General Information

Full description for handling SIGTR cell line requests with embedded protocols.

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Media

Media preparation for tissue culture.

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ES Cell Culture

Protocols used in the culture of SIGTR cell lines. Includes thawing, expansion, and freezing protocols.

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RT-PCR

Protocol for RT-PCR confirmation of gene trap cell lines and also useful for genotyping gene trap mice.

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X-Gal Staining

Reagent preparation and protocol for detecting β-glactocidase reporter expression in cells, embryos and tissues.

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Dot Blot Hybridization

Protocol for genotyping gene trap mice that can be used for any gene trap insertion.

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