Vector Information

The vector designs used in creating the SIGTR are shown below. Each vector is engineered in each of the three reading frames designated by the number in the vector name. For example, pGT0lxf represents the frame "0" vector in which the exon upstream of the insertion donates a complete codon. Frame 1 and frame 2 vectors donate 1 and 2 bases, respectively

For more detailed information on a particular vector, follow the links below to access the full sequence of the vector in FASTA format, the annotated sequence or a restriction map of each vector.

The loxP and FRT sites contained in the vector enable post-insertional modification of the trapped locus. For more information on post-insertional modification contact SIGTR.

Vector Information
pGT0lxf
Fasta	Annotated sequence	Map (pdf)
pGT0lxr
Fasta	Annotated sequence	Map (pdf)

Intron: 1.5 kb of Mouse En2 intron 1
SA: splice acceptor of mouse En2 exon 2
β-geo: fusion of β-galactosidase and neomycin transferase
pA: SV40 polyadenylation signal