High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by dk3
Valid After Annotation
no
Comment Type Comment Detail Edited User Edited Date
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 113487
Chr: 11
Strand: 1
Status: Ordered
BACs: RP24-549D9 : RP24-398F8 : RP24-549N24 : RP24-194K22 : RP24-224B23 : RP24-205P9 : RP24-350P15 : RP24-206N19 : RP24-539A14 : RP24-539O16 :
Instance: Plate 116: Well D08: BACs RP24-398F8 RP24-549D9 RP24-549N24 RP24-194K22
Oligos on GRCm38:
Type Start End Length Seq
G5 115907350 115907399 50 ACAAGAAATCTAAGACTCCACCTGTCCTTGGCTCCTAAAAATTGGATTCA
U5 115912877 115912926 50 ATCTGGGAATTGGGACAATAAGAATAGGAGACCTACAGGTGTACACTTTA
U3 115913003 115913052 50 GCCCTCAGCAGCTTCTAAGCAGGAAATCCCATTTCCCACTTTTTTTTTTT
D5 115914084 115914133 50 TTAGTTCCTCAGCTGTCGAGGCTTTCCCACACATCCCCCTGGCTAGGATA
D3 115914163 115914212 50 CTACTTACTGCTTCCTTATCCCTCAAAATCCCAGTCCAAACCTGCCTTTT
G3 115918074 115918123 50 AAATAGATATCTGAGACAATACTGACTTTTTACTTTTAGGTTATTCATCC
Oligos:
Type Start End Length Seq
D5_D3 115775448 115775476 29 CTTTGCCTCCTCATACTCTCTTGGCTTGA
G5 115768664 115768713 50 ACAAGAAATCTAAGACTCCACCTGTCCTTGGCTCCTAAAAATTGGATTCA
U5 115774191 115774240 50 ATCTGGGAATTGGGACAATAAGAATAGGAGACCTACAGGTGTACACTTTA
U3 115774317 115774366 50 GCCCTCAGCAGCTTCTAAGCAGGAAATCCCATTTCCCACTTTTTTTTTTT
D5 115775398 115775447 50 TTAGTTCCTCAGCTGTCGAGGCTTTCCCACACATCCCCCTGGCTAGGATA
D3 115775477 115775526 50 CTACTTACTGCTTCCTTATCCCTCAAAATCCCAGTCCAAACCTGCCTTTT
G3 115779388 115779437 50 AAATAGATATCTGAGACAATACTGACTTTTTACTTTTAGGTTATTCATCC
G5_U5 115768664 115774240 5577 ACAAGAAATCTAAGACTCCACCTGTCCTTGGCTCCTAAAAATTGGATTCAGAGTTGAACAAGCCCTTACTTTAATCCCAGCATTTGGAGGTAGAGGCAGGTGGGCTTATGTCAGCTCCAATTGGCCAGGGCTATGTAAGACCTTTTCTCAAAAACAAAAACTCAGGAGTTGGGGATTTAGTTCAGTGGCAGAGTGCTCTGAAAAAACACAAACACAAACTACCACTGAATGATGATGGTAAAGAAACTAAAATGCATCGCGCTGCTTTTAAACCCTCTCTGTGGTGCTGCAAATGAAGCCCTGAAGACTGTGAGAACTCTCGGCAAGTGGTCTTCCACTGAGCCACACCCCCATTCATGAAGCCTACATTAGCCTCTCAAATGTTAGGATTACAGGAAATATGCCAGGTTGTCTTGAGTTTTTGCTTTGTTCCAATTGATTGATTGATTGATTTTCCAGATAGGATTTCTGTGTATATAGCCCTATCTGTCCCCGGCTACCCCAAAACTCACTCCATAGACCAGGCTGGTCTCAGACTCAGAGATCTGCCTGCCTCTACCTCCTGAGTGCTGGGACTAAAGGTATGTGCTACCACTGCAACCCCTGCTCTTTTTTTTTTTTTTGTTTTGTTTTTTTTTTTTTTGTTTTTTGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTAGAACTCACTTGTGTAGACCAGGCTGGCCTCGAACTCAGAAACCCACCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGTGTGCGCCACCACGCCCGGCGTAACCCCCTGCTTTTTTTTATTTTAAGGGACTGGAAACTATATTCATTACACTGGTGTTTTGCCTACATGTACATCTGAGTGAGGGTGTTGGATCCCCTGGGACTGAACTTACAGATAGTTGTCAAGTATCATGTAGGTGCTGGGAACTGAACCTGGGTCCTCTGGAAGAACAGTCAGTGCTCTTAACTGCCGAGCCATCTCTCCAGACCTTGTTGTTGTTTAGACAATCTCACTAAGTAGCCCTGGATGGAACTTGAGAAGCAAAATCCCTTGGCACCTAACTCCTGGCTGTCCTCTGCCTCTGTGGTGCTGGGATTACAGGCATTCTCCACAAGCCTGCTCCTGTCTTGAGCATTTTGATACAGATACGTTCATTTAACTATCATGATGCTTCAGGCCTATAGATGCCATTATTGCTCCATTGACAGACAGGGAAGTTGAGCCTGAGTGGCACGAGATAATTTGAGCAGCAAGCTGAAAAGTGGCAAAGCCGGCCTGCCTGGCTCCAGTGCACCTGCTCATCACACCTAGGGCTCGAGGCCTCTGGGACACAAGACATAAGATTCTGACTCCATTATCTCTATTAACCAACCCTACATTCAAACCCCCACACTCAAACTCTGCCCAAGACTGCTTCTGTGTGAGTGAGTGAGATTCCAGCACCCGAGGACCAGTACTGGGTTCAACCAGGCATCAGAAGAAAGTCCCAGAAACAGGGGAGGGTACTGGAAACCCCAAATTCTTCACTCCCAGTACTGTTGGCTGGAGGTCCCTGGAAGCCCTCAGTTCAGGGGTAGCAGCCCAGTTCCTTCCAGACTCTTCTTTCAGCAGATTTCATTAGCATCATGAATAGCCTTCCTGTAGGGTGGCTAAGACTGAAGCCTCTCCTTTGCTAGAAAACATTCTAGTTCCCAGTCTAGCTGCAGAGCAGTGGGTACCATGGACCACCCTGGACATAGCACAAGAAGCCAGTTCTCTTGCACATTCCTGCCCTGTGCTAGGACAGCCCATCCTTTAAGATAACCAATAGCAGCCTGACAAATTCAGGGGTGGACAGACTTCACCTAACTGTAGAGGAGGCAGAGGTCTTCCACGGTAGGAAACAGACAGTTGCTGCTGACCTGGGTTGTCAGAAGATTGCTGGTGACCACAGAAGAGGCACACAACAGACTACATCAAACCTAGGGAAGGTCCTAACTGTCACTGTCCTATGCCATTCAAGAGCAAGCCTCTTCAGTGCACAGTGGTCTCACCAGCTTCCCCATGGCCTGCCTTCTCACACAGCCTCTGTCCTAGCAGTGTCTGTTCTCAGTTCCTCGGCAGCATGAGATCTGCACATTTCATTGCCTGAATTTTATGCCTTATTGCAAAACTAAGAACCATGGCAGCAGCACTTAGCAGAGCAGATAAATTCTGTGACATATTAACACTTTCAAAGCAATTTATAACAAAAACCATCATACTAACACAAATGAGTATTACAAATTTCATATCATAAAATATGTGCACAGATTAATTTTGTCCTAGAAATATTCTCCTCTTCTTTGGGCCCTGGTTCTTGCCATGGAATGCTGTCCGTACCTTGTTTCTTTTTAAAATTTGTTTTTATATGTATCTTTGCCTACATTCATGTAAGCATCTATTTCCTCACAGTTACTGAGACAATAGATCTACTTGGGTACAGCAGTTATTCAAAACTAGACAAGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTTTGAAGGATCCAGGTTCAATTCCCAGCACCCATAGGACAGCTCACAACTGTAACATTAGTTCCAAGGAATACAACATCCTCACAGACATACATGCAGGCAAAGCACCCATGCACATACAATAGAAATAGAGTATTAAAAAGGCTAGACCAAGCAGCTCTCCTATGAATGACACATTTCTTTCCACAGACCTGATTTTGTTGCTGTTGTTCTGAGACAAGGTCTCAGTAGATATCTCTGGCTGACATGCCATTCACTCTGTAGACCAGGCTGGCCTCACACTCACACACCTGCCTCTGCCTCCTAGATGCTGGAATTAAAGGTGTGTGCTACCACACCTAGCTTGGATTCATTTTGTATTTTTAATGATTTATTAATCTTTTATATATATATGTATGAATGCTTGGTCCTTTCAGAGGCCAGACAAAGGTGTTGGGTCTCCTGAATAATTATAGGAAGCTGTGGCCACCTGGTGTGAGCGCTGAGCCCTGAACCAGGGTCTTTTGAAATGCAACAAGTGCCCCTAGCGTTGAACTGTCCCTCCAGCCCATGTACCTCTGATGTGGGCACAGGTGCCTCTGTGGGTGGGCTAGTCCTTCTGAGGAGGGGCTGCTGTCTAGCTCTGCTCGAGCCTTTTCCTTGTAGTTTTAATTTTATTAGAGCAAGAGTTTGAACACACCTGCAAAAGAAACTGAGTAGAAAATGTCACCAGCTGGGTGTGGTGGCGCACGCCTTTAATCAGAGCACTCGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCAAGACCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAAAAACCAAACCAAACCAAAACAAAAAAGAAAAGAAAAGAAAAGAAAGAAACAAAGAAAATGTCACCAGAGAGGGGCCAGCAAGATGGCTCAGTGGGTAAAGGAACTGGCCACCAAACCTTAAGACCTGAGTTCAATTTCTGGAACCTACTTCCACAAGTTGTCCACTACCCCTTCTCCATACACACAGAGGCCACGGCATACGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTATGAGCATGCGCACACACGCACAAAATGTATAAAAAAATTTAATGTCACGAGTTTTACAAAATGTGCCAAAGTTTAGTTATGTTTCTATTGTTTCGATGGGACTATGGAGTGATAGCAAAGTGGTCACATAGGTATTAATAGAGACAACAGCTGTCATCTGCTCTAAGAGCTGACTATACAGCTGGCTCCAAACTCCAACTGTACCAGCTGCACAACCGAAGTTGCTTAACCACACTGTCTGTCCTTGCTGTAAAATTGGAGTTGTGGTTGTCATTACTGCTTCATCTGTTGATACCATGTCAACGTTGTTATCATAGAAACAAGCTCAGCTGCTATACACCAGTCTGGGAACAAACAACTGACTCTTGATAAGAACCTCATTTAACTGATGGGCACAAACCATACCAGGGTGTGCCAGAGTCCCGCCTGCTGGCTGTGTGCATCTGGCAGGCCAGGAAGTCAGTGAGTTTTACACAAGGAAATGGCAGGCGACAACGCTTTCCTGAGCTTGTTCCTCACCTGTCACCACTCCAGGTTAGACCCCAGAGGATTGGATGACAGATACCCTGGCATCCCAGAAACTGTTATTCAGTGAGCATTTCCCAAACTCTGACGGCAGGATTGATGATGCACTAGAACGCAAGCCCTTCAGAGGCAAAGACCCACCTCTCTGATCTTACAACGGGCTATGGCAGGCCCACATGCCTAAGCTCTGAAAGGAGTCAACAGTGGCCGCCAAGTTCCGAGCAAGGCCAGTGTAATTAGTCCCCTTCCAAGGCGTGTGTGTGCACGTGCGTGCATGTGTGTATGTGTGTGTGTGTTGTGTGGTCGCCCCCTCTCCAGCTTCTTCTCTCTCTCTCTAACCACAGAACCAGCAGAGGCCATGCCAGAGGCAGGTACTGCGGGGAAAGGCTCCATGTGATAAGCTCTCCTACTCTTACCCAGAAACCTGTTCCAGGCAGTTCCTGCCAGAAGTGCAGTTAATCATTCACCCAAACCGCAGGAAGGAAGGAAAGAGTGCTCCCCTACGAGCTCTGAGAAGAGTGCTCCCAGGGAGATCTGAGAGCTCTGAATGGCTCTAAGGCCCAGCTCTCATCAGTGAGGCTGACAGGCGGCCTGGGGCACAGACCCACTCTCTCTTCTCACCCTAACTCAGGCTGACCTCTCTCAGGGACTTAAAACTCAACTCCTCCAATGCCATCACAGAAGGGTAGAATCCTAATGGAGGGAATGAGGGAGGGAGTGGCAGTTGCCAGGCAACCAGTCTAGCCCACCCTTGTTTGCTCTGGTGGTGGCAGCAAAGGTGTGGTCAGAGTATTTGGCAACTCCCTGGGCTGCCAACAGCAGTTTCTCTGAGTTGGGGCAAAGCTGTGGTCACCTGGAAGCCTCTTCCACCCAGGAACCCTGAAGTCTGAGGCCGGAAGGACAGCCTGACTTGCTTTGTCCTCAGAAGATTCAAGACTATTAACAGAAAACCAGGTAATGACATCTTTCTTCTTCTCCCACTCCCCAAACGGCTGCAGCCCCACACTCACCAGCTCCACTTCCGTAATATGGCAAGGCCTCCTGCTCCCAGCTGCATCCAAGGACACTCAGCCACGCTTTCTTCCTATCCACCACCAAGACTTTTTTAAGATTTATGTGTGTGCTGTTCTTACGTATGCATTTCCACACAGGCACTCCTGGTATTTTCAGAGGCTGGAGATGGTGTCAGGCGCCCTGGAACTGGCTTTGTGGAAGACTGTGAGCTGAGCAGCCAGGGCTGAAATAGGGTTTTCTGTTCTCAGCCGGCCTGGATCCCTGGGCTCAAGTAGGAATTCAGCCTTCTGTGGAGCTGAGACTTAAGTCACCATCACCCAGCTAGGCTTATAGACCAAGCTCTCAAAATGCCTCCCAAACATCTGGGAATTGGGACAATAAGAATAGGAGACCTACAGGTGTACACTTTA
U3_D5 115774317 115775447 1131 GCCCTCAGCAGCTTCTAAGCAGGAAATCCCATTTCCCACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGTCTCGAACTCAGAAATTCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGTAACCACCACACCCGGCTATTTCCCACTTTTTGATATTAGAGACTTTGAATCCAAAGACTCATCATCCCCGCAGGGTTGGCTAGCTAGCTGTGTCGAGCTGTCCAGAAGGTGTCATGGATAGCACCACTGGGCACAAGGACTTCAGAGCTCTCTGCACTCAGGGCCTCTTGTCTCCTCTCTTCCAGATTTCCATGGGCCAAATGGTACCACCAAGGAGCATCCAGAATGAAGACTTCTGGAAGAACCCGTGGGATGTGGGGGGCCTGACAGTGATTGGCTTGTTCACCTCCACGTTCCTGCTCTTCGTCTTATTTGCTGTTGTGTTTGGGTATGTGGAAAAGGCTGTGTTTGAAGAGGAGTGATCCGTCTCCTCCGGGTCCTAGAAGGCAGCAGAGAGAGAGGGGCTGATCTGCTCAGCCCCGACCAGCAGTGGGCTTCCATTCCCTCTGGCTCAAGAAAGAATCTCTGGGAACCATTGGACCTTGCCTAGGGGTTGGCCAGTTAGCCAGGTGGGAAGGCAGGTGTGGTACCATTTCTGGCTCTCTCACCCTGCACCAGCTGAACACTAAGCACACGGAGAAGAACTCTGCTCTGCCTCAGATGAGAGAGAGCAGCAGTGGCTAACCAGACCTTCTGATGAGAGTCCCTGCCCAAGCAGCTATTTTCAAGTCCTTGACAGAGAAGGACACGAAATGTCAAATGAAGTAAAACTTCCTTCTCAGATCCGTTATCGTGAGCTCATTTTCTACGCAGACATGTTCTTCTTTATGCTGGCTTCTCTAGCAACCTCACTAAGGCCTACAAGTCCTCGAGGGCTCTGACCCTGTGAACAGTAGACTCAGTCGCATGGGCTGTTTGACACGTGCCCCAGGCTCACCACAGGACCTTAGTTCCTCAGCTGTCGAGGCTTTCCCACACATCCCCCTGGCTAGGATA
D3_G3 115775477 115779437 3961 CTACTTACTGCTTCCTTATCCCTCAAAATCCCAGTCCAAACCTGCCTTTTCAAATCCATTCTCCCTGACTCTGACGCCAGAGACTGGGAAAGGGCATTCTGTTACTCTCAGAACAATACTCTACAGGGCTCGGGAGGCGGCTCACCAGCTAAGAGCACTGGCAGCTCTTGCAGAAGACCCTGGTTTGATCCATAGCACCTATGTGGTGGCTTACAACTATTTGAAACTCCAGATGCAGGGGAACTGATGCCTTTCTATGGCCTCTGTGGACACCAGGCATACATGTGGTGCTAAGATACACACTCAGGCAAAACATCCACATGCAAAATTTTTAAAAAGAAAAAAAAAAACTCAACCATGTACTTTTTCTTCAAAGTACTTTCATGAGAAATTTTATATAACTAATTATATATTAACTAACTATACATTTCCCAAGGAGACGAGCCCCATCGTCAGTGCCCTTGACAGAGCAGTAAAGACATAAGCACAAGAGTAGCTACACTGGACGATGCTGAGCAGCTAAGCTCCCTAGTGCAGAGCCCCCACAAGTCAGTTCTGCCCAGGCCAGCATGGAAATCTCCTCTTGCATCTTAAAATGCACACAGGTTACTTCAATGCCTAATAACTACAAGGTTAAAGTCCTGCCATGAATAGGTTTCTTTGTGGCTGCAGCTCAGAGAAAATGGCTACATGGAGGAGACAAACTAAAAACCACTGGCTGCCCCCCACCCTCCCGGCCTCAATTGGAATGGACCTGAATACCCTCTCTGGTGATCCCAGCCCAGTGCCCACATCCCACCCTATACATCCCTACAACAGCTTCCTTCCAGAAGTGTACCCACTTAGCTGAGCTTACCCCATTGGCTTTATCCCTCCCTGGCAGGTGCCTGCCTTAAGGGGTGCTGGTGTCACCCGACTCAGCCTCAAACCAGAACTATTCTTAGAAAGGCAACACCCTTCTGTTGAAGGGGCTTTCTTGCTTCATGTGTATACTGTCTACACAATGAACACTCTCTTCCATGTAAAGGACAGAGGGGACAGAGATGCCAGCAAGGTTTACTTCACTGGAGACCTGGGAATCCAGACCTCCAGACCCTCCCCTGTAGCCATCAGGATGGGAGGTGATGCATCACATGGACTTCTCGGCCCCACAGAAGCACACCCCCAACTGCCTACCAAGGCAATGAGAAGACCCAGAAGAAAGAAAGGCAGAGGCCTCAAGTTGGATCTGTTCTAAGGCATGGTAGGTTTTAGCACTCTGGTCTCAGACAAGAACCTACTAAGATTTTTTGCAGAGGAGACTGACAGCTGGAACATGCGGTTCTTTCATTCCTCACCATAAAGAGTTCTGGAGGAGGCTGAATTTAGTACATTCATGTACCAGTGGCCGGAGAGGGCTTTGGTGGTATGGTTTTAACCAAGGGGGGTGGGTAGCTGGCACAGCAACCCACAACTTTTTAGCATGTGATCTGACCACAGAGACCAAATTAGAGATGCCGTTTCCAAAAGGAATTACTTCAGTCAATGAATAGGAAGAAGTTGAGCATCCCAGTGTAGAACCAGATGAGCAGGACCTCTTCCCCCTTCACAGATAAGCAGAACCTCTTCCCCCTTCACAGGAGAGCCTAAGTAGACCTTGTGGACACGAGTTCTATGTAGTTGGTCATTGGCACTCGGCTTCAGGATGAGAATATCTGTGGTTGGAGTGTAAGTCAATGGTAGAGGGCTTGTATACTATGTGCGAGCTCCTGGTTCAATCCTTAACAGCACGAAAGCAGGCATCCCCTCTTCGCCCAAAGACGCTCCTCTTACAGAACCCAAGATAACCCATGTATGAGATGGCGTATCCAGCGTGACAATCAAGAAGCCCAAGTCAGTAGCTTCTGACATGAGATCTGTAACCATGACCCGCCTGGGCCAAAGGCCTCCTCAGCTCTCCGGATTTTTCCACAGAGAAGAAGGAATGTATTATTCCACCCACATTGACTCCTCTTGGGTATCATTCACACAGGGTTCTATCGACTACATGCCCAACACAGCCTATCATTGAACAGCTAACCGTCTCTCTCCATAGCTGGCCCATAGCTCCTTCCCCACTGCTACAAATGTATACAAATAGATAAGTGGATTCCACATAAGCAGGCTGGCATTTTGTTAGAAAAGCTGATTTAGCAAAGCATGCGCCACCCCGCCAACCACCAAAGATGAGTGGGCCCCAAGCTTCTGACTGGGCTCTGTATAAGAGAACAGCTCACCAGGTGTGGTGACGTATGCCTGTGATCCTAACACTTGGGAGGTGAAGGTAGTCAGGATAACCCGAAGTCAAAGGCCAGCCTAGGTTACAGGAGACTCCATCGCAAAAGCAAACAACCCAGCATGACCTGGTTCAAGTGTTCAGAAGAGGCAGCTCGATCGGTTAGAGCTGATCCCATTACTCTGCAGGGAACTGCTCATGGAGCCAGGCAGCCATGGAGTAGGCAGAGGAATGGATGACTATCCCACTGTGGAATAAGTCCTCACACACACAAGCCGGAGACTCATGGGGACTCAAGAAAAAAAAAAAAAAAAAAGGGAAGGGCCCTGACTTGGCAAGCAGGGGTCCCTTGGGCTAGCAAGGGCTGGCTTATGTCAGCTGAGAAAGTGAGGGTTGCTGGGAGAGGGCGTGGTCTGACACTATCCACAACCAGCATAAGTCATTGGCCTTTCCTCCCTGTGAGGAGCAGGGAGACAATGCTGGGATTAACCGCAGGGCCCTGCTCCTGCCAGACAAATATTCCACTAGTGAGTGGCTACGTCATATCCCTAAGCCCACTTACCCCACTTCTTCACAGAAGGTCACCAGGGCATCAAAGGCCAGCAGGGTGGCCTTATCAGACGGTTTGTTCCCCCTCTTTTCCTGGAGAGAGAAAAGGACCACTTAGTCCTCCAGAGTCATAGAAAGTGCTCTCACAGTCAGTAATGGAGAAGAAACGTGAAAGGACACCATTGAAGCCAGTTCCCAAGCGGGGATCTGGGCCTGACACTGGGTCAGCATCTCTGAACTACGAGTTGGACTTTCTCAATGGGAGGCGGCAAGAGCTGACATTTCCAAAAGGGTCTCAGGCTATTCTCATGCAAAGGGCCCTGGACTAACATTAGGGAAACTACGAACTTATCCAAGTTCTAAACAGTAAATACATGCACAGACTTTGGGTCCTTACAACGACATATATGTACATATATGCCAATGTGCATACCAGGAGATAGACTAAGCTGTTAACCCCTATCAGATTCAGATTATGCAGTTTTTGGTCTCTTTTATATTTTCCAAAAGTTCCTTTAAAAACTTAGCAATTTAGAGTCAGAGAGGAGCTTACTAGGTAAAAGTACTCGCTACTAAGCCTGAGGTTCTGAGTTCAAACCTCAGGACCCACATGGTGGAGGGAGAGAACTGACTCCACAGTTGTTCTCTGCCCCCCGCCCCCATGTGATCACATGCAATAATAAATGTAAGAAAAAATTCTCAAGAGATGCTGGAGAGATGGTTCAGTGGTTAAGAGCACTGGCTGCTCTTACAGAGGATCCGAGTTCTAGCTCCAGCACCCATGTGGTGACTCACAATCATTTGTAACCCCAGTTCCAGGAGATCCACTCCTCTCCTGACCTCCATGAGCACCAGGGACACATGTGGTGTACACACATACATGCAAGTAAATACTCATGAATACCAAATAAAATCTAAAAATATATATTTTTTTAAATACAGAAAATAAGCCAGGCAGTGGTGACACATGCCTTTAATCCCAGCACTCAGAAGGCAGGGGCAGGTGGATCTCTGAGTCTGCCTGGTCTACAGAGTGAGTTTCAGGACAAACAGACTCACACAGAGAAAACTGATCTCGGGCGGGGGTGGCGGGGAGAAGAAATACATAAAATAGATATCTGAGACAATACTGACTTTTTACTTTTAGGTTATTCATCC
U5_15 115759192 115774240 15049 ACCGTGGGGAAGGAGGAGAGGACAGGAGAGCCCAGCCTGTCTTTAGTACTTGTTATTTAGAGACAGGGTCTCACTCTGTAGCCAGGGCTGGCCCAGAGCTTACTGAGTACTCATCACAGTAGACTGGGATGGCCTCAGAACTCAGAAAGCCGCCTCTGCAACCATGCCTAGTCTTTTTTTGTTTGTTTTTAAGATAGGGTCTCACTACAGCTCAGGGTGGCCTTGAACTCATGAGCTTCCTGCTTCAGATTCCTGAGTTCTGGGTCTGTAGGCATGAGCTCCCACAAACAGTTTTCATGGGTATCTTGATCTTCCCTGAGGGAATTAGGCTTCCCCTGGCGCCCACCATGAGCTTTTTGCCCTAGGCTGAAGGCTGTACCTAGCATAGCTCCTGGAACAGATGATTCTCAACCTGTGGGTCTCAAGCCTTCTGGTGCTTGAAAGACCGTTCATAGGGTTTACCTAAGACTATCCACCAAAAAACATATATTACAATTTATAACTAGCAAAATTCCAGTAATAAAGAAGGAACAAAAAGGATGTTATGGTTGGGGGTCGCCAGAGCATGAGGAAGGCTGAGAGTCACTGTGCTAGAGGCACACAATGCTGAGAGCGGCCATCATGCCTAGTCACTCGTGAGCCCACTCACCAGGGAGATCCAGCCTCACTAGAGCCTCACTAGAGCCTCCCTCTCAGCCTCTAACTCCAGATGTTCCCCTGCGGATAACCACTTCCTGCCCAAGCTGGTGGCAGGAAGCCCACCTGGTGCAGGAGGCCAGTCTCTTTTCGGATCCCCAGGCTGAGGCCCTGGGAGAGGCCACTGTTCTATCAGGGTCCTTACAGTCATCTCTAGTGCCAGAAATGTGGTACAGGGTTGGGAACCAGAAGAGACAGCGCGAGGCAGGGCAGTTTGAGTGCCCAGGCTTAAGATTAGCCGGCACTAACAGCTTTGGAACAGAGGGTTTTCTTAGTCTGAATACTGCTTGCGGATCTGACCCCACACAGCAGGCTACTGTAAGGAAAGCAAATTGCAATGCCACCTTCTAAAGAAGTAGGGTCCCTCCAGCAAAGTGAAGAACAGCCCTAAAACTGCATAGAAGTGGCAGGAAGACAGCTGAGACAGCCACTGGGGGGCGCTCTTGCAGCCAGCTCCCACTAACACTGAATATACACGACAAATGTTCCCTCTGCCTGTACAGTGGTGGCCTGTCCTGTCTCTGAGAAGCTTTGGCATCTAGGCACTGTGACCATATGGTTGTCTACACCCTGCCCCAAGCCCTGGCTCACTGGGGTTTTGTGCTCAGATTCAAAGGGCTCCATTGACTGCAGAGCTGAGGTCAGTCCTCCCCCTGCAGCCTGGTCTGCTGGTGGCAAGACCGACAAACGTTAATGGGCCAACAGAGCTGTCATGCAGACAGCTGAGCAGAACGAGACCAGCAGTGACCTTTCTCTTAGAAGGACCCAAGGCCCCTCACTAAAACAAGCAGGCTACTTTTCTTTCTTTCTTTTTCTTATTTACTTATTTTTTGTTTTTTTCGAGACAGGGTTTCTCTGTGTAGCCGTGGCTGTACTGGAACTCACTCTGTAGACCAGGCTAGCCTCGAACTCAGAAATTTACCTGCCTCTGCCTCCCAAGTGCTGGAATTAAAGGTGTGCACTACCATGGCCGGCGCAGACTACTTTTAGAGTATCTAGGATCCTCATCTTCCCCCCAGATGCCCCTGGGTAGATGGCAGAGTACCACACCAGAGGGCTGCTTGAAAGCTTGCTGACGTGCTTGGGTGATCCTGTAGCCCGGTTTTCTTTCTGGCAGAGGGCAGAGAGAGCAGCCAGAGTGTGGCCCAAGAGACAGACCAGGGTGGTCAGGGACACAAGCCAAAAAGGCCATCCAGAGGGTGCTGGGGCACCCAAGAGCGAGTCACTCCACCCTCTTCCTCTGGCAACCTCAGACGGAGCCCCCCGGGGTTATTTCCACAGGGAACCACAGCCAACCACTTCTGTCATTAGCCCAACCCCAGCCCTCCGCCTACAGCCTACAGCCTACAGCCTGGCTCAAAGGCAGGAGGAGGGCAGAACAGGAGGAAGAATAAGAGAGGGAGCCGTCTGGGGATCCAGAGAGCAGAGTGGACCTCTAGGCAGGGAAAGGCATAGGGCGTGAAGGGCAAGTCAAGCTCAGGGGAGGACTCCCTGGGCTCCTCCTCACAACTGTAATTTGACACCTAGATCTCCAGGACAACCAACAACAAAAAAGCTGAAGCGATGAGGCGCTGAAGCTGGACAGCAGCTGGCTGTCAGCAACCGGGAGCTAGCACCAGGAGGTGCCCGAGTGAGAAGCAGCCGCCTAGGCAGGAAAGTGGCAGCGCTGGTGAAGAAGTCAAAGGTGGTGGCCAAAGGACTTCCAGGAGGTACATAGAGGCTCTGTACTGGGACACCTCTTCGAGGCTCCCCACTATCTGAGCTCCCAGGTAAGAGACACTGGCTAGAGGTGACAGGATCGGGAAAGGAGGGCAGCCAGGGTCTCGTATCTCTGGCGAGGAGTCACAGCTTAGCATGAGCTCGGAGAGGAACGTCTTGGGGGATATATTAGGCCACTCTCCTATGGGAATTTAGTCACCTCCTCTGTGTGTATACCTGAGTGCTCTTCCCCTGGGACCTGGTACTAGAGAAGGGGGATGTACTGCTACTCGGTGTGTGGCACAGGGGCTGGGGGCTGCTGGACACATCCATTTAAACCTTTTCTATTCAGTGAGTTTAGGATATGGCCCCCAATCTTGCTGAGCCTCCATTTCCGAAAGGATTAGGGCTGAGCCCTCCCTGGTTCTCAAGAAGGCTCAGGACAAGGTGTGCCCAGCCTGCTGAATGGCCTCTGGCCCCAGGAGCTGGTAGAGCCAGCAGCAGAACTGCCTACTTGGATGCTGGACAAGAGCAGCAGCTTTCCTGTCTTTTGGAGTTCTGACCTGCTCACCGCTGTGTGCTTCCGGCTCAGGATCATCCTAGGGAGGAGTCTCTGCACCCACTTAGATTACCAGGACCCCTGATCCTCCTTTGGCAATGATCTCTGAAAACTGAGCCAGGGATATGCACCCTAATTCAAACCCCAAGTTTGGAAAGGAGTTTGTGACTACATGAATGAGTTTGGAAAAGACCCCAGAGTCTCTTCCACACTAACTACTGTAATGGTTGCCCCCAGACAGCTCTTCTCTTCAGTCCTTGTGCAGGGCAAGCACAACCTTCTTGGGCAGGGCCTCACGAGAGGAGGCTGCTAAGCCCTTCTGCCTCCTGTCCTCACACTGGGGTTCAAGTCCCCGTGAGACGTCCCTTCCAAAGCAAACACACTGTTATCCAGTAAGAAAGAAAAACATCATTCAGTTTTGGAAACCAAGGGGAGGGCCGAGAGTCTCTTTGGCTCAGCAGAGGGGGTGCACTCTGGTTTGTGTGGGACTGGGAAGCTAACTCCTGTGGTAATACTAACAAGTGTGCAACAGAGGGACTGCACTGCAGCTGAGCCACATCTGGGACACACAGGCTCCAGCAGTCCCTGAGCTCAGAGGGAGGCCCAGCTTCTATGGCTGCAGGAGAGGAGCTCGCTTAGTTTCTATTGGCCACCAGAGCAGGAAGTTCTCTCCATTGTCTAGAAAACTTGGGGACCAGGGCAGCAGAACCACCAAGGCTTCAAGCACCCACTTTTGACTCCAAACCGGAGCCCCAGCACTGATGGAGTTGCTCAAACACTCAACAGGCACAGTGGGCATCTCTGCCCTGCTGGTTAGGCTCTGACCTCTCAGGTCTCTCACATCTGTTGGTTATGTCTCATTCGAGATGCTCCTCAACGGACAAAAGGGGCTGAGAGCCCCCATGACTCTTATCAGGCACAGACACGCTGCTTGCCCTCATCCGTGTGGGGAGCTGGAGTTGGATGATCAAGTAGACAAGGAGGAAGTAGAGAAACTGTGGGACCAAGCTTCTGCTGTCTGGCCTACAGGACACTTACTATATAGCAAGAACCTCCATGTCCCATGCCACACCCAGGAACACTAATCGGTCCCCCAGCTCGGCCAGTTTCCTTCTCTGTTGTGCTAGCTCTCTGATCACTGCCTGTGGAGCATCCACAGAAGGTTCCAAATCTGCTGCACAAGCGGCCTGGGCACCAGTGGGCCTCAGTGGCAGCCAAGCCTGTTCTCCCTCCTGGACCAGAGAAAGCTACCATTTGTTCTACCAAACATAGCATTAAGAGAGACTCTAGTTCCCCCAGGTTTGGGGCATAAAGTCTGGTTTAGAAGTAATTGGACAAACCTGGGACTGATCGTTTTGGAAACACAAAATTACCAGTAGCTTTGCTTGGCTTCACCCAAACCTAGGGTGTATCCAGTCAATGGGGCCCAGCTCCAGGGCTCCCTCTCCCCCTGACCACGGCTGGGGAGCAAGGTTATATTGGGCACCGAGTGTCTGTTGGCTCTGCTTTAAATAGCCCACTGGGAAGATGCCTGGAGACCTGCTGGAATGTGCCTCTTTCCTCTTTGGAGAACAAGACCCCTTTGCTCCCCCCTCTGAGATGTGCTTGTTATGTTGGGTCAGAGCTTAAAATAACAAAACACTAACTGCCAAGCAAAGAGGGTTCCTCTTCAGCCCTTTGAGGGCAGAGAGCCATACTCTGCAGCCCCGTTTCTGCTGGTAACACAGACAAGAGCTAACGCCCCAGGTCACATCCCAAGGCTGGCATAGGTGGCCACGTTCCACCGAGCTTCCAGTGGGGATGACTATAGAAGCCCTGGGCACAAAGTTATGAGGCTGCTCACAATAGGTGGTCCACGTTCTTACAGAGAAGAGCTCAGGTGCCCGGGGCACAGGTCTGCAGTTCTCCAACTCCACAAGCCATGCATAATGCAGAGAGGAGTGGGGAAAGGCACAAGGGAAAACACGAGAGAAGCTGGGAGTAACTGACCAACAAAGCCTGACTGCCCTGCATAGCAGGCCCAGCCCCAGCCTCCTGCCTTCTTCCTGCTCTGGGAATTGACACTGCTAGACCCTTCAGCTAGGCCTAGGGTCCCAACCCCACCAACCCTGACAGAGCTGGTGCTTCTTCCCCCCCTAACCGCCATTTCTCTTTAGGTCTTAGGGTGTGGCACACAAGTGAGAAAGCTCTCCTGACCACCAACTCCTGCCTGCTTCTGCTTTTAAGGAGTGTCTCCATTGGGCTTGTGAGGCAATGTGGGCACAGTGCCAGTCAGACTGAACAAGCCAGACCAAACATAGATGAAGAGGGAGGGTAAGAAACAACAGCCCTGGTTCTGAACCTTATGCACACTATTTCTGGGGGGGGGGGGGGGGGGGCAGGAGTGTTACAGAGGTCCCAGGGTAGGAGGGCGAATACATCACTGTCCTTCTGCAAGCATGTTCCAACTGGCTAGGGGCAGCATCTCCTTTGTTCTCACCAGAAGGAACGGTCTTAGGTTCATAACAGCAGGACACACAGATCTGTGCCTTTAGTGACAACACATGCAGTTCTCATAAAGCCTGGTGTGCCCCACTCTGGTGGTGATGAACTGATAAAAACCGATGCCCCAGGCCCACAAAAGGCAGGCGGTATTGACTGTGCTCAGCCCATGGGCTTCCAAATAGCTACAGCCTGGTTCTTGCACAAAGTCAGGAAGGCCTCTATCAAGAGCATATGGGCAGCAGGAGGGGCACTGGCTAGGCCCATCCTTGTCTTTCCTGGCACTTGTCAGGCCCCACCCAGAGAGAGTTCCAGGAGCCAGGCCCCTCCCTAGGGCTTCTCCTCTCCACTACAGCAGAGATAAAGAGCCTGTCCATGCACAGGAGGGGGAGGGGTTGAGACAGCAGGATCGTGGCAGTTCTACGAGGGCAGAAGTTAGAGGGTACCACATGACTGGAGTGTAGCTCCACGGCACAGTATTTGCCTGGTTTAAGAAAGGTCCCAGAGTCCATTCCCCAGTAGCACGAAAAGGAAAACTAAACACACAACAAACTCAGAGAAAGCACCATGTGCTTTACAGAAAGGAAGTCCAAATGTTTGGCAGCTGTGGTCCAGTTCCTGAAGAACTGTTAGGGAAGAAGGGCAGCAGCCGGGGCCTACTCGACTAAGGAGACGTGGAGGAGGTCCGAAAGGGCCTGGCCTTCTACACTGGGCAGCTACAGGCAACCAGGCCTCTCACTTTCAGAGGCTGCACAACACACAGGCAGAGAGCACACGTGTAGGATGACCCAATCACATGACCTTCAGAGTAGTTTTAGACCAGCTATGTGCCAGGCAGGAGTAATGGGAGTGACCAGTGTCACACATCACAGCAGAAGGAACCACCTGCTTGGTTGAGACCAGGGCCAAGGTAGCAATCATTGAAGACTGGTCACCTGCCTCCATCCCTCAGGAAGAACCAGTTTGGACACACTGGCACAAGGTTGTCCCTGTTCTCACTCACAGGACAGATGTAAGGGGAGGAGGCACACCTTCAAAGCAAACTCTGCTGAGAACTTTGCCTAGGCTCTCTCCAGGAGCCCTGGACCGTCACCACTCTACACACAGGTCTTTTCTGGTTACAACTGAACAGGTCCAGGCCAGGAGGGGAAGAGTTGGTTGAGCCAGTCCTAGAACTTCCCCTCCCATCTAGATAAATGAGGCCTCCAGGGGATACCGCGTGGTCCCTTCTTGCTTACACGACAGTAATATGAGTGAAGACACTGGCCTGGGAGGAGAAGGGGGGTTTCTTGGGGACTCAGCTTCATATCATTCAAGCACACAACCCCTCACTCTACAGCTGATTCCAGCTAGAGTCCTCATGAACCTGTTCTTGGAGTCTGATTCTCCTCCTACACAGAATCCTAAGACCTGTCTTGTTCCCCGGAGGGCATCTAGGGGAGGCACCTGAAAAACATCAGAGGCGGGCCAGGAAGCTGTGTCAGCTGAGGCACCCCAGCAAAACCTGGTGCTGCTGTCTCAAGACAAAGGAATGCAACAGTATAATCTGACCATCTGATGCTCAGAACCTTACCTGACAGCCCCTTAGAGGCTCTCTCCAGGTCACAATTATCACAAGGTTCCCTTGCCGGGGCGGCTCCAGGCAGGTGTGGGCTGAGGGCTGCCACTGAGCAGACTTCCATGACTCCCTGTCTCCCGCTGCGCTGCCTCGGATGCACCCGTGGTACAGGGCTGAAACAGACAAACCTGTTCTGCAGGCTATGGTGGGAGAGGTTGTTCACTGGAGCTACCTCCCAGCCAGCGAGCAGGGACTCTAGGCTTCTCACTGTCTCTGTTGTTGCAGACCGGCAGGAGCCCTACCACCATGGCAGCCACAGACTTTGTGGGGGAGATTCGCTCTGTGGGAGAGAGGCTGCTGCTGAAACTGCAACAACTCCCCCAGGCGGAGCCCGTGGAGCTCGTGGCTTTCTCCATCATTGTTCTTTTCACAGGCAAGTGGGGCATCTGTCCTGACCCTTAGCTCTGTCCCTTAGTGTCCCAATACCTGGGAGACCAACCATGGTGCCAGCTGGAAGGCTGGCGAGTCTCCTTCAAAGAAGTAGGAAAAGCAGTGTCCCAGAGGAAGCTAAGGGATTTGCCATATCCTCTGACACTGCCTAAGTTGCTAGGCAGTGCTGGGGACTGGAAACCACCAGGTGTCAGCCATGAACTGAAAGGAGTCAAAAAGAGTCACGAGGTAGTGGAGCGTGACAACAGGCCTCTCAGCCTCTCCAGTCCTTCAGGGGTGGGGAGGAGGGAGGGTCTCTAGAAGGAGGGTGGCCATCACTTCTGTCCCTGGCCTTTCAGCTCTGGGCCTTTCTCTCCCTTCAGCTACTGTCCTGGTGCTGGGGCTGATAGCCTGCAGCTGCTGCTGTGCTCACTGCTGCTGCTCTGAGAGCAGACAGCGGAAGATCCCGGTGCGGCCCACAAAACCCAGATGAGGGACAACAGCGAGCTGCCTGGAGAGAAGCTGGACAAGAGCCTTCCAGGACTGTAACCCAGGCTATTACCATGGCCACTTTACTCTTCTAGACAAAGGTTACCTGGCCCCAGTTGTGGCATTTCCCAGGTGACAGGACAGACATCTGGAGTCTTATCAAGGAAACAAGTGTTGCCCCATCTGCCGGGGACACGGGGCTCTGTTGGAGCTGATGGTTTCACACTCAGACCACCACCTGGGGCATCTGCTAAGCTGACCCTTTTAAGGGTCCCGCACTTCAATAGGGACCAGCTCATGCTTGTCAGGAGCTGGCTCCTGAGGCTCTGCTGACCTATGCCAAAGAGAAGTAAGCTAGCGAGGGACTCTGTGCACTTTATCAGACTCCAGGCCTCCTGCTGAGGGGGAGGAACATGGCAGCATTCTGGTTAAAATTTGTCTGCTTATTTCCAAGTAAACACTTTTTGGTTATGTGTTTATGAGGTTTGGCTGGGCTCTGTGTATCGGAAGGGCAGTGCCAGAATTCCAGGGGCATGGCTGTTGACTGTTCTTTCCGGGCTACTGCTCTTTGTGTGACCATGAAAGCTGAAAAATCACACAGGAGGAATGCCACCAGGGCATCAAGGCCATGGCACCAGAGGAGAGCCACAGCAACATTCCTGTTTTGCATCCTCTCTCACATGCTGGCTTCCAGACCCCTACTCTGTTGAGTGATGGAGGCTGTTGCCCCGGGACAGGTGGTAGGCCCAGGAATGCCTCTTTACATTTCCGCTGTCCTAACTCTGTCTGTATACCTCACACTAGCAGCCTCAGGTCCCTGGATCCTCCCACATTTCTGCAGTTGAAGAAAGGTTAGGCTCTTAGTTAGTTAGGTCTTTCACACCCGCCCCTTGATGTTTTGGTCCTGTTCCTATTTGTGTGCTGTCTGGACTCTGCTCACACACCCTCAGCTGACACCTGCCAGCTTCGTGACCCAATCCTCTTAAGCTAGGCTTCCTTCTGGTGAGATGATTAAGTGTGGCACTTGCTTCCTGGGATATCCAGCAAGACAGCCCAAGTCTAACAGTGCCGGGCACACTCTAGAACTCCGTAATCATGAACTGTGCTCATCTGCCCTCCTAAGCAGGCCTGACCAGAGCACCAGGGCCCATTTTAAAGAACAGTGCTGCCGAGATGCTGTCATGCCGCCTCCCCCTCTCCCACCAGCAGGTTTCTTTCTTCCAGATACACAGCTACAAAACTCAACGTGGAGGAGGGGGACAGACATGTGAGTCTCCATACATGTCAACAGGACCAGGACTTGGCTCTCTAGCCATCAAAGGCTCTAATTACTCACTCTGACCCCTCAGTCTCTGCAGCACACGTTCTGCTTGCTTGGGAGCAGAGCCCACCTACCTTGAAGACAGGTCACAGATAGGGAAAGTCAGTCCCTGCCACCTCTAGCTGGCAATCCAAACCATACAGGCAGCCAGTTCTCACTGGCTGGTCTGACTATCTGCTGTCTACAGAGTTGGCTGACAGGTTTGGGTTCTTGGAGAACCAGAAATAGTTTCCAAACAAAGACAAGAAATCTAAGACTCCACCTGTCCTTGGCTCCTAAAAATTGGATTCAGAGTTGAACAAGCCCTTACTTTAATCCCAGCATTTGGAGGTAGAGGCAGGTGGGCTTATGTCAGCTCCAATTGGCCAGGGCTATGTAAGACCTTTTCTCAAAAACAAAAACTCAGGAGTTGGGGATTTAGTTCAGTGGCAGAGTGCTCTGAAAAAACACAAACACAAACTACCACTGAATGATGATGGTAAAGAAACTAAAATGCATCGCGCTGCTTTTAAACCCTCTCTGTGGTGCTGCAAATGAAGCCCTGAAGACTGTGAGAACTCTCGGCAAGTGGTCTTCCACTGAGCCACACCCCCATTCATGAAGCCTACATTAGCCTCTCAAATGTTAGGATTACAGGAAATATGCCAGGTTGTCTTGAGTTTTTGCTTTGTTCCAATTGATTGATTGATTGATTTTCCAGATAGGATTTCTGTGTATATAGCCCTATCTGTCCCCGGCTACCCCAAAACTCACTCCATAGACCAGGCTGGTCTCAGACTCAGAGATCTGCCTGCCTCTACCTCCTGAGTGCTGGGACTAAAGGTATGTGCTACCACTGCAACCCCTGCTCTTTTTTTTTTTTTTGTTTTGTTTTTTTTTTTTTTGTTTTTTGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTAGAACTCACTTGTGTAGACCAGGCTGGCCTCGAACTCAGAAACCCACCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGTGTGCGCCACCACGCCCGGCGTAACCCCCTGCTTTTTTTTATTTTAAGGGACTGGAAACTATATTCATTACACTGGTGTTTTGCCTACATGTACATCTGAGTGAGGGTGTTGGATCCCCTGGGACTGAACTTACAGATAGTTGTCAAGTATCATGTAGGTGCTGGGAACTGAACCTGGGTCCTCTGGAAGAACAGTCAGTGCTCTTAACTGCCGAGCCATCTCTCCAGACCTTGTTGTTGTTTAGACAATCTCACTAAGTAGCCCTGGATGGAACTTGAGAAGCAAAATCCCTTGGCACCTAACTCCTGGCTGTCCTCTGCCTCTGTGGTGCTGGGATTACAGGCATTCTCCACAAGCCTGCTCCTGTCTTGAGCATTTTGATACAGATACGTTCATTTAACTATCATGATGCTTCAGGCCTATAGATGCCATTATTGCTCCATTGACAGACAGGGAAGTTGAGCCTGAGTGGCACGAGATAATTTGAGCAGCAAGCTGAAAAGTGGCAAAGCCGGCCTGCCTGGCTCCAGTGCACCTGCTCATCACACCTAGGGCTCGAGGCCTCTGGGACACAAGACATAAGATTCTGACTCCATTATCTCTATTAACCAACCCTACATTCAAACCCCCACACTCAAACTCTGCCCAAGACTGCTTCTGTGTGAGTGAGTGAGATTCCAGCACCCGAGGACCAGTACTGGGTTCAACCAGGCATCAGAAGAAAGTCCCAGAAACAGGGGAGGGTACTGGAAACCCCAAATTCTTCACTCCCAGTACTGTTGGCTGGAGGTCCCTGGAAGCCCTCAGTTCAGGGGTAGCAGCCCAGTTCCTTCCAGACTCTTCTTTCAGCAGATTTCATTAGCATCATGAATAGCCTTCCTGTAGGGTGGCTAAGACTGAAGCCTCTCCTTTGCTAGAAAACATTCTAGTTCCCAGTCTAGCTGCAGAGCAGTGGGTACCATGGACCACCCTGGACATAGCACAAGAAGCCAGTTCTCTTGCACATTCCTGCCCTGTGCTAGGACAGCCCATCCTTTAAGATAACCAATAGCAGCCTGACAAATTCAGGGGTGGACAGACTTCACCTAACTGTAGAGGAGGCAGAGGTCTTCCACGGTAGGAAACAGACAGTTGCTGCTGACCTGGGTTGTCAGAAGATTGCTGGTGACCACAGAAGAGGCACACAACAGACTACATCAAACCTAGGGAAGGTCCTAACTGTCACTGTCCTATGCCATTCAAGAGCAAGCCTCTTCAGTGCACAGTGGTCTCACCAGCTTCCCCATGGCCTGCCTTCTCACACAGCCTCTGTCCTAGCAGTGTCTGTTCTCAGTTCCTCGGCAGCATGAGATCTGCACATTTCATTGCCTGAATTTTATGCCTTATTGCAAAACTAAGAACCATGGCAGCAGCACTTAGCAGAGCAGATAAATTCTGTGACATATTAACACTTTCAAAGCAATTTATAACAAAAACCATCATACTAACACAAATGAGTATTACAAATTTCATATCATAAAATATGTGCACAGATTAATTTTGTCCTAGAAATATTCTCCTCTTCTTTGGGCCCTGGTTCTTGCCATGGAATGCTGTCCGTACCTTGTTTCTTTTTAAAATTTGTTTTTATATGTATCTTTGCCTACATTCATGTAAGCATCTATTTCCTCACAGTTACTGAGACAATAGATCTACTTGGGTACAGCAGTTATTCAAAACTAGACAAGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTCTTTTGAAGGATCCAGGTTCAATTCCCAGCACCCATAGGACAGCTCACAACTGTAACATTAGTTCCAAGGAATACAACATCCTCACAGACATACATGCAGGCAAAGCACCCATGCACATACAATAGAAATAGAGTATTAAAAAGGCTAGACCAAGCAGCTCTCCTATGAATGACACATTTCTTTCCACAGACCTGATTTTGTTGCTGTTGTTCTGAGACAAGGTCTCAGTAGATATCTCTGGCTGACATGCCATTCACTCTGTAGACCAGGCTGGCCTCACACTCACACACCTGCCTCTGCCTCCTAGATGCTGGAATTAAAGGTGTGTGCTACCACACCTAGCTTGGATTCATTTTGTATTTTTAATGATTTATTAATCTTTTATATATATATGTATGAATGCTTGGTCCTTTCAGAGGCCAGACAAAGGTGTTGGGTCTCCTGAATAATTATAGGAAGCTGTGGCCACCTGGTGTGAGCGCTGAGCCCTGAACCAGGGTCTTTTGAAATGCAACAAGTGCCCCTAGCGTTGAACTGTCCCTCCAGCCCATGTACCTCTGATGTGGGCACAGGTGCCTCTGTGGGTGGGCTAGTCCTTCTGAGGAGGGGCTGCTGTCTAGCTCTGCTCGAGCCTTTTCCTTGTAGTTTTAATTTTATTAGAGCAAGAGTTTGAACACACCTGCAAAAGAAACTGAGTAGAAAATGTCACCAGCTGGGTGTGGTGGCGCACGCCTTTAATCAGAGCACTCGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCAAGACCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAAAAACCAAACCAAACCAAAACAAAAAAGAAAAGAAAAGAAAAGAAAGAAACAAAGAAAATGTCACCAGAGAGGGGCCAGCAAGATGGCTCAGTGGGTAAAGGAACTGGCCACCAAACCTTAAGACCTGAGTTCAATTTCTGGAACCTACTTCCACAAGTTGTCCACTACCCCTTCTCCATACACACAGAGGCCACGGCATACGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTATGAGCATGCGCACACACGCACAAAATGTATAAAAAAATTTAATGTCACGAGTTTTACAAAATGTGCCAAAGTTTAGTTATGTTTCTATTGTTTCGATGGGACTATGGAGTGATAGCAAAGTGGTCACATAGGTATTAATAGAGACAACAGCTGTCATCTGCTCTAAGAGCTGACTATACAGCTGGCTCCAAACTCCAACTGTACCAGCTGCACAACCGAAGTTGCTTAACCACACTGTCTGTCCTTGCTGTAAAATTGGAGTTGTGGTTGTCATTACTGCTTCATCTGTTGATACCATGTCAACGTTGTTATCATAGAAACAAGCTCAGCTGCTATACACCAGTCTGGGAACAAACAACTGACTCTTGATAAGAACCTCATTTAACTGATGGGCACAAACCATACCAGGGTGTGCCAGAGTCCCGCCTGCTGGCTGTGTGCATCTGGCAGGCCAGGAAGTCAGTGAGTTTTACACAAGGAAATGGCAGGCGACAACGCTTTCCTGAGCTTGTTCCTCACCTGTCACCACTCCAGGTTAGACCCCAGAGGATTGGATGACAGATACCCTGGCATCCCAGAAACTGTTATTCAGTGAGCATTTCCCAAACTCTGACGGCAGGATTGATGATGCACTAGAACGCAAGCCCTTCAGAGGCAAAGACCCACCTCTCTGATCTTACAACGGGCTATGGCAGGCCCACATGCCTAAGCTCTGAAAGGAGTCAACAGTGGCCGCCAAGTTCCGAGCAAGGCCAGTGTAATTAGTCCCCTTCCAAGGCGTGTGTGTGCACGTGCGTGCATGTGTGTATGTGTGTGTGTGTTGTGTGGTCGCCCCCTCTCCAGCTTCTTCTCTCTCTCTCTAACCACAGAACCAGCAGAGGCCATGCCAGAGGCAGGTACTGCGGGGAAAGGCTCCATGTGATAAGCTCTCCTACTCTTACCCAGAAACCTGTTCCAGGCAGTTCCTGCCAGAAGTGCAGTTAATCATTCACCCAAACCGCAGGAAGGAAGGAAAGAGTGCTCCCCTACGAGCTCTGAGAAGAGTGCTCCCAGGGAGATCTGAGAGCTCTGAATGGCTCTAAGGCCCAGCTCTCATCAGTGAGGCTGACAGGCGGCCTGGGGCACAGACCCACTCTCTCTTCTCACCCTAACTCAGGCTGACCTCTCTCAGGGACTTAAAACTCAACTCCTCCAATGCCATCACAGAAGGGTAGAATCCTAATGGAGGGAATGAGGGAGGGAGTGGCAGTTGCCAGGCAACCAGTCTAGCCCACCCTTGTTTGCTCTGGTGGTGGCAGCAAAGGTGTGGTCAGAGTATTTGGCAACTCCCTGGGCTGCCAACAGCAGTTTCTCTGAGTTGGGGCAAAGCTGTGGTCACCTGGAAGCCTCTTCCACCCAGGAACCCTGAAGTCTGAGGCCGGAAGGACAGCCTGACTTGCTTTGTCCTCAGAAGATTCAAGACTATTAACAGAAAACCAGGTAATGACATCTTTCTTCTTCTCCCACTCCCCAAACGGCTGCAGCCCCACACTCACCAGCTCCACTTCCGTAATATGGCAAGGCCTCCTGCTCCCAGCTGCATCCAAGGACACTCAGCCACGCTTTCTTCCTATCCACCACCAAGACTTTTTTAAGATTTATGTGTGTGCTGTTCTTACGTATGCATTTCCACACAGGCACTCCTGGTATTTTCAGAGGCTGGAGATGGTGTCAGGCGCCCTGGAACTGGCTTTGTGGAAGACTGTGAGCTGAGCAGCCAGGGCTGAAATAGGGTTTTCTGTTCTCAGCCGGCCTGGATCCCTGGGCTCAAGTAGGAATTCAGCCTTCTGTGGAGCTGAGACTTAAGTCACCATCACCCAGCTAGGCTTATAGACCAAGCTCTCAAAATGCCTCCCAAACATCTGGGAATTGGGACAATAAGAATAGGAGACCTACAGGTGTACACTTTA
