High Throughput Gene Targeting: Create Design

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Design Type Oligo Select Method Artificial Intron Design
5' retrieval block size U block size Min 5' spacer Critical Exon Min 3' spacer D block size 3' retrieval block size
 
       
Subtype Domain Disrupted
Gene Build Gene
Start Exon End Exon
Target Start Target End
Primer Length Design Comment
Created by erb
Valid After Annotation
not done
oligos on fwd strand oligos as ordered View gene and design in Ensembl UCSC BLAT recombineering primers
Design ID: 109205
Chr: 5
Strand: -1
Status: Ordered
BACs: RP24-364K12 : RP24-364O12 : RP24-208L2 : RP24-91G18 : RP24-240N13 : RP24-547J21 : RP24-126C23 : RP24-252P5 : RP24-302O23 : RP24-138H19 : RP24-113D4 :
Instance: Plate 115: Well H01: BACs RP24-208L2 RP24-91G18 RP24-364K12 RP24-364O12
Oligos on GRCm38:
Type Start End Length Seq
G5 120912732 120912781 50 GTCAGACTTACCATTGAGGTTGGTTTTGTAGCGATGAGTGATCCTTGTTC
U5 120906863 120906912 50 ATCCAGTCATGCAGCATATGATGTCCTGGTCTGTCCTCTGTGTCACATTT
U3 120906799 120906848 50 TAGGTACCATTGTCATGAAATGCGATGCGACAGACCCAGAACCAAGTCCA
D5 120905284 120905333 50 TAAATATTCACTAATATTTTAGGGCATGAAAGGATATGAATATAAAAGTT
D3 120905130 120905179 50 GTATCATATAAGGGCTTTTGAATATATTTCTTAATGACCCATTTTAAATA
G3 120902008 120902057 50 GATGGCATAGATTCTGGGATCAGGCTTGCTGGAAGTATTAACATGACCTA
Oligos:
Type Start End Length Seq
D5_D3 121355189 121355292 104 ATTTATGCTCTTTTACTATGCTTAACTCCCAAGGAATATTTTGCATGTTTTGAATCAACTTAGTATCCAACATTAGATATAGGATCCTCAGTTACAGATAGTAT
G5 121362741 121362790 50 GTCAGACTTACCATTGAGGTTGGTTTTGTAGCGATGAGTGATCCTTGTTC
U5 121356872 121356921 50 ATCCAGTCATGCAGCATATGATGTCCTGGTCTGTCCTCTGTGTCACATTT
U3 121356808 121356857 50 TAGGTACCATTGTCATGAAATGCGATGCGACAGACCCAGAACCAAGTCCA
D5 121355293 121355342 50 TAAATATTCACTAATATTTTAGGGCATGAAAGGATATGAATATAAAAGTT
D3 121355139 121355188 50 GTATCATATAAGGGCTTTTGAATATATTTCTTAATGACCCATTTTAAATA
G3 121352017 121352066 50 GATGGCATAGATTCTGGGATCAGGCTTGCTGGAAGTATTAACATGACCTA
G5_U5 121356872 121362790 5919 ATCCAGTCATGCAGCATATGATGTCCTGGTCTGTCCTCTGTGTCACATTTGACTTAGTCCATGTGACCCTGCCATCCTGGGGCGTGGACTGGGCACACTGGACATGGAGAAGGTAGTGTAGTTACCCTAAGTCACACAGCAGTTCAAAGCTTGGATCTGCCCCACCCCCATGGCTCCCAACCCTGACCAGCCACTCTCCAAGAATGCTCTGCCATCCTGGGCTTCAGTTTCCCTGACTCTGAAATGGTCTTAGCCTGGGACTGGGGAACACAAACTGTAGCTGAGGCTCCTTCTCCAAAACAGACGTGGGTCCTTGGGGGAAGAACCCATCGCCAGCTGTGATCAGAATTGAAGAGGAGGGATCAGGGCTGTCTGGCTTCTGAGAGTCCCCTCCCACCCCATTCAGTCAGCTCAGGCAGAGGAGGGCTCACCTTCACCACCTTGGAGACCCTCACTGGGTGGGCAGCACCTTGGAAGCATCTCTCCTTCAGGAAATCACACACGACATTGACGGCTGATTTGACATCAGCACCAAAGGTGGTGTCGGGAAGGAGGTAATCCTCTATGAACTTGTCCAGCGTCCAGGCTGGGATGCTCCTGAGTCCGTGCTCCATGCTAAGTCTGGAGCTTCCTGGGTCTCCCAGCCTAGCTGAAATGGGAAGTAGATTTTCAGTTTCCATTTCCCGAGAAGGGCAGGGCTATGTAAATAGGCCAGGCCCCGGCAGCCAATGGCTTCAGAGCTTCTGACCAGGTTCTTTGAAGTCTGAGCTTTCTTAGGATCCCACCTAGGAAAGAACTTTTCATAAGGAAACTGATCTGGCTTTCTTGGGGTTTCTTCTGGGGAAAAAAAAAAAAAAAAGGTGTTACATGGCATGTTCTACGGGCAATGTGTACACATAGTAGAAGAGGGGAGACCGAGTCAGGAAACTGCAGAAGGTGGCTGTGGCTTCCAGCTCCTGAAAACGTTTTAGGCGGAACTGCATGTTTTGTTTTGTTTTTCTGTTTTTCTGTTTTTCTGTTTTTCTTTCTTTCTTCCTTTCTTTCTTTCTTTCTTTCTTTTTCTTTTTTTTTTTTTTTTTTTAGAACAGCACGGTTAAGGTTTTGTTTTTCCTTTACCTCTGGGCCTGCAGTTCTTTTTTTTTTTTTAATTTTAATTTTTTTATTAGGTATTTTCCTCATTTACATTTCCAATGCTATCCAAAAAGTCCCCAATACACTCCCCCCACCCCCCTGCAGTTCTAGGATTACAGACAATTCTAACAGAAAGCTCAGACTTCCCAGAAGCCCCCTCTCTTACTCACATCTTACATTTTCCCCTGTGGTGCTGGGGGTGGGGGGCGGTCGGGACGAGAGGGGGGTTTAACCCAGGACATTCCCAAGGTTGAAGTACAGGCTGTACCACCAATTGGGTCAGGACAAGTCAGCAGTGAATATTACAGTGAGCTGTCTTTCAGGGCCACTGCAAGTGTGAAAGTCAGCCTTTGGGTGACACAGGTCCTGGAGGATCACTCATGGGCCACCACCCAAGGCTGCATCAGAATAGTTTGATGACCTCCAACCCCTGCCTCTGTCTCCTCGTCACTTGTTTTGAGTTTTTCACTTCGTAGTTGTTGTGGTCCTGCTGTGAGGCCAAGGCTGGATTTGAACTCATGATTCCTCCAGGTTGGTCCCCCTCAGTGCTGGTGTTACAGGGTGGCCACTGTGCCTAGCTGTGCAGTGGATTGCCTTGGTGCTGTTTGTATTTTGATGCTAATCCTGCCCCAGATGAGGAGCAGATCAAATACTGAAGAGATTTCCATATGAACCTGCTCACCACTTTAAGTGGGCAAAGAAAGGCTAGAGCCTTCGATTGGGCAGTGGAAAAAAAAAAGGCAGGGCAGGAAGTTTTAGATGTGAGAGGGGGGGAGGGGGAGAGGGAGGAAGGAGACTAAGGAGGAGGGGAAGACAAGACAGAGGGACAGAGAGAGGGGAAGATGGAGGAAGATGGAGCAGAACCACGTGACCTGGAGGAGCTGCAAGTAGCAAGGAATCTTATAGCTGGGGAGTAGTGTAATGTTATATGTGCCCAATCTAGGATGTGCACCTTATAAATATTATAACTGAGTTGTGTGTTCTTGGCATGGGCATTTTGGTGTTGAAGATTTACTGATACAAAACTGACTGGTATTCAATATCAAGGCTGTCTGGTGTTTCCCATTTACGACGATTTAGGTGGGCAAGAGAGAGGGCAGAACAGCACGGAGTGGCCACTGCAGGTCTGCAGACCAGCCACAGGGGTAGAGAGCCTGTGGAACTGGAGAGATACCTCCAGGCTTTCTCTGGAAGGACTGGTTTGGGTGGCTTAGCAAGCCAGCCTTGGAAACCAGGGTCAGACAGCCAATTTTTTGGTATCCAGCAAGGAGAAAGGAGCTACTACTGGTCCCCGAGCCTAGTCTGTCCAAGCTTGGGATTTATCAAATTACAGTGTCGTGTGCAATGTGTGTTAACTCATGCCTAAGGCAAAATGTCACCACGAGGGCTCAGGCAGATGAGATAGATGGCGCTATGCTAAACTGTCACCATTCAGCAAGACTATCAGTTTACTGTGTGTGTGCTTCTGTAAATATACAGATAAAGACCCATGAGGGGGTGAAGAATTTGTTCCTTAGCAAATCAACACTGTGGGGCTGGAGACTCAACACTAAGAGCACTGGAGACACTGAGGTCTACTGAGTGCAGGAAAATGAAATTGCGAGCAATCTGGAACCAATGATGACTTTAAGACTTAGTTGTCCATGTGAGGATGAGATTTGAAGCACTGAATCTAATCACAGTGCACTGACTCAAACCCCAAACTAATTTATTCTCTCTCCTTCCTTTCTTAATTTTCTTCTTTCCATATTTCATTCTCTTTTTTTTCCTCTCTATTAACTGGATCTTATTATACATCCAGGTTGGAATTAACAGAACTCCAGGTATCTCGGGGTCCCAACTTCTGGAATTACACACACACACACACACACACACACACACACACACACAGTCACCACAAATCACACAGAGACCTAATGGTTCCACAGGTCAGTGTGCTGAACCAGCATCTCGTGAATACACAGAGACATTAGCCACAGAAGACCCCACATGTGCTTTGTCCACTTGGGAGTGTGAGCTGCAAACTTAGAAGGCAGGAAACTGCTCAGAGCTACCAGCTCCTAGCCCCCTGCTCCTGTTCCCACACCTTATTCCTGTGCCCAATGTCCACTGTGGGAAGCCATATGTGCCGTTGCAGAGTGGCGCTGGCTACTGCTGGCCACAACGCATAAGTTTGGGCAAACAACCAATGTGTACATATGCCGTAAAGTTTTTTGCCAAGACACTGTCTGGCTCGGGCATGATAATGAGGTTCTGAGAGTATAACCAATCAGATGTGAGACATGCAAATGAAGTATGATAATGAGATTCTGTGAAGTACTGAGAGAGAGTAACCAATCAGATGAGGAACATGCAAATGAGGTATAGTGCATAACCAATTCGGGTGTGAGACACGCCTCTCCTAGACCTATATAAGCAGCACCAGTTCTGGGCTCGGGGTCTCTTCCCCTCTGCAATCAAGCTCTCCCAATAAACATGTGCAGAAGGATCCTGTTGCAGCGTCGTTCTTGCTGGTCGAGTCCGGCGCGTGTAAGAGTCCACTCTCAATCTCTGTGAAGGGGGAGTCGGTACATTTCCCTGGCCTTGATTCCTAGAGTTCTCTCTCTAGCAGCCATGAGGACCAGGAGCCCAAATGCCCATGGGTGTTAGAAGATGCATATCCCATGGACAGAGAGCTCTTCACACTCTGCCAGGTGCTCTATCTGAGCCTTCAGGAAACATGGGAATGTGAGTCTATTATTCCAGCCTTGACCCTCTGAAACATCATCAGATCCATGGTTTTAATAAACCATGGCCACAGGTCTCCTGCCAAGCTGTGGACCCATCATGGCCTCACTGAGACTCCCACTTAAGTCCTTTTCTCTTCTACAGCCCCTTCTGCACTCACACTGGACACTAGCTTCCAATTGGACCAGACCCTGCTCTTCTTGGGCCTGGTTCTCGCCTTCAGGTCTGCCATGCAGTGGCTCCCTCATGGTGAGAAGGGAGACACAGGCCACAGCTCCTCCGCCCCATCCCTGACCTCCTCTCCAGCAGGACACCTTCAGAGGAGCCCTGGAAGCACAGCAATTGTCCCAGACCCTGCCCTCCCCTTCTGGTCTGGCTTGTACCTCATTCTTAAGCACCACAGAGAAACAGGGACAGTCTCTGGCCTTGGCATGGCCATGCCTGTGTTTTGATGACATCTGGACACCACTGACAAGGAGAGGCAGAACCTGGGACACACAGCAAACCTGGGTCCCACATTGCTCATTTGGATTGTCCTGTCCTGAGGCATTTGGCATCCTTTCCTGATGGGCTCAATGCACAGTGTGTGGTGGGAGGGTAGAGGCTGAGACTGATGGCTCCAGCCAGTTGAAGACACAGGTGGAGGTGGAGTCTTGATTGTGACACTGGAAAGGACCCACCTGCTGATGTCTGGACTTTGGGTTTCTAACCCAGAGCCTTTCTGCTCTTTAAACCCCATTGTCACTTTGCCACAGGCAAATCTACACAGTTTGTCCCTGCAGCCTGGGATGCACCAGGACCCTTGACTTCTCAATGACACTGCATCTGTCACTCACACACAGAATCTGCTTCTCTTGTTTCTGTTTCTTCTGGGTTTTGTTCCGACCTCTGGGTCTTTGCAGAGTCTGTTCCCAGCACCCTGCATGCTGTTCCACTTTTGCAATTGGCTGGAGGTCCTGAGTTCTTCTGTCCTTGGCTAAGAGGTCCCTGCCTCAGGGAACAACTCAGGAGCCCTTTCCAGGGTCCCAGGTAGAATCAGCTGCCCTCCCTACCTCCCATAGAGTCTTATAAACTGAACTTCCTGAGAAAGGGGAGGAAGAACAAGGAAAGCCATGTGGGTCCTAAAGACTCTCCAAGGCTGAATATCTAATTAGAACTTAGTGATGGAGAGTTGGAATGTTGACAAGTCAGAGCAAAATTATCTTTCATTCTCTTCAGCACTTTTAACAGGCACTCACTGTCTGCCACTGTATCTTATCTAGCAGGGAACCCTGCCAATAAGGACTGGCCTGGCTAGATCTGACCCTAGGTGCATGAGTGGCAGGGATGCTATAGCGAATAACTGAACACATTCTCACTGGATGTGAGGACCACTCCACAGGAGGACACTTCAACCTGGTACTACACATCTGGTGGGGAAGCCACCACTGGGCGATCACAGGCCCCCGGGATGAACCTACCAAAGCGATTTTGGTCAGCAGACAGAATGTCAGGCTGCTCCTTACATCTTTATACCTGATATTATGTAGGCATCCCTCATCTACAGTTCCTGCTTCTCCATGACTCTAGCTTGAGTTTAGTTGGCATTGAACCCAGCCAGTACAGAGTCAGTTTTCCTTGGGGTGTGGTCCCTGGCAGATTACCACTTCCCAGTGGTCGGCTTCACACCTATGTATGAGAAGCACGGACTGGATCCAGTGGTTTACTCATTAAAAGAATCACACTAATTCGGGATGAGGAAGCGTTTTTTGGGAGGAAAGGCGAGGGTGAATTTGATAAAATATATTGAAGGTATATTATGATAACTTCAAAGAATAGTTTACAAAATATACTGGAGGATATTTAATTCCACTATACTTTGCCTTTCAAGAGGCACAAACCACTTCTGCAGAGCTGGTCAGATCCCCTCAGGGGGCTCCTGAGGATATCATGTCTCCAGCAGCTGCCGGACATGTGGCCAAGAGGGCAAATCCAGGTCTTACACCTTGGGACCTGGACACTCCCTGACTCAGTCAGACTTACCATTGAGGTTGGTTTTGTAGCGATGAGTGATCCTTGTTC
U3_D5 121355293 121356857 1565 TAAATATTCACTAATATTTTAGGGCATGAAAGGATATGAATATAAAAGTTGAACAAATTTTATGTATTATTTGATTCTCAAAATAATCAACAGTATTAATATGTTTGATGTATGAAATGCATTTAAATTAAAAATATTTAAGAAAATAGAGATGAATTACTCTAGAGCTGTGTTAACCATGACTCAGGATCACACCCACATACATGTGAACACCTATGCAACACACATGTGCACACACACAGAGGTGGAACTGGAAGGGAGGATGAAGGCATGCAGAAAGATGAGCTGAGGCTCTCTGGCAGGCAGGCTTACCCAGGACATCAAAGGCTGGCAGCACATCAAACTCCACCTCCTGATGCAGATGGGGGGCGCTCAGCTTGAAGCTCAGAGACCGGGCGTTGGGCCACCATGAACTCTGGACCTCAAACTTGACTCTAAAACGTCTCTCATGCTGAACCTCGTACAGCTGTTTCTTAATTTCCTTGATGAACTCTCCCCGTCGGTTTAACTGATCCTCAAAGCTGGTGAGATTGTTAAGGAACACCACCAGGTCAGCGTCTGACTTGCCCTTGAGTGTGGTGCCTTTGCCTGAGGAGCCACCCTGAAAGACAAGGAGGAGAATGACAAGGGACAGGGAGAGCCGTGCTGGGCAGTCCAGATGCTGAACTAGAGTCCACCACTAAGGGTGATAGATGGATGGATGGATGGATGGGTGGATGGATATATAAAGCTGCCACATGTGTAGTGGAATACTAGTTAGCCCTGAAGAGTGACATCAGGATCTATGTAGGAAATATAGGCCAGCTCAGAGTCTCCTCCTGGTACAGAAGATAAGCCAGATTCAGAAGGACAAACATCCATGTTCATCTGTGGAGTCTATATTTGTGTATGTCAGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGTGGGGGGAGGGAAGTGTGTATGGGAGTGGGGGAAGGGAGAGATCTAAAGGAAATGAGCAAGGAATAGTGTTACACTATAACACAGTGTACTATGGAATAGCATATAGAATCATAGAGCAACTGAAAATAATAAAAGTGATCAACATCTAGAAAATGAATATTTTCAAAAGAAGAATTTATACTGAGAAGAACCTCCCTAAATAAAATGCTGAGAATCATCACTAACACACCTGAGCACCAGCCACTGTTGAGAGAGGGCATTACAAATTGTCACATCTGCAGATGGAGAGGTGGGTCAGTAGTTAAAAGTGGTTGATATTCCTGCGGATGACCTCAGTTTAGTTCCCAGCACCCAAGTAGGCCAGCTCAGAGTCACCTATGTAACTGCATCTCTAAGGGATCTGACGCCCTCTCTTCAATCCTGACCTAACCTGCAGTAATGCATACAGAGCCCCACTCACAACTGAAAATATAAAAATACAATCAACTAAAAACAAAAAGAAAGAAAAGAAAACAACTTTGCTTCTGCACCCTTCACAGAGCCTGTGAGTTAGGTACCATTGTCATGAAATGCGATGCGACAGACCCAGAACCAAGTCCA
D3_G3 121352017 121355188 3172 GATGGCATAGATTCTGGGATCAGGCTTGCTGGAAGTATTAACATGACCTATAAAGGAGATTTCATTCATTTTAATATCTCAGCCATTCTATGAGGTTCCTCAGTCTCCCCAGGTCTAGAATCACTGCCTATGGTGTTCAGTGAACACGCAGAGTTTCTCCAAAGCAGACATCACTCCACATTCACTCCCAACTACCCATCAGCCCCACCACTCCTCAGGAGTGCACACATGCTGTGCACTACAAAGTCCATGCTCCCAGTTTGTTCTAGTGAGAGGGTCTTTCTTGCAGGCCTGGAGACAGTGGTGGGAAGAACCAGCACACTGTGGTCACACACACGGTTGTCCGATAATGGTCAACCATTATCCAGTATCTGGGTTCAGCATCTTCTGCATGGTTCTTGTCAACGCTGTGCACTCATGTATGTGTCAAAGTTTACCGTGGGGCAGTCGGCTGCACTGGTATATCATGTCTCACGCTGACAGAGTATCTGCCCTATTTTGTGTATAGAAACTAAGGTTTCACTGGGTTTTCACTGAACTATTTTCTGTAGTGTGTGACTTAATATGGTGTGGTTTTGTTTGTTTGAGCCCTGAAAAGAACAAGATACGGCAGTGTTCCTCAGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGTGTGTCCTGTGTACTTTGTACAGTGTATGTGCAACAGATATTAGTGTAGGTGTATGCACACGTGTGAGTAGTCTAGGCAGGCTGTGCCTTAAGTGTGTGCACACGTGTGACATGCAGCAGGCCACATCATCACACTGTGATGCTGACACAGGGCAAAGTCACACAAGGATGCAGCTTAGAGAAGCCAGTCCCTCAGTGACCAGGGAGGTCTCTCTTTCACTGGCTTATTTTCAGTGTCAGGGTCTAGAATACAATGAAGCTTATCACCTACAGAATACAAGAGACAAAAGAGAGAATCTCAGGGGCAGAAGATACCAAAGAAAAGATTAACACAAAATAAAAAAAAAATGCAAAAAGCAAAAAGCTCCTAACCCAAAACATCTAGAAAATCCAGGACATAATGAGAAGATGAAACCTAAAGATGATAGGTATAGAAGAGAGTGAAGATTCCCCACTCAAAAGGCCAGTAATTATCTTCAACAAAAATATAGAAGAAAACTTCCCTAACTAAAGAAAGGGATGCCCATGAACGTACAAGAAGCCTACAGAACTCCAAACAGACTGGACCAGAAAAGAAATTCCTTCCATCACATAATAATCAAAAAACCAAAAGCACTAAACAAAGAAAGAATATAAAAAGCAGTAAGGAAAAAAGGTGAAGTAACATATAAAGTCAGACGTATCAGAATTATACCAGACCTCACCAGAGACTATGAAAGCTAGAAGATCCTGGATAGATCACATACAGACCCTAAGAGAACACAAATGCCAGCCCAAACTACTATACCCAGCAAAACTCTCAATTACCATAGATGGAGAAACCAAGGTATTCCATTTCAAAACTAAATTTACACATATCTTTCCATAAATTCAGCCTTACAAAGGATAATAGATGGAAAACACCAACACAAGGATGGAAACTACACCCTAGAAAAAGCAAGAAAGTAATCTTTCAACAAACCCAAAAGAAGATAGCCACAGAAACATAAATCCACCTCTACCAACAAAAATAACAAGAAGTAACAATCACTTTTCCTTAATATCTCTTAACATCAATGGACTCAATTCCCTCACAAAGACATAGACTAACATACTGGATGTGTAAACAGGATCCAGCATTGTGCTGCACACAAGAAACACACCTCAGTGTAAAAGCACTACCTCACAGTAAAGACACAGACTACCTCAAAGTAAAAAGTTGGAAAACAATTTTCCAAGAAATGGTCCCAAGAAACAAGCTGAAGTAGTCATTCTAATATAAAACAAAATCAACTCTCAACCAAAAGTTTTCATAAAAAGATAAGGAAGGACACTTCATACTGGTCAAAGGAAAAATCAACCAAGATGAACTCTCAATTCTGAACATCTATGCTCCAAATGCAAGGATATCCACATTCATAAAAGAAACTTACTAAAGCTCAAAGCACACATCACACCCCACACAATAATAGTGGGAAATTTCATCACCCTACTCTCAGCAATGGACAGATCATGGAAACAGAAATTAAACAGGGACACAGTGAAATTAACAGAAGTTATGGACCACATGGATCTAACAGATATTGATAGAACATTTCATCCTAAAGGAAAAGAATATACCTTCTTCTCAGCACCTCAAGGTACCTTCCCCAAAATTGACCATATAATTGCTCACAAAACAGGCCTCAGCAGATACAAGAAGACTGAAATAATCTGATGAACCCTATCAGATCACCATGGACTAAGGCTGGTCTTAAATACCAACAAAAACAACAGAAAGCACACATACACATGGAAATTGAACAACATTCTACTCAATGATAACTTGGTCAAGGAAGAAATAAAGAAAAAAAAAATTAAAGGCTTTCTAGAATTTAATGAAAATGAAGACACATCATACCAAAACTTATGGGACACAATGAAAGCAGTGGGTAAGAGGAAAACTCATACCTCTAAGTACCTTCAAAAAGAAACTGAAGACCTGAAAGCTCTAGAACAAAAAGAAGCAAATACACTTGATAGGAGTAAATGGCAGGAAATAATCAAACCAAATAGAAACAAAAAGAACTCTACAAAGACTCAACAAAACCAGGAAGTGTTTCTGTGAGAAAATCAACAAGATAGGTAAACCCTTAGCCGTACTAACAGAGACCACATCCAAACTAACAAAATCAGAAATGAAAAGGGATACAGAACAATGAAGTATACCTTAAAATAACTATTCTGTTTGTTGAATATCATGTTCTACAAACATCATGGAAATATTACTCATAATTTTACTCACTGTGCTTGAAGTTAGCATTTTTGTAATGTTTAACTTCAAAGAGTTTTTGCTATTTTAAAATTTATAAAATATATTTACTGATAAAATAATTTCTCTCCTAGAAACACTGGTAATCTTTTTTAAGTAAACTGATTGTTAGACCATGTACACAGATATATAATGCCTTTCAAATACTCTCTTACTATGTCAGTGGTATCATATAAGGGCTTTTGAATATATTTCTTAATGACCCATTTTAAATA
U5_15 121356872 121371922 15051 ATCCAGTCATGCAGCATATGATGTCCTGGTCTGTCCTCTGTGTCACATTTGACTTAGTCCATGTGACCCTGCCATCCTGGGGCGTGGACTGGGCACACTGGACATGGAGAAGGTAGTGTAGTTACCCTAAGTCACACAGCAGTTCAAAGCTTGGATCTGCCCCACCCCCATGGCTCCCAACCCTGACCAGCCACTCTCCAAGAATGCTCTGCCATCCTGGGCTTCAGTTTCCCTGACTCTGAAATGGTCTTAGCCTGGGACTGGGGAACACAAACTGTAGCTGAGGCTCCTTCTCCAAAACAGACGTGGGTCCTTGGGGGAAGAACCCATCGCCAGCTGTGATCAGAATTGAAGAGGAGGGATCAGGGCTGTCTGGCTTCTGAGAGTCCCCTCCCACCCCATTCAGTCAGCTCAGGCAGAGGAGGGCTCACCTTCACCACCTTGGAGACCCTCACTGGGTGGGCAGCACCTTGGAAGCATCTCTCCTTCAGGAAATCACACACGACATTGACGGCTGATTTGACATCAGCACCAAAGGTGGTGTCGGGAAGGAGGTAATCCTCTATGAACTTGTCCAGCGTCCAGGCTGGGATGCTCCTGAGTCCGTGCTCCATGCTAAGTCTGGAGCTTCCTGGGTCTCCCAGCCTAGCTGAAATGGGAAGTAGATTTTCAGTTTCCATTTCCCGAGAAGGGCAGGGCTATGTAAATAGGCCAGGCCCCGGCAGCCAATGGCTTCAGAGCTTCTGACCAGGTTCTTTGAAGTCTGAGCTTTCTTAGGATCCCACCTAGGAAAGAACTTTTCATAAGGAAACTGATCTGGCTTTCTTGGGGTTTCTTCTGGGGAAAAAAAAAAAAAAAAGGTGTTACATGGCATGTTCTACGGGCAATGTGTACACATAGTAGAAGAGGGGAGACCGAGTCAGGAAACTGCAGAAGGTGGCTGTGGCTTCCAGCTCCTGAAAACGTTTTAGGCGGAACTGCATGTTTTGTTTTGTTTTTCTGTTTTTCTGTTTTTCTGTTTTTCTTTCTTTCTTCCTTTCTTTCTTTCTTTCTTTCTTTTTCTTTTTTTTTTTTTTTTTTTAGAACAGCACGGTTAAGGTTTTGTTTTTCCTTTACCTCTGGGCCTGCAGTTCTTTTTTTTTTTTTAATTTTAATTTTTTTATTAGGTATTTTCCTCATTTACATTTCCAATGCTATCCAAAAAGTCCCCAATACACTCCCCCCACCCCCCTGCAGTTCTAGGATTACAGACAATTCTAACAGAAAGCTCAGACTTCCCAGAAGCCCCCTCTCTTACTCACATCTTACATTTTCCCCTGTGGTGCTGGGGGTGGGGGGCGGTCGGGACGAGAGGGGGGTTTAACCCAGGACATTCCCAAGGTTGAAGTACAGGCTGTACCACCAATTGGGTCAGGACAAGTCAGCAGTGAATATTACAGTGAGCTGTCTTTCAGGGCCACTGCAAGTGTGAAAGTCAGCCTTTGGGTGACACAGGTCCTGGAGGATCACTCATGGGCCACCACCCAAGGCTGCATCAGAATAGTTTGATGACCTCCAACCCCTGCCTCTGTCTCCTCGTCACTTGTTTTGAGTTTTTCACTTCGTAGTTGTTGTGGTCCTGCTGTGAGGCCAAGGCTGGATTTGAACTCATGATTCCTCCAGGTTGGTCCCCCTCAGTGCTGGTGTTACAGGGTGGCCACTGTGCCTAGCTGTGCAGTGGATTGCCTTGGTGCTGTTTGTATTTTGATGCTAATCCTGCCCCAGATGAGGAGCAGATCAAATACTGAAGAGATTTCCATATGAACCTGCTCACCACTTTAAGTGGGCAAAGAAAGGCTAGAGCCTTCGATTGGGCAGTGGAAAAAAAAAAGGCAGGGCAGGAAGTTTTAGATGTGAGAGGGGGGGAGGGGGAGAGGGAGGAAGGAGACTAAGGAGGAGGGGAAGACAAGACAGAGGGACAGAGAGAGGGGAAGATGGAGGAAGATGGAGCAGAACCACGTGACCTGGAGGAGCTGCAAGTAGCAAGGAATCTTATAGCTGGGGAGTAGTGTAATGTTATATGTGCCCAATCTAGGATGTGCACCTTATAAATATTATAACTGAGTTGTGTGTTCTTGGCATGGGCATTTTGGTGTTGAAGATTTACTGATACAAAACTGACTGGTATTCAATATCAAGGCTGTCTGGTGTTTCCCATTTACGACGATTTAGGTGGGCAAGAGAGAGGGCAGAACAGCACGGAGTGGCCACTGCAGGTCTGCAGACCAGCCACAGGGGTAGAGAGCCTGTGGAACTGGAGAGATACCTCCAGGCTTTCTCTGGAAGGACTGGTTTGGGTGGCTTAGCAAGCCAGCCTTGGAAACCAGGGTCAGACAGCCAATTTTTTGGTATCCAGCAAGGAGAAAGGAGCTACTACTGGTCCCCGAGCCTAGTCTGTCCAAGCTTGGGATTTATCAAATTACAGTGTCGTGTGCAATGTGTGTTAACTCATGCCTAAGGCAAAATGTCACCACGAGGGCTCAGGCAGATGAGATAGATGGCGCTATGCTAAACTGTCACCATTCAGCAAGACTATCAGTTTACTGTGTGTGTGCTTCTGTAAATATACAGATAAAGACCCATGAGGGGGTGAAGAATTTGTTCCTTAGCAAATCAACACTGTGGGGCTGGAGACTCAACACTAAGAGCACTGGAGACACTGAGGTCTACTGAGTGCAGGAAAATGAAATTGCGAGCAATCTGGAACCAATGATGACTTTAAGACTTAGTTGTCCATGTGAGGATGAGATTTGAAGCACTGAATCTAATCACAGTGCACTGACTCAAACCCCAAACTAATTTATTCTCTCTCCTTCCTTTCTTAATTTTCTTCTTTCCATATTTCATTCTCTTTTTTTTCCTCTCTATTAACTGGATCTTATTATACATCCAGGTTGGAATTAACAGAACTCCAGGTATCTCGGGGTCCCAACTTCTGGAATTACACACACACACACACACACACACACACACACACACACAGTCACCACAAATCACACAGAGACCTAATGGTTCCACAGGTCAGTGTGCTGAACCAGCATCTCGTGAATACACAGAGACATTAGCCACAGAAGACCCCACATGTGCTTTGTCCACTTGGGAGTGTGAGCTGCAAACTTAGAAGGCAGGAAACTGCTCAGAGCTACCAGCTCCTAGCCCCCTGCTCCTGTTCCCACACCTTATTCCTGTGCCCAATGTCCACTGTGGGAAGCCATATGTGCCGTTGCAGAGTGGCGCTGGCTACTGCTGGCCACAACGCATAAGTTTGGGCAAACAACCAATGTGTACATATGCCGTAAAGTTTTTTGCCAAGACACTGTCTGGCTCGGGCATGATAATGAGGTTCTGAGAGTATAACCAATCAGATGTGAGACATGCAAATGAAGTATGATAATGAGATTCTGTGAAGTACTGAGAGAGAGTAACCAATCAGATGAGGAACATGCAAATGAGGTATAGTGCATAACCAATTCGGGTGTGAGACACGCCTCTCCTAGACCTATATAAGCAGCACCAGTTCTGGGCTCGGGGTCTCTTCCCCTCTGCAATCAAGCTCTCCCAATAAACATGTGCAGAAGGATCCTGTTGCAGCGTCGTTCTTGCTGGTCGAGTCCGGCGCGTGTAAGAGTCCACTCTCAATCTCTGTGAAGGGGGAGTCGGTACATTTCCCTGGCCTTGATTCCTAGAGTTCTCTCTCTAGCAGCCATGAGGACCAGGAGCCCAAATGCCCATGGGTGTTAGAAGATGCATATCCCATGGACAGAGAGCTCTTCACACTCTGCCAGGTGCTCTATCTGAGCCTTCAGGAAACATGGGAATGTGAGTCTATTATTCCAGCCTTGACCCTCTGAAACATCATCAGATCCATGGTTTTAATAAACCATGGCCACAGGTCTCCTGCCAAGCTGTGGACCCATCATGGCCTCACTGAGACTCCCACTTAAGTCCTTTTCTCTTCTACAGCCCCTTCTGCACTCACACTGGACACTAGCTTCCAATTGGACCAGACCCTGCTCTTCTTGGGCCTGGTTCTCGCCTTCAGGTCTGCCATGCAGTGGCTCCCTCATGGTGAGAAGGGAGACACAGGCCACAGCTCCTCCGCCCCATCCCTGACCTCCTCTCCAGCAGGACACCTTCAGAGGAGCCCTGGAAGCACAGCAATTGTCCCAGACCCTGCCCTCCCCTTCTGGTCTGGCTTGTACCTCATTCTTAAGCACCACAGAGAAACAGGGACAGTCTCTGGCCTTGGCATGGCCATGCCTGTGTTTTGATGACATCTGGACACCACTGACAAGGAGAGGCAGAACCTGGGACACACAGCAAACCTGGGTCCCACATTGCTCATTTGGATTGTCCTGTCCTGAGGCATTTGGCATCCTTTCCTGATGGGCTCAATGCACAGTGTGTGGTGGGAGGGTAGAGGCTGAGACTGATGGCTCCAGCCAGTTGAAGACACAGGTGGAGGTGGAGTCTTGATTGTGACACTGGAAAGGACCCACCTGCTGATGTCTGGACTTTGGGTTTCTAACCCAGAGCCTTTCTGCTCTTTAAACCCCATTGTCACTTTGCCACAGGCAAATCTACACAGTTTGTCCCTGCAGCCTGGGATGCACCAGGACCCTTGACTTCTCAATGACACTGCATCTGTCACTCACACACAGAATCTGCTTCTCTTGTTTCTGTTTCTTCTGGGTTTTGTTCCGACCTCTGGGTCTTTGCAGAGTCTGTTCCCAGCACCCTGCATGCTGTTCCACTTTTGCAATTGGCTGGAGGTCCTGAGTTCTTCTGTCCTTGGCTAAGAGGTCCCTGCCTCAGGGAACAACTCAGGAGCCCTTTCCAGGGTCCCAGGTAGAATCAGCTGCCCTCCCTACCTCCCATAGAGTCTTATAAACTGAACTTCCTGAGAAAGGGGAGGAAGAACAAGGAAAGCCATGTGGGTCCTAAAGACTCTCCAAGGCTGAATATCTAATTAGAACTTAGTGATGGAGAGTTGGAATGTTGACAAGTCAGAGCAAAATTATCTTTCATTCTCTTCAGCACTTTTAACAGGCACTCACTGTCTGCCACTGTATCTTATCTAGCAGGGAACCCTGCCAATAAGGACTGGCCTGGCTAGATCTGACCCTAGGTGCATGAGTGGCAGGGATGCTATAGCGAATAACTGAACACATTCTCACTGGATGTGAGGACCACTCCACAGGAGGACACTTCAACCTGGTACTACACATCTGGTGGGGAAGCCACCACTGGGCGATCACAGGCCCCCGGGATGAACCTACCAAAGCGATTTTGGTCAGCAGACAGAATGTCAGGCTGCTCCTTACATCTTTATACCTGATATTATGTAGGCATCCCTCATCTACAGTTCCTGCTTCTCCATGACTCTAGCTTGAGTTTAGTTGGCATTGAACCCAGCCAGTACAGAGTCAGTTTTCCTTGGGGTGTGGTCCCTGGCAGATTACCACTTCCCAGTGGTCGGCTTCACACCTATGTATGAGAAGCACGGACTGGATCCAGTGGTTTACTCATTAAAAGAATCACACTAATTCGGGATGAGGAAGCGTTTTTTGGGAGGAAAGGCGAGGGTGAATTTGATAAAATATATTGAAGGTATATTATGATAACTTCAAAGAATAGTTTACAAAATATACTGGAGGATATTTAATTCCACTATACTTTGCCTTTCAAGAGGCACAAACCACTTCTGCAGAGCTGGTCAGATCCCCTCAGGGGGCTCCTGAGGATATCATGTCTCCAGCAGCTGCCGGACATGTGGCCAAGAGGGCAAATCCAGGTCTTACACCTTGGGACCTGGACACTCCCTGACTCAGTCAGACTTACCATTGAGGTTGGTTTTGTAGCGATGAGTGATCCTTGTTCAGATGCTCGGGAGATCATGGAGGTGGTATAGGTGAGCTGAGCACAGGGACACTCTATAATGCCAGCGACAGAGGCAGAGGCAGGAAGATAGCTGTAAGTGGACTGACAGTCTATAACAGAGATCCTACTTCATAAACAACAGCAAAAAGGGTCACTGAGATAACTGAGAGGGCACAGGTGTTTGCCACCAAGACTGAAGGCCTGAGTTTTGTCCCCAGGACCCACATAGTGGAAGGAGGGGGAGCCAACTCCTACAAGCTGTCCTCTGACCTCACATGCTCACACACAGGAAATAAATGTAATGAACTATCTAAAGCACGATTAGCTTGGCTCTCAGCCAGACCTGCTCAGGACCCTCAGACTCTTGTCCTCCCACCCCGGCACTTGTGGGAGGGGCCTTCAGCACCCCAGTTCCCTCAGGTAAGGTCAGGATCCACCAAGGCAGGTCTGATATCAGCCAAGCCATGAAGATTAGACTTTTTGCTAAGGGCCAGCCACTGACCTGAATCACAAGGCCATGGCTTCTGCATCTTCAAAAAAAAAAAAAAAAACCTTAAAGCCACATATGAATTGAGGTGTCTAAGTTAGGGTTTTACTGCTGTGAACAGACACCATGACCAAGGCAGCTCTTATGAGGACAACACTTAATCGGGCTGGCTTACAGGTTCAGAGGTTCAGTCCATTATCATCAAGGAGGGAACATGGCAACATCCAGGCAGACATGGTGCAGGAGGAGCTGAGAGTTATACTACTTCATGTGAAGGCTGCTAGCAGAATACTGGCTTCCAGGCAGCTAGGATGAGGGTCTTAAAGCCCATGCCCACAGTGACACACCTACTCCAACAATGCCACTCACCTCCCAACAGTACCACTCCCTGGGCCAAGCATACAAACCATCACATGTGGCAAAAAACAATGGGAGAACCAATGTGGTCATAATTTCCTCACTCTTCTGTTCCTTGTTGTCTGCAGCAATTGCAACAGTAATTTAGGTGTGTGCAGTGTGGTGTACATGAGTGTGTGTGCATGTGTGTATGTATACATATGAGTGTGGGAGGGACTTCTTTTTACTGTTTCATGAGACAGTCTCATCATATAGCCCAGGCTGGTCTCAAACTCTGTATCTTCTGTATCAACCTCTCAGACATTGAGATCACAGGTGTGAACCATCCTGCCATGATGAATATTCACTCCTGCTGGGCAGGGAGAGGTTGGCATGGAGACAGGATCACCCTCATGAGCGTGTGTCACAAGTCAACAAGTTAGGCTATTGAAGTGGAGAGTGGCAATTGAGTGAAAACCCAGGTGGTTACAGCTGGGGAGAGAGGCACACCCCTGTGACCTCAGCACTTGGGGGTCCAATGGGGCAGGTCCCCAGGTTTACATCCATCCTGAACAAAGGAGTGAGATCCTAGATTAAAGAAAGATGTCAGACGGATAGACAGAGGTGCGGGTACAGGGCAGAAAGGTTGGTTTGGATTAGGCCTTGGACGATAGGCACATCATGGGTGTGCATCTGAGAACCCACAGACGATAAGTCTGAACTAGAAGTTAGCCAGGACCTTGCAGCATGGACCAGTCAGCTGTGACAAACCCACCACAGCTGTGATGAAGCTGGCGCCTGCCACCTGCTGGATTTCTCTCACTTGCTCTTCACGTTTCAGTAACGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGGTGTTATTTGTGTATGTTCTGTTGTTAAAAATCGTGGAGACTATCAAAGGTTTTTTGTTTTGTTTTGTTTTGTTTTCAGTAACGATTCATGATCAAGAAGGAAGAACAACAGAAACTATGTTCAGGATGCCTCCTGGTCCACAACCTGCTGTGATGGGGGTTTGACAATCCAAAGAAATGCCAGCAGGTAAACAGACCCCAGAGGATCAAGGAGCCCAGGAGCCAGAGGGGGCAGGTGCAGAGGTCTGATCTGCATAGCCCCGCCCTTCCTGGGAAGAGAAAGCAGCTATGGATATAAGGGGCAGCTGACTCCTGAGGTAAGGCTGCAGTCAGCAAACACTCCTGGCCTCAGGATGGCGAGGGAACTCTTCAGAACCCCAATCTGGAGGCTGGATAAGTTCATAGAGGATCAACTCCTTCCTGACACCACCTTCCTTACTGAGCTCAGAGCAGACATCGACTCCATAAGTGCTTTCCTGATGGAGAGATGCTTCCAAGGTGCCGCCCATCCTGTGAGGGTCTCCAGGGTTGTGATGGTGAGTCCCATTAGCCTGAGTTCATTGGGATGGGGTGGGAGAGGACTCTCAGAAGCCAGACTGCAGTCCTTAGTGGGGTGTGAGTTACCTACCTGCTCTGTGCCCAGTGTCCTCATGTGCATCCCAGCTATGGCACAGGGCAGAGGACAGAACAGAGACACCACTGCCTGATGACTGGATGACTGGACAGATGGAAGCTCATTGCCATGTTTCCATTCTGTCACATGGGACTGTATGACAGCAGTCCCTGCCTCAGTCACTCTGAAGGTTGAAGACATGTTGTGTTGTAATGTCCACTCATCAATGTCAGGGGTTGAACTGTGTTAGTGATTGTGGTCAGCATTTTATTTAGGGAGGATCACTACAGATCACTTGTTATCATTTTGTTTGTTTGTTTGTTTGTTTTGTTTTGTTTCGTTTTTCAAGAAAGGGTTTCTCTGAATAGCCCTGGATGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCTTCTGCCTCCCAAGTGCTGGGATCAAAGGCTTGCAACACCACTGCCCAGCTTCCCTATCCTTTCTTATTCTCATTTTTGTCTTTTAGGGTGGCTGCTACAATGAATACACTGTGCTCAAGGGCAGGTCTGAGGCCAACATGGTGGTGTTCCTTATCAATCTCACAAGCTTTGAGGATCAGTTCAACGGACAGGTAGTGTTCATTGAGGAAATTTGGAGACACCTACTCCAGTTGCAGCAAGAGAAACTATGTAAACTCAAGTTTGAGGTCCAGAGCCCAAAGGAGCCCAACTCCAGGTTTCTGAGCTTCAAGCTGAGCTGCCCCGAGCGCCAGCATGAGTTGGAATTTGATGTGCAGCCAGCCTATGATGCCCTGTGTAAGTCAACCATCAGCAGAGCCTCAGTCTGTCCTCCCCTCATGCACGAATCCTCTCTTCTATTTCTGTGTGTGTGTGTGTGTGTGTGTTGGGGGTAGCATGTTCTCATGTATGCAGATGTCTGTTCACATGTTTGCCTGTGTAGGTAATGGTCAGACGCCAGCCTTGGAGGTTGGTTACTTAAGAGCCTATTTTGGTTGAGAGGTTGACCTGGAGTTTGCTGCTCCACCTTCATGGCTCTTGCAGTAAGGAGCTGGGGTCAGGTGGGGTGTGTGCTCTGATGGAGCTGAACCCATGATCTCTGGCACCTGGAGCCTCAGTATATTTATTAGGTACAGCACAGGGCTGGGGGGAGGGGATGCTTGGTCTCCTAGAAGTGTCTGTAGGCTATGAGTCTCCACTTGTAAACAATTATACTCAAACATATTCAGGACAGACTTGGGCTTCCTCTGGGACTCTGAATATGTGTTTACTACATCCATGCACACACAATCCACTCATAACCTCAAAATAGGGAGAGACTGTAAGCAGGAGTTATGATATACAAATGCAGTAGAGTGCTCCATGGTATACCAGAGCATGGTTGGCCATTGACAGATGACTGTGTAGCTCTCTTGTGAGTGACCATAGTGTGTTGTTTATTCCCACCACTGTCTCCAGACTTGTGAGCAATGCAACCTACTATGAGCAGTTGGTGCCACAGATTTTCTAGGTCATTTCACACCATCCAGAGGTGTGGTGGGCTCATGTCTGTATGGGGAGTGATGCTTGCTGCAGACAAACTTTGCCCTGTTCGCCGAAGCCCATAGACAGTGATTGTGACCCTAGAGGTACAGGAGACTCTTAGATCAGAGTTGGGTATAAACATCAATGAAACTTCCTTTATAGATGAAGTAAGACACTTCAAGCCCTTTGACTCCAGTAACTACAACAAAGTCTACGCCCAACTCACCCATGAGTGCACCACACTGGAGAAGGAGGGCGAGTTCTCCATCTGCTTCACCGACCTCCATCAGAGCTTCCTGAGGTATCGTGCGCCCAAGCTCTGGAACCTCATCCGTTTGGTCAAGCACTGGTATCAACTGGTAAGGCATCCGACCCAGATCCTGTGGACCCTATGTTTAGAGAGTGGGATTCAGGCCAGAGAAGAGGTCTAGTCTAGCTGGCTGTCTATGGACATGATAGCTTTATCACATGACCAAGAGCGCCTTGGGAAGGAAAGGTCTCACTTGGTTACACATCCCAGTCACAGGGCATCATTGAGGGAAGCCAGGACAGTAACTGAAGCAAAGACCATGGAGGAATTCTGCACACTACCTTGGTCTCCATGGCTCACTCAGATATTGTACAGTCCACATGCTCAGGCATAGCACCGACCACATCCTCACTTATCATGAATATTTCCGCACAGATCATTAAGGGAGCCCTTCCCTAGGACCGGTTTCTGAACTAGTTTGCTCTGACTGCTGTGGTAAAGATGATGCTGCTGCAAAGTAAATTGGGTGGAAAGGGATTGTCTGGCTCACGTTCCTGCATCGCACTCTATCTCTGAGGGAAGCAGAGGCAGGAAACTATGGCAGAGGCCATGGAAGAACTCTGCTAGCTGTCAGAGACCCTTAGGCGATCAGTCTGATTTTTACAAACAACTCAGGACCACCTGCCCAGGACAGGCAATGCCCACAATGGGTGGGGCCCTCCCACGCTGATCATAAAATGCCCACAGGCTTGACTCCAGGCCAGGCTGATGGAGGCATTTTCTCAGTTGTGGGTCCATCTTCTCCAGATGGCACTCTCTGTGTCAGGTTGACAAAGAGAAATAAAAATGGCACAAGGGGAGAGAATAGAAAATGGAGGAGGAAGCCGGGCAGTGGTGGCGTACGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCAAGGCCAGCCTGGTCTATAGAGTGAGTTCCAGTACAGCCAGGGCTACACAGAGAAACCCTGTCTCAAACAAACGAAGGGAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAATAGAGGAGGAAAAGGGGTTAAATTGGGAGCAGAGCAGAGGGGTTCAGAGAAACAGGGTTTGGGGGATCAGCCAGGGACATGATGCTGTAGAAGGAAGAGGGAATGGGGAGGGAGGACGTCCTGTGCTTGTTCTAAGTGATTTCTTCTAGTCCACTTTCCAATCAGCTAACTCTCTCTTCATCCATGTCTAATTCACTGTTCCATGGTGGCTGCCTCATGTCTGCTTCCTTCTTCAGCAATCCCTGCATGTCACTTTCTATGGCTTATATTTCCTGACATTTCTACCTACTACCTCTTTAAATGGATGACTTCTCTATATTTTTTTTTTCTGGACAATGTTTTACATGTATGTGTGGTGTATGTGTGTGATACCTGTTTGTGGGAGCACACCTCTTTGTGTGTTCTTGTGTGTCCATAAGAATGCAGAGGTCAGCATGTGAGGGCAGGTCCTCAGCCATGCCCCACCTTGTGTAATAAGTCAGGGTCTCACACCTGGCACACCTTCTGCTGGATCAGCAGCGCAAGCCCCTGCAAGCCCCATCTCCAGCCCTTTGAGCTAGGATTAGGAGTCAGCTTCCTCAAGAGCCTGAAACTCACTTAGATGCTGGGGATCTCACCTCTAGTTCTCACCCCTGCACTTTACCCCTGAGCCAACTCCCTGGCCCCTTCCCAGTTATTTCAAAGCCTAGGATCAACCTTCCATAGTGAACACTCCTTATGTCCCACTCCCACCCTCAATCCTAGCTGTCACTCATGGTGTCTTGATTGCTCCTGTGCCTGCTAATGAATGCTTTCTTTCTGAAGCACACAGGGTCAGTCTCAACCACTCAGCTCTTGGCTATAGCAGAGGAGGGGCCGTAGACTGAGTCTGAGTGACTAGGTGTAGCTATGTGACACTGTGTAAGGATATCTTGTGGCAGCCGAATTGAGCCTGTGAGAAGTGTCCCCCACACCAGAGCTGAGAGCCCCGTGTTTCTCATTGCCTTTCTCTTCTCAGTGTAAGGAGAAGCTGAGGGGGCCGCTGCCTCCACAGTACGCCCTGGAGCTGCTCACAGTCTACGTCTGGGAATACGGGATCCACGAAAACCCTGGACTCCATACAGCCCAGTGCTTCCGCACTGTCTTAGAACTTGTCACCAAGTACAAACGGCTTCGAATCTACTGGACATGGTGTTATGACTTTCAACACGAGATCTCTGACTACCTGCAGGGACAGATCAAAAAAGCCAGGTAGCTTGTCCCCACATGGCTCTTGTCCCTGTAGGAGTCAGGCTGCAATCCTGTGAAAAGGGAGAGGTGTGAGCTCTGTCATTGCAGCATCTCCTGCACTGGGTGCTGTCACCTGACTGACCTGAGGTCCTAATGCCCTGGGAGCTGGGGAGGGAGGGTAGGTTATCTTGTCTGTGATGAGGAGACCAAGGCTCTGACATTGTCCAGATCCAAAAGGCAAAGGAAAGGGAAGGACATGGAGTGGGGACTGAGATTTTCCAACAGTGACAGCAATCATAAGGGCTGTGACACACCAAAGACCTGGAGTGGACCAATTACCTGTAAATAATAGAGTGCTATGTCACCCTAGGGAGTGACATGTCCTGATCCTGTGTTTGATCAGTTTTCCCATGAGACTGGCTGGTCCAACTCTCCCCATCTCACAGATGAGAGACCCTGAGGGGCCTGCCCCAGGACACACAGCTACTAAAGGGTAGAGGATGTGAATCCAAGCCCTCTGGGTCATGCCCAAGGATTCCTCCAGTTTGCTGTATGCTGTGGGGCAAGGACTCTGGCCAAGCTTTGGATAGGGATGCAGTGCTGGGCTCCTCCCCTGCCTCTGTGAAGTGGAGCCTGAGGCACTGTTCAGCTGCTCAATTCCCTCCCTGAATGTTGCTCTGCAGGCCTCTGATCCTGGATCCAGCAGACCCAACAAGGAATGTGGCTGGTTCAGACTTACAGGCATGGGACCTGCTGGCAAAGGAGGCTCAGATCTGGATAGATTCGACTTTCTTTACGAACCATGATATGTCCATTGTGGAAGCCTGGGAAGTGATGGTGAGACCCTGTCATCATCCTGTCCCCTTGCACACAGCTTCGGTCACTCCATCTTTTTGGAGAATATTCTCACTGAGGCATGGTCTTTTTCTAAAGATCTCCACACTTCTGTAAAGGCCAGCTGCTTGCTTTCTTTTACAGTTATTTTAGTGCTATTCAGAGCTATCATGGGCTGGCAAGATGGCTCAGTGGGTAAAGGCACTGCCAGCACTGAAGACCTGGCTTTGGTCCTTAGAATTCATAGGGTAGAAGGAAAATAAAACATATTGTCCTCTGACCTTCATCTCTGCTGTTACACAAGTGGACACGCATTCATGCCTATATGCATACATATAAACAGGTGCATACAGACAAACAGATACATAGACTCTGAGAGCTGAGGCCACTGGCTCAGGGACACTCCCCTAGAGAGTGTTAGGACTGGGGTCCCAGGGACTCTGAAGCCACTGTCAGGTTCATGAACAGCCTGTCTTCCCTGTCACATCGTCATGTGGTCAGTGGGATTCAGTGGGGCTGTGTCACAGCCACAGAGGCACAGAGTGACCTGCACTTGGACCTCTCCCCTCCTGGGGCAGTGTCTGCCTGATGCTAGGGAACTGATGATGTCACGTGTGACCTCAGCATATGAGCATAGGTGCAGTGACTTGAGAAAGAAACCTCAGGAGATCAGGAAGGAAGTGAGAGGGGACATGGAGGGAGGCAGCTGAAGGAACAGTGGACTGTAGTGCTGTCCTGCAACAGAGAGGTGGCAAGGCCATGTCTCCCATTTAGCAGATCTATTAGTAAAGAAGAGCCTGCCTGTGGGGCCATATGAACCCTCAATGGCCATAGTCATAAAGGTGTCCTGATGGCCATCCTCCATGTGTGCTGACCATCTTTCTGAGGAGCTCCAGCTGGCCACTCCTGTTACCCACCCTCAGGGCTGATTTACATCCCCCTGCAAGGAATGCAGGCTCCTGACATGATTGTATGTTTTTCATTGCAGCCAGAGAGACAAGAATGTGTCTTCCTGTGAGCACCCCCAGCATCTGCCTAGGAGACTCCAGAGTCAGGGGCATGTCCTCCTCTTCTGTAAGACCTTGACCTAGAGAGGACAGACAGGATGGCACTCAAGGCTCCAGCGAGGGGCATCCAACCTGTGATCAGACTCCAGGCTTCTGATCCCTGCCTGCCCATGGACAGCCTTCCTCACAGGCTGCTTCGTCTGCCTTAGCTTCCAACAGTGTTCTCTGGGAGTCAGACTGTGATGGACAGAGAAGAACGCAAGCTCGACTTCCATCTGTTCACCTGTTGGGAGGTTATGTCCAATAGTGGCTGATCATCATCAACAAACCACAGCAAGCCATGAGGGGGGGTGCACTCTGAGGGAGGAGTCCTCAGACCACACAGAAACTTTTCAGCAGTGCATGTGGCCCTGGAGCCCTGGGAATCTGGCCAGTGTTCATCAAGGTGCACTGTTTCTGCAACATGCAGGCTGGGTTTATGGTAGTGCAGGAAAATAAAATTGCATGCATTTTAAAATTTATGATTTTAAAACTTAGGGGTGTGTGTGTATGAGATTTGAAGCACTAAATTAAAGCAAAACGCATTGAATTAAACTAAATGAATTAATTTATTCTCCCTCCCTCCTTTCCTTTCTCTCTTTCTGCCTGCCTTCCTTCCAACTGGATCTTATATGTCTCACCCTAGTTTGTAATTTTACAGAATGCCACCTGCCTCAAATTTCTATGTTCTGGAGCTATACACACACCACCACCACCACCACCACCACCACCACTGCCACCACCACCAGCAGCACCACCCTTCACATACAGGCTAAACGGTTCCATAGTTCAGTGTGTTGAACCAACAGTTTTACAAAGG
