGGTCTTCAGACCTTAGCAGTCACTGACATTTCTGTCACATCTAATCACATCCTCTGATCACCTGCTGTCTAGTTTACCCCTATTGAGGTCGACTGAGACCTTTACTTAACGGGGAAAAAGTAACACACTACCGATTTTAGTGATCCAAATTCATTTAATACATGGAATAAATACATGAATACTAAAATAATGTCTTCCCATGTCCCACCCACTTGGGTCCACGCACAGCCCAGGACACTTCCTCAGAGCCTCCAGATCACTGTAAAAAACATACTCCTTTGCAGGGAGCTGGGTGGGGCTTGGCACCGATGCAGAAGGCGGCTGAGACACCGCTAGAGAGACAACATCTCAAGGACCCCAAACACCCTTCAGAGAAGGCTGGGGACATCCTGAGCTTGTGGGGACTAGGACAATATGGCCCATCCTCACCGTGGCACCCCGTGGAGGCGTTTCCAGCATCTGGAGGCCCTGGGGACCGTTGTCTCTATAGGACCCCACCCGAGCTTGAGTCCTGGCTTTGGGCCAAAAGCATCCCTGACGAGAATCTCACTTCCAAGGAGGCTTCGGCTTGGGAGGACAGGCCCCTCGCCGAGGGGGAGCTGGCCCCGAAACACGACCTGAGCATGCCTTGGAAGTGACCAGGGGACTCTGGACAGTCGCCCCTGATGTGTTAGGCCGGGGACTCCAAGGCCCATTGGTCTGGGGAGCACTGGAAGCTGGATGACCCGTGCTGGGGACAGTTAGGTGGGTGAGTGAATGGGACCGCCTCTGTCCCAGAGAGCAGGGAACACACAACCCATCCTGGGATGACATCTGGGAGCCTGCAGTCACTCTGGGCTCTGTCCTGGAGGGTCCAGCTGGGTGAATGGCTCGCAGAGGGGTCTCCCTGGGGCAAGGTGTGTGGCTGCCCCTAGGGGTGGCTGGTGGGGTCCAGAAAAGGGTCCGGAGCTTTGCAGCATCCCCCACGGCCATCCGAGACCCCTCCTTGCTAGTGCCAAACAGTGACTCATCGCAGTAGGATGGGGCATGGCCAATCAGCCTGAAAAAGGGAAGGGCACTGTGACCGGTGGTGGCGATGATCCTAAAGCCCTCTGCAACCTCACTTCCCACGATAAAGTAAGGCAGCAGAGCCTGCCTGGTACCCACAAATAAGCCTGACATTCTTTAATACAATGACAGGTATAGGTCCAAAGCAGCTCAGGGCTTCCCGCTCTCTTCTCCTGGAGACAAAAGGACAGATGACAGACCCCTTCCTGAATCTGCTCAGCTCCAGATTAATGGGAGTGATTAGGCCCATTAAGTGCCACATAAACAGAAGTAGCAGACACTGTCGCTTAGGGACAGCAAAAAGGCTCAGTGGGTAAGGGTGCTTGTGGCCAAGACTGGCGGCCTGCAGCCTGAGTTTGATCCTCCGCATGCACGTAGTAG
D3_G3 121054807 121058849 4043 GTTTTTTGAATTAATTTGTAAGTACTGGGGATCGTGCTAGGCAAAGGTTTTGAGCCTCACCTCCAGCTGGGAGCTCACCCTTTAAAAAAGATTATTATTGTGTGTGTGTGCATGTTGTGTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTACCTATGGACATACAGGTACCACAATGCCTGTATGGAGATTGGGAGAACCCTGCTATCAGCTCCCTGCTGCCCCCTTGTGGCGCCAGAGAATTAGTTTAAGTCCTAAGTGCCTCTACCACCTACTGAGCCAACTTAAAGGGCCCTGCCTGGACGTTTTTGTTTTAAGAAAAAATACAAAGTGGAGACAGAAATCGAGAACTGGATCCAGAAATATGACATGGAGATGGGCGAGAAGCAGGTACGTGCCGGGGGTTCCTCCTGAGGGATTGGGGGAGGGAGGGTCGGGTAGGAGCTGCTCAAGGTGGCCTAGGTATGAGACTCCTCCCCTGGAGACCCTTTGAAAGAGAGAAGCTGAAACTGCTGAAATCCAGATTCATCCTGAAGCACGTGGCTCCACCTAATAGCCACCTCCCATGGTCCTCTCACAGCCCGGCTCAGCTCCTGCCTGCTCCCGGTTCACAGGCGAGCCCTCCTCCCCCACCCCCACATCACTCTGCACCCAAAAGGTCCAGGTGCCAGGCTCCCGAATAAAGCAGGGAATCGAGACGGTTCTCACTGTCGTCTGAAGGTTTCAAACTGGCATCGCTCATGAGCTGTGCCCCCTGATAGATCTGTAGATGCTGGTGGGAGGGAGGGATAGAGAGGAGGAGGAAGGAGGGCACAGAGTAGCTGGGAATAAGAGGAAAGGGAGAGGGAGAGGGAGAGAGCGGGGGGGGGGAGGGGGGGAGAGGAGGAGGAGGAGAAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGACGACTCGGACCAAGAAGAAGATATTTTTTAAGGGCCCACCCCACTACCCTTCCACGAACTACTTCCTCCCGGAAGGGCTCTCCTCCTGTCTGTTTAGCGATCAATGCATCCACTGGGTAACCCAGAGATGAGCTTGGAGCTTTCCCGATTCAGTCACTACTCAACAGCGCCACCTAGTGGGTACCAGGTCTCCAAACCAAGTGTCTGTGAAGGACCAGTTCATACTGAAACCCGAAGGTCTCTTGATCCCAGAAACTGGCCCTGGTATTTGGAATTGTCCCAGTGTTTCAAGTGAGGAAAACCAGAGCTCTCCTAAAGGTGGAGAGAAGCTAACAGATGGGATGCTAGCTCAGTAGGATGCTTGCCATGCAAACCTGAGGACCCAAGTTCGCATCCCCTGCATCCCATGTGAGAAGTGAAGGCGGCAATGTGCACCTGTGGTCACAGTGCTGGGGAGGGCACAGGCAGGCTGATCCTGGGAGTTTGCTGTCCAGCTAGCTCAGTCACATCAGCAAGCTCCAGGTTCAGTGAGAGAGCCTGGCCTCAGGAAGTAGGGTGGAGAGCCCGAGGAGGACACGTGACCTTGACCACCAGCCTCCACCTATAGTCACACACACACACACACACACACACACACACACACACACACACACACACACTGCATTTATTCACATGTGCATTTACACAAGAACAAAAGCACATAGGTTTGCACAACTCACCACCCTTGGTCCTCCCTCCTTTCTGAGATCTGCTCAGCCGGGTCTTTCCTGGAGCGAACCCAGGTATAAATATACAGACAGGCTCTGTATCCTACCTGGCTGGGCTGGAAGCCGGGCATGGGGAACGGGGAGGCCATGCCGGGTGCTGCGCACCTCCAGCCATGCCCCTCGCATAGGACGAATACGAGGACCTGGAGAGCATCCACAAGGAGGAGAAGCTGCAGCTGGAGGAGCTGAGGGAAAGGCACGCGGTGCTGGTGGAGGAGTTCTCTCAGATCCGGGCCGAGAGCGAGATCAATTCCAAGAAGCGGGTGGAGGCAGAGCGCGAGATGGTGCGCATGGTGAGAGCCGCCACGCTCATCCAGGCGGTGTGGAAGGGCTACCTGGTGCGCTCCATACTCAGGTCCAAGAAGAAGAAGCGGGGCAAGGGGAAGGGCAAAGACAAGGGGAAGGGCAAGGAGAAGCCCAAGGAAGAAAAGGCCAAGGAGAAGAAACCCAAGGCCAAGGGCAAAGGCAAGAAGAAGTGACCACATCCTGGTCTGGCCCCGCCCCTGTTCAGCTTCCACCACGCCCTACCGGGTCCCAACCAAGCCCCTGTTCTGTCCCAGCCCTTCCATCCCTGGCTTTAATGATGGCTAAATTAAAAAAGACAAACAACAAAACCCGTTTCAAACCACACTCAGTGGCTCACCCTTCAACAGACCTGTCTGTCCCAGGCTGAGGCTGAGGCCGTGGGCGCAACATCCTGAGGAGGGCGCAAACTTCGGATCCCCATCCTACCACACCTTCATTGAAAAATGAGGCCCTAGTCTTAGCTGAACTGTCAGGAGGGCAGCAGGGGGGTGGGGGGTGGGGGAGGGGTTGTGTGCTCAGACTGTTCCATGAGTTTTAAAAAGTACCAGTTGCTAGAATTTCACGAATTGAAAATTCTCCCATAGAAACTTAACAGCCCTTTCTGCAAGGATGTCAACACCCCTGACATCCCAGTAGTTGGGCCCTGTGTCTCTCCATGCAGCAGCAACCACTCTGCCAGCTTTATTTCCATGTAGCCCAGAGTGGCCTTGAACTTAAGGTGATCCTCCTGCTTCTGATTCCTAAATGCTGAAATCACAAGCACGCGCAGCTTTAACTAGCTGACTCCTTTCCACTCTGAAAAACAAAGTATGTGGGAGGGGGCAAGGGAGGGGGACCCTAGTGGCCCAGCCATGGCTTTAATCCCTTCAGTCAGGCAGAGGCAGGCAGATTTCTATGAGTTTAAGGCTAGCCTGGTCTACCAAGCTAGTTCTAGGACGGCCAGAGTTACATGGGGGCAAACCCTGTCTTGCAAAAAAAAAAAAAAAACAAAAACAAAAAACAGGAAAAAGGTTTATTTATGTGGATGTGCCTACAGAGGCCACAGCTGGGTGTGTAGTTCACTGGAGCTGGAGCTAAAAGCCTTGTGAACCACTTAAAGAGGGCGCCGAGATCTGAACTCAGGCCCTCTGACAGAGCAGTAACCCACGAGCCATCCCTTCCATTCCTACTTTGTTTTAATCTTAAAATGTGTGTATGTGTGCACATACCCATGTATGCCCATATATATATATATATAAAATCATGTATCATGTGTGCCTGGTGCCTGCAGAAGTCAGACGGTGTTGGCTCCCCTGGAATTGGAGTTACAGGCCTTCTGACGTGGGTGCTGGGAACCAAACCCAGGTCTTCTGGAAGAGCTGTAAGCACTCTGAGCTGCTGGTCCTCTCTCCAGTCTCCCCCCTTCCTCCCTCCACTTTTTGTGGTTCCTGTCCACAGCCAGACTCCAGGCCTGGTGTCAGCTAAGCTGGTGGCCTCACCCTGAGCTTCAGCTCTCCTTCAGTGTAGACGGAAGAATGTTTCTAGCCTGTGTCTTCTCAACATGGAAGGATGGGACAGACAAACAGGAGGGAAACCAACCAATTCTTCATGAAAGTTCTAGAAGCTTCAATACCCTACATGGCTAAAGCACAAAGCATGTCTGTGTCCAGGCATGGCTGGGGCACCTCTGCAGGTCATTATATATGTCCCTGGGCCTGGCTACCCCTAGACAGGGGTGAGATACCCACTGGGCTCTCTGGAATGTTCAGGGAGGGAGGGGGCAAGGAGAGGCTCAAGGTCCTAGGCCCATCATCCAAGGAAGGATGCAGGACCTTGGGTGAAGCTTGAGGCCCAGGGGCCCCTGCAGCCTGTGGGCAGTGCTGAGCACCCAGGGGTCTCTCTACTCGGCTCATCACTGAGCCCTGTTGTTTTGTTGGGCTGGGGGTTGCTGGTGACTGAATCCAGGTGCCATTCACATATGCTAGGCAAACACTCTACCACGAGCTATATCCCCGCTGCCCAACAAGACAATTGCTAGCCAAGGACTCT
U5_15 121060403 121075453 15051 CAGTGCACATCCCTCCCATCCCAAAACAACCAAATATAAATGTACTTTTCCAAAAAAGTCTATGTGAGCTCAGCAGAAATACATTATTTTTGCTTGAATAGTTCCCCTCCTCCCCAGAATCTCCTTTAATAAATTTTTATGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCAACTGCTCTTCCACAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCACCCGTAATGAGATCTGACACCCTCTTCTGGTGCTGTCTGAAGACAGTTACAGTGTACTTATGTACAATAATAAATAAATCTTTGGGCCGGAGCAACCAGGGCTGAGAGAGTGGGACGGACCAGAGAAAACAGGGTTGGTCCAAGTGAGCGGGGAAGACTGGAGCAAGCAGAGGTCCAGGTCCTAAATTCAATTCCCAACAACCACATGAAGGCTCACAACCATCTGTACAGCTACAGTGTACTCATATACATAAAATAAATCTAAAAAAAAAAAAAAAAAAAAAAAGAACCAAGGCGCTTGGCCATGGCCACACAACAGGTCCCTAACAAGCTGTTCCAGGAGACCTGACGGCTACTCTGGTGGTCAGTTTCAATTGCCAACTTGGCAAGATCTGGGATCATCAGGAAGGAGAGTGGCAGAGAGCAATCAGCTATGTCAGGCTGGCCCGCTGCCATACCTACGATAGACCTTCCCCTTAGTACTAGTGTTTTACCTGCACATGCCTGGGGTGTCAGAAGCGGGGGATGGGGGTCAGATACCCTGGGACTGAATAAAAGCAAGAGAGTGTGCTGAGCATGCAATGTGACCAACCATCTCCACCCCACACTGCTGTGACTTCTTATAACCAAGGAGAGCAACTCAAACCCTTTCTTGCCCAAGTTCCTTCTGCCAAGAGTATTTTATCACGGCAACCAGGAAAGGGACCAAGACAGCCACCCTTAAAACAGGAGTGCACCCCCCACCTTACCTGTATTTGTTTTTCTTCCTTGGTGTGAGGTTTGGGGTGCTGCCCCTGGAAGAGGGGGACTCACAGTCTCTCTTGGCCAGAGAGCCCTTCGGTGGTCCTGGGCCCACTCCTCTGGATCGGTTACAATTCTGCACCCAGGGTGGGTCAAAGTCTGGTTGGGCAGGTCTGGTTCTTGCTGGGCTGCCAAACAGGGTCTCATCCACATAGGATGCCCTGGCCTTGACCCTGTAGCTGGTGGGACTGCGGTGCTGAAGGTGGAGGGCTTTCATTCCGCTGCTGGGCCGGTGCCACCGGGGTCTTCACGTTGCCCAGTGGGTGTGGTGTGCCTGACAGACTCAGCCTCCAGGGCTCCCTGTAATAGGGGTCGGAATGAGCAATCAGCTTTTCCAAGTAAGAGCAGCAGCTTGGAATAACAAGCCCAAGGGCAAAAAGGGCTTTTTCTTGCTGAGGGAAAGCGACTGGTTTTTCTCAAAGGACTACTGTTGTTCAGTGGACACCTTGGAAGTGCTTCACTTTCTTGCCCGTCCCCGACACCCAATTTCAGTAGTCCAAGGTGGCCTGAAACTCACTATATTAAACTTTATTATATAGCCAAGAATGACCTTTAACTCCGGATCCTCCCGCTTCTACTTCCCACGGAGAACTGCACGATCGCCTGGCGTCTGAGGTGCATGGGTCAGGCTAAAGCAGGCACTGTCTTTTTAGTTGTGAAGACGGGAGAAAATGCCGTCAGCAAATATTTAATGAATAAGTAAATACACAATTACACCCTACAGTGTATGCTCAACAAATATAGTACGACTGCGGGCGTGCAGGGGAATGGCACCAACCAGACAAACTAATTAGGGTGGAAGAGGGAAAGGGGCTCCCAAGCTACTGTCGCCCCCCATTCCTACCTTGTGCAAAGCGCTTTGCAAACGTGGTGCGTTCCAGTCCTCGCCAACCAGTCTCATACGAAATGCATTTTCCCCGAGTCCAGCTCAGGCTCCCAACAGCAGAGTAGCAACGCACCGGCCAGCTTACAGAGCTGAAAGCGCAGGCGTCACGGGATCTCCATGCCTGGTGCTTTTTCTCCTGGAGCCTCCGGCCAGAAAGCTCTGTCTCCGCCACATTTAAAGCCTCATAGCACAGAATCAGCAAGGGCGCCACAGCGACACGCGCCCACCCGCATCCCTTGCTGGGCAACCGGTGCGACGCCCCACCCCTGCGCACTGGTACGTCCCTATACCCCGCCCTCGCGCTGATGGTACGTTCCACAGGCCACACCCCCAGCCTCATCTGTACATCCATTAGGCCCCGCCCCTTGGTAGGGTCAACCTTGCGTGGTTGGTACATTCCTCGAGTTTGTCCACACGTTCAGTTGTTATGTCCCTCAGGTCCTGCTCTCTTTACAGTTACCACATCTCTCACAGCTTGGCATTCGCGCAGTTAGTAGGTCCCTTAAGCATCTATCACGGTTCAGTTGACACATTCCGCCCCCACCAGTTGGTATGTCCCTCAGGCCTCGCCTCTGCCCGGCTGGTACACCACTTAGGCCCCGCCCCCGTTCTGTTGGTTCCTCCTTCTGGCTTCGCCCATTGATAAAGCCATGTTGACGCTCCGCCCCTGCGAAGTTGGTTGGTTTCCTCAGGCCCGGCCCCGCCCTACGGGTGAAACCTGGATTCTCGACGCCGCTCTTGCGTCTCACAGGCTCCGCCCCCGCGCAGTCGACGCGTCCCTTAGGCCCCGCCCTCTTCCGGGTCTAAGAGCCCGGCCCGCATGGCTGCTGGCAGACGTGTGGGACCTGGCCCGCCGTCGCGTCCCACCATGGCGCCGTGGAAGAAGAAGAGGCTGCGGAAACGCCGAACTGGGGCTTCCCAAGGCCGCGACAGCGACTCGGATGACGGCGAGTTCGAGATCCAGGCGGAGGATGACGCCCGGGCGAGGAAGGTAAGAGCGCGAGGGGGCTCCCTGTTCGGGCTCTCGGCCTGCGCCCTTCCGCTTGTCCGTCTCACTAATCGCGGTCCCTCATTGGGTCCCTTCGCGTGGGGGACGGGCTCGAGTTTACGCAGCCAATAGAAAGTGCAAGTGCGGAAGGTCCGCCTGAGTTTGGGGCGGTCTTAGATGAGAAAATTAAAGGGAAGGCCGGACCACTCTTCTGAGAAGCCGGTTTTAAACTAAACTAGTATTTACGGACTTATGTCGTGCTCTAGCTATGCCAGGCTCACCAGCAATGTCAGGGAGGACAGCAGGATTGGAACTGGGGATGGTCCGGTTCCAGTTGTATTCATCTTGGGGGATAGGTGTGATCACCAAGTGGGGCACAAAAGCCTATCGTTCATGACGCCCATCGTCTTGTGCATAGCGGGTATATCTGGTTCTCCATTTCTCCTTCCCAGCTCAGTCAATGAGCTGCACTCGTTAAGTGGTCAGTAAATGTGCAGAGACAGCTAGAGGCAATGAGAACGCTGGTGTTCATTGGCAAATACAAGAAGGCTGCTCCCGTGGTTCAGACCAAGGGTTTTGCTGAGGCCCAGTCCGGCACAGGCTGGCTGTAGTAAGACGTAATAAACTTTACAAGTCAAAACCCAGCCTGTGGGCTCTGAATGTAGCTTTGTTGATAGAGCGCTTGCCTGGCGTGTGCAGAGCCCTGGGTTTGAAGGCCATCACCAGGTAAAAGCAGGTATTGTGGCACACACCTGTAATCTTAGCTCCCAGGGTAGGAGTGGTGGCGGCAGGAGGATCAGGAGTCCAAGGCCACCCTGAGCTGGTAAGAGCGCCACCACCAATTCTTAATATTTTGCTGTACAAAAAAACCCAAAGGCTCCTCCAACCCGGGAAACACATGCTTATTAAAGGGCTTGTCGCACACCTGATGTAAATATGAACGGGAGAGCAGCCAAGTTCACACTGTACCATTACCCTCCACCAAAAATAAATAAATAAAGATGAAAAAAAGCAAGCCGGGCAGTGGTGGCACACGCTTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGTGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGATAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAAACAAACAAACAAATAAACAAACAAAAAACAAAAACTAAAAAAACAAAAAGAAAAAACCCAACATCTTTGTGCGGTGCAATGAAACCAGAATATTACATTGGGTGCAGAGTCTGTTGACTTAGGATCAGTGGGGAAAGCCTTTTTCTTTCTTTTCTTTTCTTTTCTTTCTTTTTTTTTTTTTTTGTTTTTTGTTTTTTGTTTTTTGTTTTTTCGAGACAGAGTTTCTCTGTATAGTTCTAGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCTTCGAACTCAGAAATCCGCCTGCCTCTGCCTTCCAAGTGCTGGGATTAAAGGCGTGCACCACCATGCCCAGCTGGGACAGCCTTTTTCATTCCCCACCCCCAACACACACACCTTTAGTTTGGGCAGGGTTAGAGACCAAACCTAGGGCCCTGTCTGTGCTAGGCATGTCTGTGTCTTTCAAGTAATCTTGGCCTAATTTATCTTCAAACAAAAATTCACCTGCCTTTACCTCCCAAGTGCTGGGATTAAAGGCGTGCGCCACCACACTGCCCTGCTTTTTTTGTATATCTTTATTTAGTCATTGTGTTTTGATGTGAGACAGGGTCTTGCTGTGTAGCCCTGGCAGGTCTGGAACTCACAGAGATCCTCCTGCCTTTGCCTCCGGAGTGCTGGTTACTGTCATATTGAGGTGACTGTCATAGCCCATCATGTTAAAGCACCAGGTAGGGTCTTGTGTAGCTCTAAGTAGCCTTGGACTCAGGAGGAGACAGGGATGGGTTTGATCTCTTGATCCTCCTACTTCTCTGGTGCTGGGATGACTGATGCGTGCCACCATGTTCCTTCCTAAGACCCACAAGAAGTTATACAATCACCTCTCTCATTAGAATCTAAAAGGTACCTGATGAGTGTCATCAAAAGGAGTCTAGTGAAGGAACTGGCTCTGGGGCCACCCACCTGCCATTTCAGCTCTCAGAAGGTAGACGCAAGAGGGTTGGGGGTTCAGAGGACCAGCCTGGGCACAGGAAACCCTGTCTGGAAAAGACCAAAAACGAAACAGAAAAGTTATACAAAGACACATAAAACATGTTTTGTTTTGTTTTTAAAGATTTATTTGTTTTATGGATGTGAGGACACTGTAGCTGTATAGATGGTTGTGAGCCACCATGTGGTTGTTGGGAATTGAATTTTAGGACCTCTGCTCACTCCAGTCAACCCTGCTCACTCTGGTTGGCCCCGCTTGCTCCAGTCAACTCCACTGGCTCAGTCCCTGTTCGCTCCGGCCCAAAGATTTATTTATTTATTATATATAAGTACACTGTAGCTGTCTTCAGACAGCGCCAGAAGAGGGCTCCAGATCTCATTACAGGTGGTTGTGAGCCACCAGGTGGTTGCTAGGATTTGAACTTGGGACCTTTGGAAGAGCAGTCAGTGTTACTTGCTGAGCCATCTCGCCTGCCCTGAAACATGTTATTTTTAAGCCAGAAAACAGATTGAGAAGTCAGAGAGTTGGCCCAGGAGGTAGAAACACCTGCCTGTTAAACCCGGTGACCTGATTTGATCCCTAGAACTTGCCTTACAAAGCTGGATATAAAAGCAGATCCGGACTCAGTTCACCCAGTTCCAAGGGATCCACTGCCCTCTTGTGGTCTCCTTGGGTACTACACACACATGCTGCCTATAATATCTGCAGGCAAGACATCTGTACATTTAAAAAGATGGATATCACCGTATCTGCAACCCCAACTTCCCTAGGGTGGAGATGGAAAGTAGAGACAGGAGAATTTCTGAGAAGCTCACAGGCCAACCATCCTGGAGTGCACCAATGGCAGAAATGATGGACCCCACTTAACCAGGGTGGAGAGATAGAGCTGAGTCTTAAAAGTTGGCTATGGCCACAGGACACTCGGGCCTCCACCCACACTGTGGCTTCCACGCACGTGTGCATGCACACTGCAGTAGTGATGCATTTTTAAAGGACAGCATGGAAGCGTGGAAGATAGCAGCACTGTGTTTGGTGAGCACTCTATTGAAGGAAATGGCCTGTGTCTGTGTTGGTCAGCATAGTAGCCACCAAACACGGCATCTTAAATATCTGTGTTCTGTTTACACCACCAGATCCCTGGTATTTAGCTACAGAACCTGGGTTCATCTTATATTTATAGCTACATGGCGGTAGGCTTAGCATCTCTTCTCAGATGGATGAAATAATGAACTAATTTCAAAATGAAAATGAGAAAGGAATAGAAATACGCCAGATACAGGTCTGTGATTCCAGCTGCATAAGAGACTGAGGCGGAGGATTACAGGTTCAGGGCCAACCTGGGCTACGTGTGGACTTCAGGAAGCCCAGCTTCAAGCCCCAGAGCCTCTCTGTAGAACTAACCTGCGCCTCTGCCCCAGCCTGGTCCAAGGGGAGCTCATCAGAGTTGCCGAACTTCCAGGCCTCCCACCCTTGTGGTCCACCAGAGCTGGAGTGAGACCATCTCTCTGTTCCTTCCCGACAGCTGGGCCCTGGCAGAGCCTTGCCCTCATTTCCTACCTCAGAGTGCGTATCAGATGTGGAGCCCGACACTCGGGAGATGGTGCGAGCCCAGAACAAGAAAAAGAAGAAGTCTGGAGGCTTCCAGTCCATGGGTGGGTAAACAGGCTGGGTAGCCAGGGCCACGGGGGTGGAGGGGGTCTCATTGTGCTGTCTCCGCTTAGCCACTGATGTCTACCTTAGGCCTGGCAGTGTGGTAGGTGCCAAGTCGCCTGGGGCAGTACAGACAGTCTTCTAAGGCAGGCCTGGAAAGGGGTCAGCCCTGCAGGCACAGGAGGTGGCAGAGGGCAGCTGTGGATGATGCAGTGGTGTCAGGACGCTTGTGTGGGAAGTGCAGCTCAAGTGTTCTGCTCTTGACTGACCAGTAGCTGCCATGGCAAGAACGGAGACACCCACTGAATGCCTTTTAATAGCAGTACAAAGGAGCTAGATATGGTGGTGTGTGCCAAGGCTACATCATAAAACCCTGTCTCACAACAAAACAAAAAATACCTGCAGCCCATGGGACAGACAGAAGCTTATCTACAATATATAAAGAGTTCTTACAAATCAATAGGAAAAGGACAAAAGCCCTGGGAGAATGGAGTAAAGAAACCGAGTAGACTCACAGAAGTGATGTAGATGGCTGTTTAGATCAAGAGCACAGTGCTAGCTCTTGATAATGAGGGGATCAAGCAGAAAAGGACATGGGGCCATGGCAGAGATGAAAGATTGACCAGGCAACAGGTAAAACTGTTTATTGGTCTTTGGGCCTTGTGTTGGCTCCCAGCTCCTCGGTCCAGGTCTGTTTGATCCCCAAGGGCCACTCCTACCCACACTGGCCCCCATTTCCCAACACCCATCCCTATCACACCTGTTAGAGTGGCCCAAGCCTGTGATCCCAGCACTCCCCTGGGCAGAGGCAGAGACCATAAGTTCAAGGCCAGTGTTGAACACATAAGCAAGGCCCTTTTCTTAGACTGAAAAATTGACATGAGCAAATTCTAGCAGTGGTCAGGCAGGGCATCCCTGCCTCAGTTGAGTGTCTTCTACTCTTTTAGTTTTCTGCTTTCTGTTTCTCTCTGTGTGTTGGCGGACAGCGTAGTCCTTTGTCCTGTAATGCCTGGTGGCTGCTGTGGGCCCTGACTACTCCCTTGTCTTTTCCAGGCCTGAGTTACCCTGTGTTCAAGGGGATCATGAAAAAGGGCTACAAGGTGCCGACGCCCATCCAGAGGAAGGTAAGGAGCTAGATACCAGCCATGAACAAGTGGCTGCATGACAGCTCTTTCTGGACCCAGCTGTCACCTTGCTTGTCACCTTGTGTGACTGAAGCTCCACATGGCTTAGCCATTTCATGGGGGGGTGGGGGGAGCAGAGCTGGGGTTTGACTGTGGCTCTCTGCCTCTTGCCATGCGGAGCCTGCCCAGGTAGCACACAAGCACCCCATGATCACCCAGCACGCTGGCTGCATGGGAGACAAGGTGGAGACCCAACTTGGGAGGCAGAGCCTCCTAGCACAAGGAGTCAAGGATGCTTACTGGAGGTGTGCTGACAGGCCTGTGGCTTACCTGTGCCTCAGCCATGCAGGGGCCAGAGCTGACAGTGCCTCCATCGTTATGTCTGACAGACCATCCCCGTGATCTTGGATGGCAAGGATGTGGTGGCCATGGCCCGGACAGGCAGTGGCAAGACGGCCTGCTTCCTCCTCCCGATGTTTGAGCGGCTGAAGGCACGCAGTGCACAGACGGGGGCTCGAGCCCTCATCCTCTCACCCACCCGGGAGCTGGCCCTGCAGACCATGAAGTTCACTAAAGAGGTATGCACGCGGAACTGGGCCTGGTGGGAAGGCTGAGGCGGCTGGCCCTCACCTGCCTTTGATCCCTCTGTCTCCCTCTCTCCACAGCTAGGCAAGTTCACCGGCCTCAAGACTGCCTTGATCCTGGGTGGAGACAAGTAAGGATTCCTTACCCGGAATCCCAGCTTCCCTCAGCAGCCACGCCCCTGTGCCTGAGGGTTCCCTTTGTGGGCCACAGCTGCTAGTCAGTAACAGCAGCTTCTCTGTGTGTTCAGACAAGCTGGCCAGGGGTTCGCGTGGTGGAAGCTGGGACAGGGCAGAGGGACCCGTCGGCTTGCAGTGTTGGGTCAGGGAGTTTGATGAGGCTTTCGTTGTATAGGATATGCCTTTCTCTGGGTGCTGAGGCTGCGGTTAGTCTGCCCTGCTTCTCTGTTCTGGCTTCTACTAGGGGCTGTAGTATGTGATTGTCAGACAGCTGAGCCTTCCCAAGCTATGTGGAGCCAGGATCCCCACTTCTACAGCTGCACCAGGGACTCATGAGAACAGACACTTAGCCCTAGGGGAATTAGGGTTCTAGGTGAAGCTGGTAATTCCAAGCTGAGTCACTAGGTGGCGCTGTGCGTTGGCGGCACTTTGCTGGTGAGGAGTCTAAGGTTCTCCCTTGTTTCTCAGCGGGGGCAGGGCCTTGACCAGGTGTCTCACAGTAAGCATTTCTCACTTCCATACCTATAGTGTGTGCTCCATGGCGAACTGTGGGGTTGCTTGGCTGTGGGGGAGGGCAGGGGAGGGACAGATTCAAAAGCACTGTTTCTCAGGCAGCCCTTGCTTTTCCCCTAGAATGGAAGACCAGTTTGCAGCCCTGCACGAGAACCCTGACATGTGAGTTTGTTGATGGGGGTGATGCTCTGGGGCCCCTTCCCAGAGAGTGGCGTGGAAGGTGTCGTGGGCTGTGGTGGTCCTGGGCCGGGAGTGACCTGGCCTGTGTATTGCTTCCAGAATCATTGCCACCCCTGGGCGTCTGGTGCATGTGGCTGTGGAGATGAACTTGAAGCTGCAGAGTGTGGAGTATGTGGTGTTCGATGAAGCAGACAGGTGAGGCCACGGGTATACCCCACCCCCAACCCCGAGCAACAGTCAAGGTTGTCCCCAGCGCTCAGGAAGCAAAGAAGAGGGAGGTCACAGGAGACTAGGTCTGCCCAGGAGATGTGAATGTATGTTTGAGTAGTCCCATCCCTGGGCATCTGCTCATCATCATTGTAAGTTTATGACTTAGTGCTGAAAAGAACGTTACATCCCATGCTGACTGTGGGCAGTAGAGAGGGCTCACTGAGGATGCGTGAGGCTGCGTTCATTCCCCTACAGCGGAGAAACAGACACAGGAAGATTCACAGTGCCACGTGGCCGGGGTTCTGAAGTGAGGGGAGCCAGGGTTCCAAGCTCTTTAGGGTAGGAGCCTACCATTGAGCCTCCCTGCCCGCCATTCCCTGCAGGCTCTTTGAAATGGGCTTTGCTGAGCAGCTACAGGAGATCATAGGCCGCCTTCCTGGGGGCCACCAGACGGTGCTGTTCTCAGCTACACTGCCCAAGCTGCTGGTGGAATTTGCACGGGCAGGTGAGCCAGTGAGTGGGGCGTGGTGCTGGACTCGGGGTGGGGGTGGGGTCTGCCCCTTCATGACCCCTGCAATCCCCCCCGCACCCCAACAGGCCTCACAGAGCCCGTGCTCATCCGCCTGGACGTAGACTCCAAGCTCAATGAGCAGCTCAAGGTGAGGTGGTGTTGGGCTCCTTCCTCAGGCTAGTGCCGGGGCAGAGTCGGCCAGTCCATGACCAGGAGTCCACATCCCTCCCTCTGCCCGCAGACCTCCTTCCTCCTTGTGCGCGAAGACACCAAGGCTGCTGTGCTCCTCTACCTGCTGCAGAATGTCGTTCGGCCCCAGGACCAGACTGTGGTGTTTGTAGCCACAAAGCACCATGCGGAGTACCTCACAGAGGTGAGCTGGGGCTCTGGGGGATGGAGAAACCATGGGCTCTCAAACCAGCTTCATCCAGCACCCAGAGTTGCACTTCCAAGGGAGGCCACCAGAGGGCAGCAATAGCGCGCCCATGAGCCTGGGTGGGGCTTTTCCCACACGCTGACATCAGAAGACACTGGTGAACTGTCAATGCCGTTGTGAGGGTGCAGACCCTGTCAGCACACCCAAGTTGGTTTTGGCCTGTCTTGCTTTCTGTTCCAGTCATCTGCAGTAACTCCTAGTGGACCTGGATTTTTGGGGGGGTGAATTTCCAGTTTGTGCCATGCCTGACACCTAAGTATAAAGCTAGCGTGTTAGCAGGGTGGCGTGCCCTTGGCCTTCTCTGTACATTCACACCTGTGTGACCCGCAGTTAGATGCTTGCATCTGGAGAGTGCCAGCAGCCTGGAAGGCTGGGGGAGGGGCTGCCTAGCCACTGTCTCGCCCCAGTAGGCACACCTGGGCACTGTTGAGGGCACGGGTGCCTGAGTCTCATTTACCCGGCATGTTAACGGCGCCTTCCTCCCCCGAGCGTCGGAGACTAAAATAGTAACTTGGAGAGAGCGGTTGGTGTTCACAGGCTTCCTGTGTCTCAGGCTCGTTCCTCAATGGTACATTAACCACGTAGTCCTGTGAGGCACAGAGAGACTGAGTAATTGGCCCATGGTCACACAGGATCCAGCAGCCTTGCAGAGCTAGGAAGTTAGGTAGGAGCTGCTTTGATGCTGACGCAGGGAGCAGTGTTCAGACCCAGAGTGCCCGAGCCTGGGCCCAGCCTCCAGCCCTTGCTCCTTCCTGCAAGCTTCCCCCACCCTCACCGCCTTTCCTCTGCACAGTTGCTGATGGGCCAGGGTGTGAGTTGCGCCCACATCTATAGTGCCTTGGACCAGACGGCCCGCAAGATCAACTTGGCCAAGTTCACACACAACAAATGTTCCACCCTCATCGTGACTGACCTGGCCGCCCGGGGCCTGGACATCCCACTGCTGGACAACGTCATCAACTACAGCTTCCCTGCCAAGGGCAAGCTCTTCCTGCACCGAGTGGGTGAGGCGCCTAGCTGCACTGGGGGCCTTGGGGGCGGGCGGGGGGAGGGGTGCTGTACCGTGCTGGCAGCTGGGAATTTGGGGAAACTGAGGCCTTAAGTAATGTCCCTTGGGTGGGTTCTGACAGGCAGCCTCAGCTCCCAGAGCTTCCTTTCTTTCGGTGTTGGGAACAGAGCCCATGGTGTCGCTCAGGCTGGGAAAACTGCCACAGACCAAGTCGTGTGCTGTGTTCATGAATGCAGGCAGATTAAAGTGTGCATGGGAGAGAGGCCGGGCATGGGGCTTCTTAGAGTTGTTCTCTGTGTGACTGAGTGGTTGAATTCATACGCACATCTGCCGGGCAGAGGAGGGGCAGCCACTGGGCGTTGGTGTCCTGATACTTCACTGTAAGGCCCGCAGGAGCCCCTGACCATGAGACCCAGTTCCTGACCTGGCTGGTGGCTGCCTGCTGTCTCCAAGTCTCTATTGCTCTCTGAACAGCTGGCTCTCCCATCTGGGGAGTTTCCAGAGCTACTCGGGGGAATTCCTCAGGTGCCACATGACAGGCCACTCCCTCTGAAACCTGCAAATGAGGGGAGGCATGGGAAAAAGCTGGGCGTGGCAGGGCTTGTTTGTGGTCAGAGCACTTAGGATGCTGAAGCAGGAGGATCAGGAGTTTGAGGCCAGCCAAGGCTATATAGAGTAAGCCTAGCTGAAGTGTGATACCTGCCATTCACTCCAGACCCTGCTGGAGGCCTGTTGGGTTGTTCCCTACCAGCTGCTGCTGTTCAAGGCTCTAGCATGTACATGTGTTTTCATTTACTGTGACGTCCTTCTTCATGGGTGGCACATCTTCAGTAAAGGGCCCCAGTGGTGACTATTTTTACATGATGCACTATACCATCTTTGTTGTTGCTAATTCAGTCTACAGTGTTAAGTGGAAAGTTCCACTGGGAATATGTAAATGAGCAGGCCTGGCTGTGTGCCAGTAAAACTTTATTGTTCAAGCAAGTGGTGGGCCAGGTGGAGCTTCCCCCGTCCTGGCTCTGTGACTCCCTGGTGGTCATCGGCTTGTTGACCCGTCAGCCCTCTGACCCAACACCTTTCAGGAGTTCTGGCCCAAACCTTTTTTGCTGAGAATTTGCAGTCTTAATTAACCCTTGGGGGGAAAAATATTCCCAAATTGGCAGTGCCCAGGACTTTTGAGTCCGGGTCACAAGAGGTACTTGTCCTGCTTTTGATCACTCTACCAACCAGTACCCACCCCTGACCACCTACCCTGTAGGTGGGGGCAGCAGTTCAGGAAAGGCCCTGGCCTAGCTCTAGCCCTCAGGGAGTTGGGGTCCCTTGGCTGAAACTCAACTTTCCCCGCTACAGGCCGTGTGGCCCGAGCAGGCCGAAGTGGCACAGCCTATTCTTTGGTGGCCCCAGACGAGGTCCCCTACCTGCTTGACCTACACCTGTTCCTGGGCCGCTCTGTCACCCTGGCCCGTCCTTGTGAGGAGCCTTCAGGTGAGGCCAGGCTGGACTGGGCTTGGGTCCCCATGGACCATGGAGATCATGAGCAGGCTGGGGTGCAGTGGTCTGACCACAGGAGATGTCTGCTGGGTCTGACCGTACGGCCTGGGGTGCTGGGGCTACCCTTGGGCTATTGTTGCCAGAGTGGGGGGTCTGGTTGCATATAATACTCTAGCCTGTATCTGTTGCCCTGGAGTCTGTAGGCCATGAGGGATAGACTGTAGGCTGACTCCTCAGTGTGCCCTGTCAGTGGCAGATGCGGTTGGCAGGGACGGAGTGCTGGGTCGCGTGCCCCAGAGTGTAGTGGATGATGAGGACAGCAGCCTGCAGACTGCCATGGGGGCATCCCTGGATCTTCAGGGCCTGCACCGCGTGGCCAACAACGCTCAGCAGCAGTATGTGCGCTCACGGCCAGCGCCCTCGCCTGAGTCCATCAAGAGAGCCAAGGAGCTGGACCTGGCAGAGCTGGGCTTGCACCCACTCTTCAGTAAGCTGGGCAGGGTGGAGGAGGTGAGGGTGGGCTGGCCCTTCCAAACCCATCCTGGCCTTACACACTTCCTGCCCCTGCAGGCTCATGCTTTGAGGAGGGAGAGCTCCAACGCCTGAGGCTGGTGGACAGCATCAAGAACTATCGCACGCGCACAGTGAGTGGACTCAGAGCCACTCGGGAAGGGGGTGGAAGGGGATGGACGTTCTTGTGAGCACGAAGCTTCCTCACATATGATGGTCCTCTTGTCCACTCATAGGCCCCTTACCCCTTGATCTGAAGCACTCCTGTGACCCACGGCAGGCCACCACTCAAAGGTGGATTGAATCCCCTGTGGGATGTGGTGTGTTCTCTGCCATCCATCCCCACCCAGCCTTGAGTCTCCCTTACCTGTTCATTCCTCCCGAGAAGCCATGGGCAGGCATGGAGAGCCCAAACATGTGGCCAGTGGCAAAGAGAGTGTGCCACGGGTAAACTAGTAACCACGGATAAACTAGTATCCAAGCCCAGTGCCCTCAGTATGAGGTCTGGGTTCTGGCTTTTCCTGGCTGTGTGGCCTTGGCAATATACCCACTTTCTCTGGGACTAGCTCTAATTGTGTGTAGCTGATATCTAGGGTCCCACCTCCCACAGTGAAGACTTGAGGAGCACGCATACAGGTCGCAGCTGGCTTAGGGTAGGGCTGGGGATTGGTGGGTATTAGTTGATAAACGTGGCATCCTTTTACAGACCATCTTTGAGATCAATGCCTCCAGCAAGGACCCAAGCAGCCAAATGATGCGTGCCAAGCGGCAGAGGGACCGGAAAGCTGTTGCCAGTTTCCAGCAGCGGCGCCAGGAAAGGCAGGAAGGCCCAGCTGACCCAGCCCCCCAGAGGGAGCTGCCTCAGGAGGAGGAGGAGGAGATGGTTGAGACTGTAGAGGTGCGGGCTGCAGGGGCAGCTGGTGCTGGGGACACACAGCTGGGTCCCAGAAGCCCACGGGACAGGCAGTGGTGCAGCCCCATCTCCCCATGCAGTCTGTGAAAGTGGGGTACCTTCTCAGTCTGCATGAGGGGGAGGGGTATGGAGACGAAGGCAGGAGGACAGCCACAAGTTCAAAGCCAGCTGAAGCTACAGAGTGGTTGTCTGGGATAACCAAAGCGCTGTCTCAGCAGTTAGGAGTCCATGCCCCACCAGCTGTCCCGGCTTACGCCTTTGATACCAGCACTTGGGCAGCACAGGCTTTTGCATCTCTGAAAATTCAGGGCCAGCCTGTTGACAGCAAGTCTCCATACTGAGACGCTCTGCTGTGACAGGGGATTGGCGTTCAGTTCCCAGCATCACATTAGGTGATCACAACTGCCTGTAACTCCAGTTCAGGGATCCAGCACCCCTGTGTAGGTACATACACACACACACACACACACACACACACACACACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATATTAAAATCTTAAGAAAAAAGACAGAACCCCTTTCCCAGATGTCGTGGCCTGGGCTGTGGCAAAGCGGCTCAGCCTGAGGTCCTGTGGGAGACGCGCCCCTTGGGGCTGGAGGGTGGTATTCCACCAGCATGGCAGTGGCTTCTCCTATCAGTGAGGCGGGGAGAGTCATAGTCTGAAGGC
D3_15 121043801 121058849 15049 ACTGACCAGAGTGTGCAGTGGAACTTGATGGAGCAGAGCAGAGACTCTATGGTTTAAGAGCTTTATTATAGAAATGCAGGGAAAAGAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAGGAAGGAGAAGAGAGAGGGAGGAGAAGACAGAGAGAAAGGGAAGAGGAGAGAGAAGTGAGAGAAGGACAAAGGAGTGAGAAGGGGGGCTGAGCACCCCTTTTTATGGTCTTCACTGTTGCTAGGTAACTGGGGAGGAGTTTAGCCTGAAGGTTCAGAAGCTTGGGCCATTGCTTATGTGACTACTGACCATGCTTCTCTTGTGAGGGCTGTGGGAGGTGGTACCTTAGGCAGGGCTGGAGTTCCAGGAGCATGAGGGAATGCCTGTTGTGTCATGTAGATGAATTATGACCATTGGGGTTCAGACCTCAGCTCGACTGGAGACCAGCCTGCAATTCCCCACAGTCCTGCAGGAAATGACAAGGAGGCAGGGCCACAGTCTCCTCATCTACTGACAGCTGTTTACTGAGCATCTACTATGTGATAGCGGCTTAAGATAAAGTCAGTAGGAGTAGGAGTAGCATTGTGTGTGCCTGTGTGCCTGTGTGTACGTGTGTGTGTGTGCTGGCATGCCATGGCACAAGTATGGAGGTCAAGAAGGACTCTCAGGAGTTGGTTTCCTCCTTCCGCTGACTCAAACTCAGGTCCTCCGTGTTGGTAAGCAGCATCTTTAACCACTGAGCATGAATGGATGCGTTCAGCATGATTAACTGGACTCCTGTACCTCTCTCTCAGCTCCCTCTTCTTGTCTGTGAATCAGAGAAAATAACCATGACCTTGCAGGGTTATAAGGACCGAAAGCACTCATCCCTAGGAAGTACTCCTTAGCTCAGCAGTAGCGATGCCCACCTTTGAGCCCAGCTCTCAGAAGGCAGACACAGACTGATCTCTCAAGGCCAGTCTGGTTTACAGGGTGAGTTTCAGGACAGCCAGGGCTACACGGAGAAACTCTGCCTGGAAAACAAACAAAAAACAAAACAGAGAGGACTTCTTCTTCTTTTCTTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCAAACTCAGAGATCCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGAGTGCGCCACCATGCCCAGCTCAGAGAGTACTTCTTGATTTTTATTTTGTTTGAGACAGAGTTTCCCTATATAGCCTGGGATGGCCTACAGTTCATTGTGTAGCCCAGGATAGCCTTCAACTCACTGTGTAGCCAAGGGTGGTCTTGAACTTTTCTCATCCTGCGCCCCCCCAGCCCCCTTCAAGAGCTGGGATCACTTGTGTATTTAGAACAGTTGGGCACATAGGTAAGTTCTAACACTGGCCTGTATTACAAAAAAAATTTCTTAAACTAAGACCTTCCCTCTCTACCTGACCTTCCCATAGTCACAGGTGATGGGGTTTCTTCTTCCAGCTTCTCTCCAAGTGTTTGAGGGGCTCTGTTAGTTGAAAACATTTTTTTTTTTCAAAATAAGAGTGCAGTAATAAATATCCAAGAGGCCTATGCCTATCTGAATGAAGTGGAGGCATATTTGCATGATAAAGATGAATCAATAGAATTCATGACAATTAAAGTCACAGTCCAGCAGCAGCCCTGCTTCACTCTCTGGGCAGGAATCTTACAAAGAGAGGCTGGGTTGGCAATGATTTCAATATGAAGGTTGGTGGCATCTTAAAGGACAGGGAGCCATCATATTAGAATTCAAGGAGCCGACTCTTGGGTCATTTTTGCTGGCATCATCATCTACCCCCTCGCTCCACATCGGCCACTTCTCCCATTCCCCCAGCTTTTTCACCCAAATAAGCTGTCAGGAGCTGATGGGCGTCTTGGTAATCAAAGAAAACAAGCTTGCCCTTTAAAGCAAACGAGGCAACTTGGCTGAAAGCAAATTGGAAGCATTCCCCCGTGGCTCTGAGCTCCCATCTCCTGGGGAAATCTGGGAGTTTTGATTGATGCCCAGGAGAACTCAGAGAACAACGTGGAAAAGTGAGCCCTTCTGTCTGTCTATCCAACACTTCCTGTCTTCATCCTGTGGCCTCGGGGCAGGGCAAGGGACCCTTCATCTGAGGGGCTGAGGAGAGGCACCAAAGCTGCCCATGACACTTTACAGTCAGGAAAAGGCCCCTAGGGAGTTCACTGTGGGATGAGAGAGCAAGGAAGAGGGAATGAGAGTCTCCCAGCGAAGCAAGATGGTGCCACCTCGGGCTGGGTCTTCAGCCTCTAAGGTCTGGCTCTGGGTGAGTTTTGATTGGCCAAGGAGCCTCCCATCCTTCACAGCAGACCTGGCACCCCTCTCCTCTGTCCATAGATGCGTTACTTAGTTACTGCTTTGCCGTCTGTGGCCTAGGCTCTTTGATTGCAAAGGTGATCAGCTGAGCCTCTCTCATGACAAACAACAGAAACAATGAAAGCAATGGGTGCAGCAATGAAAGCAACGCTGACCAACCAGGCTTCAGAGCTCAGACAGCAGCCAAGGAGTGCGCACACACAATTTTGGGGTCTGGATTAAAAGAGTCTTCAAGAAGGAGCACGGAGCTTGGCATTCCCCAACCGTGCAGTTTCTCCTCAGCCAGTGTCCCTACTCCCCGGATAAAGTGGGCAGGACTATTTGACAGACAGGTCCACCAAGACTGCAGTGTCCTTCAGCTTTCTCACCATGACAAAGCCCCCGAGGTGAAGGACTCTTAAGGGAGAAAGGCTGGTCTGGTATGTGGTTAGAGGCTCCAATGTTTAGGAGACTGTGGTGGGACTGTGGGTCACAGAGGTGTGTGTGTGTAAAACAAAGATACAGAGGCACAGTGTGACTCCTAATGACCCAAGAACCTTGAACTAGTTAAGGGTGATATAGTTACCAATGCCTTTCAGTCCCAACACTCAGGAGCCAGAGGTATAGGCAGGTGGTTCTCTTTGAGTTCAAAGCCAGCCTGATCTACACAGTGAGTTTCAGGTCCACCAAGGCTAAAACAGTAAGACTCAGTCTCAACAGACACCCTCCCTGCCGCCCCCGCCGCAAAAGGAAATCCCCACCAGACCCCACCACTTAAAGAAATGTCCCACCACCCCTGCATAGCTCTATGGGCTGGGAACCCACGCTTTAACACATCCATGGGGTTTTACACAAACCATGAGCATCTATGACAGGGAGAGGCTGCTGCCTGAGGGAAACTTGGCTGCCTGAGGACACAGACTTCCACAGACAGAGACATCCGTGCCATTGTCCCCTCCGGTGCTGGCAGCTTAAGACCTCAAGGAGTCCGAGATCCAAGTCTGAGGTTGAGGAGCCTAAGGTCTGAGAGCGAGTCCTCTGCCCTCCCCCAATCTCCTTCAAATACTCTCTCCAGGCTTCCCCAAGTCACAGAAGTGTGTAACACACCCTGACGAGCCCCTGCTGGTGTTGGCAGACCCTGTTTCTTTGGAGAAGAGAAAGATAGTGACCAGTTAATGAGGTCTGTGTGGGGATGCCAGAGCCAGTTAGCAAGGCAAGGGGCATGCAGAGGGGGCAGGCTGAGCACAGAGGAAGGTACTCGGCTGTGGATGGCATTCGTACAGGACACCATGACGTCCCAGGCTCACACTTCAGCTTCCCTGGACCTATAATGTAATATTAGCTGTCTGTTGAAGACATATCATGTCTGAGCCTTGTGCTCGAACCTAAGGAGTCTTGCCTTACAGTAAAGAGAACCCAGGCCCCCCTAACCAGACTTAGGTCTCCCAGCAAGGACTGCAATCCAGCCACTGTGCAACCAAAGTTTTAAGTCTTTTCTTTAAAGTTAGGGTTTCTTTGTGTAGCCTTGGCTGTCCTGGAACTCACTCTGCAGACCAAGGTGGCCTCGAACTCAGAGATCCACCTGCCTCTGCCTCTTGAGTGCCAGGATTAAAGGTGTGTGACCCTATGCCCAGCTTGCCAAAGAATTTGGCAAAAATTTCTGATGTCTCCATTGAGTTCAAGACAAACCTTTTACTTCGTAACAATTGTGGATAATATATATCCTCCGAATTCTTCTGAAAGCTCCCCCTGATGTTACTTTATAGGTCAGAACTAAGAAAGTAACATTGCCAGCCCGGGGTAACCAATGACCACACATGCCTGCAATCCTCAGGCTTTGGGGATGGAGGCAGGAGTTGCTACACAACAAGTCCTGGGCCAGCCTGGGCTACATGAGACTCTGTCTCAAACAGAAACAAATATGTAAGTCACTATCCATACAGTTCTGTTAACCAAACCTTGGATTTAGTGACATCCCAGATGTTACCCTGATGCCCTTTCTGTTTCAGAACAGAGCCCTGACCCTTTAACACAGTTTCTCATGTGCTACTGATCAGATCACCAGCCATAAAATTATTTCACTGCTACCTCATAACTGCAATGTAACTATCTTGATATGCAAGATATCTGATATGTGACCCATTCGTGGGGGGTGGGTCTTGACCCACGTGCTGAGAACCTCTAGTTCAGGACTTGATTTGATTTTCAGGAAAATGGACTAGCCTCCTCCCTCCCTCCCTTTTCTTTCTTGTCTTTTCTTCCATGTGTTGGCATTACCGGCCAGCCCCACAGCACCTGGTGAGTAATTATTTTGTTTGTTTTGAGACAGGGTTTCTCGATGTAGCGCTGGCTGTCCTAGAACTCACTCTGTAGAGCAGGCTGGTCTGGAATTCAAGAGATCCACCTGTCTGTGCCTCCCAAGTGTTGTGATCAAAGGCATGCACCACCACTGCCTGGCTTAACTTTATTCATTTACTTACTTACTTACTTACTTACTTACTTACTTACTTTTGGAGTTTTGAAAATATACTTAGGCAGGCCTTGAACTCTCAGCCATCACCCTGCCTGCGCCTCGTGGGTGCTGGTCTTAGCAGATACCACATCTCCTGAAACTTTCTGTACTCTGTATTTTGAAGTTATTGTTACAACTCTGCCCCAGTTTTCAAATCAATACCCCCAAAAAACTCACCCGGCCAGGATCAGGAGTGGTGGCTGTCTTGGGAAAGTACCTGCCACACAGGCATGGTGAGCTGGGTTCCATCCCCAGAGCTCACACGAAAAGCCAAGGACGTAGGCAAGAGTTTGTAATCTCAGCCTTGGGGAGGTGAACCCACTGGCTTGCTGGCGGCCAGCCTGCCCAAGCTGGTGAGGTTCCAGTTTAGCCAGAGACCCTGTCTCAAAAAGTAAGGAGACAGATGAAGAAAGACACCTGATAGTGATATCTGGCCTGCATGGGGACATATAATATGTAGAAGTGCACACATGCACATGTACACACATGCACACACTATGCATACCTGATCACAGATGCTCCCGGTGTGTGCTGGTACACAGTCATCCACCCTGCTTGGCCTTGCCCCTGTTCAGCTCTGTCCATGTCCTGTCCTATCCTGTCCTACTCTGTGTGACCTGTCCTCCTGTGTCAGTGTCTGCTCCTATTCTGTGGCCCATCCTGCTCTGTCCCTGTCCCGTCCTAATGTTCGGCATGTCCTGCTCCTTGCCCCAGACTCCTCCGCATCCTGGCCTGATGATGTGGTACACCAGTGGCCCAGGCTCTTGAGTTGGTTTCCTGCTGGTTTAGCATCAACGTGGGGGGGGGGAATGCAAGTTGGGGCTAGGACATTTCCACTCTCTGATCAGACAGTGCTTGCAGTCACAGCTGCAACTCCTCTGGGGTCTACTGCCCCTGGTGAGCCCAACACATGTGGCTTCCTGCTGCTGGAGCTGCTGGGTGACCTCAGTTGCCTGAAGCTGTCCTCTCTCTGTTTCTGCCTCTTGGGTAACCCTTGGAGGTTGGTCCCCAGAACAGAGGCTGCTTTTTGCCTGATAGATGTGCCTGCAGCTGCCCTCCACCCTCAGGCCTCCTACCCCCACCCCCGCTGGCTCAAGCACCATCACGAAGCCAAAAGATAAGCTTGCCAGGGTGGTACACGCCTATAACCGTAGCCCTCAGATCGGGAGGAAGGGACAGCCTAGGCTATAATGGACCATATCTCCAAACCAAACCTTGAAGACAAAAAAGCTAAAAGTAGCTTTGAGGTTCTGTACAGTGGTCATCATGGCCAATGGCGATGTACCTCACAGTCACCCCTTCCTCTGCAGGGTTACTGGAGGCTGTGAAATCCAGGAGAGCCTTGAGTTGGCGGCTGCCTCAGTACCAGATTATACCGAGGCAAAGGCAGCCTCTCCAGAAATGGCCTTGGACCAGGTCAGCATACCCCCTTCCTACCACGGACTGGCCATCCAAAGAATCCCACTGAGAACAGGTCTAGTCCCAGCAGAGCCGATGAAGACCTTAGTCCCATCCAAATCCAAGCTCAACACCATCGAGGCCAAGAGGATCATGTCAGTCCTGGATGAGGCCATACACAAGATAGAGCTGATAACCCTGATGTCCTACATGGAGTCCCACCCAGAGGCTCTGGAGGACACGCTGCCCGAGGATTTCGTGAGAGCCCTAAGAGAGCACTTGGACATTGGCCAGACCCTGGTGGAGAGGGCCAGCATCCTGCAGAGGAGACACAAGAAGCTGGAGGAAGAGGAAGAAGCCGAGGAAACTAGGAACCAGGAACGCCTACTGTCCCTAGAGCTGCACAAGGTGAACCTGCTGACGCTGGCGCACCAGTTCCGAGACTCCACCAAGACTGTCCTGAGGCTCGTGCTGGGCGAGCCTCAGTTCACCAGGCTGTTACAGGTACAGGCGCCAGGCAGAAGCCCGGGGGCCCAGTGCCTCCTCGACGGTCTGGTGGAGCTTCGAGGTTTCCTCTTTGAGAAGCTCCTCACCAGCCCCATGGAAGTCAGGGAGAAGAACCAATTCATACAGGACATCAGCCGGAGGAGCGAGAGGAACCAGGAGGTCATCGATGACCTCCAGGCTGAGCTGGCCAACGTGCTGAAGAACAAGGAGTCAGAGGTACACCTCCCTAAGGCATCCCCCAAATTAGGGGAGAAACCTCACCCAAACAGGCCAACCCTCTGGGATTTGCCTCTTCTCTCATGGGTTTCAATAGCTCTCTCCCTTCATTTAATCCTCAGAAATTGTTCTGAGCATTTTCCTAGGAGGTCTGTCTGTAGGAAAAGCCCAACCTCCGGAGCTCAAGGAAGGACAAGGGCAGGGCGCTATCAAGGGATGATGTAAGAGAACTAAATCCTGGCCAGAAGGACTAGGGACAGCGCCACTAAGACAGGGAGGACACAGAAAGCGGGATTGAGCCAGACAAGCAAGGCAGACCTTCCCAGCCAGGGGTTGGCAAAGTGTCTGTGTCACTGGCTAAGTGGGTCCTTTGTCTCGGGTAAGATAGACACCAGACATGTGGGGGAGGAGTTGGAGAGATGGCTCAGAGGTTAAAAGTGCAGGCTTTTGTCTGTTGGAGTCAGGGTCTCACTCTACAGCCTTGACTGGCCTGGAACGCCATAGAATCCCACCTGCCTGTGCCTCCCAAGTGTTGGGATTAAAGGCATGCGCCACCACTCCCAGTACACATTGCTCTTACAGAGCAACAGAGGTCAGTTCCCAGGCCCTCTGGCCAGCTGCTTGCAAACACTGTTTACTCCAGCTCTCCCGGGGAATCTCACGCCCTCTGCTGGACACCACAGGCACCTGCATTCACTTGCATGGGGCCAGGCATGGCTCCGTGAAGACAAAGCCTTCTGTGCGAGCCAGGGGATTTGAGTGTGGGCTCCAGAGCCACTCTGTTCCCTGAGACTGGGCAGGCAGGGACAGGAGTCAGTGGCCCACCAGCCTAGCATAGCCAACAAGCTCCAGGCCAAAGAGAGAGGTGTATGACAGTTGAGGCTGTACACACAAAGGCTGTACACACAAGCACACACATGCACACATGCAGGCCACTCTATAGCTGTGAACACAGAGCTGTGACATCCAAAGCTTCCGCAGGCAGCTTAAAGTGCACCTGTACCTGCATTCCAACCAGCCCCACCCCCACCCAGATTTTCCTAGAGAGCGTATTAGAATGAGTCTCAGATCTGTTGCATCTTGGCAGCTGTGGGTCGTCCATGGCTACTGTCTTCCTGCACACACTTGCCAGCTTTCTTTCTGGCTCCATATCCCATCTCCAAAGGGTCACACATCCCACACACTGGGCTCAGTGAGCTAACGGTCTCAACATGTCTCTGTTGTTAGTGTGGAAGCAGCCATGGAGAGTATCAGGACTGAAGGGCTGTGTCCCAATAATACTTTATTGACAGCTATGGCTGGCTGGATTTGCCCTGTAGGCCTTGGCCAACCCCTTTCCTAGGGGAGGGGAGCATGTGGTCCCAGGCCGGAGAGACGCATAAGCCCCTAGGGGAGGGGCACAACCAAAGGCCAGCTGTCCTGTGACTGGGAGTGGGAGGACTGCGTGGCTATTAAATAGGGCCCTGTCCTCATTCGTCCTGGCCTAGGTGGAGAAGGAGAACTTTGTGATCCAGGAGCTGAAGAACCATCTGCACCAGGTGTTCAAGTTTTCGGAGAACAGCCTCCTCCGCACGAAGCAGGAGGCCGAGAAGCAGCAGAAGGTGGATTTCCGGGCCTCCCAGGTCAGGCTGGCCAAGACGCAGCAAGATATCCTGGCGCTTCGGGCACAGTACCACAACCTGGTCATGGAGAACCGGGAGGCAGAGCAGGCACTAAGGAAGGTGCCCTGAGGTCTCAGGGGTACAGTGGGGGTGGGGGTGGGGGTGGGGGGCAGCAGGGCTGGCCCAGGGGTGCGAAAGCCCAAGATAGGTGGGATCTCCTGGGAGGTGGGAAGGGGAAGCAGCCAAACCAAGAGTTGGGTCAAGGAGGTCAAATCCCCATCCCAGAATTAGGGCACTAAAGCTCAGTGACCTCCCCAAAGTCCCTGGGCTCCCCTGAGCCCGAAAGGATGATGGCCCCTTCTTGAAGTAGCTCGTGACCCTCAGGCAAACTGAACAGGACCCTTCCGTGTCTGGTTCTCATGAGCTCTGAGGAAGCATAGCTGGAAGTGTGGGCACAGCGCGTGCTGTGAGCATGTGCCAAGCATGGCTGCTTTGTTTGTTCCCTGTCTCAACCGTGCTCCAACCACCTCTCAAGTCTCTTTCCGAGGACACCTTCTTACAGACCAGTCCCCAGTCTTTTCCAGTGTCCTGACTCTCTGAGAATCTCATGGAAGTTGTGTGTCTCCAGACAAAAGGCCGAGGTCTGCACTAGATTGGTGGACAGTCCCAGAGGGTCACGGGGGTGTGGGGGGTGGGAGTGCTGAAGCCAGCCTGCCTTCAGGGCCCACACTGAGAACAGCTCTGGGGAATCTAGAGGCTCCTTTGGTTTTGTTAAAGACGACGACGCAGAGCTGGGAGGACGGCTCGGCGAGGGAAAGGCCCTTTGCAGTCAAGCCTGATACCCTGAGTTCAATCCCCAGGACCCATAGGATAGGAGAGAACCCATTCCTGCAAGTTGTCCTCTTCCCCCTGCCCACATTCCACTATTGTTTTTGTGCATGCACACACACACACACACACACACACCAATGAAAAGACAGTCAATGCACTCCAAAGAAGTCTCCAACTCACAGCAGTCCTCTACCCCATCCCCCCACTCCTTTGCCAAGGGCTGTGATCACATGCACCCATCACGACACCCACTATCTGCTCCTTTTCATTTGAGGTAAAAATCCACATAAAATACAAATATCCATTTAAAAGCAGCCCACTTGGTATCATGTGGACAGTTTCCTGCAATGCGTCTAGAGGGGGAAAGGAGGTGGGGGGGAGGGGGGAGCACAGCAAGACGGCTCAGTCTTTAACGGAGACTGCTGCCAAGCCGGACAAACTGACTCTGATCCCTGGGACCCCTGTGGTGGCAGGAGAGACCCAACTCCCGCCAGCTGTCTTCTGACCTCCATATATGCCTGGTGACACAGTGCCCACTTACCTCCCACACACAAAATAAATAAATGGAATTACATTTTTTAGTTTAAAATTTAAAAAAAATTAAAACTCTCACTGCCCGTAGGCAGGACGCTCCCCCTCATCCCAGCTCTGGCAGCTGCTCATCCTCCATTATGGCACGTCCTGTGAACGGAGTCTGCTTTTTTGCTCTGGCTTCTCCCTCGCCACCATCCCCAAGTCCACCCCTGGTGAAGGCCATGCCTTTGCTCCATTCATTTCTGTGGTCCCATCACATCCCATTTTATGTATGGGGGGGGTTTATTTACTGAGACAGGGTCTCGCTGTGTAGCCCTGACTGTCCTGGAACTTGCTATGTAGACCAGGCTGGCCTTGAACTTGGAGATCCACCTGCCTCTGGGTTCTGGGATCAAAGCACTGTACCTGGTCCATGTGTTTTCTTCTTTCAATTTCGATTCTTTCTAAAAAAAAAAAAAAATTTTATTTATTTATTTATTTTATGTATATGAGTACACTGTAGGTGTACAGATGGTTGTGAGCCTTCATGTGGTTGCTGGGAATTGAACTCAGGATCTCTGCTTTCTCCAGCCCCACTTGCTCCTGCTCCAGGCCCCGCTCCCTCTGGCCCAAAGATTTATTTATTATTATATGTAAGTACACTGTAGCTGTCTTCAGACACCCCAGAAGAGGGCATCAGATCTCATTACGGATGGTTGTGAGCCACCATATGGTTTCTGGGATTTGAACTCAGGACCTCCAGAAGAGCAGTCAGTGCTCTTAACTGCTGAGCCATCTCACCAGCATCCTCAATTCTGATTTTATGTACACGGTTGTATTGCCTACATGTATGTCTGTGCACTGCATGTGTGCAGTGTCGTCAGAGGCCCAGAAGAAGATGCTGGATCCTCTGGAACTGGAGTTACCGAGGTTCGTGAGCTAGGACTTGAACCTGGGTCCTCTGGAAGAGAAACTCGAGCTCTTAACTGCTGAGCCCTCTCTCCAGCCCCAAATGTTTTTTGAATTAATTTGTAAGTACTGGGGATCGTGCTAGGCAAAGGTTTTGAGCCTCACCTCCAGCTGGGAGCTCACCCTTTAAAAAAGATTATTATTGTGTGTGTGTGCATGTTGTGTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTACCTATGGACATACAGGTACCACAATGCCTGTATGGAGATTGGGAGAACCCTGCTATCAGCTCCCTGCTGCCCCCTTGTGGCGCCAGAGAATTAGTTTAAGTCCTAAGTGCCTCTACCACCTACTGAGCCAACTTAAAGGGCCCTGCCTGGACGTTTTTGTTTTAAGAAAAAATACAAAGTGGAGACAGAAATCGAGAACTGGATCCAGAAATATGACATGGAGATGGGCGAGAAGCAGGTACGTGCCGGGGGTTCCTCCTGAGGGATTGGGGGAGGGAGGGTCGGGTAGGAGCTGCTCAAGGTGGCCTAGGTATGAGACTCCTCCCCTGGAGACCCTTTGAAAGAGAGAAGCTGAAACTGCTGAAATCCAGATTCATCCTGAAGCACGTGGCTCCACCTAATAGCCACCTCCCATGGTCCTCTCACAGCCCGGCTCAGCTCCTGCCTGCTCCCGGTTCACAGGCGAGCCCTCCTCCCCCACCCCCACATCACTCTGCACCCAAAAGGTCCAGGTGCCAGGCTCCCGAATAAAGCAGGGAATCGAGACGGTTCTCACTGTCGTCTGAAGGTTTCAAACTGGCATCGCTCATGAGCTGTGCCCCCTGATAGATCTGTAGATGCTGGTGGGAGGGAGGGATAGAGAGGAGGAGGAAGGAGGGCACAGAGTAGCTGGGAATAAGAGGAAAGGGAGAGGGAGAGGGAGAGAGCGGGGGGGGGGAGGGGGGGAGAGGAGGAGGAGGAGAAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGACGACTCGGACCAAGAAGAAGATATTTTTTAAGGGCCCACCCCACTACCCTTCCACGAACTACTTCCTCCCGGAAGGGCTCTCCTCCTGTCTGTTTAGCGATCAATGCATCCACTGGGTAACCCAGAGATGAGCTTGGAGCTTTCCCGATTCAGTCACTACTCAACAGCGCCACCTAGTGGGTACCAGGTCTCCAAACCAAGTGTCTGTGAAGGACCAGTTCATACTGAAACCCGAAGGTCTCTTGATCCCAGAAACTGGCCCTGGTATTTGGAATTGTCCCAGTGTTTCAAGTGAGGAAAACCAGAGCTCTCCTAAAGGTGGAGAGAAGCTAACAGATGGGATGCTAGCTCAGTAGGATGCTTGCCATGCAAACCTGAGGACCCAAGTTCGCATCCCCTGCATCCCATGTGAGAAGTGAAGGCGGCAATGTGCACCTGTGGTCACAGTGCTGGGGAGGGCACAGGCAGGCTGATCCTGGGAGTTTGCTGTCCAGCTAGCTCAGTCACATCAGCAAGCTCCAGGTTCAGTGAGAGAGCCTGGCCTCAGGAAGTAGGGTGGAGAGCCCGAGGAGGACACGTGACCTTGACCACCAGCCTCCACCTATAGTCACACACACACACACACACACACACACACACACACACACACACACACACACTGCATTTATTCACATGTGCATTTACACAAGAACAAAAGCACATAGGTTTGCACAACTCACCACCCTTGGTCCTCCCTCCTTTCTGAGATCTGCTCAGCCGGGTCTTTCCTGGAGCGAACCCAGGTATAAATATACAGACAGGCTCTGTATCCTACCTGGCTGGGCTGGAAGCCGGGCATGGGGAACGGGGAGGCCATGCCGGGTGCTGCGCACCTCCAGCCATGCCCCTCGCATAGGACGAATACGAGGACCTGGAGAGCATCCACAAGGAGGAGAAGCTGCAGCTGGAGGAGCTGAGGGAAAGGCACGCGGTGCTGGTGGAGGAGTTCTCTCAGATCCGGGCCGAGAGCGAGATCAATTCCAAGAAGCGGGTGGAGGCAGAGCGCGAGATGGTGCGCATGGTGAGAGCCGCCACGCTCATCCAGGCGGTGTGGAAGGGCTACCTGGTGCGCTCCATACTCAGGTCCAAGAAGAAGAAGCGGGGCAAGGGGAAGGGCAAAGACAAGGGGAAGGGCAAGGAGAAGCCCAAGGAAGAAAAGGCCAAGGAGAAGAAACCCAAGGCCAAGGGCAAAGGCAAGAAGAAGTGACCACATCCTGGTCTGGCCCCGCCCCTGTTCAGCTTCCACCACGCCCTACCGGGTCCCAACCAAGCCCCTGTTCTGTCCCAGCCCTTCCATCCCTGGCTTTAATGATGGCTAAATTAAAAAAGACAAACAACAAAACCCGTTTCAAACCACACTCAGTGGCTCACCCTTCAACAGACCTGTCTGTCCCAGGCTGAGGCTGAGGCCGTGGGCGCAACATCCTGAGGAGGGCGCAAACTTCGGATCCCCATCCTACCACACCTTCATTGAAAAATGAGGCCCTAGTCTTAGCTGAACTGTCAGGAGGGCAGCAGGGGGGTGGGGGGTGGGGGAGGGGTTGTGTGCTCAGACTGTTCCATGAGTTTTAAAAAGTACCAGTTGCTAGAATTTCACGAATTGAAAATTCTCCCATAGAAACTTAACAGCCCTTTCTGCAAGGATGTCAACACCCCTGACATCCCAGTAGTTGGGCCCTGTGTCTCTCCATGCAGCAGCAACCACTCTGCCAGCTTTATTTCCATGTAGCCCAGAGTGGCCTTGAACTTAAGGTGATCCTCCTGCTTCTGATTCCTAAATGCTGAAATCACAAGCACGCGCAGCTTTAACTAGCTGACTCCTTTCCACTCTGAAAAACAAAGTATGTGGGAGGGGGCAAGGGAGGGGGACCCTAGTGGCCCAGCCATGGCTTTAATCCCTTCAGTCAGGCAGAGGCAGGCAGATTTCTATGAGTTTAAGGCTAGCCTGGTCTACCAAGCTAGTTCTAGGACGGCCAGAGTTACATGGGGGCAAACCCTGTCTTGCAAAAAAAAAAAAAAAACAAAAACAAAAAACAGGAAAAAGGTTTATTTATGTGGATGTGCCTACAGAGGCCACAGCTGGGTGTGTAGTTCACTGGAGCTGGAGCTAAAAGCCTTGTGAACCACTTAAAGAGGGCGCCGAGATCTGAACTCAGGCCCTCTGACAGAGCAGTAACCCACGAGCCATCCCTTCCATTCCTACTTTGTTTTAATCTTAAAATGTGTGTATGTGTGCACATACCCATGTATGCCCATATATATATATATATAAAATCATGTATCATGTGTGCCTGGTGCCTGCAGAAGTCAGACGGTGTTGGCTCCCCTGGAATTGGAGTTACAGGCCTTCTGACGTGGGTGCTGGGAACCAAACCCAGGTCTTCTGGAAGAGCTGTAAGCACTCTGAGCTGCTGGTCCTCTCTCCAGTCTCCCCCCTTCCTCCCTCCACTTTTTGTGGTTCCTGTCCACAGCCAGACTCCAGGCCTGGTGTCAGCTAAGCTGGTGGCCTCACCCTGAGCTTCAGCTCTCCTTCAGTGTAGACGGAAGAATGTTTCTAGCCTGTGTCTTCTCAACATGGAAGGATGGGACAGACAAACAGGAGGGAAACCAACCAATTCTTCATGAAAGTTCTAGAAGCTTCAATACCCTACATGGCTAAAGCACAAAGCATGTCTGTGTCCAGGCATGGCTGGGGCACCTCTGCAGGTCATTATATATGTCCCTGGGCCTGGCTACCCCTAGACAGGGGTGAGATACCCACTGGGCTCTCTGGAATGTTCAGGGAGGGAGGGGGCAAGGAGAGGCTCAAGGTCCTAGGCCCATCATCCAAGGAAGGATGCAGGACCTTGGGTGAAGCTTGAGGCCCAGGGGCCCCTGCAGCCTGTGGGCAGTGCTGAGCACCCAGGGGTCTCTCTACTCGGCTCATCACTGAGCCCTGTTGTTTTGTTGGGCTGGGGGTTGCTGGTGACTGAATCCAGGTGCCATTCACATATGCTAGGCAAACACTCTACCACGAGCTATATCCCCGCTGCCCAACAAGACAATTGCTAGCCAAGGACTCT
GF3 -1 -1 1 CAGGCGACTTGGCACCTACCACACTGC
GF4 -1 -1 1 GCGTCCTGACACCACTGCATCATCCACAG
EX52 -1 -1 1 GTAAAGGTCTCAGTCGACCTCAATAG
EX32 -1 -1 1 GAAGGCGGCTGAGACACCGCTAGAGAGAC
GR3 -1 -1 1 CATCCTCCATTATGGCACGTCCTGTGAACG
GR4 -1 -1 1 GCAGCTGCTCATCCTCCATTATGGCACGTC
Comments:
Created
Target: 2803Ensembl ENSMUSG00000029600 -- 121059072 -- 121059971 ENSMUSE00000190599 -- ENSMUSE00000190599
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:5:1:152537259:1, start 121059071: end 121059971
Found 9 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:5:121065471:121066470_693 G3:5:121054572:121055571_556
ACCEPT (0) gap pair G5:5:121065471:121066470_693 G3:5:121054572:121055571_572
ACCEPT (0) gap pair G5:5:121065471:121066470_693 G3:5:121054572:121055571_235
ACCEPT (0) gap pair G5:5:121065471:121066470_698 G3:5:121054572:121055571_556
ACCEPT (0) gap pair G5:5:121065471:121066470_698 G3:5:121054572:121055571_572
ACCEPT (0) gap pair G5:5:121065471:121066470_698 G3:5:121054572:121055571_235
REJECT (1) gap pair G5:5:121065471:121066470_942 G3:5:121054572:121055571_556
REJECT (1) gap pair G5:5:121065471:121066470_942 G3:5:121054572:121055571_572
ACCEPT (0) gap pair G5:5:121065471:121066470_942 G3:5:121054572:121055571_235
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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