D3_15 115775477 115790527 15051 CTACTTACTGCTTCCTTATCCCTCAAAATCCCAGTCCAAACCTGCCTTTTCAAATCCATTCTCCCTGACTCTGACGCCAGAGACTGGGAAAGGGCATTCTGTTACTCTCAGAACAATACTCTACAGGGCTCGGGAGGCGGCTCACCAGCTAAGAGCACTGGCAGCTCTTGCAGAAGACCCTGGTTTGATCCATAGCACCTATGTGGTGGCTTACAACTATTTGAAACTCCAGATGCAGGGGAACTGATGCCTTTCTATGGCCTCTGTGGACACCAGGCATACATGTGGTGCTAAGATACACACTCAGGCAAAACATCCACATGCAAAATTTTTAAAAAGAAAAAAAAAAACTCAACCATGTACTTTTTCTTCAAAGTACTTTCATGAGAAATTTTATATAACTAATTATATATTAACTAACTATACATTTCCCAAGGAGACGAGCCCCATCGTCAGTGCCCTTGACAGAGCAGTAAAGACATAAGCACAAGAGTAGCTACACTGGACGATGCTGAGCAGCTAAGCTCCCTAGTGCAGAGCCCCCACAAGTCAGTTCTGCCCAGGCCAGCATGGAAATCTCCTCTTGCATCTTAAAATGCACACAGGTTACTTCAATGCCTAATAACTACAAGGTTAAAGTCCTGCCATGAATAGGTTTCTTTGTGGCTGCAGCTCAGAGAAAATGGCTACATGGAGGAGACAAACTAAAAACCACTGGCTGCCCCCCACCCTCCCGGCCTCAATTGGAATGGACCTGAATACCCTCTCTGGTGATCCCAGCCCAGTGCCCACATCCCACCCTATACATCCCTACAACAGCTTCCTTCCAGAAGTGTACCCACTTAGCTGAGCTTACCCCATTGGCTTTATCCCTCCCTGGCAGGTGCCTGCCTTAAGGGGTGCTGGTGTCACCCGACTCAGCCTCAAACCAGAACTATTCTTAGAAAGGCAACACCCTTCTGTTGAAGGGGCTTTCTTGCTTCATGTGTATACTGTCTACACAATGAACACTCTCTTCCATGTAAAGGACAGAGGGGACAGAGATGCCAGCAAGGTTTACTTCACTGGAGACCTGGGAATCCAGACCTCCAGACCCTCCCCTGTAGCCATCAGGATGGGAGGTGATGCATCACATGGACTTCTCGGCCCCACAGAAGCACACCCCCAACTGCCTACCAAGGCAATGAGAAGACCCAGAAGAAAGAAAGGCAGAGGCCTCAAGTTGGATCTGTTCTAAGGCATGGTAGGTTTTAGCACTCTGGTCTCAGACAAGAACCTACTAAGATTTTTTGCAGAGGAGACTGACAGCTGGAACATGCGGTTCTTTCATTCCTCACCATAAAGAGTTCTGGAGGAGGCTGAATTTAGTACATTCATGTACCAGTGGCCGGAGAGGGCTTTGGTGGTATGGTTTTAACCAAGGGGGGTGGGTAGCTGGCACAGCAACCCACAACTTTTTAGCATGTGATCTGACCACAGAGACCAAATTAGAGATGCCGTTTCCAAAAGGAATTACTTCAGTCAATGAATAGGAAGAAGTTGAGCATCCCAGTGTAGAACCAGATGAGCAGGACCTCTTCCCCCTTCACAGATAAGCAGAACCTCTTCCCCCTTCACAGGAGAGCCTAAGTAGACCTTGTGGACACGAGTTCTATGTAGTTGGTCATTGGCACTCGGCTTCAGGATGAGAATATCTGTGGTTGGAGTGTAAGTCAATGGTAGAGGGCTTGTATACTATGTGCGAGCTCCTGGTTCAATCCTTAACAGCACGAAAGCAGGCATCCCCTCTTCGCCCAAAGACGCTCCTCTTACAGAACCCAAGATAACCCATGTATGAGATGGCGTATCCAGCGTGACAATCAAGAAGCCCAAGTCAGTAGCTTCTGACATGAGATCTGTAACCATGACCCGCCTGGGCCAAAGGCCTCCTCAGCTCTCCGGATTTTTCCACAGAGAAGAAGGAATGTATTATTCCACCCACATTGACTCCTCTTGGGTATCATTCACACAGGGTTCTATCGACTACATGCCCAACACAGCCTATCATTGAACAGCTAACCGTCTCTCTCCATAGCTGGCCCATAGCTCCTTCCCCACTGCTACAAATGTATACAAATAGATAAGTGGATTCCACATAAGCAGGCTGGCATTTTGTTAGAAAAGCTGATTTAGCAAAGCATGCGCCACCCCGCCAACCACCAAAGATGAGTGGGCCCCAAGCTTCTGACTGGGCTCTGTATAAGAGAACAGCTCACCAGGTGTGGTGACGTATGCCTGTGATCCTAACACTTGGGAGGTGAAGGTAGTCAGGATAACCCGAAGTCAAAGGCCAGCCTAGGTTACAGGAGACTCCATCGCAAAAGCAAACAACCCAGCATGACCTGGTTCAAGTGTTCAGAAGAGGCAGCTCGATCGGTTAGAGCTGATCCCATTACTCTGCAGGGAACTGCTCATGGAGCCAGGCAGCCATGGAGTAGGCAGAGGAATGGATGACTATCCCACTGTGGAATAAGTCCTCACACACACAAGCCGGAGACTCATGGGGACTCAAGAAAAAAAAAAAAAAAAAAGGGAAGGGCCCTGACTTGGCAAGCAGGGGTCCCTTGGGCTAGCAAGGGCTGGCTTATGTCAGCTGAGAAAGTGAGGGTTGCTGGGAGAGGGCGTGGTCTGACACTATCCACAACCAGCATAAGTCATTGGCCTTTCCTCCCTGTGAGGAGCAGGGAGACAATGCTGGGATTAACCGCAGGGCCCTGCTCCTGCCAGACAAATATTCCACTAGTGAGTGGCTACGTCATATCCCTAAGCCCACTTACCCCACTTCTTCACAGAAGGTCACCAGGGCATCAAAGGCCAGCAGGGTGGCCTTATCAGACGGTTTGTTCCCCCTCTTTTCCTGGAGAGAGAAAAGGACCACTTAGTCCTCCAGAGTCATAGAAAGTGCTCTCACAGTCAGTAATGGAGAAGAAACGTGAAAGGACACCATTGAAGCCAGTTCCCAAGCGGGGATCTGGGCCTGACACTGGGTCAGCATCTCTGAACTACGAGTTGGACTTTCTCAATGGGAGGCGGCAAGAGCTGACATTTCCAAAAGGGTCTCAGGCTATTCTCATGCAAAGGGCCCTGGACTAACATTAGGGAAACTACGAACTTATCCAAGTTCTAAACAGTAAATACATGCACAGACTTTGGGTCCTTACAACGACATATATGTACATATATGCCAATGTGCATACCAGGAGATAGACTAAGCTGTTAACCCCTATCAGATTCAGATTATGCAGTTTTTGGTCTCTTTTATATTTTCCAAAAGTTCCTTTAAAAACTTAGCAATTTAGAGTCAGAGAGGAGCTTACTAGGTAAAAGTACTCGCTACTAAGCCTGAGGTTCTGAGTTCAAACCTCAGGACCCACATGGTGGAGGGAGAGAACTGACTCCACAGTTGTTCTCTGCCCCCCGCCCCCATGTGATCACATGCAATAATAAATGTAAGAAAAAATTCTCAAGAGATGCTGGAGAGATGGTTCAGTGGTTAAGAGCACTGGCTGCTCTTACAGAGGATCCGAGTTCTAGCTCCAGCACCCATGTGGTGACTCACAATCATTTGTAACCCCAGTTCCAGGAGATCCACTCCTCTCCTGACCTCCATGAGCACCAGGGACACATGTGGTGTACACACATACATGCAAGTAAATACTCATGAATACCAAATAAAATCTAAAAATATATATTTTTTTAAATACAGAAAATAAGCCAGGCAGTGGTGACACATGCCTTTAATCCCAGCACTCAGAAGGCAGGGGCAGGTGGATCTCTGAGTCTGCCTGGTCTACAGAGTGAGTTTCAGGACAAACAGACTCACACAGAGAAAACTGATCTCGGGCGGGGGTGGCGGGGAGAAGAAATACATAAAATAGATATCTGAGACAATACTGACTTTTTACTTTTAGGTTATTCATCCCCAACACACAAAAATGCATACTTTGGTCAGGACAGGCAACCATAAAAAACACTAGGAGTATGAAAAAAAGACCATTACTGCACATATATTAGAAGTAATAGTGAAACTCAACCGGATGCTCATCTTCAAAAAAGTATTAGGGGGTCAGAGAGACGGCTCAGCACTCAGTGTTAAGAGCACTGTTGCTCTGGTAGAAGACCCAAAGTCTGGTTCCTATCAGCCACATGATGTCTCACAACCATCTGTTAACTCTAATTCCAAGGGATCTGGTGCCCTCTTTGGGCCAGCAAAAGGCCAAATACACACTCATACAGGCAAAATATTCATGTACATAAAAACAGTAAGTTAAAACATAGTAAAGGGTTTTTTGTTTTTGTCGCCCCCCCCCCCCCCACCAGCAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAGCTCACTCTGCAGACCAGGCCAGCTGGCCTCGAACTCCTAGTGATCCACCTACCTCTGCCTCCCAAGTGCTTTGATTAAAGCGTGTGCCACCACACACACATAGTAAAGTTTTATTTACTTATTTATTTATTTATTTATTTATTTATTTATGTATGTATGTATTTATTTTAACGACTTGTATAAGTGTTTTGTCTGTATGTATGCTGGTGCACCACTTGTGAGCCTGGTGCTCACAGAGGCCAGAAAAGGCCATCAGTTCCCCTCGAGTCTGGAGTTACAGACAGCTGTTAGCTGCCAGGTGGTCCTCTGTAAGAGCAGTCGCTCTCCTAACACCCAGCCATCTCTCCAGCCCCAGGTTTGTTAATGTTATTCTTTTTTTTTTCTTTCTTGGTTTTGGTTTTGAGACAGGGTTTCTCTGTGTAACCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCAGGCCTCGAACTCAGAAATCCACCTGCCTCTGCCTCCTGAGTGCTGGGATTAAAGGCGTGCACCACCACCTCTCAGCTTGCTGATTTTTGTTTTGTTTTTTAAAGAATGTCAGCCAGGGATCAAGTATAGTGACATTGCCTTGCCTAGCACACATGAGGACCCAGCAATATTGATTATATATTTGTGTGCAGTGCTAGGGATTAAACTTGGGCCTTGTCCATGCAGATTAGGCAAGAGTCCTATCACAGAGTTGTCCCTCTGGCCCCAAGCTGCAATGTCCTGTGCGAGCAGCCTTTTCCTCGGTATGGCTATGTCAACATCATTCCGAGTTGAACACATGGTTACAGTCTTACATTTGCCACCATCTCATTTCCTTCTTGGTACAGCATTGTTTCCTCTGAACAACAGTTGCTTCTCCTGCCCTGACCTCTTTAGATCTCGGTGTGACATAATCAATAATTAATTTCAATAAGACCTCAAGATGTCAGGTCAATTTGATCTTTGAAAACATTCTTGAGCCTCTAGCCCCTAGGCCCCAACCAAGCAGCATCAGAGATTTTGCTCCAGGCCAGTGTCCATTCACCTTTCTGGTTTTTCTTCCCATCTACATAGCAAACCCGTTACCCTTACAGCCATCCCCCCAGCAGGCTGTTGTGTCTAGTTTTGTGTATGAGTTTTGCCTGTGTGTATATCTGTGTACCGGGTGCATCCCTGGTACCTGTAGAAGCCAGGAAAGAGTATCAGATCCCCTGGAAGTGGAGTTATGGATGGTTGTGAGCTGCCAGGTGGGTGCTGGGTACTAAACCTGGGTCTTCTGCAAGAGCAGCAAGTGCACTGAACTACTGAGCCATCTCTGCAGCCCTGGGTTTGTGTGTGAGTATGTGGTTTTGTGAGTGTTTTTTGTGTATATGAGTGATTGTGTATGTGTGTGTGTGTGTGGTGAGTCTGTTTTGTATGTGTGATTGTGTGTTTCTGTGATTGTGTGTTCGTGTACTTTTTTTTATAGATTTATTTATTTTTATACATAAGTACACTGTTGCTGTCTTCAGAGGCACCAGCAGAAGGCGTCAGATCTCATCATGGGTGGCTGTGAACCACCATGTGGTTGCTGGGATTTGAACTCAGGACCTTTGGAAGAGCAGTCAGTGCTCTTACTCGCCCTGTCTGTGTACTTTTTAAAGATGCACTTGCTTATGAGTGTTGTTTTTTTGTTTGTAATGTACAAGGATGTTTTGCCAGCATTTATTTCTGTGTACTACGTCCATGCAGTGCCCAAGGAGACCAGAAGAGGGCACTGAATCCCTTGGGACAGAAGTCATTTTAGCCATCTCTGATGCTCCTCACGCTAGACTTTGTGTTGTTGTTGCTGCTGTTGGTTCTGTTTTTTTAGCATGTTCTTTTTTAAATCTTTCTCTCTTTTTTTAGATTTATTTATTTTATGTGAGTACACCATAGCTGTCTTCAGAATTTTTTCTATTTTTAAAGATTTATTTATTTTATGTATATGAGTACACTGTTGCTGTCTTCAGACACACCAGAAGAGGGCTGGTGTAATCGATCCCATTACAGATGGTTGTGAGCCACCATGTGGTTGTTGGGCGTTAAACTCAGGACATCTGGAAGAGCCTGTGTTCTTAACCACTGAGCCAACTCTCCAGCCTTCCCCCACCCCCTACCCAACCCTGCCCCACTCTTTGTTGTTTAATGAAGTCTTGCAAATTGGGGTTTTTTGTTGGTTTGTTTTTTGTTTTGTTTTGTTTTGGTTTTTTCCCCGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGACTGGCCTCGAATTCAGAGATCCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCATGCGCCACCACTGCCCAGCAAATTGGGGTTTTCTTTTTCTTTTTTAAAATATTTTTATTATTATATATTTTCATCAATTACATTTAGAATGCTATCCCAAAAGTCCCCCATACCCTCCCCACCAAATTGGGGTTTTCTAAGCAGACATTTGCTTTTTCTTTTTCTCTTGGTTTATTTTGTTGTTTTTAAGATATAGTATGATTCAACCCCTAGGCTGGCCTTGAATTCACTATGTATCACAGGCTGGCCTAAAACTTACAGCAATCCTCCTGTGTCAGCCCTTTACTCTCACTTGGGTAGGAATCAGAAATAAGTTGCAACTAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACAGTATGCCTAAGGAGGCCAAAAGAGGTTGGATCCTCTGGAGGTGGAGTTAGTTACACATGGTTGTGAGCTGCCTTATATGGGTACTGAGATACAAACTCAACTCTTCTACAAGAGCAGCAAGCACTCTCAGCCACCAGACCAACTCTCCAGCCCCTTTTTTGCTTTGCTTTTTAAAATGAGCATATATTTATTATAACCAGTGACAAGTCCGTGACATTTTCATACACATCTATCATGCACTAGGCTCACAGTCAACAAGAGTGCAGCAGAGGTCAATGCTGGTGTGTTCCTCGATCACTCTACAGTTTTGGCTACGCTGGCTAACCAGGGAGCCCCAGAACCTCCCGGCTCAGCCTCCCTGCATACAATGTCATCTTACCACTCTCCTCCTCCTCTTCAAAGCTGCTATTGTGATTTATAGTTTATTCCTCCTCGAGATAAGTACACATAAGACCAGCCTTAGTTTCTGGGTTTTGTTTTGTTTTCTTTGCTTGTTTGCTGTGGTGCTGGGAACTGAACTTGGCCTTACAAGTTCAACTGGGCAGTATGAATTCTACCACTGAACCATGCCTAGTCCCTTTAGTTTTCTGAGAGGAGGTCTTGCTCTGAGGCCTAGGCTGACCAGGAACTTCCTACTTGGTTGGTCCTGACCCTTCAATCTCTTGCCTCTGCTCCTGAGCTCAGGACTATAGGTCACCAGGACTATAGGTCACCAGGACTATAGGTGTTCACCAACAAGCCCAGCTGGTTTTTACTTAAAAGTCTTGAAATATGTGCTGACAAGATGGCTTAGCAGGTAAATGTGACTTGATTGCCTTCAAAGCTGACAACCTGAGATCAATGCATAGGACCTACTTGGTGGAACAAGAACTGACTCCTGCAGCTGTCCTGATGGCCACACACACTCAGTGGCAGTCCCACCACACCAAACAATGCGCACTAAAGAAGCCACCAGGCATGTTGGCACACCCCTTTAATTCCAGCACTTTGGAGGCAACAGCAGATGGATTCTCTGAGTCAGCCTGCTCTAAAAACTGAGTTCCAGGGCAGCCAGGGGTACACAAAGAAACCTTGTCTTGAAAAACCAAATAAATAAATAAGCAAACAAATCAAGATGATCTTTCTCTCCCAAGAACATAAACTATTTTACCCTTAATTTTAGTTTTTTTTTTTTTGAGGTGCCAATCCTTCTGAAATTCATACCCATATCATATAGGAACTTAATTTTACCTTTTTCTCAGCATTGACTATTCAAGTCTCTCCTCTTCCTGGTGACTTATAATGTACCTACCTCACACCATATTCCTTTCTAGTTTGGGTCTGCTTTGGGCTTTCTATGAATAATTATATAACCAGCAAAAAAGATTTAAATGGCTAAAGAAAAACTTGAGAACATGGAAAAACACTTATGTAACAAAGTTCACTAAATCAAGGCAGGAATAATTTTGGCTACCTTAAAAAGCACACATACAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCTGGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTAACGAGATCTGACGCCCTCTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACATATAATGAATAAATAAATCTTTAAAAAAAAAATTTAAAAAGCACACATACACAATAGTACTCAGCTAAGCTGAATGCTGACAAACGTCTAACCCAGCTTGCCACCAGCAGCCTACTCCCCCTCCACACCATCCTCGTGGGTATGCTTTCTAGGCAGGGATTCTCCTCTAGTTACACAAGCTCTCCAGGATCAGCACGAGCTATATGAATGGACTGGTGAGTTCACACACCAAAGGCTTCTACTGCCTGGGACTTACCTGGAGTTTGGCTAGTTCCTTCCTGATAAGGAAGCTGACTTGGTCTCGGTCATTCCTAGAGTAATACAGGCGGCACCAAGATGGTTTGCCATCCCTGCCAGCACGGCCAGACTCCTGGTAGTAGCCAGCCATGGACTTGGCAATATTCCAATGAGCAACAAACCTGTAGGATGCAAAAGGACTTGATAATGATGGGAGAGCTAAGTGTTCTGGGAGCAGTGTGCCTGTCGAGAGAGTTCACTCCATAGTGGCCAGGTACAGAGAAACTAACACACAAGAGACAGATTCTGGAGTAAGTCACAGGCAGCTGGGTGAGACACCTGTGCCCCTCACAGGCCTCTTGCCATAGAACAGCCCAACTTAGAGGCCATACATAGCACAAGGTATGGGAGAAATTCTAGTAAATATGGCAAATTAAAAGGCTCACAGCCAGGACTGAAGGGATCGCTCAATGCTTAAGAGCACTGGCTTCTCTTCCAGAGTACAGGGTTTGAGTCCCAGCACCCTCACGGCAGCTCACAGCCTTCTCTAACTCTAGATACAGGGAATCCAATCTCTTTTGGTCTCTGCAGGTTCTAGAAAACCAACAAAACCCTTAAAACTAGAGGTCAGGACAAATACTGGATTTAAGAACAGGCTGGGTGTGTTGGTGCACACCTTTAACTAGAACAGTCAGGAGATAGATCTGAAAGCTTGAAGCCAGCCTCACCTACATGAGGTCGTCCAGGCTAGCTGGGGATACACAGACCCTGTCTCAATCAATAAAGATGAGAGGCCAGAGAGACAGCTCAGTTGTTAAGAACTGTACCTCTCTTATCGAGACCCCACGGCTTAATTGGTATCCACGCTGAGTGGCTCACGAGCACCTATAACTCCAGATCCAGATTTGCCACTTTTAGCCTGTATGGGAAGTGCACTCACACGCACATGTATGGGAAGTGCACGCACACGCACATGTATGGGAAGTGCACTCACACGCACATGAACACACCACAAGATAAAAATAAAGCCTAAACAAAAGGTAAAAATTGTTACTTCCAAGTTCTATGATTTTTTTGGGGGGGGGGGTAGTTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTGGCCTCTAACTCAGAAATCCACCTGTCTCTGCCTCCCAAGTGCTGGGATTAAAGACATGCGCCACCATGCCTGGCTGACTTGTATGGTCTTATTTTAGCCTTGGAAGAAGGAACGGGTGTTTATTAAGTACCTATTATGCGTCAGTAACTACATCTGTCTCACTTGTCAGTCAGCATCACATGACTCTACAGATGATAAAGGAGATGGGAAAGGTAAGTGTATAAGAACACCCAGGAAGGAAGAACAGAGTAGGATCTGAGCCTACGGTGGCGACTCCAAAGTTTGCTCCTAACAAGCCCTGGCAGACTGGGGCTCAACTCTTGATTCCATAAGCTGTGATTTCTTAAATGTACAAGTTCTGTTAGTACCGTGACTAATTCCCATCAGTGACATGGTGAGGCTTAATGGCACGTAAAGGCATTATTGATGGCGCTAGGTCAGAGGGCTTCAGGCAGTTGTCTAAAAGCTTTCCCAAAGTCAGATCATGGCTGGAAAGAAAAACCCTCTTCCAGGGAGAGTAGCCAGAGGGAGACTCAGAGGCTCAGCTGAGTCCCAGCTCTTAGGGACTGGCAGTAAACAGTAACAAGGGATGTGAGTAACAAGAGTAATGGGACCTTCCCCTTTGACTCTCCATAGGAGGAAGGGGCGAACAGTTACTGCAGCCCCCTGACAGTTCTACCTGAAGGATGGCGTCAATCAGACTGTTTTTTTTTTTTTTTTTTGGTTTTTCGAGACAGGGTTTCTCTGGTAGCCCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCAAACTCAGAAATCCACCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGTGTGCCCCACCACCGCCCCGGCTTCAACCAGACTGTTAATGGACAACCTTGATAGCATGCTGCCAGGTTCCCTGGGGTAGGGAACCACGACACTCAGAAACTGCAAGCATTCAGCCTGAATACTATGGATTTCCCCAGGAGGCTTCCCTTCTCAGTAGCACTAGGCATGCACACAGACTTGGGACTTTGTACACAGGACTAGGCAATCCACCCGAAGCCAAGCCCTAACTGGGAAAGAAAGGCAAGAACCAGAGCTTACCGGACATTAGCTTTGTCTACTCCCATCCCAAAACTGATGGTCGCAACAATTACAGGGACCTTCTCCTCCATCCACTCGTTCTGTACCTGTGTTCTATCAGATGCCTTCAGGCCTGGAAAGGAGGGGAAGGCAGTCAAAGAAAACTCACATCCCCGTGGGTTTAGTGGGTTTAGTGGGGCAGGGGGAAGTTATTCTGGGTGTGACAGTGTCCTGGACACTTAACCTATAACCTGGATCTATCACTTCTTATCTACATGACCTTGGTACAGGCACTTAACTAAGGAAGAACTTAGCCAGTTAGGCATGGTGGTACATGTTTCATCTCAGTACTTGGGAAGCAGAGACAAGAGGGTCTCCGTGTTTAAAGCCAGCCTGGTCTACATAGTGAGTACTAGGACAGCCAGAGATACATAATTAGACTTCATTTTAAAAAAATCTTAGCCCACAACTGGAAAAGAATATTTAATAAAAATGAGACCTAAGAACTTATGTCCATATGTCTCAAAAGTCTGTGGTCTAGATTTAATTTTTTTTAATAATATTTGGGCCCACAAAAAAAAATCACCTCTGGCTAGAGAGAGGGTTGACTAGTTAAGAGTATGGCTGCTCTTCCAGAAGACCAGGGTTTGATTCACAGTGCCTGCCACACCACCTCCAGTAGGGTCTGTGTGACAAGTTCCACAACCTCAGGCGATTGACCTGGGGTGGGGGCGCTCCTGGAGCCAGTTGTGGAACTCTTCACCAAAGAAAAAATATTGAGCTGTAGTTCTCAGGGCAATCTATCTGTTTTGCTTTCTCTTATTCCTTTTCTTATTTACATGTTCCGAGTAGCCTAGGTCAAGATCTTAAGTGAGAATAATGGGTAAGAAACCATCCCTAGAGATGGGGGTCACTAACATGACCTTATCCCCGAGGAAACCAACGTTTTGCTTTCTAAATTATTTACAAAACTATGATGATAGATAAAAACTTTCTCAACAATAAAATTTCATATGGCTACACGTGTGTCATCGGAGGTAGGTTTTGGTATATGGTAAAGAAAGCCTTAATTAGAAAGAATTAAAATAGTTTAAATTTATATATTAAAGATTTATTTAAAAATCAGGCACTAGATATTAAATAATAATTGACCATGGGGCTGGAGAGTTGGTTCAGCGGTTAAGAGCACTGACCACCATTAAAGATTTATTAACCTGCTCCTGGAAACACGCCACTAGAGTTGGATGACAGCCCCCAATGAACACATCCTTTTAGAATTGTTCTATTTTGATGATTTATAATGAGGGTACCTGTTCTGTTGTGATCCACTGAACACATAGTTTCAGAATTGCAATGCTTAGATCATTTTTTGTGCTTTACTGTGCCAAATGATTTTTGTTGGTATTTGTGATTGTTTCACTGGATACAATTTCTAAGAGATGTAATGTTTGGTTTTTTTAATGTATAAAATCCCCTGCTTGAGAGCTTCAAAATACACTCATACTCAAACTGTCTCTGTGTTTGTTTCTGTTTGTCACCGCCAAATCCTTACCCACCTAGCTTCGAGGACCCTCATTCCAGGGACCCCCATACCCAACTGGGGTGGTCTGTGGCAAGCACCCACATTACAATTCTCAACTATCTGTACTCCAGGGGATTCAATGCCCTCCTCTGGTCTCTGTGAGTACTAAGCATGCAAGTGGTATACAGACAAGCATGAAGGCAGAACACTCAAACACATAAAAAACAAAAGCTCTTTGCTTCTGCTGGGTAGAGAAAGAAGTCAGAGTCACCGGTTTCTAGTTTTTAGTGGGATTGAGTAAATCACTTTAGCTCTATATGAGTACCTAGAAATAGGTGACAGATCACCTGATATTCAGGATCCATTAAGGAGCACGCGAAGAGGCATGCGGCGATGAAGAGGATAGAGGTGAACCTCTTACCTGCGTGGTAAGCCTTGGCGTTCACACCCCTGCTGCTGAGCTCAATGGCCAGTTGTTCACAAGCTTCTCTAGTCCTGCAGTAGACAATGCCACAGCCAGAGGACGACTGAGCAGGAAGAGGTGGGAGGGTGTCAATCAGGGGAAGGGTGGTACAATCAGCCCACTCACTAGAGCACAGCACTCTTCAAAAGCTCCATGCTCTCTCACCAATGTCTCCGTCTTCTCTGAGGCGGGACAGCCAACAGGGCTCATCACTTAAAAAAAAAAAAAAAAAAAAGCAAGACCAATCCTGGCATAGTCTGGTGTATGCCTTTAATCCCAGCAATAGGGACAGGGCAGATCTCTGCTTTCAAAGCCAAACTGATCTAAACTAGGGGGTTCCTTAACACCCAAGGCTACATAGTGAGACCTAGCATCCAAAAAAAGGTGAGGGAAGACCAAATCGAAAATCCAAAATCCAGCCTTTCTAATTCCCATCTCCTCTCTGAAAAGCCTATGTAGGCCTACTAAACATGCGAGGACTGTTTACCTGCCTATCCTAGGATCTGGGACCAGAAACTCCCAGCTAAGACAACTAGGAAACAGTAGCTGTGTCTGTGTTGCAGACAGCATTTTGGACTGCGCACCCCAGCACCTCACCCCGTTCTCAGCCTTCTGTCCAAGAGCCTTAAGGCAGAAGTCCCTCAGATTGCCATAGACGTCGGGAATCAGTTCCTTGAACTGCACATCATAGAAGAGGTTGGCCCGGAAACAGGGAGTCTTGAAAGAGGCGACTGGCTGCTTCAAGTGCAGGGCAGCAAACACATCCTCTTGAACCTGTGGGGTGGCTGTAGCCGTCAGAGCCACGCACGGGGCATGTGCCAAGCGGGAACGTAGGGCACCCAGACGCAAATAGTCAGGACGGAAGTCATGTCCCCACTGGGAAACGCAATGAGCTTCATCCACCACCAAGTAGGAGAGCAGGTTTCGGGACACCAGGGAATTCAGGGTGGGCTGGAAGGAGGCTGAAGCTGCCATCTCTGGGGTGATGTACAAAAGTTTGGTCCGAGGTTTGTCTCGCTCCAGGTCAGACAGCAGCTCCTTCCTCTCCTGTACCGAGAGCTTTGAGTTCAGGGAACTAACTTGTACCTTCAAGGCCAGCAAATGGTCTACCTGGTCCTGTGGGTAAAGAAGAATACGGAGTGGAGCATCATGAGTCCTCAGGACCAGGCAATCGGCACAGCATCTCAACCACGGACCATGAAGGACCTCCAGCTACCCTCCAATATGTCTTCTCCTTGCCCAGCAGAAAGGATGGTAAAATGGATGGGAGGCCAGGCTCTGGGGTGAGAGGAGCTGGATCTATGGTGGCTCACTTAGCTCCGTTCCTTCTCATCCACCACACGCACCCTGCTGCCCACCACAGTAACTACCTGACAGGGCTGTGATGAGAATGAAGTGAGACTTTGCTCCTTAAAGGTGAGATGGGAGATGGCGGTATAGGCTTGTAAGCCTAGCACTTCAGATACAGAAACAAGAGGATCAGGAGTCTGAGGCCAGCCCCACCTATAGATGTTAAGGTTGAGGCCAGGCTGGGCTATATGGGACCCTGCCTCAAAAGATAAAAAAAAGAACAGATTGAGGATGGGCGTGGGAGGTAGCACACACCTTTAAACTCAGGAGGCAGAGGCAAGGTGAATCTCTTGAGTTTGAGGCTAGCCTGGGCTACATAACAACTTCCAGGATAGCCAAAGCTACACAGAGAGATCCTGTGTTGGGAAAAAGAAAAGGAAACAAAGAAGAGTGAGTGGAACGGGCGCTCCCACTCTTGGTACAAAGCCTTGGTGTAGTGAGTATCACCGAGTGCTGCTGCTGCTCTCCACCTCTCTGCCTATGTGGTCGGTGG
GF3 -1 -1 1 GAGTCACGAGGTAGTGGAGCGTGACAAC
GF4 -1 -1 1 GCCAGCTGGAAGGCTGGCGAGTCTCCTTC
EX52 -1 -1 1 CTCAGATGAGAGAGAGCAGCAGTG
EX32 -1 -1 1 CTCTCTCTCATCTGAGGCAGAGCAGAGTTC
GR3 -1 -1 1 CTTACAGAGGACCACCTGGCAGCTAAC
GR4 -1 -1 1 GTAACCATGTGTTCAACTCGGAATGATG
Comments:
Created
Target: 2803Ensembl ENSMUSG00000075420 -- 115774697 -- 115775231 ENSMUSE00000646170 -- ENSMUSE00000646170
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: chromosome:NCBIM37:11:1:121843856:1, start 115774696: end 115775231
Found 10 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
Entered all non-repetitive oligos
Sequence count unchanged by blasting
finished aligning oligos to bac
ACCEPT (0) gap pair G5:11:115768197:115769196_467 G3:11:115778731:115779730_18
ACCEPT (0) gap pair G5:11:115768197:115769196_467 G3:11:115778731:115779730_657
REJECT (1) gap pair G5:11:115768197:115769196_467 G3:11:115778731:115779730_8
REJECT (4) gap pair G5:11:115768197:115769196_857 G3:11:115778731:115779730_18
ACCEPT (0) gap pair G5:11:115768197:115769196_857 G3:11:115778731:115779730_657
REJECT (4) gap pair G5:11:115768197:115769196_857 G3:11:115778731:115779730_8
ACCEPT (0) gap pair G5:11:115768197:115769196_684 G3:11:115778731:115779730_18
ACCEPT (0) gap pair G5:11:115768197:115769196_684 G3:11:115778731:115779730_657
ACCEPT (0) gap pair G5:11:115768197:115769196_684 G3:11:115778731:115779730_8
DESIGN COMPLETE
U-type: U1
D-type: D1
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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