Contact WTSI Webmaster Printer friendly format Login to WTSI resources WTSI RSS feed
Functional Genomics
  • Human (HGP)
  • Pathogens
  • Blast
  • Mouse Genomics
  • Information
  • MICER end reads
  • Clones and AB2.2 cells
  • WTSI MTA form
  • Micer FAQ
  • Bradley Lab
  • Website Search
  • People Search
  • Library Services
  • Site Map
  • Feedback / Help
Mouse Genomics - MICER
The Bradley Lab - Mutagenic Insertion and Chromosome Engineering Resource (MICER)
Overview

The aim of this resource is to provide vector sequences and information on using MICER vectors for generating knockout mice, and for chromosome engineering. Frequently Asked Questions FAQ

Vector Maps and Sequences

  • 5'HPRT [MHPN] (Map & Sequence)
  • 3'HPRT [MHPP] (Map & Sequence)
  • 5'HPRT [MHPN] and 3'HPRT [MHPP] (Sequences only)

ES Cell Protocols

  • Embyonic Stem Cells - General Notes
  • Electroporation of ES Cells
  • Picking ES Cell Colonies and Transferring Them to 96-Well Plates
  • Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
  • Freezing and Thawing Cultured Cells

Other Resources

Ensembl Genome Browser:

Ensembl Genome Browser

Ensembl produces and maintains automatic annotation on selected eukaryotic genomes

Ensembl Trace Server:

Ensembl Trace Server

Public archive of over a billion primary sequence trace files

Requests for AB2.2 ES cells

Sanger Clone Request form for AB2.2 ES cells

N.B. Before any biological materials can be sent a Material Transfer Agreement (MTA) form must be completed, signed and received by us. Please download the MTA form from here, complete it and send by fax the original signed copy to the address provided on the MTA form.

Clone Requests

Please read for changes in clone provision (2006).

Please read for changes in clone provision (2007).


Publications
A system for rapid generation of coat color-tagged knockouts and defined chromosomal rearrangements in mice.
Zheng B, Mills AA, Bradley A
Nucleic Acids Res. 1999;27;2354-60. PMID: 10325425
Engineering chromosomal rearrangements in mice.
Yu Y, Bradley A
Nat Rev Genet. 2001;2;780-90. PMID: 11584294
Mutagenic insertion and chromosome engineering resource (MICER).
Adams DJ, Biggs PJ, Cox T, Davies R, van der Weyden L, Jonkers J, Smith J, Plumb B, Taylor R, Nishijima I, Yu Y, Rogers J, Bradley A
Nat Genet. 2004;36;867-71. PMID: 15235602
Information Projects Other Services
Sanger Home
Sitemap
Site Search
Information
Careers
Press
News
Seminars
Workshops
Publications
Staff Theses
Travel Directions
Research Teams
Research Faculty
Personnel Search
Human Genetics
Model Organism Genetics
Pathogen Genetics
Bioinformatics
Sequencing
Library
Helpdesk
Webmail
VPN Access
Sign In
SSO Pass. Reset

webmaster@sanger.ac.uk

Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK  Tel:+44 (0)1223 834244

Last Modified Mon Oct 15 11:09:23 2007

Genome Research Limited is a charity registered in England with number 1021457

Data Sharing Policy | Conditions of Use | Copyright