D3_15 121340140 121355188 15049 ATATCCCATGAACTCAGAGCCCTTTACACTGCCTGGTGCTCTATCTGAGCCTTCGGGGGACATGGGAATGCTGCTCTATGATTCCAGCCTTGCCCCTTTGAAACACCACCAGAAACATGGTTTTAATAAACCATGGCCAAGGTCTCCTGCCAAGCTGTGAACCCATCATAGCCTCACTGAGATTCCCATGTAAGTCCTTTTCTCTTCTACAGCCCCTTCAGCACTCACACTGGACACTAGCTTCCCATTGGACCAGACCCTGCTCTTCTTGGGCCTGGTTCTCACCTTCCGGTCTGCCATGCAGTGGCTCCCTCATGGTGAGAAGGGAGACACAGACCAAAGCCCCTCCCCCCACCCTTGACCTCCTCTCCAGCAGAGGAGCCCTGGAAGCACAGCAATTGTCCCAGACCCTGCCCTCCCCTTCTGGTCTGGCTTATACCTCATTCTCAAGCCCCACAGAGAAACATGGACAGTCTCTGGTCTTGGCATGGCCATGTCTGTGTTTTGGTGACATCTGGACCCCACTGGCAAGGAGAGTCAGAACCTGGGACACACAGCAAACCTGGGTCCCACGTTGCTCATTTGGATGGCCCTGTCCTGAGGCATTTGGCATCCTTTCCTGATGGGCTCAATGCACAGAGTGTGGTGGGAGGGTAGAGGCTGAGACTGATGGCTCCAGCCAGCTGAAGACACAGGTGGAGGTGGAGTCTTGATTGTGACACTGGAAAGGACCCACCTGCTGATGTCTGGACTTTGGGTTTCTAACCCAGAGCCTTTCTGCTCTTTAAACCCCATTGTCACTTTTCCACAGACAAATCTACACAGTTTGTCCCTGCAACCTGGGATCCACCAGGACCCTTGATTCTCAGTGATTCTGGGTCTGTCTCACACACAGAATCTACTACTCTTGTTCTGTTTCTTCTGATTTTTGTTACCACCTCTGGGTCTTTGCAGAGTCTGTTCCCAGCACCCTGCATGCTGTTCCACTTCTGCAACTGGATTGAGGTCCTGAGTTCTTCTGTCCTTGGCTAAGAGGTCCCTGCCTCAGGGAGCAACTCAGGAGCCCTTTCCGGGATCCTGGGTAGAGTCAGCTGCCCTCCCCACCTCCCATAGAGTCTTATAAACCGAACTTCCTGAGAAAGGGGAAGAAGAACAAGACAGAAAGCCACGTGGGTCCTAAAGACTCTCCAAGGCTGAATATCTAATTAGAACTTAGTGATGTTGGCAAGTCAGAGCAAAATGACCATAGGCTCTCTTCAGTGTTTTAACAGGCATTCAGGCTTGCCACTGTGTCTTACCCAGCAGGGAACCCTGCCAATAAGGACTGGCCTGGCTAGATCTGTCACTGGGCAGGGATTCTTTAAGGAATAACTGGACACATTCTCACTGGATGTGAGGACCACTCCACAGGAGGACACTCCAACCTGGTACTGCACATCAGGTGGGGAAGCCACCACTGGGCGATCACAGGCCCCAGGGATAAATGTCAGGCTGCTCCTTACATCTTTATACCTGATATTATGTAGGCATCCCTCATCTACAGTTCCTGCTTCTCCATGACTCTAGCTTATGTTTATTTGGCATTGAACCCATCCAGGACAAAGTTCTTTTTCCTTGGGGTGTGGTCACTGGCAGATTACCCACTTCCCAGTGGTTGGCTTCACACCTGTGTATGAGAAGCATGGACTGGATCCAGTGGTTTAATCATTAAAAGAGTCACACTAATTTGGGATGGGGAAGTGTTCCTTGGGAGGAAAGGGGGGGTAAATTTGATAAAATATATTGAAGGTATATTATGATAACTTCAAAGAATAGTATACAAAATGTCCAGGAGGATATTCAATTCCACTATATTTTGCCTTTCAAGAGGCACAAACCACTTCTGCAGAGCTGGTCAGATCCCCTCAGGGGGCTCCTGAGGATATCATGTCTCCAGCAGCTGCTGGACATGTGGCCAAGAGGGCAAATCCAGGTCTTACACCTTGGGACCTGGGCACTCCCTGACTCAGTCAGACTTACTGAAGAGGTTGTCTGCATAGATGAGTGACCCTTGTTCAGAGGCTCGGGAGAGCTCAAAAGTGGAATAGATCAGCTGGGCACAGTGACACAGTATAATGCTAGCAACAGGGGTTGAGGCAGGAGGATAGCTGTAAGTGGACTGACAGCCAGCCTATAACAGAGGTCCTGTCTCATAAACAGCAGCAAATTGCTTCACTGAGATAGCTCAAAGGGTACAGGTGTTTGTCATTAACCTTGATGGCAGGACCCCTGTGGTGGAAGGAGAGAGAGCCGACTCCTACAAGCTGTCCTCTGACCTCACATGCTCACACACAGGAAATAAATGTCATAGACTATCTAAAGCAGGATTAGCTTGGCTCTCAGACAGACCTGCTCAGGACCCTCAGCCTCCCGTCCTCCCACCCTGGTTCTTGTGGGAGGGGCCTTCAGCATCCCAGTTCCCTCAGACAAGGTCAGGATCCATCAAGGTGGCTTTGATATCAGCCAGGCCATGAAGACTACTTTTTGCTAAGGGCCAGCCACTGGCCTGAATGAAAAGGCCATGGCTTCTGCTGTTTTCCTAAGAAAATCCCTTAAATCCACCTATGAGTTGTGGTATCTCAGTTAGGGTTTTACTGCTGTGAACAGACACCATGACCAAGGCAACTCTTATAAGGACAACATTTAATCAGGGCTGGATTACAGGTTCAGAGGTTCAGTCCATTATCATCAAGGAGGGAACAGCATTCAGGCAAGCATGGTGCAGGAAGAGCTGAGAGTTCTACATTTTTATCTGAAAGTGAAAGAACCTAGTGGGGAAACCCCCACTCAATTGTCGTTCGGCGCGCACCCAAGAATCACAAACAGACAACCATCTTGATGTAAAAGCACGAGGTAGTTTAATGACAGAGCTCTGGGCCGACACATATCTCCCGCAGGAGACAGAGGTGTCGACCACATGGTGGAGCCAAAATGGCGGAGCTCCGGTTCGAAACGTATCTCACACAGGAGACAGTGGTTTCGACCCAGATGCTTGGAAGCTAGGGGTTTTTATAGAAAAGGGGTGGGGCTGGGGGAGGAATTGGCTTGGTTTCACATGATTGGTTCATTTAAACATCAACAGACTGTCAGAAGGGCGGGAGATAGGGAGGCACTAGGCCAGTCAGGACATGTGACGATGGGCCTGCCCGGGCATGTCCTGGCCTGTTCTGCTATGTTTTCAGCCCCAGGTTTCAAAGCTCACAAACAACTCTTTGGGCTATTTGACACAAATTACATGAATCACATGTCTCAAGTATTATTTCCTTTCAAAAGCTGCTAGCAGAATACTGAATACTCCAGGAAGCTAGGACAAGTGTCTTAAAGTGCACACCCATGGTGTGTGTGTGTGTGTGTGTGTGTGTGTCTGTAGTGTTTGTGTAGTGTGTGTAGTGTGTGTGTGTAGTATGAGGTGAGGGTGGGCTTTCAGGCCTGCCAGAGAGTCACCAGGTTCCTGCCTGAGTCGAGCACAAGATCCCTTCTTTCCTCCACTATGTGTGCTTGTAACCTGCTCAAAAGTCAGTTAACCATCCTGAGCTTTGATCTTCCCAAATGTCTTATAACCCCTGTGAATGCAGTCAAATCAGCACAGATGAGGTGAGCTGACAGATGGCCACAGAGCAGGGAAGCAGTCTGCAGACATTTGTGGAAATGTGCTCACACAGGCACCCAAAGGTGTGTGTCCACGTACATCTAAGGCTAGTGAAGTTGGCCATGAAGATGCGCCATTTGAAGGAACCCTGAGGGTTGATTTGGGAGGAGGGCAGTGGCTGTCAGAGTGGATCCCTCCCCCAATTACTGACCTGAATCCAGTTTCAGTGTTGAAACCAGGAGTGTAGATGAAGAGGCTTCCTGGGGAACAGAACCCTGTGGGACCATGGTGGACAGAGTAGTGCGTGCCCTTGGTGGGCATATGCTACTTACCGAGAGGGTGGGCACTGGAGAGAAGGGAGTGTCTGACCTCGACAGAAGGTCAAAGTTGAAGACAGGGCTTGGCACCCCAAAGTGTCTGTTGTCAAACCCTGCCACTTCCCTGCCCAGGGTGGATGGTAAGTCAGCACCATAGGACATCCCTAGGGAAGAGCAGGGATGAGCACCATGCTTAGGGCAAAGGGATCTTGTCAGATCCAGTCTGGAGCAAGCATGATGCTGCACAACATGGCAGTCAGATGCTCTAATATGCAAGTGTCAACTATGTGCCACACACTCTGGTCATTAAATTATTAAGTCAGCTTGACAAGCCAAGAGGCTTGGAAGCTACTCACTGAGGCAGAAGAGTTTCACGGAAACTTACTCCTGGAGTCTGGCGTTCTCTCTAACCCATACATTAATTTTCCATTTCCGAGTTAATCTAGTGTATGGATCCACATGCTCTCTTTCAGTATTATCCTATTATAAGTGCCTTTTAAGATTGAATTCTGACATAGCTAAAGCCTTCTGCCAGTGTTCCAACAATCCTAAAACGTCCTTGAGCAAGACCATGGGCTTGGAATTCAATAAGAGTGTTGCCTATTCAGTGACATTCAGAATAGCCTCTGGTACTACAATACCATTTAGAATAAAAGCCAAGTTGCACCCATATACAGGAATTTACAATGGTTTAGGAAGTAGATGGGCTGTGGTTTTAGAGTATTGCATTATTCATGAGATGGTTAGCTAGTAAGGAACTCCCTAGTTAGAACCACTACTTGGGTGTGAAAGCCCTTGCCCTAGGAGAGTAAAAACCTCACACCTGGCTTCTAAGAGTATAGCTGAGTTACACCCATACACACGCATTTACAATGGCCTAAGGGGAAGGTGGACTCTAAAATAGGAACTATGGCAAGGGCACGAAGCCCTTGACCCAGGCTTCTAAGATTAAGTGAATCTTAGGTGGAGCCCTTGTTGATCCCAGTTGTTAACAATGAATTCTATCCCAGGTGGTTCCTGTTAGACCTTCTGTGTTTGCATTCCTCTGTTTTATGTAAGAATCCAGTAACCTCTTTGTACCTTGCTGGTGTGTCACCCAACTTCCTTATTGTCTTCTGCATAAAAAGTCTGATGCTAGATTTGACAAATTACATTCAGATCCTACACATCCTCTCCTGTGTGCATCTGTCTTTCAATTCATCCAATGCTTTGCCGACCAGAGACTAGAGACCCTTTCCACGCAGACAAAGGGGCCCAGAGGGTCTGCAGCAAATATGGACATCTTTCTCCCTCTGAGAGACTCTGCATCCCTGTTGTCATGGAAACGATCCAGCCACCATGAGGCTCCTCCTCCCTTAAGCTGCCCATTGCTCATTCCTGTACACTGCTGTTTAACTCCTTGCTGTGTATCATTGGGGAGGCTCTCAAAGGTCATCACAAGGGTTCATGCTGTCCTTCATGGGGAAATGGGGTGTGGCCCTTACACATCACATGTGGGCTTGCTTTCACATGGAGTCCATGAAAGCTGACATATTGATTCCCCGCTGCTTGGCAAGAGACATTGGGATGCCCTGGAGATTGATTATTAACACCGACTCTGTGTGTCTGGGGTCGCTTAAGAAAGAGCAATACTGAAATCATAGACGAGCAACACTGGGTCCCCCGAGGGCTCAGATAAAGGACACAGCACAGGGACATGAATCTTCTTACACCCGGTATGCTGGCACTCCTCTTTGTGAGGGACACTGAGTAAAAACTGAGTGAACATGGGCTGGGAGGACACACAAACAAGCACACGCGCACACACACACAGAGTTATTCAGAGAGTGGGTATTAGTGCCAGGAGAAGGCTTTGAGAGCTGGTGGTCCTTCACAATGCCTTGAATTCTAGTGAGCAGGTGTGCATGAGGCGAGTACACACATGAGTCCTTTGAGTCTGAGAAATGACCAGATGGTTCACAACACTGATGGATGGGACCATTAGGTCTCTGTGAGGAAGGGTGTGTGTGTGTGTGGGGGGGGGGTGATTCCAAAACCAAGTGAGAGACTGAGGCAGGAGGATTCCTGTGAGTTCCAGGACTGCATGTATCACATCATGAATTCATGTCTCAGAAAGGAAGCCACAATCATTGTGATTTAACTAAATGTGATAAAGCTGATCGTTGATACCAATGACCCTGTTTCAAAGTTACATGTGTCAAAAATTCCAGGTACCTTTTATTTTCATCATATCTATGGTCCCCCAGCCTGCATGTTGTAGCCACTCAAGGGCACCCTGAGAGCACAGGCCACAATCTCAGTGTTCCATGTCCATGTGTAGCCAGAGAACCGCTAATGTGTCAGATAGGGCAGGGCTAGAATCTTGTCTTGGGGTCATGGGCAGAAGAAACCAAGGGTTCCTCTCTTGTGTGTATGTATAAGAAGAGACCTGGAAGGATCCCAATCCAACTTGCATCAAAAAGCCTAGCAGTTAGGCTTTGCTCTAACCACTGCTGAACATCTGAATCATGTAGCATATGTAGTAAGTGCTCACTAAATGCATCTAGTTGAACCGTGAAGACACACACGCATGTGCATGGGCACACACACGCTCACACACCTGCACTGTTCTTGTATGGCCCACTCCTCAGAGTTCACACACCATGACGGCACCTGCTGTGGTTTATTGTTGATCAGACTTTGTCAGACAGAACCTTCCAACAGGTGGACAGATGGACACCAAGCGTGTGTTCTTTCCTCTCCTTCTGAATCTGTTGAGGAAGGCTGGCTGTGATTGGACAGGAGTCAAACATGGCTGGCAGGCATCAGAAGCACGGAGTCTGATGCCAGGTGGGGCCCTCCCTCACTGGGTCATGGGCCTGCAGCAGAGCAGCAAATGCCCCTGACCAGCAGTCTCCTGGGCAGGTGCTGCTGTGCTCACAGCAGGATACATGTCCAGTTCTCTTCTACCTGAGGATGGAGGGAAGGTAGAGCATGGTCAAAACATCAAGCTTACCCTGCTGTCACCCCTGCACTGTGACAGGAGAATGCCAAGATCCAGGCCAGCCTGGGGCACACAGCAAAAACCCACCTCACACCAACCACAACAAAGAACAAATGCTAACCTGCTCAAAAGGTACAGGAACCACCGTCTGCAATGAAAAATAACACAATCGTGTCAGGAGCCTGCATTCCTACATGAAGGGGGTGTAAATAAATCCTGGTTATGGGGTACCATGACTATACACCTGGTGCTCCTCAGAAAGGGGGCAGCACACTCAAGGCCATCAAGATTCCTTTATGACTATGCCATTAAGGGTACATAAGACCCCCCAGAAGGGCCCTTCTTCATTAGTAGATCTGCCACGTGGGAGACATGGACTAGCCCCCTCTCAGTTGCAGGACAGCACTACAGGCCACTGTTCCCACAGCTGCCTCCCTCCATGTCCCCTCTCACTTCCTTTTGACCTCCTAAGGTTTCTTCCCCACATCACTGCACCCATGCTCATCCCCACCCTGAGGTCACACGTGACATCATCAGTTCCCTAGCATCACGCAGACCCTGCCCCAGGAGGGGAGAAGTTCAAGTGCAGGTCCCTCTGTGCCTCTGTAGCTGTGACACAGCCCCGCTGAGTCCCATTGGTCACATGACAATGAGTCAGGGAAGACAGGATGCTTCATGGACCAGACAGTGGCTTCAGAGTCCAAAGGATTCCAGCCCTGCCAATCACTAGCTGAATGTCCCTGAGCCAGTGGCCTCAGTTCTGAATCTCCTTTACACTCTCTCCATGAGGGTCAGTGTGTGGGTGCATGTGTGTGTGTGTATGTATGTATGCATGTATGTATATGTCTGTGTTTTATATGAGTGTGTGTTTCTGTGTATGTGTGTTTGAGTTTGTGTAAATATAAGTGCATTTGCATGAGTGTGCATTGTGTGAGTTTCTGTGTGTGTCCAGGAGTGTAGCAGCACACAGATGAAGGTCACAGGACAACATGGGGGATTTGTCTTTTTCCTTCTACCATATTGGTTCCAGGAACCAAACCCAGGTCTTCAGTGTTGGCAGTGCCTTTACCCATTAAGTCATCTTGCCAGCCCATGATAGCTTGAATGGTGCTGAAATGGCTGTAAAATAAGGTTATCAGCTGGCCCCCCACAGAGAGTGAAGTCCTTTAGCAAAAGACCACATCTCAGTGAGAATAATCTCCAAATAGATTGAGTGACTGAAGCTGTGTGCAAGTGGACAGGATGATGATGGGGTCTCACCGGCACATCCCAGGAGCTCACTCGGGAACCATCCTTTTTAATAAAACATGGGTACCATAGCCACACATCAGCCTCTTCAGCCAACCGCCTCCAGCCCTCTGGGTTCCCACCGGCCACATTCCCTGTTGGGTCAGCTGGGTCCAGGATCACAGGCCTGCAGAGGAGACAGGCACTGTTCAGCTCTCCAGTGCCCCAGGAAACACTGACAGGGGCAGGAGAGGCACCTAGCAAAGCATCCCTCTCTAAAGTCTCTCCTTTGTCTGGGCCCCACCGTGTAAGGTAAGCCTGGAGGGACCCTTTGGCATGACGTATAAGGCCTGGGTTCAAATACTCTATCTTTTAGTAGCTGTGTGTCCTGTGACACACAGGCTCCCTGATCTGTGACATGGGGAGAGTTGGACCAGCCAGTCTCATTGGAAAACTGATCAAACACTGGATCAGGACATGTCACCCCCTAGGGTGACACAGTACTCTATTATTTACAGCTTGCTAATCACTTCAATTTATGCTGGAATATTTCAGTTTTCACTCCATGTCCTCCCCATCCTTTGCTGCTTCATTTTGACAATGTCAGAGCCTTGGTCTCCTCATCTGCAGACAGGAGAGTCCAGCTCTGCCTCCCAGGGCTGTAGGACCTCATGTCAATCAGGTGACAGCAAGACAGTGCCCAGTGCTGGAGATGCTTCCTAGACAGAGCTCCCCACACAAGTGCAGCCTGGTACCAATGGGGAGCAAAGCCATGTGTGGACACTACCTGGCTTTTCTGAGCTGTCTGTGCAGGTATTTGGAGACCTCCTGGTGTTGAAAGTCATAATACTTTGTCCAGTAGATTCGAAGATGCTGATAATTGATGACCAGTTCCAAGACGGTCCGGAAGCCCTGGGCTGTGTTGAACTCATAACATCCATTCCCTTGTTCCCAGGCAAAGACAGTGAGCAACTCTAGGGCGTACTGTGGAGGCAATGGCTTCCCCAGCTTCTCCTTACACTGAGAAGAGAAACACAATGAGAAAATATGGGTCTCTCAGCTCTGGTGAGGGGGACACTTCTCACAGGCTCAATTCAGCTGCCATCTGATAAAGGAATTGCAGGTTTATTGTCACGCAGCCACATCCACTCACTTAGATTCAGTCTCTGGACCATCCTGTGCTATAGTCAAGAGCTGAGTGGTTGAGACTGACACTGTGTGCTCCACAAAGACAGCATTCATTAGCAGGCACAGGAGGAATCAGGACACCATGAGTGACAGCTAGGAGTGAGGGTGGGAGTGGGACATAAGGAGTACTTACTACTGAAGGTTGATCCTGGGCTTTGAAATAACTGGGATGGGGACAGGGACATGGTTAAGAGTAATGCACCTCCCTCCCTGTGCAAGGGTGAGAACTGGAGGTCAGATCCTCAGCATCTCCATGAGTGTCAGGCTCTTGAGGAAGCTGACTCCTAATCCCAATTCAAACAGGTAGAGCTGGGGGTCCCCCAGGCTGTGTGACTGCCCAGTACTGCTGATTCAGCACATGCTGTGTCCTGTGTAAGACCCTGACTGATTATACAAGGTGGGGATTGCCTGTTGACCTGCCCTCATACTCTGCCCTCAAATGCTCATGAACATGCATACCCAAATACACAACATGTGCTCCTATGAATAGCTCTAAACACACACATATAAAAAACACTCAGAATATAACAAGAAATCACACATTTAAAAACACAGCTTGACAAGTCAGGAAAATGAAAGTCTAAAAGGTGCCATGAAGGGTTAGCCAAAGATGAAAGAAAAATCCAAAGACTCAAATGAAGCAGCAGCCATGGAACAGTGAATCAGACACAGCTGAAGAGAGAGTTAGCGGATTGGAAAGTGGACTAGAAGAAATCACCTAGAACAGGCAAGGAAAATCCTCCCTCCTCTCAGATACCCCTCCCCATTCTTTGTCCCTCCTACAGCATCATCTCCCCCCATTTCCACTCACCCCTGGTTCTCTGTCACCCCCTTCCCTCCCCTCTGATTCCTTTCCACCACTTTTTCTTTTACATTGTTTTTTTTTTCTCACTGCTTTTTGCCAAATTTTATTCTTTTTGTCAACCTGACACAGAGAAAGTCATCTGGAGAAGATGGACCCACAACTGAGAAAATGCCTCCTCGATGTTGGTCCTTGGGAGGATTGAGAAAGGATTTTTCATCTTTGGGTTGAAAAGCTATTGAGAGCTGAGAGTGGACTCTGTTTGTGGGAACTTGGAAGACAGAAGTGCTGAGAAGCAGGCAGGTGACAGTGGCTAGAGTGAAGTTTCAGAGAAAAGCAAAGACCCCACCTTGGCTCTTTGTGTGCTATGTTTGGAGTAAGAATCAGCAATTTCTCATCAGCTGGGGCTAAAAACCAACTCTATTTAAGAAGAAACCTGCACCACGGGGGTCAAGTGTTTGGAAAGAGTTTTCTTCTGGTCAGAAAACAGTACCTGTGGTCCAGGGATCCTAAGGCAGCATCTCCAGTTGGCAGACACACCTAGAACTACAGAGAGGTCTCCCAGGGGTTAAAGCTTTAGTCTCATGAGAGCTGCAGGTTTGGAGGGGTCATGGGAAGCAGCTGAGGCCTGGCACTGAGAGAGGCCAGGAGAAGCCATTGGTGAAAGGGCAGCCTCAATGGCAGTGGAGGCCCTGAGATACTGGACAGGAGACAACTGCCAGGAACAGAGCAATGTTAGAACAGAGCTGGCCTGAGCCTGAGGTGTGTTGTGTGTGCTGTGGATGGCAGAGCCTGAGAAGGTCAGAGAACTGTGAGTCCCAGATATCAGATACTGAGCCTTTTGCTCTGTTGGGCTTTGGTTACGTTTTAATTAGATTGTGACAGTGAACTGGTTCTTCCCTCTTGCAGTAAGAAAGTATTTAACTTGTGTTTTTAATAGTATATGAGCCCACCATTGAGAAACTAATCTTTGTAAGTGTCATAGTTTCTTTAGGTTTGGTTTTTAATTTGGGGACTTTAAAACTCCATTGGGTTTTATGATGTGATACTGACATGTGATCTTAGGGACAAGTAAGAAGGGAAAGGTTATGGTTCAACTGTGATTTGTTTGTGTGCCATGTTGAAAAGGGGTCAATTGTGCTGCTTAATTTTTGTCCACTAAACACCAGCTAGCCTCATCTGGCAGGAGTCCATCTTGCTCAGTAAAATGACTATCCATCTGACTGGCTTGGATAGTTTATTGGCATGTTACTAATTACTGATTGATGTGGGTGGAAACAGACCACTGTTGTTGGGGCCATGAGTGGAATGTGGCCTTGGGCTATATAGAAATGCCCACTGAGTGAGCCATGGACAGCAAACCGTGAGAAGAATCCCTCCATGGCCTCTGCTTCAGTTATTCCTCTAACTTCCCTCAATGATGGAGTATGCCTTAGATGTGTAAACATAAGAATATCCTTCCTCCCCAGAAAAGTTTTGACCACATGATAAAGCTATGACCTCAAAAGAAAGCAAACTAGAATATACATTACCTGTGGCCTGTGTCTACACTCTGTACAGATGAAGCCCACAGTGGCTGGGCCAGATGCCTTACCAGTTGGTACCAGTGCTTGACCAGGCGGATGAGACTCTTCAGCTTGGTTGGGCGCTGCTTCAGGAAGTTCCGCTGGAGCTCCGTGAAGCAGGTAGAGAACTCGCCATCCTTCCCCAGGGAGGTACATTCCTCGATGAGGATGGCATAGATTCTGGGATCAGGCTTGCTGGAAGTATTAACATGACCTATAAAGGAGATTTCATTCATTTTAATATCTCAGCCATTCTATGAGGTTCCTCAGTCTCCCCAGGTCTAGAATCACTGCCTATGGTGTTCAGTGAACACGCAGAGTTTCTCCAAAGCAGACATCACTCCACATTCACTCCCAACTACCCATCAGCCCCACCACTCCTCAGGAGTGCACACATGCTGTGCACTACAAAGTCCATGCTCCCAGTTTGTTCTAGTGAGAGGGTCTTTCTTGCAGGCCTGGAGACAGTGGTGGGAAGAACCAGCACACTGTGGTCACACACACGGTTGTCCGATAATGGTCAACCATTATCCAGTATCTGGGTTCAGCATCTTCTGCATGGTTCTTGTCAACGCTGTGCACTCATGTATGTGTCAAAGTTTACCGTGGGGCAGTCGGCTGCACTGGTATATCATGTCTCACGCTGACAGAGTATCTGCCCTATTTTGTGTATAGAAACTAAGGTTTCACTGGGTTTTCACTGAACTATTTTCTGTAGTGTGTGACTTAATATGGTGTGGTTTTGTTTGTTTGAGCCCTGAAAAGAACAAGATACGGCAGTGTTCCTCAGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGTGTGTCCTGTGTACTTTGTACAGTGTATGTGCAACAGATATTAGTGTAGGTGTATGCACACGTGTGAGTAGTCTAGGCAGGCTGTGCCTTAAGTGTGTGCACACGTGTGACATGCAGCAGGCCACATCATCACACTGTGATGCTGACACAGGGCAAAGTCACACAAGGATGCAGCTTAGAGAAGCCAGTCCCTCAGTGACCAGGGAGGTCTCTCTTTCACTGGCTTATTTTCAGTGTCAGGGTCTAGAATACAATGAAGCTTATCACCTACAGAATACAAGAGACAAAAGAGAGAATCTCAGGGGCAGAAGATACCAAAGAAAAGATTAACACAAAATAAAAAAAAAATGCAAAAAGCAAAAAGCTCCTAACCCAAAACATCTAGAAAATCCAGGACATAATGAGAAGATGAAACCTAAAGATGATAGGTATAGAAGAGAGTGAAGATTCCCCACTCAAAAGGCCAGTAATTATCTTCAACAAAAATATAGAAGAAAACTTCCCTAACTAAAGAAAGGGATGCCCATGAACGTACAAGAAGCCTACAGAACTCCAAACAGACTGGACCAGAAAAGAAATTCCTTCCATCACATAATAATCAAAAAACCAAAAGCACTAAACAAAGAAAGAATATAAAAAGCAGTAAGGAAAAAAGGTGAAGTAACATATAAAGTCAGACGTATCAGAATTATACCAGACCTCACCAGAGACTATGAAAGCTAGAAGATCCTGGATAGATCACATACAGACCCTAAGAGAACACAAATGCCAGCCCAAACTACTATACCCAGCAAAACTCTCAATTACCATAGATGGAGAAACCAAGGTATTCCATTTCAAAACTAAATTTACACATATCTTTCCATAAATTCAGCCTTACAAAGGATAATAGATGGAAAACACCAACACAAGGATGGAAACTACACCCTAGAAAAAGCAAGAAAGTAATCTTTCAACAAACCCAAAAGAAGATAGCCACAGAAACATAAATCCACCTCTACCAACAAAAATAACAAGAAGTAACAATCACTTTTCCTTAATATCTCTTAACATCAATGGACTCAATTCCCTCACAAAGACATAGACTAACATACTGGATGTGTAAACAGGATCCAGCATTGTGCTGCACACAAGAAACACACCTCAGTGTAAAAGCACTACCTCACAGTAAAGACACAGACTACCTCAAAGTAAAAAGTTGGAAAACAATTTTCCAAGAAATGGTCCCAAGAAACAAGCTGAAGTAGTCATTCTAATATAAAACAAAATCAACTCTCAACCAAAAGTTTTCATAAAAAGATAAGGAAGGACACTTCATACTGGTCAAAGGAAAAATCAACCAAGATGAACTCTCAATTCTGAACATCTATGCTCCAAATGCAAGGATATCCACATTCATAAAAGAAACTTACTAAAGCTCAAAGCACACATCACACCCCACACAATAATAGTGGGAAATTTCATCACCCTACTCTCAGCAATGGACAGATCATGGAAACAGAAATTAAACAGGGACACAGTGAAATTAACAGAAGTTATGGACCACATGGATCTAACAGATATTGATAGAACATTTCATCCTAAAGGAAAAGAATATACCTTCTTCTCAGCACCTCAAGGTACCTTCCCCAAAATTGACCATATAATTGCTCACAAAACAGGCCTCAGCAGATACAAGAAGACTGAAATAATCTGATGAACCCTATCAGATCACCATGGACTAAGGCTGGTCTTAAATACCAACAAAAACAACAGAAAGCACACATACACATGGAAATTGAACAACATTCTACTCAATGATAACTTGGTCAAGGAAGAAATAAAGAAAAAAAAAATTAAAGGCTTTCTAGAATTTAATGAAAATGAAGACACATCATACCAAAACTTATGGGACACAATGAAAGCAGTGGGTAAGAGGAAAACTCATACCTCTAAGTACCTTCAAAAAGAAACTGAAGACCTGAAAGCTCTAGAACAAAAAGAAGCAAATACACTTGATAGGAGTAAATGGCAGGAAATAATCAAACCAAATAGAAACAAAAAGAACTCTACAAAGACTCAACAAAACCAGGAAGTGTTTCTGTGAGAAAATCAACAAGATAGGTAAACCCTTAGCCGTACTAACAGAGACCACATCCAAACTAACAAAATCAGAAATGAAAAGGGATACAGAACAATGAAGTATACCTTAAAATAACTATTCTGTTTGTTGAATATCATGTTCTACAAACATCATGGAAATATTACTCATAATTTTACTCACTGTGCTTGAAGTTAGCATTTTTGTAATGTTTAACTTCAAAGAGTTTTTGCTATTTTAAAATTTATAAAATATATTTACTGATAAAATAATTTCTCTCCTAGAAACACTGGTAATCTTTTTTAAGTAAACTGATTGTTAGACCATGTACACAGATATATAATGCCTTTCAAATACTCTCTTACTATGTCAGTGGTATCATATAAGGGCTTTTGAATATATTTCTTAATGACCCATTTTAAATA
GF3 -1 -1 1 CTGGTCCATGCTGCAAGGTCCTGGCTAAC
GF4 -1 -1 1 CAATAGCCTAACTTGTTGACTTGTGACAC
EX52 -1 -1 1 GCACATGTGTGTTGCATAGGTGTTCACATG
EX32 -1 -1 1 GAAAATAGAGATGAATTACTCTAGAGCTG
GR3 -1 -1 1 CTGCTTCAGGAAGTTCCGCTGGAGCTCCG
GR4 -1 -1 1 CTAGCCTCATCTGGCAGGAGTCCATCTTG
Comments:
Created
Target: 2803Ensembl ENSMUSG00000052776 -- 121355605 -- 121355893 ENSMUSE00000473372 -- ENSMUSE00000473372
creating candidate regions
attempting to insert candidate regions into db
done inserting candidate regions
running aos on us and ds
running aos on gaps
finished running aos
AOS Produced 8 oligo candidates from u/d regions
AOS Produced 14 oligo candidates altogether
inserted results of aos runs
Aligned target to NCBIM37 assembly: chr: 5, start 121355605: end 121355893
Found 11 RP24 black6 bacs on NCBIM36
Found enough RP24 bacs: not looking for RP23
D31:5:121355175:121355294_47 was rejected with 2 alignments
U31:5:121356773:121356837_0 was rejected with 3 alignments
Entered all non-repetitive oligos
Sequence count reduced by 2 by bac-alignments
finished aligning oligos to bac
ACCEPT (0) gap pair G5:5:121359893:121362892_2848 G3:5:121351105:121352604_919
ACCEPT (0) gap pair G5:5:121359893:121362892_2848 G3:5:121351105:121352604_912
REJECT (2) gap pair G5:5:121359893:121362892_2848 G3:5:121351105:121352604_904
ACCEPT (0) gap pair G5:5:121359893:121362892_2843 G3:5:121351105:121352604_919
ACCEPT (0) gap pair G5:5:121359893:121362892_2843 G3:5:121351105:121352604_912
REJECT (2) gap pair G5:5:121359893:121362892_2843 G3:5:121351105:121352604_904
ACCEPT (0) gap pair G5:5:121359893:121362892_2539 G3:5:121351105:121352604_919
ACCEPT (0) gap pair G5:5:121359893:121362892_2539 G3:5:121351105:121352604_912
REJECT (3) gap pair G5:5:121359893:121362892_2539 G3:5:121351105:121352604_904
DESIGN COMPLETE
U-type: U2
D-type: D2
Creating display features: mapping to reference assembly
Created display features
Inserted short and long range genomic sequence arms

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